Biomedical Research-tokyo最新文献_第2页

Bone regeneration-enhancing effects of extremely low-frequency electromag- netic fields: Analysis using fish scales as a bone model. 极低频电场对骨骼再生的促进作用：以鱼鳞为骨骼模型的分析。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.187

Nobuo Suzuki, Makiko Kakikawa, Yuta Oda, Jingjing Kobayashi-Sun, Sotoshi Yamada, Kouhei Kuroda, Isao Kobayashi, Masato Honda, Hajime Matsubara, Yoshiaki Tabuchi, Nobuaki Shimizu, Kazuki Watanabe, Jun Hirayama, Atsuhiko Hattori

{"title":"Bone regeneration-enhancing effects of extremely low-frequency electromag- netic fields: Analysis using fish scales as a bone model.","authors":"Nobuo Suzuki, Makiko Kakikawa, Yuta Oda, Jingjing Kobayashi-Sun, Sotoshi Yamada, Kouhei Kuroda, Isao Kobayashi, Masato Honda, Hajime Matsubara, Yoshiaki Tabuchi, Nobuaki Shimizu, Kazuki Watanabe, Jun Hirayama, Atsuhiko Hattori","doi":"10.2220/biomedres.45.187","DOIUrl":"https://doi.org/10.2220/biomedres.45.187","url":null,"abstract":"Electromagnetic fields (EMFs) noninvasively promote fracture healing, prevent osteoporosis, promote diaphyseal growth, enhance differentiation, and stimulate cell division. However, no good model systems for analyzing bone regeneration have been reported. In this study, we examined the in vivo regeneration of scales having osteoblasts and osteoclasts using a new magnetic field generator for exposing aquatic animals to EMFs at a sine-wave frequency of 60 Hz. Goldfish scales were put into a fish-breeding space with the developed magnetic field generator and exposed to extremely low-frequency electromagnetic fields (ELF-EMFs) of 60 Hz at an intensity of 1, 3, and 5 mT for 10 days while being regenerated the scales. After exposure, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities in the goldfish scales were measured as markers of osteoblasts and osteoclasts, respectively. As a result, both ALP and TRAP activities in regenerating scales exposed to 3 mT ELF-EMFs were higher than those in regenerating scales exposed to 1 and 5 mT ELF-EMFs. Exposure of scales to 3 mT ELF-EMFs significantly enhanced the scale regeneration rate. Exposure of rat calvaria to 3 mT ELF-EMFs also increased both ALP and TRAP activities like in goldfish scales. Thus, we concluded that 3 mT ELF-EMFs contribute to the medical treatment of bone diseases.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 5","pages":"187-195"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of membrane proteins targeted by small-molecule compounds using nanomagnetic beads. 利用纳米磁珠鉴定小分子化合物靶向的膜蛋白。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.179

Yasufumi Kikuchi, Takayuki Ando, Tadashi Ashizawa, Akira Iizuka, Akari Kanematsu, Chie Maeda, Chikako Hozumi, Haruo Miyata, Kazue Yamashita, Tomoatsu Ikeya, Ken Yamaguchi, Yasuto Akiyama

{"title":"Identification of membrane proteins targeted by small-molecule compounds using nanomagnetic beads.","authors":"Yasufumi Kikuchi, Takayuki Ando, Tadashi Ashizawa, Akira Iizuka, Akari Kanematsu, Chie Maeda, Chikako Hozumi, Haruo Miyata, Kazue Yamashita, Tomoatsu Ikeya, Ken Yamaguchi, Yasuto Akiyama","doi":"10.2220/biomedres.45.179","DOIUrl":"https://doi.org/10.2220/biomedres.45.179","url":null,"abstract":"In drug discovery research, it is important to identify target proteins of bioactive small-molecule compounds and analyse their functions. In this study, we examined whether target membrane proteins could be captured by compounds that bind to membrane proteins on the cell surface. For this purpose, we performed affinity purification using the compound-immobilized nanomagnetic beads. Affinity purification with nanomagnetic beads is known to be effective for determining the protein binding partners of small molecules. However, most previous studies have targeted proteins in the cytoplasm. As a model compound, we chose BMS-1166 (a representative small-molecule compound from Bristol Myers Squibb), a PD-1/PD-L1 immune checkpoint inhibitor that binds to PD- L1 and promotes PD-L1 dimerization. BMS-1166-immobilized beads were manufactured and incubated with extracts of cells with high PD-L1 protein expression. The bound protein was confirmed by western blotting and proteomic analysis to be PD-L1. BMS-1166-immobilized nano-magnetic beads were able to specifically bind and capture the membrane protein PD-L1. In addition, high-purity protein could be obtained from cell extracts in a single step. This is the first report of the purification of a membrane protein to high purity with nanobeads. Nanomagnetic beads with immobilized compounds are an effective tool for identifying the protein binding partners of small molecules, especially when the targets are membrane proteins.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 5","pages":"179-186"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper-induced renal toxicity controlled by period1 through modulation of Atox1 in mice. 铜诱导的小鼠肾毒性通过调节 Atox1 受 period1 控制。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.143

