Biomedical Research-tokyo最新文献_第3页

Propranolol and landiolol inhibit cell proliferation enhanced by noradrenaline in human lung adenocarcinoma cells. 普萘洛尔和兰地洛尔抑制人肺腺癌细胞在去甲肾上腺素作用下的增殖。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.253

Masahiro Tomihari, Masae Iwasaki, Masashi Ishikawa

{"title":"Propranolol and landiolol inhibit cell proliferation enhanced by noradrenaline in human lung adenocarcinoma cells.","authors":"Masahiro Tomihari, Masae Iwasaki, Masashi Ishikawa","doi":"10.2220/biomedres.45.253","DOIUrl":"https://doi.org/10.2220/biomedres.45.253","url":null,"abstract":"Previous clinical data have shown that perioperative β-blocker administration can improve lung cancer prognosis, possibly by blocking autonomic nervous system responses. This study aimed to investigate the anticancer mechanisms of the β-blockers propranolol and landiolol for human lung adenocarcinoma cells treated with noradrenaline. A549 human lung adenocarcinoma cells were exposed to each of the following alone or in combination for 2 h: medium only for naïve control; noradrenaline at a dose of 10 μmol/L; propranolol at 10 nmol/L; and landiolol at 1000 nmol/L. Cell proliferation was examined using a cell counting kit-8 assay and immunofluorescent staining of Ki67. qRT-PCR array was performed for Harvey rat sarcoma viral oncogene homolog (HRAS), transforming growth factor-beta receptor II (TGFBR2), and vascular endothelial growth factor A (VEGFA). Noradrenaline (N) showed enhanced cell proliferation compared to control, with higher Ki67 expression on immunostaining, higher HRAS and VEGFA expressions, and lower TGFBR2 expression in qRT-PCR, whereas N-propranolol and N-landiolol showed no significant changes. The present data indicated that perioperative administration of β-blockers might improve the post- operative prognosis of lung cancer via blockage of the adrenergic response.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 6","pages":"253-259"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenascin-X is increased with decreased expression of miR-378a-5p and miR-486-5p in mice fed a methionine-choline-deficient diet that induces hepatic fibrosis. 蛋氨酸-胆碱缺乏饮食会诱发小鼠肝纤维化，在小鼠体内，随着 miR-378a-5p 和 miR-486-5p 的表达减少，Tenascin-X 也会增加。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.67

Ken-Ichi Matsumoto, Takuma Higuchi, Mirei Umeki, Masafumi Ono, Shuji Sakamoto

{"title":"Tenascin-X is increased with decreased expression of miR-378a-5p and miR-486-5p in mice fed a methionine-choline-deficient diet that induces hepatic fibrosis.","authors":"Ken-Ichi Matsumoto, Takuma Higuchi, Mirei Umeki, Masafumi Ono, Shuji Sakamoto","doi":"10.2220/biomedres.45.67","DOIUrl":"10.2220/biomedres.45.67","url":null,"abstract":"We previously reported that tenascin-X (Tnxb) aggravates hepatic fibrosis in mice fed a high-fat and high-cholesterol diet with high levels of phosphorus and calcium (HFCD). In this study, we investigated Tnxb expression in livers with fibrosis caused by administration of a methionine-chorine-deficient (MCD) diet in mice. Whole transcriptome analysis showed that Tnxb was one of the genes with increased expression in livers of MCD diet-fed mice compared with that in livers of normal diet (ND)-fed mice. In microarray and subsequent microRNA (miRNA) network analyses, miR-378a-5p and miR-486-5p were identified in livers of MCD diet-fed mice as downregulated miRNAs, which have their predicted target sites in the 3' untranslated region of Tnxb mRNA and might suppress the translation of Tnxb mRNA. RT-qPCR analyses of livers of MCD diet-fed mice compared with livers of ND-fed mice verified the upregulation of Tnxb and fibrosis-triggering genes and conversely the downregulation of miR-378a-5p and miR-486-5p. Overexpression of miR-378a-5p and miR-486-5p resulted in decreased level not only of the FLAG-tagged fibrinogen-like domain of Tnxb protein (FLAG-mTNX-FG) but also of endogenous Tnxb protein in murine cultured cells. These results indicate that expression of Tnxb is regulated by miR-378a-5p and miR-486-5p in hepatic fibrosis following MCD diet feeding.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"67-76"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of 1,5-anhydro-D-fructose on reducing mental stress: a randomized, double-blind, placebo-controlled trial-a pilot study. 1,5-脱水-D-果糖对减轻精神压力的功效：随机、双盲、安慰剂对照试验--一项试点研究。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.209

