BMC Complementary Medicine and Therapies最新文献

{"title":"The effects of Shilajit on periodontal ligament cells in wound healing: a comprehensive in vitro study.","authors":"Abdullah Alqarni, Jagadish Hosmani, Rayan Mohammedfarooq Meer, Abdulwahab Alqarni, Abdullah Alumudh, Elumalai Perumal, Mohmed Isaqali Karobari","doi":"10.1186/s12906-025-04833-x","DOIUrl":"10.1186/s12906-025-04833-x","url":null,"abstract":"<p><strong>Background: </strong>Shilajit is a pale-brown to blackish-brown fluid that varies in consistency and is released from rock layers in various mountain ranges across the world. For thousands of years, traditional medical systems in several nations have included shilajit in one form or another as a rejuvenator and adaptogen. Numerous medicinal qualities have been attributed to it, several of which have been confirmed by contemporary scientific analysis. This in vitro study was established to investigate the effect of shilajit on human Periodontal ligament (hPDL) cell wound closure.</p><p><strong>Methods: </strong>The cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) test following a 24-hour exposure of shilajit. With the use of an inverted phase contrast microscope, morphological alterations were noted. Acridine orange/ethidium bromide dual labeling, to evaluate the apoptotic cell death in shilajit treated cells. An in vitro wound healing test was utilized to evaluate wound healing in wounded hPDL cell monolayers for 24 h in the presence or absence of shilajit. The Matrix metalloproteinases-2 and 9 (MMP-2 and MMP-9) in hPDL cells treated with shilajit were measured using the enzyme-linked immunosorbent assay (ELISA) technique, and real-time PCR was used to examine gene expression linked to wound healing and apoptosis.</p><p><strong>Results: </strong>Shilajit's cytotoxicity evaluation on hPDL cells showed that dosages as high as 3 mg/mL had no adverse effects and maintains the cell viability, suggesting a possible stimulatory effect on cell growth. Cell viability was reduced significantly (p < 0.05) by dosages more than 4 mg/mL, indicating cytotoxicity at higher concentrations. According to the scratch wound healing assay, shilajit administration at doses of 2 and 3 mg/mL accelerated wound healing and improved cell migration in hPDL cells. Shilajit promoted a controlled inflammatory response and supported periodontal ligament healing by upregulating the expression of genes involved in collagen synthesis, collagen type I alpha 1 chain (COL1A1) and pro-inflammatory cytokines, tumor necrosis factor alpha (TNF-α) and, interleukin-1-beta (IL-1β), according to real-time PCR data. In addition, Shilajit raised the protein levels of MMP2 and MMP9, two important enzymes involved in tissue remodeling. Shilajit-treated hPDL cells showed a substantial increase of cell proliferation and no discernible apoptotic activity.</p><p><strong>Conclusions: </strong>Our research offers novel proof that shilajit promotes hPDL cell migration and proliferation, which in turn promotes wound closure. According to these results, Shilajit may improve tissue regeneration, accelerate wound healing, and encourage the growth of periodontal ligament cells.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"98"},"PeriodicalIF":3.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoids of Andrographis paniculata regulate hepatitis B virus replication and hepatocellular carcinoma progression: evidence from computational and experimental studies.","authors":"Vishal S Patil, Darasaguppe R Harish, Rajitha Charla, Vishwambhar Vishnu Bhandare, Swarup S Gujarathi, Faizan A Beerwala, Priyanka P Patil, Sunil S Jalalpure, Harsha V Hegde, Subarna Roy","doi":"10.1186/s12906-025-04807-z","DOIUrl":"10.1186/s12906-025-04807-z","url":null,"abstract":"<p><strong>Background: </strong>The HBx protein of hepatitis B virus (HBV) plays a crucial role in HBV pathogenesis, yet current treatments like HIV reverse transcriptase (RT) inhibitors, which target HBV RT due to similar active sites, have severe side effects, risk of drug resistance, and high costs. The present study investigates the anti-hepatitis B virus (HBV) properties of Andrographis paniculata (AP) and Thespesia populnea (TP) on HBV expressing HepG2.2.15 cells and by computational analysis.</p><p><strong>Methods: </strong>In vitro cytotoxicity, reverse transcriptase inhibitory, DNA and pgRNA quantification by qRT-PCR, time course analysis of HBsAg and HBeAg, and HBX-HBXIP interaction inhibition studies were conducted. The interaction of HBX with HBXIP, and phytocompounds' interaction with HBx was analyzed through molecular docking and dynamics studies.