BMC Complementary Medicine and Therapies最新文献

{"title":"Efficacy and safety of curcumin in patients with hand osteoarthritis: a pilot double-blinded randomised controlled trial.","authors":"Panithan Tuntiyatorn, Chawanin Lerspongpaibool, Thepparat Kanchanathepsak, Ittirat Watcharananan, Tulyapruek Tawonsawatruk","doi":"10.1186/s12906-025-05096-2","DOIUrl":"10.1186/s12906-025-05096-2","url":null,"abstract":"<p><strong>Background: </strong>Curcumin, a substance in Turmeric spices, has been shown to exhibit anti-inflammatory and chondroprotective properties in several preclinical studies. However, there is limited research on the efficacy and side effects of oral curcumin in treating hand osteoarthritis. Thus, the primary aim of this pilot study was to assess the therapeutic effects and potential adverse effects of curcumin in patients with hand osteoarthritis, specifically in terms of pain reduction, improvement in functional outcomes, and relevant blood test parameters.</p><p><strong>Method: </strong>This pilot prospective double-blind, randomized controlled trial included patients over 50 years old diagnosed with hand osteoarthritis. Participants received a three-month oral administration of either a turmeric capsule containing curcumin (170 mg/day) or a placebo. The outcomes measures included the Visual Analogue Scale (VAS) for pain, the Disabilities of Arm, Shoulder, and Hand (DASH) score, hand grip and pinch strength, as well as side effects and blood tests at one, two, three, and six months.</p><p><strong>Results: </strong>Twenty-seven patients were enrolled in this study-13 in the curcumin group and 14 in the placebo group. Baseline characteristics were similar between both groups. Although patients in the curcumin group showed reductions in VAS pain scores and improvements in DASH scores after one month, the differences were not statistically significant. However, by the third month, patients in the curcumin group demonstrated significant reductions in VAS pain scores at rest and during activity compared to the baseline scores, with mean differences of 1.37 ± 0.64 (p-value = 0.032) and 1.74 ± 0.73 (p-value = 0.017), respectively. Additionally, their DASH scores improved significantly with a mean difference of 11.84 ± 5.88 (p = 0.044). One patient in the curcumin group experienced gastrointestinal (GI) side effects, leading to withdrawal from the study, but no other adverse effects were reported.</p><p><strong>Conclusion: </strong>Based on the results of this pilot study, the authors conclude that a three-month regimen of low-dose oral curcumin may improve pain and functional scores in patients with hand osteoarthritis compared to placebo. However, GI discomfort remains a potential side effect to monitor. Overall, curcumin appears to be a safe and feasible alternative treatment option for hand osteoarthritis, and a larger, definitive randomised controlled trial is warranted.</p><p><strong>Trial registration: </strong>The trial has been registered in the Thai Clinical Trials Registry(TCTR) database. The identification number is TCTR20210929003.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"356"},"PeriodicalIF":3.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12502247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of bioactive and anti-inflammatory molecules of Thymbra spicata L. from Mardin by GC-MS and LC-Orbitrap HRMS: a DFT, molecular docking, ADMET, biological target and activity study.","authors":"Velid Unsal, Leyla Ercan, Cemile Gunbegi Calıskan","doi":"10.1186/s12906-025-05054-y","DOIUrl":"10.1186/s12906-025-05054-y","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"358"},"PeriodicalIF":3.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12502334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vapor phase of white thyme essential oil: effect on Candida albicans and preservation of Lactobacillus species in the context of vulvovaginal candidiasis.","authors":"Liliana Fernandes, Inês Silva, Daniela Araújo, Raquel Costa, Sónia Silva, Nuno Pereira Mira, Sofia Costa-de-Oliveira, Mariana Henriques, Maria Elisa Rodrigues","doi":"10.1186/s12906-025-05067-7","DOIUrl":"10.1186/s12906-025-05067-7","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"357"},"PeriodicalIF":3.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12502485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-marketing safety evaluation of Guhong Injection: a multicenter, prospective real-world study of 30,000 cases in China.","authors":"Jiayu Deng, Tianzi Lei, Xiangfeng Wang, Changxin Liu, Yanqing Song","doi":"10.1186/s12906-025-05112-5","DOIUrl":"10.1186/s12906-025-05112-5","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the incidence, nature, and clinical manifestations of adverse events (AEs) and adverse drug reactions (ADRs) associated with Guhong Injection, and to provide evidence for the safe clinical use of this drug.