BMC Veterinary Research最新文献

{"title":"Integrative transcriptomic and proteomic analyses reveal that carbon metabolism and complement system of Madin Darby Bovine Kidney cells are affected by bovine coronavirus infection.","authors":"Hui Zhang, Qisheng Yu, Rui Guo, Qing Zhu, Jin Yu, Zhaohui Zhang, Lan Lan, Cheng Tang, Changqing Yu, Bin Zhang","doi":"10.1186/s12917-025-04848-z","DOIUrl":"10.1186/s12917-025-04848-z","url":null,"abstract":"<p><strong>Background: </strong>Bovine coronavirus (BCoV) is a major pathogen of bovine respiratory disease, causing respiratory and enteric infections in cattle and wild ruminants. It is responsible for economic losses and threatens the health and welfare of cattle industry.</p><p><strong>Results: </strong>In this study, a BCoV isolate, BCoV/SUWN/XHD-5, had cytopathogenic effects in Madin Darby bovine kidney (MDBK) cells and showed a high viral titer at 24 and 48 h post infection (hpi). Gene expression profiling using RNA sequencing and protein network mapping using the Tandem Mass Tag-based quantitative proteomics approach were performed in MDBK cells with BCoV infection at 24 and 48 hpi, respectively. Compared with mock-infected MDBK cells, 8,720 differentially expressed genes (DEGs) and 296 differentially expressed proteins (DEPs) were identified in BCoV infection at 24 hpi, whereas 5,838 DEGs and 747 DEPs were identified in BCoV infection at 48 hpi. Following GO annotation and KEGG enrichment analysis, most DEGs and DEPs were significantly enriched in metabolic pathways, endocytosis, ribosome and protein processing in endoplasmic reticulum, apoptosis and immune response. A correlation analysis of the proteome and transcriptome revealed that the up-regulated DEGs and DEPs were predominantly associated with metabolic pathways, apoptosis and the MAPK/TNF/Ras signaling pathway, whereas the down-regulated DEGs and DEPs were involved in complement and coagulation cascades and the Wnt signaling pathway. Importantly, BCoV decreases the mRNA and protein levels of complement component C3 in MDBK cells.</p><p><strong>Conclusions: </strong>The findings of this study provide the first report of the integrative transcriptomic and proteomic analyses of BCoV infection in MDBK cells. It revealed a regulatory network for analyzing the mechanisms of BCoV-host interactions and provides valuable insights into the pathogenesis of BCoV.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"398"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and factors associated with the infection of Babesia bigemina, Babesia bovis, and Theileria orientalis in Thale Noi Wetland buffaloes (Bubalus bubalis), Southern Thailand.","authors":"Chalutwan Sansamur, Kanpapat Boonchuay, Ruttayaporn Ngasaman, Kenny Oriel Aranas Olana, Veerasak Punyapornwithaya","doi":"10.1186/s12917-025-04846-1","DOIUrl":"10.1186/s12917-025-04846-1","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"397"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of foot and mouth disease in cattle in East Africa between 2014 and 2024: a systematic review and meta-analysis.","authors":"Gashaw Getaneh Dagnaw, Haileyesus Dejene","doi":"10.1186/s12917-025-04834-5","DOIUrl":"10.1186/s12917-025-04834-5","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"395"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-expression of LncRNA and mRNA in the cumulus-oocyte complex of rabbits exposed to ammonia.","authors":"Jia Cui, Fengyang Wu, Xinyu Yang, Yanhua Liu, Shudong Liu, Shuaijuan Han, Jiawei Guo, Baojiang Chen","doi":"10.1186/s12917-025-04806-9","DOIUrl":"10.1186/s12917-025-04806-9","url":null,"abstract":"<p><strong>Background: </strong>Ammonia (NH₃) is an environmental pollutant and a potent reproductive stressor widely found in rabbit houses. Exposure to ammonia can result in follicle atresia, affect oocyte maturation and cause cumulus cell apoptosis. Long non-coding RNA (lncRNA) is an important factor in the regulation of cumulus cell development and oocyte maturation. The potential molecular mechanism of NH₃ in the induction of cumulus-oocyte complex (COCs) toxicity and the regulatory role of lncRNA in COCs are currently unclear.</p><p><strong>Methods: </strong>A total of 150 female IRA rabbits (35 days old) were randomly divided into three groups, and kept in environmental control rooms for four weeks. The rabbits in the control group (CG) were kept under an NH₃ concentration of < 3 ppm. The two treatment groups were kept under NH₃ concentrations of 10 ppm (low ammonia concentration, LAC) and 30 ppm (high ammonia concentration, HAC). We used a combination of RNA deep sequencing, quantitative real-time polymerase chain reaction (qRT-PCR), and bioinformatic analysis to explore the regulatory mechanism of lncRNA and messenger RNA (mRNA) in COCs.