BMC Complementary Medicine and Therapies最新文献

{"title":"Evaluation of subchronic oral dose toxicity of Antrodia camphorata mycelium powder in rodents as a novel food source.","authors":"Won-Yong Shim, Eun-Sung Park, Sung-Il Jang, Shingo Horii, Wu-Che Wen, Sun-Don Kim","doi":"10.1186/s12906-026-05425-z","DOIUrl":"10.1186/s12906-026-05425-z","url":null,"abstract":"<p><p>Antrodia camphorata (A. camphorata), also known as \"Niuchangchih\" in Taiwan, is a medicinal fungus traditionally used for its hepatoprotective, anti-inflammatory, and anticancer properties. However, the safety and potential toxic effects of its mycelium form have not yet been fully established. This study aimed to evaluate the subchronic oral toxicity and determine the no observed adverse effect level (NOAEL) of A. camphorata mycelium powder in Sprague Dawley (SD) rats under Good Laboratory Practice (GLP)-compliant conditions.SD rats (10/sex/group) were administered A. camphorata mycelium powder orally at doses of 0, 500, 1000, and 2000 mg/kg/day as a 10 ml/kg/day suspension in water for 13 weeks. A 4-week recovery study was conducted at 0 and 2000 mg/kg/day with 5 rats/sex/group.Dose-dependent increases in liver and adrenal weights were noted, accompanied by hepatocellular and adrenal cortical hypertrophy, which were fully reversed in males and showed a tendency toward recovery in females after the recovery period. Repeated oral administration of A. camphorata mycelium powder for 13 weeks produced no adverse systemic toxicity in rats. The NOAEL was determined to be 2000 mg/kg/day in both sexes, supporting its safety for further development as a functional food or medicinal product. These findings provide important safety data supporting its further application in health supplements.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148161867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentials and challenges of an integrative medicine protocol in intensive care: the perspective of intensive care nurses.","authors":"Ilana Berlowitz, Eliane Timm, Jana Ertl, Ottilia Rohrer, Ursula Wolf","doi":"10.1186/s12906-026-05423-1","DOIUrl":"10.1186/s12906-026-05423-1","url":null,"abstract":"<p><strong>Background: </strong>Patients admitted to intensive care (IC) settings are exposed to a set of inevitable environmental stressors, such as high noise levels, inadequate lighting conditions, long-term immobilization, and prolonged administration of psychopharmaceuticals, the combination of which is known to be associated with clinical challenges, including poor sleep quality, dysregulation of circadian rhythms, agitation, anxiety, disorientation, or delirium. Due to the great complexity of the IC setting, these conditions are difficult to address with conventional means. The goal of the current study was to assess from the perspective of IC nurses the implementation of an integrative medicine approach based on topically applied herbal medicine substances to attend to some of these challenges. External applications of for instance Rosmarinus officinalis, Oxalis acetosella, and other herbal substances can have beneficial effects on peripheral circulation, sleep cycles, and digestion; they are considered safe with little drug interactions, hence presenting a valid candidate for the IC context.</p><p><strong>Methods: </strong>Employing a qualitative research approach, we used a series of focus groups with a sample of N = 49 specialized IC nurses working at the IC department of the University Hospital Bern, Switzerland. The qualitative data was analyzed using computer-assisted thematic analysis.</p><p><strong>Results: </strong>Four main theme clusters, each consisting of various sub-clusters, emerged from the analysis. Overall, participants considered the integrative medicine methods a valuable expansion to their therapeutic toolkit, as they offer additional, readily available, non-invasive, and low risk care options they could apply at their own discretion for their often greatly challenged patients. They described favorable experiences, but also reported barriers, like time constraints, or ineffective operational procedures and offered concrete suggestions on how to improve these.</p><p><strong>Conclusions: </strong>Taken together, while not assessing clinical effects, this work suggests tailored topical herbal medicines were perceived by IC nurses as feasible and acceptable complementary care options in selected situations.