BioMed Research International最新文献

{"title":"Applications and Prospects of Single-Cell RNA Sequencing and Spatial Transcriptomics in Cervical Cancer.","authors":"Yifu Wang, Li Yang, Yunzhi Liu, Huangrong Ma, Miaoying Cai, Chunyu Liang, Li Zhang, Zezhuo Su, Zhiyuan Xu","doi":"10.1155/bmri/1532745","DOIUrl":"10.1155/bmri/1532745","url":null,"abstract":"<p><p>Cervical cancer (CC) is the fourth commonest malignant tumor among women worldwide and is characterized by high heterogeneity and a complex ecosystem. A comprehensive understanding of the heterogeneity of tumors and the tumor microenvironment (TME) is crucial for effective CC management. Single-cell RNA sequencing (scRNA-seq) is a powerful tool that can be employed to unveil the heterogeneity of tumors and the TME, as well as to elucidate the evolutionary trajectories of tumors. Spatial transcriptomics (ST) technology, on the other hand, can address the complexity and diversity of the spatial microenvironment of tumors, thereby compensating for the limitations of scRNA-seq. As emerging technologies, both scRNA-seq and ST are increasingly being utilized in CC research. In this review, we summarized the latest advancements in scRNA-seq and ST for CC, with a focus on investigating tumor heterogeneity, the TME, tumor evolutionary trajectories, treatment resistance mechanisms, and potential therapeutic targets. These insights collectively contribute to the development of more effective treatment and prevention strategies for CC.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"1532745"},"PeriodicalIF":2.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improves Cartilage Repair in a Rabbit Model\".","authors":"","doi":"10.1155/2025/9780636","DOIUrl":"https://doi.org/10.1155/2025/9780636","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2021/6380141.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9780636"},"PeriodicalIF":2.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance Profiling of Predominant Non-<i>E. coli</i> Enterobacteriaceae Isolated From Humans, Food Animals, and the Environment in the Fako Division of Cameroon.","authors":"Clovis Elah Ndialle, Mildred Mbom Nyincho, Matil Eyong, Derick Lekealem Nkwetta, Manuel Ritter, Patrick A Njukeng, Samuel Wanji, Jane-Francis T K Akoachere","doi":"10.1155/bmri/3947539","DOIUrl":"10.1155/bmri/3947539","url":null,"abstract":"<p><p>The impact of the current global rising resistance of Enterobacteriaceae to antibiotic agents is of great concern. Detecting and monitoring resistance in these pathogens in humans, animals, and the environment and taking appropriate actions based on results obtained are indispensable to reverse this trend. This study is aimed at contributing to the fight against resistance of predominant non-<i>Escherichia coli</i> Enterobacteriaceae in the Fako Division of Cameroon through a one-health approach. Freshly collected human feces, rectal swabs from pigs, cloacal swabs from chicken, cow intestinal content, and environmental samples were cultured. Isolates were identified using API 20E. Predominant non-<i>E. coli</i> isolates (<i>Enterobacter</i> spp., 65.0%; <i>Salmonella</i> spp., 11%; and <i>Citrobacter</i> spp., 9.9%) were confirmed by polymerase chain reaction (PCR). Antibiotic susceptibility profiles of these isolates were determined by the Kirby-Bauer disc diffusion technique, while the resistant genes were detected by PCR. The quinolones (norfloxacin, 94.7%, and ofloxacin, 91.2%), carbapenem (imipenem, 96%), aminoglycoside (amikacin, 95.5%), and chloramphenicol (91.3%) were the most active drugs. Penicillins (amoxicillin, 24.7%; ampicillin, 21.2%; and amoxicillin-clavulanic acid, 19.9%) were the most inactive drugs. However, isolates showed the highest rate of intermediate susceptibility (48.6%) to cefepime. Out of the 226 isolates, 214 (94.7%) showed resistance to at least one antibiotic agent. Multidrug resistance was found in 54 (25.2%) of the isolates. The predominant antibiotypes were AX^R AM^R AMC^R (25, 11.1%), AX^R AM^R AMC^R AZM^R (18, 8.4%), CAZ^R AX^R AM^R AMC^R (12, 5.3%), and AX^R AMC^R AZM^R (7, 3.1%). Isolates with these antibiotypes were from various sources and predominant genera. Plasmid-mediated quinolone resistance (PMQR) genes (<i>acrA</i>, <i>acrB</i>, <i>qepA</i>, and <i>aac(6</i>⁣' <i>)-ib-cr</i>) were detected in 97.8% (44/45) of isolates that showed resistance to at least one quinolone antibiotic, while the beta-lactamase genes, <i>blaCMY-2</i> and <i>blaCTX-M-1</i>, were detected in 7.9% (5/63) and 22.0% (14/63), respectively, in isolates that showed resistance to cephalosporins. These isolates carrying these genes were from humans, food animals, and the environment. Of the 45 isolates, a total of 40 (88.9%) carried two or more PMQR genes, while 2 (0.6%) carried both <i>bla</i> genes (cocarriage). Five (17.9%) isolates out of the 28 screened for PMQR and beta-lactamase genes were positive for both sets of genes. Resistance to antibiotics was high with strains of the different genera carrying PMQR and beta-lactamase resistance genes circulating in humans, food animals, and the environment in the Fako Division of Cameroon.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"3947539"},"PeriodicalIF":2.