BMC Complementary Medicine and Therapies最新文献

{"title":"An investigation on anti-biofilm potential of Aegle marmelos fruit extract against multi-drug-resistant Staphylococcus aureus.","authors":"Debarati Jana, Tuhin Manna, Kartik Chandra Guchhait, Sahadeb Panja, Amit Karmakar, Saroj Ballav, Subrata Hazra, Subhamoy Dey, Amiya Kumar Panda, Chandradipa Ghosh","doi":"10.1186/s12906-025-05062-y","DOIUrl":"10.1186/s12906-025-05062-y","url":null,"abstract":"<p><strong>Background: </strong>Staphylococcus aureus, member of ESKAPEE pathogens is a noteworthy contributor to the global crisis rising due to antimicrobial resistance. Biofilms are the primary reason behind the increased antibiotic resistance and tolerance of pathogens. Hence targeting bacterial biofilms has been prioritized as an alternative strategy to counter antibiotic resistance. Aegle marmelos has gained prominence in Indian traditional medicine as seeds, fruits, leaves, bark and roots of this plant are being in use extensively in treating several kinds of ailments by the inhabitants of this subcontinent due to its ethno-pharmacological relevance. The fruit of this plant has been found with remarkable anti-bacterial properties along with other therapeutic efficacies. The present study aimed to identify the anti-biofilm potential of methanolic fruit extract of Aegle marmelos (AMFE) against multi-drug-resistant (MDR) S. aureus strains as a resort to counter the global crisis of antimicrobial resistance for alternative approaches.</p><p><strong>Results: </strong>MBIC and MBEC of AMFE ranged between 100 and 200 µg.mL^-1 and 300-500 µg.mL^-1, respectively. AMFE could substantially reduce the carbohydrate and protein content of the exo-polymeric substance (EPS), crucial for biofilm production. Expressions of major biofilm promoting genes icaAD and its accessory sarA were down-regulated upon AMFE treatment as revealed from qRT-PCR analysis whereas the quorum sensing gene agr that promotes biofilm detachment was up-regulated. Fluorescence, scanning electron and atomic force microscopic studies confirm the reduction of biofilm biomass upon AMFE treatment. Up to 10 mg.mL^-1 AMFE was non-toxic to human lymphocytes with cell viability of 75.35%. GC-MS and FT-IR studies could detect the bioactive components where 9-octadecenoic acid, n-hexadecanoic acid, 9,12-octadecadienoic acid, methyl 4,7,10- hexadecatrienoate were the major components.</p><p><strong>Conclusion: </strong>Anti-biofilm activity of AMFE towards MDR S. aureus have been established through in vitro biochemical and gene expression studies that were further substantiated by microscopic studies which reveal that AMFE could be explored in the management of S. aureus-associated infections.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"334"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic overcoming of cisplatin resistance in ovarian cancer by combined Astragalus Membranaceus and cisplatin treatment: network pharmacology and experimental validation.","authors":"Fei Wang, Qing-Fang Yue, Yu Zhang, Fang-Liang Lei","doi":"10.1186/s12906-025-05066-8","DOIUrl":"10.1186/s12906-025-05066-8","url":null,"abstract":"<p><p>Ovarian cancer (OC) is a leading cause of mortality among gynecologic malignancies. Cisplatin (DDP) is a first-line chemotherapy agent, but resistance to DDP often develops, compromising its efficacy. Astragalus membranaceus (AS), a traditional Chinese medicine, has shown promise in enhancing chemotherapy sensitivity due to its anti-inflammatory and immunomodulatory properties. This study investigates the potential of AS to overcome DDP resistance in OC. We integrated multiple independent DDP-resistant OC datasets identified 337 DDP resistance-associated targets. Network pharmacology identified 20 active compounds in AS, with 22 potential targets related to DDP resistance. GO and KEGG analyses revealed enrichment in pathways involving inflammation and cell adhesion. Survival analysis indicated nine genes significantly associated with OC prognosis and immune infiltration. Molecular docking showed strong binding affinities between AS compounds and these targets. In vitro, assays demonstrated that AS combined with DDP significantly inhibited cell proliferation and migration while inducing apoptosis in DDP-resistant OC cells. Western blot analysis confirmed significant changes in critical proteins (IL1B, IL1A, SERPINE1, ITGA2, and AXL) with combined treatment. AS combined with DDP significantly enhances the inhibition of cell proliferation and migration while promoting apoptosis in DDP-resistant OC cells. These findings suggest that AS could be a valuable adjunct to DDP in overcoming chemoresistance in OC, potentially improving patient outcomes.