BMC Complementary Medicine and Therapies最新文献

{"title":"Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin.","authors":"Nihal Turkmen Alemdar, Selim Demir, Esin Yulug, Ali Kulaber, Elif Ayazoglu Demir, Nadire Sevdenur Erdogan, Ahmet Mentese, Yuksel Aliyazicioglu","doi":"10.1186/s12906-025-04944-5","DOIUrl":"10.1186/s12906-025-04944-5","url":null,"abstract":"<p><strong>Background: </strong>Acetamiprid (ACP) is a novel chloronicotinyl insecticide that has been extensively utilized in agricultural, domestic, and public health contexts for nearly two decades. However, its potential to induce organ damage, including reproductive toxicity in mammals, has emerged as a significant concern. Morin is a naturally occurring flavonol that has gained prominence as a food supplement in recent years due to its antioxidant and anti-inflammatory properties. The objective of this study was to evaluate the protective effect of morin against testicular damage in mice subjected to ACP exposure.</p><p><strong>Methods: </strong>Thirty male Balb/c mice were randomly assigned to one of five groups, with the following treatment allocations: control, ACP (20 mg/kg), ACP + morin (15 and 30 mg/kg), and only morin (30 mg/kg). ACP and morin applications were conducted orally over a period of 14 days. Hormonal analyses were conducted on serum samples obtained from the mice, while biochemical and histological evaluations were performed on testicular samples.</p><p><strong>Results: </strong>The biochemical results demonstrated that ACP elevated oxidative stress, inflammation, and ER stress in testicular tissue by inhibiting the Nrf2 pathway, a finding that was corroborated by histopathological analyses. However, morin treatments eliminated ACP-induced Nrf2 inhibition and to activate antioxidant and anti-inflammatory mechanisms. These findings were also corroborated by the restoration of serum testosterone and inhibin B levels and the diminution of histopathological lesions.</p><p><strong>Conclusions: </strong>Overall, the findings indicated that morin may have potential protective properties against ACP-associated reproductive toxicity, however, further research is required to determine the detailed molecular mechanisms.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"203"},"PeriodicalIF":3.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of herbal medicine consumption and regulatory compliance in some selected districts of the Bono region, Ghana.","authors":"George Owusu, Meshack Antwi-Adjei, Jones Ofori-Amoah, Ransford Mawuli Tuekpe, Adjei Emmanuel Mainoo, Dorothy Kodua, Anatua Abigail, Oppong Millicent","doi":"10.1186/s12906-025-04953-4","DOIUrl":"10.1186/s12906-025-04953-4","url":null,"abstract":"<p><strong>Background: </strong>Though herbal medicine remains a vital component of healthcare in the Bono region of Ghana, knowledge of its prevalence, factors influencing the choice of herbal remedies, and compliance with quality control standards among the herbal medicine practitioners in the region is lacking. This situation adversely affects health outcomes, regulatory standards, and public trust in herbal medicines in the region.</p><p><strong>Aim: </strong>This study aimed at assessing the prevalence of herbal medicine use, factors that dictate the choice of herbal medicine, and adherence to quality control practices in the Bono region of Ghana.</p><p><strong>Method: </strong>A cross-sectional study with 504 recruited herbal medicine consumers and 98 practitioners, selected across four districts of the Bono region, was used. Paper-pencil questionnaires were used to obtain respondents' sociodemographic data. The chi-square (χ2) test was used to assess associated factors, while logistic regression analysis was used to determine the factors associated with herbal medicine use.</p><p><strong>Results: </strong>It was revealed that 92.7% of the respondents had used herbal medicine within the past 24 months. Old age (P < 0.001), farming (P < 0.001), self-employment (P < 0.004), unemployment (P < 0.049), and no formal education (P < 0.044) were associated with herbal medicine usage. Of the 98 practitioners, 44.9% had active FDA licenses, and 59.2% had undergone formal training in quality control practices at recognized institutions. Of the 73 herbal products identified, 46.5% had no FDA registration certification, 42.5% had no information on unwanted effects and contraindications, and 26% had no expiry dates.