BMC Complementary Medicine and Therapies最新文献

{"title":"Correction: Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells.","authors":"Seyedeh Mona Mousavi Esfahani, Parastoo Tarighi, Kosar Dianat, Tabarek Mahdi Ashour, Negar Mottaghi-Dastjerdi, Mehdi Aghsami, Mahsa Sabernavaei, Hamed Montazeri","doi":"10.1186/s12906-024-04714-9","DOIUrl":"10.1186/s12906-024-04714-9","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"408"},"PeriodicalIF":3.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between acupuncture dose and pregnancy outcomes in women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer: a systematic review.","authors":"Yi Ting Li, Chao Liang Li, Han Yang, Lei Huang, Jia Jia Liu, Xiao Yan Zheng, Xin Yue Tao, Zheng Yu, Fan Rong Liang, Xiao Ping Tian, Jie Yang","doi":"10.1186/s12906-024-04695-9","DOIUrl":"10.1186/s12906-024-04695-9","url":null,"abstract":"<p><strong>Background: </strong>Increasing studies focused on the efficacy of acupuncture on pregnancy outcomes in patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization-embryo transfer (IVF-ET). However, debatable conclusions have been drawn from different randomized controlled trials (RCTs), which might be related to different doses of acupuncture.</p><p><strong>Objective: </strong>To evaluate whether acupuncture has a dose-dependent effect on pregnancy outcomes in patients with PCOS undergoing IVF-ET in systematically reviewing.</p><p><strong>Methods: </strong>Seven electronic databases were searched from inception to October 10th, 2024. The Cochrane Collaboration's tool ROB 2.0 (ROB 2.0) provided an assessment for the risk of bias. The acupuncture dose was extracted, then categorized into high, medium, and low dose according to the scoring system results, the evidence was assessed by Slavin's qualitative best-evidence synthesis approach in a rigours methodological way. Clinical pregnancy rate (CPR) was regarded as the primary outcome.</p><p><strong>Results: </strong>A total of 953 subjects met the eligibility criteria in 12 RCTs were included, among which two studies were low dose, four were medium dose, and six were high dose. The overall quality of included studies was low, 50.00% (6/12) studies were low risk, 16.67% (2/12) studies were some concerns, and 33.33% (4/12) studies were high risk. Comparing the results, the consistent high-dose result among high-quality trials provides strong evidence for a positive correlation between high-dose acupuncture and pregnancy outcomes.</p><p><strong>Conclusion: </strong>A trend indicates that higher acupuncture doses yield better outcomes for PCOS patients undergoing IVF-ET. Further confirmation through direct comparisons of different doses was needed.</p><p><strong>Trial registration: </strong>The systematic review has been registered on PROSPERO ( https://www.crd.york.ac.uk/prospero/ ), and the registration number is CRD42023400187.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"407"},"PeriodicalIF":3.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Mentha on blood pressure: a GRADE-assessed systematic review and meta-analysis of randomized controlled trials.","authors":"Faezeh Nematolahi, Abbas Mohtashamian, Ghazal Kaveh, Nasrin Sharifi, Alireza Milajerdi","doi":"10.1186/s12906-024-04701-0","DOIUrl":"10.1186/s12906-024-04701-0","url":null,"abstract":"<p><strong>Background: </strong>Mentha consumption may associated with blood pressure improvement in humans, but the recent evidence from randomized controlled trials (RCTs) showed inconsistent results. The present study provides a systematic review and meta-analysis of RCTs to investigate the effect of Mentha on blood pressure.</p><p><strong>Methods: </strong>To cover all relevant literature, a complete search was conducted across PubMed, ISI Web of Science, and SCOPUS databases before March 2024 using PRISMA guidelines. In addition, Google Scholar, SID databases, the reference lists of the related reviews, and meta-analyses were searched for this purpose. Also, a \"snowball search\" was applied to include other relevant trials that may have been missed. A random-effects model was used for quantitative data synthesis, with weight mean difference (WMD) and 95% confidence intervals (CI). Standard methodologies were utilized to assess kappa statistics between the authors, GRADE evidence profiles, heterogeneity, meta-regression, sensitivity analysis, and publication bias.