BMC Complementary Medicine and Therapies最新文献

{"title":"Assessment of in vitro skin permeation and accumulation of phenolic acids from honey and honey-based pharmaceutical formulations.","authors":"Anna Nowak, Anna Muzykiewicz-Szymańska, Magdalena Perużyńska, Edyta Kucharska, Łukasz Kucharski, Karolina Jakubczyk, Paulina Niedźwiedzka-Rystwej, Justyna Stefanowicz-Hajduk, Marek Droździk, Juraj Majtan","doi":"10.1186/s12906-025-04786-1","DOIUrl":"10.1186/s12906-025-04786-1","url":null,"abstract":"<p><strong>Background: </strong>Honey has been successfully used in wound care and cosmetics because of its effective biological properties, including antibacterial, antioxidant, and anti-inflammatory activities. Polyphenols, particularly phenolic acids, are key honey components responsible for these beneficial effects. In recent years, there has been a growing demand for natural, ecologically friendly, and biodegradable products in the modern cosmetics and wound care market. This study aimed to identify and quantify phenolic acids in four Polish honey samples of different botanical origins (heather, buckwheat, linden and rapeseed) and to assess for the first time the permeation of the identified phenolic acids through the skin and their accumulation after the application of pure honey samples, as well as honey-based hydrogel and emulsion formulations.</p><p><strong>Methods: </strong>The honey samples' antioxidant activity and total phenolic content were determined using the DPPH and ABTS assays and the Folin-Ciocalteu method, respectively. Phenolic acids and volatile compounds were identified and quantified in honey samples using the HPLC-UV and GC-MS method, respectively. The biocompatibility of the honey samples was evaluated using a murine fibroblast cell line (L929). A Franz-type vertical diffusion cell with porcine skin was used to assess phenolic acid's permeation and skin accumulation from different honey-based pharmaceutical formulations. The biodegradability of the prepared formulations was also characterised.</p><p><strong>Results: </strong>Gallic acid, 3,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, coumaric acid, and 3-hydroxybenzoic acid were identified and quantified in the honey samples. Heather honey exhibited significantly higher antioxidant activity and total polyphenol content than the other honey samples. Heather, linden and buckwheat honey samples significantly decreased cell viability at concentrations of 5% and 2.5%, while rapeseed honey sample markedly reduced fibroblast viability only at 5%. Among the tested formulations - pure honey, hydrogel, and emulsion - higher skin permeation and accumulation rates of phenolic acids were observed with the prepared honey-based hydrogels than with the pure honeys and emulsions. Additionally, the prepared formulations were classified as partially biodegradable.</p><p><strong>Conclusions: </strong>The obtained results confirmed the effectiveness of two pharmaceutical formulations in the form of a hydrogel or emulsion containing honey after applied topically. The inclusion of honey in the vehicle, in particular hydrogel increased the penetration of phenolic acids through the skin.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"43"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of Health Qigong and closed motor exercise on the physical and mental health of female drug rehabilitation participants: a randomized controlled trial.","authors":"Guang Yang, Deyu Meng, Shichun He, Meiqi Wei, Man Li, Lu Zhang, Zhendong Pan, Ziheng Wang","doi":"10.1186/s12906-025-04797-y","DOIUrl":"10.1186/s12906-025-04797-y","url":null,"abstract":"<p><strong>Background: </strong>Drug rehabilitation is a challenging process that impacts both the physical and mental health of individuals. Traditional martial arts, such as Health Qigong, and closed motor exercises, such as power cycling, have shown potential benefits in improving health outcomes. This study aims to compare the effects of Health Qigong, closed motor exercises, and their combination on the physical and mental health of female drug rehabilitation participants.</p><p><strong>Methods: </strong>In this randomized controlled trial, female participants from the Jilin Province Women's Compulsory Isolation Drug Rehabilitation Center were randomly assigned to three groups: Health Qigong (QigongG), Closed Motor Exercise (ClosedG), and Combined Health Qigong and Closed Motor Exercise (CombinedG). Measurements were taken at baseline, mid-intervention, and post-intervention and included resting heart rate, vital capacity, choice reaction time, sleep quality, and relapse tendency.