</p><p><strong>Results: </strong>AP exhibits lower cytotoxicity (CC₅₀ = 832.915 µg/mL) than TP (CC₅₀ = 593.122 µg/mL) after 24 h, with Tenofovir disoproxil fumarate (TDF) showing minimal cytotoxicity (CC₅₀ > 500 µM). Both AP and TP significantly decreased intracellular HBV DNA with a > 2⁵ fold reduction at higher concentrations (125-500 µg/mL) but had no significant effect on pgRNA level. AP and TP 500 µg/mL effectively inhibited HBsAg secretion (95% and 80% inhibition, respectively), over 120 h. AP also showed inhibition of HBeAg secretion (75-82%), while TP exhibited a higher inhibition of 90% at 24 h. TDF showed consistent but lower inhibitory effects on HBsAg and HBeAg. The HBx-HBXIP interaction inhibition assay showed AP's greater inhibitory capacity (IC₅₀ < 62.5 µg/mL) compared to TP (IC₅₀ = 806.69 µg/mL). Computational studies further validated these findings, showing stable binding interactions of AP compounds (flavonoids) with HBx protein (with Arg138 and His139, Lys140, and Trp141 residues participating in the interaction with HBXIP), corroborating their potential in disrupting HBV replication. Molecular dynamics simulations confirmed the stability of these interactions over 100ns.</p><p><strong>Conclusions: </strong>AP exhibits potent anti-HBV activities, making it a promising candidate for further therapeutic development.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"95"},"PeriodicalIF":3.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holistic horizons: understanding the landscape of traditional and complementary medicines among menopausal women in Kuala Lumpur.","authors":"Evelyn Xiao Xin Lau, Muhammad Junaid Farrukh, Fazlollah Keshavarzi, Long Chiau Ming, Ganesh Sritheran Paneerselvam, Amal K Suleiman","doi":"10.1186/s12906-025-04816-y","DOIUrl":"10.1186/s12906-025-04816-y","url":null,"abstract":"<p><strong>Background: </strong>The use of traditional and complementary medicines (T&CM) among menopausal women has become prevalent due to the concerns on the adverse effects and differing opinions regarding hormone replacement therapy's (HRT) suitability for long-term following the release of Women's Health Initiative (WHI) results in 2002. To date, there have been no studies conducted on knowledge, attitude and practice towards T&CM among menopausal women in Malaysia. In addition, many women seek relief from menopausal symptoms by using T&CM despite questionable safety and efficacy. The objective of this study was to determine the knowledge, attitude and practice of menopausal women towards T&CM and its correlation with sociodemographic data in Kuala Lumpur and to assess the prevalence, types and perceived effectiveness of T&CM used by menopausal women in Kuala Lumpur.</p><p><strong>Methods: </strong>A cross-sectional study was conducted among menopausal women (n = 390) in Kuala Lumpur in the form of self-administered questionnaire. Data was collected using convenience sampling. The questionnaire was adopted from previous studies and some literature reviews with modifications. It was composed of 4 sections. Statistical analyses were performed using the IBM SPSS Statistics Version 26. Data obtained was analysed using descriptive and inferential analysis such as independent t test, one way ANOVA and chi square test.</p><p><strong>Results: </strong>The prevalence of T&CM used among menopausal women was found to be 27.2%. Evening primrose oil (53.2%), vitamins and minerals (53.2%) and black cohosh (48%) were most frequently used. Black cohosh and phytoestrogens were perceived as effective in relieving menopausal symptoms by 36.6% and 43.4% of respondents, respectively. Over half of the respondents had poor knowledge (51.3%), negative attitude (56.9%) and poor practice (55.7%) regarding the utilization of T&CM in menopause. There was a significant association between knowledge and attitude (p value < 0.05), as well as between attitude and practice (p value < 0.01). Participants with university level education and high income (> RM10,1000) showed higher knowledge and positive attitude.</p><p><strong>Conclusions: </strong>Menopausal women in Kuala Lumpur were found to have a low prevalence rate of T&CM utilization. Respondents' low understanding and unfavourable views might significantly impact the inadequate usage of T&CM.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"97"},"PeriodicalIF":3.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the effect of the Xianglian capsule on patients with diarrhea after cholecystectomy: a protocol for a single-center, randomized controlled trial.","authors":"Songmei Guan, Peiling Cai, De Cai, Shigang Duan","doi":"10.1186/s12906-025-04831-z","DOIUrl":"10.1186/s12906-025-04831-z","url":null,"abstract":"<p><strong>Introduction: </strong>Diarrhea is a common complication in patients after cholecystectomy. In China, tens of millions of patients experience diarrhea every year after cholecystectomy, which results in long-term pain in patients. Traditional Chinese medicine is a national treasure and has made great contributions to human health throughout the long history of the Chinese nation. However, as a classic ready-for-use traditional Chinese medicine, the exact clinical efficacy of the Xianglian preparation needs further observation. This article presents the protocol of a single-center, randomized controlled trial to evaluate the clinical efficacy of Xianglian capsules in patients with diarrhea after cholecystectomy.