A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
R. Anand, Mehdi Salar Amoli, An-Sofie Huysecom, P. Amorim, Hannah Agten, L. Geris, V. Bloemen
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引用次数: 4

Abstract

Methacryloyl gelatin (GelMA) is a versatile material for bioprinting because of its tunable physical properties and inherent bioactivity. Bioprinting of GelMA is often met with challenges such as lower viscosity of GelMA inks due to higher methacryloyl substitution and longer physical gelation time at room temperature. In this study, a tunable interpenetrating polymer network (IPN) hydrogel was prepared from gelatin-hyaluronan dialdehyde (Gel-HDA) Schiff’s polymer, and 100% methacrylamide substituted GelMA for biofabrication through extrusion based bioprinting. Temperature sweep rheology measurements show a higher sol-gel transition temperature for IPN (30 °C) compared to gold standard GelMA (27 °C). Furthermore, to determine the tunability of the IPN hydrogel, several IPN samples were prepared by combining different ratios of Gel-HDA and GelMA achieving a compressive modulus ranging from 20.6 ± 2.48 KPa to 116.7 ± 14.80 KPa. Our results showed that the mechanical properties and printability at room temperature could be tuned by adjusting the ratios of GelMA and Gel-HDA. To evaluate cell response to the material, MC3T3-E1 mouse pre-osteoblast cells were embedded in hydrogels and 3D-printed, demonstrating excellent cell viability and proliferation after 10 d of 3D in vitro culture, making the IPN an interesting bioink for the fabrication of 3D constructs for tissue engineering applications.
用于添加剂组织制造的可调明胶-透明质酸双醛/甲基丙烯酰明胶互穿聚合物网络水凝胶
甲基丙烯酰明胶(GelMA)具有可调的物理性能和固有的生物活性,是一种多用途的生物打印材料。GelMA的生物打印经常遇到挑战,例如由于较高的甲基丙烯酰基取代度和室温下较长的物理凝胶化时间,GelMA油墨的粘度较低。在本研究中,以明胶-透明质酸二醛(Gel HDA)Schiff’s聚合物和100%甲基丙烯酰胺取代的GelMA为原料,通过挤出生物打印制备了可调互穿聚合物网络(IPN)水凝胶。温度扫描流变学测量显示,与金标准GelMA(27°C)相比,IPN(30°C)的溶胶-凝胶转变温度更高。此外,为了确定IPN水凝胶的可调性,通过组合不同比例的Gel HDA和GelMA制备了几个IPN样品,获得了20.6±2.48 KPa至116.7±14.80 KPa的压缩模量。我们的结果表明,可以通过调节GelMA和Gel-HDA的比例来调节室温下的机械性能和可印刷性。为了评估细胞对该材料的反应,将MC3T3-E1小鼠前成骨细胞包埋在水凝胶中并进行3D打印,在3D体外培养10天后显示出优异的细胞活力和增殖,使IPN成为制造组织工程应用的3D构建体的有趣的生物墨水。
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来源期刊
Biomedical materials
Biomedical materials 工程技术-材料科学:生物材料
CiteScore
6.70
自引率
7.50%
发文量
294
审稿时长
3 months
期刊介绍: The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters
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