</p><p><strong>Results: </strong>At the study's conclusion, the ClosedG group showed significant improvements in relapse tendency, vital capacity, and sleep quality compared to baseline. The QigongG showed significant improvements in relapse tendency, sleep quality, and choice reaction compared to baseline. The CombinedG group demonstrated significant improvements in relapse tendency, vital capacity, sleep quality, and choice reaction time, outperforming the ClosedG groups in reaction time, and outperforming QigongG groups in vital capacity. The CombinedG group exhibited the most notable overall improvements.</p><p><strong>Conclusion: </strong>The combined intervention of Health Qigong and closed motor exercises is more effective in improving physical and mental health metrics among female drug rehabilitation participants than either intervention alone. These findings suggest that incorporating a combination of traditional martial arts and closed motor exercises could enhance rehabilitation programs for drug rehabilitation.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov NCT06454565. The date of registration is 2024.07.11 (Retrospectively registered).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"38"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tangeretin offers neuroprotection against colchicine-induced memory impairment in Wistar rats by modulating the antioxidant milieu, inflammatory mediators and oxidative stress in the brain tissue.","authors":"Olalekan Bukunmi Ogunro, Mojisola Esther Karigidi, Gideon Ampoma Gyebi, Areej Turkistani, Ahmad H Almehmadi","doi":"10.1186/s12906-025-04769-2","DOIUrl":"10.1186/s12906-025-04769-2","url":null,"abstract":"<p><strong>Background: </strong>Tangeretin, a flavone compound (O-polymethoxylated) naturally present in tangerine and other citrus peels has demonstrated effectiveness as an anti-inflammatory and neuroprotective agent in several disease model. This study evaluated the impact of tangeretin in mitigating cognitive dysfunction and oxidative stress induced by colchicine in rats, comparing its efficacy with donepezil hydrochloride.</p><p><strong>Methods: </strong>Cognitive dysfunction was induced by administering colchicine (15 µg/rat) intracerebroventricularly (ICV) via a stereotaxic apparatus in male Wistar rats. Colchicine resulted in poor memory retention in acquiring and retaining a spatial navigation task, passive avoidance apparatus, and Morris water maze paradigms. Chronic treatment with tangeretin (at doses of 50, 100, and 200 mg/kg, p.o. once daily) and donepezil hydrochloride (at a dose of 10 mg/kg, p.o. daily) for 28 days, starting seven days before colchicine injection, significantly ameliorated colchicine-induced cognitive impairment.</p><p><strong>Results: </strong>The biochemical analysis showed that chronic administration of tangeretin effectively reversed the colchicine-induced increase in the level/activity of lipid peroxidation, hydrogen peroxide (H₂O₂), myeloperoxidase (MPO), nitrite, reactive oxygen species (ROS), tumour necrosis factor-α (TNF-α), nuclear factor kappa B (NF-κB), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), serotonin, dopamine, glutamate, amyloid beta (Aβ) peptide, and caspase-3. Tangeretin also reversed the colchicine-induced reduction in the level/activity of brain-derived neurotrophic factor (BDNF), amma-aminobutyric acid (GABA), acetylcholinesterase (AChE), glutathione S-Transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and total thiol (T-SH) in rat brains. However, donepezil hydrochloride did not prevent oxidative stress.</p><p><strong>Conclusions: </strong>These findings suggest that chronic administration of tangeretin at 50, 100, and 200 mg/kg, p.o. once daily, was protective in mitigating colchicine-induced cognitive impairment and associated oxidative stress. At the same time, donepezil hydrochloride did not demonstrate similar effects.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"40"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the cervical and thoracic \"Daoyin\" training on posture and pulmonary function in patients with upper crossed syndrome: a randomized controlled trial.","authors":"Yue Guo, Ming Li, Chaowei Xie, Xiaoyan Liu, Yushan Chen, Jun Yang, Yuxue Wu, Shaoqing Chen, Shizhong Wang, Jianping Lin","doi":"10.1186/s12906-025-04791-4","DOIUrl":"10.1186/s12906-025-04791-4","url":null,"abstract":"<p><strong>Objectives: </strong>With the growing ubiquity of electronic devices, the incidence of upper crossed syndrome (UCS) is increasing every year and tends to affect younger populations. The study was designed to assess the effect of cervical and thoracic \"Daoyin\" training on pain, posture, pulmonary function, cervical range of motion, and emotional state in college students with UCS.</p><p><strong>Design: </strong>We conducted a parallel-group randomized control study.