</p><p><strong>Methods: </strong>A prospective, multicenter, large-scale, registry-based hospital centralized monitoring system was employed to track eligible patients using Guhong Injection across 31 hospitals in China from October 2019 to December 2022.</p><p><strong>Results: </strong>A total of 30,000 cases were included in the study, with 81 AEs reported, involving 67 patients, yielding an incidence rate of 2.23‰. Of these 67 patients, 60 were first-time users of Guhong Injection, accounting for 89.55% of the AEs. The most common ADR observed was hypersensitivity, with an incidence rate of 1.13‰, primarily presenting as skin itching (0.53‰) and rash (0.43‰). Multifactorial unconditional logistic regression analysis revealed that ethnicity, frequency of drug use, contraindications, and allergic history were significantly associated with the occurrence of AEs.</p><p><strong>Conclusion: </strong>The overall AE rate of Guhong Injection was classified as uncommon according to CIOMS guidelines. Hypersensitivity reactions, particularly skin itching and rash, were the most frequently observed ADRs. Factors such as ethnicity, frequency of drug use, contraindications, and allergic history were identified as contributing to the occurrence of AEs, providing crucial insights for ensuring the safe clinical use of Guhong Injection.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"355"},"PeriodicalIF":3.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12495768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interest in complementary and alternative medicine among participants in a study on cancer prevention by green tea extract - results from an expert-based survey of MIRACLE trial participants.","authors":"Anna Melzer, Niklas Sturm, Friederike Rohlmann, Rainer Muche, Julia Stingl, Thomas J Ettrich, Thomas Seufferlein","doi":"10.1186/s12906-025-05087-3","DOIUrl":"10.1186/s12906-025-05087-3","url":null,"abstract":"<p><strong>Objective: </strong>Although chemopreventive strategies such as acetylsalicylic acid have shown potential in cancer prevention, they also entail adverse effects. This leads to growing interest in \"natural\" compounds, such as plant-based extracts, which may offer preventive benefits with fewer side effects. However, little is known aboutindividuals' personal attitudes towards conventional medications and complementary or alternative therapies when they are eligible for various cancer screening and prevention programmes. The present study aims to investigate the attitude toward medications and various therapies, as well as their prescription, among subjects participating in a clinical trial on the prevention of colorectal adenomas using green tea extract. A further goal was to assess whether the study predominantly attracted individuals with a strong preference for natural products or a more diverse set of opinions.</p><p><strong>Methods: </strong>992 participants (age 50-80 years) of the MIRACLE study (a randomised study comparing green tea extract vs. placebo for secondary prevention of colorectal adenomas, NCT01360320) in 40 study centres across Germany received a 25-item, expert-based questionnaire regarding their attitude towards different medications and therapies including complementary and alternative medicine. Basic characteristics including age, gender, ECOG status, and lifestyle factors were also recorded. An exploratory factor analysis was used to group the items into eight thematic areas, which were then analysed for potential associations with the participants' baseline characteristics.</p><p><strong>Results: </strong>Response rate to the questionnaire was 94.3% (935/992). Survey participants mainly expressed positive attitudes towards both conventional and complementary medicine. Most were open to genetic testing for personalised treatment. Comparing the categories of the exploratory factor analysis revealed that female gender and regular exercise were associated with the regular use of alternative healing methods (i.e. acupuncture or homeopathic medicine). A more favourable view of alternative approaches was more common among younger and better-educated individuals, but the same groups also rated conventional medicine less negatively.