ACS Biomaterials Science & Engineering最新文献

{"title":"Recent Progress in the Strategies and Applications of Electrospinning Electroactive Tissue Engineering Scaffolds.","authors":"Yixun Li, Xinyu Li, Zhiwei Liu, Yuehua Wang, Tifeng Jiao","doi":"10.1021/acsbiomaterials.5c00142","DOIUrl":"10.1021/acsbiomaterials.5c00142","url":null,"abstract":"<p><p>Conductive biomaterials not only have appropriate conductivity but also usually have good antibacterial properties and photothermal effects, so they are widely used in tissue engineering scaffolds. Conductive biomaterials can conduct endogenous or exogenous electrical signals, thus affecting the growth, migration, infiltration, and differentiation of cells. An electrospun nanofiber is an ideal kind of conductive substance carrier that can mimic the extracellular matrix (ECM) to further promote cell growth and migration. In this Review, we summarize the application of electrospinning electroactive tissue engineering scaffolds, discuss the advantages and disadvantages of various electrospinning methods, organize the characteristics of commonly used conductive biomaterials such as polyaniline (PANI), polypyrrole (PPy), poly(3,4-ethylene dioxythiophene) (PEDOT), carbon-based nanomaterials, and MXenes and their application in the tissue engineering field, and finally propose the application prospects and future of tissue engineering with conductive biomaterials.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3182-3200"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptations of Gram-Negative and Gram-Positive Probiotic Bacteria in Engineered Living Materials.","authors":"Varun Sai Tadimarri, Tanya Amit Tyagi, Cao Nguyen Duong, Sari Rasheed, Rolf Müller, Shrikrishnan Sankaran","doi":"10.1021/acsbiomaterials.5c00325","DOIUrl":"10.1021/acsbiomaterials.5c00325","url":null,"abstract":"<p><p>Encapsulation of microbes in natural or synthetic matrices is a key aspect of engineered living materials, although the influence of such confinement on microbial behavior is poorly understood. A few recent studies have shown that the spatial confinement and mechanical properties of the encapsulating material significantly influence microbial behavior, including growth, metabolism, and gene expression. However, comparative studies within different bacterial species under identical confinement conditions are limited. In this study, Gram-negative <i>Escherichia coli</i> Nissle 1917 and Gram-positive <i>Lactiplantibacillus plantarum</i> WCFS1 were encapsulated in hydrogel matrices, and their growth, metabolic activity, and recombinant gene expression were examined under varying degrees of hydrogel stiffness, achieved by adjusting the polymer concentration and chemical cross-linking. Both bacteria grow from single cells into confined colonies, but more interestingly, in <i>E. coli</i> gels, mechanical properties influenced colony growth, size, and morphology, whereas this did not occur in <i>L. plantarum</i> gels. However, with both bacteria, increased matrix stiffness led to higher levels of recombinant protein production within the colonies. By measuring metabolic heat from the bacterial gels using the isothermal microcalorimetry technique, it was inferred that <i>E. coli</i> adapts to the mechanical restrictions through multiple metabolic transitions and is significantly affected by the different hydrogel properties. Contrastingly, both of these aspects were not observed with <i>L. plantarum</i>. These results revealed that despite both bacteria being gut-adapted probiotics with similar geometries, mechanical confinement affects them considerably differently. The weaker influence of matrix stiffness on <i>L. plantarum</i> is attributed to its slower growth and thicker cell wall, possibly enabling the generation of higher turgor pressures to overcome restrictive forces under confinement. By providing fundamental insights into the interplay between mechanical forces and bacterial physiology, this work advances our understanding of how matrix properties shape bacterial behavior. The implications of these findings will aid the design of engineered living materials for therapeutic applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3773-3784"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Shear and Extensional Stresses on Cells: Investigation in a Spiral Microchannel and Contraction-Expansion Arrays.","authors":"Thammawit Suwannaphan, Ampol Kamnerdsook, Suramate Chalermwisutkul, Boonchai Techaumnat, Nattapol Damrongplasit, Bhawat Traipattanakul, Surasak Kasetsirikul, Alongkorn Pimpin","doi":"10.1021/acsbiomaterials.5c00555","DOIUrl":"10.1021/acsbiomaterials.5c00555","url":null,"abstract":"<p><p>In recent decades, inertial microfluidic devices have been widely used for cell separation. However, these techniques inevitably exert mechanical stresses, causing cell damage and death during the separation process. This remains a significant challenge for their biological and clinical applications. Despite extensive research on cell separation, the effects of mechanical stresses on cells in microfluidic separation have remained insufficiently explored. This review focuses on the effects of mechanical stresses on cells, particularly in spiral microchannels and