Biomedical Research-tokyo最新文献

Erratum to: Efficacy of 1,5-anhydro-D-fructose on reducing mental stress: a randomized, double-blind, placebo-controlled trial-a pilot study. 1,5-无水d -果糖对减轻精神压力的功效：一项随机、双盲、安慰剂对照试验-一项初步研究。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.210

引用次数: 0

Green coffee bean extract rich in chlorogenic acids prevents muscle capillary regression via inhibiting oxidative stress and enhancing angiogenesis. 富含绿原酸的绿咖啡豆提取物通过抑制氧化应激和促进血管生成来防止肌肉毛细血管萎缩。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.15

Jihao Xing, Hao Lin, Ryosuke Nakanishi, Kazuya Iwai, Noriaki Maeshige, Hiroyo Kondo, Hidemi Fujino

{"title":"Green coffee bean extract rich in chlorogenic acids prevents muscle capillary regression via inhibiting oxidative stress and enhancing angiogenesis.","authors":"Jihao Xing, Hao Lin, Ryosuke Nakanishi, Kazuya Iwai, Noriaki Maeshige, Hiroyo Kondo, Hidemi Fujino","doi":"10.2220/biomedres.46.15","DOIUrl":"10.2220/biomedres.46.15","url":null,"abstract":"Reactive oxygen species (ROS) production in disused skeletal muscle induces capillary regression, which disrupts the balance of pro- and anti-angiogenic factors. We tested whether green coffee bean extract containing rich chlorogenic acid, which has antioxidant properties, can prevent capillary regression caused by muscle atrophy. The study included 24 female Sprague-Dawley rats, randomly assigned to four groups: control (CON), 2-week hindlimb unloading (HU), 2-week hindlimb unloading supplemented with coffee extract rich in chlorogenic acids (HU+50%CGA), and 2-week hindlimb unloading supplemented with trace amounts of chlorogenic acids (HU+5%CGA). Rats in the HU+50%CGA and HU+5%CGA groups received an oral dose of coffee extract at 850 mg/kg per day. The effects of chlorogenic acids in coffee extracts were investigated by comparing these groups. Unloading increased oxidative stress, disrupted mitochondrial oxidative activity, and upregulated TSP-1 expression, triggering endothelial cell apoptosis and leading to capillary regression. In contrast, the supplementation with coffee extract containing rich chlorogenic acids prevented ROS overproduction and improved metabolic activity, promoting angiogenesis by correcting the imbalance of pro- and anti-angiogenic factors, and inhibiting endothelial cell apoptosis. In conclusion, green coffee bean extract rich in chlorogenic acids inhibits ROS production, enhances mitochondrial metabolism, mitigates capillary regression by promoting angiogenesis and preventing apoptosis.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 1","pages":"15-25"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastric biopsy-derived cell line and its utility in assessing tumor cell drug sensitivity. 胃活检来源的细胞系及其在评估肿瘤细胞药物敏感性中的应用。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.27

Masato Hayashi, Rei Noguchi, Makoto Abe, Julia Osaki, Yuki Adachi, Shuhei Iwata, Kazuki Sasaki, Tadashi Kondo, Yuki Yoshimatsu

{"title":"Gastric biopsy-derived cell line and its utility in assessing tumor cell drug sensitivity.","authors":"Masato Hayashi, Rei Noguchi, Makoto Abe, Julia Osaki, Yuki Adachi, Shuhei Iwata, Kazuki Sasaki, Tadashi Kondo, Yuki Yoshimatsu","doi":"10.2220/biomedres.46.27","DOIUrl":"10.2220/biomedres.46.27","url":null,"abstract":"Gastric cancer (GC) has benefited from treatment improvements such as minimally invasive surgery, molecular-targeted drugs, and immune check point inhibitors. However, the prognosis of advanced GC is still unfavorable. Minimally invasive pre-treatment detection of drug sensitivity (MI-PDDS) has increasing importance in view of improved chemotherapy. Gastric biopsy specimens are obtained with relative ease but have not been considered an appropriate source for generating cell lines because of their minute amounts. We therefore materialized the idea of MI-PDDS using biopsy-derived cell lines obtained from endoscopic biopsy specimens. Here, a cell line designated TCC-GC1-C1 was established from a biopsy specimen of a histologically confirmed adenocarcinoma of the stomach. The cell line showed the ability of forming spheroid with deeply stained nuclei and disturbed cellular morphology indicative of malignancy. Single-nucleotide polymorphism (SNP) genotyping of the cell line revealed a duplication of chromosome19q and a deletion of chromosome 8p. A drug screening test with 221 anticancer drugs showed that the cell line had high sensitivity to the proteasome inhibitor (Carfilzomib) and the fibroblast growth factor receptor inhibitor (Erdafitinib), with a low IC50 value of under 0.1 μM. Our MI-PDDS approach holds promise in making a treatment decision for advanced GC.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 1","pages":"27-35"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in 5-hydroxytryptamine and its metabolite, 5-hydroxyindole-acetic acid, levels in the brain of rats administered with methotrexate. 5-羟色胺及其代谢物5-羟吲哚乙酸在大鼠大脑中的变化。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.77