</p><p><strong>Conclusion: </strong>The findings of this study reveal a relatively high level of openness towards both conventional and alternative medicine among participants of the MIRACLE trial, as well as certain features, such as age, gender, and education that impact on the attitude towards medications and therapies among participants in a study on secondary prevention of colorectal polyps by green tea extract.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov: NCT01360320.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"350"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective role of rice bran extract and its constituents in a neuroinflammatory mouse model.","authors":"Sarah M Abou El-Nagah, Mohammad Abdel-Halim, Ola A Heikal, Reham M AbdelKader","doi":"10.1186/s12906-025-05097-1","DOIUrl":"10.1186/s12906-025-05097-1","url":null,"abstract":"<p><strong>Background: </strong>Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor known to play a critical role in regulating neuroinflammation and neurodegenerative processes, including Alzheimer's disease. Prior studies from our group demonstrated that rice bran extract (RBE) enhances cognitive function and increases PPARγ DNA-binding activity in the brain, effects that were abolished by PPARγ antagonism. These findings suggest that bioactive constituents within RBE may modulate PPARγ signaling. The current study aimed to provide additional evidence for the involvement of PPARγ activation in the neuroprotective effects of RBE and to identify key RBE-derived components that may contribute to these effects.</p><p><strong>Methods: </strong>A neuroinflammatory mouse model was treated orally for 21 consecutive days with RBE. The brain CD36 and amyloid-beta (Aβ) protein levels were measured. HPLC and GC were used to assess the levels of RBE components. To measure alterations in fatty acid content after treatment with RBE, brain levels of DHA, EPA and AA were assessed using UHPLC/MS-MS.</p><p><strong>Results: </strong>RBE treatment increased the brain levels of CD36, the direct PPARγ target, and decreased Aβ levels. A strong correlation was detected between the Aβ and CD36 protein levels. As RBE was found to be rich in linolenic acid (ALA), linoleic acid (LA) and oleic acid, their metabolites concentrations in mice brain were measured, and results indicated higher concentration of EPA and DHA after RBE treatment.</p><p><strong>Conclusions: </strong>RBE exerts neuroprotective effects potentially through activation of the PPARγ pathway, as evidenced by CD36 upregulation and Aβ reduction. The enrichment of RBE in polyunsaturated fatty acids (PUFAs), along with the observed increase in their brain-penetrant metabolites (EPA and DHA), suggests these lipids may contribute to the cognitive benefits of RBE.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"351"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of fundamental movement skills practice on executive function and social interaction ability in children with moderate autism: a randomized controlled trial.","authors":"Qiang Wang, Shuqi Jia, Feng Ding, Xi Wang, Yanran Si, Xing Wang, Jing Sun","doi":"10.1186/s12906-025-05107-2","DOIUrl":"10.1186/s12906-025-05107-2","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of practicing fundamental movement skills (FMS) on executive function and social interaction abilities in children with moderate autism.</p><p><strong>Methods: </strong>A randomized controlled experimental design was employed. Twenty-five children with moderate autism were randomly assigned to either an experimental group or a control group. The experimental group received the FMS intervention, while the control group continued with their regular daily routine without intervention. The intervention was conducted over 18 weeks, with sessions held four times per week, each lasting 45 min at a moderate intensity. Executive function and social interaction abilities were assessed in both groups before and after the intervention.</p><p><strong>Results: </strong>Social interaction abilities, as measured by the SRS-2 scores and its sub-dimensions, showed significant improvement (P < 0.05). In terms of executive function, significant improvements were observed in the Behavior Rating Inventory of Executive Function (BRIEF) scale scores, specifically in inhibition, shifting (cognitive flexibility), emotional control, and working memory (P < 0.05). However, there were no significant improvements in initiation, planning, organization, or monitoring functions (P > 0.05).