BMC Immunology最新文献

{"title":"Peripheral immune signatures associated with invasive behavior in pituitary neuroendocrine tumor: a blood-based analysis.","authors":"Hongyu Liu, Liangliang Wu, Chuanbiao Chen, Jialin Liu, Fangye Li, Ling Chen","doi":"10.1186/s12865-026-00840-w","DOIUrl":"10.1186/s12865-026-00840-w","url":null,"abstract":"<p><strong>Background: </strong>Pituitary neuroendocrine tumors (PitNETs) derived from adenohypophyseal cells are the third most frequent primary intracranial tumor. PitNETs that invades surrounding anatomical structures such as the cavernous sinus are defined as invasive PitNETs. Patients with invasive PitNETs often have co-morbidities and high recurrence rates. This study aimed to identify the cytokines associated with the invasive behavior of PitNETs and characterize the cytokine network and circulating immune cells in the peripheral immune microenvironment of invasive PitNETs.</p><p><strong>Methods: </strong>Peripheral blood samples were collected from 87 patients with PitNET before transsphenoidal surgery for the resection of PitNET. Knosp grade 3 A to 4 tumors on magnetic resonance imaging (MRI) were defined as invasive PitNETs. After strict screening according to the exclusion criteria, 40 patients with invasive PitNET and 41 with noninvasive PitNET were enrolled in this study. Blood samples from 20 patients with invasive PitNET and 21 patients with non-invasive PitNET were randomly selected for multiplex plasma cytokine analysis. In addition, 20 peripheral blood samples were collected from healthy volunteers as controls. Blood samples from the remaining 20 patients with invasive PitNET and 20 patients with non-invasive PitNET were used for peripheral blood cell subpopulation analysis.</p><p><strong>Results: </strong>The expression levels of MIF, MIP-1β, RANTES, IL-9, and TNF-β were increased in the peripheral blood of patients with invasive PitNET. Patients with invasive PitNET had higher levels of CD8⁺ T cells, CD8⁺ memory T cells, CD8⁺ Tregs, CD4⁺ T cells, CD4⁺ activated T cells, CD4⁺ memory T cells, Tregs, and macrophages, and lower levels of natural killer (NK) cells in the peripheral blood.</p><p><strong>Conclusion: </strong>MIF, MIP-1β, RANTES, IL-9, and TNF-β may serve as potential biomarkers for the diagnosis of invasive PitNET. The association between circulating immune cells and the cytokine network in the peripheral immune microenvironment is associated with the invasive behavior of PitNET.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13343741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced peripheral natural killer cell counts may be a predictive marker for cesarean scar pregnancy.","authors":"Ye Shen, Jinqiu Zhang, Hua Fang, Li Zhang, Qi Jiang, Hua Gong, Qi Chen, Min Zhao","doi":"10.1186/s12865-026-00841-9","DOIUrl":"10.1186/s12865-026-00841-9","url":null,"abstract":"<p><p>Cesarean scar pregnancy (CSP), a condition characterised by inflammation, occurs in 0.2% of women with a history of cesarean section(s), and is clinically categorised by type 1 and type 2 according to the ultrasound findings. Recent studies reported that systemic inflammation indices derived from routine whole-blood tests may serve as additional clinical predictive markers for early CSP detection, with lymphocyte counts showing a reduction. However, the specific lymphocyte populations contributing to this reduction remain unclear. This study included 72 women diagnosed with CSP and 50 gestation-matched controls. The subsets of peripheral lymphocytes, including CD45 lymphocytes/monocytes, CD3 T cells, CD4 T helper cells, CD8 cytotoxic T cells, CD19 B cells, total NK cells and NKT-like cells, were measured. We found a significant reduction in peripheral total NK cells and CD8 cytotoxic T cells in CSP women compared to controls. However, there were no differences in peripheral immune cell profiles between type 1 and type 2 CSP. Using total NK cell counts with a cut-off value of 210/µl, the area under the curve (AUC) was 0.698, with a sensitivity of 64%. Our findings suggest that the reduction in peripheral total NK cells and CD8 T cytotoxic cells may play a role in the development of CSP. Additionally, peripheral total NK cell counts, derived from routine whole-blood tests, could serve as a predictive marker for early CSP diagnosis.