BMC Immunology最新文献

{"title":"Investigating the role of miR-192-5p as a diagnostic biomarker and the anti-inflammatory function in pediatric acute respiratory distress syndrome.","authors":"Qing Wang, Jianzhu He, Jing Li","doi":"10.1186/s12865-026-00829-5","DOIUrl":"10.1186/s12865-026-00829-5","url":null,"abstract":"<p><p>BACKGROUND: High mortality of pediatric acute respiratory distress syndrome (PARDS) demands new biomarkers. AIM: The study aims to clarify the clinical value of miR-192-5p in PARDS and its regulatory role in inflammatory responses. METHODS: Baseline data and serum samples were collected from healthy children, sepsis children, and sepsis-induced PARDS subjects. The receiver operating characteristic curve (ROC), Kaplan-Meier curve and multivariate Cox regression were used to assess miR-192-5p’s diagnostic and prognostic value for PARDS. The relationship between miR-192-5p and each index was evaluated by correlation analysis. The expression of miR-192-5p, inflammatory factors and ZEB2 in the subjects’ serum and human pulmonary microvascular endothelial cells (HPMECs) were detected by RT-qPCR. The cell viability and secretion of inflammatory factors were evaluated by CCK-8 and ELISA kit. Bioinformatic prediction combined with dual-luciferase reporter assay was used to verify the target genes. RESULTS: miR-192-5p was significantly low-expressed in PARDS patients’ serum. MiR-192-5p was a prognostic protective factor for PARDS, and low miR-192-5p indicated poor prognosis. MiR-192-5p was negatively correlated with TNF-α, IL-6, IL-1β, and APACHE II score. LPS treatment led to reduced HPMECs viability, excessive inflammatory factor production, and down-regulated miR-192-5p expression. Overexpression of miR-192-5p mimic could partially reverse the above phenomena, while knockdown could aggravate the above phenomena. ZEB2 was a downstream target gene of miR-192-5p. CONCLUSIONS: miR-192-5p serves as a diagnostic biomarker for PARDS and links to poor prognosis. Downregulation of miR-192-5p may promote LPS-induced inflammatory response and induce HPMECs cell viability by targeting ZEB2, thereby involving in the progression of PARDS.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteome-wide autoantibody screening and holistic autoantigenomic analysis unveil COVID-19 signature of autoantibody landscape.","authors":"Kazuki M Matsuda, Yoshiaki Kawase, Kazuhiro Iwadoh, Makoto Kurano, Yutaka Yatomi, Koh Okamoto, Kyoji Moriya, Hirohito Kotani, Teruyoshi Hisamoto, Ai Kuzumi, Takemichi Fukasawa, Asako Yoshizaki-Ogawa, Masanori Kono, Tomohisa Okamura, Hirofumi Shoda, Keishi Fujio, Kei Yamaguchi, Taishi Okumura, Chihiro Ono, Yuki Kobayashi, Ayaka Sato, Ayako Miya, Rikako Uchino, Yumi Murakami, Hiroshi Matsunaka, Hiroshi Imai, Shinichi Sato, Rudy Raymond, Ayumi Yoshizaki, Naoki Goshima","doi":"10.1186/s12865-026-00826-8","DOIUrl":"10.1186/s12865-026-00826-8","url":null,"abstract":"","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13321555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrophenol modulates the LRRK1/PIK3AP1 signaling axis: a novel strategy to mitigate inflammation and pyroptosis in gouty arthritis.","authors":"Xianjun Chen, Lin Shi, Qingjiang Pang, Rongyao Yu, Chenghao Wang","doi":"10.1186/s12865-026-00828-6","DOIUrl":"10.1186/s12865-026-00828-6","url":null,"abstract":"<p><strong>Background: </strong>Gouty arthritis (GA) is caused by hyperuricemia and the articular deposition of urate crystals, for which current treatments remain suboptimal. Considering the anti-inflammatory properties of Dendrobium extracts, especially Dendrophenol (Den), this study conducted an in-depth investigation into its effects on GA.</p><p><strong>Methods: </strong>Through methylthiazolyldiphenyl-tetrazolium bromide (MTT) test, the impact of Den on bone marrow-derived primary macrophages (BMDMs) was assessed to ascertain its non-toxic dosage range. The GA cell model was established, subjected to Den (0.5 µM) treatment, and transfected with leucine rich repeat kinase 1 (LRRK1) overexpression plasmid, after which the pyroptosis and inflammatory indices were detected. Bioinformatics was applied to examine GA-associated target genes and LRRK1 interaction targets. Cells with phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1) knockdown were generated to determine the mechanism by which Den improves GA.</p><p><strong>Results: </strong>0.5 µM Den significantly inhibited the expression of LRRK1 in GA cells, and down-regulated the pyroptosis level of cells and the contents of inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Overexpression of LRRK1 reversed these regulatory effects of Den. Bioinformatics analysis determined that PIK3AP1 was the downstream target of LRRK1. Den intervention reduced PIK3AP1 mRNA levels, and PIK3AP1 knockdown attenuated the effects of LRRK1 overexpression on pyroptosis and inflammatory factors.