BMC Complementary Medicine and Therapies最新文献

{"title":"Raw and boiled ginger (Zingiber officinale Roscoe) inclusive diet improves fertility parameters in high cholesterol diet-induced obese rats.","authors":"Akomolafe Seun Funmilola, Atoyebi David Ayodeji","doi":"10.1186/s12906-025-04948-1","DOIUrl":"10.1186/s12906-025-04948-1","url":null,"abstract":"<p><strong>Background: </strong>Obesity has been implicated in reproductive problems, particularly in male functionality disorder while the therapeutic effects of ginger have been owing to its pharmacological activities.</p><p><strong>Aim: </strong>The study compares ginger-inclusive diet effects on fertility parameters in obese rats.</p><p><strong>Methods: </strong>Rats were given a high-cholesterol diet to induce obesity. The rats were split up into seven separate groups (n = 10): healthy control rats fed basal diet; obese untreated rats fed high cholesterol diet (HCD); reference drug-treated obese rats, clomiphene citrate (CC, 2 mg/kg BW/day); obese rats fed high cholesterol diet supplemented with 2% Raw Ginger (RG); obese rats fed high cholesterol diet supplemented with 4% RG; obese rats fed high cholesterol diet supplemented with 2% Boiled Ginger (BG); obese rats fed high cholesterol diet supplemented with 4% BG for twenty-four weeks.</p><p><strong>Results: </strong>A diet supplemented with raw and boiled ginger fed to obese rats increased adiponectin, estradiol, glycogen, enzymic and non-enzymic antioxidant levels with a concomitant reduction in leptin, lipid peroxidation and testicular cholesterol levels. Both raw and boiled ginger supplementation led to reductions in body weight of the obese rats. Furthermore, raw and boiled ginger improved sperm quality in obese rats by increasing sperm count, motility, viability and normality. Raw and boiled ginger also increased 3β and 17β-hydroxysteroid dehydrogenase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities as well as follicle-stimulating hormone, luteinising hormone and testosterone levels. In addition, both raw and boiled ginger increased Leydig cells and sperm cells while decreasing adipocyte size in the histological architecture of the testis, epididymis, and epididymal fat.</p><p><strong>Conclusion: </strong>This study found that both raw and boiled ginger-supplemented diets improved sexual function in obese rats by modulating metabolic hormones, sperm parameters, steroidogenic enzymes, and reproductive hormones, with the boiled ginger treatment outperforming the raw ginger treatment. As a result, we believe that boiled ginger, in addition to its effect on body weight regulation, may be useful in the treatment of obesity-induced male reproductive problems.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"209"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of radiation mediated testicular dysfunction by α-tocopheryl succinate: PPAR-γ related pathway.","authors":"Heba A Gheita, Ghada M Shafey, Maha M Aziz, Noha A Fadel","doi":"10.1186/s12906-025-04950-7","DOIUrl":"10.1186/s12906-025-04950-7","url":null,"abstract":"<p><strong>Background: </strong>Radiation exposure of sensitive organs during radiotherapy merits extraordinary consideration, particularly when the concern is about fertility. Although alpha-tocopheryl (vitamin E) is a potent antioxidant, many studies have demonstrated the radioprotective impact of alpha-tocopheryl acetate ester, emphasizing its antioxidant and anti-apoptotic effects; fewer studies were conducted using the succinate ester without any declaration of its anti-inflammatory effect in the concerned pathology. Accordingly, the current study was conducted to evaluate the dual antioxidant and anti-inflammatory role of alpha-tocopheryl succinate (α-TCS) in reproductive toxicity induced by gamma-irradiation.</p><p><strong>Methods: </strong>Animals were subjected to 6 Gy of whole-body gamma radiation and received α-TCS (200 mg/kg, P.O.) pre- and post-radiation. After the termination of the experiment, serum testosterone was estimated, and the testis weight was recorded. Besides, the testicular content of oxidative balance markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT)] and inflammatory response markers [interleukin-1β (IL-1β), nuclear factor-kappa B (NF-κB) p65, peroxisome proliferator-activated receptor-γ (PPAR-γ)] were assessed.</p><p><strong>Results: </strong>Irradiated (IR) rats showed disturbances in the testicular function and abnormal incidental lesions, as demonstrated in the histopathological examinations. They exhibited marked alterations in the testicular oxidative balance, verified by the rise of lipid peroxidation end product (MDA) and depletion of antioxidant enzymes (CAT and SOD). Also, radiation exposure triggered an inflammatory response, which was evidenced by suppression of PPAR-γ and intensified expression of NF-κB p65 subunit, with subsequent elevation in IL-1β testicular content. Conversely, administering α-TCS to IR rats maintained the testicular architecture and ultrastructure while also preserving testicular function. Treatment with α-TCS restored the oxidative balance (MDA, SOD and CAT) and reduced testicular content of pro-inflammatory cytokine IL-1β via interference with the NF-κB p65/ PPAR-γ signaling pathway.