</p><p><strong>Results: </strong>Out of 476 publications identified, seven RCTs were eligible and included in this systematic review and meta-analysis. There was perfect agreement in study selection between the reviewers (К statistic, 0.86; p < 0.001). Meta-analysis showed a 1.227 mmHg reduction in systolic blood pressure (SBP) (95% CI: -6.61,4.16, p = 0.655), 2.997 mmHg reduction in long-term SBP (95% CI: -8.00,2.00, p = 0.241), 1.830 mmHg reduction in diastolic blood pressure (DBP) (95% CI: -5.06,1.40, p = 0.268), and 2.857 mmHg reduction in long-term DBP (95% CI: -6.01, 0.30, p = 0.076) after Mentha consumption in intervention group compared to control. In sub-group analysis, a statistically and clinically significant reduction in SBP and DBP was observed in the participants with ages above 30 years and in the participants with SBP > 130 mmHg or DBP > 80 mmHg.</p><p><strong>Conclusions: </strong>Our findings showed that Mentha consumption might not have a statistically significant effect on lowering SBP, DBP, long-term SBP, and long-term DBP. However, it can lead to a clinically significant reduction in both long-term SBP and long-term DBP. Besides, Mentha may have potential benefits for patients with pre-hypertension and hypertension. Nevertheless, further well-designed RCTs are needed to confirm our results. PROSPERO Registration No: CRD42023459490.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"406"},"PeriodicalIF":3.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of 3,3'-diindolylmethane on the urinary estrogen profile of premenopausal women.","authors":"Mark Newman, Jaclyn Smeaton","doi":"10.1186/s12906-024-04708-7","DOIUrl":"10.1186/s12906-024-04708-7","url":null,"abstract":"<p><strong>Background: </strong>3,3'-diindolylmethane (DIM) is a phytonutrient derived from cruciferous vegetables that is an often-used supplement in the complementary and alternative medicine space. The most common goal for providers when recommending DIM to their patients is to alter estrogen metabolism, yet research into DIM's effect on the estrogen profile is lacking in the published literature. The objective of this study was to comprehensively evaluate DIM's effect on the urinary estrogen profile.</p><p><strong>Methods: </strong>In this retrospective cohort study, we analyzed data from a clinical laboratory, including urinary estrogen and estrogen metabolite concentrations. Analyte concentrations were determined from dried urine samples using a gas chromatography-tandem mass spectrometry assay. Individuals were separated into two groups, either reporting taking DIM (N = 909) or reporting not taking DIM (N = 18,385). Comparisons between individuals in these two groups were made using the Wilcoxon rank sum test. Additionally, we were also able to explore a subset of women who had laboratory results in the database before and after initiating DIM treatment (N = 53). In this subset, differences were assessed with Wilcoxon signed rank tests.</p><p><strong>Results: </strong>In the larger group that was separated into women reporting either DIM use or no use, significant differences were observed in the concentrations of almost every urinary estrogen and estrogen metabolite (with the only exception being 2-methoxyestrone) in the urinary estrogen profiles of those taking DIM compared to those not taking DIM (all P values < 0.001). In the smaller subset of individuals with results before and after initiating DIM use, differences were only seen in 4 of the urinary estrogens and estrogen metabolites (P < 0.001 for estrone, estradiol, estriol, and 16-hydroxyestrone). Differences in total estrogens were significant in both the larger group and the smaller subset (both with P < 0.001). Additionally, observed differences in the ratios of metabolites followed a similar trend with more significant differences observed in the larger group. Notably, the 2-hydroxyestrone:16-hydroxyestrone ratio increased significantly in both the larger group and the smaller subset with results before and after DIM use.</p><p><strong>Conclusions: </strong>The results of this study provide the most comprehensive evaluation to date of DIM's effect on the urinary estrogen profile. Additionally, the results demonstrate that the dried urine collection and accompanying assay used capture changes that are similar in direction, but not necessarily magnitude, to previous reports in the literature. Considered together, these two things highlight the clinical validity and utility of this approach to the evaluation of DIM supplementation and suggest the need for additional studies using this approach to fully understand the potential clinical utility of DIM.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"405"},"PeriodicalIF":3.