Biomedical materials最新文献

A low-swelling alginate hydrogel with antibacterial hemostatic and radical scavenging properties for open wound healing. 用于开放性伤口愈合的具有抗菌止血和清除自由基特性的低肿胀藻酸盐水凝胶。

IF 4 3区医学

Biomedical materials Pub Date : 2024-09-10 DOI: 10.1088/1748-605x/ad792c

Xuebin Ma,Xiao Fu,Jianwen Meng,Hongmei Li,Fang Wang,Huarong Shao,Yang Liu,Fei Liu,Daizhou Zhang,Bo Chi,Jinlai Miao

{"title":"A low-swelling alginate hydrogel with antibacterial hemostatic and radical scavenging properties for open wound healing.","authors":"Xuebin Ma,Xiao Fu,Jianwen Meng,Hongmei Li,Fang Wang,Huarong Shao,Yang Liu,Fei Liu,Daizhou Zhang,Bo Chi,Jinlai Miao","doi":"10.1088/1748-605x/ad792c","DOIUrl":"https://doi.org/10.1088/1748-605x/ad792c","url":null,"abstract":"Development of a low-cost and biocompatible hydrogel dressing with antimicrobial, antioxidant, and low swelling properties is important for accelerating wound healing. Here, a multifunctional alginate hydrogel dressing was fabricated using the D-(+)-gluconic acid δ-lactone/CaCO3 system. The addition of hyaluronic acid and tannic acid (TA) provides the alginate hydrogel with anti-reactive oxygen species (ROS), hemostatic, and pro-wound healing properties. Notably, soaking the alginate hydrogel in a poly-ε-lysine (EPL) aqueous solution enables the alginate hydrogel to be di-crosslinked with EPL through electrostatic interactions, forming a dense network resembling \"armor\" on the surface. This simple one-step soaking strategy provides the alginate hydrogel with antibacterial and anti-swelling properties. Swelling tests demonstrated that the cross-sectional area of the fully swollen multifunctional alginate hydrogel was only 1.3 times its initial size, thus preventing excessive wound expansion caused by excessive swelling. After 5 hours of in vitro release, only 7% of TA was cumulatively released, indicating a distinctly slow-release behavior. Furthermore, as evidenced by the removal of 2,2-diphenyl-1-picrylhydrazyl free radicals, this integrated alginate hydrogel systems demonstrate a notable capacity to eliminate ROS. Full-thickness skin wound repair experiment and histological analysis of the healing site in mice demonstrate that the developed multifunctional alginate hydrogels have a prominent effect on extracellular matrix formation and promotion of wound closure. Overall, this study introduces a cost-effective and convenient multifunctional hydrogel dressing with high potential for clinical application in treating open wounds.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"1 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of properties for Carbothane™ 3575A-based electrospun vascular grafts in vitro and in vivo. 评估基于 Carbothane™ 3575A 的电纺血管移植物在体外和体内的特性。

IF 4 3区医学

Biomedical materials Pub Date : 2024-09-10 DOI: 10.1088/1748-605x/ad792d

Vera Sergeevna Chernonosova,Olesia Osipova,Zhou Nuankai,Inna Shundrina,Ivan S Murashov,Yurii Larichev,Andrey A Karpenko,Pavel P Laktionov

{"title":"Evaluation of properties for Carbothane™ 3575A-based electrospun vascular grafts in vitro and in vivo.","authors":"Vera Sergeevna Chernonosova,Olesia Osipova,Zhou Nuankai,Inna Shundrina,Ivan S Murashov,Yurii Larichev,Andrey A Karpenko,Pavel P Laktionov","doi":"10.1088/1748-605x/ad792d","DOIUrl":"https://doi.org/10.1088/1748-605x/ad792d","url":null,"abstract":"Bioengineered vascular grafts have emerged as a promising alternative to the treatment of damaged or occlusive vessels. It is thought that polyurethane-based scaffolds possess suitable hemocompatibility and biomechanics comparable to those of normal blood vessels. In this study, we investigated the properties of electrospun scaffolds comprising various blends of biostable polycarbonate-based polyurethane (Carbothane™ 3575A) and gelatin. Scaffolds were characterized by scanning electron microscopy, infra-red spectroscopy, small-angle X-ray scattering, stress-loading tests, and interactions with primary human cells and blood. Data from in vitro experiments demonstrated that a scaffold produced from a blend of 5% Carbothane™ 3575A and 10% gelatin has proven to be a suitable material for fabricating a small-diameter vascular graft. A comparative in vivo study of such vascular grafts and ePTFE grafts implanted in the abdominal aorta of Wistar rats was performed. The data of intravital study and histological examination indicated that Carbothane-based electrospun grafts outclass ePTFE grafts and represent a promising device for preclinical studies to satisfy vascular surgery needs.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"13 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Migration and retention of human osteosarcoma cells in bioceramic graft with open channel architecture designed for bone tissue engineering. 人骨肉瘤细胞在为骨组织工程设计的具有开放通道结构的生物陶瓷移植物中的迁移和存留。