</p><p><strong>Methods and analysis: </strong>This study was conducted in the Department of Hepatobiliary and Pancreatic Surgery, Second Affiliated Hospital of Guangdong Medical University, following the recommendations of the current SPIRIT and CONSORT statements. We will recruit 90 patients who have developed diarrhea after cholecystectomy and randomize them 1:1 into the observation and control groups. The control group was given starch placebo capsules, and the observation group was given Xianglian capsules. Patient diarrhea-related indicators were collected at baseline and 5 days postdose. We will also collect patients' intestinal inflammation-related indicators and fecal microbial samples to analyze the possible mechanism of action of drugs.</p><p><strong>Discussion: </strong>This study will clarify the clinical effects of the Xianglian preparation on patients with diarrhea after cholecystectomy, provide evidence-based evidence, and promote the development and application of this classic prescription.</p><p><strong>Trial registration: </strong>http://www.chictr.org.cn .</p><p><strong>Trial number: </strong>ChiCTR2200061854. Registered on 04 July 2022.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"96"},"PeriodicalIF":3.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology mechanism of Rosmarinus officinalis L.(Rosemary) to improve cell viability and reduces apoptosis in treating Alzheimer's disease.","authors":"Jingzhi Zhao, Zhejian Li, Rongping Zhang, Haofei Yu, Lanchun Zhang","doi":"10.1186/s12906-025-04771-8","DOIUrl":"10.1186/s12906-025-04771-8","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neurodegeneration, nerve loss, neurofibrillary tangles and Aβ plaques. Different process of the AD pathology present more opportunities for treatment. In addition, the holistic approaches involving network pharmacology with traditional Chinese medicine (TCM) may be viable options for AD treatment, and lead to an effective cure for AD in the future. Therefore, this study explored the therapeutic effect and mechanism of Rosmarinus officinalis L(rosemary) in the treatment of Alzheimer's disease on basis of cell experiments, network pharmacology and molecular docking.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;We performed cell experiments, to investigate the therapeutic effects of Rosmarinus officinalis L on AD in vitro using CCK8 assay, flow cytometry and TMRE Kit. In addition, carnosic acid is a major antioxidant diterpenoid in Rosmarinus officinalis L. We performed cell experiments, to investigate the neuroprotective effects of carnosic acid on AD in virto using CCK8 assay and flow cytometry. Furthermore, the main antioxidant compounds of rosemary ware collected through literature reviews, PharmMapper and Swiss Target prediction ware used to identify their potential targets. Targets of AD were obtained from Genecards and OMIM. The intersection targets of the main active components of rosemary and the therapeutic targets of Alzheimer's disease were subsequently obtained by using online Venn diagram. Protein-protein interaction, Cytoscape, Gene Ontology, and Kyoto Encyclopedia of Genes were used to analyze potential targets and key pathways of rosemary in AD. Besides, through molecular docking, the interactions of the main active components of rosemary, and the predicted candidate targets were verified. Finally, quantitative Real-Time PCR (RT-qPCR) was performed to confirm the effectiveness of the genes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;It was found that rosemary could reversed Aβ25-35 induced damage to mouse hippocampal neuron HT22 cells, significantly improved the viability of damaged cells, and reduced apoptosis. The results of fluorescent staining with Hoechst 33,342 and TMRE suggested that rosemary inhibited the reduction of mitochondrial membrane potential levels induced by Aβ25-35, which had a specific protective effect on AD in vitro. Additionally, a main antioxidant compound in rosemary, carnosic acid, also has neuroprotective effects. Eight main antioxidant compounds of rosemary ware collected. Network pharmacology and molecular docking, revealed that rosemary plays a therapeutic role in the treatment of Alzheimer's disease through the main active carnosic acid, carnosol, rosmarinol, rosmadial, genkwanin, cirsimaritin, rosmarinic acid and caffeic acid in Rosmarinus officinalis L, which affects the gene regulation of HRAS, ESR1, RHOA, IGF1, SRC, ANXA5, MMP9, MAPK14, NOS3, and PIK3R1, and participates in the PI3K-Akt, Rap1, MAPK, and estrogen signa","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"94"},"PeriodicalIF":3.