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12208769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Artificial Intelligence in Orthognathic Surgery: A Scoping Review.","authors":"Saeed Reza Motamedian, Sadra Mohaghegh, Maral Niazmand, Hossein Mohammad-Rahimi, Nima Ahmadi, Mina Yaseri, Helia Sadat Haeri Boroojeni","doi":"10.1155/bmri/8284581","DOIUrl":"10.1155/bmri/8284581","url":null,"abstract":"<p><p><b>Objective:</b> This study was aimed at reviewing the application of different artificial intelligent algorithms used in different phases of orthognathic surgeries, which include diagnosis, treatment planning, soft tissue prediction, outcome evaluation, and complication assessment. This aimed to update clinicians on this technology to integrate it into their decision-making, in addition to being aware of its challenges and potential areas for further assessment. <b>Materials and Methods:</b> Electronic search was done in PubMed, Scopus, Embase, and Cochrane databases. Studies that reported the application of artificial intelligence (AI) in different aspects of orthognathic surgery were included. <b>Results:</b> From 656 studies, a total of 29 articles met the inclusion criteria and were used to categorize the application of AI as follows: (1) Diagnosis in which studies showed the sensitivity of 75%-95.5% for specifying the need for orthognathic surgery; (2) treatment planning in which AI was used for osteotomy design and bony reference point determination with 3.99-4.73 mm of error; (3) soft tissue prediction in which AI models showed a success rate of 64.3%-100%; (4) outcome evaluation in which AI was used to assess the impact of surgery on asymmetry, facial attractiveness, and esthetic improvements quantitatively; and (5) complication assessment with an accuracy of 98.7% for predicting postsurgical systemic infection and 7.4 mL of error for blood loss. <b>Conclusion:</b> AI can be potentially considered as a proper alternative to conventional approaches to fasten the procedure related to orthognathic surgery and with comparable accuracy to conventional methods.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"8284581"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Identification of Kinesin Family Member 2A (KIF2A) as a Promising Therapeutic Target for Osteosarcoma.","authors":"BioMed Research International","doi":"10.1155/bmri/9808573","DOIUrl":"10.1155/bmri/9808573","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2020/7102757.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9808573"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: HIF-1-miR-219-SMC4 Regulatory Pathway Promoting Proliferation and Migration of HCC under Hypoxic Condition.","authors":"BioMed Research International","doi":"10.1155/bmri/9798545","DOIUrl":"10.1155/bmri/9798545","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2019/8983704.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9798545"},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: \"Platelet-Rich Plasma Combined with Alendronate Reduces Pain and Inflammation in Induced Osteoarthritis in Rats by Inhibiting the Nuclear Factor-Kappa B Signaling Pathway\".","authors":"BioMed Research International","doi":"10.1155/bmri/9840675","DOIUrl":"https://doi.org/10.1155/bmri/9840675","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2020/8070295.].</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"9840675"},"PeriodicalIF":2.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12173551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular Cell-Conditioned Chitosan Thermosensitive Hydrogel as an Excellent Substrate for Culture and Proliferation of Spermatogonia Cells.","authors":"Monireh Mahmoodi, Mahdieh Gholipour-Malekabadi","doi":"10.1155/bmri/6693200","DOIUrl":"10.1155/bmri/6693200","url":null,"abstract":"<p><p>Successful in vitro transplantation of spermatogonia cells (SCs) requires effective culture systems for the proliferation of SCs. The natural extracellular matrix (ECM) creates a suitable microenvironment for stem cell culture. In the present study, a temperature-sensitive hydrogel scaffold was fabricated from chitosan (TCTS), a biocompatible polysaccharide with the ability to turn into gel using <i>β</i>-glycerophosphate as a crosslinker at 37°C. Then, the surface of the hydrogel scaffold was covered with the ECM secreted from testicular cells (TC-TCTS) to provide an excellent substrate for the adhesion and proliferation of SCs toward sperm-producing cells. The synthesized scaffold had a uniform morphology with high biocompatibility and adhesion for both testicular cells and SCs. SEM micrographs, DNA content, and DAPI confirmed the presence of ECM and successful removal of the TC from the hydrogel after decellularization. TC-TCTS showed a higher rate of water absorption and weight loss (around 80%) compared to TCTS. The survival of SCs seeded on the TC-TCTS hydrogel showed a significant increase compared with TCTS. The SCs cultured on TC-TCTS were much more elongated and had a greater tendency to stick to the surface of the hydrogel compared with TC-TCTS. Molecular studies showed a significant increase in the expression of proliferation-associated genes (around 4.5-fold for <i>id4</i> and around 7-fold for <i>plzf</i>) in SCs cultured on the TC-TCTS compared with TCTS and control. Our findings confirmed that the TC-TCTS can provide an excellent microenvironment biomimicking the natural ECM of seminiferous tubules for adhesion and proliferation of SCs and possible production of sperm in vitro in the future investigations.