BMC Complementary Medicine and Therapies最新文献

{"title":"Developing a deep learning model for the automated monitoring of acupuncture needle insertion: enhancing safety in traditional acupuncture practices.","authors":"Shun-Ku Lin, Chien-Kun Su, Melnard Rome C Mercado, Syu-Jyun Peng","doi":"10.1186/s12906-025-04853-7","DOIUrl":"10.1186/s12906-025-04853-7","url":null,"abstract":"<p><strong>Background: </strong>Acupuncture is a widely practiced traditional therapy, yet safety concerns, particularly needle breakage and retention, remain critical issues that can lead to complications such as infections, organ injury, or chronic pain. This study aimed to develop a deep learning model to monitor acupuncture needle insertion, detect instances of needle breakage, and prevent needle retention, ultimately improving patient safety and treatment outcomes.</p><p><strong>Methods: </strong>A deep learning model based on the YOLOv8 architecture was trained using a dataset comprising 192 images from a commercial image library and 73 clinical images captured during real-world acupuncture sessions. Images were preprocessed through cropping and annotation, and augmented to enhance model generalizability. Five-fold cross-validation was employed to ensure robust performance. Model evaluation metrics included precision, recall, F1 score, and mean average precision (mAP) at Intersection over Union (IoU) thresholds of 50% (mAP@50) and 50-95% (mAP@50-95).</p><p><strong>Results: </strong>The model demonstrated strong performance, achieving an average precision of 88.0% and a recall of 82.9%. The mean average precision was 88.6% at mAP@50 and 62.9% at mAP@50-95, indicating high reliability in detecting acupuncture needles across diverse scenarios. These results highlight the potential of the model to enhance clinical safety by minimizing risks associated with needle breakage and retention, regardless of practitioner experience or patient demographics.</p><p><strong>Conclusions: </strong>The proposed YOLOv8-based deep learning model offers a reliable method for real-time needle monitoring in acupuncture. Its integration into clinical workflows can improve safety and efficiency, especially in underserved regions or settings with less experienced practitioners. Future research should validate the model with larger, more diverse datasets and explore its application in various healthcare settings.</p><p><strong>Trial registration: </strong>Not applicable; this study did not involve a healthcare intervention requiring registration. Data collection adhered to ethical standards with institutional approval (TCHIRB-11310004).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"108"},"PeriodicalIF":3.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the multi-target compounds of Sarcandra glabra identification and inhibition of novel target genes for the treatment of pancreatic cancer.","authors":"Xing Liu, Jianghong Ou","doi":"10.1186/s12906-025-04839-5","DOIUrl":"10.1186/s12906-025-04839-5","url":null,"abstract":"<p><strong>Background: </strong>S. glabra has been widely used to treat tumors in traditional Chinese medicine (TCM). However, the specific mechanism of action of S. glabra in pancreatic cancer remains unclear. In this study, network pharmacological analysis was used to identify the active components of S. glabra and their corresponding targets for the treatment of pancreatic cancer. Furthermore, molecular docking, molecular dynamic simulations, and in vitro experiments were performed to validate the findings.</p><p><strong>Methods: </strong>The active components of S. glabra and their corresponding targets for the treatment of pancreatic cancer were identified using the TCMSP database and a literature search. Differentially expressed genes were identified using data from the Gene Expression Omnibus (GEO) database, and their protein-protein interaction (PPI) network was constructed using the STRING platform. The target genes of S. glabra were further assessed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses in the R software. Subsequently, a protein-protein interaction (PPI) network and a composite target-pathway network were established. The target genes were subjected to survival and mutation analyses. Molecular docking and molecular dynamic simulations were used to validate the interaction between the hub target genes and S. glabra in vitro. In addition, cell viability and qRT-PCR verification of S. glabra against pancreatic cancer in vitro.