Sachiko Hiraide, Takuji Machida, Mina Yamada, Honoka Furukawa, Takahiro Yamamoto, Naoya Hamaue, Kenji Iizuka

引用次数: 0

Intragastric administration of cuminaldehyde, a natural TRPA1 agonist, enhanced carbohydrate oxidation and reduced body fat accumulation in mice. 小鼠灌胃茴香醛（一种天然TRPA1激动剂）可增强碳水化合物氧化并减少体脂肪积累。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.51

Fuminori Kawabata, Ren Fukai, Taiga Miyagi, Yuko Kawabata

{"title":"Intragastric administration of cuminaldehyde, a natural TRPA1 agonist, enhanced carbohydrate oxidation and reduced body fat accumulation in mice.","authors":"Fuminori Kawabata, Ren Fukai, Taiga Miyagi, Yuko Kawabata","doi":"10.2220/biomedres.46.51","DOIUrl":"10.2220/biomedres.46.51","url":null,"abstract":"Transient receptor potential ankyrin 1 (TRPA1) is a nociceptive receptor activated by chemical compounds such as allyl isothiocyanate, cinnamaldehyde, and carvacrol, found in spices and herbs. Since the intake of some TRPA1 agonists inhibits visceral fat accumulation, TRPA1 agonists are expected to serve as ingredients in anti-obesity foods. Recently, it has been reported that anisaldehyde, tiglic aldehyde, and cuminaldehyde activate TRPA1. In the present study, we measured metabolic changes after a single intragastric administration of each of these three compounds by respiratory gas analysis in mice, revealing cuminaldehyde's outstanding carbohydrate metabolism-enhancing effect. Next, mice were fed a high-fat diet for 4 weeks, during which they received daily administration of either cuminaldehyde or vehicle. The cuminaldehyde-treated group exhibited a significant suppression of visceral fat accumulation. Respiratory gas analysis confirmed that oxygen consumption and fat oxidation in normal conditions were significantly higher in the cuminaldehyde group than in the vehicle group at 2 weeks post-administration. These results suggest that the intake of cuminaldehyde, a TRPA1 agonist, enhances energy metabolism and reduces visceral fat accumulation.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 2","pages":"51-59"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mouse experimental periodontitis by silk-ligature placement triggers systemic inflammation potentially exacerbating pulmonary inflammation. 小鼠实验性牙周炎通过丝绸结扎放置触发全身炎症可能加剧肺部炎症。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.61

