BMC Complementary Medicine and Therapies最新文献

{"title":"Manuka honey as a multifunctional therapeutic against Helicobacter pylori: from chemical profiling to in vitro potency and in vivo gastroprotective efficacy.","authors":"Wafaa Maher Abd Elaziz, Eman E Abd Elhady, Mai Hussein Abd-Elfatah, Mohammed Yosri","doi":"10.1186/s12906-026-05491-3","DOIUrl":"10.1186/s12906-026-05491-3","url":null,"abstract":"<p><p>Manuka honey contains bioactive phenolic and flavonoid chemicals with antibacterial and anti-inflammatory effects. The chemical profile and therapeutic efficiency of Manuka honey against Helicobacter pylori infection were investigated utilizing in-vitro and in-vivo models. The phenolic and flavonoid contents were profiled using HPLC. The antibacterial property of Manuka honey extract was determined using inhibitory zones, MIC, MBC, and time-kill tests. An H. pylori rat model was utilized to investigate changes in weight, bacterial burden, histological modifications, NF-κB expression, inflammatory cytokines (TNF-α, IL-4), oxidative stress indicators (CAT, SOD, MDA, NO), and biochemical parameters such as kidney and liver functioning. HPLC examination identified seven phenolic chemicals, the most prevalent of which were coumaric, cinnamic, and chlorogenic acids, as well as seven flavonoids, dominated by chrysin, rutin, and kaempferol. Manuka honey demonstrated strong anti-H. pylori activity (inhibition zone 15.1 mm), equivalent to conventional medications with MIC 31.25 µg/mL and MBC 62.5 µg/mL. Time-kill curves revealed no significant differences from normal therapy. Manuka honey treated infected rats by restoring body weight, reducing stomach bacterial load, improving histology, and downregulating NF-κB expression. The treated rats showed decreased TNF-α, increased IL-4, improved antioxidant enzyme levels, lower oxidative stress indicators, and normalized liver and kidney functioning. Manuka honey has substantial anti-H. pylori capacity, substantial anti-inflammatory properties, oxidative stress regulation, and protection of gastric tissue, hepatic, and renal functions, indicating its therapeutic usefulness as a natural complementary to H. pylori infection.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13445924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and antidiabetic evaluation of Bauhinia tomentosa L. aerial parts.","authors":"Lourin G Malak, Mai A M Ahmed, Ereny M Abdelmalek, Rofida Wahman, Mariam A Nicola, Adel A Gomaa","doi":"10.1186/s12906-026-05484-2","DOIUrl":"10.1186/s12906-026-05484-2","url":null,"abstract":"<p><strong>Background: </strong>Members of the genus Bauhinia L., commonly known as cow's hoof, are important species in the family Fabaceae and are traditionally used to treat diabetes and other diseases. This study aimed to identify antidiabetic bioactive compounds from Bauhinia tomentosa aerial parts via bioassay-directed fractionation.</p><p><strong>Methods: </strong>Determination of serum glucose and insulin levels was conducted on nicotinamide + STZ-induced diabetic rats treated with different fractions via a glucose oxidase assay kit and a rat insulin ELISA kit. In addition, GLUT4 protein levels in muscle homogenates were quantified via an ELISA kit. The ethyl acetate (EtOAc) fraction (the most active fraction) was fractionated via column chromatography (CC), and the isolated compounds were identified on the basis of their spectroscopic data. Additionally, the EtOAc fraction was characterized via LC‒QTOF‒MS/MS analysis. All the isolated compounds were evaluated for their antioxidant, α-glucosidase, and α-amylase inhibitory activities.</p><p><strong>Results: </strong>The EtOAc fraction markedly reduced the serum glucose level in the nicotinamide + STZ-induced type 2 diabetic rats (136.6 ± 12.17 mg/dL). Moreover, it showed a significant increase in serum insulin level (0.344 ± 0.04 µIU/mL). Four compounds (1-4) were isolated, of which luteolin showed potent inhibition of α-amylase activity, with an IC50 value of 19.32 ± 1.046 µM, as well as significant DPPH scavenging capacity. The LC-QTOF-MS/MS profiling validated the phytoconstituent composition and confirmed its chemotaxonomic relationship with closely related species.</p><p><strong>Conclusions: </strong>The antidiabetic effects of EtOAc fraction and its isolates may be attributed to its insulinotropic, antioxidant, and carbohydrate-hydrolysing enzyme inhibitory activities. Luteolin has been shown to be at least partially responsible for the antidiabetic activity of B. tomentosa.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13430811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antibacterial activity of selected medicinal plant extracts against Escherichia coli in Adami Tulu Jiddo Kombolcha district, Ethiopia.","authors":"Ashenafi Gizaw Habte, Hailemariam Mihiretie Goshu, Megersa Idris Ahmed, Kedir Sali Musa, Gelan Dule Dahesa","doi":"10.1186/s12906-026-05489-x","DOIUrl":"https://doi.org/10.1186/s12906-026-05489-x","url":null,"abstract":"<p><strong>Background: </strong>The increasing prevalence of antimicrobial-resistant Escherichia coli necessitates the exploration of alternative antimicrobial agents from botanical sources.