BMC Complementary Medicine and Therapies最新文献

{"title":"Based on network pharmacology and molecular docking technology to explore the pharmacodynamic components and mechanism of Gynostemmae Pentaphylli Herba reversing Cervical intraepithelial neoplasia.","authors":"Huifang Wang, Yiling Chen, Tingting Lin","doi":"10.1186/s12906-025-04841-x","DOIUrl":"10.1186/s12906-025-04841-x","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the pharmacodynamic components of Gynostemmae Pentaphylli Herba reversing Cervical intraepithelial neoplasia (CIN) were investigated by network pharmacology, and the mechanism of action was analyzed by molecular docking technology.</p><p><strong>Methods: </strong>The effective components and targets of Gynostemmae Pentaphylli Herba and the disease target of CIN were searched in TCMSP, Pubchem, Swiss Target Prediction, GenCards, WebGestalt, and STRING. Based on the above data and the Cytoscape software, we mapped the protein-protein interaction (PPI) co-expression network. The mechanism of Gynostemmae Pentaphylli Herba CIN treatment was identified from the enrichment analysis perspective. We performed molecular docking on the AutoDock. Finally, we carried out cell experiments for verification.</p><p><strong>Results: </strong>Eighty-five targets matching the active ingredients of Gynostemmae Pentaphylli Herba, and 2512 CIN-related action targets were obtained. The nodal degree values of five components and the target of Gynostemmae Pentaphylli Herba in the top 5 were IL6, IL1β, TNF, TP53, and PTGS2. There were 52 intersection targets of the effective active ingredient of Gynostemmae Pentaphylli Herba and CIN. The PPI network map suggested that the main active ingredient MOL000098 (Quercetin) had the most targets (40), followed by MOL000351 (Rhamnazin) (17). Gene Ontology (GO) analysis yielded the regulation of transcription from RNA polymerase II promoter, cytoplasm, extracellular space and enzyme binding, zinc ion binding, etc. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified 114 signaling pathways. These pathways were mainly enriched in positive regulation of transcription from RNA polymerase II promoter, positive regulation of gene expression, cytoplasm, extracellular space, enzyme binding, zinc ion binding, Pathways in cancer, Fluid shear stress and atherosclerosis, etc. The binding energies of IL6, IL1β, TNF, TP53, and PTGS2 with Quercetin, Rhamnazin, Gypenoside XXVII_qt and Gypenoside XXVIII_qt were all less than -5 kcal·mol<sup>-1</sup>, respectively. The messenger ribonucleic acid (mRNA) expressions of IL6, IL1β, TNF, and PTGS2 in the experimental group were higher (t = 105.700, 32.450, 18.190, and 100.400, all P < 0.001), and the mRNA expressions of TP53 was lower compared with the control group (t = 8.362, all P = 0.001).</p><p><strong>Conclusion: </strong>Gynostemmae Pentaphylli Herba may reverse CIN through targeted action of Quercetin, Rhamnazin, and gypenosides, as well as various pathways. Our study preliminarily explored the pharmacodynamic components and mechanism of the reversal of CIN by Gynostemmae Pentaphylli Herba and verified the possible mechanism of action through cell and molecular biology experiments to understand the pharmacological mechanism of Gynostemmae Pentaphylli Herba in CIN and provide data reference for new drugs and clinical trials research, whi","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"112"},"PeriodicalIF":3.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of MediYoga on sleep-quality, blood pressure and quality of life among older people with hypertension: study protocol of a pragmatic randomized controlled trial.","authors":"Signe Stelling Risom, Katrine Vollbrecht Amdi, Maria Wahlström, Trine Bernholdt Rasmussen, Suresh Sharma, Zarina Nahar Kabir, Hanne Konradsen","doi":"10.1186/s12906-025-04846-6","DOIUrl":"10.1186/s12906-025-04846-6","url":null,"abstract":"<p><strong>Background: </strong>High blood pressure (BP) is a common condition that is estimated to soon affect one third of the worlds' population. Poor subjective sleep quality is shown to be associated with an elevated risk of high BP, though it is a risk factor that can be modified. Yoga can be used as a complementary therapy to lower BP, but more knowledge on duration and intensity are needed. The overall aim is to test the effect of an online yoga intervention as a complementary therapy. The objectives are to: (1) Test whether online yoga can improve sleep quality, health related quality of life, and lower BP. (2) To explore and describe the implementation of online yoga and the participants' experiences and perspectives on the intervention in a process evaluation.