IF 4 3区医学

Biomedical materials Pub Date : 2024-09-10 DOI: 10.1088/1748-605x/ad792b

Gayathry G,Francis Boniface Fernandez,Harikrishna Varma,Manoj Komath

{"title":"Migration and retention of human osteosarcoma cells in bioceramic graft with open channel architecture designed for bone tissue engineering.","authors":"Gayathry G,Francis Boniface Fernandez,Harikrishna Varma,Manoj Komath","doi":"10.1088/1748-605x/ad792b","DOIUrl":"https://doi.org/10.1088/1748-605x/ad792b","url":null,"abstract":"The microstructure of a porous bioceramic bone graft, especially the pore architecture, plays a crucial role in the performance of the graft. Conventional bioceramic grafts typically feature a random, closed-pore structure, limiting biological activity to the periphery of the graft. This can lead to delay in full integration with the host site. Bioceramic forms with open through pores can perform better because their inner regions are accessible for natural bone remodelling. This study explores the influence of open through pores in a bioceramic graft on the migration and retention of the local cells in vitro, which will correlate to the rate of healing in vivo. Hydroxyapatite (HA) ceramic forms with aligned channels were fabricated using slip casting technique, employing sacrificial fibers. The sorption characteristics across the graft were evaluated using HOS cell line. Seven-day cultures showed viable cells within the channels, confirmed by live/dead assay, SEM analysis, and cytoskeletal staining, indicating successful cell colonization. The channel architecture effectively enhances cell migration and retention throughout its entire structure, suggesting potential applications in bone tissue engineering based on the results obtained.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"6 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of induction heating capability of bioactive SiO2–CaO–Na2O–P2O5 glass-ceramics by selective substitution with magnetite nanoparticles 通过选择性替代磁铁矿纳米颗粒提高生物活性 SiO2-CaO-Na2O-P2O5 玻璃陶瓷的感应加热能力

IF 4 3区医学

Biomedical materials Pub Date : 2024-06-20 DOI: 10.1088/1748-605x/ad51c0

Nitu, Rushikesh Fopase, Lalit Mohan Pandey, Jyoti Prasad Borah and Ananthakrishnan Srinivasan

引用次数: 0

Antiproliferative efficacy and mechanism of action of garlic phytochemicals-functionalized gold nanoparticles in triple-negative breast cancer cells 大蒜植物化学物质功能化金纳米粒子在三阴性乳腺癌细胞中的抗增殖功效和作用机制

IF 4 3区医学

Biomedical materials Pub Date : 2024-04-28 DOI: 10.1088/1748-605x/ad3ff9

Kimaya Meher, Harshad Paithankar, Ramakrishna V Hosur and Manu Lopus

{"title":"Antiproliferative efficacy and mechanism of action of garlic phytochemicals-functionalized gold nanoparticles in triple-negative breast cancer cells","authors":"Kimaya Meher, Harshad Paithankar, Ramakrishna V Hosur and Manu Lopus","doi":"10.1088/1748-605x/ad3ff9","DOIUrl":"https://doi.org/10.1088/1748-605x/ad3ff9","url":null,"abstract":"Fabrication of gold nanoparticles (GNPs) with phytochemicals is an emerging green nanotechnology approach with therapeutic implications. Garlic, known for its culinary and medicinal properties, has been extensively investigated for its anticancer properties. Here, we report a method to substantially enhance the antiproliferative potency of garlic by functionalizing its phytochemicals to GNPs and demonstrate a possible mechanism of action of these nanoparticles in the triple-negative breast cancer cell line, MDA-MB-231. Garlic gold nanoparticles (As-GNPs) were synthesized using garlic extract (As-EX) and gold chloride and characterized using a variety of spectroscopy techniques, and transmission electron microscopy (TEM). Compared to As-EX, which has a negligible effect on the viability of the cells, As-GNPs inhibited cell viability with an IC50 of 0.310 ± 0.04 mg ml−1 and strongly inhibited the clonogenic and migratory propensities of these cells. As indicated by TEM, the As-GNPs entered the cells via endocytosis and dispersed in the cellular milieu. Since tubulin, the protein involved in cell division, is a verified target for several antiproliferative drugs, we next examined whether the As-GNPs interact with this protein. The As-GNPs showed concentration-dependent binding to purified tubulin, slightly but consistently perturbing its secondary helical integritywithout grossly damaging the tertiary structure of the protein or the net polymer mass of the microtubules, as indicated by a tryptophan-quenching assay, far UV-circular dichroism spectroscopy, anilinonaphthalene sulfonate-binding assay, and polymer mass analysis, respectively. In cells, As-GNPs killed the cancer cells without cell cycle arrest, as evidenced by flow cytometry.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"22 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140812187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of chitosan/polyvinyl alcohol antibacterial sponge materials 壳聚糖/聚乙烯醇抗菌海绵材料的制备与表征