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148154887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated strategy for screening Q-markers and elucidating anti-fibrotic mechanisms of Ganoderma lucidum.","authors":"Yuanyuan Sun, Shujie Yuan, Wenhui Luo, Jie Wang, Renting Cao, Meng Su, Chijing Zuo, Can Peng, Jing Zhang","doi":"10.1186/s12906-026-05419-x","DOIUrl":"10.1186/s12906-026-05419-x","url":null,"abstract":"<p><strong>Background: </strong>Ganoderma lucidum (G. lucidum) is a medicinal mushroom known for its hepatoprotective effects against various liver diseases.</p><p><strong>Objective: </strong>Addressing the variable quality of G. lucidum samples and the lack of efficacy-linked quality control criteria, an integrated strategy combining chemical analysis and biological validation was conducted to identify the Q-markers directly responsible for its anti-hepatic fibrosis (HF) activity and to preliminarily elucidate their action mechanism.</p><p><strong>Methods: </strong>49 batches of G. lucidum samples were collected from 15 geographical origins across 7 provinces in China. Initially, UPLC fingerprinting integrated with chemometric analysis evaluated the quality and identified differential chemical components. The common compounds were further characterized by UPLC-Orbitrap-HRMS. Subsequently, the potential targets and binding affinity were predicted by network pharmacology and molecular docking. Finally, the anti-HF activity and underlying mechanism of the core Q-marker were verified in a TGF-β1-induced HSC-T6 model using CCK-8, flow cytometry, immunofluorescence, and Western blotting.</p><p><strong>Results: </strong>The UPLC chemical fingerprint of G. lucidum was successfully established, 49 common components were identified based on UPLC-Orbitrap-HRMS analysis, mostly were triterpenoids. Through chemometric analysis, 19 key differential markers were subsequently screened. Then, by integrating network pharmacology predictions with chemometric screening results, 7 triterpenoids were ultimately determined as potential Q-markers for the anti-HF activity of G. lucidum. Ganoderic acid J (GAJ) demonstrated robust binding specificity to pivotal targets within the PI3K/AKT signaling pathway through molecular docking. 5, 10, and 20 µg/mL at 24 h were confirmed as low, medium, and high doses of GAJ by CCK-8, respectively. Ultimately, HF was relieved by suppressing the PI3K/AKT pathway, promoting apoptosis in activated HSCs and normalizing the MMP1/MMP2 equilibrium.</p><p><strong>Conclusion: </strong>The anti-HF effects of G. lucidum were mediated through multi-target synergistic actions, chiefly by suppressing the PI3K/AKT pathway and modulating extracellular matrix metabolism. The hepatoprotective mechanism of G. lucidum was elucidated at the molecular level, and a Q-marker-based theoretical framework was established for quality control of traditional medicines and innovative drug development.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13404587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilobalide increases SK_Ca current via 5-HT_2BR-PLC-PKC pathway, causing hyperpolarization and enhanced EET production in human coronary artery endothelial cells.","authors":"Katesirin Ruamyod, Patcharaporn Taokum, Neennara Wongsatan, Sompol Tapechum, Wattana B Watanapa","doi":"10.1186/s12906-026-05418-y","DOIUrl":"10.1186/s12906-026-05418-y","url":null,"abstract":"<p><strong>Background: </strong>Ginkgo biloba extract and its active component bilobalide induce endothelium-dependent vasodilation in coronary and other vascular beds. This effect is associated with increased Ca²⁺-activated K⁺ channel activity in endothelial cells. We aimed to identify ion channels targeted by bilobalide in human coronary artery endothelial cells (HCAECs) and to explore the underlying mechanisms.</p><p><strong>Methods: </strong>Whole-cell patch-clamp recordings were performed on cultured HCAECs. Epoxyeicosatrienoic acids (EETs) and prostacyclin were quantified with enzyme-linked immunosorbent assays; nitric oxide and hydrogen peroxide were assessed with commercial kits.