Kiyoshi Kikuchi, Ryoji Kiyama, Kazuhiro Yoshinaga

{"title":"Efficacy of 1,5-anhydro-D-fructose on reducing mental stress: a randomized, double-blind, placebo-controlled trial-a pilot study.","authors":"Kiyoshi Kikuchi, Ryoji Kiyama, Kazuhiro Yoshinaga","doi":"10.2220/biomedres.45.209","DOIUrl":"10.2220/biomedres.45.209","url":null,"abstract":"Brain-derived neurotrophic factor (BDNF) plays an important role in mental stress. We have previously reported that 1,5-anhydro-D-fructose (1,5-AF) increases brain BDNF in vivo. The present randomized, controlled, double-blind study aimed to clinically evaluate the effects of 1,5-AF oral intake on mental stress in terms of three parameters: sleep, mood, and bowel issues. Healthy volunteers aged between 22 and 71 years (n = 24) were randomly assigned to receive 5.5 g of 1,5-AF or placebo orally, once daily for 4 weeks. Pre- and post-intervention, the subjects completed the Oguri-Shirakawa-Azumi Sleep Inventory, Middle-Aged and Aged Version (OSA-MA); Profile of Mood States, Second Edition (POMS2); and Constipation Assessment Scale (CAS) questionnaires. In the OSA-MA, both \"sleepiness on rising\" and \"sleep length\" were significantly improved after treatment with 1,5-AF compared with before treatment. Furthermore, in the POMS2, there was a clear tendency toward reduced \"Anger-Hostility\" in the 1,5-AF group after treatment, and in the CAS, there was a clear tendency toward reduced \"diarrhea or liquid stool\" in the 1,5-AF group after treatment. Together, our findings indicate that 1,5-AF has some effects on reducing post-intervention mental stress levels.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 5","pages":"209-216"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The characteristics and prevalence of patent foramen ovale in Japanese people: a study using material from forensic autopsies. 日本人卵圆孔未闭的特征和患病率：一项利用法医尸检材料进行的研究。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.135

Yoshiko Miyawaki, Masahito Hitosugi, Arisa Takeda, Marin Takaso, Mami Nakamura, Ikuo Kageyama

引用次数: 0

Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia. 内啡肽-1在大鼠颅内感觉神经节中的分布和抗痛觉功能

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.77

Shun Sato, Tadasu Sato, Takehiro Yajima, Daisuke Tachiya, Tetsu Takahashi, Shinnosuke Nogami, Masahiro Saito, Kensuke Yamauchi, Hiroyuki Ichikawa