IF 4 3区医学

Biomedical materials Pub Date : 2024-04-17 DOI: 10.1088/1748-605x/ad3c87

Xue Chen, Jing Xu, Alidha Gafur, Baoyu Chen, Yibo Han, Liyuan Zhang, Lingwen Kong, Guixue Wang and Zhiyi Ye

引用次数: 0

Surface engineered nanodiamonds: mechanistic intervention in biomedical applications for diagnosis and treatment of cancer 表面工程纳米金刚石：癌症诊断和治疗生物医学应用中的机理干预

IF 4 3区医学

Biomedical materials Pub Date : 2024-04-15 DOI: 10.1088/1748-605x/ad3abb

Tanima Dey, Anushikha Ghosh, Arka Sanyal, Chelsea Josephine Charles, Sahas Pokharel, Lakshmi Nair, Manjari Singh, Santanu Kaity, Velayutham Ravichandiran, Kulwinder Kaur, Subhadeep Roy

{"title":"Surface engineered nanodiamonds: mechanistic intervention in biomedical applications for diagnosis and treatment of cancer","authors":"Tanima Dey, Anushikha Ghosh, Arka Sanyal, Chelsea Josephine Charles, Sahas Pokharel, Lakshmi Nair, Manjari Singh, Santanu Kaity, Velayutham Ravichandiran, Kulwinder Kaur, Subhadeep Roy","doi":"10.1088/1748-605x/ad3abb","DOIUrl":"https://doi.org/10.1088/1748-605x/ad3abb","url":null,"abstract":"In terms of biomedical tools, nanodiamonds (ND) are a more recent innovation. Their size typically ranges between 4 to 100 nm. ND are produced via a variety of methods and are known for their physical toughness, durability, and chemical stability. Studies have revealed that surface modifications and functionalization have a significant influence on the optical and electrical properties of the nanomaterial. Consequently, surface functional groups of NDs have applications in a variety of domains, including drug administration, gene delivery, immunotherapy for cancer treatment, and bio-imaging to diagnose cancer. Additionally, their biocompatibility is a critical requisite for their <italic toggle=\"yes\">in vivo</italic> and <italic toggle=\"yes\">in vitro</italic> interventions. This review delves into these aspects and focuses on the recent advances in surface modification strategies of NDs for various biomedical applications surrounding cancer diagnosis and treatment. Furthermore, the prognosis of its clinical translation has also been discussed.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"42 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140608818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of novel bioadhesive chitosan film loaded with bimetallic gold-silver nanoparticles for antibiofilm and wound healing activity 负载双金属金-银纳米粒子的新型生物粘附壳聚糖膜的设计及其抗生物膜和伤口愈合活性