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonpharmaceutical treatment of distal sensorimotor polyneuropathy in diabetic patients: an unblinded randomized clinical trial.","authors":"Alexandra Strobel, Volha Laputsina, Viktoria Heinze, Susanne Schulz, Andreas Wienke, Marco Reer, Axel Schlitt","doi":"10.1186/s12906-025-04830-0","DOIUrl":"10.1186/s12906-025-04830-0","url":null,"abstract":"<p><strong>Background: </strong>For Diabetic polyneuropathy, the most prevalent form of polyneuropathy, there is a lack of evidence-based treatment options. Current approaches include pain management, alpha-lipoic acid, and antidepressants. Physical interventions, such as electrical stimulation (four-chamber galvanic bath) have been suggested but have limited supporting evidence. Heated granular stone therapy is another option to consider.</p><p><strong>Methods: </strong>An unblinded randomized controlled trials was conducted in 68 diabetic patients with distal sensorimotor polyneuropathy undergoing rehabilitation for diabetes mellitus as a primary or secondary diagnosis in the Paracelsus-Harz-Clinic (Quedlinburg, Germany). Patients were randomized into either the intervention group receiving heated granulated stone footbaths, or the control group receiving four-chamber galvanic baths. The primary endpoint was the assessment of any change in polyneuropathy using a vibration sensation test (Rydel-Seiffer scale, 8/8) from admission to discharge, analyzed by t-test and multivariable regression. Additionally, serum TNF-α and IL-6 as potential markers for polyneuropathy were compared over time using paired t-test.</p><p><strong>Results: </strong>The mean age of the patients was 66.8 ± 7.8 years; 63.2% were male and mean BMI was 32.2 ± 6.4 kg/m². Of the patients, 98.5% suffered from type 2 diabetes (one patient with type I diabetes); 82.4% were receiving oral antidiabetic medication; and 58.8% were insulin dependent. Distal sensorimotor polyneuropathy improved in both groups. The sum score increased from 16.7 to 22.6 in the study group and from 20.3 to 23.6 in the control group. A t-test showed a non-significant difference in the change of sum score between the treatment groups (2.6 points, p = 0.092), but adjusting for potential risk factors favors the intervention group (p = 0.043). Both analyzed markers decreased over time in each treatment group with IL-6 showing a clinical and significant reduction in the control group (p = 0.03).</p><p><strong>Conclusion: </strong>Diabetic patients with distal sensorimotor polyneuropathy benefit from physical treatment with administration of electrical stimulation (four-chamber galvanic bath) or a therapy with heated granulated stones three times a week. Our results indicate that heated stone therapy may be a potential treatment option. However, further research is required to understand the underlying biological processes.</p><p><strong>Trial registration: </strong>The study was registered in clinical trials.gov (identifier: NCT05622630, registration date: 18/11/2022).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"93"},"PeriodicalIF":3.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term effects of yoga-based practices on neural, cognitive, psychological, and physiological outcomes in adults: a scoping review and evidence map.","authors":"Gabriela Campelo, Jade Ramos de Araújo, Juan Pablo Aristizabal, Wânia de Souza, Goiara Mendonça de Castilho","doi":"10.1186/s12906-025-04825-x","DOIUrl":"10.1186/s12906-025-04825-x","url":null,"abstract":"<p><strong>Background: </strong>Compared with short-term practices, long-term yoga might promote differential qualitative and quantitative outcomes. Following JBI's and PRISMA-ScR guidelines, this scoping review followed an apriori and systematic protocol to document the long-term effects of yoga on neural, cognitive, psychological, and physiological outcomes, provide evidence maps for each yoga component, and summarize results identifying knowledge gaps and promising directions.</p><p><strong>Methods: </strong>Four databases (PubMed, Cochrane, LILACS, and PubPsych) were last searched in March 2023. Studies were included if they evaluated adults with > 1 year of practice, specified yoga-based practice(s) of interest, measured neural, cognitive, psychological, and/or physiological outcomes, were written in English, Spanish, or Portuguese, presented original data, and mentioned search terms in the title/abstract. Evidence maps for each yoga component followed a 4-level structure of outcome category, method, study design and literature size. Qualitative summaries followed the same structure, to facilitate information retrieval.