</p><p><strong>Results: </strong>A total of 20 active components and 70 targets were identified. Based on the PPI network, CASP3, MMP9, CCND1, EGF, MMP2, CASP8, ERBB2, STAT1, and PPARG were identified as hub target genes. Enrichment analysis showed that S. glabra may primarily affect pathways such as p53 signaling, transcriptional dysregulation in cancer, proteoglycans in cancer, pancreatic cancer, and cell cycle. Molecular docking and molecular dynamic simulations indicated stable binding between anhydroicaritin-GSK3B and quercetin-PPARG. In vitro experiments demonstrated that treatment with S. glabra significantly inhibited the growth of PANC-1 cells and downregulated expression of GSK3B and PPARG (P < 0.05).</p><p><strong>Conclusion: </strong>This study demonstrates the potential of S. glabra, a herb in traditional Chinese medicine, for treating pancreatic cancer. The findings provide insights into the mechanism of action of the active ingredients of S. glabra, offering a strong theoretical foundation for its various clinical applications.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"106"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceptability and feasibility of online delivery of chair-based yoga for older adults with multimorbidity - lessons from a process evaluation of the gentle years yoga trial.","authors":"Lesley Ward, Laura Bissell, Jenny Howsam, Garry A Tew, Laura Wiley, Fiona Rose, Camila Sofía, Maturana Palacios, Tim Rapley","doi":"10.1186/s12906-025-04838-6","DOIUrl":"10.1186/s12906-025-04838-6","url":null,"abstract":"<p><strong>Background: </strong>Yoga is a safe, effective, and popular practice among older adults, and amenable to online delivery. The Gentle Years Yoga randomised controlled trial compared the impact of a chair-based yoga programme to usual care on the health-related quality of life of older adults with multimorbidity. This embedded, longitudinal process evaluation qualitatively explored experiences and acceptability of online delivery of the trial intervention.</p><p><strong>Methods: </strong>A subset of trial participants randomised to receive the 12-week online yoga programme, together with the trial yoga teachers, were purposively recruited to semi-structured interviews. Individual interviews were conducted via Zoom or telephone, audio-recorded, independently transcribed, and thematically analysed. Online observations were conducted of one class delivered by each teacher.</p><p><strong>Results: </strong>Eighteen yoga participants (66-91 years; 2-8 chronic health conditions) and nine teachers were interviewed once (N = 12) or twice (N = 15) from October 2020 to April 2022. Five themes predominated, common to both groups. (1) Accessibility. Reduced communication and engagement inherent to online delivery were mostly outweighed by its removal of access barriers and provision of anonymity and distraction-free environment. (2) Technology issues. While digital literacy was variable and a barrier for some, simplified access procedures and basic audiovisual instruction optimised class engagement. (3) Delivery adaptations. Key facilitation techniques included simple, repetitive instructions, increased demonstration, personalised communication, and visibility-enhancing clothing. (4) Safety. Concerns were minimal, and mostly related to restricted visual and positional information inherent to face-to-face classes. (5) Implications and implementations. Online delivery was considered viable and potentially appealing for anyone experiencing issues accessing face-to-face classes outside the home. Potential solutions to online attendance barriers included equipment loan schemes and digital learning courses using existing community-based infrastructures.</p><p><strong>Conclusions: </strong>Online chair-based yoga classes were feasible and acceptable to participants and teachers, and preferrable to face-to-face delivery by some. IT issues were minimal, and mainly resolvable through simple access processes and educational information. Accessibility advantages suggest online yoga may be suitable for a broad demographic, independent of age or health status. Establishing connections with existing health and community-based organisations presents a potential pathway for developing an equipment loan scheme to improve accessibility for those with financial access barriers.</p><p><strong>Trial registration: </strong>ISRCTN ISRCTN13567538. Registered 18 March 2019.