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13251172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Th1-Th2 balance in patients with recurrent implantation failure; a systematic review and meta-analysis.","authors":"Armin Derakhshanfar, Alireza Ebrahimi Iraei, Hosein Zarei Sharif, Zahra Yahoo, Maryam Hemmatzadeh, Saeed Aslani, Reza Kargar, Hamed Mohammadi","doi":"10.1186/s12865-026-00815-x","DOIUrl":"10.1186/s12865-026-00815-x","url":null,"abstract":"","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13359453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of bone marrow-derived cells in the treatment of experimental autoimmune type 1 diabetes: immunomodulatory and anti-apoptotic properties.","authors":"Mohamed Nassef, Amira Hafez, Soha Gomaa","doi":"10.1186/s12865-026-00823-x","DOIUrl":"10.1186/s12865-026-00823-x","url":null,"abstract":"<p><p>BACKGROUND: Bone marrow-derived cell (BMDC) transplantation is increasingly recognized as a valuable strategy in regenerative therapies. However, the data regarding the impact of BMDC transplantation on the severity of diabetes mellitus type 1 (DMT1). AIM: This study aimed to determine the effectiveness of adoptive transfer of BMDC isolated from either non-diabetic mice (nBMDC) or diabetic mice (dBMDC) in the treatment of a DMT1 mouse model. METHODS: Male Swiss albino mice underwent a 16-hour fasting period, followed by administration of streptozotocin (STZ) at a dose of 40 mg/kg body weight for five days to induce DMT1. After 14 days, diabetic mice were divided into four groups. The first group served as a diabetic control and received sodium citrate buffer, while the remaining three groups were treated for two weeks with one of the following: subcutaneous (s.c.) administration of insulin (8 U/kg/day), intravenous (i.v.) inoculation of nBMDCs (1 × 106 cells/mouse/once), or i.v. injections of dBMDC (1 × 106 cells/mouse/once). RESULTS: STZ-induced DMT1 mice treated with either nBMDC or dBMDC exhibited a noticeable increase in the expression of CD4+ T lymphocytes, CD8+ T lymphocytes, and NK lymphocytes, myeloid granulocytic neutrophil (CD11b+/Ly-6G+ cells), monocytic macrophage (CD11b+/Ly-6G− cells), and monocytic CD11b+/Ly-6G− cells. Additionally, there was a marked reduction in the percentages of CD25+ T cells, Foxp3+ cells, regulatory T cells (Tregs), CD25+/Foxp3+ cells, Treg CD25+/Foxp3− cells, Treg CD4+/CD25+ cells, and Treg CD4+/CD25− cells within the myeloid cell population in the spleen. The therapeutic efficacy of BMDC as an anti-diabetic therapy was further supported by a significant reduction in the proportion of early and late apoptosis in splenic leukocytes, accompanied by a slight increase in necrosis in STZ-induced DMT1 mice receiving either nBMDC or dBMDC. CONCLUSION: In summary, adoptive transfer of BMDCs exerts significant immunomodulatory and anti-apoptotic effects on pancreatic β cells, which are beneficial for pancreatic islets in DMT1 mice. Clinical studies are warranted to evaluate the safety and effectiveness of the adoptive transfer of BMDCs as a potential therapy for autoimmune DMT1.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the value and mechanism of miR-369-5p in childhood pneumonia.","authors":"Guoxia Luo, Congpo Deng, Bo Wen","doi":"10.1186/s12865-026-00833-9","DOIUrl":"10.1186/s12865-026-00833-9","url":null,"abstract":"<p><p>BACKGROUND: Childhood pneumonia (CP) represents a leading cause of pediatric mortality globally. Consequently, prompt diagnosis of CP is of great significance for its treatment and prognosis. OBJECTIVE: The objective of this study is to investigate the clinical significance of miR-369-5p in CP and its underlying mechanism. METHODS: This study enrolled clinical data from 69 CP patients and 60 healthy individuals. Through binary logistic analysis, ROC analysis, and Kaplan-Meier (KM) analysis, the diagnostic and prognostic utility of miR-369-5p for CP was determined. The RT-qPCR experiment was used to detect the expression levels of miR-369-5p, polymeric immunoglobulin receptor (PIGR), and inflammatory cytokines. The study used LPS (Lipopolysaccharide) to induce WI-38 cells to establish a pneumonia cell model for in vitro cell experiments and used the kit to detect the oxidative stress indicators. The cell proliferation and apoptosis were detected through CCK8 (Cell