Sarah Tominaga, Hiroki Yoshioka, Satoshi Yokota, Yosuke Tsukiboshi, Masumi Suzui, Makoto Nagai, Hirokazu Hara, Nobuhiko Miura, Tohru Maeda

{"title":"Copper-induced renal toxicity controlled by period1 through modulation of Atox1 in mice.","authors":"Sarah Tominaga, Hiroki Yoshioka, Satoshi Yokota, Yosuke Tsukiboshi, Masumi Suzui, Makoto Nagai, Hirokazu Hara, Nobuhiko Miura, Tohru Maeda","doi":"10.2220/biomedres.45.143","DOIUrl":"10.2220/biomedres.45.143","url":null,"abstract":"Copper (Cu) is known to induce oxidative stress and apoptosis in the liver, kidney, and brain. We previously demonstrated the molecular mechanism underlying the Cu-induced hepatic diurnal variation. However, the cellular molecule(s) involved in Cu-induced renal chronotoxicity remain unknown. In this study, we aimed to elucidate the molecular mechanisms underlying Cu-induced diurnal toxicity in the kidneys. We evaluated cell viability and clock gene expression levels in mouse renal cortex tubular cells (MuRTE61 cells) after Cu treatment. We also examined the Cu homeostasis- and apoptosis-related gene levels after period 1 (Per1) overexpression in MuRTE61 cells. Cu treatment decreased MuRTE61 cell viability in a dose-dependent manner. It increased the Per1 expression levels after 24 h. Notably, Per1 overexpression alleviated the Cu-induced inhibition of MuRTE61 cell viability. Moreover, Per1 overexpression downregulated the cleaved caspase-3 and reduced Cu levels by upregulating the antioxidant 1 copper chaperone (Atox1) levels. These results suggest that Cu-induced renal toxicity is associated with Per1 expression via the regulation of the copper chaperone, Atox1.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 4","pages":"143-149"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of linalool on respiratory neuron activity in the brainstem-spinal cord preparation from newborn rats. 芳樟醇对新生大鼠脑干脊髓制备物中呼吸神经元活动的影响

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.151

Yuka Shibuya, Kayo Tsuzawa, Hiroshi Onimaru, Masahiko Izumizaki

{"title":"Effects of linalool on respiratory neuron activity in the brainstem-spinal cord preparation from newborn rats.","authors":"Yuka Shibuya, Kayo Tsuzawa, Hiroshi Onimaru, Masahiko Izumizaki","doi":"10.2220/biomedres.45.151","DOIUrl":"https://doi.org/10.2220/biomedres.45.151","url":null,"abstract":"Linalool and linalyl acetate are major components of lavender essential oil. These substances possess many biological activities, such as anti-inflammatory activity, analgesic and anxiolytic effects, and anticonvulsant properties, and they also induce modulation of neuronal activity in the autonomic nervous system. However, there are no reports of the direct effects of linalool on respiratory activity. In the present study, we analyzed the effects of linalool and linalyl acetate on central respiratory activity in the brainstem-spinal cord preparation isolated from newborn rats. Linalool dose-dependently decreased the rate of respiratory activity. This effect was reversed by bicuculline, suggesting that linalool enhanced inhibitory synaptic connections via GABAA receptors. In addition, linalool reduced the coefficient of variation of inspiratory burst intervals and thus could work to stabilize the respiratory rhythm. Linalyl acetate did not cause inhibitory effects as observed in linalool treatment. Linalool depressed burst activity of pre-inspiratory neurons in the medullary respiratory networks and increased the amplitude of inspiratory inhibitory postsynaptic potentials of pre-inspiratory neurons. We concluded that linalool caused inhibitory effects on respiratory rhythm generation mainly through activation of presynaptic GABAA receptors of pre-inspiratory neurons.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 4","pages":"151-161"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blockade of CD80/CD86-CD28 co-stimulation augments the inhibitory function of peptide antigen-specific regulatory T cells. 阻断 CD80/CD86-CD28 协同刺激可增强肽抗原特异性调节性 T 细胞的抑制功能。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.115