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"337"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rf improves glucose metabolism via the IRS/PI3K/Akt and PPARα/PGC1α signaling pathways in insulin-resistant AML12 cells.","authors":"Seulmin Hong, Jangho Lee, Sang Yoon Choi, Jae-Ho Park, Yu Geon Lee","doi":"10.1186/s12906-025-05091-7","DOIUrl":"10.1186/s12906-025-05091-7","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"340"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy and safety of herbal medicines for glycaemic control and insulin resistance in individuals with type 2 diabetes: an umbrella review.","authors":"Ethan Li, Alison Maunder, Jing Liu, Chhiti Pandey, Adele Cave, Allana O'Fee, Carolyn Ee","doi":"10.1186/s12906-025-05059-7","DOIUrl":"10.1186/s12906-025-05059-7","url":null,"abstract":"<p><strong>Aim: </strong>The global prevalence of diabetes is increasing rapidly. While glycaemic control remains the cornerstone of type 2 diabetes (T2DM) management, hypoglycaemic medications are associated with side effects. Many individuals with T2DM use herbal medicines despite potential risks. This review aims to comprehensively evaluate the efficacy and safety of herbal medicines for T2DM.</p><p><strong>Methods: </strong>Four electronic databases were searched from August 2018-August 2023 for umbrella reviews, meta-analyses, and systematic reviews on herbal medicine for T2DM. The primary outcome was glycaemic control assessed by glycated haemoglobin (HbA1c); secondary outcomes included measures of insulin resistance, quality of life and adverse events. Quality assessment was performed using A Measurement Tool to Assess Systematic Reviews Version 2 (AMSTAR 2) and certainty of evidence for the primary outcome was assessed using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach.</p><p><strong>Results: </strong>Eight meta-analyses and two systematic reviews were included. Ginger, turmeric, and Jinlida granules significantly decreased HbA1c compared to placebo (mean differences (MD): -0.467%, -0.486%, -0.283%, respectively) with moderate to high certainty evidence. Berberis vulgaris, Gegen Qinlian decoction and pycnogenol may potentially decrease HbA1c (very low certainty evidence). Ginger, turmeric, cardamom, Berberis vulgaris, Nigella sativa, and various Chinese herbal medicines demonstrated improvements in insulin resistance measures. However, overall confidence in these findings is limited due to the low to very low quality of the included reviews. Few mild adverse effects were reported, but data was incomplete.</p><p><strong>Conclusions: </strong>Current evidence supports the use of ginger and turmeric for glycaemic control in type 2 diabetes, however, given the high clinical heterogeneity and low quality of the review, our confidence in this finding is somewhat limited. Herbal medicines should be used only as an adjunct to conventional treatment, with shared decision-making between clinicians and patients. Careful monitoring for hypoglycaemia is essential, particularly when herbal medicines are used concurrently with antidiabetic drugs, given a current lack of data on herb-drug interactions. Future research should clarify optimal regimens, safety profiles, and include diverse populations to enhance generalisability.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"341"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herb-drug interaction of Astragali Radix based on in vitro incubation and pharmacokinetic assessment.","authors":"Tianwang Wang, Tingting Zhang, Xiaofei Chen, Chonggang Huang, Pengfei Tu, Kai Wang, Feng Qiu","doi":"10.1186/s12906-025-05085-5","DOIUrl":"10.1186/s12906-025-05085-5","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"339"},"PeriodicalIF":3.