</p><p><strong>Methods: </strong>A pragmatic randomized controlled trial will be conducted. The study is described in accordance with the SPIRIT guidelines (Standard Protocol Items: Recommendations for Interventional Trials) and results will be reported following the CONSORT (CONsolidated Standards Of Reporting Trials) guidelines for pragmatic trials. The study is designed as a three arm, randomized superiority trial.</p><p><strong>Inclusion criteria: </strong>≥65 years old, diagnosed with high BP, speaking and reading Danish, able to consent, no comorbidity that restricts them from participating in the intervention, and daily access to a smartphone or tablet. Participants will be randomly assigned to (i) control group which will be receiving treatment as usual (TAU), or to one of two intervention-groups (ii) performing yoga twice a week for 20 min (20 MIN) or (iii) 40 min (40 MIN) for a total of 10 weeks in addition to TAU. The primary outcome is sleep quality measured by the Pittsburg Sleep Quality Index and secondary outcomes include health related quality of life and BP. Analysis will present differences between groups and be carried out by a statistician blinded to group allocation.</p><p><strong>Discussion: </strong>The study is grounded in the urgent need to address high BP since pharmacological interventions remain the primary treatment modality, the exploration of non-pharmacological strategies, such as yoga, offers a promising avenue for enhancing patient outcomes in a holistic manner.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov ID NCT06553820. Protocol version 1. 13th November 2024.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"109"},"PeriodicalIF":3.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of single or multi-period use of transcutaneous acupoint electrical stimulation on postoperative nausea and vomiting in patients undergoing gynecological laparoscopic surgery: a prospective randomized double-blind trial.","authors":"Li-Dan Jin, Wan Lei, Jing Xu, Li Xing, Yao-Hua Shen, Su-Feng Lin, Yu-Fei Chen, Ting-Ting He, Xi-Yang Wang","doi":"10.1186/s12906-025-04847-5","DOIUrl":"10.1186/s12906-025-04847-5","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of different periods for the prevention of postoperative nausea and vomiting (PONV) in patients undergoing gynecological laparoscopy with transcutaneous electrical acupoint stimulation (TEAS).</p><p><strong>Design: </strong>Prospective, randomized, double-blind trial.</p><p><strong>Setting: </strong>An academic medical canter specializing in the care of women and children.</p><p><strong>Population or sample: </strong>A total of 120 women were enrolled.</p><p><strong>Methods: </strong>Patients were randomly allocated to three groups: a single-period TEAS group (Group S, n = 40), a multi-period TEAS group (Group M, n = 40) and a non-stimulation control group (Group C, n = 40). TEAS was applied at specific acupuncture points preoperatively and postoperatively.</p><p><strong>Main outcome measures: </strong>The primary outcome was the incidence and severity of PONV during the 48 h after surgery and the secondary outcomes included pain, early recovery after surgery, and complication.</p><p><strong>Results: </strong>Within postoperative 0-2 h, 4-12 h, group M had less frequency and lower scores for nausea compared with group C and group S had only less frequency of nausea compared with group C at 4-12 h postoperatively. The frequency and severity of vomiting for group M were less than group S in the postoperative 2-4 h. Group M mitigated pain and reduced the rescue antiemetic compared to group S.</p><p><strong>Conclusion: </strong>Multiple-period TEAS provides greater efficacy and a longer duration of action than single-period TEAS. It effectively reduces PONV in patients undergoing gynecological laparoscopic surgery which could be a new option in multimodal prophylactic antiemetic regimes for perioperative undergoing gynecological laparoscopic surgery.</p><p><strong>Trial registration: </strong>Chinese Clinical Trials Registry, No. ChiCTR2200065802, Registered 15/11/2022. https//www.chictr.org.cn/bin/project/edit? pid=175,377.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"110"},"PeriodicalIF":3.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a deep learning model for the automated monitoring of acupuncture needle insertion: enhancing safety in traditional acupuncture practices.","authors":"Shun-Ku Lin, Chien-Kun Su, Melnard Rome C Mercado, Syu-Jyun Peng","doi":"10.1186/s12906-025-04853-7","DOIUrl":"10.1186/s12906-025-04853-7","url":null,"abstract":"<p><strong>Background: </strong>Acupuncture is a widely practiced traditional therapy, yet safety concerns, particularly needle breakage and retention, remain critical issues that can lead to complications such as infections, organ injury, or chronic pain. This study aimed to develop a deep learning model to monitor acupuncture needle insertion, detect instances of needle breakage, and prevent needle retention, ultimately improving patient safety and treatment outcomes.