BioMed Research International最新文献

{"title":"Erythroferrone-Driven Regulation of Hepcidin and Iron Levels in Polytransfused Sickle Cell Anaemia Patients: A Prospective Study.","authors":"Samuel Kwasi Appiah, Charles Nkansah, Godfred Amoah Appiah, Gabriel Abbam, Felix Osei-Boakye, Samira Daud, Kofi Mensah, Safo Adwoa, Korah Seedolf Kuwaahsuore, Emmanuel Yeboah, Abu Siraj Salma Tiyumba, Dennis Thompson, Viel Mary Paula, Louis Adda Duibajia, Peter Takyia, Franklina Ataa Kwarteng, Obed Odame Asiedu, Firdaus Ibrahim Sukasorr, Vincent Kawuribi, Boniface Nwofoke Ukwah, Ejike Felix Chukwurah","doi":"10.1155/bmri/6803051","DOIUrl":"10.1155/bmri/6803051","url":null,"abstract":"<p><p>The interplay of erythroferrone (ERFE), hepcidin, and ferroportin is crucial for ensuring systemic iron homeostasis. This study determined the influence of ERFE on hepcidin and iron levels in polytransfused patients with sickle cell anaemia (SCA). This multicentre case-control study recruited 60 SCA participants and 30 controls (HbA), aged 2-34 years, from Tamale Teaching Hospital; Methodist Hospital, Wenchi; and Seventh Day Adventist Hospital, Sunyani, Ghana, between the periods of March to July 2023. About 4 mL of blood was collected for a full blood count using a haematology analyzer and serum ERFE, hepcidin, ferroportin, and ferritin estimation using an enzyme-linked immunosorbent assay. Data were analyzed using SPSS Version 26.0. ERFE (<i>p</i> < 0.001), ferroportin (<i>p</i> = 0.016), ferritin (<i>p</i> < 0.001), serum iron (<i>p</i> < 0.001), transferrin (<i>p</i> = 0.001), soluble transferrin receptor (sTFR) (<i>p</i> = 0.019), TWBC (<i>p</i> < 0.001), and platelet (<i>p</i> < 0.001) were significantly higher in SCA participants and hydroxyurea-naïve participants than in the control group and hydroxyurea-treated participants, respectively. Levels of hepcidin (<i>p</i> < 0.001), red blood cell (<i>p</i> < 0.001), haemoglobin (<i>p</i> < 0.001), and haematocrit (<i>p</i> < 0.001) were lower in the SCA and hydroxyurea-naïve groups than in the control and hydroxyurea-treated groups, respectively. An inverse correlation was observed between serum ERFE and hepcidin (<i>r</i> = -0.391, <i>p</i> = 0.002) and hepcidin and ferroportin (<i>r</i> = -0.266, <i>p</i> = 0.040), while ferritin (<i>r</i> = 0.439, <i>p</i> < 0.001) and ferroportin (<i>r</i> = 0.309, <i>p</i> = 0.016) showed a positive correlation with ERFE. No correlation was found between serum hepcidin and ferritin levels (<i>r</i> = 0.025, <i>p</i> = 0.853). Again, participants with regular blood transfusions had significantly higher levels of ERFE (<i>p</i> < 0.001) and ferritin (<i>p</i> = 0.002) than those with rare and no transfusions per year. None of the SCA participants had done iron testing. In conclusion, the negative impact of ERFE on hepcidin levels may exacerbate the risk of iron burden, as evident by elevated iron levels in SCA patients and the need for regular monitoring of the iron status of polytransfused SCA patients.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6803051"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the Gap in Smoking Cessation: Unveiling Tobacco Harm Reduction in Pakistan Through Print Media Content Analysis.","authors":"Abdul Hameed, Daud Malik","doi":"10.1155/bmri/3822509","DOIUrl":"10.1155/bmri/3822509","url":null,"abstract":"<p><p>This paper provides an in-depth content analysis of safer smoking alternatives in Pakistani print media during a 5-year period (2018-2022). The mainstream print media in the English and Urdu languages is grappling with understanding the importance of tobacco harm reduction and, as a result, the safer nicotine alternatives as one of the options to address combustible smoking. Largely, the perception portrayed by the English and Urdu newspapers through news stories, articles, and so on regarding tobacco harm reduction and safer nicotine alternatives is negative. This may be due to the strong opposition to safer nicotine alternatives by the organizations working on tobacco control in Pakistan. However, on the other hand, there has been positive coverage of safer nicotine products, with the main message around how they can help adult smokers reduce harm to their health and ultimately quit smoking. Clearly, the print media need more awareness and education regarding tobacco harm reduction and safer nicotine alternatives. The emphasis should be on providing evidence-based information about safer nicotine alternatives and their role in addressing the issue of combustible smoking prevalence.