</p><p><strong>Conclusion: </strong>The FMS intervention led to improvements in both social interaction abilities and executive function in children with autism spectrum disorder. However, the improvement in executive function was selective, with certain sub-dimensions showing significant gains while others did not. This study underscores the value of cross-modal interventions that bridge motor skill development and socioemotional learning, providing a foundation for future hybrid paradigms integrating FMS and dance movement therapy (DMT)principles.</p><p><strong>Trial registration: </strong>The study protocol, based on a true experimental design, was registered with ClinicalTrials.gov (registration number ChiCTR2400087989) on August 8, 2024.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"354"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the anticancer potential of nano-encapsulated graviola extract on tongue carcinoma (SCC154) cell line: targeting the PI3K/AKT/mTOR pathway (in vitro study).","authors":"Amany Hany Mohamed Kamel, Ahmed A Abd-Rabou, Ahmed Basuoni, Nermeen AbuBakr","doi":"10.1186/s12906-025-05113-4","DOIUrl":"10.1186/s12906-025-05113-4","url":null,"abstract":"<p><strong>Background: </strong>Graviola emerged as a promising anticancer agent, with nanotechnology enhancing drugs' therapeutic potential. The purpose of this work was to explore graviola extract and its nano-platform's effects on tongue carcinoma (SCC154 cells) in vitro model.</p><p><strong>Methods: </strong>Graviola leaves extract (GLE) was isolated, and its phenolic content was identified. Three nano-formulations (F1-F3) were optimized for GLE delivery, with F1 chosen for its optimal size and stability to synthesize graviola nanoparticles (GNPs). SCC154 cells were split into three groups: group Ι (untreated SCC154 cells), group ΙΙ: SCC154 cells + ethanolic GLE, and group ΙΙΙ: SCC154 cells + GLE encapsulated in a nano-void delivery system (GNPs). In vitro tests assessed cell viability via MTT assay, cell cycle, and apoptosis by flow cytometry, DNA damage using comet assay, and gene expression of the key molecular markers (PI3K, AKT, mTOR, and GSK-3β) by quantitative real-time polymerase chain reaction. Transmission electron microscopic examination of cells was also performed.</p><p><strong>Results: </strong>GLE and GNPs reduced SCC154 cells' proliferation compared to untreated cells, with GNPs showing significantly higher cytotoxicity. Both treatments also induced apoptosis, arrested the cell cycle, and caused DNA damage with a significant pronounced effect in the GNPs-treated group. Gene expression analysis revealed a substantial decline in PI3K, AKT, mTOR, and GSK-3β in both treated groups relative to the control group, with a significant downregulation in the GNPs-treated group. Ultrastructural examination revealed severe destruction in tongue carcinoma cells of both treated groups, with substantial damage in the GNPs-treated group.</p><p><strong>Conclusion: </strong>GNPs showed a better impact than GLE in tongue carcinoma therapy, causing cytotoxicity and apoptosis, potentially through the PI3K/AKT/mTOR pathway.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"352"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiform-based Yi Jin Jing exercise against osteoarthritis of the knee: study protocol for a randomised controlled trial.","authors":"Zehao Hu, Guohua Chen, Qiuxuan Huang, Zhanxin Li, Peixin Huang, Xiangmin Li, Wenyon Lv, Chuangjian Lin, Danzi Chen, Zenan Wu","doi":"10.1186/s12906-025-05105-4","DOIUrl":"10.1186/s12906-025-05105-4","url":null,"abstract":"<p><strong>Introduction: </strong>Knee osteoarthritis (KOA) is a chronic osteoarticular disease characterized by degenerative lesions of articular cartilage, with clinical manifestations of knee pain, stiffness, and limited mobility. Patients with KOA often experience lower limb muscle strength decline and weakened joint stability due to pain, further exacerbating functional disabilities. Yijinjing, a traditional Chinese fitness qigong, features stretching and gentle movements, emphasizing the coordination of limb movements and breathing. This study innovatively combined traditional Yijinjing with Baduanjin and Wuqinxi to design a modified Yijinjing training program including three postures: upright, sitting, and recumbent. The program adopts a stepped training model, adjusting training postures according to patients' knee joint mobility, and designs targeted movements for common symptoms of KOA, aiming to provide personalized rehabilitation options for KOA patients with different severities.