</p><p><strong>Results: </strong>Out of 2270 screened studies, 65 were included (meditative studies = 36, predominantly postural studies = 25, breathing practice studies = 8). Physiologicaland neural outcomes were the most common, followed by psychological and cognitive outcomes. Although heterogeneous, neural results reveal structural and functional changes related to sensorimotor, interoceptive, emotion regulation, and executive functions. Physiologically, most studies have associated long-term practice with parasympathetic-driven autonomic, hormonal and immune responses, but some studies revealed sympathetic-driven or mixed responses, maybe due to the specific technique or individual differences. Psychological outcomes included lower levels of negative affect, psychopathological symptoms, and emotional reactivity. Cognitive measures have shown improved memory/cognition for older adults, but mixed or null effects for other constructs. Commonly, however, long-term practitioners demonstrated improved neural or physiological efficiency while performing cognitive tasks.</p><p><strong>Conclusions: </strong>Future research should provide clear descriptions of the investigated yoga practice, employ more experimental paradigms, and refine statistical reports and models. We encourage researchers to work with specific overarching theoretical frameworks to refine research predictions, such as the neurovisceral integration model or predictive coding models; to consider motivational, cultural, and contextual factors that might influence long-term outcomes; and to develop systematic reviews and meta-analyses as next steps of evidence summary.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"92"},"PeriodicalIF":3.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the therapeutic use of music to address anxiety for women undergoing gynaecological and fertility treatments.","authors":"Alison E Short, Natasha Andreadis, Geena Cheung, Virginia Stulz","doi":"10.1186/s12906-024-04739-0","DOIUrl":"10.1186/s12906-024-04739-0","url":null,"abstract":"<p><strong>Background: </strong>Music has been effectively used to address anxiety associated with medical treatments across broad applications. However, scant evidence exists about using music to reduce the significant anxiety experienced by women undergoing gynaecological procedures and fertility treatments. Such anxiety relates to the nature of procedures/examinations, invasiveness of the procedures, uncertainty around expectations, and intimate body part exposure, potentially affecting outcomes in triggering sympathetic nervous system responses. Music potentially contributes to anxiety management via known physiological and emotional effects. This funded collaborative project investigates therapeutic uses of music to address anxiety before and during gynaecology and fertility procedures, in order to assist participants with reducing their anxiety.</p><p><strong>Methods: </strong>Participants attending office, day surgery and other hospital procedures (N = 41) completed validated self-report surveys before and after procedures, listening to specific music via a purpose-designed Music Star. Additional contextual and qualitative data was sought to understand the nature of the experience for the women.</p><p><strong>Results: </strong>Results of this study indicated that the music intervention appeared to have a significant effect of reducing anxiety for women awaiting gynaecological and fertility procedures (p < .001, r = .82).</p><p><strong>Conclusions: </strong>The use of music forms an acceptable intervention to decrease anxiety in this context and can enhance the experience of women during treatment. Such an increased use of music can provide anxiety management benefits to women undergoing gynaecological and fertility treatments, with these experiences suggesting potential educational benefits to support women through this extremely stressful and complex stage of their life.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"91"},"PeriodicalIF":3.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium, magnesium, and vitamin D supplementations as complementary therapy for hypertensive patients: a systematic review and meta-analysis.","authors":"Samar A Amer, Dina Essam Abo-Elnour, Abdallah Abbas, Abdelrahman Salah Abdelrahman, Hossam-Eldin Mohamed Hamdy, Samar Kenawy, Menna M Sarhan, Omar Hany Mohamed, Mohamed Yousif Elnaghy, Mohammed Baker, Rawan Medhat El-Gayar, Omnia Samy El-Sayed, Moamen Mostafa Asla","doi":"10.1186/s12906-025-04809-x","DOIUrl":"10.1186/s12906-025-04809-x","url":null,"abstract":"<p><strong>Background: </strong>Hypertension, the first global modifiable risk factor for cardiovascular disease (CVD) morbidity and mortality, is a consequential and remediable threat to the health of individuals and society. Therefore, we conducted this study to explore the role of calcium (Ca⁺⁺), magnesium (Mg⁺⁺), and vitamin D (Vit-D) supplementation as complementary therapies for hypertension, focusing on their effects on systolic blood pressure (SBP), diastolic blood pressure (DBP), and pulse rate.