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"3822509"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Height Growth Modeling in Ethiopian Children and Adolescents Aged 7-20 Years: A Prospective Cohort Study.","authors":"Dereje Danbe Debeko, Ayele Taye Goshu","doi":"10.1155/bmri/7288345","DOIUrl":"10.1155/bmri/7288345","url":null,"abstract":"<p><p><b>Background:</b> Modeling physical growth plays a vital role in examining and defining growth trajectories related to public health and well-being. <b>Aim:</b> The primary objective of this study was to model height growth in Ethiopian children and adolescents aged 7-20 years to estimate the growth variations across the Ethiopian regions. <b>Methods:</b> A total of 891 children and adolescents aged 7.5-20 years were included in the study. To estimate growth spurts within and between study subjects over time, the SITAR and PB1 models were fitted to the height growth measurements gathered in four survey rounds to. <b>Results:</b> Boys experienced puberty 2.6 years later than girls did, while the mean peak height velocity was estimated to be 5.5 cm/year in boys and 6.3 cm/year in girls. The mean adult height in boys was estimated to be 174.6 cm, while in girls, it was estimated to be 162.2 cm. Both girls (<i>p</i> < 0.005) and boys (<i>p</i> < 0.008) in Amhara and Tigrai regions were significantly shorter compared to their counterparts in Addis Ababa. However, there was no significant height difference between girls and boys in former SNNPRS region, Oromia region, and Addis Ababa. Height at peak velocity strongly correlated with the rate of change during the pubertal period. The rate of change in both boys and girls during the prepubertal and pubertal growth stages was inversely correlated with the adult height. <b>Conclusions:</b> Children who had rapid rate of change during the prepubertal and pubertal periods attained adulthood later in life. There was a significantly different height growth in children and adolescents across the regions of Ethiopia.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"7288345"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical Analysis of Ankle Stability Following Deltoid Ligament Repair and Reconstruction.","authors":"Ali Ahmadi Pirshahid, Pawel Brzozowski, Olawale Sogbein, Radovan Zdero, Aaron Gee, Mansur Halai, Emil Schemitsch, David W Sanders, Abdel Rahman Lawendy, Christopher Del Balso","doi":"10.1155/bmri/6313005","DOIUrl":"10.1155/bmri/6313005","url":null,"abstract":"<p><p><b>Background:</b> The deltoid ligament has been shown to contribute to the stability of the ankle mortise, preventing valgus talar tilt, external rotation (ER), and translation. Research to date assessing the appropriateness of deltoid repair to reintroduce medial stability to a fractured ankle is unclear. Deltoid ligament reconstruction using autograft or allograft has been proposed in cases of chronic deltoid insufficiency. This biomechanical study sought to assess the stability offered by deltoid repair and reconstruction compared to the native deltoid ligament in its intact and defunctioned states. <b>Materials and Methods:</b> Twelve (six pairs) fresh frozen cadaveric lower extremities with intact deltoid ligaments underwent biomechanical assessment in a custom-made multiaxial testing apparatus. Each specimen was tested in plantarflexion/dorsiflexion (PF/DF), inversion/eversion (IV/EV), and internal rotation (IR)/ER and analyzed for angular range of motion (ROM) and stiffness. The specimens were tested with an intact deltoid ligament and following disruption via transection. Subsequently, paired specimens were randomized to either deltoid repair or reconstruction. A single double-loaded suture anchor was used to repair the deep and superficial deltoid ligaments. The tibialis anterior tendon was used as an autograft in the reconstruction group. <b>Results:</b> Normalized ROM and stiffness were significantly different in deficient specimens compared to all other groups