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"6693200"},"PeriodicalIF":2.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Profile of Diabetic Wounds at Laquintinie Hospital Douala and the Antimicrobial Potential of Spiced Fish (<i>Fontitrygon margarita</i>) Liver Oil Against Multiresistant Isolates.","authors":"Estelle Djeukoua Djeuya, Joseph Paul Marius Koualiagnigni, Fabrice Fabien Dongho Dongmo, Sammuel Raymond Tchabong, Sorelle Daina Nanhou Nkepndep, Boris Simo Noutsa, Alix G T Tchoupe, Josiane Essola, Rebecca Madeleine Ebelle Etame, Modeste Lambert Sameza","doi":"10.1155/bmri/3158942","DOIUrl":"10.1155/bmri/3158942","url":null,"abstract":"<p><p>This study investigates the bacteriological profile, antibiotic susceptibility, and potential natural treatment for diabetic wound infections at Laquintinie Hospital in Douala, Cameroon. Over 2 months (June to July 2024), 75 diabetic patients with wounds were analyzed, revealing a 73.08% infection rate. The most common bacterial isolates were <i>Staphylococcus aureus</i> (54.38%), <i>Klebsiella pneumoniae</i> (40.35%), and <i>Escherichia coli</i> (29.82%), with significant antibiotic resistance observed. Imipenem (82.65% sensitivity) and gentamicin (72.88% sensitivity) were the most effective antibiotics, while amoxicillin-clavulanic acid showed the highest resistance rate (46.94%). This study also evaluated the antibacterial and antioxidant efficacy of <i>Fontitrygon margarita</i> liver oil, particularly when extracted with spices such as <i>Monodora myristica</i> and <i>Zingiber officinale</i>. The oil demonstrated significant antibacterial activity against multiresistant bacterial isolates, with minimum inhibitory concentrations ranging from 16 to 128 mg/mL. Incorporating spices during extraction enhanced the oil's antibacterial activity, showing synergistic effects in 58.33% of cases. The oil also exhibited strong antioxidant properties, with improvements noted in DPPH, ABTS, and FRAP assays when spices were added, highlighting the highest efficacy with <i>M. myristica</i> and <i>Z. officinale</i> extracts. These findings suggest that <i>F. margarita</i> liver oil, especially when combined with spices, could serve as a valuable natural alternative for managing diabetic wound infections, addressing the challenges posed by antibiotic resistance and oxidative stress. Further research and clinical trials are recommended to validate these results and explore their practical applications.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"3158942"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of PANoptosis-Related Genes in Predicting Breast Cancer Survival and Immune Prospect.","authors":"Yuxi Zhang, Zheming Liu, Yixuan Zhang, Xue Zhang, Yi Yao, Chi Zhang","doi":"10.1155/bmri/3423698","DOIUrl":"10.1155/bmri/3423698","url":null,"abstract":"<p><p><b>Background:</b> The function of PANoptosis in breast cancer (BC) remains indistinct. We constructed a nomogram model to predict the prognosis of BC to identify high-risk patients and help them receive more accurate treatment. <b>Method:</b> We used Cox regression and least absolute shrinkage and selection operator (LASSO) algorithm to select PANoptosis-related genes (PRGs) and calculated the PANoptosis-related score (PRS) by LASSO coefficient. Through functional enrichment, somatic mutation, and tumor microenvironment (TME) analysis, we completed the identification of PANoptosis-related immune cells and difference analysis of drug sensitivity and then verified key genes by performing survival analysis. <b>Results:</b> Patients were divided into low- and high-risk cohorts depending on PRS, and the negative association between risk scores and overall survival was disclosed. Analysis showed that differentially expressed genes in the two risk cohorts were mainly concentrated among pathways related to the immune system. Moreover, we detected distinguished differences in immune checkpoints, tumor mutation load, and TME in the two cohorts. Furthermore, KLHDC7B, GNG8, IGKV1OR2-108, and IGHD were identified as key genes. We also found that hub genes were highly expressed in tumor tissues, while B cells, CD4+, and CD8+ T cells pretended to be positive among the hub gene-negative cohort. Prognosis analysis showed that pivotal genes had adverse effects on survival over time. <b>Conclusion:</b> We built a precise prediction model based on risk scores and proved the significance of PRGs in BC TME and medicine sensitivity regulation, providing key perception for subsequent molecular mechanism studies and contributing to more personalized treatment decisions in clinical practice.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2025 ","pages":"3423698"},"PeriodicalIF":2.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}