Shingo Kawai, Hiroyuki Oguchi, Mayumi Endo, Koji Hase, Shunsuke Kimura

{"title":"The mouse experimental periodontitis by silk-ligature placement triggers systemic inflammation potentially exacerbating pulmonary inflammation.","authors":"Shingo Kawai, Hiroyuki Oguchi, Mayumi Endo, Koji Hase, Shunsuke Kimura","doi":"10.2220/biomedres.46.61","DOIUrl":"10.2220/biomedres.46.61","url":null,"abstract":"Periodontitis, a chronic inflammatory disease driven by oral microbiota dysbiosis, is associated with systemic diseases; however, the mechanisms remain poorly understood. Using a silk-ligation-induced periodontitis mouse model, we investigated the changes in oral microbiota, immune responses, and systemic effects, including respiratory disease. Oral dysbiosis persisted for five weeks and was characterized by an increased abundance of genera Enterococcus, Proteus, and Escherichia_Shigella, and a decrease in genera Lactococcus. Antibiotic treatment effectively reduced alveolar bone resorption and suppressed both oral and systemic inflammation, suggesting that the dysbiosis induced innate immune activation in the gingiva which led to alveolar bone resorption and systemic immune activation. Notably, while gingival inflammation and bone resorption subsided within 2-3 weeks, systemic immune activation persisted throughout the experimental period with elevated levels of Th17 cytokines, particularly IL-17A and IL-22. To further examine the systemic impact, we induced pulmonary fibrosis with bleomycin after the onset of periodontitis. Periodontitis exacerbated initial lung inflammation, marked by increased production of IL-17A, IL-22, and IL-1β, along with significant T-cell infiltration in lung tissue. These findings reveal a chronic systemic inflammation induced by periodontitis and highlight its potential role in exacerbating respiratory diseases.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 2","pages":"61-76"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible involvement of neuropeptide Y sub-receptor 1 (NPY-Y1) in the anti-viral response of SARS-CoV-2 infection in Syrian hamster. 神经肽Y亚受体1 （NPY-Y1）可能参与叙利亚仓鼠对SARS-CoV-2感染的抗病毒反应

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.37

Haruka Nishimura, Kohei Araki, Chihomi Mitsuoka, Wataru Toriumi, Shunichi Kitajima, Eiki Takahashi

{"title":"Possible involvement of neuropeptide Y sub-receptor 1 (NPY-Y1) in the anti-viral response of SARS-CoV-2 infection in Syrian hamster.","authors":"Haruka Nishimura, Kohei Araki, Chihomi Mitsuoka, Wataru Toriumi, Shunichi Kitajima, Eiki Takahashi","doi":"10.2220/biomedres.46.37","DOIUrl":"10.2220/biomedres.46.37","url":null,"abstract":"Co-administration of Molnupiravir and Remdesivir, treatments for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), inhibits viral replication and infectivity. Previous studies indicate that the neuropeptide Y sub-receptor 1 (NPY-Y1) is involved in influenza virus aggravation in mouse pulmonary phagocytes, but the exact mechanisms remain unclear. Understanding the NPY-Y1 receptor's involvement in influenza and SARS-CoV-2 in both mice and hamsters may help explore its potential as an indicator of viral infections and support the development of preventive care. This study examined the effects of Molnupiravir and Remdesivir on infected Syrian hamsters and the NPY pathway during SARS-CoV-2 infection. SARS-CoV-2 infection increased mRNA expression of NPY, NPY-Y1 receptors, and inflammatory cytokines and chemokines in hamster lungs. Co-administration of the drugs significantly reduced these expressions. Changes in NPY-Y1 receptor expression were correlated with NPY, IL-10, IL-12, and IFN-γ, implying a role in the antiviral response pathway. These findings highlight that changes in the mRNA expression levels of NPY and NPY-Y1 receptor are influenced by SARS-CoV-2 infection and that the antiviral drugs impact the NPY-NPY-Y1 receptor cascade. This implies the pathway's involvement in inflammatory responses during viral infection and its potential as a therapeutic target.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 2","pages":"37-50"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial DNA copy number variations in Epstein-Barr virus-transformed B cells after exposure to radiation: A possible biomarker for dose assessment. Epstein-Barr病毒转化的B细胞暴露于辐射后的线粒体DNA拷贝数变化：剂量评估的可能生物标志物。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.9

Hitomi Muramatsu, Ryosuke Seino, Hisanori Fukunaga

引用次数: 0

Mycophenolate mofetil reduces cell viability associated with the miR-205-PAX9 pathway in human lip fibroblast cells. 霉酚酸酯降低人唇成纤维细胞中与miR-205-PAX9通路相关的细胞活力。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.1

Hiroki Yoshioka, Hanane Horita, Yosuke Tsukiboshi, Hisaka Kurita, Yurie Mikami, Kenichi Ogata, Aya Ogata