during PF/DF. During IR/ER, the deficient ankle was significantly different from the intact and repair state. For IV/EV, deficient specimen ROM was significantly greater than the intact or repair states, while the stiffness for the deficient ankles was significantly less. <b>Conclusion:</b> Deltoid repair and reconstruction were comparable in returning the ankles to an intact state and conferring stability. These results suggest that in cases with insufficient or unstable deltoid ligament where repair is not possible, reconstruction has the potential to be a reliable alternative, but further studies are warranted to understand all advantages/disadvantages.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6313005"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peroxide-Free Titanium Dioxide Nanoparticle-Based Photocatalytic Bleaching: In Vitro Study on Bovine Teeth.","authors":"Tainah Oliveira Rifane, Isabel Silva Nascimento, Suely Cristina Aragão Veras Santos, Lucca Reis Mesquita, Diego Martins de Paula, Victor P Feitosa","doi":"10.1155/bmri/9311501","DOIUrl":"10.1155/bmri/9311501","url":null,"abstract":"<p><p><b>Objective:</b> This study is aimed at assessing the bleaching efficacy of titanium dioxide (TiO2) nanoparticle suspensions at different concentrations and exposure times for photocatalysis and evaluating their potential as a peroxide-free alternative to carbamide peroxide in dental bleaching. <b>Materials and Methods:</b> Sixty bovine incisors were stained with black tea and treated with different bleaching protocols: (1) control (16% carbamide peroxide), (2) TiO₂-50 wt% for 10 min (TiO₂ 50/10), (3) TiO₂-50 wt% for 50 min (TiO₂ 50/50), (4) TiO₂-10 wt% for 50 min (TiO2 10/50), and (5) TiO₂-10 wt% for 10 min (TiO₂ 10/10). The agents were exposed to UV light (395 nm) for photocatalysis before application. All treatments were applied daily for 2 h over 21 days. Color change (Δ<i>E</i>00) was measured using a digital spectrophotometer (VITA Easyshade V), and pH was assessed using pH strips. <b>Results:</b> The data were analyzed using one-way ANOVA and Tukey's test. TiO₂ 50/50 showed significantly superior bleaching effects compared to all other groups (<i>p</i> < 0.001). TiO₂ 10/50 and TiO₂ 10/10 demonstrated intermediate bleaching results, with no significant difference between them (<i>p</i> = 0.0875). The control and TiO₂ 50/10 groups exhibited the lowest color variation (<i>p</i> = 0.102). All groups maintained a pH of 6 before and after 2 h of the at-home bleaching protocol. <b>Conclusion:</b> TiO₂ nanoparticle suspensions may be a viable peroxide-free alternative for dental bleaching, particularly at higher concentrations and longer photocatalysis exposure times.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9311501"},"PeriodicalIF":2.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Access to ART Services: Lived Experiences and Coping Strategies of HIV-Positive Persons With Visual Impairment in Lira District, Northern Uganda.","authors":"Gloria Ketty Acila, Noeline Aju Ijorea, Amir Kabunga, Mercy Joy Angom, Sandra Talemwa, Patrick Ayer, Anna Grace Auma","doi":"10.1155/bmri/1903886","DOIUrl":"10.1155/bmri/1903886","url":null,"abstract":"<p><p><b>Background:</b> Despite progress in the HIV/AIDS response, marginalized populations persistently face unique challenges in accessing essential healthcare services, including antiretroviral therapy. The aim of this qualitative study is to explore and understand the lived experiences of individuals living with HIV and visual impairment in Lira District, Northern Uganda, specifically focusing on their access to antiretroviral therapy services. Additionally, the study is aimed at identifying and analyzing the coping strategies employed by this population in navigating the intersectionality of HIV and visual impairment. <b>Methods:</b> This qualitative study, conducted in Lira District, Northern Uganda, employed a descriptive phenomenological design. Thirty in-depth interviews were conducted at Lira Regional Referral Hospital, focusing on individuals living with both HIV and visual impairment. Data collection involved a semistructured interview guide, addressing key issues derived from a literature review. Thematic analysis was used for data analysis, guided