</p><p><strong>Results: </strong>Bilobalide dose-dependently increased whole-cell current through activation of the small-conductance Ca²⁺-activated K⁺ (SK_Ca) channel, but not through Cl^-, transient receptor potential, or other K⁺ channels. The current increase persisted when all channels except SK_Ca were blocked and was abolished by apamin, a selective SK_Ca blocker; additionally, apamin prevented bilobalide-stimulated current. Bilobalide also induced HCAEC hyperpolarization, which was inhibited by apamin. The bilobalide-induced current increase was blocked by antagonists of 5-hydroxytryptamine (5-HT)_1/2 and 5-HT_2B receptors, phospholipase C, and protein kinase C, but not by inhibitors of 5-HT_1B receptors, cytochrome P450, or G_i/o proteins. Furthermore, bilobalide stimulated EET production but not nitric oxide, prostacyclin, or hydrogen peroxide production; this effect was also apamin-sensitive.</p><p><strong>Conclusions: </strong>Bilobalide increases HCAEC current and induces hyperpolarization by activating SK_Ca via the 5-HT_2B receptor-phospholipase C-protein kinase C signaling pathway, leading to EET generation. These findings provide insights into bilobalide's endothelial mechanisms and highlight its potential as an alternative therapy for coronary artery disease.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13404313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The micro-phenomenology of Floatation-REST.","authors":"Helena Hruby, Marc Wittmann, Stefan Schmidt, Prisca R Bauer","doi":"10.1186/s12906-026-05415-1","DOIUrl":"10.1186/s12906-026-05415-1","url":null,"abstract":"<p><p>This micro-phenomenological study examines altered states of consciousness (ASC) during Floatation-REST, a sensory isolation method where individuals lie in a tank filled with Epsom salt-saturated water in complete silence and darkness. Salubrious effects of Floatation-REST have been shown in persons suffering from mental health conditions; these positive outcomes might be related to the experience of ASC. Micro-phenomenological interviews were conducted with fourteen participants who completed a 60-minute Floatation-REST session, to capture the generic structure of ASC. We found that most participants experience four distinct phases during a session (diachronic structure): after an initial acclimatization phase (1), participants experience a transitional phase (2) that prepares them for a subsequent ASC phase (3), followed by a phase of temporal and spatial reorientation (4). The ASC phase is characterized by the experience of positive emotions, deep relaxation, a loss of orientation in space and time, and a reduction in bodily sensations and thoughts (synchronic structure). The characteristics of the ASC phase emerge gradually and progressively intensify. This micro-phenomenological analysis of Floatation-REST sheds new light on the potential of this therapeutic technique. We discuss possible future research and therapeutic applications of Floatation-REST.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13217781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsions of 1,8-cineole, and Eucalyptus globulus and Laurus nobilis essential oils: cytotoxic, antibacterial, and antioxidant activities.","authors":"Mahsa Shafiei, Elham Zarenezhad, Hiva Alipanah, Homa Karimian, Razieh Ranjbar, Mahmoud Osanloo","doi":"10.1186/s12906-026-05422-2","DOIUrl":"10.1186/s12906-026-05422-2","url":null,"abstract":"<p><p>Antimicrobial and anticancer drug resistance is an increasing concern, driving interest in plant-based nanoformulations. Here, we developed and compared nanoemulsions containing Eucalyptus globulus essential oil (EO), Laurus nobilis EO, and their main constituent, 1,8-cineole. Gas chromatography-mass spectrometry (GC-MS) revealed 1,8-cineole content of 49.5% in E. globulus EO and 37.9% in L. nobilis EO. Nanoemulsions were prepared by spontaneous emulsification and characterized for droplet size (dynamic light scattering) and zeta potential. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy qualitatively confirmed EO encapsulation within the nanoemulsions. Average droplet sizes were 172 ± 9 nm (E. globulus EO), 92 ± 5 nm (L. nobilis EO), 137 ± 7 nm (1,8-cineole), and 70 ± 12 nm (blank nanoemulsion). The corresponding zeta potential values were - 24 ± 1.2 mV, - 34 ± 0.7 mV, - 10 ± 0.7 mV, and - 6 ± 1.5 mV. The L. nobilis EO nanoemulsion had the strongest antioxidant activity (IC₅₀ = 342 µg/mL). The E. globulus EO nanoemulsion showed the greatest cytotoxicity against A-375 human melanoma cells (IC₅₀ = 171 µg/mL) and the most potent antibacterial effects against Escherichia coli (IC₅₀ = 762 µg/mL) and Staphylococcus aureus (IC₅₀ = 205 µg/mL). These results suggest that E. globulus EO nanoemulsions have potential as antimicrobial and cytotoxic agents, pending confirmation in in vivo studies.