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanocarriers loaded with Egyptian Calligonum comosum L'Hér. Extract: an eco-friendly approach for investigating triple-action biological activities.","authors":"Yasser I Khedr, Soliman M Toto, Salama M El-Darier, Mostafa K Hafez, Abdel-Hamid A Sakr, Magdi A Ali, Mohamed Zakaria El-Sayed, Aya M Helal","doi":"10.1186/s12906-025-05047-x","DOIUrl":"10.1186/s12906-025-05047-x","url":null,"abstract":"<p><strong>Background: </strong>Calligonum comosum L'Hér. is a large perennial shrub that grows in the desert. This plant inhabits much of the Egyptian desert, but has not yet been scientifically validated. Herein, we demonstrate the antimicrobial, anti-inflammatory, antioxidant, and anticancer properties of the ethanolic extract of the Egyptian C. comosum (CE) and its novel eco-friendly chitosan nano-formula (CE/CsNPs), which is prepared using citrate as a crosslinker.</p><p><strong>Methods: </strong>The produced plant ethanolic extract (CE) was chemically analyzed. The eco-friendly CE/CsNPs were successfully developed through the conjugation of CE with different concentrations (200, 350, and 400 mg/100 mL) within the chitosan nanoparticle; then, it was fully characterized for the determination of the optimum formula and followed by the assessment of the antioxidant effects, proinflammatory cytokines, evaluation of cytotoxicity against different types of cancer cell lines, and estimation of antimicrobial effects of CE and CE/CsNPs.</p><p><strong>Results: </strong>The eco-friendly CE/CsNPs-2 encapsulating 350 mg of CE was selected for the in vitro studies, as it displayed NPs with a small size and a surface charge of 37.7 ± 1.91 mV with the best encapsulation efficiency (70.15 ± 1.58%) within the CsNPs, and it showed a good release profile. Transmission electron microscope (TEM) images confirmed the discrete spherical shape of CE/CsNPs-2, with no aggregations, and revealed a size range of 49.69 to 71.93 nm. FTIR displayed the successful incorporation of CE into the eco-friendly CsNPs. Regarding their biological activity, CE/CsNPs have superior efficacy with high cytotoxicity against cancer cell lines and antioxidant effects. RT-qPCR gene expression assessments displayed that CE/CsNPs resulted in a remarkable downregulation of the inflammatory cytokines' mRNA transcript levels (p < 0.05). It enhanced the antimicrobial effect, with an inhibition zone of 12.34 ± 0.75 mm for Escherichia coli, 16.41 ± 1.01 mm for Staphylococcus aureus, and 18.74 ± 1.13 mm for Candida albicans.</p><p><strong>Conclusions: </strong>The CE possesses various biological activities, and the developed CE/CsNPs could serve as an appealing formulation to be further investigated for use in anticancer regimens.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"332"},"PeriodicalIF":3.4,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12459056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aqueous extract of Peristrophe bivalvis leaf alleviates oxidative stress in L-NAME-induced hypertensive rats.","authors":"Esther Oluwasola Aluko, Wahab Adekunle Oyeyemi, Adesoji Adedipe Fasanmade","doi":"10.1186/s12906-025-05071-x","DOIUrl":"10.1186/s12906-025-05071-x","url":null,"abstract":"<p><strong>Background: </strong>Hypertension, known as the silent killer, is associated with several complications, of which oxidative stress is thought to be one of the major fundamental mechanisms causing these complications. Conversely, Peristrophe bivalvis (PB) (L.) Merr. is utilized in alternative medicine to address hypertension, diabetes, and various other conditions. Studies have indicated its blood pressure-lowering effect in different models of hypertension; furthermore, its in vitro antioxidant activities have also been reported. However, there is a paucity of data regarding its in vivo antioxidant properties. This research investigated the impact of the aqueous extract of PB leaf (APBL) on oxidative stress induced by hypertension in rats, which was triggered by NG-Nitro-L-arginine methyl ester (L-NAME).