</p><p><strong>Conclusion: </strong>The study highlighted a widespread use of herbal medicine and a significant regulatory compliance gap in the Bono region. As herbal medicine remains an essential remedy in this area, commitments from the government, regulatory bodies, practitioners, and the general public are required to improve regulatory adherence to safeguard public health.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"202"},"PeriodicalIF":3.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal plants traditionally used for management of malaria in rural communities of Uganda.","authors":"Lydia Bunalema, Moses Ocan, Francis Williams Ojara, Sam Nsobya, Charles Okot Odongo, Gordon Odia, Aloysious Lubega","doi":"10.1186/s12906-025-04946-3","DOIUrl":"10.1186/s12906-025-04946-3","url":null,"abstract":"<p><strong>Background: </strong>Malaria remains a major cause of morbidity and mortality especially in sub-Saharan Africa. Whereas herbal medicines have long been used for disease remedy in many African communities, there is limited evidence on the extent of use, their safety, and efficacy. This study, sought to identify herbal medicinal plants used by communities in low and high malaria transmission settings in Uganda for managing of malaria.</p><p><strong>Method: </strong>An Ethnobotanical survey was conducted across four geographical regions purposively selected to represent moderate-to-high (Apac, Arua and Tororo districts) and low (Kabale district) malaria transmission settings. One-hundred and two (102) traditional medicine practitioners (TMPs) in Ugandan local communities were included in the study. A checklist was used to collect data and covered the following areas; knowledge on malaria transmission, malaria symptoms, diagnosis, medicinal plants used, preparations, preservation methods and doses. Data was analyzed in MS Excel^®. Consensus factor, use value metrics and frequencies were calculated.</p><p><strong>Results: </strong>Ninety-seven plant species distributed across 45 families were mentioned by TMPs in management of malaria in Ugandan communities. Plant family Asteraceae, 15.5% (15/97) had the highest distribution of plants reported by TMPs. Vernonia amygdalina Delile, Aloe vera Burm. F., Artemisia annua L., Vernonia grantii Oliv. and Justicia betonica L. were the most mentioned, with use values of 0.4, 0.3, 0.2, 0.15 and 0.14 respectively. Leaves 64% and root barks 18% were the most harvested plant parts while decoctions (54%) and infusions 26% were the most common methods of preparing herbal products for individuals with malaria. Medicines were stored as dry powders for extended periods although some were prepared as fresh plants. Nearly all medicinal preparations were administered orally with varying dosage (5 ml-500 ml*3times a day) recommendations. Treatment duration varied between 3 and 7 days among practitioners. TMPs mentioned that malaria is transmitted by mosquitoes while others, said poor hygiene, stagnant water and body contact.</p><p><strong>Conclusion: </strong>A diverse number of plant species, use and preparation methods are documented in this study as a way of preserving traditional knowledge in Uganda. Vernonia amygdalina Del., Aloe vera (L) Burm. f, Vernonia grantii Oliv. and Justicia betonica L. were identified as important plant species that can be further studied to validate their safety, antiplasmodial and active bioactive phytochemicals that can provide novel lead compounds for malaria treatment. These plant species can also be conserved through cultivation for sustainable use.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"201"},"PeriodicalIF":3.3,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative investigation on Hippocratea africana root: insights from cardio-protective, anti-oxidative stress activities, isolation, GC/MS, and pharmacological significance profiling.","authors":"Edet Effiong Asanga, Kufre Uyo Noah, Jude Efiom Okokon, Chinedum Martins Ekeleme, Imaobong Etima Udoh, Augustine Bassey, Martin Osita Anagboso, Aniekeme Ndisa Inyang","doi":"10.1186/s12906-025-04941-8","DOIUrl":"10.1186/s12906-025-04941-8","url":null,"abstract":"<p><strong>Background: </strong>Hippocratea africana root is scientifically used in cardiotoxicity treatment; therefore, this study was designed to validate this claims through the evaluation of its cardioprotective activity, isolation, and characterization of its constituents, as well as in silico profiling of these ligands against antioxidant enzymes.