</p><p><strong>Setting: </strong>The Fujian Medical University in China.</p><p><strong>Participants: </strong>74 patients with UCS fulfilled the study requirement and were assigned to the intervention group (IG) and the control group (CG).</p><p><strong>Intervention: </strong>Instructed cervical and thoracic \"Daoyin\" training was given to students in the IG for eight weeks, five days a week for 45 min, whereas students in the CG continued with their usual activities.</p><p><strong>Main outcome measures: </strong>Primary outcomes, including forward head and shoulder angles, pain and cervical spine function, as well as secondary outcomes, such as pulmonary function, cervical range of motion and emotional state, were measured at baseline and a follow-up of 8-week. These measurements were conducted using photogrammetry, visual analog scale (VAS), Neck Disability Index (NDI), pulmonary function, cervical range of motion test and Self-rating Anxiety Scale (SAS), respectively. The statistical analysis used the change value between the baseline and the 8-week intervention measurements.</p><p><strong>Results: </strong>74 patients (IG = 37; CG = 37) were randomized. All participants were included in the analysis. There were significant differences of the change values between groups in Forward Head Angle (FHA) (d = 1.113, P < 0.001), Forward Shoulder Angle (FSA) (d = 1.052, P < 0.001), VAS (d = 0.854, P < 0.001), NDI (d = 0.754, P = 0.005), pulmonary function (Forced Expiratory Volume in 1 s/Forced Vital Capacity (FEV1/FVC) (d = 0.516, P = 0.002), maximal voluntary ventilation (MVV) (d = 0.504, P = 0.033), cervical range of motion in Left Rotation (LR) (d = 0.647, P = 0.014) and emotional state (d = 0.587, P = 0.014). No side effects were observed in this study.</p><p><strong>Conclusion: </strong>Cervical and thoracic \"Daoyin\" training can improve the abnormal posture of head forward and round shoulders, reducing pain and anxiety, improving cervical spine function, and improving lung function to a certain extent.</p><p><strong>Trial registration: </strong>ChiCTR2300072669 (20/06/2023), Prospective registration, www.chictr.org.cn .</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"41"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antibacterial and anti-biofilm activity of two Iranian medical-grade kinds of honey on multidrug-resistant Pseudomonas aeruginosa.","authors":"Mustafa Riyadh Alshaybawee, Shahrzad Asgari, Paria Ghadersoltani, Ahmadreza Mehrabian, Parastoo Saniee","doi":"10.1186/s12906-025-04778-1","DOIUrl":"10.1186/s12906-025-04778-1","url":null,"abstract":"<p><strong>Introduction: </strong>Pseudomonas aeruginosa is a prominent multidrug-resistant and biofilm-forming bacteria. Mono-floral honey, enriched with a variety of biological compounds, can be categorized as medical-grade honey due to its notable pharmacological benefits. In this study, two types of Iranian honey were thoroughly characterized, and the antimicrobial and anti-biofilm properties were examined against three clinical strains of multidrug-resistant P. aeruginosa.</p><p><strong>Methods: </strong>Citrus and Thyme honey from Alborz were selected based on physicochemical, phytochemical, and melissopalynological tests conducted from a medical perspective. The antibacterial activity of the honey samples against three clinical strains of multidrug-resistant P. aeruginosa isolated from wound infections was evaluated using both the well-diffusion and broth microdilution methods. Additionally, an antibiofilm assay was performed using the crystal violet method in microplates.</p><p><strong>Results: </strong>Both medical grade honey samples exhibited considerable antibacterial activity against the three P. aeruginosa isolates at 75-100% v/v concentrations with inhibition zones measuring between 15 and 30 mm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values for both types of honey were 6.25% v/v (final concentration). The antibiofilm assay indicated that both types of honey demonstrated varying levels of antibiofilm activity. Citrus honey at 9% concentration was the most effective, showing an average inhibition rate of 59%, while Citrus honey at 2.3% final concentration exhibited the least effectiveness with an average inhibition rate of 23%.</p><p><strong>Discussion: </strong>A thorough analysis of the honeys studied confirmed their authenticity and the presence of medicinal compounds. The results of honey tests correspond to the normal range (natural Honey) in the Council of the European Union. Based on the evaluation and compliance with the medical grade criteria including authenticity, health, qualities well botanical origin mentioned honey is classified in medical grade. The antibacterial results indicated that both Thyme and Citrus honeys effectively inhibit the growth and biofilm formation of P. aeruginosa. Therefore, these honeys may serve as natural and safe alternatives or adjuncts to conventional antibiotic therapy for wound healing and infection management.