</p><p><strong>Results: </strong>We found that primordial follicles and primary follicles were significantly decreased while atretic follicles were significantly increased in the NH₃-treated groups. The results from Gene Ontology (GO) items showed that female meiosis sister chromatid cohesion and the regulation of follicle-stimulating hormone secretion were involved in the mechanism of rabbits exposed to NH₃. The results demonstrated that the mammalian target of the rapamycin (mTOR) signaling pathway and the transforming growth factor-beta (TGF-beta) signaling pathway inhibits germ cell development and follicular growth in the LAC versus the CG group. LncRNAs were involved in the apoptosis of female germ cells via the hypoxia-inducible factor (HIF-1) signaling pathway in the HAC versus the CG group. Co-expression analysis found that lncRNA MAPK3 and lncRNA SHC1 were correlated with changes in cumulus cell and oocyte function after NH₃ exposure.</p><p><strong>Conclusions: </strong>These results indicate that NH₃ affected the development and function of COCs by influencing lncRNA expression.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"396"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species diversity, virulence, and antimicrobial resistance of the nasal staphylococcal and mammaliicoccal biota of reindeer.","authors":"Jolanta Kochel-Karakulska, Marta Maślanko, Marta Woroszyło, Małgorzata Szewczuk, Bartłomiej Grygorcewicz, Karol Fijałkowski","doi":"10.1186/s12917-025-04680-5","DOIUrl":"10.1186/s12917-025-04680-5","url":null,"abstract":"<p><strong>Background: </strong>Staphylococcus (S.) spp. and Mammaliicoccus (M.) spp., in addition to their established role as components of the human and animal microbiota, can also cause opportunistic infections. This study aimed to characterize bacteria recovered from nasal cavities of healthy adult reindeer from two farms located in Poland (15 reindeer) and Germany (15 reindeer). The research include bacteria isolation, species identification, detection of selected superantigen (SAg) genes, assessment of biofilm-forming capability in vitro, and evaluation of antimicrobial resistance.</p><p><strong>Results: </strong>Seventy-four staphylococci and mammaliicocci from 14 different species were isolated from 30 nasal swabs, with one to four strains obtained from each reindeer. The most frequently identified species was S. equorum, followed by S. succinus, M. sciuri, S. xylosus, M. lentus, S. chromogenes, S. devriesei, M. vitulinus, S. auricularis, S. agnetis, S. edaphicus, S. petrasii, S. simulans, and S. warneri. A greater species diversity was observed among the reindeer from Poland compared to those from Germany. All isolated bacteria were coagulase negative and clumping factor negative and did not carry any of the 21 analyzed SAg genes. M. sciuri demonstrated the highest antimicrobial resistance (100%), followed by S. succinus (91%) and S. equorum (78%). Resistance to rifampicin was the most common (30% strains). Sixteen strains (22%) exhibited biofilm production at least 10% greater than the strong biofilm-forming S. aureus ATCC 6538.</p><p><strong>Conclusions: </strong>This study reveals a significant knowledge gap regarding the nasal microbiota of reindeer. It contributes to our understanding of staphylococcal and mammaliicoccal biota of reindeer and underscores the necessity for monitoring of microbial populations to assess their health implications for both animals and humans, particularly concerning the zoonotic transmission of bacteria.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"394"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an indirect ELISA based on a new specific lipoprotein LP53 for the detection of antibodies against Mycoplasma synoviae.","authors":"Haoran Li, Zengjin Hu, Guijun Wang, Yu Wang, Shaohui Wang, Mingxing Tian, Yanqing Bao, Jingjing Qi, Shengqing Yu","doi":"10.1186/s12917-025-04827-4","DOIUrl":"10.1186/s12917-025-04827-4","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"393"},"PeriodicalIF":2.