{"title":"Sodium Benzoate, Potassium Sorbate, and Citric Acid Food Preservatives Trigger Apoptosis in the Male Reproductive System of Rats.","authors":"Marziyeh Haghshenas, Seyyed Sajad Daneshi, Ahmadi Hassan Nategh, Samaneh Bina, Fateme Esmaeilpoor, Razieh Bagheri, Mohammad Javad Khoshnoud, Azad Salimi, Seyedeh Leili Asadi-Yousefabad, Marzieh Rashedinia","doi":"10.1155/bmri/8837003","DOIUrl":"10.1155/bmri/8837003","url":null,"abstract":"<p><p>The safety of food additives has received significant attention. While individual additives used within specified limits are considered safe, examining the effects of food additives is essential, especially when they are ingested in combination or when consumption exceeds acceptable daily intake levels. In this study, the effects of three common food preservatives-sodium benzoate (SB), potassium sorbate (PS), and citric (Cit) acid-on biochemical markers and histopathology of the male reproductive system were evaluated. Thirty-six male Sprague-Dawley rats received the no observed adverse effect level (NOAEL) doses of SB, PS, and Cit alone or in combination in their drinking water for 8 weeks. Blood samples and the epididymis and testes were surgically collected for analysis. Results showed significant decreases in testicular weight, reduced sperm count and viability, and increased immotile sperm in the SB, PS, and SB + PS groups. These changes were associated with notable decreases in plasma testosterone levels in all treated groups. Elevated lipid peroxidation levels, increased Bax/Bcl-2 ratios, and caspase-3 activity in the combined groups demonstrated induction of apoptosis in testes. Additionally, histopathological analysis revealed degenerative changes in testicular structure and disruption of spermatogenesis. In conclusion, the combined use of these preservatives may lead to reproductive issues in rats, raising concerns about their potential effects on human health.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"8837003"},"PeriodicalIF":2.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Richard Okyere Boadu, Victor Wireko Adu, Kwame Adu Okyere Boadu, Godwin Adzakpah, Nathan Kumasenu Mensah, Emmanuella Esi Arhin, Augustine Ilinkakor Nisanman, Godwin Salakpi, Stephen Ekow Bessabro, Williams Danquah, Idris Adam Simsiah, Emmanuel Obour
{"title":"Competency Level in Generation and Usage of Health Information Within the Landscape of Ghana.","authors":"Richard Okyere Boadu, Victor Wireko Adu, Kwame Adu Okyere Boadu, Godwin Adzakpah, Nathan Kumasenu Mensah, Emmanuella Esi Arhin, Augustine Ilinkakor Nisanman, Godwin Salakpi, Stephen Ekow Bessabro, Williams Danquah, Idris Adam Simsiah, Emmanuel Obour","doi":"10.1155/bmri/8826168","DOIUrl":"10.1155/bmri/8826168","url":null,"abstract":"<p><p><b>Background:</b> The ubiquitous nature of data/information in healthcare has made it an imperative facet that requires the services of highly trained professionals with well-endowed field competencies to properly generate and use this sensitive data to enhance healthcare outcomes. There are still numerous challenges regarding the quality of data generated in the healthcare sector, especially in many middle-income countries. A growing number of studies show that data quality issues can be linked to the repercussions of inadequate competency levels of some healthcare professionals (HCPs). In that vein, this study was purported to assess the competency level of HCPs regarding the generation and usage of health information. <b>Method:</b> A quantitative cross-sectional design was employed for the study, where professionals provided self-ratings of their competencies by completing the structured questionnaire. The study saw a response rate of 98% with 877 HCPs from eight selected health facilities in Ghana. The reliability of the study construct was tested using a Cronbach's alpha test. The competency level of the professionals was measured on a scale of 1-3 under nine competency areas and categorized into entry, intermediate, and advanced levels. The chi-square test (<i>χ</i> <sup>2</sup>) and Cramer's <i>V</i> test were used to determine the possibility of any predictive factors associated with the professionals' competency levels. An ANOVA and a Dunnett's T3 