BioMed Research International最新文献

{"title":"Etiologic Profile of the Pneumococcus in Ghana: A Systematic Review.","authors":"Reuben E Arhin, Eric S Donkor, Hans-Christian Slotved, Fleischer C N Kotey, Nicholas T K D Dayie","doi":"10.1155/2024/8368996","DOIUrl":"10.1155/2024/8368996","url":null,"abstract":"<p><p><b>Objective:</b> To describe the profile of <i>Streptococcus pneumoniae</i>, identify research gaps, and provide in-depth insights into various aspects related to the pathogen. <b>Methods:</b> Google Scholar, PubMed, and ScienceDirect were searched for all studies on the pneumococcus in Ghana that reported on specimen collected, population and sample size, carriage prevalence, incidence of pneumococcal diseases, age of the study population, types of test performed, serotypes identified, antimicrobial susceptibilities, or molecular analysis on the pneumococci for data extraction. <b>Results:</b> Overall, a total of 7954 results were obtained from the three-database search, and of this, 24 articles were selected after screening. A total of 924 isolates were accounted for by serotyping/serogrouping. The prevalence of pneumococcal carriage in Ghana ranges from 11.0% to 51.4% in the population depending on the age (≤ 24-80 years), sickle cell disease (SCD), human immunodeficiency virus (HIV) status, or health of the study population, and penicillin (Pen)-nonsusceptible isolates ranged from 17% to 63%. The prevalence of pneumococci found as the etiologic agent of diseases among Ghanaians ranges from 3.4% for otitis media to 77.7% for meningitis. Overall, the 13-valent pneumococcal conjugate vaccine (PCV) (PCV-13) carriage serotypes accounted for 28.4% of the reported pneumococcal isolates. PCV-13 invasive serotypes accounted for 22.4% of the reported isolates. The non-PCV-13 carriage serotypes accounted for most (43.9%) of the reported isolates. In the pre-PCV-13 era, the nontypeable (NT) (5.5%) and other nonvaccine types (NVTs) (6.4%) were reported as being predominant. The non-PCV-13 serotypes accounted for 4.4% of the reported isolates in invasive pneumococcal disease (IPD) cases. Multidrug resistance (MDR) ranged from 7.8% to 100%. <b>Conclusion:</b> Predicting the invasiveness of pneumococci using molecular typing is the way to go in the future as this will provide answers to the extent to which capsular switching is contributing to the pneumococcal disease burden in Ghana almost a decade after introducing PCV-13. Continuous monitoring of antibiotic resistance patterns at both phenotypic and genotypic levels, along with serotyping and molecular typing, should be a standard practice in the surveillance of pneumococcal disease burden in Ghana.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Zirconia Adhesion: Pre and Postsintering Physical Surface Treatment, Chemical Treatment, and Cement Interactions.","authors":"Flávia Gonçalves, Mirko Dennys Ayala-Perez, Francisco Carlos Dos Santos Reis, Walter Gomes Miranda-Júnior, Letícia Cristina Cidreira Boaro","doi":"10.1155/2024/5394652","DOIUrl":"10.1155/2024/5394652","url":null,"abstract":"<p><p><b>Background:</b> Adhesion to zirconia remains a significant dental challenge. This study is aimed at assessing the bond strength of zirconia based on surface treatment with pre or postsintering sandblasting associated with different chemical treatments and resin cements. <b>Methods:</b> Zirconia blocks were divided into 12 experimental groups based on the surface treatment (presintering sandblasting or postsintering sandblasting/tribochemical abrasion treatment), chemical treatment (none, Single Bond Universal, or Signum Zirconia Bond), and choice of cement (Panavia F or RelyX™ U200). The bond strength was measured by shear tests using a universal testing machine. The fracture analysis was performed using stereomicroscopy. Data were analyzed using three-way ANOVA and Tukey's test (<i>α</i> = 5%). <b>Results:</b> Triple and double factor's interactions were not significant (<i>p</i> > 0.05). Regarding the surface treatment factor, the bond strength following postsintering sandblasting treatment associated with tribochemical abrasion (9.15 ± 3.62 MPa) was significantly higher than presintering sandblasting treatment (5.24 ± 3.53 MPa). Concerning the chemical treatment factor, bond strengths were ranked as follows: Signum Zirconia Bond > Single Bond Universal > no treatment. The bond strength of the resin cements did not differ among them. Most fractures (67%) were classified as adhesive, and 32% were categorized as mixed fractures. <b>Conclusion:</b> Surface treatment via postsintering sandblasting combined with tribochemical abrasion demonstrated superior efficacy than in presintering sandblasting. Additionally, chemical treatment with zirconia primer increased the bond strength of zirconia irrespective of the surface physical treatment.