</p><p><strong>Objective: </strong>This study evaluated the in vitro antibacterial activity of methanolic and ethanolic leaf extracts of Datura stramonium, Balanites aegyptiaca, and Acokanthera schimperi against field and reference strains of E. coli.</p><p><strong>Methods: </strong>Following phytochemical screening, antibacterial activity was determined using the disk diffusion method, while Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined using the broth dilution method.</p><p><strong>Results: </strong>All extracts demonstrated concentration-dependent inhibitory effects. The methanolic extract of D. stramonium (100 mg/mL) exhibited the highest antibacterial activity, producing a zone of inhibition of 20.27 ± 0.37 mm against the field isolate. This inhibition zone was similar to that of the ciprofloxacin positive control (19.7 ± 0.3 mm). Methanol consistently demonstrated higher extraction yield and antibacterial activity than ethanol across most tested species. D. stramonium and A. schimperi showed MIC values as low as 0.78 mg/mL, while all extracts exhibited bactericidal activity based on an MBC/MIC ratio of 2.</p><p><strong>Conclusion: </strong>These findings support the potential of the selected medicinal plants as sources of antibacterial compounds and provide a basis for further investigations involving bioactive compound characterization, safety assessment, and in vivo validation.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of endophytic bacteria from Echinacea purpurea and Calendula officinalis with antimicrobial and anticancer activities.","authors":"Razieh Mohseni, Hasan Shojaei, Mohammad Sadegh Damavandi","doi":"10.1186/s12906-026-05464-6","DOIUrl":"https://doi.org/10.1186/s12906-026-05464-6","url":null,"abstract":"<p><strong>Background: </strong>Endophytic bacteria are increasingly recognized as a promising source of biologically active secondary metabolites with potential biomedical applications. This study aimed to evaluate the antimicrobial and anticancer activities of secondary metabolite extracts derived from endophytic bacteria associated with Echinacea purpura (E. purpurea) and Calendula officinalis (C. officinalis).</p><p><strong>Methods: </strong>Bacterial endophytes were identified using phenotypic characterization and 16 S rRNA gene sequencing. Secondary metabolites were extracted using ethyl acetate and evaluated for antimicrobial activity using agar well diffusion and broth microdilution assays, including determination of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC). Cytotoxic and apoptosis-inducing effects were assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry assays. Chemical profiling was performed using Fourier-transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS).</p><p><strong>Results: </strong>Three isolates (SM.Ep01, SM.Co01, and SM.Co05) demonstrated measurable antimicrobial activity, predominantly against Gram-positive bacteria. SEM analysis revealed structural alterations in bacterial cells following treatment. Crude metabolite extracts induced dose-dependent cytotoxicity and apoptosis in cancer cell lines. FTIR and GC-MS analyses revealed diverse functional groups and tentatively identified compounds previously associated with biological activity.</p><p><strong>Conclusion: </strong>These findings provide preliminary evidence that endophytic bacteria associated with E. purpurea and C. officinalis may represent potential sources of biologically active metabolites.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13654212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149165268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing lumbar therapy: a biomechanical study of oblique pulling manipulation under different postures.","authors":"Yanfei Wang, Aorui Yu, Lianhai Jin, Weidong Liu, Dayu Chen, Yafei Cao, Kun Gao","doi":"10.1186/s12906-026-05477-1","DOIUrl":"10.1186/s12906-026-05477-1","url":null,"abstract":"<p><strong>Background: </strong>While oblique pulling manipulation has proven to be an effective conservative treatment approach for lumbar disc herniation, it is not without limitations. One of the primary drawbacks of this technique is the potential for inaccurate localization during the procedure. This study aims to explore the effects of the oblique pulling manipulation under different lumbar postures and to characterize the resulting load distribution across different lumbar regions.