Biomedical Research-tokyo最新文献_第3页

Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain. 不运动和运动干预对小鼠脑源性神经营养因子的影响：血清、骨骼肌和大脑中的动力学比较。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.163

Azusa Miki, Masahiro Aihara, Hikaru Kawaguchi, Noboru Hirose, Hiroki Hagiwara

{"title":"Effects of inactivity and exercise intervention on brain-derived neurotrophic factor in mice: Comparison of kinetics in serum, skeletal muscle, and brain.","authors":"Azusa Miki, Masahiro Aihara, Hikaru Kawaguchi, Noboru Hirose, Hiroki Hagiwara","doi":"10.2220/biomedres.45.163","DOIUrl":"10.2220/biomedres.45.163","url":null,"abstract":"Exercise training increases brain-derived neurotrophic factor (BDNF) expression and improves cognitive function. However, the dynamics of BDNF during inactivity and the effects of exercise intervention on BDNF levels have rarely been examined. Therefore, we aimed to examine changes in serum, skeletal muscle, and brain BDNF levels under these conditions. Mice were divided into control (Co), cast immobilization (CI), reloading (RL), and exercise (Ex) groups. Muscle atrophy was induced by cast immobilization for 2 weeks in the CI, RL, and Ex groups. After cast removal, the RL and Ex groups underwent regrounding and treadmill exercise, respectively, for 2 weeks. Serum, skeletal muscle, and brain BDNF levels showed a similar decreasing trend in the CI group, recovery in the RL group, and a further increase in the Ex group compared with those in the Co group. This indicates that BDNF levels change in parallel with the degree of activity. However, the magnitude of variation differed among the tissues in the order of serum > skeletal muscle > brain tissue. These results suggest that different mechanisms in different tissues regulate BDNF expression. BDNF could potentially act as an objective measure of the impact of both inactivity and exercise-based interventions.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 4","pages":"163-172"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of skeletal muscle differentiation by calciprotein particles in human primary myoblasts. 钙蛋白颗粒对人类原发性肌母细胞骨骼肌分化的抑制作用

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.173

Shohei Kohno, Eisuke Uno, Kazuto Goishi, Davood Kharaghani, Kenta Uchibe, Ryuji Terayama

引用次数: 0

Epigenetic modification of histone acetylation in the sensorimotor cortex after intracerebral hemorrhage. 脑出血后感觉运动皮层组蛋白乙酰化的表观遗传修饰

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.1

Taichi Nishio, Takahiro Inoue, Yasuyuki Takamatsu, Taiga Mishima, Hana Takamura, Kiho Soma, Yuki Kondo, Misato Okamura, Ryo Ikegami, Hiroshi Maejima

{"title":"Epigenetic modification of histone acetylation in the sensorimotor cortex after intracerebral hemorrhage.","authors":"Taichi Nishio, Takahiro Inoue, Yasuyuki Takamatsu, Taiga Mishima, Hana Takamura, Kiho Soma, Yuki Kondo, Misato Okamura, Ryo Ikegami, Hiroshi Maejima","doi":"10.2220/biomedres.45.1","DOIUrl":"10.2220/biomedres.45.1","url":null,"abstract":"Epigenetic regulation is involved in post-stroke neuroplasticity. We investigated the effects of intracerebral hemorrhage (ICH) on histone acetylation and gene expression related to neuronal plasticity in the bilateral sensorimotor cortices, which may affect post-stroke sensorimotor function. Wistar rats were randomly divided into the SHAM and ICH groups. We performed ICH surgery stereotaxically based on the microinjection of a collagenase solution in the ICH group. Foot fault and cylinder tests were performed to evaluate motor functions at 4-time points, including pre-ICH surgery. The amount of acetyl histones and the mRNA expression of neurotrophic factors crucial to neuroplasticity in the bilateral sensorimotor cortices were analyzed approximately 2 weeks after ICH surgery. Sensorimotor functions of the ICH group were inferior to those of the SHAM group during 2 weeks post-ICH. ICH increased the acetylation of histone H3 and H4 over the sham level in the ipsilateral and contralateral cortices. ICH increased the mRNA expression of IGF-1, but decreased the expression of BDNF compared with the sham level in the ipsilateral cortex. The present study suggests that histone acetylation levels are enhanced in bilateral sensorimotor cortices after ICH, presenting an altered epigenetic platform for gene expressions related to neuronal plasticity.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 1","pages":"1-11"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139701813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin protects human coronary artery endothelial cells against hypoxia/reoxygenation-induced mitochondrial apoptosis via the Nrf2/HO-1 axis. 槲皮素通过Nrf2/HO-1轴保护人冠状动脉内皮细胞免受缺氧/复氧诱导的线粒体凋亡。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.197