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on helminths detected in red foxes (Vulpes vulpes) in Bingol Province of Türkiye: morphological and molecular approaches.","authors":"Seyma Gunyakti Kilinc, Harun Kaya Kesik, Figen Celik, Sami Simsek","doi":"10.1186/s12917-025-04838-1","DOIUrl":"10.1186/s12917-025-04838-1","url":null,"abstract":"<p><strong>Background: </strong>Red foxes (Vulpes vulpes) are hosts to a large number of endoparasites, some of which are zoonotic. This study was carried out to identify and molecularly characterise intestinal helminths in foxes in Bingol province, eastern Türkiye. For this purpose, carcasses of 15 red foxes that died as a result of traffic accidents in Bingol province were obtained. Parasite material obtained from the intestines was identified according to its morphological characteristics, and complete gDNA was isolated from each individual parasite samples. A 446 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) gene was amplified using PCR. Subsequently, a unidirectional sequence analysis was conducted.</p><p><strong>Results: </strong>The mt-CO1 gene region of a total of 8 helminth species was successfully sequenced and identified by BLAST searches. One trematode (Alaria alata), five cestodes (Dipylidium caninum, Joyeuxiella sp., Taenia hydatigena, Mesocestoides litteratus, Mesocestoides sp.), one nematode (Toxascaris leonina) and one acanthocephalan (Macracanthorhynchus hirudinaceus) species were identified. Phylogenetic and haplotype analyses were performed on the obtained Mesocestoides spp. sequences. Haplotype analysis of Mesocestoides litteratus isolates revealed 22 haplotypes Mesocestoides.</p><p><strong>Conclusion: </strong>These findings are important to draw attention to the wild circulation of some zoonotic helminths.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"391"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes and practices of veterinarians and para-veterinarians towards antimicrobial stewardship in Malawi: underutilized strength in the fight against antimicrobial resistance.","authors":"Henson Kainga, Marvin Phonera, Simegnew Adugna Kallu, Elisha Chatanga, Merning Mwenifumbo, Joseph Nkhoma, Gilson Njunga","doi":"10.1186/s12917-025-04837-2","DOIUrl":"10.1186/s12917-025-04837-2","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) is a global public health concern, and the inappropriate use of antibiotics in animals and humans is considered a significant contributing factor. Para-veterinarians and veterinarians are vital in the fight against AMR. The study aimed to evaluate the knowledge, attitudes and practices of the para-veterinarians and veterinarians towards antimicrobial stewardship in Malawi. A cross-sectional questionnaire survey among the para-veterinarians and veterinarians was conducted from October 2024 to January 2025. A total of 69 participants, comprised of 42 para-veterinarians and 27 veterinarians, participated in this study, and the response rate for the survey was 44% (n = 158). The study used a predetermined cutoff of ≥ 55% to classify scores as having good knowledge, good practice, and a positive attitude. The study found that 85.5% of the respondents had good knowledge and 98.6% had good practices towards antimicrobial stewardship and prevention of AMR, while 92.8% had favorable attitude towards AMR and antimicrobial stewardship. The maximum obtainable scores for each construct were 11, 13 and 13 for knowledge, practice, and attitude, respectively. The overall mean scores for knowledge, practice and attitude were 11.25 ± 2.83, 8.71 ± 1.34 and 9.04 ± 1.64, respectively. These findings indicated that the respondents had good knowledge, good practices and favorable positive attitude towards AMR and antimicrobial stewardship. The study concludes that there is good level of knowledge, practice and favorable attitude among para-veterinarians and veterinarians towards antimicrobial stewardship which can be capitalized in the fight against AMR in Malawi. The veterinary professionals can be entrusted to comply with responsible antimicrobial prescriptions and use.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"390"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ligula intestinalis in Labeobarbus fish species at Lake Tana, Ethiopia: prevalence, histopathological investigation, and associated risk factors.","authors":"Tegegne Destaw, Mohammed Yessuf, Hailu Mazengia, Mengesha Ayehu Getnet, Asnakew Mulaw Berihun","doi":"10.1186/s12917-025-04799-5","DOIUrl":"10.1186/s12917-025-04799-5","url":null,"abstract":"<p><p>Food security and employment prospects are significantly influenced by the fish industry. A cross-sectional study was conducted from February 2023 to March 2024 to determine the pathological lesions, prevalence, and associated risk factors of L. intestinalis infection in Labeobarbus fish species in Lake Tana, Ethiopia. A total of 384 Labeobarbus fish were randomly selected from local