BioMed Research International最新文献

{"title":"Exploring the Impact of miR-34b-5p on BRD4 Gene Expression in Triple-Negative Breast Cancer Cells.","authors":"Mohannad Abdulameer Ibrahim, Hanieh Jalali, Mahnaz Azarnia","doi":"10.1155/bmri/7538031","DOIUrl":"10.1155/bmri/7538031","url":null,"abstract":"<p><p>The miR-34 family is recognized for its crucial role as tumor suppressors, particularly through its interactions with oncogenic regulators such as Tumor Protein 53 (TP53). Bromodomain-containing Protein 4 (BRD4) functions as a transcriptional regulator that enhances the expression of oncogenes. In triple-negative breast cancer (TNBC), BRD4 is often found to be overexpressed and linked to poor clinical outcomes. This study is aimed at exploring the impact of miR-34b on the sensitivity of TNBC cells to BRD4 inhibition, which may also affect TP53 expression. miR-34b-5p mimics and scrambled oligonucleotides were transfected into TP53-mutant MDA-MB-231 and nonmutant MCF-7 cell lines. The expression levels of miR-34b, TP53, and BRD4 genes, along with the migration rates and sensitivity to the BRD4-specific inhibitor JQ1, were compared between the miR-34b overexpressing cells. The results showed that miR-34b overexpression led to increased TP53 expression in MDA-MB-231 cells, while a reduction was observed in MCF-7 cells. Consequently, BRD4 expression was significantly elevated in MDA-MB-231 cells, resulting in resistance to JQ1. The increase in BRD4 expression also correlated with higher migration rates compared to MCF-7 cells. In conclusion, miR-34b may have an oncogenic role by promoting the expression of BRD4 in TNBC cells. This finding aligns with prior reports indicating a negative correlation between miR-34b levels and survival rates in patients with TNBC. These insights may provide new perspectives on the role of miR-34b in the development and progression of TNBC.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"7538031"},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-AHNAK1 Antibodies Are a Novel Diagnostic Biomarker for Systemic Lupus Erythematosus.","authors":"Yasushi Matushita, Kazuhisa Nozawa, Kentaro Doe, Yoshinari Takasaki, Ken Yamaji, Naoto Tamura","doi":"10.1155/bmri/6381475","DOIUrl":"10.1155/bmri/6381475","url":null,"abstract":"<p><p>Calcium signaling is essential for the proper function of immune cells. Recent studies have shown that the scaffold protein, AHNAK1, is important for efficient calcium signaling and NFAT activation in T cells through its ability to properly localize calcium ion (Ca²⁺) channels at the plasma membrane. Interestingly, both T cells and B cells of systemic lupus erythematosus exhibit activation signaling anomalies with dysregulated Ca²⁺ response, and enhanced Ca²⁺ signaling has emerged as a target for treatment with SLE. Therefore, we hypothesized SLE patients may have autoantibodies (Abs) against AHNAK1 because anti-AHNAK1 antibodies possibly are able to interfere with Ca²⁺ signaling through binding to AHNAK1, subsequently resulting in aberrant T cells signal transduction. In the present study, we notably found that sera from SLE patients profoundly elicit immunoreaction against AHNAK1 when compared to normal healthy controls (NHCs) or patients with other systemic autoimmune diseases, such as polymyositis/dermatomyositis (PM/DM), systemic sclerosis (SSc), Sjögren's syndrome (SjS), mixed connective tissue disease (MCTD), and rheumatoid arthritis (RA). Additionally, the expression level of AHNAK1 in peripheral blood mononuclear cells (PBMCs) from SLE patients was significantly increased compared to NHCs. We propose that measurement of serum anti-AHNAK1 antibodies can be used as a possible biomarker for the diagnosis of SLE. In addition, our data suggest that AHNAK1 antibodies may have an indicative role in the pathogenesis of SLE.