BMC Complementary Medicine and Therapies最新文献

{"title":"Effect of nipa palm (Nypa fruticans Wurmb.) vinegar on the incretin hormones and intestinal glucose transporters in type 2 diabetes mellitus rat model.","authors":"Nur Izzati Razali, Tri Widyawati, Dwi Rita Anggraini, Vuanghao Lim, Nor Adlin Yusoff","doi":"10.1186/s12906-025-04933-8","DOIUrl":"https://doi.org/10.1186/s12906-025-04933-8","url":null,"abstract":"<p><p>Nipa palm vinegar has been traditionally used to lower blood glucose levels by diabetic patients. This study aims to analyse the effect of aqueous extract (AE) of nipa palm vinegar on glycemic parameters and glucose transporter-incretin hormonal system in type 2 diabetic rat. The model was established using a combination of high-fat diet (p.o.) and low dose streptozotocin (i.p.). AE (250, 500, and 1000 mg/kg) was administered orally once daily for 28 days. Biochemical parameters related to type 2 diabetes including fasting glucose, serum insulin, lipid profiles, incretin hormone, liver, and pancreatic histology were evaluated. Relative expression of jejunal glucose transporters was also determined. Induction of diabetes caused significant (p = 0.026) weight loss, hyperlgycemia, hypoinsulinemia, dyslipidemia and reduced incretin hormones. Diabetes onset also disturbed HOMA-IR and HOMA-ß cell function indices, altered the morphological features of hepatocytes and pancreatic islet and overexpressed intestinal glucose transporters, SGLT1 and GLUT2. Repetitive oral administration of AE (1000 mg/kg) for 28 days ameliorated the biochemical abnormalities and improved HOMA-β cell function of diabetic rats. Histological studies revealed AE treatment preserved the integrity of pancreatic islet and protected hepatocytes from degeneration and atrophic effects of streptozotocin. Further analysis suggested the effect of AE in stimulating incretin hormones secretion via the action of DPP4 inhibitor and by modulating jejunal SGLT1 expression. In conclusion, the study suggested AE exerted its antidiabetic effect partially by stimulating insulin secretion via incretin hormone and intestinal glucose transporter pathway.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"192"},"PeriodicalIF":3.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experiences and perceptions of Chinese patients enrolled in a clinical trial assessing tuina and manual therapies for knee osteoarthritis: a nested qualitative study.","authors":"Luping Liu, Lingyun Zhang, Sina Li, Meiling Cai, Siyu Han, Zhiwen Weng, Qianji Chen, Yixuan Gao, Xiaoming Yang, Yang Zhang, Duoduo Li, Changxin Liu, Ya'nan Sun, Xiyou Wang, Changhe Yu","doi":"10.1186/s12906-025-04926-7","DOIUrl":"10.1186/s12906-025-04926-7","url":null,"abstract":"<p><strong>Introduction: </strong>KOA is a prevalent joint disorder significantly impacting patients' quality of life. Tuina and manual interventions are prioritized in clinical practice within the Chinese healthcare context. Current qualitative studies mostly focus on symptom management and basic disease perceptions, overlooking patient-centered treatment expectations, therapeutic process perceptions, and doctor-patient interaction impacts during manual therapy. This study aims to address these gaps by exploring Chinese KOA patients' experiences, perceptions, and expectations of manual therapy, emphasizing contextual factors affecting therapeutic outcomes and interactions.</p><p><strong>Methods: </strong>Participants with KOA were sampled using a simple sampling method from a randomized controlled trial of Tuina treatment versus manual physical therapy (MPT). The interviews were conducted by two researchers who have extensive experience interviewing KOA patients, and data were gathered through face-to-face, semi-structured interviews to ensure a high level of information power. Three experienced researchers subsequently analyzed employing thematic analysis to assess patient experiences and outcomes from both treatment modalities.</p><p><strong>Result: </strong>The study interviewed a total of 61 participants, thematic saturation was reached when interviewing 42 participants, and seven codes along with 5 sub-themes were utilized to depict potential doctor-patient interactions and influencing factors. This process led to the formation of three themes: Understanding and Impact, Treatment Expectations and Satisfaction, and Treatment Goals and Outcomes, which helped in constructing a model to understand the underlying influences among these themes.