Counting Kit-8) and flow cytometry assays. The target genes of miR-369-5p were predicted using TargetScan database and subsequently validated via dual luciferase reporter assays. Pearson analysis revealed the correlation between miR-369-5p and PIGR. RESULTS: MiR-369-5p was markedly overexpressed in CP, serving as a diagnostic biomarker for CP, as well as being associated with a poor prognosis. LPS induction in WI-38 cells triggered miR-369-5p upregulation, which coincided with enhanced levels of inflammation, oxidative stress indicators and apoptosis, and a decline in proliferation capacity. PIGR was confirmed as a downstream target of miR-369-5p. Silencing miR-369-5p could lead to increased expression of PIGR, decreased expression of inflammatory factors and oxidative stress indicators, and enhanced cell activity. Meanwhile, inhibition of PIGR could weaken the effect of inhibiting miR-369-5p. CONCLUSION: Upregulated serum miR-369-5p serves as a promising non-invasive biomarker for the diagnosis and prognosis of CP. Inhibiting miR-369-5p can ameliorates the condition of CP by targeting PIGR.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147670336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal associations between Sjögren's syndrome and subtypes of endometrial cancer.","authors":"Yan Zhu, Qing Wu, Luyu Hu, Luanhong Wang","doi":"10.1186/s12865-026-00821-z","DOIUrl":"10.1186/s12865-026-00821-z","url":null,"abstract":"<p><p>BACKGROUND: Endometrial cancer (EC) is a heterogeneous gynecologic malignancy with distinct histological subtypes and diverse etiological factors. While autoimmune diseases have been associated with cancer development, the causal relationship between Sjögren’s syndrome (SS) and EC remains unclear. In this study, we employed a Mendelian randomization (MR) approach to investigate this complex association. METHODS: We conducted a two-sample MR study using genome-wide association study summary statistics from European ancestry cohorts. Genetic instruments for SS were derived from the FinnGen consortium, and outcome data for EC, including its endometrioid and non-endometrioid subtypes, were obtained from a large-scale EC meta-analysis. Multiple MR methods and sensitivity analyses were applied to assess robustness and detect pleiotropy. RESULTS: Genetically predicted SS was causally associated with an increased risk of overall EC (OR = 1.1480, 95% CI: 1.0511–1.2537, P = 0.0155), particularly endometrioid EC (OR = 1.1603, 95% CI: 1.0442–1.2894, P = 0.0207). No causal association was observed with non-endometrioid EC (OR = 1.0844, 95% CI: 0.8018–1.4665, P = 0.4560). Sensitivity analyses revealed no significant heterogeneity or horizontal pleiotropy, supporting the robustness of the findings. CONCLUSIONS: This MR study provides genetic evidence for a causal relationship between SS and increased risk of endometrioid EC, but not non-endometrioid EC. These findings suggest subtype-specific immune mechanisms underlying EC development and may inform targeted screening strategies for women with SS. Future research should explore the immuno-hormonal pathways linking autoimmunity and endometrial carcinogenesis.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the results of specific IgE detection for cat and dog dander allergens in 141, 165 tests with allergic diseases.","authors":"Yilin Li, Miaomiao Xu, Jing Li, Weihua Yang","doi":"10.1186/s12865-026-00825-9","DOIUrl":"10.1186/s12865-026-00825-9","url":null,"abstract":"&lt;p&gt;&lt;p&gt;OBJECTIVE: The incidence of pet allergies continues to rise. This study investigates the clinical distribution characteristics and changes of cat and dog dander allergens among all outpatients and inpatients treated at Hangzhou First People’s Hospital from 2016 to 2024. It aims to provide data support for clinical allergen screening, individualized avoidance strategies, and precision diagnosis and treatment. METHODS: A retrospective cohort study was conducted. All outpatients and inpatients who underwent serum allergen-specific IgE (sIgE) antibody testing at Hangzhou First People’s Hospital from January 2016 to December 2024 were retrospectively included. The study analyzed each test individually, repeated tests performed at different time points for the same patient were included in the analysis. The characteristics and trends