</p><p><strong>Conclusions: </strong>The current study sheds light on the crucial radioprotective role of α-TCS as a PPAR-γ agonist in maintaining testicular function partially through suppressing NF-κB activation and its downstream pro-inflammatory mediators.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"211"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional contraceptive practices: survey of medicinal plants used to control birth in four states in Nigeria.","authors":"Mutiat B Ibrahim, Akinniyi S Odewo, Bilqis A Lawal, Oluseye F Oyedeji, Noimot A Balogun, Wakilat A Tijani, Abdullahi S Abdulraheem, Jubril Shafiyi, Margaret O Ilomuanya","doi":"10.1186/s12906-025-04935-6","DOIUrl":"10.1186/s12906-025-04935-6","url":null,"abstract":"<p><p>Although modern contraceptive practices are actively advocated, many individuals continue to rely on traditional medicines for contraception due to their perceived safety. The open-ended questionnaire used in this study sought to gain insights into contraceptive practices from traditional providers' perspectives. Herb sellers and herbal practitioners from four yoruba-speaking states in Nigeria-Kwara, Lagos, Oyo, and Osun-were interviewed. Data from questionnaires were analyzed via descriptive and inferential statistics. Quantitative analysis was done using the informant consensus factor (ICF) and relative frequency of citation (RFC) indices. A total of 31 species were recorded across the study areas. Kwara had the highest diversity, with 20 plant species, followed by Lagos (16), Oyo (10) and Osun (4). The ICF value recorded across the study areas was 0.7, with the most frequently cited species for female and male contraception varying by location. Carica papaya had RFC values of 0.58 in Oyo and 0.44 in Osun, while Mucuna pruriens recorded 0.30 in Lagos and Jatropha gossypiifolia had 0.27 in Kwara. The plant species were mostly prepared in combination forms, with seeds and leaves being the most used parts. These were administered as herbal contraceptive decoctions, powders, or worn as contraceptive rings or waist beads. A statistically significant difference existed between the plant form, the education level of participants, and having patients seeking contraception. In conclusion, this survey underscores the diverse range of medicinal plant species used for contraception. Further research is essential to validate their efficacy and understand the mechanisms of herbal contraceptives.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"210"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-oxidative effect of Salvia miltiorrhiza Bunge fermented with Shiraia bambusicola Henn. against H₂O₂-induced injury in PC12 cells.","authors":"Qiaona Wang, Canhe Zhu, Zhaoran Song, Yunfa Qiao, Yuefeng Hu, Liyun Hu, Shengjie Li, Renlei Wang","doi":"10.1186/s12906-025-04956-1","DOIUrl":"10.1186/s12906-025-04956-1","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of this investigation was to produce fermented solid-state products of Salvia miltiorrhiza Bunge (SMB) utilizing Shiraia bambusicola Henn. (FSSMB). Additionally, it was intended to further explore toxicity-reducing potential and antioxidant effects of FSSMB ethanol extract against H₂O₂-induced oxidative stress in rat pheochromocytoma cell line 12 (PC12).</p><p><strong>Methods: </strong>PC12 cells were pre-treated with SMB or FSSMB ethanol extract for 24 h, followed by exposure to 1 mmol/L H₂O₂ for 3 h to induce oxidative stress. Subsequently, the cell viability, reactive oxygen species (ROS) level, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, p-p38/p38 and p-JNK/JNK proteins were assessed. Additionally, the content of antioxidant, including tanshinones (tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone) and salvianolic acids (danshensu, rosmarinic acid, lithospermic acid, and salvianolic acid B) were quantified by HPLC in SMB and FSSMB.</p><p><strong>Results: </strong>Here, we found that FSSMB exhibited lower cytotoxicity compared to SMB in PC12 cells, and FSSMB instead of SMB significantly increased cell viability in H₂O₂-induced PC12 cells. Notably, FSSMB exhibited superior antioxidant properties in H₂O₂-induced PC12 cells, as evidenced by reduced levels of ROS, MDA, and enhanced SOD activity compared to SMB. Mechanistically, FSSMB reversed H₂O₂-induced increase of phosphorylation of p38 and JNK protein in mitogen-activated protein kinase (MAPK) signaling pathway, thereby protecting PC12 cells from oxidative stress-induced injury. Furthermore, we found a significant increase of tanshinone IIA and cryptotanshinone content, accompanied by decreased levels of tanshinone I, dihydrotanshinone and salvianolic acids in FSSMB compared to SMB.