</p><p><strong>Conclusion: </strong>Our study generated three themes-Understanding and Impact, Treatment Expectations and Satisfaction, and Treatment Goals and Outcomes, and developed a manual therapy model based on these themes. The generated model shows the important factors of doctor-patient interaction in KOA manual therapy management. Future research should expand to multidisciplinary and cross-cultural models to align standardized protocols with individualized patient needs.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"191"},"PeriodicalIF":3.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated network pharmacology reveals the mechanism of action of Xianlinggubao prescription for inflammation in osteoarthritis.","authors":"Jingyi Hou, Yubo Li, Yu Zhang, Ning Yang, Bin Chen, Guiyun Ma, Naiqiang Zhu","doi":"10.1186/s12906-025-04928-5","DOIUrl":"10.1186/s12906-025-04928-5","url":null,"abstract":"<p><strong>Background: </strong>Osteoarthritis (OA), a leading cause of disability worldwide, is characterized by complex interactions between cartilage degradation and synovial inflammation. While NSAIDs are the primary treatment, their prolonged use exacerbates gastrointestinal risks and does not alter disease progression. Xianlinggubao (XLGB), an approved Chinese herbal remedy for osteoporosis, has demonstrated promising anti-osteoarthritic effects in preliminary studies. However, its multi-component mechanisms targeting OA-related inflammation require further clarification. This study integrates network pharmacology with experimental validation to investigate XLGB's anti-inflammatory mechanisms in OA.</p><p><strong>Methods: </strong>Bioactive compounds of XLGB and their respective targets were sourced from the TCMSP, ETCM, SymMap, and ChEMBL databases. Targets linked to OA-related inflammation were identified through differential expression analysis and by querying OMIM, GeneCards, and PubMed Gene databases. Network pharmacology and bioinformatics approaches were employed to construct compound-target and protein-protein interaction (PPI) networks, enabling the identification of pivotal therapeutic targets. Functional enrichment of these targets was performed using the ClusterProfiler package in R. The binding affinity of compounds to anti-inflammatory OA targets was assessed through molecular docking, dynamics simulations, RT-PCR, and immunofluorescence assays.</p><p><strong>Results: </strong>Fifty-five bioactive compounds corresponding to 475 XLGB targets and 125 genes involved in OA-related inflammation were identified. PPI network analysis revealed that XLGB may alleviate OA inflammation by modulating key genes, including COX-2, IL-1β, TNF, IL-6, and MMP-9. Molecular simulations indicated strong binding affinities between bioactive compounds in XLGB and these critical targets. Functional enrichment analysis suggested that XLGB's anti-inflammatory action in OA may involve regulation of pathways such as IL-17, TNF, and NF-κB. In vitro experiments further confirmed that XLGB mitigates OA inflammation by modulating these genes, proteins, and signaling pathways.</p><p><strong>Conclusions: </strong>Through network pharmacology, this study elucidated the mechanisms of XLGB in OA inflammation, highlighting its modulation of IL-6, IL-1β, TNF-α, PTGS2, MMP-9, and the NF-κB pathway. These findings provide strong support for the clinical application of XLGB in managing OA-related inflammation.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"190"},"PeriodicalIF":3.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometriosis-like lesions induced by phthalates: new phytotherapic applications to complement traditional cares: a PNRR 2023 project.","authors":"Roberta Tassinari, Massimo D'Archivio, Rosaria Varì, Beatrice Scazzocchio, Gabriele Lori, Maria Bellenghi, Annalisa Silenzi, Alessia Tammaro, Daniele Marcoccia, Valentina Tassinari, Antonella Smeriglio, Domenico Trombetta, Antonio Maiorana, Maria Clara Leone, Francesca Maranghi","doi":"10.1186/s12906-025-04913-y","DOIUrl":"10.1186/s12906-025-04913-y","url":null,"abstract":"<p><strong>Background: </strong>Endometriosis (E) is an oestrogen-dependent, multifactorial, inflammatory disease causing pelvic pain and infertility. Several concerns have been raised about the role of food contaminants, in particular Bis(2-ethylhexyl) phthalate (DEHP), potentially involved in the onset and propagation of E. Conventional therapies- which have considerable side effects - focus on reducing levels of oestrogens and counteracting inflammation. The potential preventive/protective role of plant extracts (PEs) on phthalate (PH) -induced E is studied by a stepwise approach.