post hoc test were deployed to ascertain the significant differences in the competency levels attained in the various healthcare facilities involved in the study. All statistical tests resulting in a <i>p</i> value less than 0.05 were deemed significant. <b>Results:</b> With a target of 2.30/3.00, HCPs were only found to be mostly competent (advanced level) in the application of health information law and ethics when generating and using health information (2.50) and generic professional skills (2.33). On the contrary, HCPs had low levels of competency in the application of healthcare terminologies and disease classification (1.83), research methods skills (1.94), health service organization and delivery skills (1.96), health information and service organization management skills (2.00), the use of the language of health (2.00), electronic health skills (2.06), and health information records and management skills (2.27). Health information officers and doctors were the only professional categories that attained the threshold in our study. Sex, type of profession, educational level, and years of experience were all identified as significant predictive factors of HCP competency level. There were significant differences in the competency levels of HCPs in various facilities. <b>Conclusion:</b> There are lapses in competency levels about some specific areas which ought to be taken into cognizance. This study concludes that years of experience and educational level are the greatest predictiv","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"8826168"},"PeriodicalIF":2.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RETRACTION: Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALB/c Mice Model.","authors":"BioMed Research International","doi":"10.1155/bmri/9819461","DOIUrl":"https://doi.org/10.1155/bmri/9819461","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2014/563930.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9819461"},"PeriodicalIF":2.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanzhen Lu, Yunfei Yang, Dan Yu, Yuxi Tan, Gang Feng, Liquan Ouyang, Lulu Tan, Yuyan Tan
{"title":"Deciphering Circadian Rhythm-Related Molecular Subtypes in Breast Cancer and Establishing a Prognostic Prediction Model.","authors":"Yanzhen Lu, Yunfei Yang, Dan Yu, Yuxi Tan, Gang Feng, Liquan Ouyang, Lulu Tan, Yuyan Tan","doi":"10.1155/bmri/9664238","DOIUrl":"10.1155/bmri/9664238","url":null,"abstract":"<p><p><b>Background:</b> Recent evidence suggests that alterations in circadian rhythm genes may lead to circadian rhythm disruption (CRD), which is a key mechanism in the progression of breast cancer. Therefore, investigating the role of circadian rhythm genes in the prognosis of breast cancer holds significant clinical value. <b>Materials and Methods:</b> We utilized expression profile data from the Gene Expression Omnibus (GEO) database to identify cancer features closely associated with CRD in breast cancer. Then, we analyzed publicly available datasets (including GEO, TCGA, and METABRIC) to identify alterations in core circadian genes significantly associated with patient survival across breast cancer and constructed a circadian-related gene signature (CGS) based on these prognostic cancer features. <b>Results:</b> Circadian rhythm-related genes (CRGs) were selected to construct a risk gene signature associated with individual prognosis, which was validated in six independent cohorts and demonstrated good predictive ability. We identified three circadian rhythm-associated subtypes with distinct prognoses, which exhibited significant differences in immune checkpoint molecules, drug sensitivity, and molecular features. Additionally, the gene signature and clinicopathologic features were integrated to develop a risk model with enhanced predictive accuracy. To validate the functional role of signature genes, BMAL1 knockdown in SKBR3 cells disrupted circadian rhythms, with qPCR confirming altered risk gene expression. We found that the nomogram exhibited superior discriminative ability compared to the traditional breast cancer staging system. <b>Conclusion:</b> We developed a nomogram that can accurately predict the prognosis of breast cancer, and conclude that the expression of CRGs is crucial in breast cancer treatment decisions.