Yui Maehara, Kazuyoshi Takeda, Kyoko Tsuji-Yogo, Kodai Morimoto, Masaki Harada, Kyohei Kuriyama, Saori Hirota, Hideo Yagita, Ko Okumura, Koichiro Uchida

{"title":"Blockade of CD80/CD86-CD28 co-stimulation augments the inhibitory function of peptide antigen-specific regulatory T cells.","authors":"Yui Maehara, Kazuyoshi Takeda, Kyoko Tsuji-Yogo, Kodai Morimoto, Masaki Harada, Kyohei Kuriyama, Saori Hirota, Hideo Yagita, Ko Okumura, Koichiro Uchida","doi":"10.2220/biomedres.45.115","DOIUrl":"10.2220/biomedres.45.115","url":null,"abstract":"Mixed lymphocyte culture under the blockade of CD80/CD86-CD28 co-stimulation induces anergic (completely hyporesponsive) T cells with immune suppressive function (inducible suppressing T cells: iTS cells). Previously, iTS cell therapy has demonstrated outstanding benefits in clinical trials for organ transplantation. Here, we examined whether peptide antigen-specific iTS cells are inducible. DO 11.10 iTS cells were obtained from splenocytes of BALB/c DO 11.10 mice by stimulation with OVA peptide and antagonistic anti-CD80/CD86 mAbs. When DO 11.10 iTS or Foxp3- DO 11.10 iTS cells were stimulated with OVA, these cells produced IL-13, but not IL-4. DO 11.10 iTS cells decreased IL-4 and increased IL-13 production from OVA-stimulated naïve DO 11.10 splenocytes. When Foxp3+ DO 11.10 iTS cells were prepared, these cells significantly inhibited the production of IL-4 and IL-13 compared with freshly isolated Foxp3+ DO 11.10 T cells. Moreover, an increase in the population expressing OX40, ICOS, and 4-1BB suggested activation of Foxp3+ DO 11.10 iTS cells. Thus, blockade of CD80/CD86-CD28 co-stimulation during peptide antigen stimulation augments the inhibitory function of Foxp3+ regulatory T cells, and does not induce anergic Foxp3- conventional T cells. Peptide-specific Foxp3+ regulatory iTS cells could be useful for the treatment of allergic and autoimmune diseases without adverse effects.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 3","pages":"115-123"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analgesic effects of linalyl acetate via nociceptive TRPV1 inhibition in mice. 乙酸芳樟酯通过抑制小鼠痛觉 TRPV1 产生镇痛效果。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.217

Miho Hashimoto, Kenji Takahashi, Toshio Ohta

{"title":"Analgesic effects of linalyl acetate via nociceptive TRPV1 inhibition in mice.","authors":"Miho Hashimoto, Kenji Takahashi, Toshio Ohta","doi":"10.2220/biomedres.45.217","DOIUrl":"https://doi.org/10.2220/biomedres.45.217","url":null,"abstract":"Transient receptor potential vanilloid 1 (TRPV1) is primarily expressed in sensory neurons and functions as a nociceptive channel. TRPV1 is activated by capsaicin, acidic pH, and noxious heat. Compounds inhibiting TRPV1 have been explored to develop analgesic drugs. In this study, the effect of linalyl acetate (LA), a lavender essential oil component that exerts analgesic effects, on TRPV1 was investigated by measuring intracellular Ca2+ concentration ([Ca2+]i) and whole-cell membrane currents. The analgesic effects of LA on TRPV1-mediated pain were also examined. LA inhibited [Ca2+]i responses to capsaicin, acidic pH, and heat in mouse sensory neurons. Unlike the transient LA-inhibition on capsaicin- and heat-responses, its inhibition on acid-responses persisted even after the LA removal. In TRPV1-expressing HEK293 cells, LA reversibly suppressed [Ca2+]i responses to capsaicin and heat, but persistently inhibited those to acids. Similarly, LA reversibly attenuated current responses to capsaicin but durably suppressed those to acids. LA sustainingly inhibited the responses to spermine, an endogenous TRPV1 agonist, and reduced pain-related behaviors induced by spermine and noxious heat. These results indicate that LA inhibits TRPV1 in a mode-independent manner, with long-lasting inhibition of acid-induced TRPV1 activation. These inhibitory actions of LA on TRPV1 may be related to its analgesic effects.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 6","pages":"217-230"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotonin reuptake inhibitor suppresses the activation of human platelets by a combination of thrombopoietin and collagen through inhibition of Rac and Rho/Rho-kinase. 血清素再摄取抑制剂通过抑制 Rac 和 Rho/Rho-激酶，抑制血小板在血小板生成素和胶原蛋白联合作用下的活化。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.231