</p><p><strong>Methods: </strong>Standard protocols were followed in the extraction, fractionation, isolation, characterization, evaluation of cardio-protective activity (marker enzymes, oxidative stress markers, and histological section), retrieval of target proteins (SOD, CAT, GPx, and GSH), ADMET, and docking studies. Column and thin layer chromatography as well as GC/MS aided the isolation and characterization of compounds; SWISSADME and ADMET lab 2 enhanced the evaluation of pharmacokinetic properties; PyRx for docking analysis; Biovia discovery studio and PyMol software for 2D and 3D visualization of the ligand-protein interactions.</p><p><strong>Results: </strong>The root extract administration significantly (p < 0.05) reduced the serum levels of CK-MB, LDH, and troponin I that were elevated after doxorubicin administration; however, the levels of GSH, GST, SOD, GPx, and CAT that were decreased after doxorubicin administration were significantly (p < 0.05) elevated whereas the raised MDA level was reduced after treatment with the extract and fractions of the plant. Also, the histological sections in the extract-treated rats showed reductions in pathological features as compared with the negative control group. Moreover, the chemical pathological changes were consistent with histopathological observations suggesting marked cardio-protective potentials. Furthermore, the chromatographic analyses yielded bulked fractions {D3 (11 mg), E6 (25 mg), and E8 (21 mg)} and their GC/MS analyses revealed dihydroartemisinin; retinoic acid, methyl ester; α-thujene; α-terpinolene; 9,12,15-octadecatrienal; α-terpineol etc. with already reported antioxidant activities. These ligands (dihydroartemisinin; retinoic acid, methyl ester) obeyed Lipinski's criteria, had remarkable pharmacokinetic profiles, and their docking analyses revealed that they modulated antioxidant enzymes with better binding affinities than vitamin C.</p><p><strong>Conclusion: </strong>Given the demonstrated antidotal and cardioprotective properties of the plant root, it presents a promising candidate for mitigating doxorubicin-induced toxicities and could be effectively utilized as a supportive therapy in such contexts.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"200"},"PeriodicalIF":3.3,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12135251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of pregnant and breastfeeding women's intention to use traditional Chinese medicine: a cross-sectional study from China.","authors":"Zanmei Li, Jiao Li, Yushan Lan, Liangkun Ma, Lei Xuan","doi":"10.1186/s12906-025-04952-5","DOIUrl":"10.1186/s12906-025-04952-5","url":null,"abstract":"<p><strong>Background: </strong>Traditional Chinese medicine (TCM) is widely used by pregnant and breastfeeding women in China, yet predictors of its use intention remain understudied in mainland China. This study applied an extended Theory of Planned Behavior (TPB) framework to identify factors influencing TCM use intention in this population.</p><p><strong>Methods: </strong>A cross-sectional survey was conducted among 264 pregnant and breastfeeding women from diverse regions in mainland China between July and October 2023. Data were collected through an online questionnaire that included demographic information, TPB-based measures (attitudes, subjective norms, perceived behavioral control, intention), and past TCM use experiences. Structural Equation Modeling (SEM) was used to test hypothesized relationships.</p><p><strong>Results: </strong>The results showed that 37.5% of participants reported using TCM during pregnancy or lactation. The extended TPB model explained 84.5% of variance in intention, with subjective norms having the strongest impact (β = 0.435; p < 0.001), followed by attitudes (β = 0.304; p < 0.001) and perceived behavioral control(β = 0.294; p < 0.001). Past experience with TCM also significantly influenced both attitudes and intentions. The model demonstrated excellent fit (χ²/df = 1.53; CFI = 0.99; RMSEA = 0.04).</p><p><strong>Conclusions: </strong>This study demonstrates that TCM use during pregnancy and lactation is common in China, and that subjective norms, attitudes, perceived behavioral control, and prior experience are key predictors of intention to use TCM. These findings underscore the importance of integrating cultural and social considerations into healthcare practices. Healthcare providers should be aware of the significant role of family in decision-making and incorporate culturally sensitive counseling into maternal care. Policymakers should strengthen regulatory oversight of TCM to ensure its safe and effective use during pregnancy.