of cat and dog dander allergens were analyzed over a nearly 9-year period, encompassing a total of 141,165 test instances. Male cases numbered 70,904 (50.23%), while female cases numbered 70,261 (49.77%). Patients were categorized by age group: 0–3 years (infant group, 6,729 cases, 4.76%), 4–12 years (child group), 13–18 years (adolescent group), 19–65 years (adult group), and &gt; 65 years (elderly group). This yielded 20,197 cases (14.31%) in the infant group, 55,280 cases (39.16%) in the child group, 6,729 cases (4.76%) in the adolescent group, 54,345 cases (38.50%) in the adult group, and 4,613 cases (3.27%) in the elderly group. Statistical analysis employed the χ² test. RESULTS: Among 141,165 test results, the sIgE positivity rate for cat dander allergen (3.76%) was higher than that for dog dander allergen (3.38%), with a statistically significant difference (χ²=11680.12, P &lt; 0.001). The positive rates for cat dander sIgE from 2016 to 2024 were 1.45%, 0.64%, 0.66%, 1.74%, 2.54%, 4.37%, 5.66%, 6.74%, and 9.36%, respectively. The positive rates for dog dander allergen sIgE sensitization from 2016 to 2024 were 0.57%, 0.16%, 0.09%, 0.25%, 0.50%, 3.30%, 5.77%, 7.09%, and 11.48%, respectively; Since 2018, the positive rates for cat and dog dander allergen sIgE have shown a significant upward trend over time in both males and females. The positive rates for cat and dog dander allergen sIgE were consistently higher in males than in females, with statistically significant differences (χ² = 11.922, χ² = 67.499, p &lt; 0.05). The positive rates for cat dander allergens (3.94%, 3.59%) were significantly higher than those for dog dander allergens (3.78%, 2.99%) in both males and females, with statistically significant differences (χ²=5121.29, χ²=6755.70, P &lt; 0.001); The positive rates for cat dander allergens in the infant/child group (2.30%) and elderly group (0.59%) were significantly lower than those for dog dander allergens (4.05%, 0.89%), with both differences being statistically significant (χ²=615.55, χ²=55.43, P &lt; 0.001); The positive rates for cat dander allergens in the children, adolescents, and adults groups (4.78%, 6","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13248293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of immunoglobulin A1/immunoglobulin A2 ratio and B cell lymphoma-6 in relation to apoptosis-associated speck-like protein in diabetic kidney disease patients.","authors":"Sara Youssry, Hossam Ghoneim, Yasser Ammar, Azza Magdy, Dalia Maharem","doi":"10.1186/s12865-026-00808-w","DOIUrl":"10.1186/s12865-026-00808-w","url":null,"abstract":"<p><strong>Background: </strong>Inflammation and alteration of the immune system play a key role in the pathogenesis of diabetes mellitus (DM). However, little is known about the underlying mechanisms involved in the inflammatory pathways of diabetic kidney disease (DKD). We aimed in this study to evaluate immunoglobulin A (IgA) subclasses/ratio and B cell lymphoma-6 (BCL-6) in relation to inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a caspase activation and recruiting domain) in diabetic patients with and without kidney disease. The present study was conducted on 50 diabetic patients who were classified into 25 patients without kidney disease and 25 DKD patients, together with 25 age-matched healthy individuals. Enzyme-linked immunosorbent assay (ELISA) was used to assess ASC and BCL-6 levels in culture supernatants of peripheral blood mononuclear cells as well as to determine IgA1 and IgA2 levels in individuals' serum.</p><p><strong>Results: </strong>The results revealed a significant increase in ASC levels together with a significant decrease in IgA1 and BCL-6 levels in DKD patients compared to other groups. A significant association was observed between the IgA1/ IgA2 ratio and improved disease markers in all patients. In addition, ASC levels were negatively correlated with both IgA2 and BCL-6 levels.</p><p><strong>Conclusions: </strong>It can be concluded that altered IgA subtypes and its ratio (IgA1/IgA2) as well as BCL-6 may be involved in the pathogenesis of DKD, suggesting their future therapeutic potential in DKD patients.