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9664238"},"PeriodicalIF":2.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12419142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Corrigendum to \"The Mechanical Interpretation of Ocular Response Analyzer Parameters\".","authors":"","doi":"10.1155/bmri/9781847","DOIUrl":"10.1155/bmri/9781847","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2019/5701236.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9781847"},"PeriodicalIF":2.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alkhair Adam Khalil Mohamed, Tamekloe Woasiedem, Philip Collins Appiah-Ofori, Esraa Mohammed Abdulmahmoud Adam, Samuel Asiamah Obiri, Lawrence Sheringham Borquaye, Christian K Adokoh, Ghazi Elamin, Isaac Asiamah
{"title":"In Silico Investigation of Phytochemicals From Clinically Tested Herbal Extracts as Potential Dihydrofolate Reductase Inhibitors for Buruli Ulcer.","authors":"Alkhair Adam Khalil Mohamed, Tamekloe Woasiedem, Philip Collins Appiah-Ofori, Esraa Mohammed Abdulmahmoud Adam, Samuel Asiamah Obiri, Lawrence Sheringham Borquaye, Christian K Adokoh, Ghazi Elamin, Isaac Asiamah","doi":"10.1155/bmri/4196295","DOIUrl":"10.1155/bmri/4196295","url":null,"abstract":"<p><p>Buruli ulcer (BU), a neglected tropical disease caused by <i>Mycobacterium ulcerans</i>, requires more effective and accessible treatments. Clinical trials have shown the efficacy of herbal formulations containing <i>Nicotiana tabacum</i>, <i>Mangifera indica</i>, <i>Carica papaya</i>, and <i>Solanum rugosum</i>, although the active phytochemicals remain unstudied. In the present study, we employed an <i>in silico</i> approach to identify the active phytochemicals from these plants that could inhibit <i>M. ulcerans</i> dihydrofolate reductase (<i>Mu</i>DHFR), a crucial enzyme for mycobacterial survival. A library of 172 phytochemicals was screened using molecular docking, followed by 300-ns molecular dynamics (MD) simulations using AMBER for the top hits and standard drugs (methotrexate and trimethoprim). Binding free energies were calculated using the molecular mechanics/generalized born surface area (MM/GBSA) method. The extended MD simulations and post hoc MM/GBSA analysis revealed that kaempferol-3-O-rutinoside (<i>Δ</i> <i>G</i> <sub>bind</sub> -51.62 kcal/mol) and neochlorogenic acid (<i>Δ</i>G<sub>bind</sub> -49.44 kcal/mol) exhibited the strongest and most stable binding to <i>Mu</i>DHFR. The binding energies were more favorable than both methotrexate (<i>Δ</i> <i>G</i> <sub>bind</sub> -44.18 kcal/mol) and trimethoprim (<i>Δ</i> <i>G</i> <sub>bind</sub> -41.01 kcal/mol) used as positive controls. Stability analyses (RMSD and RMSF) and principal component analysis (PCA) confirmed that these compounds form stable complexes and induce specific conformational states in the enzyme. Favorable ADMET profiles, particularly the lack of predicted skin sensitization, support their potential for topical use. This study identifies kaempferol-3-O-rutinoside and neochlorogenic acid as the most promising lead candidates for developing novel BU therapies, warranting their prioritization for experimental validation.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"4196295"},"PeriodicalIF":2.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Restoring Tamoxifen Sensitivity in Breast Cancer: The Role of lncRNA MALAT1 and NanoCurcumin as Modulators of Drug Resistance.","authors":"Fatemeh Nasiri Kenari, Masoumeh Saberian, Matthew Abikenari, Safa Najafi, Majid Sadeghizadeh","doi":"10.1155/bmri/5824748","DOIUrl":"10.1155/bmri/5824748","url":null,"abstract":"<p><p><b>Background:</b> Tamoxifen resistance remains a major clinical challenge in estrogen receptor-positive (ER+) breast cancer, contributing to recurrence and poor prognosis. Long noncoding RNAs (lncRNAs), including MALAT1, UCA1, CYTOR, GAS5, and HOTAIR, have emerged as key regulators of endocrine resistance. Curcumin, a polyphenol with anticancer properties, modulates lncRNA expression, and its bioavailable formulation, NanoCurcumin, enhances therapeutic efficacy. This study evaluates the effects of NanoCurcumin in combination with tamoxifen on lncRNA expression and