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"199"},"PeriodicalIF":3.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first record of biological activities and chemical constituents of different extracts and semi-purified fractions of Bidens aurea (Aiton) Sherff (Asteraceae).","authors":"Shaimaa S Shoman, Randa S Hasan, Rim Hamdy, Emad A Shalaby","doi":"10.1186/s12906-025-04924-9","DOIUrl":"10.1186/s12906-025-04924-9","url":null,"abstract":"<p><p>Bidens aurea is a flowering plant known for its yellow or white flowers rich with various bioactive chemical compounds that possess or have proven high medicinal values, This research aimed to investigate the in vitro antioxidant and antimicrobial abilities of individual polar extracts and semi-purified fractions of Bidens aurea. In this study, three different extracts (cold water, hot water, and ethanol) and 6 isolated fractions of ethanolic extract (as the most potent sample) were assessed for their antiradical and antioxidant properties in comparison with ascorbic acid a natural standard with 2,2-diphenyl- 1-picrylhydrazyl (DPPH^•) and 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) radical scavenging assays, in addition to, GC-MS/MS identification of fractions and biochemical compounds. The findings revealed that, both methods were correlated in which the ethanolic extract showed the highest antioxidant effect with 61.71 ± 0.22 and 87.29 ± 0.56% in comparison with ascorbic acid as the natural standard (95.12 ± 0.29 and 99.32 ± 0.08%) at 1000 µg/ml against DPPH and ABTS, respectively. The fraction no. 6 (ethanol 100%) recorded the highest antioxidant activity by 90.40 ± 0.13 and 94.34 ± 0.18% at 1000 µg/ml respectively followed by fraction no. 5 (ethyl acetate: ethanol 20:80) by 82.61 ± 0.19 and 87.00 ± 0.48% at 1000 µg/ml, respectively. The antimicrobial effect revealed that the ethanolic extract of Bidens aurea was effective against all tested microorganisms with growth inhibition zones (IZ) diameters ranging from 21 to 33 mm (mm). Also, the crude ethanolic extract recorded the highest antimicrobial potential against all tested microorganisms except Bacillus cereus when compared with the extract fractions and antimicrobial standards (Fluconazole and gentamycin). The GC-MS/MS analysed of different Bidens fractions revealed the presence of various phyto-components. Totally 26 constituents were identified in Bidens aurea from all the four analysed fractions. Ethyl acetate: ethanol (60:40) fraction has a recorded the highest number of (10) phyto-constituents, while Ethyl acetate: ethanol (20:80) fraction has lower number of (3) phytoconstituents. Based on these findings, the ethanolic extract and its fractions of Biden aurea exhibit promising antioxidant, antiradical, and antimicrobial activities, suggesting their potential for further investigation in biomedical application.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"198"},"PeriodicalIF":3.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of herbal products for gas pain in children: a questionnaire-based study and alkaloid content analysis.","authors":"Semih Bulut, Nazmi Mutlu Karakaş, Ayhan İbrahim Aysal, Didem Deliorman Orhan","doi":"10.1186/s12906-025-04938-3","DOIUrl":"10.1186/s12906-025-04938-3","url":null,"abstract":"<p><strong>Background: </strong>Herbal products have been used for gas pains in children for many years. However, the quality of herbal products used in children and the presence of contamination in the products are controversial. This study was conducted to evaluate the frequency of use of herbal products for gas pain in pediatric patients, the attitudes of parents towards the use of herbal products, and the pyrrolizidine alkaloid content of herbal products used for gas pain.</p><p><strong>Methods: </strong>The survey part of the study was conducted between 15.06.2020-15.09.2020 at Gazi University Hospital Pediatrics Clinics. The surveys were conducted face to face with the parents. The Statistical Package for Social Sciences (SPSS) 23 program was used in the analysis of the data. In the other part of the study, 28 herbal products frequently used in gas pain were purchased from spice shops, markets and internet sites and their pyrrolizidine alkaloid content was evaluated by LC-QTOF-MS analysis.