</p><p><strong>Methods/design: </strong>In this study, a single-blind randomised controlled trial was used and a randomised list was generated using a computerised random number generator and opaque sealed envelopes for group allocation were prepared.Strict blinding was followed and outcome evaluators were blinded until the end of the experiment, when participants would not know which group they were included in.The study was conducted on patients aged between 40 and 75 years, with stable medical conditions, who had not practised Yi Jin Jing regularly in the past.These patients were randomly divided into the following 3 groups: (1) Conventional medication group (CG); (2) Traditional Yi Jin Jing exercise group (TG); (3) Modified Yi Jin Jing exercise group (MG); the control group received only conventional medication, whereas the TG and IG groups exercised for 1 h per day on top of the conventional medication for 3 months. One day per week was taken and all groups, except the control group, underwent 1 day of volitional supervised training. WOMAC was the primary outcome variables; the HSS knee score, Time Up and Go Test Active and passive knee mobility and the SF-36 scale served as secondary outcome indicators.</p><p><strong>Discussion: </strong>The present study may result in a new and widely accepted, easy-to-use, targeted and adaptable exercise prescription for Yi Jin Jing.The prescription encompasses three forms: upright, seated, and recumbent positions, along with stepped exercise patterns and targeted movements. This comprehensive approach is designed to accommodate the varying stages of KOA and the individual conditions of patients, and it is anticipated to address the shortcomings of conventional knee rehabilitation and traditional Yi Jin Jing practices.</p><p><strong>Trial registration: </strong>International Traditional Medicine Clinical Registry Platform, ITMCTR2024000234. Registered on 30 July 2024.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"353"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changji'an formula alleviates visceral hypersensitivity of a post-inflammatory IBS-D mouse model via NGF/TrkA signaling pathway.","authors":"Wei Ke, Siyu Huang, He Zhu, Qinglong Tan, Huaiguo Li, Dongwen Liu, Fanghao Zheng, Shuncong Zhang, Kaijun Lei, Hongmei Tang","doi":"10.1186/s12906-025-05095-3","DOIUrl":"10.1186/s12906-025-05095-3","url":null,"abstract":"<p><strong>Background: </strong>Changji'an Formula (CJAF) is an effective Chinese herbal prescription to treat irritable bowel syndrome with predominant diarrhea (IBS-D), which is derived from two famous classical prescription: Sijunzi decoction and Tong-Xie-Yao-Fang. However, the molecular mechanism has not been well defined. This study aims to illustrate the molecular mechanism of CJAF in the treatment of IBS-D.</p><p><strong>Methods: </strong>Chemical components of CJAF were determined by ultrahigh performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) and further verified by reference standards. IBS-D model was induced in C57BL/6J mice by a single edema with colonic infusion of 0.1 mL trinitrobenzene sulfonic acid (TNBS, 50 mg/mL) combined with 7 days of restraint stress, 2 h/d. The treatment group was given rifaximin (100 mg/kg) and high, moderate and low doses of CJAF by gavage for 7 days, respectively (n = 7). After administration, the main symptoms of IBS-D were tested, and behavioral tests were conducted using sucrose preference test and open field test. The colonic tissues of mice were obtained. Gene and protein expression of mast cell tryptase, nerve growth factor (NGF), tyrosine kinase receptor A (TrkA), phosphorylated TrkA, growth associated protein 43(GAP43) and neuro-specific enolase (NSE) were determined by RT-PCR, western blot and immunohistochemistry.</p><p><strong>Results: </strong>36 compounds were identified by UPLC-Q-Orbitrap HRMS, and the determined components can be categorized into 7 chemical types, including 16 flavonoids, 7 triterpenoids, 4 alkaloids, 3 sesquiterpenoids, 2 monoterpene glycosides, 2 organic acids, 1 phenylpropanoids and 1 tannin. Animal experiment showed that the abdominal pain and diarrhea symptoms of IBS-D mice were alleviated by CJAF. The sucrose preference, total translocation distance and average velocity of movement in the open field test was upregulated. The mRNA and protein expression of mast cell marker tryptase, as well as NGF, phosphorylated TrkA, GAP43 and NSE in IBS-D mice colonic tissues were down-regulated by CJAF.</p><p><strong>Conclusions: </strong>CJAF could effectively alleviate abdominal pain and diarrhea symptoms of IBS-D by inhibiting the activation of colonic mast cells and the resultant activation of NGF/TrkA signal pathway. Therefore, CJAF affords a potential candidate for the treatment of IBS-D.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"348"},"PeriodicalIF":3.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}