</p><p><strong>Methods: </strong>(i) raw material identification, extraction and phytochemical characterization; (ii) in vitro tests to evaluate pharmacokinetics and organotropism; (iii) in vitro screening on 2 human endometrial cell lines and in vivo toxicokinetic to select the PEs/BCs in comparison with non-steroidal anti-inflammatory drugs; (iv) in vivo juvenile toxicity study to test the PE/BC activity on DEHP induced E-like lesions and (v) ex vivo and in vitro studies on human E primary cells obtained by patients with E to be subjected to scheduled surgical procedures and human non-cancerous cells, to investigate the DEHP and metabolite concentration and PE/BC effects, respectively.</p><p><strong>Discussion: </strong>The project aims to provide data and tools to develop a new strategy based on herbal medicine- especially polyphenolic compounds for their pleiotropic activities - to mitigate the E symptoms and to prevent and/or to protect population - including susceptible sub-groups - from the onset of E. The outcomes of the project will support the Italian National Health System in the development of complementary alternative/preventive strategies for E and to set clinical studies on humans also considering the potential role of environmental contaminants in E pathogenesis.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"188"},"PeriodicalIF":3.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profiling and anticancer activity of Alnus incana dichloromethane fraction on HeLa cells via cell cycle arrest and apoptosis.","authors":"Walaa Hesham, Emad M Elzayat, Mohamed Hosney, Fatma Abo-Elghiet","doi":"10.1186/s12906-025-04920-z","DOIUrl":"10.1186/s12906-025-04920-z","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer remains a global health challenge with persistently high incidence and mortality rates despite advancements in conventional treatments. The therapeutic potential of natural products has gained attention, particularly for their selective cytotoxicity and ability to modulate cancer pathways. Alnus incana (L.) Moench, a species-rich in bioactive compounds, shows potential as an anticancer agent; however, the cytotoxic effects of its leaves dichloromethane (DCM) extract remain underexplored. This study investigates the DCM fraction's cytotoxicity on various cancer cell lines, with a primary focus on HeLa cells.</p><p><strong>Methods: </strong>The cytotoxic effects of the A. incana DCM fraction were evaluated in a dose-dependent manner using the MTT assay on several cancer cell lines, with particular emphasis on HeLa cells. Flow cytometry was used to assess cell cycle arrest and apoptosis, while RT-qPCR quantified changes in the expression of apoptotic markers (Bax, Bcl-2, and p53). Chemical composition analysis was conducted using gas chromatography-mass spectrometry/flame ionization detection (GC-MS/FID) to identify the major bioactive compounds within the fraction.</p><p><strong>Results: </strong>The DCM fraction exhibited dose-dependent cytotoxicity in HeLa cells, with an IC₅₀ value of 135.6 µg/mL and a selectivity index (SI) of 2.72 relative to normal cells. Flow cytometry analysis revealed G0/G1 cell cycle arrest, significantly hindering progression through the S and G2/M phases. Moreover, there was a significant increase in both early and late apoptotic cell populations, correlating with the upregulation of pro-apoptotic genes (Bax and p53) and the downregulation of the anti-apoptotic gene Bcl-2. The chemical analysis identified 22 compounds in the unsaponifiable fraction, chiefly terpenoids such as phytol (65.74%). The saponifiable fraction presented a balanced composition of saturated (48.69%) and unsaturated (51.29%) fatty acids, with palmitic acid, linolenic acid, and linoleic acid as the predominant compounds.</p><p><strong>Conclusion: </strong>While the DCM fraction's relatively high IC₅₀ value may limit its utility as a standalone treatment, its ability to induce cell cycle arrest and apoptosis demonstrates its promise as a co-therapeutic agent with conventional anticancer drugs. Further research is essential to elucidate its precise mechanisms of action and to evaluate its efficacy in combination therapies, potentially advancing its role in cervical cancer treatment.