Jiejin Song, Shuang Li, Boyong Zhang, Jiao Wu, Aiqin Zhong

{"title":"Quercetin protects human coronary artery endothelial cells against hypoxia/reoxygenation-induced mitochondrial apoptosis via the Nrf2/HO-1 axis.","authors":"Jiejin Song, Shuang Li, Boyong Zhang, Jiao Wu, Aiqin Zhong","doi":"10.2220/biomedres.45.197","DOIUrl":"10.2220/biomedres.45.197","url":null,"abstract":"Our study explored the therapeutic effect and the mechanism of quercetin against hypoxia/reoxygenation (H/R)-induced injury in human coronary artery endothelial cells (CAECs). Quercetin was selected as a potential component for the BuShenKangShuaiPian formula (BSKSP) treatment via the Network pharmacology analysis. Cell viability and reactive oxygen species (ROS) production were measured by CCK8 assay and immunofluorescence, respectively. The expression of Bax, Bcl-2, Cle-caspase-3, cytochrome c (Cyt-C), NF-E2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) protein was quantified by western blotting. The superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) activity, mtDNA copy number, and ATP production were measured via corresponding kits. Quercetin was selected from the BSKSP for its high degree value (Degree value: 22). Besides, quercetin protected CAECs against H/R-induced cytotoxicity and apoptosis. The H/R-induced increased ROS level, ATP production, Cyt-C release, and decreased mtDNA copy number were removed by the quercetin. Moreover, quercetin upregulated the Nrf2/ HO-1 axis, SOD, and CAT activity, and downregulated MDA levels in H/R treated CAECs, while knockdown Nrf2 reversed the protection of quercetin against H/R-induced oxidative stress, mitochondrial damage, and apoptosis. Quercetin protects CAECs against H/R-induced mitochondrial apoptosis via the Nrf2/HO-1 axis, which innovatively suggests the therapeutic potential of quercetin for coronary heart disease (CHD) treatment.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 5","pages":"197-207"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using hemoglobin vesicles to treat operative hemorrhagic shock after pneu- monectomy in dog models: an experimental study. 在狗模型中使用血红蛋白囊治疗气囊切除术后的手术失血性休克：一项实验研究。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.91

Kei Nakano, Mitsutomo Kohno, Hiroto Onozawa, Ryo Hashimoto, Kana Oiwa, Ryota Masuda, Masatoshi Yamaguchi, Tai Hato, Masazumi Watanabe, Hirohisa Horinouchi, Hiromi Sakai, Koichi Kobayashi, Masayuki Iwazaki

{"title":"Using hemoglobin vesicles to treat operative hemorrhagic shock after pneu- monectomy in dog models: an experimental study.","authors":"Kei Nakano, Mitsutomo Kohno, Hiroto Onozawa, Ryo Hashimoto, Kana Oiwa, Ryota Masuda, Masatoshi Yamaguchi, Tai Hato, Masazumi Watanabe, Hirohisa Horinouchi, Hiromi Sakai, Koichi Kobayashi, Masayuki Iwazaki","doi":"10.2220/biomedres.45.91","DOIUrl":"10.2220/biomedres.45.91","url":null,"abstract":"Hemoglobin vesicles (HbVs), considered as red blood cell substitutes, are liposomes encapsulating purified hemoglobin, with a phospholipid bilayer membrane (diameter: 250 nm; P50, 28 Torr). In this study, we aimed to investigate HbV function during hemorrhagic shock in lung resection and analyze the details of oxygen delivery. Left pneumonectomy was performed in dogs under mechanical ventilation, followed by rapid exsanguination of approximately 30% of the total circulating blood volume, which led to shock, reducing the mean arterial pressure (MAP) by approximately 60% of baseline. Subsequently, either 5% human serum albumin (HSA) or HbVs suspended in 5% HSA were infused for resuscitation. The MAP only recovered to 75% of baseline after HSA administration, but fully recovered (100%) after HbV administration, with significant differences between the groups (P < 0.005). Oxygen delivery was restored in the HbV group and was significantly higher than that in the HSA group (P < 0.0001). The infusion of HbVs dispersed in a 5% HSA solution compensated for the rapid loss of approximately 30% of the total circulating blood volume in a dog pneumonectomy model, even with impaired lung function. Thus, HbVs can be used for resuscitation from hemorrhagic shock during thoracic surgery.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"91-101"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Propranolol and landiolol inhibit cell proliferation enhanced by noradrenaline in human lung adenocarcinoma cells. 普萘洛尔和兰地洛尔抑制人肺腺癌细胞在去甲肾上腺素作用下的增殖。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.253