</p><p><strong>Methods: </strong>Thirty male Wistar rats were randomly allocated to six different groups (n = 5 each): control group (10 mL/kg distilled water [D10]), hypertensive sacrificed in week 8 group (euthanized in the eighth week), untreated hypertensive group (60 mg/kg L-NAME [L60] + D10, [H]), hypertensive recovery group (D10), standard drug-hypertensive group (L60 + 10 mg/kg ramipril), and APBL-hypertensive group (L60 + 200 mg/kg APBL [APBL^H]). Blood pressure and serum levels of malondialdehyde, superoxide dismutase, glutathione, angiotensin II, and endothelin 1 were measured. Heart histology was examined to determine L-NAME hypertension severity.</p><p><strong>Results: </strong>The results indicated a notable reduction in blood pressure (SBP: 148 ± 2.28 vs. 196 ± 4.47; DBP: 110 ± 6.69 vs. 158 ± 6.22; and MAP: 171 ± 4.30 vs. 123 ± 4.66 mm Hg, p < 0.05), malondialdehyde (1.40 ± 0.14 vs. 9.26 ± 1.06 µM/mg protein, p < 0.05), angiotensin II (29.40 ± 1.34 vs. 51.34 ± 2.28 pmol/L, p < 0.05), and endothelin-1 (86.42 ± 5.88 vs. 122.60 ± 6.37 pg/mL, p < 0.05) levels in APBL^H when compared to H. The levels of superoxide dismutase (2.38 ± 0.34 vs. 0.29 ± 0.05 U/mg protein, p < 0.05) and glutathione (1.32 ± 0.47 vs. 0.30 ± 0.10 mM/mg protein, p < 0.05) were markedly elevated in APBL^H in comparison to H. Furthermore, the APBL^H group demonstrated an improvement in heart histopathology compared to the H group.</p><p><strong>Conclusions: </strong>Conclusively, the results of this research indicate that APBL significantly mitigated the oxidative stress caused by hypertension in rats. This antioxidant effect is likely due to its capacity to lower levels of endothelin-1 and angiotensin II, both of which are recognized for their role in inducing oxidative stress by augmenting the formation of reactive oxygen species.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"333"},"PeriodicalIF":3.4,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12462060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of prophylactic and therapeutic activity of camel whey protein and its trypsinized hydrolysate against experimental trichinellosis.","authors":"Sara A Abdel Salam, Aisha El-Attar, Marwa Abdelaziz, Hoda A Rashed","doi":"10.1186/s12906-025-05083-7","DOIUrl":"10.1186/s12906-025-05083-7","url":null,"abstract":"<p><strong>Background: </strong>To date, control of trichinellosis has been dependent on the conventional chemotherapeutic agent, albendazole (ALB), with its hitherto unsolved flaws, including a serious adverse profile, minor activity against muscle larva (ML) stages, and resistance development. Camel whey protein (CWP), a unique non-pharmaceutical nutraceutical, has altered the treatment trajectory of a plethora of pathological conditions. The present study aimed to evaluate the prophylactic and therapeutic profile of CWP and its tryptic hydrolysate (TWH) against experimental intestinal and muscular trichinellosis.</p><p><strong>Methods: </strong>CWP and TWH were characterized by free amino acids analysis using the HPLC Pico-Tag method and DPPH free radical scavenging antioxidant assay. 200 µl of five-day oral dosing of CWP and its TWH was prophylactically and therapeutically administered to mice. Parasitological, ultrastructural, biochemical, and histopathological studies were performed for assessment of their anti-trichinella activity. Preclinical data were subjected to analysis of variance and a Tukey post hoc test for pairwise comparisons.</p><p><strong>Results: </strong>The free amino acids profile and high DPPH scavenging antioxidant activity of CWP (79.2%) and TWH (82.7%) were determined. Parasitologically, TWH showed the highest prophylactic (84.0%R and 83.8%R) and therapeutic (94.6%R and 93.9%R) activity in mean worm count recovered from intestinal and muscular stages, respectively. Lesser reductions were recorded by CWP, whether prophylactically-treated (73.9%R and 72.1%R) or therapeutically-treated (84.5%R and 83.9%R) against aforementioned stages, respectively. In addition, scanning electron microscopy revealed that the most severe ultrastructural deformities were observed in TWH-treated worms. Biochemically, the prophylactic and therapeutic administration of TWH recorded the highest antioxidant serum level of reduced glutathione (GSH) that curbed the oxidant malondialdehyde (MDA). Histopathologically, TWH ameliorated the intestinal and muscular pathology compared to CWP.