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"189"},"PeriodicalIF":3.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiatherogenic and plaque stabilizing effects of saffron ethanolic extract in atherosclerotic rabbits.","authors":"Iman Nabilah Abd Rahim, Effat Omar, Suhaila Abd Muid, Noor Alicezah Mohd Kasim","doi":"10.1186/s12906-025-04927-6","DOIUrl":"10.1186/s12906-025-04927-6","url":null,"abstract":"<p><strong>Background: </strong>Saffron, the dried stigma of the flower Crocus sativus L., has been shown to have therapeutic effects on cardiovascular diseases. Several studies have explored the impact of saffron on atherosclerosis. However, the mechanism underlying the plaque-stabilizing and antiatherosclerotic effects of saffron has not been widely studied. Therefore, this study aimed to investigate the mechanism of the antiatherosclerotic and plaque-stabilizing effects of saffron ethanolic extract in experimentally induced atherosclerotic rabbits.</p><p><strong>Methods: </strong>New Zealand White rabbits were fed a 1% high-cholesterol diet (HCD) for 8 weeks to induce established atherosclerosis. The rabbits were then treated with 50 or 100 mg/kg/day saffron ethanolic extract (SAF), simvastatin (2.5 mg/kg/day) or placebo for another 8 weeks. Body weight, lipid profile, percentage of atherosclerotic lesions, immunohistochemical analysis, and quantitative real-time polymerase chain reaction were performed at baseline, after high-cholesterol diet feeding, and after the intervention.</p><p><strong>Results: </strong>The results showed that SAF had no significant effect on body weight. However, treatment with both doses of SAF markedly attenuated the levels of low-density lipoprotein (LDL) and total cholesterol (TC) in atherosclerotic rabbits. Higher doses of SAF markedly reduced atherosclerotic lesions in rabbit aortas. Additionally, SAF suppressed the tissue and gene expression of adhesion molecules and pro-inflammatory biomarkers in the aorta. SAF also reduced MMP-9 tissue expression in the aortas of atherosclerotic rabbits, thereby increasing plaque stability.</p><p><strong>Conclusions: </strong>Our findings suggest that saffron ethanolic extract exhibits therapeutic potential in rabbits with HCD-induced atherosclerosis. This effect may be associated with the modulation of inflammatory pathways, leading to reduced expression of pro-inflammatory cytokines, endothelial activation markers, and matrix metalloproteinases. The observed reduction in vascular inflammation and endothelial activation may contribute to improved lipid profiles, decreased atherosclerotic lesion severity, and enhanced plaque stability. While these findings highlight the potential of saffron ethanolic extract as an adjunctive treatment for atherosclerosis, further studies are warranted to clarify its direct effects on lipid metabolism and underlying molecular mechanisms.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"187"},"PeriodicalIF":3.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antioxidant effects and in vivo hepatoprotective effects of Osbeckia octandra, Vernonia cinerea and Atalantia ceylanica on a high fat diet induced metabolic dysfunction-associated steatotic liver disease mouse model.","authors":"Anuththara Upamali Abeykoon, Madhavi Priyanka Paranagama, Kavindra Kumara Wijesundera, Onela Canith Pathirana, Thilini Anupama Mahakapuge, Jayantha Rajapakse","doi":"10.1186/s12906-025-04918-7","DOIUrl":"10.1186/s12906-025-04918-7","url":null,"abstract":"<p><strong>Background: </strong>Metabolic dysfunction-associated steatotic liver disease (MASLD), which was formerly known as nonalcoholic fatty liver disease (NAFLD) has become a global epidemic that is predicted to affect more than 50% of the world population by 2040. However, except for Resmetiron, there are no approved drugs in conventional medicine to treat MASLD. The aim of this study was to explore the hepatoprotective effects of the aqueous extracts (AEs) of Osbeckia octandra (O. octandra), Vernonia cinerea (V. cinerea), and Atalantia ceylanica (A. ceylanica) which have been extensively used to treat hepatic diseases in the Sri Lankan traditional medicine.