</p><p><strong>Methods/study design: </strong>This study employed a multi-modal approach combining infrared optical motion capture, finite element modeling, and biomechanical experiments. Eighteen healthy volunteers were divided into three groups for motion capture analysis of the oblique pulling manipulation at different angles. A finite element model of the lumbar spine was developed based on CT scans of a 24-year-old male volunteer and validated against existing literature. Biomechanical experiments were conducted on six cadaveric specimens using a BOSE testing machine to simulate the manipulation technique. Pressure measurements in the intervertebral discs and facet joints were obtained using a Tekscan pressure sensor.</p><p><strong>Results: </strong>The experimental results showed that the biomechanical effects of oblique pulling manipulation were closely related to lumbar posture. Infrared optical motion capture, finite element modeling, and biomechanical experiments consistently demonstrated posture-dependent, region-specific loading patterns, with peak forces preferentially concentrated around the L3/4 region in extension, the L4/5 region in flexion, and the L5/S1 region in excessive flexion. These findings suggest that adjusting lumbar posture may influence the regional distribution of mechanical loads and provide a biomechanical basis for posture-guided application of oblique pulling manipulation.</p><p><strong>Conclusion: </strong>These findings provide a biomechanical basis for posture-guided application of oblique pulling manipulation and may assist clinicians in selecting appropriate manipulation postures for patients with lumbar disc herniation.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13465648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148711397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights on supplement use during cancer care from online Reddit conversations.","authors":"Barliz Waissengrin, Karen O'Connor, Celina H Shirazipour, Flores Amaro Ivan, Karen L Reckamp, Graciela Gonzalez-Hernandez, Jane C Figueiredo","doi":"10.1186/s12906-026-05411-5","DOIUrl":"10.1186/s12906-026-05411-5","url":null,"abstract":"<p><strong>Background: </strong>Dietary supplement use is common among patients with cancer, yet limited data exist on how patients perceive and use these products during treatment. Traditional research methods may not fully capture patient-driven motivations and experiences. Social media platforms such as Reddit provide a unique opportunity to examine real-world, unfiltered discussions about supplement use in the oncology setting.</p><p><strong>Methods: </strong>Posts from the subreddit r/cancer published between November 2010 and December 2022 that referenced dietary supplement use were identified using natural language processing (NLP) techniques. A 10% random sample (n = 155) of the identified posts (n = 1,488) was analyzed qualitatively using directed content analysis. Posts were categorized by purpose (seeking vs. reporting use), intended use (treatment of cancer vs. symptom management), and supplement type (vitamin, mineral, other). Frequencies of themes related to supplement use, treatment goals, and decision-making motivations were assessed.</p><p><strong>Results: </strong>Among users who specified their cancer type, breast, lung, and colorectal cancers were most frequently mentioned. 59% of posts were inquiries about supplement use, and 41% described personal experiences. Intended use was evenly divided between symptom management (51%) and cancer treatment (49%). Vitamins were the most commonly mentioned supplement category (38%). Reported motivations included managing fatigue, nausea, pain, and emotional distress. Discussions frequently reflected attempts to identify supportive strategies outside of conventional medical care.</p><p><strong>Conclusions: </strong>Reddit represents a valuable, underutilized resource for understanding cancer patients' perspectives on dietary supplement use. Insights derived from these online discussions highlight the importance of addressing supplement use in clinical encounters and underscore the need for further research to guide safe and effective integration of supplements into oncology care.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13499959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS/MS-based phytochemical profiling and preliminary in vitro anticancer-related bioactivity of Euphorbia petrophila ethanol extract.","authors":"Mehmet Ali Güzel, İrem Nur Menevşe, Turgay Kolaç, Şengül Yüksel, Birol Mutlu","doi":"10.1186/s12906-026-05470-8","DOIUrl":"https://doi.org/10.1186/s12906-026-05470-8","url":null,"abstract":"<p><strong>Background: </strong>Euphorbia species contain diverse secondary metabolites with reported biological activities, but Euphorbia petrophila remains pharmacologically underexplored. This study aimed to characterize the phytochemical profile of E. petrophila ethanol extract and evaluate its preliminary in vitro anticancer-related activity in lung and breast cancer cell models.