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study protocol for a randomized controlled trial evaluating the effectiveness of a mother-child intervention model of neurogenic tremor as an add-on to treatment for emotional disorders in adolescents.","authors":"Yanli Zhao, Jiahua Xu, Jie Zhang, Jiaqi Song, Ning Yang, Hongzhen Fan, Zhe Dong, Shuping Tan","doi":"10.1186/s12906-024-04650-8","DOIUrl":"10.1186/s12906-024-04650-8","url":null,"abstract":"<p><strong>Background: </strong>Adolescents exhibit a high prevalence of mental health disorders, with more than half of all cases emerging before the age of 14 years. Since the advent of the COVID-19 pandemic, there has been a marked upsurge in anxiety and depression among adolescents across several nations. Emotional disorders often lead to severe outcomes, including school absenteeism, self-harm, and suicidal tendencies. The suboptimal efficacy of pharmacotherapy, compounded by limited availability and substantial costs associated with individual psychotherapy, underscores the critical need for identifying simple yet efficacious psychotherapeutic interventions suitable for both individual and group settings. Tension and Trauma Release Exercise (TRE) is a mind-body therapeutic approach that efficiently alleviates symptoms of anxiety and depression. This randomized controlled trial (RCT) aims to evaluate the effectiveness of a mother-child intervention model using TRE in enhancing the clinical management of adolescent patients diagnosed with emotional disorders.</p><p><strong>Methods: </strong>This study recruits 140 dyads of adolescents with emotional disorders and their mothers, randomly assigned to intervention or control groups. The intervention arm combines eight weeks of standard pharmacotherapy with an eight-week TRE group therapy, assessing at baseline, post-8-week treatment, and three-month follow-up. Initially, controls receive eight weeks of standard medication with parallel assessments, later transitioning to the same TRE intervention while maintaining continuous evaluation. The study further examines the influence of maternal emotional health on adolescent treatment response and investigates associated neurophysiological and psychological mechanisms.</p><p><strong>Discussion: </strong>This research endeavors to identify a straightforward and potent body-oriented psychological intervention that could improve the clinical outcomes for adolescent patients with emotional disorders. Such findings would carry profound implications not only for the healthy development of teenagers but also for potentially mitigating the burden on families, educational institutions, and society as a whole.</p><p><strong>Trial registration: </strong>ChiCTR2100044553, Registered March 24, 2021.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"403"},"PeriodicalIF":3.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceptions of HIV and mental illness as \"Western\" or \"Traditional\" illnesses: a cross-sectional study from Limpopo Province, South Africa.","authors":"Michael Galvin, Lezanie Coetzee, Patricia Leshabana, Nthabiseng Masebe, Shitshembiso Lebepe, Aneesa Moolla, Amanda R Tarullo, Peter C Rockers, Denise Evans","doi":"10.1186/s12906-024-04700-1","DOIUrl":"10.1186/s12906-024-04700-1","url":null,"abstract":"<p><p>Although Western biomedical treatment has dramatically increased across sub-Saharan African health systems, traditional medicine as a form of healing and beliefs in supernatural powers as explanations for disease remain prevalent. Research in this region has identified HIV in particular as a disease located within both the traditional African and Western medical paradigms, whilst mental illness is ascribed to primarily supernatural causes. Within this context, this study sought to understand and explore the perceptions of HIV and mental illness among a population of rural women in Limpopo, South Africa. 82 in-depth interviews were conducted between January and December, 2022. Interviews were transcribed and translated into English. Data were managed using NVivo 11 software and thematically analyzed. The majority of participants identified HIV as a Western illness requiring biomedical treatment with an origin largely attributed to biological mechanisms. A traditional form of HIV only cured using traditional treatments was also denoted. Unlike for HIV, the majority of respondents felt that there was no biological or behavioral cause for mental illness but rather the illness was conceptualized supernaturally thus likely impacting patient care pathways. Further research to study HIV and mental health perceptions among a larger sample in different regions of sub-Saharan Africa is warranted.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"404"},"PeriodicalIF":3.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a poly-herbal plant extract on the adhesion of Streptococcus mutans to tooth enamel.","authors":"Cynthia J Henley-Smith, Anna-Mari Kok, Francien S Botha, Chantelle Baker, Namrita Lall","doi":"10.1186/s12906-024-04707-8","DOIUrl":"10.1186/s12906-024-04707-8","url":null,"abstract":"<p><strong>Background: </strong>Dental caries, also known as tooth decay or cavity formation, is one of the world's most widespread dental conditions. It is a plaque-related infection caused mainly by Streptococcus mutans. People have relied on several plant species to treat oral infections; Heteropyxis natalensis, for example, has been used to treat toothache and gum infections.