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"39"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balneotherapy for the treatment of post-COVID syndrome: a randomized controlled trial.","authors":"Diana Ovejero, Anna Ribes, Judit Villar-García, Marta Trenchs-Rodriguez, Daniel Lopez, Xavier Nogués, Robert Güerri-Fernandez, Natalia Garcia-Giralt","doi":"10.1186/s12906-025-04784-3","DOIUrl":"10.1186/s12906-025-04784-3","url":null,"abstract":"<p><strong>Background: </strong>Post-Acute COVID Syndrome (PACS) is a complex disorder that currently lacks effective evidenced-based therapies to manage it. This randomized controlled trial aims to evaluate the effects of balneotherapy (BT) on PACS symptomatology.</p><p><strong>Methods: </strong>Ninety-eight adults with PACS visited at Hospital del Mar Research Institute, Barcelona (Spain) were included to the study. Participants in the intervention group (n = 51) were allocated to 12 sessions of BT and aquatic exercises delivered in one month while the control group (n = 47) did not. The primary outcome was to evaluate the absolute change in questionnaire scores between baseline and two follow-up points: immediately after balneotherapy (or one-month post-baseline for the control group) and 2 months post-baseline. The following scales/questionnaires were employed: Post-COVID-19 functional status scale, mMRC dyspnea Scale, SF-36, Pittsburgh Sleep Quality Index (PSQI), Hospital Anxiety and Depression Scale (HADS), Memory failures in everyday life following severe head injury, and Visual Analogic Scale (VAS).</p><p><strong>Results: </strong>Forty-seven patients in the BT group and 43 in the control group completed the study. The majority of participants were middle-aged women (> 84%; mean age 48 years), and the most prevalent symptoms were fatigue, musculoskeletal pain, and neurocognitive impairment (> 88%). Noteworthy, the vast majority did not undergo a severe primary infection (ICU admissions < 3%). After BT, significant improvement was detected in the BT group vs. the control group in various SF-36 domains, PSQI total score (Beta-coefficient [95%CI] 2.641 [1.15;4.12]; p -value = 0.003), HAD's anxiety subscale (Beta-coefficient [95%CI] 1.72 [0.40;3.03;p-value = 0.023), and VAS (Beta-coefficient [95%CI] 1.625 [0.32;2.96]; p-value = 0.026). Among these, SF-36's energy/fatigue and pain subscales exhibited the most prominent changes with a Beta-coefficient [95%CI] of -17.45 [-24.23;-10.66] and - 21.634 [-30.48;-12.78], respectively (p-value < 0.0001). No severe adverse effects were reported during BT although seventeen patients reported mild and transient worsening of preexisting symptoms, particularly fatigue/post-exertional malaise mainly in the first sessions of BT.</p><p><strong>Conclusion: </strong>Balneotherapy comprise an effective therapeutic modality that can alleviate several symptoms that characterize PACS, particularly musculoskeletal pain and fatigue. However, the sustainability of these effects over time remains uncertain, as evidenced by the loss of some between-group differences at the one-month follow-up.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov NCT05765591 (13/03/2023).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"37"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anticancer potential of extracts and compounds from the heartwood of Cotinus coggygria Scop. wild growing in Serbia.","authors":"Ivana Pašić, Miroslav Novaković, Vele Tešević, Slobodan Milosavljević, Nina Petrović, Tatjana Stanojković, Ivana Z Matić","doi":"10.1186/s12906-025-04768-3","DOIUrl":"10.1186/s12906-025-04768-3","url":null,"abstract":"<p><strong>Background: </strong>Cotinus coggygria has a long history of use in traditional medicine in Europe and Asia. The aim of study was to explore the cytotoxicity of extracts (EE-ethanol, MME-methylene chloride/methanol, and WE-water) and compounds (butin, butein, fisetin, sulfuretin, taxifolin, eriodictyol, fustin, cotinignan A, sulfuretin auronol, 3-O-methylepifustin, 3-O-methylfustin, and sitosterol-3-O-β-D-glucoside) isolated from C. coggygria. Mechanisms of anticancer effects of three extracts, butin, butein, and sulfuretin were examined.</p><p><strong>Methods: </strong>Compounds were isolated from the EE using silica gel column chromatography and semipreparative HPLC. Structure elucidation was performed using NMR spectroscopy. Cytotoxicity was evaluated using MTT assay. The effects on cell cycle and cell death were investigated by flow cytometry. The antimigration effects were examined by scratch assay, while expression of the MMP2, MMP9, and VEGFA were measured by quantitative real time PCR. The antioxidant effects were examined by flow cytometry.