Haruhiko Tokuda, Takamitsu Hori, Takashi Onuma, Yukiko Enomoto, Tomoaki Doi, Rie Matsushima-Nishiwaki, Shinobu Yamaguchi, Kumiko Tanabe, Takuya Omura, Shinji Ogura, Toru Iwama, Hiroki Iida, Osamu Kozawa

{"title":"Serotonin reuptake inhibitor suppresses the activation of human platelets by a combination of thrombopoietin and collagen through inhibition of Rac and Rho/Rho-kinase.","authors":"Haruhiko Tokuda, Takamitsu Hori, Takashi Onuma, Yukiko Enomoto, Tomoaki Doi, Rie Matsushima-Nishiwaki, Shinobu Yamaguchi, Kumiko Tanabe, Takuya Omura, Shinji Ogura, Toru Iwama, Hiroki Iida, Osamu Kozawa","doi":"10.2220/biomedres.45.231","DOIUrl":"https://doi.org/10.2220/biomedres.45.231","url":null,"abstract":"Tramadol and duloxetine, reuptake inhibitors of serotonin and noradrenaline, are widely used analgesics. Cytoplasmic serotonin in human platelets reportedly regulates the activity of low-molecular-weight GTP-binding proteins via serotonylation, leading to the modulation of platelet functions. We recently showed that the combination of thrombopoietin and collagen in the low doses synergistically induces human platelet activation via Rac and Rho/Rho-kinase. In the present study, we investigated the effects of tramadol and duloxetine on the synergistic effect, and the mechanism. Tramadol reduced the platelet aggregation and the release of PDGF-AB by the combination of thrombopoietin and collagen in the low doses. The aggregation and the release were also inhibited by duloxetine. Not reboxetine, a specific inhibitor of noradrenaline transporter, but fluvoxamine and sertraline, specific inhibitors of serotonin transporter suppressed the aggregation and the release. Tramadol, duloxetine, fluvoxamine and sertraline but not reboxetine attenuated the levels of GTP-Rac and GTP-Rho, and phospho-cofilin induced by the combination. Taken together, our results strongly suggest that tramadol and duloxetine, not as noradrenaline reuptake inhibitor but as serotonin reuptake inhibitor, suppress the activation of Rac and Rho/Rho-kinase elicited by the combination of subthreshold thrombopoietin and collagen, leading to the attenuation of human platelet activation.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 6","pages":"231-241"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indoxyl sulfate contributes to colorectal cancer cell proliferation and increased EGFR expression by activating AhR and Akt. 硫酸吲哚酯通过激活 AhR 和 Akt 促进结直肠癌细胞增殖和表皮生长因子受体表达增加。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.57

Yu Ichisaka, Shozo Yano, Kohji Nishimura, Toshimitsu Niwa, Hidehisa Shimizu

{"title":"Indoxyl sulfate contributes to colorectal cancer cell proliferation and increased EGFR expression by activating AhR and Akt.","authors":"Yu Ichisaka, Shozo Yano, Kohji Nishimura, Toshimitsu Niwa, Hidehisa Shimizu","doi":"10.2220/biomedres.45.57","DOIUrl":"10.2220/biomedres.45.57","url":null,"abstract":"Although patients with chronic kidney disease (CKD) have a higher risk of colorectal cancer (CRC) aggravation, the connection between these two diseases is not well understood. Recent studies have shown that both CKD and CRC aggravation are closely related to an increased abundance of indole-producing Fusobacterium nucleatum in the gut. The indole absorbed from the gut is eventually metabolized to indoxyl sulfate in the liver. Since indoxyl sulfate is involved not only in accelerating CKD progression but also in the initiation and development of its associated complications, the present study aimed to clarify whether indoxyl sulfate induces the proliferation of CRC cells. This study found that indoxyl sulfate induced the proliferation of CRC-derived HCT-116 cells by activating the aryl hydrocarbon receptor (AhR) and the proto-oncogene Akt. The AhR antagonist CH223191 and Akt inhibitor MK2206 suppressed indoxyl sulfate-induced proliferation of HCT-116 cells. We also found that indoxyl sulfate upregulated epidermal growth factor receptor (EGFR) expression, which is associated with poor prognosis of CRC, whereas CH223191 and MK2206 repressed EGFR expression. Furthermore, indoxyl sulfate increased the sensitivity of CRC cells to EGF by upregulating EGFR expression. These findings suggest that indoxyl sulfate may be an important link between CKD and CRC aggravation.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"57-66"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linalyl acetate exerts analgesic effects by inhibiting nociceptive TRPA1 in mice. 乙酸芳樟酯通过抑制小鼠的痛觉 TRPA1 发挥镇痛作用。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.125