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico design and immunoinformatics evaluation of a multi-epitope vaccine targeting SEPT9 for gastrointestinal adenocarcinomas.","authors":"Harsh Kashyap, Bhawna Rathi, Harkirat Singh, Navkiran Kaur, Shivani Sharda, Amarish Kumar Sharma","doi":"10.1186/s12865-026-00831-x","DOIUrl":"10.1186/s12865-026-00831-x","url":null,"abstract":"<p><p>BACKGROUND: Gastrointestinal adenocarcinomas are a major cause of mortality globally, underpinning the desire for new approaches to immunotherapeutic strategies. SEPT9 is clinically validated as an FDA-approved colorectal cancer biomarker, suggesting its potential as a candidate antigen for vaccine development. METHODS: An immunoinformatic pipeline created a multi-epitope chimera vaccination using strict standards of high antigenicity, non-allergenicity, non-toxicity, and worldwide population coverage. Five epitopes (three MHC-I and two MHC-II) were ranked from SEPT9. Selected epitopes were concatenated with suitable linkers for MHC-I and MHC-II, connected to a human β-defensin adjuvant. The construct composed of 134 amino acids was built using the trRosetta algorithm, refined using the GalaxyRefine2.0 algorithm, and validate via PROCHECK, ERRAT and ProSA. Immune response prediction was conducted by performing docking studies against TLR2 and TLR4 (via ClusPro software), and performing C-ImmSim immune simulations. RESULTS: The designed construct demonstrated predicted favorable physicochemical properties (solubility: 0.89), safety, and immunogenicity potential in silico. Docking ΔG values (TLR2: –17.7 kcal/mol; TLR4: –23.9 kcal/mol) and immune simulation data indicated theoretical potential to trigger innate and adaptive immunity. CONCLUSION: The SEPT9-based multi-epitope vaccine construct demonstrated promising predicted immunogenic and structural properties in silico. However, these findings are computational and hypothesis-generating; experimental validation is required to assess immunogenicity, safety, and therapeutic efficacy in biological systems.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of miR-548a-3p and its potential role in acute respiratory distress syndrome complicated with pulmonary fibrosis via OSM.","authors":"Siyu Lu, Feihong Huang, Qian Zhang, Xiulin Yang, Hongpeng Sun, Chunling Ji, Guangwen Long","doi":"10.1186/s12865-026-00820-0","DOIUrl":"10.1186/s12865-026-00820-0","url":null,"abstract":"<p><strong>Background: </strong>Acute respiratory distress syndrome (ARDS) is characterized by acute diffuse lung injury, with pulmonary fibrosis (PF) being a significant complication. The expression patterns and functional role of miR-548a-3p in ARDS and associated PF remain unexplored.</p><p><strong>Purpose: </strong>This study aims to delineate the diagnostic and prognostic significance of miR-548a-3p in ARDS and elucidate its underlying molecular mechanisms.</p><p><strong>Methods: </strong>Serum miR-548a-3p levels in ARDS patients were quantified using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Diagnostic value was evaluated via receiver operating characteristic (ROC) curves and binary logistic regression, and prognostic significance via Kaplan-Meier analysis and Cox regression. Interleukin-6 (IL-6)/Interleukin-8 (IL-8) levels were measured via enzyme-linked immunosorbent assay (ELISA). Potential targets were screened (NCBI/miRDB) and binding validated by dual-luciferase reporter assays. In lipopolysaccharide (LPS)-injured BEAS-2B cells, miR-548a-3p/Oncostatin M (OSM) regulation effects were examined via ELISA, Cell Counting kit-8 (CCK-8), and flow cytometry.</p><p><strong>Results: </strong>ARDS patients exhibited decreased miR-548a-3p expression, which correlated negatively with IL-6 and IL-8. Furthermore, miR-548a-3p effectively discriminated between ARDS patients and healthy individuals and served as a predictor of ARDS. In LPS-injured BEAS-2B cells, miR-548a-3p overexpression promoted proliferation, suppressed apoptosis, and reduced inflammation. OSM was identified as a direct target of miR-548a-3p through database screening and experimental validation. OSM overexpression reversed the protective effects of miR-548a-3p on LPS-injured lung epithelial cells.</p><p><strong>Conclusions: </strong>This study is the first to reveal that miR-548a-3p exerts protective effects in ARDS by targeting OSM, underscoring its great potential as a novel diagnostic and prognostic biomarker.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}