</p><p><strong>Results: </strong>3-O-methylepifustin, epitaxifolin, and sulfuretin auronol were found for the first time in C. coggygria. The extracts and compounds showed selective cytotoxicity against HeLa, MDA-MB-231, HL-60, K562, A375, PC-3, and DU 145 cells. HeLa cells were the most sensitive to the cytotoxicity of MME (IC₅₀ value of 47.45 µg/mL), while leukemia K562 and HL-60 cells were the most sensitive to the MME and EE (IC₅₀ values in the range from 31.04 to 44.57 µg/mL). Butein exerted strong cytotoxicity on HeLa, K562, and MDA-MB-231 cells (IC₅₀ values of 8.66 µM, 13.91 µM, and 22.36 µM). EE, butin, butein, sulfuretin, and fisetin were highly selective against leukemia K562 cells when compared with normal fibroblasts MRC-5 (selectivity index: 4.01, 5.15, 6.17, 7.05, > 4.41, respectively). Butein and fisetin showed high selectivity in the cytotoxic activity against HeLa cells when compared with MRC-5 cells (selectivity index: 9.91 and > 6.61). Three extracts, butin, butein, and sulfuretin, initiated apoptosis in HeLa cells by activating caspase-8 and caspase-9. The extracts, butin, butein, and sulfuretin inhibited HeLa cell migration. EE, MME, butein, and sulfuretin exerted cytoprotective effects in normal fibroblasts.</p><p><strong>Conclusions: </strong>This research might suggest promising anticancer effects and underscores the need for additional research on C. coggygria extracts and compounds to assess their potential in cancer prevention and therapy.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"36"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomised control trial to compare the efficacy of traditional Thai massage and ultrasound therapy for treating plantar heel pain.","authors":"Supamas Somphai, Wiraphong Sucharit, Punnee Peungsuwan, Neil Roberts, Uraiwan Chatchawan","doi":"10.1186/s12906-025-04754-9","DOIUrl":"10.1186/s12906-025-04754-9","url":null,"abstract":"<p><strong>Background: </strong>Massage is suggested to be an effective treatment for chronic plantar heel pain (PHP). There is, however, no scientific evidence to support this claim. In the present study Traditional Thai Massage (TTM) has been compared with Ultrasound therapy (US) for treating PHP.</p><p><strong>Methods: </strong>Sixty PHP patients with a Myofascial Trigger Point (MTrP) present in the calf were randomly assigned to receive a 40-minute single treatment of either US or TTM. Pain Intensity (VAS), Pressure Pain Threshold (PPT), Ankle Dorsiflexion Range of Motion (DROM), and Foot Skin Temperature (FST), were measured before, immediately after, and 24 h after treatment.</p><p><strong>Results: </strong>Compared to baseline, both groups showed a significant reduction in pain intensity immediately (CVAS) and 24 h after treatment (MVAS24) (p < 0.01), as well as a significant increase in PPT of the heel immediately after treatment (p < 0.05). However, only the US treatment group showed an increase in PPT in the calf immediately after treatment (p < 0.05). Furthermore, only the US group showed a significant increase in DROM immediately and 24 h after treatment (p < 0.001). The reduction in CVAS and increase in DROM immediately (p < 0.05) and 24 h after treatment (p < 0.01) were significantly greater in the US than the TTM group.</p><p><strong>Conclusions: </strong>The significant efficacy of US with stretching for providing pain relief in the treatment of PHP is confirmed. For the first time, TTM has also been demonstrated to be effective in providing pain relief for patients with PHP and may have a potentially useful complementary role, in treating PHP.</p><p><strong>Trial registration: </strong>TCTR20210909001 (First Submitted Date: September 2021).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"42"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of total phenolic and flavonoid contents, antioxidant and antibacterial potential of the bark extracts of Syzygium guineense (Wild.) DC.","authors":"Engeda Dessalegn, Mengisteab Mathewos, Hiwot Gebremeskel, Nigatu Tuasha","doi":"10.1186/s12906-025-04788-z","DOIUrl":"10.1186/s12906-025-04788-z","url":null,"abstract":"<p><strong>Background: </strong>Syzygium guineense (Wild.) DC. is a wild indigenous tree widely used as a traditional medicine for various human ailments in Ethiopia. The purpose of this study was to quantify total phenolic (TPC) and total flavonoid (TFC) contents and determine the antioxidant and antibacterial activities of various solvent extracts of the bark of the plant.</p><p><strong>Methods: </strong>The TPC and TFC were determined using Folin-Ciocalteu and aluminum chloride methods, respectively. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, ferric-reducing power, and total antioxidant capacity assays were used to evaluate the antioxidant activities. Antibacterial properties were determined using the disc-diffusion and broth dilution assays.</p><p><strong>Results: </strong>The ethanol extract of the bark was found to have high TPC (37.80 ± 3.70 mgGAE/g) and TFC (19.22 ± 1.44 mgQE/g). Similarly, the ethanol extract showed stronger DPPH scavenging activity (EC₅₀ = 5.62 μg/mL). The ferric-reducing power and total antioxidant capacity were also strong (163.08±11.67 mgAAE/g and 143.72±2.86 mgBHTE/g of dried extract of 1 mg/mL, respectively). The lowest MIC was observed in acetone extract against S. aureus (1.56 mg/mL) and in ethanol extract against K. pneumoniae (1.56 mg/mL).</p><p><strong>Conclusion: </strong>The ethanol extract of the bark of S. guineense possesses high TPC and TFC. In addition, it showed strong ferric-reducing power and total antioxidant capacity, asserting high antioxidant content. The extracts have shown antibacterial activities against both Gram-positive (S. aureus) and Gram-negative bacterial species. Thus, further in-depth investigations may warrant the isolation of powerful antioxidants and potent antimicrobial agents from the plant.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"35"},"PeriodicalIF":3.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells.","authors":"Sukannika Tubtimsri, Tiraniti Chuenbarn, Suwisit Manmuan","doi":"10.1186/s12906-025-04782-5","DOIUrl":"10.1186/s12906-025-04782-5","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Plant flavonoids such as quercetin are useful for both the therapeutic and preventive care of a variety of illnesses. Nevertheless, their antitumor efficacy against KON oral cancer is still unknown. Therefore, the aim of this investigation was to examine quercetin's anti-growth, anti-migrative, and anti-invasive characteristics. The cell cycle arrest property and mitochondrial function disruption of quercetin were also investigated. Additionally, the cellular mechanism responsible for inducing apoptosis and the anti-metastasis mechanism were identified.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;KON cells were treated with quercetin in order to test the anticancer activity of this compound. The MTT colorimetric assay was used to examine the cell viability of the treated cells in comparison to MRC-5 fibroblast cells. After being exposed to the detrimental effects of quercetin, the morphology of the KON cells was examined using DAPI and FDA double staining, as well as Hoechst 33,258 and AO double staining. Annexin V-FITC with a flow cytometer and DCFDA labeling were used to detect apoptosis induction and the ROS production associated with cell death. Quercetin's ability to stop the cell cycle was evaluated via PI staining and the flow cytometer. The examination included anti-proliferative, anti-migration, and anti-invasion activities. Values for the transepithelial electrical resistance, or TEER, were measured. Ultimately, the mechanisms of action of the apoptotic markers and genes implicated in the metastatic process were clarified.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Quercetin treatment reduced the vitality of KON cells and had minimal effect on MRC cells. Following quercetin treatment, the characterization of apoptosis and cell death in KON cells was observed. When quercetin was applied to KON cells, the generation of ROS increased. Furthermore, it was discovered that quercetin increased the percentage of dead cells and cell cycle arrests in the S and G2/M phases. Moreover, quercetin inhibited KON cells' capacity for migration and invasion in addition to their effects on cell stability and structure. As a result of identifying the mechanism responsible for inducing apoptosis and preventing metastasis, quercetin was found to downregulate the expression of BCL-2/BCL-XL while increasing the expression of BAX. TIMP-1 expression was upregulated while MMP-2 and MMP-9 were downregulated. Quercetin's anticancer properties and specific mechanisms of action in relation to KON cells were clarified.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Quercetin is greatly cytotoxic in oral cancer cells, triggering cells undergoing apoptosis and ROS-mediated cell death, possessing S and G2/M cell cycle arrest properties, and exhibiting anti-metastatic activities. Finally, this discovery opens up a wide range of possibilities for developing an anti-oral cancer drug and further investigating its effectiveness in vivo and in clinical trials as an alternative can","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"34"},"PeriodicalIF":3.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}