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6381475"},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Chitosan Persian Gum Hydrogel Containing Jaft and Yarrow Extracts for Enhanced Wound Healing.","authors":"Parisa Ahmadi, Maryam Jalili Tabaii, Zahra Kanannejad, Milad Mohkam","doi":"10.1155/bmri/5592896","DOIUrl":"10.1155/bmri/5592896","url":null,"abstract":"<p><p>This study presents the development and evaluation of a novel chitosan/Persian gum hydrogel loaded with an internal layer of oak fruit (Jaft) and yarrow extracts, designed to enhance wound healing. The hydrogel was synthesized using chitosan and Persian gum, selected for their biocompatibility and ability to promote wound healing. The incorporation of Jaft and yarrow extracts was aimed at augmenting the hydrogel's therapeutic efficacy. Characterization of the hydrogel using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) confirmed the successful integration of the extracts and revealed a semicrystalline structure with a porous morphology conducive to controlled drug release. In vitro studies demonstrated a pH-responsive release profile, with faster drug release at alkaline pH (8.5) compared to acidic and neutral pH (5.5 and 7.4). The hydrogel exhibited significant antibacterial activity, particularly against <i>Escherichia coli</i>, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values lower than those observed for <i>Staphylococcus aureus</i>. Animal studies further validated the hydrogel's efficacy, showing enhanced wound closure, increased epidermal thickness, higher fibroblast proliferation, and reduced infection rates compared to controls. These findings suggest that the chitosan/Persian gum hydrogel, enriched with natural extracts, holds significant potential as a multifunctional wound dressing, offering a synergistic approach to infection control and tissue regeneration.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"5592896"},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted Applications of Synthesized Silver Nanoparticles From Marine Fungus <i>Aspergillus flavus</i> MK4: Antimicrobial, Anticancer, and Wound-Healing Properties.","authors":"Mohamed H Al-Agamy, Mahmoud S Kelany, Sultan Alshehri, Mohammad R Alhuzani, Moaz M Hamed, Wael Sabra, Wael A Mahdi","doi":"10.1155/bmri/7161915","DOIUrl":"10.1155/bmri/7161915","url":null,"abstract":"<p><p>Due to their wide range of applications in modern technology, silver metallic nanomaterials have garnered considerable attention, driving significant research and development efforts. This study focuses on investigating the antimicrobial properties of silver nanoparticles (Ag-NPs), encompassing their production, characterization, and biological aspects. The eco-friendly extracellular biosynthetic method employed in this work utilizes extracts from the marine fungus <i>Aspergillus flavus</i> MK4 as reducing agents for nanoparticle synthesis. The analysis of colloidal Ag-NPs using ultraviolet-visible spectroscopy revealed a peak in absorbance at 460 nm, indicating the presence of plasmonic properties. Electron imaging of the internal structure (TEM) elucidated the spherical shape of Ag-NPs, which measure 15 nm in size. Testing against bacterial diversities revealed potent antibacterial and antifungal efficacy. Cytotoxicity against the HepG-2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. Based on their conceivable applications in the fields of antibacterial, anticancer, and wound healing, the synthesized Ag-NPs exhibit promising features. With its powerful ability to synthesize Ag-NPs, <i>A. flavus</i> MK4 can generate extensively characterized nanoparticles, demonstrating their antibacterial, antioxidant, anticancer, and wound-healing properties.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"7161915"},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Stat3 Upregulates Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 4 Expression in Osteosarcoma Cells.","authors":"BioMed Research International","doi":"10.1155/bmri/9865234","DOIUrl":"10.1155/bmri/9865234","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2013/310691.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9865234"},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Nanohydroxyapatite and Silver Nanoparticle Incorporation on the Flexural Strength of Resin Composites.","authors":"Marzie Moradaian, Maryam Saadat, Shahab Agharezaei, Zahra Khorshidi Asl","doi":"10.1155/bmri/9132555","DOIUrl":"10.1155/bmri/9132555","url":null,"abstract":"<p><p>This in vitro study investigated the impact of silver nanoparticles (AgNPs) and nanohydroxyapatite (nanoHAP) on the flexural strength of dental composite resin. Fifty composite samples were prepared in five groups (<i>n</i> = 10): (1) nonspatulated composite resin (negative control); (2) hand-spatulated composite resin (positive control); (3) composite resin and 2 wt% nanoHAP; (4) composite resin and 1 wt% AgNPs; and (5) composite resin, 2 wt% nanoHAP, and 1 wt% AgNPs. Flexural strength was measured using a universal testing machine. Scanning electron microscopy (SEM) was used to evaluate the dispersion of nanoparticles in the composite resin. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests (<i>p</i> < 0.05 was considered significant). The highest mean flexural strength was observed in the negative control, while the lowest flexural strength was seen in Group 4 (composite resin and AgNPs). The nanoHAP group demonstrated significantly higher flexural strength than the AgNP group (<i>p</i> = 0.043). However, no significant difference was observed between the nanoHAP-only group and the group containing both nanoHAP and AgNPs (<i>p</i> = 0.075). These findings suggest that nanoHAP-loaded composite resin possesses higher flexural strength than AgNP-incorporated composite resin. Additionally, comparisons with the positive control group indicated that the addition of nanoparticles did not necessarily result in significant improvement in flexural strength. These findings can pave the way for future studies on optimizing the composition of advanced dental materials with enhanced longevity and improved mechanical properties.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9132555"},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Exendin-4 Attenuates Hepatic Steatosis by Promoting the Autophagy-Lysosomal Pathway\".","authors":"","doi":"10.1155/bmri/9793841","DOIUrl":"https://doi.org/10.1155/bmri/9793841","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2022/4246086.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9793841"},"PeriodicalIF":2.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of Malaria Among Pregnant Women at a Tertiary Care Hospital in Somalia: A Cross-Sectional Study.","authors":"Said Mohamed Mohamud, Serpil Doğan, Ahmed Issak Hussein, Rahma Yusuf Haji Mohamud, Zerife Orhan, Adem Doğaner","doi":"10.1155/bmri/6730167","DOIUrl":"10.1155/bmri/6730167","url":null,"abstract":"<p><p><b>Background:</b> In countries like Somalia, where health infrastructure is inadequate and malaria is endemic, immunosuppression during pregnancy increases the risk of placental malaria; this, in turn, leads to anemia, low birth weight, preterm delivery, and stillbirth, causing severe complications that pose a life-threatening risk to both the mother and fetus. <b>Aim:</b> The aim of this study was to investigate the prevalence and associated factors of malaria parasitemia among pregnant women attending the obstetric clinic of a tertiary hospital in Somalia. <b>Method:</b> This cross-sectional study, conducted from November 2022 to January 2023 at a tertiary hospital in Mogadishu, involved 398 pregnant women. Both symptomatic and asymptomatic participants were screened for malaria using Giemsa-stained blood smear microscopy. Associations between variables and malaria prevalence were analyzed using IBM SPSS 22 with chi-square tests and logistic regression. <b>Results:</b> Among 398 pregnant women, 238 (59.8%) tested positive for malaria. Of the 238 malaria cases, 218 (91.6%) were revealed as <i>Plasmodium falciparum</i> cases. Being in the second trimester (OR: 0.524, 95% CI: 0.279-0.983; <i>p</i> = 0.044), third trimester (OR: 0.442, 95% CI: 0.245-0.797; <i>p</i> = 0.007), and indoor residual spraying (IRS) (OR: 0.192, 95% CI: 0.108-0.342; <i>p</i> < 0.001) were significantly associated with decreased odds of malaria in pregnancy. <b>Conclusion:</b> The study revealed a high prevalence of malaria in pregnancy, predominantly caused by <i>Plasmodium falciparum</i>. Targeted education campaigns focusing on women in the second trimester and those not using preventive measures such as IRS or ITNs should be prioritized, along with routine malaria screening at every antenatal care visit.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6730167"},"PeriodicalIF":2.3,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy and Reproducibility of Semidigital Versus Fully Digital Cephalometric Tracings Using a New Computer Program Versus Conventional Methods (Gold Standards): A Preliminary Study.","authors":"Farhad Sobouti, Sepideh Dadgar, Sina Namadian, Hamid Reza Bahrami Rad, Vahid Rakhshan","doi":"10.1155/bmri/8403357","DOIUrl":"https://doi.org/10.1155/bmri/8403357","url":null,"abstract":"<p><p><b>Introduction:</b> Cephalometric tracing can be done either conventionally or using computers. Digital dentistry and digital orthodontics have considerably facilitated procedures. Still, their diagnostic accuracy needs assessment. Many orthodontic programs have been developed for this purpose. The