</p><p><strong>Results: </strong>31.5% of the participants had their children use herbal products for gas pains. When the plants used for gas pains in children were examined, fennel came first with a usage rate of 51.3%. The plants used in gas pains were purchased from spice shops by most of the participants (59%). The presence of pyrrolizidine alkaloids above 10 µg/kg concentration was detected in 75% of herbal products used in gas pains.</p><p><strong>Conclusion: </strong>In all segments of society, children are given herbal products for gas pains. Parents mostly buy herbal products from spice shops. Products sold for gas pains may cause hepatotoxic effects in children when consumed for a long time and in high doses due to the pyrrolizidine alkaloids they contain. Herbal products to be used in gas pain should be used under the consultancy of a physician/pharmacist and should be obtained from pharmacies.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"195"},"PeriodicalIF":3.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalus polysaccharide protects against cardiac injury in a tnnt2a mutant zebrafish model of dilated cardiomyopathy.","authors":"Chang Zhou, Hui Zhao, Longping Peng, Yidan Dong, Qiong Wu, Xu Wang, Yingjia Xu, Youhua Wang","doi":"10.1186/s12906-025-04925-8","DOIUrl":"10.1186/s12906-025-04925-8","url":null,"abstract":"<p><strong>Background: </strong>Dilated cardiomyopathy (DCM) is a severe and irreversible heart disease characterized by dilated ventricles and decreased myocardial function. DCM has a poor prognosis and a very low survival rate, with a 5-year mortality rate ranging from 15 to 50%, and is an important cause of sudden cardiac death and heart failure. Genetic factors play important roles in the pathogenesis of DCM. Mutations in the cardiac troponin T (tnnt2) gene represent an important subset of known pathogenic variants that bind to DCM. However, few specific drugs are currently available to treat DCM caused by these gene mutations. Astragalus polysaccharide (APS), the main active ingredient of Astragalus mongholicus Bunge (Huangqi), is widely used in China to treat cardiovascular diseases, including DCM. This study explored drugs for the treatment of DCM caused by tnnt2a mutation and revealed the protective effect of APS on tnnt2a-mutant dilated cardiomyopathy.</p><p><strong>Methods: </strong>The tnnt2a^-/- mutant zebrafish were used as a DCM model for comparison with the APS-treated group. The survival rate and the sinus venosus‒bulbus arteriosus (SV‒BA) distance were used to observe changes in cardiac output. Histopathological changes were observed via hematoxylin and eosin (HE) staining and TUNEL staining. The transcriptomes of the zebrafish in the DCM group and APS-treated group were investigated via RNA-seq. qRT‒PCR detection of apoptosis-related gene expression.</p><p><strong>Results: </strong>We found that APS markedly increased the heart rate and ATP content, and significantly inhibited the level of cardiac tissue edema, which are essential for improving the survival rate of tnnt2a^-/-. Furthermore, APS modulates key muscle fiber-related genes (including ttnb and myom3) and significantly impacts multiple signaling pathways, including Rap1, PI3K-Akt, Jak-STAT, and Wnt signaling. The qRT‒PCR results revealed that APS decreased the expression of bax, caspase-3, and caspase-9 but increased the expression of bcl-2 in DCM zebrafish.</p><p><strong>Conclusions: </strong>Our findings suggest that APS can improve the survival rate in dilated cardiomyopathy and has a positive protective effect on the myocardium in the tnnt2a mutant zebrafish model of DCM.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"197"},"PeriodicalIF":3.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol reverses cassava (Manihot esculenta Crantz) juice-induced motor impairment in the Wistar rat.","authors":"Eduardo Rivadeneyra-Domínguez, Isaac Zamora-Bello, Erik Raúl Juárez-Zaragoza, Óscar Rosales-Sánchez, Gabriel Guillén-Ruiz, Juan Francisco Rodríguez-Landa","doi":"10.1186/s12906-025-04934-7","DOIUrl":"10.1186/s12906-025-04934-7","url":null,"abstract":"<p><strong>Background: </strong>Long-term cassava (Manihot esculenta Crantz) intake has been associated with the development of neurological diseases characterized by motor impairment in humans and experimental animals. Thus, there is a need to identify the therapeutic effects of molecules to ameliorate said alterations, such as resveratrol, which was explored in the present study. Therefore, we evaluate whether the behavioral alterations associated with the chronic intake of cassava juice could be reversed with resveratrol.