</p><p><strong>Methods: </strong>During initial in vitro experiments, aqueous extracts (AEs) of these three medicinal plants were prepared and evaluated for antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Trolox Equivalent Antioxidant Capacity (TEAC) assay. Cytotoxicity was assessed using the MTT assay on Vero cells to determine non-toxic doses for in vivo studies. For in vivo experiments, a high-fat diet (HFD)-induced MASLD mouse model was used. After co-treating the mice with AEs of the tested medicinal plants, their body weight gain was evaluated. Serum ALT, cholesterol, and triglyceride levels were analyzed using standard chemical methods. Hepatic lipid aggregation was evaluated by histopathology. Expression of TNF-α, IL-6, and PPAR-α genes was quantified using qRT-PCR. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test or LSD test. The level of significance was (p < 0.05).</p><p><strong>Results: </strong>Initial in vitro experiments revealed that AEs of these medicinal plants are rich sources of natural antioxidants with low cytotoxic effects. Subsequent in vivo experiments on the HFD-induced MASLD mouse model also demonstrated that AEs of these medicinal plants can ameliorate HFD-induced weight gain, dyslipidemia, and hepatic lipid aggregation. Reversal of downregulated PPAR-α gene expression confirmed their positive impact on lipid metabolism. Attenuation of increased serum ALT concentration provides evidence for their protective role against hepatic injury. In addition, these extracts could suppress the upregulation of TNF-α and IL-6 genes, which are crucial for inducing steatohepatitis.</p><p><strong>Conclusion: </strong>Aqueous extracts of O. octandra, V. cinerea, and A. ceylanica are rich in antioxidants and have the ability to suppress HFD-induced weight gain, dyslipidemias, hepatic steatosis, and hepatic inflammation providing solid evidence for their beneficial effects against initiation and progression of MASLD. Among these extracts, O. octandra stands out with the highest antioxidant activity, lowest cytotoxicity, and most promising hepatoprotective effects.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"186"},"PeriodicalIF":3.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the potential scabicidal activities of three essential oils: gaining mechanistic insights through in vitro contact bioassay and molecular docking.","authors":"Rofida Wahman, Shaymaa Mohamed, Soad Bayoumi, Rana Morsy, Salma Shafie, Nada Abdelraheem, Fatma Abdelaziz, Salma Hussein, Radwa Ibrahim, Norhan Mohammed, Doaa Yones, Sara Mohammed, Alzahraa Ahmad","doi":"10.1186/s12906-025-04868-0","DOIUrl":"10.1186/s12906-025-04868-0","url":null,"abstract":"<p><strong>Background: </strong>Scabies, sarcoptic itch, is a highly contagious and pruritic skin inflammation. Given their ecological benefits and previous therapeutic properties, essential oils are worth investigating as potentially safer alternatives to synthetic anti-scabies agents.</p><p><strong>Objectives: </strong>This study aimed to evaluate the effectiveness of three essential oils derived from cassia barks, myrrh oleo-gum-resin, and fennel fruits against Sarcoptes scabiei (Linnaeus, 1758), the causative agent of scabies, using lemongrass and clove essential oils as standards. Additionally, the study explored the structure-activity relationship by characterizing the chemical compositions of these essential oils. Further, a molecular docking study was performed to get further insights into the mechanism of the scabicidal effect of the active essential oils.</p><p><strong>Methods: </strong>Essential oils were extracted by hydro-distillation from dried cassia, myrrh, and fennel using the Clevenger apparatus, and their chemical profiles were characterized using gas chromatography-mass spectrometry (GC-MS) analysis. The contact bioassay method was utilized to assess their scabicidal activities. The molecular docking study incorporated two target enzymes, glutathione transferase (GST) and inactive serine proteases of scabies mite (SMIPP-S-D1). This is owing to the involvement of these two enzymes in the scabies defense mechanisms.