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"107"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of garlic and stevia extract with aerobic exercise on hypothalamic leptin and ghrelin receptor mRNA expression and insulin resistance in obese rats.","authors":"Zohre Amirkhani, Armin Morteza Gholi, Sara Asghari, Danial Hakak, Mahdi Pouryousef, Behrooz Yahyaei, Sayyed-Javad Ziaolhagh","doi":"10.1186/s12906-025-04756-7","DOIUrl":"10.1186/s12906-025-04756-7","url":null,"abstract":"<p><strong>Background: </strong>Obesity has recently become the most prevalent diet-related disease worldwide. This study aimed to investigate the effects of aerobic exercise, stevia, and garlic extract on leptin and ghrelin receptor mRNA, as well as the interactions between insulin and glucose in relation to these genes.</p><p><strong>Methods: </strong>A total of 50 male Wistar rats were split into two groups: one group was fed regular rodent food, while the other was fed a high-fat diet for 12 weeks to induce obesity. The obese rats were then divided into six groups of five (N = 5) based on Lee's index: an obese control group (OC), obese treated with garlic, stevia (OGS), obese aerobic exercise (OE), obese garlic and aerobic exercise (OGE), Obese stevia and aerobic exercise (OSE), and a Normal weight group (NC). The exercise groups participated in a gradually increasing aerobic walking program, whereas the stevia and garlic groups received daily oral doses of their respective extracts. The expression of leptin and ghrelin receptor genes in the hypothalamus was measured using real-time PCR, and insulin resistance was calculated using the HOMA/IR formula. Statistical analysis was conducted using ANOVA, with a significance level set at P < 0.05.</p><p><strong>Results: </strong>The obese (O) rats experienced a significant increase in body weight compared with the control (C) rats (p = 0.013), whereas the treated rats showed no significant changes in weight. Leptin receptor mRNA levels were significantly lower in O rats than in C rats (p = 0.00), but increased significantly in the OS (p = 0.000) and OSE (p = 0.034) groups compared with obese rats. Ghrelin receptor mRNA levels were significantly higher in the O group than in the C group (p = 0.035), but decreased in all treatment groups, with the OE (p = 0.001), OGE (p = 0.001), and OSE (p = 0.004) groups showing the greatest reductions. Insulin resistance increased slightly in the O group compared with the C group (p = 0.112), but was lowest in the OS group among all groups (p = 0.018).</p><p><strong>Conclusions: </strong>Stevia significantly improved leptin and ghrelin receptor mRNA expression, glucose levels, and insulin resistance in obese rats, showing its potential as an effective dietary intervention for managing obesity.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"104"},"PeriodicalIF":3.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, antioxidant, antimicrobial, and anticancer activities of Mahonia napaulensis DC. bark from Nepal.","authors":"Rajib Kumar Shrestha, Satyabrata Nayak, Prabal Singh Maharjan, Bivek Pokharel, Gagan Deep Jhingan, Rana Pratap Singh, Neel Sarovar Bhavesh, Bhupal Govinda Shrestha","doi":"10.1186/s12906-025-04806-0","DOIUrl":"10.1186/s12906-025-04806-0","url":null,"abstract":"<p><strong>Background: </strong>Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or fewer side effects. This study aimed to assess total phenolic (TPC) and flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal, and anticancer), and chemical composition of methanol extract of M. napaulensis DC. bark (MNBM). This is the first study evaluating its anticancer activity and chemical composition by LC-MS/MS analysis.</p><p><strong>Methods: </strong>TPC, TFC, antioxidant, antimicrobial, and anticancer activities were determined by Folin-Ciocalteu, AlCl₃, DPPH, Resazurin, and MTT assays, respectively. Its metabolite profiling was done by LC-MS/MS analysis. The statistical significance of differences between test groups was analyzed by a one-way ANOVA.</p><p><strong>Results: </strong>The preliminary phytochemical screening revealed the presence of various phytochemicals viz. alkaloids, steroids, glycosides, polyphenols, tannins, flavonoids, coumarins, terpenoids, and quinone. MNBM showed 38.00 ± 1.50 mg GAE g^-1 dry sample as TPC, 35.04 ± 4.87 mg QE g^-1 dry sample as TFC, and 212.97 μg/mL IC₅₀ value (P < 0.05) as moderate antioxidant activity. MNBM showed minimal inhibitory concentration (MIC) values of 100.22 mg/mL, 50.15 mg/mL, and 25.08 mg/mL against S. aureus, E. coli, and C. albicans, respectively as weak antimicrobial activity. It showed no antibacterial effect against B. cereus and P. aeruginosa at 120 mg/mL. The anticancer activity of MNBM was moderate against human lung cancer cells A549 (228.97 μg/mL IC₅₀ value) and human cervical cancer cells HeLa (367.72 µg/mL IC₅₀ value) (P < 0.05). The LC-MS/MS analysis reported the presence of different anticancer compounds viz. dihydroberberine, d-berbamine, (S)-glaucine, protopine, grosheimin, mycophenolic acid, berberine, alpha-linolenic acid, etc. CONCLUSIONS: MNBM showed dose-dependent moderate antioxidant, weak antibacterial, weak antifungal, and moderate anticancer activity due to the synergistic effect of different phytochemicals and anticancer compounds.