resistance mechanisms in ER+ breast cancer. <b>Methods:</b> Plasma levels of the selected lncRNAs were assessed via qRT-PCR in luminal breast cancer patients receiving tamoxifen alone or in combination with NanoCurcumin oral soft gels for 6 months. Bioinformatics analysis of MALAT1 expression was performed using the GEO database. In vitro, MALAT1 expression was evaluated in breast cancer (MCF7) and normal breast (MCF10) cell lines via qRT-PCR. Tamoxifen-resistant MCF7 cells were generated through prolonged treatment, and the effects of NanoCurcumin on MALAT1 expression were analyzed over 4 months. <b>Results:</b> In clinical samples, NanoCurcumin significantly reduced MALAT1 expression (<i>p</i> = 0.02) and trended toward decreased UCA1, CYTOR, and HOTAIR while increasing GAS5 expression. Bioinformatics analysis confirmed MALAT1 upregulation in tamoxifen-resistant cell lines. In vitro, MALAT1 was significantly elevated in MCF7 cells compared to MCF10 and increased over time with tamoxifen treatment alone. NanoCurcumin reversed this trend, sustaining low MALAT1 levels and mitigating resistance. <b>Conclusion:</b> Our findings suggest that NanoCurcumin mitigates tamoxifen resistance by downregulating MALAT1, offering a novel epigenetic strategy to enhance endocrine therapy efficacy. Further studies should explore lncRNA-targeted interventions to improve treatment outcomes in ER+ breast cancer.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"5824748"},"PeriodicalIF":2.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chunyan Lei, Feipeng Jiang, Li Zhang, Qibo Ran, Yun Zhang, Meixia Zhang, Deokho Lee
{"title":"The Association Between Vitamin D Deficiency and Nondiabetic Retinopathy in the American Population: National Health and Nutrition Examination Survey 2005-2008.","authors":"Chunyan Lei, Feipeng Jiang, Li Zhang, Qibo Ran, Yun Zhang, Meixia Zhang, Deokho Lee","doi":"10.1155/bmri/2828949","DOIUrl":"10.1155/bmri/2828949","url":null,"abstract":"<p><p><b>Objectives:</b> Retinopathy is a vascular endothelial injury disease that can occur in individuals without diabetes. The prevalence rates of nondiabetic retinopathy (NDR) vary from 6% to 13.6% among individuals. Vitamin D deficiency (VDD) is common worldwide, and studies indicate that the overall prevalence rate of VDD in US adults is 41.6%. Ample evidence indicates an inconsistent relationship between VDD and diabetic retinopathy, but the association between VDD and NDR remains limited. <b>Design:</b> We conducted a population-based, cross-sectional study. <b>Settings:</b> The study was conducted using data from the National Health and Nutrition Examination Survey 2005-2008. <b>Participants:</b> A total of 4076 adults (52.71% female) with a mean age of 55.79 ± 11.72 years were included. <b>Primary and Secondary Outcomes:</b> The primary outcome was the association between vitamin D and NDR, while there was no secondary outcome. <b>Results:</b> Retinopathy was detected in 309 nondiabetic subjects (7.6%), while VDD was detected in 19.36% of the NDR participants. In the univariate analysis, significant associations were found between systolic blood pressure (odds ratio [OR]: 1.02; 95% confidence interval (CI): 1.00, 1.04; <i>p</i> = 0.0227), physical activity group (OR: 0.63; 95% CI: 0.51, 0.78; <i>p</i> = 0.0001), and retinopathy in the nondiabetic participants. Logistic regression analysis revealed that after adjusting for other confounders, no statistically significant association between vitamin D concentration and NDR severity was found (OR: 1.02; 95% CI: 0.97; 1.06; <i>p</i> = 0.9024). Similarly, smooth curve fitting could not find any trend between the two. Moreover, these results were consistent with the results of taking vitamin D (quartile) as a categorical variable (<i>p</i> for trend was 0.8401). <b>Conclusion:</b> In the present study, serum vitamin D concentrations within the observed range were not significantly associated with NDR risk in the nondiabetic US population, indicating that vitamin D status is unlikely to be a primary determinant of subclinical microvascular pathology in nondiabetic adults.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"2828949"},"PeriodicalIF":2.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seth Offei Addo, Margaret Owusu-Akyaw, Ronald Essah Bentil, Stacy Amoah, Seth Oware, Bernice Olivia Ama Baako, John Asiedu Larbi