</p><p><strong>Methods: </strong>In this study, the antimicrobial and anti-adherence properties of H. natalensis and Camellia sinensis, as well as tea tree and peppermint essential oils were investigated on tooth enamel.</p><p><strong>Results: </strong>The bacterial load of S. mutans was reduced by approximately two orders of a magnitude after 48 h, with a lesser extent on the commensal bacteria, Lactobacillus paracasei. Scanning electron micrographs of enamel blocks showed a reduction in the attachment and chain formation of S. mutans and degraded cell morphology. Lastly, the combination and each component individually, showed low to no cellular toxicity when tested on human macrophages.</p><p><strong>Conclusions: </strong>This is the first report of this polyherbal regarding its selectivity and potential prevention of dental caries.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"402"},"PeriodicalIF":3.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11575210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gardenia jasminoides fruit extract alleviates non-steroidal anti-inflammatory drug-induced gastropathy in rats.","authors":"Rinrada Worapongpaiboon, Kasiphak Kaikaew, Pornpen Werawatganone, Kanjana Somanawat, Nathawadee Lerttanatum, Naruemon Klaikeaw, Duangporn Werawatganon","doi":"10.1186/s12906-024-04702-z","DOIUrl":"10.1186/s12906-024-04702-z","url":null,"abstract":"<p><strong>Background: </strong>NSAID-induced gastropathy is a health burden that requires effective intervention. Among various prevention options, Gardenia jasminoides fruit extract (GJE) has demonstrated gastroprotective effects through anti-inflammatory pathways with a wide safety margin. However, the detailed molecular mechanisms of GJE regarding mucoprotective and anti-inflammatory effects remained to be explored. Therefore, we investigated the effects of GJE on NSAID-induced gastric injury in rats, focusing on the expression of the protective factors: prostaglandin E₂ (PGE₂) and mucin 5AC (MUC5AC), and the aggravating factors: inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB).</p><p><strong>Methods: </strong>Twenty-four male Sprague-Dawley rats were assigned to three experimental groups (n = 8/group): the control group, the NSAIDs group receiving indomethacin to induce gastric ulcers, and the NSAIDs with GJE pretreatment (NSAIDs + GJE) group. After a two-day experimental period, the stomachs were collected for histopathological examination, immunohistochemical staining, and protein expression analysis in gastric tissue lysates.</p><p><strong>Results: </strong>The NSAIDs group exhibited severe neutrophil infiltration with ulcers upon gastric histopathological examination. Pretreatment with GJE attenuated NSAID-induced gastropathy, as evidenced by reduced neutrophil infiltration and decreased ulceration. Immunohistochemical staining and Western blotting demonstrated reduced expressions of PGE₂ and MUC5AC, while the expressions of iNOS and NF-κB were increased following NSAID administration. In comparison to the NSAIDs group, the NSAIDs + GJE group exhibited higher expressions of PGE₂ and MUC5AC and lower expressions of iNOS and NF-κB, providing evidence of the gastroprotective effects of GJE.</p><p><strong>Conclusions: </strong>Pretreatment with GJE alleviated NSAID-induced gastric ulcers by increasing the expression of PGE₂ and MUC5AC and decreasing the expression of iNOS and NF-κB. This study contributes to the understanding of the mechanisms by which GJE attenuates NSAID-induced gastropathy. Further studies are required to validate the effect of GJE in clinical settings.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"401"},"PeriodicalIF":3.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tongguanteng injection exerts anti-osteosarcoma effects through the ER stress-associated IRE1/CHOP pathway.","authors":"Xiao-Chuan Xue, Yang-Yun Zhou, Ling-Yan Xu, Lan-Yi Wei, Yu-Jie Hu, Jiao Yang, Xiang-Qi Zhang, Meng-Yue Wang, Yong-Long Han, Jun-Jun Chen","doi":"10.1186/s12906-024-04689-7","DOIUrl":"10.1186/s12906-024-04689-7","url":null,"abstract":"<p><strong>Background: </strong>In China, Tongguanteng injection (TGT) is widely used in the treatment or adjuvant treatment of various types of cancer. However, the effect and mechanism of TGT in osteosarcoma is not clear.