</p><p><strong>Conclusions: </strong>To the best of our knowledge, this is the first study on the prophylactic and therapeutic administration of CWP and TWH against experimental trichinellosis, showing the superiority of TWH in inducing multistage activity and ameliorating the intestinal and muscular pathology.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"331"},"PeriodicalIF":3.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145123984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline individual factors associated with clinical outcomes in adults with non-specific low back pain following manual therapy: a systematic review.","authors":"Gaetan Barbier, Mathieu Picchiottino, Arnaud Delafontaine, Guillaume Goncalves, André Bussières, François Cottin, Arnaud Lardon","doi":"10.1186/s12906-025-04975-y","DOIUrl":"10.1186/s12906-025-04975-y","url":null,"abstract":"<p><strong>Background: </strong>Primary care providers consider the identification of patient subgroups as a high research priority. Unfortunately, evidence to support the benefit of treatments targeting subgroups of patients with NSLBP remains inconsistent. Specifically, little is known about baseline individual patient characteristics associated with optimal clinical improvement from manual therapy. This systematic review aims to identify baseline individual factors (BIFs), including patient characteristics, self-reported questionnaires, clinical examination, and ancillary test factors associated with clinical improvement (or lack of) among adult patients with Non-Specific Low Back Pain (NSLBP) following manual therapy.</p><p><strong>Methods: </strong>A systematic review of published evidence in Medline, Embase, Cochrane, Index To Chiropractic Literature, and CINAHL was conducted until April 2024. Studies included participants aged 18 years and over with NSLBP and without radiculopathy. Participants received manual therapies, including musculoskeletal manipulation/mobilization (spinal and extremities) and soft tissue therapy. We excluded mechanically assisted manipulations and interventions mainly involving exercise, education, and/or advice. Two independent assessors screened studies for inclusion, extracted data, and assessed risks of bias using the Quality In Prognosis Studies (QUIPS) Tools. A qualitative synthesis of findings was undertaken. BIFs were synthesized according to patient-reported outcomes measure domains: 1) pain intensity measures, 2) disability measures, 3) global perceived effect, and 4) other factors (e.g., satisfaction with care, total number of visits).</p><p><strong>Results: </strong>Data from 19 studies (reported in 21 articles) involving 4,689 participants were analyzed. Twelve studies reported pain intensity, 18 reported disability outcomes, and 4 reported patient's global perceived effect. Over 70% of the included studies had a high risk of confounding bias. Included studies explored the potential association between clinical outcomes and 172 BIFs. BIFs were categorized into patient characteristics (n = 40), self-reported questionnaire (n = 31), clinical examination (n = 82), and ancillary tests (n = 20). Fourteen multivariate models explored the association with clinical improvement, and four others investigated the association with non-improvement. Findings were inconsistent across studies.</p><p><strong>Conclusion: </strong>Using BIFs in clinical practice to predict clinical outcomes following manual therapy treatment appears to be premature. Future studies should aim to replicate the results and differentiate prognostic factors from treatment effect modifiers.</p><p><strong>Trial registration: </strong>CRD42019131416.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"330"},"PeriodicalIF":3.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine actinobacterium Streptomyces vinaceusdrappus mediated nano-selenium: biosynthesis and biomedical activities.","authors":"Ahmed Ghareeb, Amr Fouda, Rania M Kishk, Waleed M El Kazzaz","doi":"10.1186/s12906-025-05073-9","DOIUrl":"10.1186/s12906-025-05073-9","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"329"},"PeriodicalIF":3.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}