脑出血后感觉运动皮层组蛋白乙酰化的表观遗传修饰

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Taichi Nishio, Takahiro Inoue, Yasuyuki Takamatsu, Taiga Mishima, Hana Takamura, Kiho Soma, Yuki Kondo, Misato Okamura, Ryo Ikegami, Hiroshi Maejima
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引用次数: 0

摘要

表观遗传调控参与了中风后的神经可塑性。我们研究了脑内出血(ICH)对双侧感觉运动皮层神经元可塑性相关组蛋白乙酰化和基因表达的影响,这可能会影响中风后的感觉运动功能。将 Wistar 大鼠随机分为 SHAM 组和 ICH 组。我们在ICH组大鼠体内注射胶原酶溶液的基础上进行了立体定向ICH手术。我们在4个时间点(包括ICH手术前)对大鼠的运动功能进行了足部错位和圆柱体测试。ICH 手术后约 2 周,分析了双侧感觉运动皮层中乙酰组蛋白的含量和对神经可塑性至关重要的神经营养因子的 mRNA 表达。在ICH术后2周内,ICH组的感觉运动功能低于SHAM组。ICH 使同侧和对侧大脑皮层组蛋白 H3 和 H4 的乙酰化水平高于假性水平。与假体水平相比,ICH 增加了同侧大脑皮层 IGF-1 的 mRNA 表达,但降低了 BDNF 的表达。本研究表明,ICH后双侧感觉运动皮层的组蛋白乙酰化水平升高,为与神经元可塑性相关的基因表达提供了一个改变的表观遗传平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epigenetic modification of histone acetylation in the sensorimotor cortex after intracerebral hemorrhage.

Epigenetic regulation is involved in post-stroke neuroplasticity. We investigated the effects of intracerebral hemorrhage (ICH) on histone acetylation and gene expression related to neuronal plasticity in the bilateral sensorimotor cortices, which may affect post-stroke sensorimotor function. Wistar rats were randomly divided into the SHAM and ICH groups. We performed ICH surgery stereotaxically based on the microinjection of a collagenase solution in the ICH group. Foot fault and cylinder tests were performed to evaluate motor functions at 4-time points, including pre-ICH surgery. The amount of acetyl histones and the mRNA expression of neurotrophic factors crucial to neuroplasticity in the bilateral sensorimotor cortices were analyzed approximately 2 weeks after ICH surgery. Sensorimotor functions of the ICH group were inferior to those of the SHAM group during 2 weeks post-ICH. ICH increased the acetylation of histone H3 and H4 over the sham level in the ipsilateral and contralateral cortices. ICH increased the mRNA expression of IGF-1, but decreased the expression of BDNF compared with the sham level in the ipsilateral cortex. The present study suggests that histone acetylation levels are enhanced in bilateral sensorimotor cortices after ICH, presenting an altered epigenetic platform for gene expressions related to neuronal plasticity.

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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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