{"title":"Intestinal Parasitic Infections in Ghana: A Review of Infections and the Risk of Zoonotic Transmission.","authors":"Seth Offei Addo, Margaret Owusu-Akyaw, Ronald Essah Bentil, Stacy Amoah, Seth Oware, Bernice Olivia Ama Baako, John Asiedu Larbi","doi":"10.1155/bmri/7766777","DOIUrl":"10.1155/bmri/7766777","url":null,"abstract":"<p><p>Intestinal parasites pose a risk to public health globally, causing a high economic burden in developing countries. Most diagnostic methods aimed at detecting these parasites in hospital settings are not sensitive to recovering parasites. Hence, the prevalence of these diseases remains a challenge in areas of poor environmental sanitation and inadequate diagnostic tools. Control strategies are critical but may need a deep understanding of the epidemiology of parasitic diseases. In Ghana, intestinal parasitic infections are considered to be an important public health issue. Despite efforts to reduce the burden of infections, these parasites continue to spread. As such, this review is aimed at bringing up to date the existing information on the burden of intestinal parasites in Ghana by compiling available publications from 1975 to 2023. Emphasis is made on parasitic infections in vulnerable groups, livestock and the risk of zoonotic transmission. There is a need for more surveillance efforts to determine the dynamics of infections as well as intensify mass education.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"7766777"},"PeriodicalIF":2.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nidal Jaradat, Mustafa Ghanim, Mohammed Hawash, Johnny Amer, Malik Alqub, Belal Rahhal, Majdi Dwikat, Fatimah Hussein, Linda Issa, Maha Rabayaa, Mohammad Abuawad, Mohammad Halayqa, Wad Zaghloul, Esraa Hamdan
{"title":"Investigating of the Phytoconstituents, Anti-<i>α</i>-Glucosidase, Antilipase, Anti-<i>α</i>-Amylase, and DPPH Radical Scavenging Activities of Extracts From <i>Eriobotrya japonica</i>.","authors":"Nidal Jaradat, Mustafa Ghanim, Mohammed Hawash, Johnny Amer, Malik Alqub, Belal Rahhal, Majdi Dwikat, Fatimah Hussein, Linda Issa, Maha Rabayaa, Mohammad Abuawad, Mohammad Halayqa, Wad Zaghloul, Esraa Hamdan","doi":"10.1155/bmri/8888528","DOIUrl":"10.1155/bmri/8888528","url":null,"abstract":"<p><p>Recent research reported inhibitory effects of <i>Eriobotrya japonica</i> leaf extracts on DPPH free radicals and <i>α</i>-amylase, <i>α</i>-glucosidase, and lipase enzymes. These enzymes were linked to the etiology of diabetes mellitus, obesity, and oxidative stress. The current study is aimed at determining the phytocontents of <i>E. japonica</i> aqueous extract leaves and exploring their potential antioxidant, anti-<i>α</i>-amylase, anti-<i>α</i>-glucosidase, and antilipase activity using reference phytochemical and biochemical assays. The phytochemical tests on the <i>E. japonica</i> aqueous extract confirmed the presence of alkaloids, glycosides, tannins, saponins, phytosteroids, carbohydrates, and phenols. The total phenol and tannin contents of the <i>E. japonica</i> aqueous extract were 21.64 ± 0.89 mg of GAE/g and 1.72 ± 1 mg of CAE/g, respectively. <i>E. japonica</i> aqueous extract had inhibitory effects on DPPH free radicals (IC<sub>50</sub> of 7.7 ± 3.11 <i>μ</i>g/mL) and <i>α</i>-amylase (IC<sub>50</sub> = 141 ± 0.35 <i>μ</i>g/mL) compared with Trolox and acarbose (6.3 ± 0.12 and 28.18 ± 1.22 <i>μ</i>g/mL, respectively). Moreover, <i>E. japonica</i> aqueous extract inhibited <i>α</i>-glucosidase (IC<sub>50</sub> = 39.81 ± 0.74 vs. 37.15 ± 0.33 <i>μ</i>g/mL inhibition by acarbose) and lipase (IC<sub>50</sub> = 316.2 ± 0.87 vs. 12.3 ± 0.33 <i>μ</i>g/mL inhibition by orlistat). In conclusion, the current results suggest that <i>E. japonica</i> aqueous extract possesses strong antioxidant, anti-<i>α</i>-glucosidase, anti-<i>α</i>-amylase, and antilipase activities with potential applications in the treatment and prevention of obesity, diabetes mellitus, and oxidative stress.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"8888528"},"PeriodicalIF":2.3,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}