在狗模型中使用血红蛋白囊治疗气囊切除术后的手术失血性休克:一项实验研究。

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Kei Nakano, Mitsutomo Kohno, Hiroto Onozawa, Ryo Hashimoto, Kana Oiwa, Ryota Masuda, Masatoshi Yamaguchi, Tai Hato, Masazumi Watanabe, Hirohisa Horinouchi, Hiromi Sakai, Koichi Kobayashi, Masayuki Iwazaki
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引用次数: 0

摘要

血红蛋白小泡(HbVs)被认为是红细胞的替代物,它是包裹着纯化血红蛋白的脂质体,具有磷脂双层膜(直径:250 nm;P50,28 Torr)。在这项研究中,我们旨在研究 HbV 在肺切除术中失血性休克期间的功能,并分析氧输送的细节。在机械通气下对狗进行左肺切除术,然后快速放血约30%的总循环血量,从而导致休克,使平均动脉压(MAP)降低约基线的60%。随后,注入 5%的人血清白蛋白(HSA)或悬浮在 5%的 HSA 中的 HbVs 进行复苏。给予 HSA 后,MAP 仅恢复到基线的 75%,而给予 HbV 后则完全恢复(100%),组间差异显著(P < 0.005)。HbV 组的供氧量得到恢复,且明显高于 HSA 组(P < 0.0001)。在狗肺切除术模型中,即使在肺功能受损的情况下,输注分散在 5% HSA 溶液中的 HbVs 也能弥补总循环血量约 30% 的快速损失。因此,HbVs 可用于胸外科手术中失血性休克的复苏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using hemoglobin vesicles to treat operative hemorrhagic shock after pneu- monectomy in dog models: an experimental study.

Hemoglobin vesicles (HbVs), considered as red blood cell substitutes, are liposomes encapsulating purified hemoglobin, with a phospholipid bilayer membrane (diameter: 250 nm; P50, 28 Torr). In this study, we aimed to investigate HbV function during hemorrhagic shock in lung resection and analyze the details of oxygen delivery. Left pneumonectomy was performed in dogs under mechanical ventilation, followed by rapid exsanguination of approximately 30% of the total circulating blood volume, which led to shock, reducing the mean arterial pressure (MAP) by approximately 60% of baseline. Subsequently, either 5% human serum albumin (HSA) or HbVs suspended in 5% HSA were infused for resuscitation. The MAP only recovered to 75% of baseline after HSA administration, but fully recovered (100%) after HbV administration, with significant differences between the groups (P < 0.005). Oxygen delivery was restored in the HbV group and was significantly higher than that in the HSA group (P < 0.0001). The infusion of HbVs dispersed in a 5% HSA solution compensated for the rapid loss of approximately 30% of the total circulating blood volume in a dog pneumonectomy model, even with impaired lung function. Thus, HbVs can be used for resuscitation from hemorrhagic shock during thoracic surgery.

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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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