</p><p><strong>Methods: </strong>This systematic review and meta-analysis examined relevant 6509 articles in PubMed, Scopus, Web of Science, and Cochrane CENTRAL up to October 2024. The primary outcome was the difference in blood pressure measurements (systolic and diastolic) and the pulse rate. The extracted data were analyzed using Open Meta Analyst software.</p><p><strong>Results: </strong>This systematic review and meta-analysis included 40 studies; of them, 24 studies were analyzed. Ca⁺⁺ was associated with a significant drop in the DBP (MD: -2.04, 95% CI [-3.39, -0.69], P = 0.01), but not in the SBP (P = 0.34) or pulse rate (P = 0.84). Mg⁺⁺ significantly reduced DBP (MD: -1.64, 95% CI [-3.19, -0.09], P = 0.04), but had no significant effect on the SBP (P = 0.16) or pulse rate (P = 0.81). The estimated effect of Vit-D showed a significant reduction in SBP (MD: -2.83, 95% CI [-5.47, -0.199], P = 0.04) and DBP (MD: -1.64, 95% CI [-2.97, -0.3], P = 0.01).</p><p><strong>Conclusion: </strong>Ca⁺⁺ and Mg⁺⁺ significantly reduced DBP but had no significant effect on SBP or the pulse rate. Whereas, vitamin D significantly reduced SBP and DBP.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"89"},"PeriodicalIF":3.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the essential oil extracts of Centaurea alexanderina different parts: GC-MS analysis, antimicrobial, antioxidant, and anti-hyperglycemic activities.","authors":"Hossam A Abdallah, Naglaa Afifi, Enas I A Mohamed, Mohamed Sebak, Rabab Mohammed, Mohamed A Zaki","doi":"10.1186/s12906-025-04805-1","DOIUrl":"10.1186/s12906-025-04805-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Natural products have been a cornerstone in the field of drug discovery for centuries, they have provided numerous therapeutic agents that have significantly impacted modern medicine. Centaurea alexanderina is a canescent herb that is native to Egypt and Libya and reported in Egyptian folk medicine as an anti-hyperglycemic, antioxidant, and antimicrobial herb. It is an understudied species, thusly, the target of this contribution was to perform a comparative study of the influence of plant part variation on its chemical and pharmacological characteristics. In this context, essential oil extracts from different parts of C. alexanderina (flowers, stems, leaves, and roots) have been analyzed chemically and tested for their antioxidant, antimicrobial, and anti-hyperglycemic activities.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The essential oil extracts from different parts of C. alexanderina have been prepared and their chemical compositions have been analyzed using GC-MS technique. Antimicrobial activities of different essential oil extracts were evaluated via the agar cup diffusion method. DPPH radical scavenging and FRAP assays were used for determination of the antioxidant capacity. Anti-hyperglycemic activities of the four essential oil extracts under study were investigated via α-glucosidase inhibition assay. A computational study has been performed using molecular docking of the identified compounds in the most active essential oil extracts (leaves and roots) against α-glucosidase enzyme using PDB entry 5NN8.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A GC-MS analysis of the four essential oil extracts revealed high levels of non-terpenoid hydrocarbons in three essential oil extracts (flower, stems, and leaves) and alcohols in roots essential oil extract, followed by fatty acids in flowers, diterpenoids in stems, steroids in leaves, and fatty acid esters in roots. Roots and stems essential oil extracts exhibited selective activity against Pseudomonas aeruginosa (P. aeruginosa), whereas leaves essential oil extract showed activity against both Salmonella enterica (S. enterica) and Escherichia coli (E. coli). Essential oil extracts of different parts of the plant exhibited DPPH free radical scavenging activity with IC&lt;sub&gt;50&lt;/sub&gt; values of 128, 134, 152, and 163 μM for flowers, roots, stems, and leaves essential oil extracts; respectively, while in FRAP assay, the results showed different pattern; flowers revealed the highest activity followed by leaves, roots, and stems (107.50 ± 3.64, 125.80 ± 4.2, 139.4 ± 5.27, and 155.59 ± 5.27 ug/mL, respectively). In vitro evaluation of anti-hyperglycemic activity via inhibition of α-glucosidase enzyme assay unveiled leaves and roots essential oil extracts as the most inhibitors with IC&lt;sub&gt;50&lt;/sub&gt; values of 349 and 369 μg/mL; respectively. The molecular docking study of detected metabolites in the most active essential oil extracts against human α-glucosidase enzyme (PDB: 5NN8) revealed mode","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"90"},"PeriodicalIF":3.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}