contraction-expansion arrays (Contraction and Expansion Arrays (CEAs)). We derived the approximated magnitude of shear stress in a spiral microchannel, extensional stress in CEAs and conventional methods, along with exposure time in a single map to illustrate cell damage and operational zones. Finally, this review serves as a practical guideline to help readers in evaluating stress damages, enabling the effective selection of appropriate techniques that optimize cell viability and separation efficiency for biological and clinical applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3249-3261"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Nael Berri,&nbsp;Sandhya Moise,&nbsp;Antonios Keirouz,&nbsp;Andrew Jennings,&nbsp;Bernardo Castro-Dominguez and Hannah S. Leese*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsbiomaterials.5c00146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144358019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Pd-Loaded Hf-Based Metal-Organic Framework as a Dual-Modal Contrast Agent for Photoacoustic Imaging and Computed Tomography.","authors":"Yen-Chang Chen, Yu-Sheng Yu, Yu-Kang Wang, R K Rakesh Kumar, Cho-Yin Lee, Cheng-Hsin Chuang, Lun-De Liao, Kevin C-W Wu","doi":"10.1021/acsbiomaterials.5c00169","DOIUrl":"10.1021/acsbiomaterials.5c00169","url":null,"abstract":"<p><p>Noninvasive cancer imaging significantly improves diagnostics by providing comprehensive structural and functional information about tumors. Herein, we explored palladium nanoparticles loaded hafnium-based metal-organic framework (MOF) (Hf-EDB), i.e., Pd@Hf-EDB as an efficient dual modal contrast agent for computed tomography (CT) and photoacoustic imaging (PAI). The synergistic collaborations between (i) high-Z element Hf-based MOF with superior X-rays absorbing capabilities, (ii) H₂EDB linkers with special π-donation and π-acceptor characteristics capable of strongly anchoring noble metals, and (iii) Pd nanoparticles with broad absorption in the UV to near-infrared (NIR) regions due to strong interband transition are ideal for implementation in CT and PAI. The successful synthesis of Pd@Hf-EDB nanoparticles was confirmed through morphology, crystallinity, and compositional characterizations using X-ray diffraction, SEM, TEM, DLS, and EDS. Soft X-ray tomography verified cellular uptake via phagocytosis of Pd@Hf-EDB by BxPC-3 tumor cells. In-vitro experiments revealed superior CT imaging performance of Pd@Hf-EDB over traditional molecular contrast agents like Iohexol. Broad absorption range in the UV-vis/NIR regions and superior PAI capabilities of Pd@Hf-EDB relative to gold nanorods are reported. Furthermore, the in vivo xenograft model demonstrated significant contrast enhancements near the tumor, highlighting the excellent PAI and CT capabilities of the synthesized Pd@Hf-EDB.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3634-3648"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transition Metal Dichalcogenides in Biomedical Devices and Biosensors: A New Frontier for Precision Healthcare.","authors":"Eslam M Hamed, Sam F Y Li","doi":"10.1021/acsbiomaterials.4c02465","DOIUrl":"10.1021/acsbiomaterials.4c02465","url":null,"abstract":"<p><p>Transition metal dichalcogenides (TMDs) have emerged as groundbreaking materials in the field of biomedical applications, particularly in the development of biosensors and medical devices. Their unique electronic and optical properties, combined with their tunability and biocompatibility, position TMDs as promising candidates for enhancing early disease detection and enabling personalized medicine. This perspective explores the multifaceted potential of TMDs, highlighting their applications in fluorescence and Raman-based biosensing, wearable and implantable devices, and smart therapeutic systems for targeted treatment. Additionally, we address critical challenges such as regulatory hurdles, long-term stability, and ethical considerations surrounding continuous health monitoring and data privacy. Looking to the future, we envision TMDs playing a vital role in the advancement of precision medicine, facilitating real-time health monitoring and individualized treatments. However, the successful integration of TMDs into clinical practice necessitates interdisciplinary collaboration among materials science, bioengineering, and clinical medicine. By fostering such collaboration, we can fully harness the capabilities of TMDs to revolutionize healthcare, making it more accessible, precise, and personalized for patients worldwide.