</p><p><strong>Methods: </strong>Aerial parts of E. petrophila were extracted with ethanol using ultrasonic-assisted extraction. The extract was profiled by LC-MS/MS. Cytotoxicity was assessed by MTT assay in A549 and MDA-MB-231 cancer cells and in BEAS-2B and MCF10A non-tumorigenic epithelial cells. Apoptosis and cell-cycle distribution were analyzed by flow cytometry. Migration and invasion were evaluated using Transwell assays, and selected gene-expression changes were measured by RT-qPCR. Data were analyzed using appropriate analysis of variance and post hoc comparisons.</p><p><strong>Results: </strong>LC-MS/MS analysis identified phenolic acids and flavonoids, with gallic acid, quinic acid, ellagic acid, and isoquercitrin among the most abundant detected constituents. The extract reduced IC₅₀ value of in all tested cell lines, with the strongest cytotoxic effect observed in A549 cells. It increased apoptotic cell populations and promoted G0/G1 cell-cycle accumulation, particularly in A549 cells. The extract also markedly suppressed migration and invasion in A549 and MDA-MB-231 cells. These functional effects were accompanied by changes in TP53 and CDKN1A mRNA expression.</p><p><strong>Conclusions: </strong>E. petrophila ethanol extract shows preliminary antiproliferative, pro-apoptotic, anti-migratory, and anti-invasive activity in selected cancer cell models. However, because the findings are based on crude extract testing and transcript-level analyses, the results should be interpreted as preliminary and correlative. Further work should include extract standardization, LC-MS/MS method validation, broader normal-cell toxicity profiling, active-compound isolation, protein-level mechanistic assays, and in vivo safety and efficacy studies.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Awareness and attitudes toward traditional and complementary medicine among community-dwelling individuals in Türkiye: a cross-sectional online survey.","authors":"Akın Süzer, Sinem Yenil-Kocabay, Nevriye Ünal-Süzer, Nihal Büker","doi":"10.1186/s12906-026-05480-6","DOIUrl":"10.1186/s12906-026-05480-6","url":null,"abstract":"<p><strong>Background: </strong>As traditional and complementary medicine (T&CM) becomes increasingly widespread, assessing the awareness and attitudes of community-dwelling individuals is essential to understand its current status and promote its rational use. Therefore, this study aims to evaluate the awareness levels and attitudes of community-dwelling individuals in Türkiye regarding T&CM practices.</p><p><strong>Methods: </strong>This cross-sectional online survey included 1375 individuals residing in the community across Türkiye. Data were collected through a structured questionnaire via Google Forms™, including items on sociodemographic characteristics, awareness, opinions, and experiences with T&CM. Attitudes were assessed using the Complementary, Alternative and Conventional Medicine Attitude Scale (CACMAS). Data were analysed using IBM SPSS Statistics 25. Multiple linear regression analysis was performed to identify independent predictors of attitudes.</p><p><strong>Results: </strong>The most recognized T&CM practices were massage (91.13%), wet cupping (84.51%), and medicinal leech therapy (80.29%). Overall, 31.78% received T&CM information from healthcare professionals, 22.25% used it, and 60.73% recommended it. The mean CACMAS score was 108.78 ± 19.51, differing significantly by age (p = 0.004). T&CM usage varied by age, marital status (both p < 0.001), education (p = 0.006), and chronic disease (p = 0.004). Receiving T&CM information from professionals was associated with age (p = 0.029), education (p = 0.007), and perceived health (p = 0.023). Multiple linear regression revealed that increasing age (p = 0.006), female gender (p = 0.011), marital status (p = 0.049), and previous T&CM experience (p < 0.001) independently predicted favorable attitudes, whereas education (p = 0.945) and chronic disease (p = 0.218) lost significance.</p><p><strong>Conclusion: </strong>Although T&CM usage rates are relatively low, community-dwelling individuals in Türkiye exhibit generally favorable attitudes. These attitudes are independently shaped by specific sociodemographic factors, such as age and gender, rather than education or chronic disease. These findings may provide the basis for the rational use of T&CM and highlight the importance of further studies that take cultural and regional diversity into account.