{"title":"Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia.","authors":"Shun Sato, Tadasu Sato, Takehiro Yajima, Daisuke Tachiya, Tetsu Takahashi, Shinnosuke Nogami, Masahiro Saito, Kensuke Yamauchi, Hiroyuki Ichikawa","doi":"10.2220/biomedres.45.77","DOIUrl":"10.2220/biomedres.45.77","url":null,"abstract":"Distribution of endomorphin-1 (EM-1) was immunohistochemically investigated in the rat cranial sensory ganglia. Small to medium-sized neurons in the trigeminal (TG), petrosal (PG), and jugular ganglia (JG) expressed EM-1-immunoreactivity. However, EM-1-immunoreactive (-ir) neurons were infrequent in the nodose ganglion. In the brainstem, EM-1-ir varicose fibers were detected in the superficial layer of the medullary dorsal horn and the caudal part of the nucleus tractus solitarius. By trichrome immunofluorescence analysis, approximately 70% of EM-1-ir neurons were also immunoreactive for transient receptor potential vanilloid 1 (TRPV1) in all the examined ganglia. Additionally, 56.8% of EM1-ir TG neurons and approximately 30% of EM-1-ir PG and JG neurons showed calcitonin gene-related peptide (CGRP)-immunoreactivity. By a retrograde tracing method, several TG, PG, and JG neurons innervating the facial and external ear canal skin expressed EM-1-immunoreactivity. However, EM-1-ir neurons innervating the tooth pulp, circumvallate papilla, and pharynx were relatively rare. Thus, EM-1 expression and its coexistence with TRPV1 and CGRP in the cranial sensory neurons may depend on their various peripheral targets. EM1-ir neurons probably project to the superficial layer of the medullary dorsal horn and caudal part of the nucleus tractus solitarius. EM-1 may be involved in nociceptive transmission from the skin.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"77-89"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPRC5B (G protein-coupled receptor class C group 5 member B) suppresses glucose starvation-induced apoptosis in head-and-neck squamous cell carcinoma. GPRC5B (G蛋白偶联受体C类5组成员B)抑制葡萄糖饥饿诱导的头颈部鳞状细胞癌细胞凋亡。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2023-01-01 DOI: 10.2220/biomedres.44.1

Keisuke Kanamori, Shigeyuki Ozawa, Hiroshi Iwabuchi, Takeharu Ikoma, Kenji Suzuki, Kae Tanaka, Natsuko Yoshimura Sawai, Takahiro Abe, Yasumasa Kato, Ryu-Ichiro Hata, Masaru Kobayashi

{"title":"GPRC5B (G protein-coupled receptor class C group 5 member B) suppresses glucose starvation-induced apoptosis in head-and-neck squamous cell carcinoma.","authors":"Keisuke Kanamori, Shigeyuki Ozawa, Hiroshi Iwabuchi, Takeharu Ikoma, Kenji Suzuki, Kae Tanaka, Natsuko Yoshimura Sawai, Takahiro Abe, Yasumasa Kato, Ryu-Ichiro Hata, Masaru Kobayashi","doi":"10.2220/biomedres.44.1","DOIUrl":"https://doi.org/10.2220/biomedres.44.1","url":null,"abstract":"G protein-coupled receptor class C group 5 member B (GPRC5B) is involved in extracellular glucose sensing, glucose metabolism, and insulin resistance. Many cancers require glucose at high concentrations to survive and grow. We have investigated the association between tumour GPRC5B expression and the prognosis for patients with cancer, including head-and-neck squamous cell carcinoma (HNSCC), using data from The Human Protein Atlas. The 5-year survival rate was significantly reduced in patients with HNSCC, gastric, pancreatic, colorectal, and breast cancers if their tumours exhibited high levels of GPRC5B expression. The role of GPRC5B in glucose metabolism was assessed using six HNSCC cell lines with varying levels of GPRC5B expression. High levels of GPRC5B expression were found to favour rapid cell growth. The viability of an HNSCC cell line with normal and transfected GPRC5B expression was also assessed and no differences were observed under standard culture conditions. However, under glucose-deficient culture conditions, GPRC5B-overexpressing cells exhibited increased viability and reduced apoptosis. The results highlight the association between high GPRC5B expression and poor 5-year survival rates in patients with various cancers, including HNSCC. Furthermore, we have demonstrated that GPRC5B supports cancer cell survival under glucose-depleted conditions and could be a target molecule for cancer therapy.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"44 1","pages":"1-7"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9145538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mumefural prevents insulin resistance and amyloid-beta accumulation in the brain by improving lowered interstitial fluid pH in type 2 diabetes mellitus. Mumefural通过改善2型糖尿病间质液pH值降低，防止胰岛素抵抗和β淀粉样蛋白在脑内积聚。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2023-01-01 DOI: 10.2220/biomedres.44.17

Shigekuni Hosogi, Atsukazu Kuwahara, Yuko Kuwahara, Saori Tanaka, Chikao Shimamoto, Noriko Tagawa, Ikuo Kato, Kanji Yoshimoto, Wataru Aoi, Kazuyuki Takata, Hiroaki Miyazaki, Naomi Niisato, Yasuhiro Tsubo, Katsumi Yagi, Takashi Nakahari, Yoshinori Marunaka