fishermen and their associations. Pathological lesions were examined using proper evisceration and post-mortem techniques. Various visceral organs including the gonads, liver, and spleen were collected from infected fish for histopathological examination. Gross pathological findings revealed abdominal distension, anal hemorrhage, and gonadal atrophy, displacement of internal organs, body emaciation, and visceral fibrosis accompanied by discoloration. Histopathological alterations were observed in multiple organs. In the ovaries, the main changes included fibrosis, infiltration of inflammatory cells, destruction, and atrophy of oocytes. Testicular tissues exhibited hyperemia, necrosis, fibrosis, and degeneration. The liver showed vacuolar degeneration, biliary duct hyperplasia, infiltration of inflammatory cells, fibrosis, and necrosis. In the spleen, exudation, hemorrhage, congestion, and fibrosis were the primary pathological findings. Out of the 384 Labeobarbus fish examined, 62 tested positive for L. intestinalis, indicating an overall prevalence of 16.2% in Lake Tana. Statistical analysis revealed that fish standard length (χ² = 76.919, P = 0.001), fish weight (χ² = 50.087, P = 0.001), sampling points (χ² = 16.947, P = 0.009), and fish species (χ² = 27.73, P = 0.034) were significantly associated with L. intestinalis infection. The pathological effects of L. intestinalis on vital organs, combined with its high prevalence, suggest a significant negative impact on fish production and reproductive performance in Lake Tana. Therefore, regular monitoring and surveillance, public awareness campaigns, ecological studies, and appropriate policy interventions should be implemented to protect livelihoods and mitigate the effects of this parasite.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"392"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethiopian isolates of nematophagus fungi have effectively killed Haemonchus contortus infective larvae in vitro.","authors":"Tadesa Tilahun, Solomon Shiferaw, Hika Waktole, Gezahegne Mamo, Muluken Tekle, Getachew Terefe","doi":"10.1186/s12917-025-04844-3","DOIUrl":"10.1186/s12917-025-04844-3","url":null,"abstract":"<p><strong>Introduction: </strong>The effectiveness of anthelmintics in controlling gastrointestinal parasitism in livestock is increasingly threatened by the emergence of drug resistance. To address this issue, biological control methods have been proposed as potential alternatives. This research aimed to evaluate the efficacy of nematophagous fungi isolated from soil samples against Haemonchus contortus infective larvae.</p><p><strong>Material and methods: </strong>Soil samples were collected from Ambo and Hawasa, representing the central and southern regions of Ethiopia, respectively. The fungi were cultured on 2% water agar using the soil sprinkling technique. Fungal identification was conducted based on conidial morphology and adhesive networking.</p><p><strong>Results: </strong>Arthrobotrys oligospora (AO), Arthrobotrys conoides (AC) and Monacrosporium species (MS) were isolated from the soil samples. In vitro tests on 2% water agar demonstrated that the trapping and killing of H. contortus L₃ larvae began within 48 h of exposure. From second to 4th day of observation, significant difference in the number of dead larvae were observed between the untreated control (UTC) and treatment plates (P < 0.0001); mortality being 99.77 ± 0.38%, 96.74 ± 3.15%, 89.92 ± 4.31 and 9.92 ± 3.45% for AC, AO, MS and UTC groups. Monacrosporium species was less effective compared to both AC and AO (P > 0.05). When accounting for natural mortality (control), the contributions to larval deaths attributed to AC, AO, and MS were 90%, 89.7%, and 88.9%, respectively. The introduction of AC and AO spores to H. contortus egg-positive feces significantly decreased the number of live larvae recovered compared to the UTC (P < 0.0001) with percentage recovery of 1.6 ± 0.31%, 11.09 ± 1.41% and 49 ± 4.75% respectively. Sheep treated with conidia of AC and AO expelled varying numbers of conidia in the first 24 h post-treatment, with AO reducing larval development by 80-86% and AC by 84-87% when the natural reduction in the UTC plates is taken into account.</p><p><strong>Conclusion: </strong>This study for the first time has demonstrated the in vitro efficacy of nematode trapping fungal isolated from Ethiopian soil. Further research is needed to evaluate their performance on pasture.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":"21 1","pages":"388"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}