</p><p><strong>Conclusion: </strong>In our in vitro model, Den inhibits inflammation and pyroptosis by regulating the LRRK1/PIK3AP1 signaling pathway, highlighting this axis as a promising novel target for GA treatment.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13321610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Active tuberculosis disease in pregnancy alters frequencies of B-cell subsets among exposed neonates in Uganda.","authors":"Irene Andia Biraro, Diana Sitenda, Phillip Ssekamatte, Sande Matovu, Vilton Kasule, Rose Nakavuma, Marjorie Nakibuule, Andrew Peter Kyazze, Rose Nabatanzi, Davis Kibirige, Annettee Nakimuli, Felix Bongomin, Joseph Baruch Baluku, Stephen Cose","doi":"10.1186/s12865-026-00830-y","DOIUrl":"10.1186/s12865-026-00830-y","url":null,"abstract":"<p><p>BACKGROUND: Maternal tuberculosis (TB) remains a significant risk factor for compromising infant immune development in high-burden TB disease settings. The integrity of B-cells in early life is important for the development of immediate and long-term immunity in infants. This study assessed circulating B-cell subset frequencies in babies born to mothers with and without active TB in Uganda. METHODS: A cohort of 46 mother-infant pairs (24 cases, 22 controls) was recruited between September 2021 and July 2022 from three health facilities in Kampala. Infant cases were born to mothers with active TB, whereas infant controls were born to mothers without active TB. Peripheral blood mononuclear cells (PBMCs) were collected and processed from infants at baseline (≤ 1 month of age), and at three months and six months. PBMCs were stained with a 15-marker B-cell panel for spectral flow cytometry. Data were analysed using FlowJo and R v4.4.3. Mixed-effects modelling was applied for statistical testing, with time point as a fixed effect and participant as a random effect. Post-hoc comparisons used estimated marginal means. RESULTS: At baseline, infant cases were associated with decreased mean circulating frequencies of total B cells (18.5 versus 30.2; p = 0.02), naïve B cells (55.1 versus 68.9; p = 0.01), and mature B cells (43.6 versus 66.5; p = 0.03) compared to infant controls. Additionally, the infant cases were associated with increased baseline immature (56.4 versus 33.4; p = 0.03) and antibody-secreting B cells (15.6 versus 10.5; p = 0.04) compared to infant controls. No differences were observed at three or six months, except for IgG-only memory B cells, which were increased among infant cases at six months (14.4 versus 8.3; p = 0.04). CONCLUSIONS: Exposure to Mycobacterium tuberculosis in utero may decrease the preimmune B-cell repertoire in early life, potentially increasing the risk of neonatal infections and altered responses to routine infant vaccines. Prompt diagnosis and treatment of TB during pregnancy are therefore crucial to prevent poor neonatal morbidity and mortality in our setting.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterococcus faecalis KU-EF-004 enhances efficacy of anti-CTLA-4 immunotherapy for colorectal cancer via dendritic cell activation and gut microbiota modulation.","authors":"Taiki Yamazaki, Aori Minami, Koichi Kitagawa, Shihoko Yanase, Hideto Ueki, Toshiro Shirakawa","doi":"10.1186/s12865-026-00827-7","DOIUrl":"10.1186/s12865-026-00827-7","url":null,"abstract":"<p><p>BACKGROUND: Immune checkpoint inhibitors (ICI) have improved outcomes in mismatch repair–deficient/microsatellite instability–high colorectal cancer (CRC), yet only a subset of patients achieve durable benefit. The gut microbiota, particularly Enterococcus species, can modulate antitumor immunity and influence ICI efficacy. We investigated the probiotic potential of Enterococcus faecalis KU-EF-004, a non-antibiotic-resistant urinary isolate, as an adjunct to enhance ICI therapy in CRC. METHODS: MC38 tumor-bearing C57BL/6J mice received combination therapy with KU-EF-004 and anti-PD-1 or anti-CTLA-4 antibodies, and the therapeutic efficacy was compared with E. faecalis ATCC700802 strain and PBS-treated groups. Additionally, combination therapy with KU-EF-004 and either anti-PD-1 or anti-CTLA-4 antibodies was performed to evaluate which immune checkpoint inhibitor was associated with enhanced therapeutic effects. Dendritic cell activation in Peyer’s patches was assessed by flow cytometry, and gut microbiota changes were analyzed using 16S rRNA gene sequencing. RESULTS: KU-EF-004 markedly enhanced anti–CTLA-4 efficacy, reducing tumor growth and prolonging survival, whereas no benefit was observed with anti–PD-1. KU-EF-004 promoted dendritic cell activation and upregulated immune markers (CD80, CD86, IFN-γ, MHC II, IL-6, CD8a) in Peyer’s patches. 