{"title":"Mycophenolate mofetil reduces cell viability associated with the miR-205-PAX9 pathway in human lip fibroblast cells.","authors":"Hiroki Yoshioka, Hanane Horita, Yosuke Tsukiboshi, Hisaka Kurita, Yurie Mikami, Kenichi Ogata, Aya Ogata","doi":"10.2220/biomedres.46.1","DOIUrl":"10.2220/biomedres.46.1","url":null,"abstract":"Cleft lip is a birth defect associated with environmental and genetic factors. Recently, microRNAs (miRNAs) have been reported to play a crucial role in lip formation, with the disruption of miRNAs influencing the development of cleft lip. Exposure to medicinal agents in pregnant women is one of the reasons for cleft lip. Although an association between pharmaceuticals-induced cleft lip and miRNAs has been suggested, it remains to be fully elucidated. This study aimed to clarify the molecular mechanism of mycophenolate mofetil (MPM)-induced inhibition of cell proliferation and miRNA expression in human lip fibroblast (KD) cells. Cell viability, apoptosis, and cell cycle-related markers were evaluated after 72 h of MPM treatment. In addition, miRNA levels and the expression of their downstream genes were measured, and a rescue experiment was performed by overexpressing PAX9. We showed that MPM dose-dependently reduced the viability of KD cells. In addition, MPM treatment suppressed cyclin-D1 and cyclin dependent kinase-6 expression in KD cells. Furthermore, MPM upregulated miR-205 expression and downregulated the expression of PAX9 (downstream gene). Moreover, PAX9 overexpression alleviated MPM-induced inhibition of cell proliferation. These results suggest that MPM suppresses cell viability by modulating miR-205-PAX9 expression.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"46 1","pages":"1-8"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium phosphate controls nucleation and growth of calcium oxalate crystal phases in kidney stones. 磷酸钙控制着肾结石中草酸钙晶相的成核和生长。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.103

Uta Michibata, Mihoko Maruyama, Yutaro Tanaka, Masashi Yoshimura, Hiroshi Yoshikawa, Kazufumi Takano, Yoshihiro Furukawa, Koichi Momma, Rie Tajiri, Kazumi Taguchi, Shuzo Hamamoto, Atsushi Okada, Kenjiro Kohri, Takahiro Yasui, Shigeyoshi Usami, Masashi Imanishi, Yusuke Mori

{"title":"Calcium phosphate controls nucleation and growth of calcium oxalate crystal phases in kidney stones.","authors":"Uta Michibata, Mihoko Maruyama, Yutaro Tanaka, Masashi Yoshimura, Hiroshi Yoshikawa, Kazufumi Takano, Yoshihiro Furukawa, Koichi Momma, Rie Tajiri, Kazumi Taguchi, Shuzo Hamamoto, Atsushi Okada, Kenjiro Kohri, Takahiro Yasui, Shigeyoshi Usami, Masashi Imanishi, Yusuke Mori","doi":"10.2220/biomedres.45.103","DOIUrl":"https://doi.org/10.2220/biomedres.45.103","url":null,"abstract":"Kidney stone disease is a serious disease due to the severe pain it causes, high morbidity, and high recurrence rate. Notably, calcium oxalate stones are the most common type of kidney stone. Calcium oxalate appears in two forms in kidney stones: the stable phase, monohydrate (COM), and the metastable phase, dihydrate (COD). Particularly, COM stones with concentric structures are hard and difficult to treat. However, the factor determining the growth of either COM or COD crystals in the urine, which is supersaturated for both phases, remains unclear. This study shows that calcium phosphate ingredients preferentially induce COM crystal nucleation and growth, by observing and analyzing kidney stones containing both COM and COD crystals. The forms of calcium phosphate are not limited to Randall's plaques (1-2 mm size aggregates, which contain calcium phosphate nanoparticles and proteins, and form in the renal papilla). For example, aggregates of strip-shaped calcium phosphate crystals and fields of dispersed calcium phosphate microcrystals (nano to micrometer order) also promote the growth of concentric COM structures. This suggests that patients who excrete urine with a higher quantity of calcium phosphate crystals may be more prone to forming hard and troublesome COM stones.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 3","pages":"103-113"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0