{"title":"Mumefural prevents insulin resistance and amyloid-beta accumulation in the brain by improving lowered interstitial fluid pH in type 2 diabetes mellitus.","authors":"Shigekuni Hosogi, Atsukazu Kuwahara, Yuko Kuwahara, Saori Tanaka, Chikao Shimamoto, Noriko Tagawa, Ikuo Kato, Kanji Yoshimoto, Wataru Aoi, Kazuyuki Takata, Hiroaki Miyazaki, Naomi Niisato, Yasuhiro Tsubo, Katsumi Yagi, Takashi Nakahari, Yoshinori Marunaka","doi":"10.2220/biomedres.44.17","DOIUrl":"https://doi.org/10.2220/biomedres.44.17","url":null,"abstract":"The present study tried to clarify if mumefural would prevent hyperglycemia, one of the typical symptoms of type 2 diabetes mellitus (T2DM), since mumefural is an extract from Japanese apricots preventing hyperglycemia. To clarify if mumefural would prevent T2DM pathogenesis, we used Otsuka Long-Evans Tokushima fatty (OLETF) rats, T2DM model. Mumefural diminished hyperglycemia, HOMA-IR and plasma triglyceride concentration in OLETF rats under fasting conditions. In addition, mumefural elevated protein expression of sodium-coupled monocarboxylate transporter 1 (SMCT1) in the distal colon participating in absorption of weak organic acids, which behave as bases but not acids after absorption into the body. Mumefural also increased the interstitial fluid pH around the brain hippocampus lowered in OLETF rats compared with non-T2DM LETO rats used as control for OLETF rats. Amyloid-beta accumulation in the brain decreased in accordance with the pH elevation. On the one hand, mumefural didn't affect plasma concentrations of glucagon, GLP-1, GIP or PYY under fasting conditions. Taken together, these observations indicate that: 1) mumefural would be a useful functional food improving hyperglycemia, insulin resistance and the lowered interstitial fluid pH in T2DM; 2) the interstitial fluid pH would be one of key factors influencing the accumulation of amyloid-beta.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"44 1","pages":"17-29"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9130954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Sasa veitchii extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of miR-27b-5p. 黄芪提取物通过调节miR-27b-5p对苯妥英诱导的人唇间充质细胞增殖抑制有保护作用。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2023-01-01 DOI: 10.2220/biomedres.44.73

Yosuke Tsukiboshi, Aya Ogata, Azumi Noguchi, Yurie Mikami, Satoshi Yokota, Kenichi Ogata, Hiroki Yoshioka

{"title":"Sasa veitchii extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of miR-27b-5p.","authors":"Yosuke Tsukiboshi, Aya Ogata, Azumi Noguchi, Yurie Mikami, Satoshi Yokota, Kenichi Ogata, Hiroki Yoshioka","doi":"10.2220/biomedres.44.73","DOIUrl":"https://doi.org/10.2220/biomedres.44.73","url":null,"abstract":"A cleft lip, with or without a cleft palate, is a common birth defect caused by environmental factors or genetic mutations. Environmental factors, such as pharmaceutical exposure in pregnant women, are known to induce cleft lip, with or without cleft palate in the child. This study aimed to investigate the protective effect of Sasa veitchii extract (SE) on phenytoin-induced inhibition of cell proliferation in human lip mesenchymal cells (KD cells) and human embryonic palatal mesenchymal cells (HEPM cells). We demonstrated that cell proliferation was inhibited by phenytoin in a dose-dependent manner in both KD and HEPM cells. Co-treatment with SE restored phenytoin-induced toxicity in KD cells but did not protect HEPM cells against phenytoin-induced toxicity. Several microRNAs (miR-27b, miR-133b, miR-205, miR-497-5p, and miR-655-3p) is reported to associate with cell proliferation in KD cells. We measured the seven kinds of microRNAs (miR27b-3p, miR-27b-5p, miR-133b, miR-205-3p, miR-205-5p, miR-497-5p, and miR-655-3p) and found that SE suppressed miR-27b-5p induced by phenytoin in KD cells. Furthermore, co-treatment with SE enhanced the expression of miR-27b-5p downstream genes (PAX9, RARA, and SUMO1). These results suggest that SE protects phenytoin-induced cell proliferation inhibition by modulating miR-27b-5p.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"44 2","pages":"73-80"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9241032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Red foxtail millet upregulates brain-derived neurotrophic factor levels in vitro and in vivo. 红谷子在体外和体内上调脑源性神经营养因子水平。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2023-01-01 DOI: 10.2220/biomedres.44.97