</p><p><strong>Methods: </strong>A deep learning model based on the YOLOv8 architecture was trained using a dataset comprising 192 images from a commercial image library and 73 clinical images captured during real-world acupuncture sessions. Images were preprocessed through cropping and annotation, and augmented to enhance model generalizability. Five-fold cross-validation was employed to ensure robust performance. Model evaluation metrics included precision, recall, F1 score, and mean average precision (mAP) at Intersection over Union (IoU) thresholds of 50% (mAP@50) and 50-95% (mAP@50-95).</p><p><strong>Results: </strong>The model demonstrated strong performance, achieving an average precision of 88.0% and a recall of 82.9%. The mean average precision was 88.6% at mAP@50 and 62.9% at mAP@50-95, indicating high reliability in detecting acupuncture needles across diverse scenarios. These results highlight the potential of the model to enhance clinical safety by minimizing risks associated with needle breakage and retention, regardless of practitioner experience or patient demographics.</p><p><strong>Conclusions: </strong>The proposed YOLOv8-based deep learning model offers a reliable method for real-time needle monitoring in acupuncture. Its integration into clinical workflows can improve safety and efficiency, especially in underserved regions or settings with less experienced practitioners. Future research should validate the model with larger, more diverse datasets and explore its application in various healthcare settings.</p><p><strong>Trial registration: </strong>Not applicable; this study did not involve a healthcare intervention requiring registration. Data collection adhered to ethical standards with institutional approval (TCHIRB-11310004).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"108"},"PeriodicalIF":3.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the multi-target compounds of Sarcandra glabra identification and inhibition of novel target genes for the treatment of pancreatic cancer.","authors":"Xing Liu, Jianghong Ou","doi":"10.1186/s12906-025-04839-5","DOIUrl":"10.1186/s12906-025-04839-5","url":null,"abstract":"<p><strong>Background: </strong>S. glabra has been widely used to treat tumors in traditional Chinese medicine (TCM). However, the specific mechanism of action of S. glabra in pancreatic cancer remains unclear. In this study, network pharmacological analysis was used to identify the active components of S. glabra and their corresponding targets for the treatment of pancreatic cancer. Furthermore, molecular docking, molecular dynamic simulations, and in vitro experiments were performed to validate the findings.</p><p><strong>Methods: </strong>The active components of S. glabra and their corresponding targets for the treatment of pancreatic cancer were identified using the TCMSP database and a literature search. Differentially expressed genes were identified using data from the Gene Expression Omnibus (GEO) database, and their protein-protein interaction (PPI) network was constructed using the STRING platform. The target genes of S. glabra were further assessed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses in the R software. Subsequently, a protein-protein interaction (PPI) network and a composite target-pathway network were established. The target genes were subjected to survival and mutation analyses. Molecular docking and molecular dynamic simulations were used to validate the interaction between the hub target genes and S. glabra in vitro. In addition, cell viability and qRT-PCR verification of S. glabra against pancreatic cancer in vitro.</p><p><strong>Results: </strong>A total of 20 active components and 70 targets were identified. Based on the PPI network, CASP3, MMP9, CCND1, EGF, MMP2, CASP8, ERBB2, STAT1, and PPARG were identified as hub target genes. Enrichment analysis showed that S. glabra may primarily affect pathways such as p53 signaling, transcriptional dysregulation in cancer, proteoglycans in cancer, pancreatic cancer, and cell cycle. Molecular docking and molecular dynamic simulations indicated stable binding between anhydroicaritin-GSK3B and quercetin-PPARG. In vitro experiments demonstrated that treatment with S. glabra significantly inhibited the growth of PANC-1 cells and downregulated expression of GSK3B and PPARG (P < 0.05).</p><p><strong>Conclusion: </strong>This study demonstrates the potential of S. glabra, a herb in traditional Chinese medicine, for treating pancreatic cancer. The findings provide insights into the mechanism of action of the active ingredients of S. glabra, offering a strong theoretical foundation for its various clinical applications.