efficacy and reliability of such software are usually compared with the conventional method (gold standard). We used novel and more stringent methods of assessment to test a program in this regard. <b>Methods:</b> This study was performed on 10,302 tracing evaluations within 101 cases. Lateral cephalograms of 101 patients were landmarked using two methods (on paper vs. on a computer screen) and traced using three methods (completely conventionally [gold standard]; landmarks were identified on paper, but measurements were calculated by computer; landmarks were identified on the computer screen, and measurements were calculated by the computer program). A total of 15 landmarks and 17 cephalometric tracing measurements were determined via the abovementioned methods. The tracing errors were defined as differences between each pair of tracing methods, as well their absolute values (a total of 6 different tracing errors). Intraclass correlations were calculated for tracing values. Tracing errors were compared with the value 2, as the clinically acceptable range. However, they were also compared with the values zero as well as one hundredth of the mean of gold standard (as a more conservative value), using a one-sample <i>t</i>-test (<i>α</i> = 0.05). <b>Results:</b> All tracing errors were smaller than the clinically acceptable limits. Moreover, most simple errors were close to zero, and/or below the criterion of 1/100 of the mean of the gold standard. Furthermore, the more difficult error tests, that is, the directionless absolute errors, were all below 2; additionally, they were either below the 1/100 of absolute of the gold standard means or at the level of those means. Finally, the intraobserver reliabilities were high. All the 102 simple errors and absolute errors (on 101 lateral cephalograms) were significantly below 2 (<i>p</i> < 0.0005, clinically acceptable). <b>Conclusions:</b> The accuracy was appropriate. Of the 51 simple tracing errors, only 9 were significantly greater than zero, and many of them were below or at the level of 1/100 of the gold standard means. All the directionless (absolute) errors were significantly greater than zero. However, in the case of those calculated as \"absolute value of (gold standard subtracted by fully digital method),\" all errors were below or at the level of 1/100 of the absolute of gold standards' means. The intraobserver reliabilities were high.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"8403357"},"PeriodicalIF":2.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IER5 Negatively Regulates Cdc25B Expression in HeLa Cells After Gamma Ray Irradiation.","authors":"Xianzhe Zhao, Lixin Ding, Yongzhong Ma, Wei Cheng, Li Zhang, Kuke Ding","doi":"10.1155/bmri/5644318","DOIUrl":"https://doi.org/10.1155/bmri/5644318","url":null,"abstract":"<p><p>Radiotherapy is commonly used to treat many cancers, and their sensitivity to radiation is crucial for favorable outcomes. This study investigated whether the immediate early response 5 (IER5) protein affects Cdc25B protein expression in HeLa cells after gamma irradiation. IER5 was knocked down using RNA interference (RNAi) in HeLa cells irradiated with 2 or 4 Gy of gamma rays. The mRNA and protein expression levels were subsequently determined via qRT-PCR and western blotting. The distribution of cells during the cell cycle was also determined using flow cytometry. IER5 protein levels were successfully reduced with RNAi. Variations in IER5 levels led to differences in Cdc25B mRNA and protein levels. Moreover, IER5 affected the proportion of cells in the G2 phase, which is regulated mainly by Cdc25B. Pearson correlation analysis was conducted on the expression levels of IER5 and Cdc25B in HeLa cells and the IER5-silenced HeLa (siIER5-HeLa) cell line at various time points after exposure to 4 Gy of gamma rays, and a negative correlation was detected between IER5 and Cdc25B expression levels, with correlation coefficients of -0.686 and -0.663, respectively. Additionally, variations in IER5 levels led to differences in the expression levels of p53, NF-YB, and p300, which may be putative transcriptional regulators of Cdc25B. These results suggest that IER5 plays a negative role in regulating Cdc25B expression, which may involve interactions with the transcriptional regulators p53, NF-YB, and p300.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"5644318"},"PeriodicalIF":2.3,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}