by Braun and Clarke's framework. <b>Results:</b> Participants (<i>N</i> = 30) exhibited diverse sociodemographic characteristics, with ages ranging from 19 to 68 years. A number of themes emerged during data analysis: individuals developing visual impairment before and after ART initiation. Emotional challenges, strained relationships, and perceived burdenship were common themes among participants. Limited understanding of the cause of sight loss and a heightened perceived risk of HIV infection were evident. Challenges in accessing ART services included transportation difficulties, negative attitudes from healthcare workers, and a lack of trust in community drug distribution points. Participants employed various coping strategies, including prayers/religion, reliance on social support networks, denial, acceptance, and community rehabilitation. Positive religious beliefs offered hope, while social support played a crucial role in adaptation. Community rehabilitation and support were highlighted as instrumental in aiding coping. <b>Conclusion:</b> Despite awareness about the risk of HIV infection, significant barriers persist in accessing ART services for persons with visual impairment. Coping strategies underscore the importance of addressing psychosocial aspects. Tailored interventions, education, and policy changes are imperative to enhance inclusivity and accessibility of healthcare services for this vulnerable population in Uganda.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"1903886"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Neutrophil CD11b Compared to Neutrophil CD64 as an Early Diagnostic, Monitoring, and Prognostic Sepsis Marker in Neonatal ICUs: Case-Control-Methodological Study.","authors":"Heba E Hashem, Wafaa O Ahmed, Safeya H Hassan","doi":"10.1155/bmri/7206112","DOIUrl":"https://doi.org/10.1155/bmri/7206112","url":null,"abstract":"<p><p><b>Background:</b> Early diagnosis and treatment of neonatal sepsis are crucial to cut off its major medical consequences: lifelong morbidities, neurodevelopmental disabilities, and a high number of neonatal mortalities. <b>Aim of the Work:</b> This study is aimed at determining the diagnostic and prognostic performance of CD11b as a sepsis biomarker for detecting neonatal sepsis at early stages compared to nCD64 and the other conventional sepsis parameters. <b>Methods:</b> Two hundred eleven neonates were enrolled from three Egyptian neonatal ICUs (NICUs), and they were classified into two main groups: the control group (<i>n</i> = 101) and the sepsis group (<i>n</i> = 110). Enrolled neonates were subjected to full sepsis screening, including complete blood count (CBC), C-reactive protein (CRP), blood cultures, and flow cytometry analysis for both CD64 and CD11b on the neutrophil surface (results represented as a percentage (percent) and mean fluorescent intensity (MFI) units for either biomarker). <b>Results:</b> nCD64% (median = 44.15%) was significantly enhanced in the sepsis group compared to the controls (median = 25%), achieving 90.8% specificity, 92.8% sensitivity, and AUC = 0.894, respectively. CD64 MFI and CD11b MFI could differentiate between sepsis and control groups but with low undesirable diagnostic performance (sensitivity: 72.5% and 59.1%; specificity: 54.4% and 69.4%; AUC: 0.634 and 0.144, respectively). CD11b% could not discriminate between sepsis and control neonates (sensitivity and specificity of 31.8% and 73.6%, respectively) with an AUC of 0.405. hs-CRP had moderate diagnostic performance, achieving sensitivity and specificity of 69% and 78.15%, respectively, and AUC = 0.586. ROC analysis showed that combined hs-CRP and CD64% results had the highest sensitivity and specificity in the current study, being 93.9% and 97.2%, with AUC = 0.938, respectively. <b>Conclusion:</b> CD64%, CD64 MFI, CD11b MFI, and hs-CRP are increased in neonates with sepsis comparable to the controls. CD64% has a superior diagnostic performance comparable to nCD11b and hs-CRP. Combined nCD64 with hs-CRP measurement can provide rapid and accurate diagnostic modality for sepsis diagnosis in correlation with the patient's clinical condition and context with the results of other hematological indices; neutrophil CD64 can be routinely applicable in NICUs for better sepsis management. It is statistically evident that nCD11b is less ideal compared to nCD64 as a diagnostic, prognostic, or monitoring sepsis marker.