</p><p><strong>Conclusions: </strong>Our study explores a new biological transformation approach through solid-state fermentation (SSF) with Shiraia bambusicola Henn. on SMB. This study demonstrated that the SSF may reduce the cytotoxicity and enhance the antioxidant capacity of SMB in PC12 cells, thus paving the way for safer and more efficacious applications in the future. FSSMB may be an effective substitute for traditional Chinese medicine SMB for resistance to oxidative stress.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"208"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of traditional medicine in providing palliative care and improving the quality of life: a survey on patients' knowledge and utilization in Kumasi metropolis, Ghana.","authors":"Antwi Joseph Barimah, Kofi Bobi Barimah, James Dumba, Yaw Boakye Nketiah, Adom-Brobbey David, Tampuri Sheena Mmaa, Asantewaa Nancy Ntow, Owusu Kwadwo","doi":"10.1186/s12906-025-04961-4","DOIUrl":"10.1186/s12906-025-04961-4","url":null,"abstract":"<p><strong>Background: </strong>According to the World Health Organization (WHO) the utilization of traditional medicine items exceeds that of conventional medications by two to three times on a global scale. Ghana's health system has seen numerous changes and revolutions since gaining political independence, but the nation still struggles to offer its rapidly expanding population an adequate, suitable, complete, and efficient healthcare system. This study assessed the role of traditional medicine in providing palliative care and improving the quality of life while focusing on patients' knowledge and utilization.</p><p><strong>Methods: </strong>A hospital-based descriptive cross-sectional study design was employed for the study. The study population consisted of patients who were receiving traditional medical treatment for health conditions such as diabetes and hypertension in the Kumasi metropolitan area. A multi-stage sampling technique was deployed in the study of which 440 participants made up the total sample.</p><p><strong>Results: </strong>The findings of the study revealed that a substantial proportion of patients (81.04%) demonstrated a strong knowledge of traditional medicine within the context of palliative care. Awareness of traditional medicine was universal among participants (100%), with herbal medicine being the most commonly recognized form (97.39%). Additionally, nearly all participants (99.53%) acknowledged the potential role of traditional medicine in palliative care and affirmed its accessibility (98.82%).</p><p><strong>Conclusion: </strong>Patients extensively used alternative medicine and showed strong contentment with its success, noting notable enhancements in their health. A lot of people suggest converting traditional medicine into modern dosage forms in order to improve health outcomes and quality of life.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"212"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and hepatoprotective effect of Jatropha dioica against the valproic acid-induced damage in an in vivo model.","authors":"Paula Cordero-Pérez, Ramiro Tijerina-Márquez, Veronica Mayela Rivas-Galindo, Liliana Torres-González, Diana Raquel Rodríguez-Rodríguez, Oscar Humberto Mendoza-Hernández, César Bigran Espinosa-Cantú, Guadalupe Yazmín Solís-Cruz, Linda E Muñoz-Espinosa, Edelmiro Pérez-Rodríguez, Idalia Cura-Esquivel, Gabriela Alarcón-Galván, Diana Patricia Moreno-Pena","doi":"10.1186/s12906-025-04914-x","DOIUrl":"10.1186/s12906-025-04914-x","url":null,"abstract":"<p><strong>Background: </strong>Understanding liver diseases is important worldwide due to their prevalence. Apart from liver disease arising from hepatitis C viral infection, most chronic liver diseases currently have no cure. Several therapeutic alternatives, including some natural products, have been proposed to treat liver diseases. The natural product Jatropha dioica has been reported to possess antioxidant activity and, by extension, could have hepatoprotective activity. Accordingly, our aim was to test the hypothesis that an extract of J. dioica is protective against liver damage induced by valproic acid (VPA).</p><p><strong>Methods: </strong>Twelve male and twelve female Wistar rats were sorted into four groups: control, non-toxicity, valproic acid control (VPA-C), and J. dioica + VPA (JdVPA). J. dioica (300 mg/kg, given orally) was used as treatment, followed by a concomitant injection of VPA (500 mg/kg, i.p.) for the first 4 days to induce liver injury. To evaluate the severity of the injury, liver function tests were performed. In addition, oxidative stress biomarkers were quantified, as well as measures of the expression of the genes Actb, Il6, and Nfkb1.