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Therapeutic Potential of Systemic Ozone on Skin Wound Repair: Clinical, Histological, and Immunohistochemical Study in Rats.","authors":"Jânderson de Medeiros Cardoso, Edilson Ervolino, Erton Massamitsu Miyasawa, Leticia Helena Theodoro, Luis Eduardo Marques Padovan, Estevão Lopes Pereira, Rafael Scaf de Molon, Valdir Gouveia Garcia","doi":"10.1155/2024/6623114","DOIUrl":"10.1155/2024/6623114","url":null,"abstract":"<p><p>This study sought to examine the effects of systemic ozone (O₃) treatment on the healing of skin wounds induced on the dorsal surface of Wistar rats. The skin wounds were created using a 10 mm round punch following the sagittal medial plane in 72 rats. Then, the animals were randomly assigned to four groups, each receiving the following treatments: group C, which did not undergo treatment with the O₃/O₂ mixture; group OZ0.3, administered the O₃/O₂ mixture at a dose of 0.3 mg/kg; group OZ0.7, given the O₃/O₂ mixture at a dose of 0.7 mg/kg; and group OZ1.0, provided with the O₃/O₂ mixture at a dose of 1.0 mg/kg. Six animals from each group were euthanized at 7, 14, and 21 days postoperatively. Clinical, histological, histometric, and immunohistochemical (IHC) analyses were accomplished. Data from clinical and histometric assessments revealed that OZ0.7 and OZ1.0 demonstrated more favorable healing, with greater wound contraction observed in the OZ1.0 group at 14 and 21 days. Histologically, the OZ1.0 group exhibited aspects consistent with an accelerated tissue repair process. IHC analysis revealed greater vascular endothelial growth factor (VEGF) immunostaining in the OZ0.7 (7 days) and OZ1.0 (7 and 14 days) groups compared to the C group. Expression of transforming growth factor beta-1 was significantly increased in the OZ0.7 (14 days) and OZ1.0 (7 and 14 days) groups compared to the C group. In conclusion, our data suggest that systemic use of O₃ enhanced tissue repair in cutaneous wounds in a dose-dependent manner, with concentrations of 1.0 mg/kg providing the most beneficial effects. Furthermore, the results of this study implicate the use of O₃ for the treatment of skin wounds aiming at improving the healing process over time. Our findings suggest the use of O₃ as a viable alternative to enhance wound healing and repair.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ideal Living Skin Equivalents, From Old Technologies and Models to Advanced Ones: The Prospects for an Integrated Approach.","authors":"Andrei Riabinin, Maria Pankratova, Olga Rogovaya, Ekaterina Vorotelyak, Vasiliy Terskikh, Andrey Vasiliev","doi":"10.1155/2024/9947692","DOIUrl":"10.1155/2024/9947692","url":null,"abstract":"<p><p>The development of technologies for the generation and transplantation of living skin equivalents (LSEs) is a significant area of translational medicine. Such functional equivalents can be used to model and study the morphogenesis of the skin and its derivatives, to test drugs, and to improve the healing of chronic wounds, burns, and other skin injuries. The evolution of LSEs over the past 50 years has demonstrated the leap in technology and quality and the shift from classical full-thickness LSEs to principled new models, including modification of classical models and skin organoids with skin derived from human-induced pluripotent stem cells (iPSCs) (hiPSCs). Modern methods and approaches make it possible to create LSEs that successfully mimic native skin, including derivatives such as hair follicles (HFs), sebaceous and sweat glands, blood vessels, melanocytes, and nerve cells. New technologies such as 3D and 4D bioprinting, microfluidic systems, and genetic modification enable achievement of new goals, cost reductions, and the scaled-up production of LSEs.