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnopharmacological study of medicinal plants used for breast cancer treatment among traditional herbalists and patients in Central and Western Cameroon.","authors":"Constantin Chokofi Fongho, Alfloditte Flore Feudjio, Natacha Lena Yembeau, Paul Jean Assam Assam, Etienne Okobalemba Atenguena, Constant Anatole Pieme","doi":"10.1186/s12906-026-05446-8","DOIUrl":"10.1186/s12906-026-05446-8","url":null,"abstract":"<p><strong>Background: </strong>The high diversity of ecology, climate, and floral of Cameroon constitutes a veritable reservoir of medicinal plants. The search for new, safe, effective, and inexpensive alternative therapies for the treatment of breast cancer is an urgent necessity in Africa. The aim of the study was to identify the medicinal plants recipes used by traditional herbalists to manage breast cancer diseases in four districts of the Central and Western region of Cameroon.</p><p><strong>Methods: </strong>We conducted cross sectional, qualitative and descriptive research through an ethnopharmacological survey from August 2024 to February 2025 amongst 92 participants, including 21 traditional herbalists from four districts in the Central and Western regions, and 71 patients with breast cancer disease. Traditional herbalists were selected using the snowball. A semi-structured questionnaire was used to collect data on plant-based treatment supplemented with a comprehensive literature review on the phytochemicals and therapeutic properties of these plants.</p><p><strong>Results: </strong>We have identified 29 plants species belonging to 19 families with total of 23 recipes used by the herbalists. 74% of these recipes (17) were prepared with more than one plant. The whole plant (n = 6 ;26.1%) and the bark (n = 5 ;21.7%) were the most commonly plant parts used for the recipes while the maceration was the main preparation method (n = 10;43.5%). Water was the solvent frequently used (n = 18;78.3%) and all these remedies were administrated only per os (n = 23;100%) with a dosage ranging from one and half to four glasses per day for a duration of treatment from one to twelve months. Only 10% of breast cancer patients reported being completely satisfied, unlike traditional herbalists who claim that 50% of breast cancer patients who received their treatment were cured. In most of the remedies, there was anticancer plants used as main ingredient and an adjuvant. Part of Syzygium guineense (Willd) DC, Var Guineen constitute the most frequent main ingredient (n=3/RFC=0.13) of the recipes while the most represented adjuvant (n=8/RFC=0.35 ) was Allium sativum L. The highest mean informant consensus factor for breast cancer treatment was obtained for Allium Sativum L. (0.36).</p><p><strong>Conclusion: </strong>The study reveals a wide diversity of recipes for the treatment of breast cancer in the Cameroonian pharmacopoeia.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-part specific in vitro antibacterial activity of crude ethanolic extracts of Mangifera indica against Salmonella typhi: an exploratory study.","authors":"Ivan Ronald Omara, Juliet Kyayesimira, Stephen Charles Olet","doi":"10.1186/s12906-026-05482-4","DOIUrl":"10.1186/s12906-026-05482-4","url":null,"abstract":"<p><strong>Background: </strong>Medicinal plants remain widely used in the management of infectious diseases, but evidence on the antibacterial activity of specific plant parts is often inconsistent. This study evaluated the plant-part-specific in vitro antibacterial activity of crude ethanolic extracts of Mangifera indica against Salmonella typhi.</p><p><strong>Methods: </strong>An exploratory in vitro study was conducted using crude ethanolic extracts prepared from the seed kernel, stem bark, root, and a combined extract (1:1:1 mixture) of Mangifera indica (M. indica). Antibacterial activity was assessed using the agar well diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using broth microdilution and sub-culture methods. Assays were performed in triplicate. Data were analysed using the Kruskal-Wallis test followed by Dunn's post-hoc pairwise comparisons with Holm adjustment.</p><p><strong>Results: </strong>The seed kernel extract showed the largest mean zone of inhibition (18.0 ± 0.5 mm, strong activity), followed by tetracycline (14.0 ± 0.5 mm, moderate activity) and the combined extract (11.5 ± 0.5 mm, moderate activity). Stem bark, root, and Dimethyl sulfoxide (DMSO) showed no detectable inhibition. Overall differences among treatments were statistically significant (Kruskal-Wallis test: H = 16.76, df = 5, p = 0.005). Dunn's post-hoc test showed that the seed kernel extract produced significantly larger inhibition zones than the combined extract (adjusted p = 0.0219). The seed kernel and combined extracts had MIC values of 16.67 mg/mL and MBC values of 33.34 mg/mL, with an MBC/MIC ratio of 2, indicating bactericidal activity.</p><p><strong>Conclusions: </strong>Crude ethanolic extracts of M. indica showed plant-part-specific antibacterial activity against S. typhi in vitro. The seed kernel was the most active plant part and warrants further phytochemical, toxicity, and in vivo evaluation before any therapeutic application can be inferred.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}