</p><p><strong>Results: </strong>The VPA-C group showed a significant increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA), a decrease in superoxide dismutase (SOD), and a reduction in glutathione (GSH) vs the control group. The JdVPA group showed a significant decrease in ALT, AST, and MDA and an increase in GSH and SOD vs the VPA-C group. Gene expression of Il6 and Nfkb1 did not show any statistically significant differences between study groups. Histologically, VPA presented an inflammatory infiltrate, which decreased in the JdVPA group.</p><p><strong>Conclusion: </strong>The extract of J. dioica at the administered dose did not display toxicity and was capable of ameliorating the liver injury generated by VPA in biochemical and oxidative stress biomarkers, which suggests its potential hepatoprotective activity.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"207"},"PeriodicalIF":3.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotonin release mediates analgesia via opioidergic system and withdrawal symptoms in chronic kratom extract-treated mice.","authors":"Dwi Wahyu Indriani, Siti Irma Rahmawati, Asep Bayu, Peni Ahmadi, Anissa Nofita Sari, Zuraida Zuraida, Ni Luh Putu Indi Dharmayanti, Masteria Yunovilsa Putra","doi":"10.1186/s12906-025-04947-2","DOIUrl":"10.1186/s12906-025-04947-2","url":null,"abstract":"<p><strong>Background: </strong>Kratom alleviates pain by activating μu-opioid receptors (MOR), which trigger serotonin release to produce analgesia. Serotonin also interferes drug abuse effect. This study aimed to determine the role of serotonin in kratom-induced pain relief and withdrawal symptoms in mice.</p><p><strong>Methods: </strong>The analgesic effect was assessed using the hot-plate test. To induce withdrawal symptoms, mice received naloxone after being treated with kratom extracts for five days at increasing doses. Another group of morphine-dependent mice was treated with kratom extracts to ameliorate their withdrawal symptoms. A molecular docking study and molecular dynamics were conducted to predict the binding target of alkaloid kratom for increasing serotonin levels.</p><p><strong>Results: </strong>Chronic administration of kratom alkaloid extract (20 mg/kg) produced analgesic effects comparable to morphine (10 mg/kg). In contrast, kratom crude extracts (10 mg/kg and 20 mg/kg) demonstrated lower analgesia activity. This analgesic effect was mediated by MOR activation, leading to decreased intracellular cAMP and increased serotonin transmission. Repeated and increasing doses of crude or alkaloid kratom extracts (8 mg/kg to 45 mg/kg) produced less severe withdrawal symptoms than morphine. Increased dopamine and serotonin levels contributed to the onset of withdrawal symptoms. In the morphine group, treatment with kratom extracts increased serotonin levels while reducing dopamine. Molecular docking and molecular dynamics result revealed that kratom alkaloids interacts more readily with tryptophan hydroxylase, the enzyme responsible for serotonin biosynthesis.</p><p><strong>Conclusions: </strong>Kratom extracts have the potential to provide analgesic effects and withdrawal symptoms, both of which are mediated by elevated serotonin release.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"205"},"PeriodicalIF":3.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of binaural beat therapy with different frequencies on autonomic nervous system regulation among college students.","authors":"Shang-Yu Yang, Jiun-Yi Wang, Cheng Liu, Pin-Hsuan Lin","doi":"10.1186/s12906-025-04922-x","DOIUrl":"10.1186/s12906-025-04922-x","url":null,"abstract":"<p><strong>Background: </strong>Although binaural beat therapy (BBT) has been demonstrated to alleviate symptoms of anxiety and depression, but the effects of BBT of different frequencies on college students have not been thoroughly explored. This study explored the effects of BBT with different frequencies on autonomic nervous system regulation (i.e., anxiety reduction) among college students.</p><p><strong>Methods: </strong>This was a quasiexperimental study. Participants were recruited from a university in southern Taiwan. Each of the participants received interventions with different frequencies (theta frequency, 6 Hz; alpha frequency, 10 Hz; and beta frequency, 25 Hz). For each intervention, participants were subjected to 20 min of natural sounds embedded with binaural beats of a specific frequency. A pretest and posttest were conducted to measure blood pressure (BP) and heart rate variability.</p><p><strong>Results: </strong>A total of 65 participants were recruited. The mean age of participants was 20.15 years. Heart rate and systolic and diastolic BP were significantly lower after intervention with theta-frequency binaural beats (p < 0.05). Systolic BP was significantly lower after intervention with alpha-frequency binaural beats (p < 0.05). Heart rate, systolic BP, and nLF were significantly lower and nHF was significantly higher after intervention with beta-frequency binaural beats (p < 0.01). No significant differences were observed between the effects of the three interventions on ANS regulation among the participants.