16S rRNA sequencing revealed increased microbial diversity and enrichment of Lactobacillus species following combined KU-EF-004 and anti–CTLA-4 treatment. CONCLUSION: These findings identify E. faecalis KU-EF-004 as a promising probiotic candidate to augment immune checkpoint blockade efficacy in colorectal cancer.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13104426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147467008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD30 knockout attenuates experimental colitis by reducing inflammatory cytokine production.","authors":"Lili Guo, Zhaocai He, Changqing Yang, Xiaorong Chen, Juan Wang","doi":"10.1186/s12865-026-00816-w","DOIUrl":"10.1186/s12865-026-00816-w","url":null,"abstract":"<p><p>BACKGROUND: Inflammatory bowel disease (IBD) is characterized by dysregulated T helper immune responses. Crohn’s disease is predominantly associated with excessive Th1 and Th17 responses, while ulcerative colitis displays a more heterogeneous immune profile. However, the molecular mechanisms underlying Th1 cell differentiation in intestinal inflammation remain incompletely understood. CD30, a member of the tumor necrosis factor receptor superfamily, has been implicated in various inflammatory conditions, yet its role in IBD pathogenesis has not been fully elucidated. OBJECTIVES: This study aimed to investigate the role of CD30 in experimental colitis and its regulation of Th1 cell differentiation through the NF-κB signaling pathway. METHODS: Acute colitis was induced in wild-type and CD30-deficient mice using 3% dextran sulfate sodium (DSS) for 7 days. CD30 expression in colonic tissues was assessed by immunohistochemistry, quantitative real-time PCR, and Western blot. Naive CD4 + T cells were isolated and stimulated under Th1-polarizing conditions with or without CD30 knockdown using small interfering RNA. Th1 differentiation was evaluated by flow cytometry with verification using T-bet and IL-17 A staining, and related transcription factors and cytokines were measured. The NF-κB signaling pathway was examined in lipopolysaccharide (LPS)-stimulated conditions. RESULTS: CD30 expression was significantly upregulated in DSS-induced colitis, correlating with increased Th1 markers including T-bet and IFN-γ. In vitro, CD30 knockdown markedly suppressed Th1 cell differentiation from naive CD4 + T cells, reducing IFN-γ + cell proportions among CD4 + T cells and downregulating T-bet, STAT4, and IL-12Rβ2 expression. Mechanistically, CD30 deficiency inhibited NF-κB pathway activation by reducing p65 phosphorylation and preventing IκBα degradation. In vivo, CD30-/- mice exhibited attenuated colitis severity with reduced disease activity index, improved colon length, decreased histological damage, and diminished Th1 cytokine production compared to wild-type littermates. CONCLUSIONS: CD30 promotes Th1 cell differentiation and exacerbates experimental colitis through NF-κB-dependent mechanisms. These findings identify CD30 as a potential therapeutic target for IBD treatment.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of immune cytokines and cell surface markers in FFPE biopsy samples from patients with suspected endometrial and lymph Node Tuberculosis.","authors":"Negash Baye, Abay Atnafu, Selfu Girma, Bethelehem Nigussie, Yerega Belete, Tigist Girma, Sofia Yimam, Kidist Bobosha, Zewiditu Chanyalew, Addisu Gize, Menberework Chanyalew","doi":"10.1186/s12865-026-00824-w","DOIUrl":"10.1186/s12865-026-00824-w","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Tuberculosis (TB), caused by the Mycobacterium tuberculosis complex (MTBC), remains a major global health concern. It manifests as pulmonary TB and extrapulmonary TB (EPTB), with the latter including TB lymphadenitis (TBLN) and endometrial TB (ETB). While TBLN is the most common form, ETB is among the least studied despite its serious clinical consequences, such as infertility. Owing to its asymptomatic nature, ETB is often diagnosed at a late stage after irreversible tissue damage has occurred. The immune response in TB varies by infection site, yet very little is known about the local immune response at the infection site of the ETB. Cytokines such as TNF-α, IFN-γ, and TGF-β play critical roles in TB immunity, but their expression patterns differ across infection sites. Limited data exist on site-specific cytokine responses in the ETB and TBLN, making it essential to investigate immune markers in these forms of TB. This study examines cytokine expression and T-cell surface markers in formalin-fixed, paraffin-embedded (FFPE) biopsy samples to provide insights into immune dynamics and potential biomarkers for EPTB diagnosis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A retrospective cross-sectional study was conducted using formalin-fixed, paraffin-embedded (FFPE) biopsy samples from ETB patients, TBLN patients, and controls. Immunohistochemistry (IHC) was performed to assess the expression of IFN-γ, TNF-α, and TGF-β, as well as CD4 + and CD8 + T-cell markers. Cytokine expression levels were quantified via QuPath softwares, and statistical comparisons were made via the Mann‒Whitney U test and the Kruskal‒Wallis test.