Masahiro Tomihari, Masae Iwasaki, Masashi Ishikawa

{"title":"Propranolol and landiolol inhibit cell proliferation enhanced by noradrenaline in human lung adenocarcinoma cells.","authors":"Masahiro Tomihari, Masae Iwasaki, Masashi Ishikawa","doi":"10.2220/biomedres.45.253","DOIUrl":"https://doi.org/10.2220/biomedres.45.253","url":null,"abstract":"Previous clinical data have shown that perioperative β-blocker administration can improve lung cancer prognosis, possibly by blocking autonomic nervous system responses. This study aimed to investigate the anticancer mechanisms of the β-blockers propranolol and landiolol for human lung adenocarcinoma cells treated with noradrenaline. A549 human lung adenocarcinoma cells were exposed to each of the following alone or in combination for 2 h: medium only for naïve control; noradrenaline at a dose of 10 μmol/L; propranolol at 10 nmol/L; and landiolol at 1000 nmol/L. Cell proliferation was examined using a cell counting kit-8 assay and immunofluorescent staining of Ki67. qRT-PCR array was performed for Harvey rat sarcoma viral oncogene homolog (HRAS), transforming growth factor-beta receptor II (TGFBR2), and vascular endothelial growth factor A (VEGFA). Noradrenaline (N) showed enhanced cell proliferation compared to control, with higher Ki67 expression on immunostaining, higher HRAS and VEGFA expressions, and lower TGFBR2 expression in qRT-PCR, whereas N-propranolol and N-landiolol showed no significant changes. The present data indicated that perioperative administration of β-blockers might improve the post- operative prognosis of lung cancer via blockage of the adrenergic response.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 6","pages":"253-259"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenascin-X is increased with decreased expression of miR-378a-5p and miR-486-5p in mice fed a methionine-choline-deficient diet that induces hepatic fibrosis. 蛋氨酸-胆碱缺乏饮食会诱发小鼠肝纤维化，在小鼠体内，随着 miR-378a-5p 和 miR-486-5p 的表达减少，Tenascin-X 也会增加。

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.67

Ken-Ichi Matsumoto, Takuma Higuchi, Mirei Umeki, Masafumi Ono, Shuji Sakamoto

{"title":"Tenascin-X is increased with decreased expression of miR-378a-5p and miR-486-5p in mice fed a methionine-choline-deficient diet that induces hepatic fibrosis.","authors":"Ken-Ichi Matsumoto, Takuma Higuchi, Mirei Umeki, Masafumi Ono, Shuji Sakamoto","doi":"10.2220/biomedres.45.67","DOIUrl":"10.2220/biomedres.45.67","url":null,"abstract":"We previously reported that tenascin-X (Tnxb) aggravates hepatic fibrosis in mice fed a high-fat and high-cholesterol diet with high levels of phosphorus and calcium (HFCD). In this study, we investigated Tnxb expression in livers with fibrosis caused by administration of a methionine-chorine-deficient (MCD) diet in mice. Whole transcriptome analysis showed that Tnxb was one of the genes with increased expression in livers of MCD diet-fed mice compared with that in livers of normal diet (ND)-fed mice. In microarray and subsequent microRNA (miRNA) network analyses, miR-378a-5p and miR-486-5p were identified in livers of MCD diet-fed mice as downregulated miRNAs, which have their predicted target sites in the 3' untranslated region of Tnxb mRNA and might suppress the translation of Tnxb mRNA. RT-qPCR analyses of livers of MCD diet-fed mice compared with livers of ND-fed mice verified the upregulation of Tnxb and fibrosis-triggering genes and conversely the downregulation of miR-378a-5p and miR-486-5p. Overexpression of miR-378a-5p and miR-486-5p resulted in decreased level not only of the FLAG-tagged fibrinogen-like domain of Tnxb protein (FLAG-mTNX-FG) but also of endogenous Tnxb protein in murine cultured cells. These results indicate that expression of Tnxb is regulated by miR-378a-5p and miR-486-5p in hepatic fibrosis following MCD diet feeding.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"67-76"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of 1,5-anhydro-D-fructose on reducing mental stress: a randomized, double-blind, placebo-controlled trial-a pilot study. 1,5-脱水-D-果糖对减轻精神压力的功效：随机、双盲、安慰剂对照试验--一项试点研究。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.209