</p><p><strong>Conclusion: </strong>BBT, in which participants were subjected to theta-frequency, alpha-frequency, and beta-frequency binaural beats for 20 min, contributed to reducing anxiety. No significant differences were observed between the effects of each frequency.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"206"},"PeriodicalIF":3.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the neuroprotective mechanisms of Jiawei Suanzaoren decoction in depression: insights from network pharmacology and molecular docking.","authors":"Ruiting Ma, Wenjing Zhang, Lixia Chen, Lijun Tong","doi":"10.1186/s12906-025-04939-2","DOIUrl":"10.1186/s12906-025-04939-2","url":null,"abstract":"<p><strong>Background: </strong>Depression is a prevalent and debilitating neuropsychiatric disorder, often associated with neuroinflammation, oxidative stress, and neuronal apoptosis. Jiawei Suanzaoren (JWSZR), a traditional Chinese medicine (TCM) formulation, has demonstrated potential in alleviating depressive symptoms. However, its precise molecular mechanisms remain unclear. This study aims to elucidate the neuroprotective effects of JWSZR in depression using network pharmacology, molecular docking, and in vitro experimental validation.</p><p><strong>Methods: </strong>Active compounds of JWSZR were identified using the TCMSP and HERB databases, and depression-related targets were retrieved from GeneCards, DisGeNET, and OMIM. A protein-protein interaction (PPI) network was constructed, followed by functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Molecular docking was employed to predict the interactions between JWSZR's active components and key target proteins. Furthermore, in vitro experiments were performed using corticosterone (CORT)-induced PC12 cell model to validate the neuroprotective effects of JWSZR, assessing cell viability and apoptosis rates.</p><p><strong>Results: </strong>Network pharmacology and molecular docking revealed that JWSZR exerts neuroprotective effects through multiple targets, including estrogen receptor ESR2, HSP90AA1, and STAT1. These targets regulate immune responses, inflammatory pathways, and cell survival. In vitro, JWSZR significantly improved cell viability and reduced apoptosis in CORT-treated PC12 cells, indicating its potential to protect against depression-related neurodegeneration.</p><p><strong>Conclusion: </strong>This study provides novel insights into the neuroprotective mechanisms of JWSZR in depression, suggesting that it may act through multi-target interactions involving immune modulation and apoptosis inhibition.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"204"},"PeriodicalIF":3.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin.","authors":"Nihal Turkmen Alemdar, Selim Demir, Esin Yulug, Ali Kulaber, Elif Ayazoglu Demir, Nadire Sevdenur Erdogan, Ahmet Mentese, Yuksel Aliyazicioglu","doi":"10.1186/s12906-025-04944-5","DOIUrl":"10.1186/s12906-025-04944-5","url":null,"abstract":"<p><strong>Background: </strong>Acetamiprid (ACP) is a novel chloronicotinyl insecticide that has been extensively utilized in agricultural, domestic, and public health contexts for nearly two decades. However, its potential to induce organ damage, including reproductive toxicity in mammals, has emerged as a significant concern. Morin is a naturally occurring flavonol that has gained prominence as a food supplement in recent years due to its antioxidant and anti-inflammatory properties. The objective of this study was to evaluate the protective effect of morin against testicular damage in mice subjected to ACP exposure.</p><p><strong>Methods: </strong>Thirty male Balb/c mice were randomly assigned to one of five groups, with the following treatment allocations: control, ACP (20 mg/kg), ACP + morin (15 and 30 mg/kg), and only morin (30 mg/kg). ACP and morin applications were conducted orally over a period of 14 days. Hormonal analyses were conducted on serum samples obtained from the mice, while biochemical and histological evaluations were performed on testicular samples.</p><p><strong>Results: </strong>The biochemical results demonstrated that ACP elevated oxidative stress, inflammation, and ER stress in testicular tissue by inhibiting the Nrf2 pathway, a finding that was corroborated by histopathological analyses. However, morin treatments eliminated ACP-induced Nrf2 inhibition and to activate antioxidant and anti-inflammatory mechanisms. These findings were also corroborated by the restoration of serum testosterone and inhibin B levels and the diminution of histopathological lesions.</p><p><strong>Conclusions: </strong>Overall, the findings indicated that morin may have potential protective properties against ACP-associated reproductive toxicity, however, further research is required to determine the detailed molecular mechanisms.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"203"},"PeriodicalIF":3.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}