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Gene Expression Data-Driven Identification of Molecular Signatures, Prognostic Biomarkers, and Drug Targets for Glioblastoma.","authors":"Md Wasim Alom, Md Delowar Kobir Jibon, Md Omar Faruqe, Md Siddikur Rahman, Farzana Akter, Aslam Ali, Md Motiur Rahman","doi":"10.1155/2024/6810200","DOIUrl":"10.1155/2024/6810200","url":null,"abstract":"<p><p>Glioblastoma (GBM) is a highly prevalent and deadly brain tumor with high mortality rates, especially among adults. Despite extensive research, the underlying mechanisms driving its progression remain poorly understood. Computational analysis offers a powerful approach to explore potential prognostic biomarkers, drug targets, and therapeutic agents for GBM. In this study, we utilized three gene expression datasets from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs) associated with GBM progression. Our goal was to uncover key molecular players implicated in GBM pathogenesis and potential avenues for targeted therapy. Analysis of the gene expression datasets revealed a total of 78 common DEGs that are potentially involved in GBM progression. Through further investigation, we identified nine hub DEGs that are highly interconnected in protein-protein interaction (PPI) networks, indicating their central role in GBM biology. Gene Ontology (GO) and pathway enrichment analyses provided insights into the biological processes and immunological pathways influenced by these DEGs. Among the nine identified DEGs, survival analysis demonstrated that increased expression of GMFG correlated with decreased patient survival rates in GBM, suggesting its potential as a prognostic biomarker and preventive target for GBM. Furthermore, molecular docking and ADMET analysis identified two compounds from the NIH clinical collection that showed promising interactions with the GMFG protein. Besides, a 100 nanosecond molecular dynamics (MD) simulation evaluated the conformational changes and the binding strength. Our study highlights the potential of GMFG as both a prognostic biomarker and a therapeutic target for GBM. The identification of GMFG and its associated pathways provides valuable insights into the molecular mechanisms driving GBM progression. Moreover, the identification of candidate compounds with potential interactions with GMFG offers exciting possibilities for targeted therapy development. However, further laboratory experiments are required to validate the role of GMFG in GBM pathogenesis and to assess the efficacy of potential therapeutic agents targeting this molecule.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Child Immunization Coverage in Urban Settings of Twelve Provinces Plus Kabul, Afghanistan, 2019.","authors":"Khwaja Mir Islam Saeed, Shoaib Naeemi, Ruqia Naser, Bahara Rasooly, Mir Salamuddin Hakim, Khalid Arman, Homeira Nishat","doi":"10.1155/2024/5400013","DOIUrl":"10.1155/2024/5400013","url":null,"abstract":"<p><p><b>Background:</b> Low immunization and discrepancies in data sources have been a consistent challenge in Afghanistan. The objective of this was to estimate the coverage of immunization status among children of 12-23 months in urban settings of 12 provinces plus Kabul, Afghanistan in 2019. <b>Methods:</b> A cross-sectional survey was conducted in the capital of 12 cities of polio high-risk provinces plus Kabul during October-December 2019. A two-stage cluster sampling was used to approach 30 clusters and interview seven households. The coverage for 13 vaccines against 10 childhood diseases prioritized by the Afghanistan Immunization program was assessed through observation of vaccine cards or by history from caregivers of children. Epi Info v.7.2.5 was used for data management and analysis. <b>Results:</b> Totally, 3382 caregivers of children aged 12-23 months, of whom 50.8% were boys, were interviewed. The literacy of mothers was 35%, and 86.4% were housewives with no formal employment. The average age of children was 17.07 ± 4.05 months. In total, 1261 (37.29%) children were fully vaccinated, 833 (54.2%) were partially vaccinated, and 288 (8.52%) did not receive any dose of routine vaccine. Of total, 71.82% had vaccination cards, 17.24% had lost them, and 11% had no cards. Generally, coverage of immunization by cards and history was 91.70% for BCG, 52% for Penta, 78% for OPV-4, 63% for PCV2, 61% for Rota2, 68.50% for measles 1, and 58% for IPV. Nangarhar and Kunar provinces have the highest and lowest immunization coverage, respectively. Lack of awareness and time was the main factor cited by partially vaccinated individuals, while misconceptions about vaccines were reported among the unvaccinated. <b>Conclusion:</b> Child immunization levels, varying across cities, were suboptimal in the study population. Realistic goal-setting and awareness campaigns are necessary to address the low immunization coverage and fight against barriers in Afghanistan.