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"105"},"PeriodicalIF":3.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of Melissa officinalis L. essential oil inhalation on anxiety and symptom burden of hemodialysis patients: a randomized trial study.","authors":"Nilofar Pasyar, Mansoureh Aghababaei, Masoume Rambod, Mohammad M Zarshenas","doi":"10.1186/s12906-025-04840-y","DOIUrl":"10.1186/s12906-025-04840-y","url":null,"abstract":"<p><strong>Background: </strong>Hemodialysis patients suffer from physical and mental symptoms for which aromatherapy might be useful. This study aimed to determine the effectiveness of Melissa officinalis L. (Lemon Balm) essential oil inhalation on anxiety and the burden of symptoms in hemodialysis patients.</p><p><strong>Methods: </strong>This study is a randomized clinical trial with a pretest-posttest design. Sixty-eight hemodialysis patients were randomly divided into intervention (who underwent Melissa officinalis L. inhalation aromatherapy 3 times a week for one month) and control (who inhaled refined sweet almond oil) groups. The intervention in both groups was conducted during the hemodialysis session. The data were collected using the state-trait anxiety inventory and Dialysis Symptom Index. Data were analyzed through SPSS-25, using Mann-Whitney, Wilcoxon, t-tests and MANOVA.</p><p><strong>Results: </strong>Before the intervention, both groups were similar in terms of anxiety and symptoms burden (P > 0.05). However, after the intervention, a significant difference was found between the groups in terms of state and trait anxiety, and severity of constipation, nausea, vomit, diarrhea, swelling in legs, muscle cramps and shortness, worry, nervousness and anxiety, and trouble staying asleep. Moreover, after the intervention, a significant difference was observed between the groups regarding the prevalence of symptoms burden such as feeling anxious and having a dry mouth (P < 0.05).</p><p><strong>Conclusion: </strong>Melissa officinalis L. essential oil inhalation aromatherapy reduced the symptoms burden and anxiety in hemodialysis patients. Given the effectiveness of aromatherapy in hemodialysis patients, it is suggested that healthcare workers should use this complementary and integrative health to reduce the anxiety and symptoms burden in hemodialysis patients.</p><p><strong>Trial registration rct registry: </strong>Iranian Registry of Clinical Trials (IRCT) number: IRCT20191021045178N3; Registration date 27/04/2021.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"103"},"PeriodicalIF":3.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-reported therapeutic benefits of herbal medicinal products in the treatment of gynecological ailments.","authors":"Alexandra Drebka, Annika J Scholl, Teresa Ochs, Olaf Kelber, Ralph Mösges, Esther Raskopf, Kija Shah-Hosseini, Beatrice E Bachmeier","doi":"10.1186/s12906-025-04761-w","DOIUrl":"10.1186/s12906-025-04761-w","url":null,"abstract":"<p><strong>Background: </strong>Gynecological ailments have a negative impact on quality of life and productivity. Standard treatment is associated with poor tolerability and other issues related to public health and environment. Herbal Medicinal Products (HMPs) are used traditionally for the treatment of menstrual and menopausal ailments as well as uncomplicated urinary tract infections (uUTIs) for centuries and constitute a suitable addition to current treatment options. HMPs are well tolerated, non-polluting and therapeutically efficacious as evidenced by various clinical studies. Aim of this study was to expand the evidence regarding therapeutic effectiveness of HMPs for the treatment of gynecological complaints by complementing knowledge from clinical studies with real-world evidence from patient-reported outcomes.</p><p><strong>Methods: </strong>A data set consisting of patient-reported outcomes regarding the treatment of gynecological ailments (n = 1658) with HMPs was taken from the pharmaco-epidemiological database PhytoVIS. After data preparation excluding all cases of herbal supplements, homeopathic preparations, or non-herbal medicinal products the remaining data (n = 1363) was grouped into the three indications menstrual complaints (n = 222), menopausal complaints (n = 301), and uUTIs (n = 840). We applied descriptive statistical methods (frequency and percentage) with regard to the variables \"age\", \"treatment duration\", \"severity of symptoms\", \"therapeutic benefits\", and \"adverse drug reactions\". Thereafter we evaluated the therapeutic benefit of HMPs as well as adverse events.