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with controls, both ETB patients and TBLN patients presented significantly elevated IFN-γ and TNF-α expression (p &lt; 0.05). However, TGF-β expression was notably greater in the ETB than in the TBLN (p &lt; 0.05), suggesting a distinct immunoregulatory environment in the endometrium. CD4 + T-cell infiltration was significantly greater in the TBLN, whereas CD8 + T-cell presence was more pronounced in the ETB, indicating site-specific immune responses.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;This study highlights distinct cytokine expression patterns in patients with endometrial tuberculosis (ETB) and tuberculosis lymphadenitis (TBLN). The differential cytokine expression in the ETB may be influenced by the unique immune microenvironment of endometrial tissue. These site-specific immune responses could contribute to variations in disease presentation and progression. The differential expression of TGF-β in the ETB suggests a potential role in immune modulation and fibrosis, which may contribute to tissue remodeling and reproductive dysfunction. Additionally, the increased levels of IFN-γ and TNF-α in both EPTB forms reinforce the involvement of localized immune responses in disease pathogenesis. Identifying these cytokine patterns may help uncover potential biomarkers for early diagn","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic application regarding PILE score and the pan-immune-inflammation value in diffuse large B-Cell Lymphoma.","authors":"Chun Chang, Xiaoyong Man, Qi Hao, Wang Yan, Zhen Wang, Jingbo Wang","doi":"10.1186/s12865-026-00819-7","DOIUrl":"10.1186/s12865-026-00819-7","url":null,"abstract":"&lt;p&gt;&lt;p&gt;OBJECTIVE: This research explored the pan-immune-inflammation value (PIV) as prognostic significance factors in patients with diffuse large B-cell lymphoma (DLBCL). And we examined the PILE score, a composite parameter derived from lactate dehydrogenase (LDH), and Eastern Cooperative Oncology Group performance status (ECOG PS) and PIV as well. The findings were intended to contribute to more reliable prognostic assessment in clinical settings. METHODS: We retrospectively analyzed 90 patients diagnosed with DLBCL who accepted standard therapy consisting of a CD20 monoclonal antibody combined with the CHOP regimen (cyclophosphamide, doxorubicin, vincristine, and prednisone),with appropriate dose or cycle adjustments made according to age, cardiac function, and performance status.Specifically, CD20 monoclonal antibody was administered at 375 mg/m2 on day 0 of each 21-day cycle. CHOP consisted of cyclophosphamide 750 mg/m2, doxorubicin 50 mg/m2 (reduced to 30–40 mg/m2 in patients ≥70 years or with cardiac dysfunction), vincristine 1.4 mg/m2 on day 1, and prednisone 100 mg/m2 on days 1–5. Most patients received 6 cycles, while those with high-risk features received 8 cycles.A total of 16 patients with bulky disease, high-risk extranodal involvement, or suboptimal response to first-line chemotherapy as assessed by PET-CT received chemotherapy combined with radiotherapy.The PIV was established by integrating quantitative data on platelets, lymphocytes, monocytes and neutrophils obtained from routine peripheral blood tests. The PILE score was subsequently determined by integrating PIV with ECOG PS and LDH levels. Associations of PIV and PILE scores with treatment outcomes and prognosis were examined, and their potential relationships with pathological tumor characteristics were also evaluated. RESULTS: Elevated PIV was independently correlated with poorer overall survival (OS) (hazard ratio (HR) = 3.79; 95% confidence interval (CI): 1.31–10.97; p = 0.0135), PIV was statistically significant in univariate analysis for PFS (P&lt;0.001), making it the most robust prognostic marker among all assessed immune-inflammatory indices. Similarly, patients exhibiting higher PILE scores experienced significantly reduced progression-free survival (PFS) and OS (p &lt; 0.01, respectively). We evaluated the association of PIV, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), systemic immune-inflammation index (SII), and PILE