Exploring antioxidant properties and antidiabetic potential of ten underutilized Sri Lankan fruits.

IF 3.4 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
P G N H Dharmasiri, P Ranasinghe, R G P T Jayasooriya, K W Samarakoon
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引用次数: 0

Abstract

Background: Managing and treating diabetes mellitus is one of the scientific community's biggest challenges. This study aimed to determine phytochemicals, antioxidants, and antidiabetic properties of different solvent extracts of fruit plants as a source of natural products.

Methods: The total flavonoid content (TFC) and total phenolic content (TPC) were determined using standard methods. Antioxidant activities were determined using in vitro ferric reducing power (FRAP), oxygen radical absorbance capacity (ORAC), DPPH radical scavenging, and ABTS radical scavenging assay methods. The antidiabetic activities of the fruit extract were assessed using in vitro anti-amylase,anti-glucosidase , and anti-glycation assays.

Results: Among different fractions of crude (CR), hexane (HX), ethyl acetate (EA), and aqueous (AQ), the highest TPC and TFC values were found in the HX fraction and EA fraction of Garcinia xanthochymus, respectively. The highest FRAP, ORAC, DPPH, and ABTS activities were found in the CR extract of G. xanthochymus, EA of G. xanthochymus, EA of Flacourtia indica, and HX fraction of G. xanthochymus, respectively. The highest anti-amylase inhibition was found in EA of G. xanthochymus, EA fraction of Careya arborea, and HX fraction of Syzygium zeylanicum compared to the standard drug Acarbose. The highest anti-glucosidase enzyme activities were found in the EA fraction of Elaeocarpus angustifolia, followed by the EA fraction of Cynometra cauliflora, the EA fraction of S. zeylanicum, the CR extract of F. indica, the AQ fraction of C. cauliflora, the EA fraction of C. arborea, the EA fraction of G. xanthochymus, the CR extract of C. cauliflora, and the CR extract of E. angustifolia compared to Acarbose. Antiglycation activities were higher in the HX fraction of S. zeylanicum, the EA fraction of G. xanthochymus, and C. arborea than in Rutin. None of the extracts showed inhibition for human salivary α-amylase enzyme.

Conclusion: The ethyl acetate (EA) fractions of G. xanthochymus and C. arborea, along with the hexane (HX) fraction of S. zeylanicum, exhibited strong in vitro antioxidant and antidiabetic activities. These results indicate their potential as promising therapeutic candidates for the management of diabetes mellitus. However, further in vivo investigations are warranted to elucidate their mechanisms of action and assess their clinical applicability.

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探索十种未充分利用的斯里兰卡水果的抗氧化特性和抗糖尿病潜力。
背景:管理和治疗糖尿病是科学界面临的最大挑战之一。本研究旨在确定作为天然产物来源的不同溶剂提取物的植物化学物质、抗氧化剂和抗糖尿病特性。方法:采用标准方法测定黄酮类含量(TFC)和总酚含量(TPC)。采用体外铁还原能力(FRAP)、氧自由基吸收能力(ORAC)、DPPH自由基清除能力和ABTS自由基清除能力测定抗氧化活性。通过体外抗淀粉酶、抗葡萄糖苷酶和抗糖基化试验,评估了果实提取物的抗糖尿病活性。结果:在粗馏分(CR)、正己烷馏分(HX)、乙酸乙酯馏分(EA)和水馏分(AQ)中,黄藤黄的TPC和TFC值分别以HX馏分和EA馏分最高。其中,黄荷叶CR提取物、黄荷叶EA、黄荷叶EA和黄荷叶HX部位的FRAP、ORAC、DPPH和ABTS活性最高。与标准药物阿卡波糖相比,黄松EA、杉木EA部位和泽兰HX部位的抗淀粉酶抑制作用最强。抗葡萄糖苷酶活性最高的部位为油松果的EA部位,其次为花椰菜的EA部位、玉米叶的EA部位、鸢尾果的CR提取物、花椰菜的AQ部位、油松果的EA部位、黄杉果的EA部位、油松果的CR提取物、油松果的CR提取物以及与阿卡波糖相比的油松果的CR提取物。白花参的HX部位、黄花参的EA部位和木参的抗糖化活性均高于芦丁。对人唾液α-淀粉酶均无抑制作用。结论:黄松、木松的乙酸乙酯(EA)部位和泽兰的己烷(HX)部位均具有较强的体外抗氧化和抗糖尿病活性。这些结果表明它们有潜力成为治疗糖尿病的有希望的候选药物。然而,需要进一步的体内研究来阐明其作用机制并评估其临床适用性。
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来源期刊
BMC Complementary Medicine and Therapies
BMC Complementary Medicine and Therapies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
6.10
自引率
2.60%
发文量
300
审稿时长
19 weeks
期刊介绍:
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