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13397676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnobotanical documentation and conservation assessment of medicinal plants in the Bojanala District, North-West Province, South Africa.","authors":"Maropeng Erica Matlala, Chauke Sinorita, Elizabeth Kola, Luambo Jefferey Ramarumo, Peter Tshepiso Ndhlovu","doi":"10.1186/s12906-026-05396-1","DOIUrl":"10.1186/s12906-026-05396-1","url":null,"abstract":"<p><strong>Background: </strong>Bojanala District in North-West Province of South Africa is home to a diverse array of plant species, many of which are known for their medicinal properties. Despite their historical use, the pharmacological properties of these plants remain largely unexplored. The current study aimed to explore the phytomedicinal practices in Bojanala District by documenting medicinal plants and the associated indigenous knowledge.</p><p><strong>Methods: </strong>An ethnobotanical survey was conducted across six communities within the district between October 2024 and March 2025. Data were collected from 37 indigenous knowledge holders via face-to-face interviews using semi-structured questionnaires. The study followed purposive and snowball sampling methods. Data was then analyzed using ethnobotanical indices including Use Value (UV), Fidelity Level (FL), and Plant Part Value (PPV).</p><p><strong>Results: </strong>A total of 51 plant species from 28 families were documented, with Asteraceae (7 species) and Fabaceae (6 species) most represented. The most cited species were Artemisia afra (UV = 1.108), Dicoma anomala (0.622), Siphonochilus aethiopicus (0.459), Drimia sanguinea (0.459), and Hypoxis hemerocallidea (0.405). Twenty species, including Artemisia afra and Bidens pilosa, had an FL of 100%. Leaves (PPV = 0.329), roots (0.237), and bark (0.118) were the most used parts, primarily prepared as decoctions (54%). For example, Musculoskeletal conditions were treated with 20 species. Although 63% of species are Least Concern, some are declining due to overharvesting, trade, and habitat loss.</p><p><strong>Conclusion: </strong>Bojanala District retains extensive indigenous knowledge of medicinal plants, crucial for primary healthcare. Given population declines in some species, continuous monitoring, sustainable harvesting, and pharmacological validation of key species are recommended to support traditional use and guide the development of plant-based therapeutics.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13404135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The integration of network pharmacology and multiomics reveals the mechanism by which Malus hupehensis leaves inhibit acute liver injury.","authors":"Jia-Xin Qiao, Shu-Jie Lv, Bing-Tao Zhai, Xiu-Lin Zhang, Jiang-Xue Cheng, Jing Sun, Xiao-Fei Zhang, Dong-Yan Guo","doi":"10.1186/s12906-026-05409-z","DOIUrl":"10.1186/s12906-026-05409-z","url":null,"abstract":"<p><strong>Objective: </strong>The leaves of Malus hupehensis (MH) are a medicinal and edible TCM with good liver protection, although its underlying mechanism remains unclear. In this study, a network pharmacology-integrated multiomics method was used to explore the hepatoprotective mechanism of MH.</p><p><strong>Methods: </strong>The in vivo and in vitro components of MH were identified using ultra-performance liquid chromatography coupled with Q Exactive Orbitrap mass spectrometry (UPLC-Q Exactive Orbitrap-MS), and the targets and related pathways through which MH inhibits acute liver injury (ALI) were predicted by network pharmacology. The liver protective potential of MH was evaluated in a carbon tetrachloride-induced ALI model in C57BL/6J mice. The mechanism of MH was explored by integrating network pharmacology and multiomics and verified by immunohistochemistry, Western blot and RT‒qPCR analyses.</p><p><strong>Results: </strong>Our analysis revealed 121 in vitro and 20 in vivo components of MH. The network pharmacology analysis showed that the prototype components of MH, phlorizin and phloretin, were involved in inflammation and metabolism-related pathways. In vivo experiments revealed that MH significantly inhibited carbon tetrachloride-induced ALI in mice through antioxidant and anti-inflammatory effects. Combined network pharmacology and multiomics analyses revealed that MH protected the liver by inhibiting the activation of the MAPK signaling pathway and regulating metabolic reprogramming, specifically with respect to lipid and amino acid metabolism.