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of Virulence-Associated Markers in <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates From Water, Cattle, and Chicken Faecal Samples From Kajiado County, Kenya.","authors":"Daniel W Wanja, Christine M Mbindyo, Paul G Mbuthia, Lilly C Bebora, Gabriel O Aboge","doi":"10.1155/2024/4631351","DOIUrl":"10.1155/2024/4631351","url":null,"abstract":"<p><p><i>Campylobacter</i> is a zoonotic foodborne pathogen that is often linked with gastroenteritis and other extraintestinal infections in humans. This study is aimed at determining the genetic determinants of virulence-encoding genes responsible for flagellin motility protein A (<i>flaA</i>), <i>Campylobacter</i> adhesion to fibronectin F (<i>cadF</i>), <i>Campylobacter</i> invasion antigen B (<i>ciaB</i>) and cytolethal distending toxin (cdt) A (<i>cdtA</i>) in <i>Campylobacter</i> species. A total of 29 <i>Campylobacter coli</i> isolates (16 from cattle, 9 from chicken, and 4 from water samples) and 74 <i>Campylobacter jejuni</i> isolates (38 from cattle, 30 from chicken, and 6 from water samples) described in an earlier study in Kajiado County, Kenya, were examined for the occurrence of virulence-associated genes using polymerase chain reaction (PCR) and amplicon sequencing. The correlations among virulence genes were analyzed using Pearson's correlation coefficient (<i>R</i>) method. Among the 103 <i>Campylobacter</i> strains screened, 89 were found to harbour a single or multiple virulence gene(s), giving an overall prevalence of 86.4%. <i>C. jejuni</i> strains had the highest prevalence of multivirulence at 64.9% (48/74), compared to <i>C</i>. <i>coli</i> (58.6%, 17/29). The <i>ciaB</i> and <i>flaA</i> genes were the most common virulence genes detected in <i>C</i>. <i>jejuni</i> (81.1% [60/74] and 62.2% [46/74], respectively) and in <i>C. coli</i> (each at 62.1%; 18/29). <i>Campylobacter</i> isolates from chicken harboured the most virulence-encoding genes. <i>C. jejuni</i> strains from chicken and cattle harboured the highest proportions of the <i>cdtA</i> and <i>ciaB</i> genes, respectively. All the <i>C. coli</i> strains from water samples harboured the <i>cadF</i> and <i>flaA</i> genes. The results obtained further revealed a significant positive correlation between <i>cadF</i> and <i>flaA</i> (<i>R</i> = 0.733). <i>C. jejuni</i> and <i>C. coli</i> strains from cattle, chicken, and water harbour virulence markers responsible for motility/colonization, invasion, adherence, and toxin production, evoking their important role in campylobacteriosis development among humans and livestock. The identification of cattle, chicken, and water samples as reservoirs of virulent <i>Campylobacter</i> spp. highlights the possible risk to human health. These data on some virulence genes of <i>Campylobacter</i> will assist food safety and public health officials in formulating policy statements.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11335418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem Resistance in <i>Acinetobacter calcoaceticus-baumannii</i> Complex Isolates From Kathmandu Model Hospital, Nepal, Is Attributed to the Presence of <i>bla</i> _OXA-23-like and <i>bla</i> _NDM-1 Genes.","authors":"Anupama Gurung, Rajindra Napit, Basudha Shrestha, Binod Lekhak","doi":"10.1155/2024/8842625","DOIUrl":"10.1155/2024/8842625","url":null,"abstract":"<p><p>The <i>Acinetobacter calcoaceticus-baumannii</i> (ACB) complex, also known as ACB complex, consists of four bacterial species that can cause opportunistic infections in humans, especially in hospital settings. Conventional therapies for susceptible strains of the ACB complex include broad-spectrum cephalosporins, <i>β</i>-lactam/<i>β</i>-lactamase inhibitors, and carbapenems. Unfortunately, the effectiveness of these antibiotics has declined due to increasing rates of resistance. The predominant resistance mechanisms identified in the ACB complex involve carbapenem-resistant (CR) oxacillinases and metallo-<i>β</i>-lactamases (MBLs). This research, conducted at Kathmandu Model Hospital in Nepal, sought to identify genes associated with CR, specifically <i>bla</i>NDM-1, <i>bla</i>OXA-23-like, and <i>bla</i>OXA-24-like genes in carbapenem-resistant <i>Acinetobacter calcoaceticus-baumannii</i> (CR-ACB) complex. Additionally, the study is aimed at identifying the ACB complex through the sequencing of the 16s rRNA