Miho Hashimoto, Kenji Takahashi, Toshihiro Unno, Toshio Ohta

{"title":"Linalyl acetate exerts analgesic effects by inhibiting nociceptive TRPA1 in mice.","authors":"Miho Hashimoto, Kenji Takahashi, Toshihiro Unno, Toshio Ohta","doi":"10.2220/biomedres.45.125","DOIUrl":"10.2220/biomedres.45.125","url":null,"abstract":"Clary sage essential oil (CSEO) is utilized in perfumery, aromatherapy, and skincare. Linalyl acetate (LA), a primary component of CSEO, possesses sedative, anxiolytic, and analgesic properties. However, the mechanism of its analgesic action is not clearly understood. Transient receptor potential ankyrin 1 (TRPA1) channel, a non-selective cation channel, is mainly expressed in sensory neurons and serves as a sensor of various irritants. In this study, we investigated the effects of LA on TRPA1 channel using heterologous expression system and isolated sensory neurons. To detect channel activity, we employed Ca2+ imaging and the whole-cell patch-clamp technique. The analgesic action of LA was measured in a pain-related behavioral mouse model. In cells that heterologously expressed TRPA1, LA diminished [Ca2+]i and current responses to allylisothiocyanate (AITC) and carvacrol: exogenous TRPA1 agonists, and the inhibitory effects were more pronounced for the former than for the latter. Moreover, LA suppressed [Ca2+] i and current responses to PGJ2: an endogenous TRPA1 agonist. Similar inhibitory actions were observed in native TRPA1 channels expressed in mouse sensory neurons. Furthermore, LA diminished PGJ2-induced nociceptive behaviors in mice. These findings suggest that analgesic effects of LA exert through inhibition of nociceptive TRPA1, making it a potential candidate for novel analgesic development.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 3","pages":"125-133"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain. 不运动和运动干预对小鼠脑源性神经营养因子的影响：血清、骨骼肌和大脑中的动力学比较。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.163

Azusa Miki, Masahiro Aihara, Hikaru Kawaguchi, Noboru Hirose, Hiroki Hagiwara

{"title":"Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain.","authors":"Azusa Miki, Masahiro Aihara, Hikaru Kawaguchi, Noboru Hirose, Hiroki Hagiwara","doi":"10.2220/biomedres.45.163","DOIUrl":"10.2220/biomedres.45.163","url":null,"abstract":"Exercise training increases brain-derived neurotrophic factor (BDNF) expression and improves cognitive function. However, the dynamics of BDNF during inactivity and the effects of exercise intervention on BDNF levels have rarely been examined. Therefore, we aimed to examine changes in serum, skeletal muscle, and brain BDNF levels under these conditions. Mice were divided into control (Co), cast immobilization (CI), reloading (RL), and exercise (Ex) groups. Muscle atrophy was induced by cast immobilization for 2 weeks in the CI, RL, and Ex groups. After cast removal, the RL and Ex groups underwent regrounding and treadmill exercise, respectively, for 2 weeks. Serum, skeletal muscle, and brain BDNF levels showed a similar decreasing trend in the CI group, recovery in the RL group, and a further increase in the Ex group compared with those in the Co group. This indicates that BDNF levels change in parallel with the degree of activity. However, the magnitude of variation differed among the tissues in the order of serum > skeletal muscle > brain tissue. These results suggest that different mechanisms in different tissues regulate BDNF expression. BDNF could potentially act as an objective measure of the impact of both inactivity and exercise-based interventions.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 4","pages":"163-172"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0