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"106"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceptability and feasibility of online delivery of chair-based yoga for older adults with multimorbidity - lessons from a process evaluation of the gentle years yoga trial.","authors":"Lesley Ward, Laura Bissell, Jenny Howsam, Garry A Tew, Laura Wiley, Fiona Rose, Camila Sofía, Maturana Palacios, Tim Rapley","doi":"10.1186/s12906-025-04838-6","DOIUrl":"10.1186/s12906-025-04838-6","url":null,"abstract":"<p><strong>Background: </strong>Yoga is a safe, effective, and popular practice among older adults, and amenable to online delivery. The Gentle Years Yoga randomised controlled trial compared the impact of a chair-based yoga programme to usual care on the health-related quality of life of older adults with multimorbidity. This embedded, longitudinal process evaluation qualitatively explored experiences and acceptability of online delivery of the trial intervention.</p><p><strong>Methods: </strong>A subset of trial participants randomised to receive the 12-week online yoga programme, together with the trial yoga teachers, were purposively recruited to semi-structured interviews. Individual interviews were conducted via Zoom or telephone, audio-recorded, independently transcribed, and thematically analysed. Online observations were conducted of one class delivered by each teacher.</p><p><strong>Results: </strong>Eighteen yoga participants (66-91 years; 2-8 chronic health conditions) and nine teachers were interviewed once (N = 12) or twice (N = 15) from October 2020 to April 2022. Five themes predominated, common to both groups. (1) Accessibility. Reduced communication and engagement inherent to online delivery were mostly outweighed by its removal of access barriers and provision of anonymity and distraction-free environment. (2) Technology issues. While digital literacy was variable and a barrier for some, simplified access procedures and basic audiovisual instruction optimised class engagement. (3) Delivery adaptations. Key facilitation techniques included simple, repetitive instructions, increased demonstration, personalised communication, and visibility-enhancing clothing. (4) Safety. Concerns were minimal, and mostly related to restricted visual and positional information inherent to face-to-face classes. (5) Implications and implementations. Online delivery was considered viable and potentially appealing for anyone experiencing issues accessing face-to-face classes outside the home. Potential solutions to online attendance barriers included equipment loan schemes and digital learning courses using existing community-based infrastructures.</p><p><strong>Conclusions: </strong>Online chair-based yoga classes were feasible and acceptable to participants and teachers, and preferrable to face-to-face delivery by some. IT issues were minimal, and mainly resolvable through simple access processes and educational information. Accessibility advantages suggest online yoga may be suitable for a broad demographic, independent of age or health status. Establishing connections with existing health and community-based organisations presents a potential pathway for developing an equipment loan scheme to improve accessibility for those with financial access barriers.</p><p><strong>Trial registration: </strong>ISRCTN ISRCTN13567538. Registered 18 March 2019.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"107"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of garlic and stevia extract with aerobic exercise on hypothalamic leptin and ghrelin receptor mRNA expression and insulin resistance in obese rats.","authors":"Zohre Amirkhani, Armin Morteza Gholi, Sara Asghari, Danial Hakak, Mahdi Pouryousef, Behrooz Yahyaei, Sayyed-Javad Ziaolhagh","doi":"10.1186/s12906-025-04756-7","DOIUrl":"10.1186/s12906-025-04756-7","url":null,"abstract":"<p><strong>Background: </strong>Obesity has recently become the most prevalent diet-related disease worldwide. This study aimed to investigate the effects of aerobic exercise, stevia, and garlic extract on leptin and ghrelin receptor mRNA, as well as the interactions between insulin and glucose in relation to these genes.</p><p><strong>Methods: </strong>A total of 50 male Wistar rats were split into two groups: one group was fed regular rodent food, while the other was fed a high-fat diet for 12 weeks to induce obesity. The obese rats were then divided into six groups of five (N = 5) based on Lee's index: an obese control group (OC), obese treated with garlic, stevia (OGS), obese aerobic exercise (OE), obese garlic and aerobic exercise (OGE), Obese stevia and aerobic exercise (OSE), and a Normal weight group (NC). The exercise groups participated in a gradually increasing aerobic walking program, whereas the stevia and garlic groups received daily oral doses of their respective extracts. The expression of leptin and ghrelin receptor genes in the hypothalamus was measured using real-time PCR, and insulin resistance was calculated using the HOMA/IR formula. Statistical analysis was conducted using ANOVA, with a significance level set at P < 0.05.