</p><p><strong>Results: </strong>The GC-MS analysis identified (E)-cinnamaldehyde as the primary constituent in cassia essential oil, while β-ocimene, α-copaene, and trans-α-bisabolene were major components of myrrh essential oil. Fennel essential oil predominantly consisted of estragole. The contact bioassay demonstrated noteworthy scabicidal activities of cassia and myrrh essential oils, against all tested stages of S. scabiei. The docking analysis revealed higher binding affinities between the main phytochemicals of these active essential oils and GST, with binding scores ranging from - 7.7 to - 5.3 kcal/mol, compared to lemongrass and clove essential oils' main components. Additionally, these components displayed favorable binding affinities to SMIPP-S-D1 ranging from - 5.5 to - 4.0 kcal/mol, comparable to lemongrass and clove essential oils' main components. These findings suggest that cassia and myrrh essential oils could inhibit the defense mechanisms of scabies mites.</p><p><strong>Conclusion: </strong>These findings revealed the potential for anti-scabies of essential oils from cassia bark and myrrh oleo-gum-resin, which could effectively control scabies. The Docking analysis showed strong to moderate binding affinities of major components of cassia and myrrh essential oils towards GST and SMIPP-S-D1 enzymes.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"185"},"PeriodicalIF":3.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12100822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence-based evaluation for stroke guidelines mentioning traditional and complementary medicine rehabilitation.","authors":"Ran-Ran Zhu, Jin-Xiang Wang, Bei Pan, Hong-Hao Lai, Xiao-Ting Xu, Long Ge, Lei Fang","doi":"10.1186/s12906-025-04916-9","DOIUrl":"10.1186/s12906-025-04916-9","url":null,"abstract":"<p><strong>Background: </strong>Traditional and complementary medicine (T&CM) is often sought as an significant alternative intervention in stroke prevention and rehabilitation, and is recommended in several guidelines. However, little is known about the quality and consistency of T&CM rehabilitation recommendations for stroke in the guidelines.</p><p><strong>Methods: </strong>We systematically searched PubMed, Embase, CNKI, WOS, CBM, Duxiu, Wanfang, VIP, GIN, NICE, NGC, SIGN, BGS, NCCN, WHO guidelines, Google Scholar, MedSci, and Medilive from January 1990 to April 2024 for stroke rehabilitation guidelines that include T&CM recommendations. Our search strategy used search terms related to stroke, complementary and alternative therapy, and CPGs. Four independent reviewers used the Appraisal of Guidelines for Research and Evaluation II (AGREE II) instrument to evaluate the quality of the included guidelines. We summarized and analyzed the T&CM post-stroke rehabilitation recommendations.</p><p><strong>Results: </strong>Nineteen guidelines were included, of which nine were recommended for use according to the AGREE II scores. The highest scores were achieved in the domains of clarity of scope and purpose (90.50% ± 9.99%), while the lowest scores were achieved for applicability (45.56% ± 26.37%). Guidelines commonly recommended acupuncture to improve post-stroke spasticity and dysphagia. However, guidelines recommended against the addition of acupuncture to improve activities of daily living. Additionally, there was still controversy regarding the improvement of upper extremity motor function with acupuncture and specific recommendations for post-stroke cognitive impairment were scarce.</p><p><strong>Conclusions: </strong>The quality of stroke rehabilitation guidelines is acceptable, though improvements are needed in some domains, particularly applicability. The recommended guidelines show a high degree of agreement in recommending acupuncture to improve dysphagia after stroke, but recommendations in the areas of upper extremity motor dysfunction and cognitive impairment after stroke need to be further addressed. However, the strength of these recommendations is typically conditional and the level of evidence still needs to be further improved.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"184"},"PeriodicalIF":3.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Efficacy and safety of ayurvedic intervention (Sarpgandha Mishran) vs. amlodipine for stage‑i primary hypertension‑ study protocol for a prospective double‑dummy, double‑blind, placebo‑controlled randomized clinical trial.","authors":"Babita Yadav, Bcs Rao, Rajiv Narang, Sophia Jameela, Shruti Khanduri, Sakshi Sharma, Drishya Dinesh, Arti Srivastava, Richa Bhardwaj, Bharti Gupta, N Srikanth, Dharamvir Singh Arya, Richa Singhal","doi":"10.1186/s12906-025-04921-y","DOIUrl":"10.1186/s12906-025-04921-y","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"183"},"PeriodicalIF":3.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}