Kiyoshi Kikuchi, Ryoji Kiyama, Kazuhiro Yoshinaga

{"title":"Efficacy of 1,5-anhydro-D-fructose on reducing mental stress: a randomized, double-blind, placebo-controlled trial-a pilot study.","authors":"Kiyoshi Kikuchi, Ryoji Kiyama, Kazuhiro Yoshinaga","doi":"10.2220/biomedres.45.209","DOIUrl":"10.2220/biomedres.45.209","url":null,"abstract":"Brain-derived neurotrophic factor (BDNF) plays an important role in mental stress. We have previously reported that 1,5-anhydro-D-fructose (1,5-AF) increases brain BDNF in vivo. The present randomized, controlled, double-blind study aimed to clinically evaluate the effects of 1,5-AF oral intake on mental stress in terms of three parameters: sleep, mood, and bowel issues. Healthy volunteers aged between 22 and 71 years (n = 24) were randomly assigned to receive 5.5 g of 1,5-AF or placebo orally, once daily for 4 weeks. Pre- and post-intervention, the subjects completed the Oguri-Shirakawa-Azumi Sleep Inventory, Middle-Aged and Aged Version (OSA-MA); Profile of Mood States, Second Edition (POMS2); and Constipation Assessment Scale (CAS) questionnaires. In the OSA-MA, both \"sleepiness on rising\" and \"sleep length\" were significantly improved after treatment with 1,5-AF compared with before treatment. Furthermore, in the POMS2, there was a clear tendency toward reduced \"Anger-Hostility\" in the 1,5-AF group after treatment, and in the CAS, there was a clear tendency toward reduced \"diarrhea or liquid stool\" in the 1,5-AF group after treatment. Together, our findings indicate that 1,5-AF has some effects on reducing post-intervention mental stress levels.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 5","pages":"209-216"},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The characteristics and prevalence of patent foramen ovale in Japanese people: a study using material from forensic autopsies. 日本人卵圆孔未闭的特征和患病率：一项利用法医尸检材料进行的研究。

IF 1.3 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.135

Yoshiko Miyawaki, Masahito Hitosugi, Arisa Takeda, Marin Takaso, Mami Nakamura, Ikuo Kageyama

引用次数: 0

Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia. 内啡肽-1在大鼠颅内感觉神经节中的分布和抗痛觉功能

IF 1.2 4区医学

Biomedical Research-tokyo Pub Date : 2024-01-01 DOI: 10.2220/biomedres.45.77

Shun Sato, Tadasu Sato, Takehiro Yajima, Daisuke Tachiya, Tetsu Takahashi, Shinnosuke Nogami, Masahiro Saito, Kensuke Yamauchi, Hiroyuki Ichikawa

{"title":"Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia.","authors":"Shun Sato, Tadasu Sato, Takehiro Yajima, Daisuke Tachiya, Tetsu Takahashi, Shinnosuke Nogami, Masahiro Saito, Kensuke Yamauchi, Hiroyuki Ichikawa","doi":"10.2220/biomedres.45.77","DOIUrl":"10.2220/biomedres.45.77","url":null,"abstract":"Distribution of endomorphin-1 (EM-1) was immunohistochemically investigated in the rat cranial sensory ganglia. Small to medium-sized neurons in the trigeminal (TG), petrosal (PG), and jugular ganglia (JG) expressed EM-1-immunoreactivity. However, EM-1-immunoreactive (-ir) neurons were infrequent in the nodose ganglion. In the brainstem, EM-1-ir varicose fibers were detected in the superficial layer of the medullary dorsal horn and the caudal part of the nucleus tractus solitarius. By trichrome immunofluorescence analysis, approximately 70% of EM-1-ir neurons were also immunoreactive for transient receptor potential vanilloid 1 (TRPV1) in all the examined ganglia. Additionally, 56.8% of EM1-ir TG neurons and approximately 30% of EM-1-ir PG and JG neurons showed calcitonin gene-related peptide (CGRP)-immunoreactivity. By a retrograde tracing method, several TG, PG, and JG neurons innervating the facial and external ear canal skin expressed EM-1-immunoreactivity. However, EM-1-ir neurons innervating the tooth pulp, circumvallate papilla, and pharynx were relatively rare. Thus, EM-1 expression and its coexistence with TRPV1 and CGRP in the cranial sensory neurons may depend on their various peripheral targets. EM1-ir neurons probably project to the superficial layer of the medullary dorsal horn and caudal part of the nucleus tractus solitarius. EM-1 may be involved in nociceptive transmission from the skin.","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":"45 2","pages":"77-89"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140331523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0