</p><p><strong>Methods: </strong>Adult male rats were randomly assigned to four independent groups (n = 8): vehicle (purified water), cassava (28.56 mg/kg), resveratrol (10.70 mg/kg), and a combination of treatments (cassava plus resveratrol). Vehicle and cassava juices were administered from days 1 to 28, followed by vehicle or resveratrol from days 29 to 56. The effects of the treatments were evaluated on days 28 and 56 in the open field test, rotarod, and swimming test, compared with the baseline.</p><p><strong>Results: </strong>Cassava juice increased crossing, rearing, and grooming in the open field, produced a short latency to fall from the rotarod, and increased the spin behavior and the total time of immobility in the swimming test. These effects were reversed by resveratrol.</p><p><strong>Conclusions: </strong>Resveratrol could be considered in the development of therapeutic strategies for neurological disorders associated with cassava consumption.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"193"},"PeriodicalIF":3.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing skin permeation of Phlai oil and ginger extracts through lipid nanoparticle encapsulation for anti-inflammatory topical products.","authors":"Somkamol Intawong, Kanyanat Kaewiad, Thanchanok Muangman, Worawut Kriangkrai","doi":"10.1186/s12906-025-04932-9","DOIUrl":"10.1186/s12906-025-04932-9","url":null,"abstract":"<p><strong>Background: </strong>The increasing demand for effective and safe pain management solutions, particularly for chronic conditions, has led to a focus on topical products that provide targeted relief with minimal systemic side effects. This study aims to develop an innovative anti-inflammatory topical formulation utilizing phlai (Zingiber cassumunar) oil and ginger (Zingiber officinale) extract, both of which are recognized for their potent anti-inflammatory properties.</p><p><strong>Methods: </strong>Nanostructured lipid particles (NPOs) were prepared using a hot homogenization technique followed by ultrasonication, incorporating varying ratios of ginger extract and phlai oil. The physicochemical properties, encapsulation efficiency, and stability of the NPOs were systematically investigated. In vitro permeation studies were conducted using Franz diffusion cells to assess skin penetration. The anti-inflammatory efficacy was evaluated in RAW 264.7 macrophage cells by measuring levels of nitric oxide (NO), TNF-α, and PGE2.</p><p><strong>Results: </strong>The NPO formulation improved skin penetration of 6-gingerol compared to conventional solutions, as evidenced by higher cumulative permeation and flux values. Fluorescence microscopy confirmed deeper skin penetration of Nile red dye when delivered via the NPOs. The combination of phlai oil and ginger extract showed enhanced anti-inflammatory activity, inhibiting NO production by up to 75.98% and TNF-α by 70.03%. Additionally, the combination treatment inhibited PGE2 levels by 62.34%, which was greater than the effects observed with each individual extract. These results indicate that the NPO-based formulation not only enhances the delivery of bioactive compounds but also enhances their therapeutic potential through combined action.</p><p><strong>Conclusions: </strong>The findings suggest that NPO-based formulations have the potential to effectively deliver herbal extracts transdermally, improving skin penetration and anti-inflammatory effects. This approach offers a promising avenue for developing natural topical pain relief solutions, leveraging the therapeutic benefits of traditional herbal medicine. By enhancing dermal absorption and ensuring stability, this study contributes to the development of safe and effective topical products for integrative health care. However, further research, including clinical trials and in vivo studies, is needed to validate the efficacy and safety of this formulation compared to existing treatments.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"196"},"PeriodicalIF":3.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}