IF 4 3区医学

Biomedical materials Pub Date : 2023-02-02 DOI: 10.1088/1748-605X/acb89b

C. Singh, A. K. Mehata, V. ., P. Tiwari, Aseem Setia, Ankit Malik, Sanjeev K Singh, Rashmi M. Tilak, M. S. Muthu

{"title":"Design of novel bioadhesive chitosan film loaded with bimetallic gold-silver nanoparticles for antibiofilm and wound healing activity","authors":"C. Singh, A. K. Mehata, V. ., P. Tiwari, Aseem Setia, Ankit Malik, Sanjeev K Singh, Rashmi M. Tilak, M. S. Muthu","doi":"10.1088/1748-605X/acb89b","DOIUrl":"https://doi.org/10.1088/1748-605X/acb89b","url":null,"abstract":"Microbial infections and antibiotic resistance are among the leading causes of morbidity and mortality worldwide. The bimetallic chitosan (CS)-capped gold-silver nanoparticles (CS-AuAg-NPs) were prepared by the seeded growth synthesis technique. The nanoparticles were optimized for particle size (PS), zeta potential (ZP) and antibacterial activity by Box–Behnken design at three levels and three factors. The developed CS-AuAg-NPs were polydispersed with mean hydrodynamic PS in the range of 55 – 289 nm and ZP ranges from +8.53 mV to +38.6 mV. The optimized CS-AuAg-NPs found to have a minimum inhibitory concentration and minimal bactericidal concentration of 1.625 ± 0.68 and 3.25 ± 0.74 µg ml−1 towards multidrug resistant (MDR) Staphylococcus aureus ATCC 25923 (MDR AT) and 3.25 ± 0.93 and 3.25 ± 0.86 µg ml−1 towards MDR S. aureus clinical isolate MDR1695 (MDR CI) strain, respectively. The CS-AuAg-NPs were much more effective against MDR AT and MDR CI compared to clindamycin standard. The live/dead assay of clinical isolates strain demonstrated significant reduction of bacterial cells ∼67.52 folds compared to control group in 12 h. The hemolysis study suggested that CS-AuAg-NPs were non-hemolytic and safer for application in the wound. Furthermore, CS-AuAg-NPs were distributed in the CS film, which showed 87% wound recovery after 7 d in mice model. Hence, we concluded that CS-AuAg-NPs was safer and more effective against MDR bacteria and capable of skin regeneration in the infected wound.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":"18 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42351654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

3D printed bioresorbable scaffolds for articular cartilage tissue engineering: a comparative study between neat polycaprolactone (PCL) and poly(lactide-b-ethylene glycol) (PLA-PEG) block copolymer 3D打印关节软骨组织工程生物可吸收支架:纯聚己内酯(PCL)与聚乳酸-b-乙二醇(PLA-PEG)嵌段共聚物的比较研究

IF 4 3区医学

Biomedical materials Pub Date : 2022-06-14 DOI: 10.1088/1748-605X/ac78b7

Uzuri Urtaza, O. Guaresti, Izar Gorroñogoitia, Ana Zubiarrain-Laserna, Emma Muiños‐López, Froilán Granero-Moltó, JM Lamo de Espinosa, T. López-Martínez, M. Mazo, F. Prósper, A. Zaldua, J. Anakabe

引用次数: 2

A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing 用于添加剂组织制造的可调明胶-透明质酸双醛/甲基丙烯酰明胶互穿聚合物网络水凝胶

IF 4 3区医学

Biomedical materials Pub Date : 2022-06-14 DOI: 10.1088/1748-605X/ac78b8

R. Anand, Mehdi Salar Amoli, An-Sofie Huysecom, P. Amorim, Hannah Agten, L. Geris, V. Bloemen

{"title":"A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing","authors":"R. Anand, Mehdi Salar Amoli, An-Sofie Huysecom, P. Amorim, Hannah Agten, L. Geris, V. Bloemen","doi":"10.1088/1748-605X/ac78b8","DOIUrl":"https://doi.org/10.1088/1748-605X/ac78b8","url":null,"abstract":"Methacryloyl gelatin (GelMA) is a versatile material for bioprinting because of its tunable physical properties and inherent bioactivity. Bioprinting of GelMA is often met with challenges such as lower viscosity of GelMA inks due to higher methacryloyl substitution and longer physical gelation time at room temperature. In this study, a tunable interpenetrating polymer network (IPN) hydrogel was prepared from gelatin-hyaluronan dialdehyde (Gel-HDA) Schiff’s polymer, and 100% methacrylamide substituted GelMA for biofabrication through extrusion based bioprinting. Temperature sweep rheology measurements show a higher sol-gel transition temperature for IPN (30 °C) compared to gold standard GelMA (27 °C). Furthermore, to determine the tunability of the IPN hydrogel, several IPN samples were prepared by combining different ratios of Gel-HDA and GelMA achieving a compressive modulus ranging from 20.6 ± 2.48 KPa to 116.7 ± 14.80 KPa. Our results showed that the mechanical properties and printability at room temperature could be tuned by adjusting the ratios of GelMA and Gel-HDA. To evaluate cell response to the material, MC3T3-E1 mouse pre-osteoblast cells were embedded in hydrogels and 3D-printed, demonstrating excellent cell viability and proliferation after 10 d of 3D in vitro culture, making the IPN an interesting bioink for the fabrication of 3D constructs for tissue engineering applications.","PeriodicalId":9016,"journal":{"name":"Biomedical materials","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42965941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4