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"7206112"},"PeriodicalIF":2.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational Age-Specific Prevalence of Preeclampsia Among Pregnant Women in Ghana: A Hospital-Based Retrospective Observational Study.","authors":"Wina Ivy Ofori Boadu, Enoch Odame Anto, Akua Benewaah Asamoah, Ezekiel Ansah, Godfred Yawson Scott, Emmanuel Ekow Korsah, Joseph Frimpong, Valentine Christian Kodzo Tsatsu Tamakloe, Michael Nyantakyi, Agartha Odame Anto, Emmanuel Timmy Donkoh, Kwame Ofori Boadu, Richard Vikpebah Duneeh, Frederick Ayensu, Christian Obirikorang","doi":"10.1155/bmri/4728838","DOIUrl":"https://doi.org/10.1155/bmri/4728838","url":null,"abstract":"<p><p><b>Background:</b> Preeclampsia is responsible for a significant rate of maternal morbidity and mortality worldwide. Gestational age as a risk factor has a significant impact on fetal outcomes in pregnancies affected by preeclampsia. However, to our knowledge, no study has identified the gestational age-specific prevalence of preeclampsia in Ghana. Thus, this study ascertained the gestational age-specific prevalence of preeclampsia as well as its associated factors. <b>Methods:</b> A hospital-based retrospective observational study was conducted by reviewing data collected from the maternal birth register on 619 pregnant women who delivered at the facility from 1st January 2021 to 31st December 2021. <b>Results:</b> Out of 619 pregnant women, the overall prevalence of preeclampsia among the study participants was 10.5% whereas 80.5% were normotensive pregnant women. The gestational age-specific prevalence of preeclampsia was 2.3%, 2.1%, 4.0%, 1.6%, and 0.5% at < 37 weeks, 37-38 weeks, 39-40 weeks, 41 weeks, and ≥ 42 weeks, respectively. Most of the mothers who had preeclampsia were within the age group of 30-39 years (40, 61.5%), had informal education (41, 64.1%), and were multigravida (40, 61.5%). Age group 30-39 years (aOR = 2.49, 95% CI (1.25-4.96), <i>p</i> = 0.0090), C/S (aOR = 2.83, 95% CI (1.46-5.50), <i>p</i> = 0.0020), and gestational age category < 37 weeks (aOR = 0.24, 95% CI (0.07-0.78), <i>p</i> ≤ 0.0140) and 37-38 weeks (aOR = 0.23, 95% CI (0.08-0.66), <i>p</i> = 0.0060) were the independent predictors of preeclampsia, respectively, with head circumference < 33 cm (aOR = 2.09, 95% CI (1.00-4.37), <i>p</i> = 0.0490) as the independent complication associated with it. <b>Conclusions:</b> Gestational age-specific prevalence of preeclampsia is high at full-term (39-40 weeks) gestation. Independent risk factors for preeclampsia included maternal age (30-39 years), gestational age (< 37 weeks), and previous caesarean section. Babies of women with preeclampsia are likely to have a small head circumference.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"4728838"},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of an Extracellular Matrix-Related Risk Model to Analyze the Correlation Between Glioblastoma and Tumor Immunity.","authors":"Jian Li, Hong Pan, Yangyang Wang, Haixin Chen, Zhaopeng Song, Zheng Wang, Jinxing Li","doi":"10.1155/bmri/2004975","DOIUrl":"https://doi.org/10.1155/bmri/2004975","url":null,"abstract":"<p><p><b>Background:</b> Abnormalities in the extracellular matrix (ECM) have been shown to play a crucial role in promoting the formation, progression, and metastasis of glioblastoma multiforme (GBM). Therefore, our study is aimed at constructing a prognostic model based on ECM-related factors, to predict the prognosis of patients with GBM. <b>Methods:</b> We employed single-sample gene set enrichment analysis (ssGSEA) to establish the ECM index of GBM. The identification of candidate genes was achieved by differential analysis conducted between ECM index groups, as well as through the utilization of weighted gene coexpression network analysis (WGCNA) and gene enrichment analysis. We conducted functional validation to confirm the significance of five biomarkers that were tested in the U251 cell line. The screening of prognostic genes was conducted using least absolute shrinkage and selection operator (LASSO) and univariate Cox