</p><p><strong>Results: </strong>The majority of the patients (82.2%) in the sample assessed the overall therapeutic effect of HMPs for the treatment of gynecological complaints as beneficial and 90.8% of them perceived no or no significant adverse events. Treatment habits differed depending on the type of complaint. In this context the majority of women with menstrual or menopausal ailments preferred to treat for time period of 1 month or longer, while those affected by uUTIs reduced the application of HMPs to the length of their symptoms. Interestingly women with even strong symptoms relied on the therapeutic benefit of HMPs.</p><p><strong>Conclusion: </strong>Real-world outcome data are an important supplement to clinical data. Our results reveal a favorable benefit-risk ratio of HMPs and help to implement them into novel therapeutic strategies to treat gynecological complaints.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"99"},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis, antioxidant activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells.","authors":"Suwisit Manmuan, Thanchanok Sirirak, Sukannika Tubtimsri, Arpa Petchsomrit, Tiraniti Chuenbarn","doi":"10.1186/s12906-025-04835-9","DOIUrl":"10.1186/s12906-025-04835-9","url":null,"abstract":"<p><strong>Background: </strong>Marine algae have excellent phytoconstituents with notable biological activity and bioactive therapeutic benefits, but the anti-oral cancer activity of Caulerpa lentillifera (C. lentillifera) has not been widely studied. This study aimed to explore the anti-cancer properties of C. lentillifera to gain insights into possible treatment approaches.</p><p><strong>Methods: </strong>The three C. lentillifera extracts were prepared using the maceration method with methanol (CLM), ethanol (CLE), and acetone (CLA). The chemical composition of extracts of C. lentillifera was investigated. Its metabolite profiles were selectively further investigated using the LC-QTOF MS/MS technique and their antioxidative activity was evaluated. The cytotoxic effect on KON cells and MRC-5 cells was assessed using the MTT test. Morphological changes and apoptosis were examined through Hoechst 33,258 and AO double staining, while DAPI and FDA double labeling were used to observe the nucleus and cytoplasm. Using a flow cytometer, the percentage of cell cycle arrest was calculated and the fraction of cell death was examined.</p><p><strong>Results: </strong>The CLA exhibited higher quantities of TPC, TFC, chlorophyll a, and chlorophyll b compared to the CLM and CLE. The LC-QTOF MS/MS analysis revealed ten major phytochemicals in the CLA. The three C. lentillifera extracts exhibited antioxidative activity, with the CLE demonstrating significantly higher antioxidant activity compared to the CLM and CLA. In-vitro, the KON oral cancer cells exhibited sensitivity to CLA, CLE, and CLM in that order. The three extracts induced ROS-mediated cell death as well as disruption of mitochondrial membrane potential, with concentrations at IC₄₀, IC₆₀, and IC₈₀ leading to apoptosis within 24 h. Furthermore, the cell cycle of KON cells was blocked in sub-G and G0-G1 by all three extracts. Notably, the extracts significantly impeded colony growth, migration, and invasion. The increase in cellular uptake was measured using the TEER test.</p><p><strong>Conclusion: </strong>The findings showed that C. lentillifera has several functional metabolites, antioxidative activity, and strong anti-tumor properties. According to these results, C. lentillifera extracts may be utilized to treat oral cancer.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"101"},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of aromatherapy with lemongrass (Cymbopogon citratus) on the anxiety of patients undergoing scaling and root planning: a randomized clinical trial.","authors":"Fahimeh Rashidi Maybodi, Vahid Herandi, Mahta Sadat Vaezpour","doi":"10.1186/s12906-025-04834-w","DOIUrl":"10.1186/s12906-025-04834-w","url":null,"abstract":"<p><strong>Background: </strong>Anxiety is one of the most common factors that prevent people from going to a dentist. Therefore, finding a solution to better control stress has been an important issue in recent studies. Lemongrass positively affects the prevention of dental anxiety. The literature has shown that aromatherapy may regulate hemodynamic factors in patients. This study investigated the effects of the aroma of lemongrass on anxiety levels and hemodynamic factors during nonsurgical periodontal treatment.