Kensuke Nakajima, Hayato Tomohiro, Shigeru Oiso

{"title":"Red foxtail millet upregulates brain-derived neurotrophic factor levels in vitro and in vivo.","authors":"Kensuke Nakajima, Hayato Tomohiro, Shigeru Oiso","doi":"10.2220/biomedres.44.97","DOIUrl":"https://doi.org/10.2220/biomedres.44.97","url":null,"abstract":"Upregulation of the brain-derived neurotrophic factor (BDNF) in the brain can help in the prevention and treatment of depression. BDNF is synthesized in various peripheral tissues, as well as in the brain, and can reach the brain via the blood-brain barrier. Therefore, foods that upregulate peripheral BDNF levels may aid in depression management. We previously showed the BDNF-upregulating effect of white foxtail millet (WFM) using the human renal adenocarcinoma ACHN cell line, capable of producing and secreting BDNF. However, whether other varieties of foxtail millet can also upregulate BDNF is unclear. Herein, we examined the effects of red foxtail millet (RFM) on BDNF production in vitro and in vivo. RFM methanol extracts significantly increased BDNF levels in the culture medium of ACHN cells, and the levels were higher than those with WFMtreatment. Serum BDNF concentrations in rats fed a standard diet containing 20% RFM for 5 weeks were significantly higher than those in the control. Furthermore, the butanol fraction of the RFM methanol extract significantly increased BDNF levels in the culture medium of ACHN cells and upregulated BDNF mRNA expression in ACHN cells. Our results suggest that RFM has potential as a food material with BDNF-inducing activity.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"44 3","pages":"97-104"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9565505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of aquaporin 5 and glandular blood flow in the acetylcholine-induced secretion of saliva in rats. 水通道蛋白5和腺体血流在大鼠乙酰胆碱诱导的唾液分泌中的作用。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2023-01-01 DOI: 10.2220/biomedres.44.51

Mst Tahmina Akter, Akihiro Nezu, Tetsuya Akamatsu, Akihiko Tanimura

{"title":"Role of aquaporin 5 and glandular blood flow in the acetylcholine-induced secretion of saliva in rats.","authors":"Mst Tahmina Akter, Akihiro Nezu, Tetsuya Akamatsu, Akihiko Tanimura","doi":"10.2220/biomedres.44.51","DOIUrl":"https://doi.org/10.2220/biomedres.44.51","url":null,"abstract":"To clarify the role of the aquaporin 5 (AQP5) in salivary secretion, we evaluated acetylcholine (ACh)-induced secretion in Sprague-Dawley (SD) rats, rats expressing a low level of AQP5 protein (AQP5/low SD) which developed from SD rats, and Wistar/ST rats. The salivary secretion in AQP5/low SD rats in response to infusions of low-dose ACh (60-120 nmol/min) was 27-42% of that in SD rats. By contrast, Wistar/ST rats exhibited comparable secretion to that of SD rats in response to low-doses ACh, despite their low-level expression of AQP5. Experiments using spectrofluorometry and RT-PCR demonstrated no differences in the ACh-induced Ca2+ responses or the mRNA expression of muscarinic receptor, Cl- channel, or cotransporter between these strains. These findings imply that factors other than the function of salivary acinar cells regulates the secretion in response to weak stimuli. Monitoring of the hemodynamics in the submandibular gland revealed that low-doses ACh induced different patterns of the fluctuations in the blood flow in these strains. The blood flow decreased below the resting level in AQP5/low SD rats, but remained mostly above the resting level in Wistar/ST rats. The present study reveals that the contribution of AQP5-dependent transport of water is altered by stimulus intensity and blood flow.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"44 2","pages":"51-63"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10334429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0