analysis. The predictive relevance of the risk score model was assessed by using Kaplan-Meier (KM) survival curves in both The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) cohorts. In addition, we conducted immunological studies, created and verified a nomogram, and constructed a network involving long noncoding RNA (lncRNA), microRNA (miRNA), and messenger RNA (mRNA). <b>Results:</b> We identified 45 candidate genes by overlapping the 59 WGCNA core genes with the 855 differentially expressed genes (DEGs) between ECM index groups. These candidate genes were significantly enriched in 254 biological processes (BPs), 18 cellular components (CCs), 27 molecular functions (MFs), and 11 KEGG pathways. We identified a prognostic ECM-related five-gene signature using these candidate genes and constructed a risk model. Furthermore, we generated a nomogram model with excellent predictive ability. We also found significant differences between risk groups in six cell types and 29 immune checkpoints. Finally, we constructed a lncRNA-miRNA-mRNA network consisting of 27 lncRNAs, 73 miRNAs, and 5 model mRNAs. <b>Conclusion:</b> Our study developed a prognostic model based on the ECM-related five-gene signature, which can serve as a valuable reference for the treatment and prophetic prediction of GBM.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"2004975"},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Intensity Interval Training Enhances the Positive Effect of Pentoxifylline on Lipid Profile and Inflammatory Markers in an Endometriosis Animal Model.","authors":"Zahra Salehpoor, Maryam Koushkie Jahromi","doi":"10.1155/bmri/6742953","DOIUrl":"https://doi.org/10.1155/bmri/6742953","url":null,"abstract":"<p><p><b>Background:</b> The relationship between endometriosis and cardiovascular disease (CVD) is well established. However, the effects of various exercise training modalities and the anti-inflammatory effects of pentoxifylline (PTX) remain inadequately understood. This investigation is aimed at evaluating the effects of PTX, both independently and in conjunction with high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT), on lipid and inflammatory markers including triglycerides (TGs), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and C-reactive protein (CRP) in a rat model of endometriosis. <b>Materials and Methods:</b> Sprague-Dawley's rats were divided into two primary groups: the healthy control group that received no intervention and the induced endometriosis group. Endometriosis was surgically induced in rats, and those with confirmed endometriotic lesions were further categorized into six groups: control, MICT, drug of PTX (D), MICT+D, HIIT, and HIIT+D. Two weeks after laparotomy, PTX consumption and exercise training were performed for 8 weeks. PTX was administered orally at 100 mg/kg/day. MICT and HIIT sessions were conducted 5 days per week, with MICT beginning at 55% of maximum capacity for 31 min in the first week and progressing to 70% of maximum capacity for 46 min by the eighth week. HIIT sessions consisted of 2 min of running followed by 1 min of passive rest at 85% of maximum capacity, starting with seven intervals in the first week and increasing to twelve by the end of the eighth week. The macroscopic size of endometriosis lesions was measured, and cardiovascular risk factors, including hs-CRP, TC, TG, HDL-C, and LDL-C, were assessed in serum samples. <b>Results:</b> The induction of endometriosis was associated with elevated cardiovascular risk factors, including hs-CRP, TC, and TG. HIIT+D significantly decreased lesion volume (<i>p</i> < 0.0001, 95%confidence interval (CI) = 57.239-94.718), hs-CRP (<i>p</i> = 0.049, CI = -54.083 to - 29.478), TC (<i>p</i> = 0.045, CI = -38.607 to - 25.392), and TG (<i>p</i> = 0.042, CI = 25.531-55.801). PTX significantly decreased lesion volume (<i>p</i> < 0.0001, CI =34.709-73.919) and TC (<i>p</i> = 0.016, CI = -45.153 to - 30.179). <b>Conclusion:</b> All interventions except MICT reduced lesion volume, whereas only HIIT+PTX and PTX, in the order of importance, improved some cardiovascular risk indices in the rat model of endometriosis.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6742953"},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}