</p><p><strong>Results: </strong>The obese (O) rats experienced a significant increase in body weight compared with the control (C) rats (p = 0.013), whereas the treated rats showed no significant changes in weight. Leptin receptor mRNA levels were significantly lower in O rats than in C rats (p = 0.00), but increased significantly in the OS (p = 0.000) and OSE (p = 0.034) groups compared with obese rats. Ghrelin receptor mRNA levels were significantly higher in the O group than in the C group (p = 0.035), but decreased in all treatment groups, with the OE (p = 0.001), OGE (p = 0.001), and OSE (p = 0.004) groups showing the greatest reductions. Insulin resistance increased slightly in the O group compared with the C group (p = 0.112), but was lowest in the OS group among all groups (p = 0.018).</p><p><strong>Conclusions: </strong>Stevia significantly improved leptin and ghrelin receptor mRNA expression, glucose levels, and insulin resistance in obese rats, showing its potential as an effective dietary intervention for managing obesity.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"104"},"PeriodicalIF":3.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, antioxidant, antimicrobial, and anticancer activities of Mahonia napaulensis DC. bark from Nepal.","authors":"Rajib Kumar Shrestha, Satyabrata Nayak, Prabal Singh Maharjan, Bivek Pokharel, Gagan Deep Jhingan, Rana Pratap Singh, Neel Sarovar Bhavesh, Bhupal Govinda Shrestha","doi":"10.1186/s12906-025-04806-0","DOIUrl":"10.1186/s12906-025-04806-0","url":null,"abstract":"<p><strong>Background: </strong>Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or fewer side effects. This study aimed to assess total phenolic (TPC) and flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal, and anticancer), and chemical composition of methanol extract of M. napaulensis DC. bark (MNBM). This is the first study evaluating its anticancer activity and chemical composition by LC-MS/MS analysis.</p><p><strong>Methods: </strong>TPC, TFC, antioxidant, antimicrobial, and anticancer activities were determined by Folin-Ciocalteu, AlCl₃, DPPH, Resazurin, and MTT assays, respectively. Its metabolite profiling was done by LC-MS/MS analysis. The statistical significance of differences between test groups was analyzed by a one-way ANOVA.</p><p><strong>Results: </strong>The preliminary phytochemical screening revealed the presence of various phytochemicals viz. alkaloids, steroids, glycosides, polyphenols, tannins, flavonoids, coumarins, terpenoids, and quinone. MNBM showed 38.00 ± 1.50 mg GAE g^-1 dry sample as TPC, 35.04 ± 4.87 mg QE g^-1 dry sample as TFC, and 212.97 μg/mL IC₅₀ value (P < 0.05) as moderate antioxidant activity. MNBM showed minimal inhibitory concentration (MIC) values of 100.22 mg/mL, 50.15 mg/mL, and 25.08 mg/mL against S. aureus, E. coli, and C. albicans, respectively as weak antimicrobial activity. It showed no antibacterial effect against B. cereus and P. aeruginosa at 120 mg/mL. The anticancer activity of MNBM was moderate against human lung cancer cells A549 (228.97 μg/mL IC₅₀ value) and human cervical cancer cells HeLa (367.72 µg/mL IC₅₀ value) (P < 0.05). The LC-MS/MS analysis reported the presence of different anticancer compounds viz. dihydroberberine, d-berbamine, (S)-glaucine, protopine, grosheimin, mycophenolic acid, berberine, alpha-linolenic acid, etc. CONCLUSIONS: MNBM showed dose-dependent moderate antioxidant, weak antibacterial, weak antifungal, and moderate anticancer activity due to the synergistic effect of different phytochemicals and anticancer compounds.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"105"},"PeriodicalIF":3.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of Melissa officinalis L. essential oil inhalation on anxiety and symptom burden of hemodialysis patients: a randomized trial study.","authors":"Nilofar Pasyar, Mansoureh Aghababaei, Masoume Rambod, Mohammad M Zarshenas","doi":"10.1186/s12906-025-04840-y","DOIUrl":"10.1186/s12906-025-04840-y","url":null,"abstract":"<p><strong>Background: </strong>Hemodialysis patients suffer from physical and mental symptoms for which aromatherapy might be useful. This study aimed to determine the effectiveness of Melissa officinalis L. (Lemon Balm) essential oil inhalation on anxiety and the burden of symptoms in hemodialysis patients.