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3084-3094"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Fabrication of Polycaprolactone-Based Composite Scaffolds for Bone Tissue Engineering: From Chemical Composition to Scaffold Architecture.","authors":"Daniil O Golubchikov, Alexander K Petrov, Vasily A Popkov, Pavel V Evdokimov, Valery I Putlayev","doi":"10.1021/acsbiomaterials.5c00205","DOIUrl":"10.1021/acsbiomaterials.5c00205","url":null,"abstract":"<p><p>Thermoplastic polymer-based materials, which feature essential biological properties and opportunities to implement the cutting-edge additive manufacturing technologies aimed at obtaining high-precision 3D models, have attracted intense interest for porous and bioresorbable bone tissue implants development. Among the wide range of materials, polycaprolactone was found to provide a balance between the biodegradation rate and biocompatibility with various tissues. Recent advances in the fabrication of polymer-polymer and polymer-inorganic composites have opened new ways to improve biological and mechanical outcomes and expanded the range of applications for bone and cartilage restoration, including the development of conductive composites for electrostimulation. While the chemical composition of the manufactured scaffolds played a vital role in their general biological performance and biocompatibility with bone tissue, the micropattern and roughness of the surface were shown to be additional stimuli for stem cell differentiation. More challenges came from the fabrication technique suitable for the proposed scaffold design. Here we summarize the key challenges and advances in fabrication and approaches to the optimization of certain chemical, morphological, or geometrical parameters of polycaprolactone-based scaffolds for bone tissue engineering applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3201-3227"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of Decorin-Mediated Mitochondrial Quality Control in Macrophage Polarization Induced by Titania Nanotube Arrays.","authors":"Meiqi Zhao, Yuqi Zhao, Guangwen Li, Li Zhang, Haochen Wang, Yonglong Hong, Weihua Yu, Wen Song, Yumei Zhang","doi":"10.1021/acsbiomaterials.4c02357","DOIUrl":"10.1021/acsbiomaterials.4c02357","url":null,"abstract":"<p><p>Macrophage polarization critically shapes the local immune microenvironment during bone implant osseointegration and can be modulated by implant surface nanotopography. Unfortunately, the underlying mechanisms still need to be elucidated. Previously our group has confirmed the macrophage polarization rules on titania nanotube arrays (NT) with different diameters. In the present study, we wonder whether mitochondria are involved, considering their significant role in macrophage polarization. The NT surface with a larger diameter (∼100 nm) could induce M1 polarization, accompanied by more active mitochondrial fission and depolarization, as indicated by increased mitochondrial number, reactive oxygen species (ROS) generation, mtDNA/nDNA ratio, and reduced JC-1 aggregation. Further RNA-sequencing revealed the selective upregulation of decorin on nanotube surfaces with larger diameters, and macrophage M1 polarization was diminished after decorin downregulation. As a versatile extracellular matrix molecule, decorin bridges the gap between implant surface nanotopography and mitochondria responses. These findings reveal a mitochondria-centered mechanism whereby implant nanoarchitecture directs immune responses, providing a novel target for designing immunomodulatory biomaterials.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3318-3329"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Smart Nanocomposite Based on Mo₂C MXene for Active Targeted Photothermal-Chemotherapy of Cancer in NIR-II Biowindows.","authors":"Jianfeng Li, Zhihui Xin, Zhiqiang Bai, Jiang Li, Lu Zhao, Yunfeng Bai, Feng Feng","doi":"10.1021/acsbiomaterials.5c00403","DOIUrl":"10.1021/acsbiomaterials.5c00403","url":null,"abstract":"<p><p>As a novel cancer treatment method, photothermal therapy (PTT) is considered an up-and-coming candidate for cancer treatment owing to its low invasiveness and ease of implementation. Nevertheless, single PTT in the first transparency (NIR-I, 750-1000 nm) biowindows is often insufficient to eliminate tumor cells due to light scattering and absorption at the tumor site. Therefore, the rational design of multifunctional nanocomposites for multimodal combination therapies based on PTT is attractive for improving treatment efficacy while reducing drug resistance and adverse reactions. Herein, we report a smart multifunctional nanocomposite DOX-Mo₂C-PAA/Apt-M (DMPM) based on molybdenum carbide (Mo₂C) MXene for active targeted photothermal-chemotherapy in the second transparency (NIR-II, 1000-1350 nm) biowindows. This nanocomposite effectively absorbed light and converted it into heat, achieving a photothermal conversion efficiency of 38.64% under NIR-II laser irradiation. Meanwhile, the DMPM nanocomposite exhibited pH and laser dual-stimuli-triggered doxorubicin (DOX) release in the tumor microenvironment. Furthermore, DMPM could effectively target MCF-7 solid tumors, significantly improving therapeutic efficacy. <i>In vitro</i> and <i>in vivo</i> studies confirmed that DMPM triggered significant cellular killing and tumor eradication without systemic toxicity. Our work not only presents a new approach for multimode cancer treatment but also expands the application of Mo₂C MXene in the biomedical field.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3307-3317"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Bingze Lyu,&nbsp;Haolin Zhou,&nbsp;Huijun Wang,&nbsp;Xuanliang Wang,&nbsp;Ziyue Huang,&nbsp;Yingqi Xu,&nbsp;Zhijie Hu and Huan Peng*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsbiomaterials.5c00218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144373865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}