gene. Among the 992 samples collected from hospitalized patients, 43 (approximately 4.334%) tested positive for the ACB complex. These positive samples were mainly obtained from different hospital units, including intensive care units (ICUs); cabins; and neonatal, general, and maternity wards. The prevalence of infection was higher among males (58.14%) than females (41.86%), with the 40-50 age group showing the highest infection rate. In susceptibility testing, colistin and polymyxin B exhibited a susceptibility rate of 100%, whereas all samples showed resistance to third-generation cephalosporins. After polymyxins, gentamicin (30.23%) and amikacin (34.88%) demonstrated the highest susceptibility. A substantial majority (81.45%) of ACB complex isolates displayed resistance to carbapenems, with respiratory and pus specimens being the primary sources. Polymerase chain reaction (PCR) revealed that the primary CR gene within the ACB complex at this hospital was <i>bla</i> _OXA-23-like, followed by <i>bla</i> _NDM-1. To ensure the accuracy of the phenotypic assessment, 12 samples were chosen for 16s rRNA sequencing using Illumina MiSeq™ to confirm that they are <i>Acinetobacter</i> species. QIIME 2.0 analysis confirmed all 12 isolates to be <i>Acinetobacter</i> species. In the hospital setting, a substantial portion of the ACB complex carries CR genes, rendering carbapenem ineffective for treatment.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Challenges in the Development of Platelet-Rich Plasma-Based Therapies.","authors":"Jon Mercader-Ruiz, Maider Beitia, Diego Delgado, Pello Sánchez, Begoña Porras, Irene Gimeno, Sergio González, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, Mikel Sánchez","doi":"10.1155/2024/6444120","DOIUrl":"10.1155/2024/6444120","url":null,"abstract":"<p><p>Nowadays, biological therapies are booming and more of these formulations are coming to the market. Platelet-rich plasma, or PRP, is one of the most widely used biological therapies due to its ease of obtention and autologous character. Most of the techniques to obtain PRP are focusing on new processes and methods of optimization. However, not enough consideration is being given to modify the molecular components of PRP to generate more effective formulations with the aim of improving PRP treatments. Therefore, this review covers different novel PRP-obtaining methods that attempt to modify the molecular composition of the plasma.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11329313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Associated Factors of Adolescent (15-19 Years) Childbearing in Ghana.","authors":"Abdul Rauf Alhassan, Tina Wepeamo Wepeba, Kasim Abdulai, Rhubamatu Iddrisu, Gifty Apiung Aninanya","doi":"10.1155/2024/3237882","DOIUrl":"10.1155/2024/3237882","url":null,"abstract":"<p><p><b>Background:</b> Adolescent pregnancies continue to be a global issue that affects more high-income, middle-income, and then low-income countries, with the latter experiencing the majority of cases. <b>Aim:</b> The current study looked into the prevalence and variables predicting adolescent childbearing in Ghana. <b>Methodology:</b> Data from the Ghana Multiple Indicator Cluster Survey (MICS) 2017-2018 was used to conduct an analytical cross-sectional study. The results were examined with SPSS Version 20 (IBM Corp., 2011, and NY). Pearson's chi-square and binary logistics analyses were done for associations. A <i>p</i> value of 0.05 was used to determine the analysis's statistical significance. <b>Results:</b> The total number of adolescents isolated from the 2017 Ghana MICS dataset for this study analysis was 2974. The mean age of the study participants was 16.9 ± 1.4 years with a modal age of 15 years. The prevalence of adolescent childbearing according to this study analysis was 12.3%. The predictive factors for adolescent childbearing were increasing age, decreasing educational level, Volta regional originality, ethnic originality of the study participants, and low economic status. <b>Conclusion:</b> The prevalence of adolescent childbearing in this study was significant and needs the attention of all. Programs to improve adolescent reproductive health must take into account multiple levels of elements, such as the individual, family, community, institutions, national, and international challenges that have an impact on such programs.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11325008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}