</p><p><strong>Methods: </strong>This study is a randomized clinical trial with a pretest-posttest design. Sixty-eight hemodialysis patients were randomly divided into intervention (who underwent Melissa officinalis L. inhalation aromatherapy 3 times a week for one month) and control (who inhaled refined sweet almond oil) groups. The intervention in both groups was conducted during the hemodialysis session. The data were collected using the state-trait anxiety inventory and Dialysis Symptom Index. Data were analyzed through SPSS-25, using Mann-Whitney, Wilcoxon, t-tests and MANOVA.</p><p><strong>Results: </strong>Before the intervention, both groups were similar in terms of anxiety and symptoms burden (P > 0.05). However, after the intervention, a significant difference was found between the groups in terms of state and trait anxiety, and severity of constipation, nausea, vomit, diarrhea, swelling in legs, muscle cramps and shortness, worry, nervousness and anxiety, and trouble staying asleep. Moreover, after the intervention, a significant difference was observed between the groups regarding the prevalence of symptoms burden such as feeling anxious and having a dry mouth (P < 0.05).</p><p><strong>Conclusion: </strong>Melissa officinalis L. essential oil inhalation aromatherapy reduced the symptoms burden and anxiety in hemodialysis patients. Given the effectiveness of aromatherapy in hemodialysis patients, it is suggested that healthcare workers should use this complementary and integrative health to reduce the anxiety and symptoms burden in hemodialysis patients.</p><p><strong>Trial registration rct registry: </strong>Iranian Registry of Clinical Trials (IRCT) number: IRCT20191021045178N3; Registration date 27/04/2021.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"103"},"PeriodicalIF":3.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-reported therapeutic benefits of herbal medicinal products in the treatment of gynecological ailments.","authors":"Alexandra Drebka, Annika J Scholl, Teresa Ochs, Olaf Kelber, Ralph Mösges, Esther Raskopf, Kija Shah-Hosseini, Beatrice E Bachmeier","doi":"10.1186/s12906-025-04761-w","DOIUrl":"10.1186/s12906-025-04761-w","url":null,"abstract":"<p><strong>Background: </strong>Gynecological ailments have a negative impact on quality of life and productivity. Standard treatment is associated with poor tolerability and other issues related to public health and environment. Herbal Medicinal Products (HMPs) are used traditionally for the treatment of menstrual and menopausal ailments as well as uncomplicated urinary tract infections (uUTIs) for centuries and constitute a suitable addition to current treatment options. HMPs are well tolerated, non-polluting and therapeutically efficacious as evidenced by various clinical studies. Aim of this study was to expand the evidence regarding therapeutic effectiveness of HMPs for the treatment of gynecological complaints by complementing knowledge from clinical studies with real-world evidence from patient-reported outcomes.</p><p><strong>Methods: </strong>A data set consisting of patient-reported outcomes regarding the treatment of gynecological ailments (n = 1658) with HMPs was taken from the pharmaco-epidemiological database PhytoVIS. After data preparation excluding all cases of herbal supplements, homeopathic preparations, or non-herbal medicinal products the remaining data (n = 1363) was grouped into the three indications menstrual complaints (n = 222), menopausal complaints (n = 301), and uUTIs (n = 840). We applied descriptive statistical methods (frequency and percentage) with regard to the variables \"age\", \"treatment duration\", \"severity of symptoms\", \"therapeutic benefits\", and \"adverse drug reactions\". Thereafter we evaluated the therapeutic benefit of HMPs as well as adverse events.</p><p><strong>Results: </strong>The majority of the patients (82.2%) in the sample assessed the overall therapeutic effect of HMPs for the treatment of gynecological complaints as beneficial and 90.8% of them perceived no or no significant adverse events. Treatment habits differed depending on the type of complaint. In this context the majority of women with menstrual or menopausal ailments preferred to treat for time period of 1 month or longer, while those affected by uUTIs reduced the application of HMPs to the length of their symptoms. Interestingly women with even strong symptoms relied on the therapeutic benefit of HMPs.</p><p><strong>Conclusion: </strong>Real-world outcome data are an important supplement to clinical data. Our results reveal a favorable benefit-risk ratio of HMPs and help to implement them into novel therapeutic strategies to treat gynecological complaints.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"99"},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}