</p><p><strong>Conclusions: </strong>Our study suggests that MH exerts a protective effect on carbon tetrachloride-induced ALI in mice, potentially by inhibiting MAPK signaling and regulating lipid and amino acid metabolic reprogramming. These findings provide a preliminary basis for exploring MH as a potential candidate for the prevention and treatment of ALI.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13393479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the knowledge, attitude, and practice of Egyptian pharmacists on probiotics: a cross-sectional study.","authors":"Noha S El Baghdady, Mohamed M Elmazar, Marwa S Hamza","doi":"10.1186/s12906-026-05406-2","DOIUrl":"10.1186/s12906-026-05406-2","url":null,"abstract":"<p><strong>Background: </strong>Probiotics, live microorganisms offering health benefits, have gained attention for their potential in preventing various health conditions. Despite their availability, usage remains limited, possibly due to a lack of understanding among healthcare professionals. This study assesses the knowledge, attitude, and practice (KAP) of Egyptian pharmacists regarding probiotics.</p><p><strong>Methods: </strong>A descriptive, cross-sectional study was conducted using a self-administered questionnaire to evaluate the KAP of Egyptian pharmacists concerning probiotics. Data were collected using a convenient sampling method. The questionnaire, developed after a literature review and pilot-tested for reliability, included sections on demographics, knowledge, attitudes, practices, and barriers to recommending probiotics.</p><p><strong>Results: </strong>Only 11% of pharmacists demonstrated good knowledge of probiotics, while 82% exhibited a positive attitude. However, just 3% reported good practice in recommending probiotics. Younger pharmacists (18-24 years) and those with less experience (0-4 years) showed poorer knowledge and attitudes. In contrast, older pharmacists (45-54 years) and those with more experience (10-14 years) had better knowledge and attitudes. Female pharmacists were more likely to have a positive attitude than males. Barriers to recommending probiotics included a lack of established clinical applications and insufficient knowledge.</p><p><strong>Conclusion: </strong>The study highlights significant gaps in the knowledge and practice of Egyptian pharmacists regarding probiotics, despite positive attitudes. Age, experience, and professional exposure are crucial factors influencing KAP. Enhanced educational resources and training are needed to improve pharmacists' understanding and integration of probiotics into healthcare practices.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13200401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yajieshaba extract improves alcohol‑induced liver injury by regulating hepatic lipid metabolism and gut microbiota.","authors":"Yang Liping, Yu Xingzhi, Tao Jie, Duan Xiaohua","doi":"10.1186/s12906-026-05421-3","DOIUrl":"10.1186/s12906-026-05421-3","url":null,"abstract":"<p><strong>Background: </strong>Yajieshaba, a commonly used Dai medicine formula, is renowned for its hepatoprotective properties. This study aimed to investigate the therapeutic effects and underlying mechanisms of Yajieshaba on alcoholic liver disease (ALD) in mice, focusing the gut-liver axis.</p><p><strong>Methods: </strong>Male C57BL/6 mice were pair-fed the Lieber-DeCarli control or ethanol-containing diet for 8 weeks, with or without Yajieshaba co-administration. Serum biomarkers were assessed using biochemical kits. Liver pathology was evaluated by hematoxylin and eosin (H&E) and Oil Red O staining. Intestinal barrier integrity was assessed by H&E staining and immunofluorescence of tight junction proteins (occludin, ZO-1). Hepatic lipid composition was analyzed by liquid chromatography-mass spectrometry (LC-MS), and gut microbiota diversity was profiled by 16 S rRNA sequencing.</p><p><strong>Results: </strong>Yajieshaba significantly attenuated ethanol-induced liver injury, steatosis, and intestinal barrier disruption. Multi-omics integration revealed that Yajieshaba mitigated ALD progression by restoring gut microbial homeostasis and regulating hepatic lipid metabolism.</p><p><strong>Conclusion: </strong>This study elucidates the therapeutic mechanism of Yajieshaba from the perspective of the gut microbiome-lipid metabolism axis, providing a novel perspective and experimental basis for further ALD management.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13378157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}