with treatment outcomes by conducting both univariable Cox regression and multivariable logistic regression analyses, defining treatment response as the measured endpoint. While all markers showed significant association with the outcome in univariable analyses (p &lt; 0.01, respectively) at statistical level, none demonstrated independent predictive capacity in multivariable models. Among DLBCL patients, the PIV demonstrated an independent prognostic significance for OS outcomes. Furthermore,","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13067569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A combined model based on lactate clearance rate and SOFA score in prognostic assessment of patients with severe pneumonia.","authors":"Ying Chen, Chuankai Liu, Duandan Li, Jun Yan, Man Yi","doi":"10.1186/s12865-026-00817-9","DOIUrl":"10.1186/s12865-026-00817-9","url":null,"abstract":"<p><p>OBJECTIVE: To investigate the role of lactate clearance rate (LCR) and Sequential Organ Failure Assessment (SOFA) score in prognostic assessment of patients with severe pneumonia (SP). METHODS: A retrospective study of 185 SP patients was conducted. Baseline characteristics were compared between survivors (n = 128) and non-survivors (n = 57). Following univariate and correlation analyses, significant variables were used to build a combined prediction model via binary logistic regression. Model calibration and predictive performance for 28-day mortality were assessed using Hosmer-Lemeshow and ROC curve analyses, respectively. Dynamic changes in LCR and SOFA scores at admission (T0), 6 h (T6), and 24 h (T24) were compared. RESULTS: The 28-day mortality rate was 30.81% (57/185). Univariate analysis revealed statistically significant differences in age, chronic lung disease, procalcitonin (PCT), LCR, and SOFA score (P < 0.05). Spearman correlation analysis showed a moderate negative correlation between LCR and SOFA score (r = -0.401, P < 0.001). Multivariate analysis identified LCR [0.911 (0.861–0.965)] and SOFA score [1.508 (1.278–1.779)] as independent predictors (P < 0.05). The combined model demonstrated superior predictive accuracy (AUC: 0.882, 95% CI: 0.833–0.932) compared to LCR (AUC: 0.793) or SOFA score (AUC: 0.832) alone (Delong test, P < 0.05). Survivors exhibited significantly higher LCR and lower SOFA scores at T6 and T24, with improving trends over time, unlike non-survivors. CONCLUSION: The combined model integrating LCR and SOFA score outperforms either parameter alone in predicting 28-day mortality risk in SP, offering a potential tool for early risk stratification.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147343754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and optimization of in-house made indirect ELISA kit for the detection of antibodies against Pasteurella multocida in chicken.","authors":"Dubale Beyene, Anmaw Shite, Belayneh Getachew","doi":"10.1186/s12865-026-00818-8","DOIUrl":"10.1186/s12865-026-00818-8","url":null,"abstract":"<p><p>Fowl cholera is caused by P. multocida. The currently available commercial kit is relatively expensive for Ethiopian laboratories. To address this issue indirect ELISA kit to detect antibodies against P. multocida in chicken was developed. In-house made indirect ELISA kit was developed and optimized. Re-constituted local avian P. multocida biotype A was used for the preparation of coating antigen. The purity of the P. multocida biotype A inoculum was checked by gram staining and centrifugation at 4500 rpm for 30 min. The assay was developed and evaluated using 128 samples of chicken serum. Optimizations of various components were carried out against both anti-P. multocida hyper immune and pre immune serum. To develop an indirect ELISA test, washing buffer concentration of 500 µl of T-20, blocking buffer concentration of 200 µl, antigen concentration of 0.1 µg/ml, serum samples with a final dilution of 1:500 and optimal dilution of Rabbit anti-sheep IgG HRP-conjugate of 1: 1000 µg/ml are determined by checker board titration (CBT) method. The cut-off value was determined to be 0.23. Sensitivity, specificity and accuracy of in-house made indirect ELISA were 96%, 90% and 93%, respectively. This test result indicated good correlation with that of the commercial kit with a Cohen's κ agreement of 0.86 with a 95% confidence interval of 0.77 to 0.95. We found that in-house made indirect ELISA was more sensitive and convenient to perform. Consequently, in-house made indirect ELISA was as good as the commercial indirect ELISA in screening chicken serum samples for detection of antibodies against P. multocida.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13037160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}