</p><p><strong>Methods: </strong>Thirty-eight patients were divided into two groups. First, the patients were asked to complete the Spielberger questionnaire to measure their level of dental anxiety. Blood pressure, heart rate, oxygen saturation, pain and satisfaction with treatment were evaluated. In the case group, nonsurgical periodontal therapy was performed while the patient inhaled the aroma of the lemongrass. Scaling was performed without intervention in the control group. The patients were then asked to complete the questionnaire again, and all the variables mentioned were remeasured.</p><p><strong>Results: </strong>Aromatherapy with lemongrass caused significant reductions in the systolic and diastolic blood pressure values, heart rate, and anxiety scores of patients. On the other hand, the differences in blood oxygen saturation changes, amount of pain and treatment satisfaction were insignificant between the two groups.</p><p><strong>Conclusion: </strong>Inhalation of lemongrass essential oil appears beneficial for reducing anxiety and associated hemodynamic changes. Thus, aromatherapy with lemongrass may alleviate dental patients' anxiety.</p><p><strong>Trial registration: </strong>https://irct.behdasht.gov.ir/ IRCT20230615058492N2, 21/02/2024.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"100"},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactives derived from Brazilian native flora with antimicrobial and anticancer activity.","authors":"Daniele Cristina Vitorelli-Venancio, Rosemary Matias, Amanda Rodrigues Ganassin, Fabio Antonio Venancio, Renata Trentin Perdomo, Giovana Bicudo Gomes, Angela Kwiatkowski, João Víctor de Andrade Dos Santos, Marilene Rodrigues Chang","doi":"10.1186/s12906-025-04787-0","DOIUrl":"10.1186/s12906-025-04787-0","url":null,"abstract":"<p><strong>Background: </strong>The development of new drugs that act against multidrug-resistant microorganisms and malignant tumors is necessary owing to the limited therapeutic options and high mortality rates associated with these pathologies. In this study, we evaluated the phytochemical groups present in seven plants from the Brazilian Cerrado even as their antioxidant, antiproliferative and antimicrobial activities.</p><p><strong>Methods: </strong>The extracts were obtained by the maceration technique and secondary metabolites were determined by phytochemical analysis. The antioxidant activity was assessed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method. The antiproliferative activity of the extracts was assessed using human breast, kidney, and liver neoplastic cells. Cytotoxicity was evaluated in a non-neoplastic cell line - NIH/3T3. The antimicrobial activity of the plant extracts against resistant bacteria and yeasts was determined using disk diffusion assays, and the minimum inhibitory concentration (MIC) was determined by the broth microdilution technique.</p><p><strong>Results: </strong>Phytochemical analysis revealed the presence of phenolic compounds, flavonoids, steroids, tannins, and saponins in all of the extracts, with Smilax fluminensis showing the highest levels of phenolic compounds and flavonoids. All tested extracts exhibited antioxidant activity above 50%, notably Tapiria obtusa (82.36 ± 0.44). The T. obtusa extract showed potent antiproliferative activity against the 786-0 cell line (GI₅₀ 10.16 ± 2.33 µg/mL) and a significantly greater SI (SI = 24.61) than the control (SI = 3.23, doxorubicin), indicating its selective cytotoxicity against cancer cells and its potential as a therapeutic agent against renal cancer. No cytotoxicity was observed in non-tumor cells. Extracts of S. fluminensis leaves showed fungicidal effects on Candida glabrata (MIC = 500 µg/mL). This study is the first to demonstrate the antibacterial activity of T. obtusa leaf ethanolic extract against MRSA (MIC = 1,000 µg/mL).</p><p><strong>Conclusions: </strong>The ethanolic extract of T. obtusa demonstrated antioxidant activity, antiproliferative effects against the 786-0 cell line, and antibacterial activity against MRSA. The ethanolic extract of S. fluminensis leaves exhibited a fungicidal effect against C. glabrata. These findings may pave the way for more effective and safer treatments for managing oncological and infectious diseases.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"102"},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11900633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}