</p><p><strong>Methods: </strong>The 143B and MG-63 cells were treated with different concentrations of TGT. Cell proliferation, migration, invasion and apoptosis were detected using CCK8 assay, transwell assay and flow cytometry. Differentially expressed genes (DEGs) were screened using RNA sequencing (RNA-seq). The identified mRNA and protein expression associated with the IRE1/CHOP pathway was validated by RT-PCR and western blot assay. To explore the underlying mechanisms, 4-phenylbutyric acid (4-PBA) was selected as a specific endoplasmic reticulum (ER) stress inhibitor. Small interfering RNA (siRNA) or pEX-3-ERN1 plasmid was transfected into 143B cells to silence or overexpress IRE1, respectively. The potential downstream proteins, including CHOP, and apoptosis associated proteins, caspase-3 and PARP1 were determined. Furthermore, the effect of TGT was demonstrated in 143B cell tumor-bearing mice in vivo. H&E staining, TUNEL staining and immunohistochemistry were conducted in tumor tissues obtained from the xenograft mouse model.</p><p><strong>Results: </strong>TGT was shown to dramatically suppress the proliferation, migration and invasion, and induce apoptosis of osteosarcoma 143B and MG-63 cells in vitro. The identified DEGs included HSPA5 (encoding BiP) and ERN1 (encoding the IRE1 protein), as well as apoptosis-associated gene DDIT3 (encoding the CHOP protein). The term \"IRE1-mediated unfolded protein response\" was screened to be the most enriched biological process GO term. The expression of ER stress-associated proteins including ATF6, BiP, p-IRE1, XBP1s and CHOP, as well as apoptosis-associated cleaved caspase-3 and cleaved PARP1 proteins, was significantly upregulated by TGT treatment in osteosarcoma 143B cells, suggesting that TGT might promote the apoptosis of osteosarcoma 143B cells through the IRE1/CHOP pathway. Furthermore, knocking down IRE1 with si-IRE1 or inhibiting of ER stress with 4-PBA suppressed the expression of ATF6, BiP, XBP1s and CHOP induced by TGT, as well as the expression of cleaved caspase-3 and cleaved PARP1. On the contrary, overexpressing IRE1 promoted CHOP expression and induced osteosarcoma cell apoptosis. Consistent with in vitro results, TGT dramatically inhibited the tumor growth and promoted the expression of p-IRE1 and CHOP in tumor-bearing mice.</p><p><strong>Conclusion: </strong>The findings suggest that TGT exerts an anti-osteosarcoma effect in vitro and in vivo. The underlying mechanism might be associated with the activation of IRE1/CHOP pathway in ER stress. Our findings suggest that targeting IRE1/CHOP pathway might be a potential novel approach for osteosarcoma treatment.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"400"},"PeriodicalIF":3.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive effect of clove essential oil combined with hydrogen inhalation improves psychological harm caused by lipopolysaccharide in mice.","authors":"Wei-Wen Sung, Tsung-Ming Yeh, Wen-Ling Shih","doi":"10.1186/s12906-024-04682-0","DOIUrl":"10.1186/s12906-024-04682-0","url":null,"abstract":"<p><strong>Background: </strong>Psychological anxiety and depression, as well as memory impairment, are frequently linked to inflammation. Clove essential oil (CEO) administration and hydrogen (H₂) inhalation have been proven to have anti-inflammatory and alleviating effects on related psychological disorders in the past. The current study investigated the potential to improve anxiety-like behaviors and cognitive function by a combination of CEO and H₂ treatment.</p><p><strong>Methods: </strong>The mice were administered lipopolysaccharide (LPS) to induce inflammation and oxidative stress response and cause psychological disorders. Using this animal model, we conducted experiments to test whether essential oil and H₂ inhalation could improve the psychological damage in behavior caused by LPS. Subsequently, elevated plus maze (EPM), forced swimming test (FST), and passive avoidance (PA) test were performed for evaluation of mice anxiety, depression, and response to electric shock, respectively. Furthermore, the biochemical analysis was used to examine the expression levels of inflammatory and oxidative stress markers.</p><p><strong>Results: </strong>Our results showed that CEO administration and H₂ inhalation alone or in combination positively improved inflammation-induced anxiety, depression, and cognitive memory deficits in the mice. In the single treatment groups, CEO demonstrated better results than H₂ inhalation in the elevated plus maze, forced swimming, and passive avoidance tests, while combined treatment with both provided a further improved enhancement effect. Biochemical analysis of the cerebral cortex revealed that CEO and H₂ therapy reversed the LPS-induced inflammation and oxidative stress response.</p><p><strong>Conclusions: </strong>Our results suggest that a combination of CEO and H₂ has the potential to treat psychological disorders or neuropsychiatric disorders.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"399"},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}