ACS Biomaterials Science & Engineering最新文献

{"title":"Bioactive Zein–Nondigestible Oligosaccharide Nanofibers Enable Rapid Tissue Regeneration and Antibacterial Defense","authors":"Renugaa Suresh Babu,&nbsp;Piotr Gierlich,&nbsp;Ramakrishnan Swaminathan,&nbsp;Vimalraj Selvaraj,&nbsp;Wendy W. J. Unger,&nbsp;Swathi Sudhakar","doi":"10.1021/acsbiomaterials.6c00314","DOIUrl":"10.1021/acsbiomaterials.6c00314","url":null,"abstract":"<p>Chronic wounds pose a significant clinical challenge due to persistent inflammation, microbial infection, and impaired tissue regeneration. Conventional wound dressings often lack the multifunctionality needed for comprehensive wound management. In this study, we engineered a nanofibrous wound patch composed of zein (ZM) integrated with nondigestible oligosaccharides (ZM-NDOs), such as Chito-oligosaccharides (ZMC), guluronic and mannuronic-rich alginate-oligosaccharides (ZMAGM), and guluronic-rich alginate-oligosaccharides (ZMAG), to address these limitations. Structural and physicochemical analyses (SEM, FTIR, UTM, and water contact angle) confirmed the formation of bead-free, interconnected fibers with enhanced mechanical strength and hydrophilicity. <em>In vitro</em> cytocompatibility assays using HEK 293T cells demonstrated excellent biocompatibility for both ZM and ZM-NDO patches. Notably, ZMC significantly promoted cellular proliferation and migration in wound scratch assays, accelerating wound closure. <em>In vivo</em> evaluation using a Wistar rat excision wound model further substantiated the therapeutic efficacy of the ZM-NDO patches, showing improved collagen deposition, vascularization, and epithelial regeneration, accompanied by upregulated expression of <em>VEGF, TGF-β,</em> and <em>Col-I</em> mRNA. Moreover, the ZMC scaffolds exhibited significant antibacterial activity against <em>Pseudomonas aeruginosa</em> and methicillin-resistant <em>Staphylococcus aureus</em>. Collectively, these findings highlight ZM-NDO, particularly ZMC, as a promising, multifunctional, and scalable platform for advanced wound-healing applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5498–5514"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149072785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extrusion-Stretched Multigrooved Conduits Loaded with Multiwalled Carbon Nanotubes for Efficient Peripheral Nerve Regeneration","authors":"Baolin Wang,&nbsp;Fei Chen,&nbsp;Siyi Zhang,&nbsp;Jialei Mao,&nbsp;Yuanhang Tang,&nbsp;Li-Fang Zhu,&nbsp;Ming-Wei Chang","doi":"10.1021/acsbiomaterials.6c00670","DOIUrl":"10.1021/acsbiomaterials.6c00670","url":null,"abstract":"<p>Nerve guidance conduits (NGCs) represent a promising alternative to autologous nerve transplantation; however, their clinical efficacy remains limited by insufficient structural integrity and suboptimal fascicular guidance. Here, we report a novel extrusion−stretched strategy for fabricating multigrooved NGCs (MNGCs) reinforced with oriented multiwalled carbon nanotubes (MWCNTs). This scalable and controllable method effectively prevents conduit collapse during fabrication and enables precise regulation of conduit geometry, overcoming key limitations of conventional forming approaches. Incorporation of MWCNTs significantly enhanced the mechanical strength and biocompatibility of the conduits, as evidenced by improved Schwann cell (RSC96) viability, density, and pronounced aligned elongation compared with pure polycaprolactone (PCL) conduits. In a rat sciatic nerve defect model, the MWCNT-reinforced multigrooved NGCs (MMNGCs) achieved functional recovery, gastrocnemius muscle regeneration, and axonal myelination comparable to autografts while markedly outperforming single-lumen and groove-only PCL conduits. These results demonstrate that MMNGCs fabricated via the proposed extrusion−stretched strategy constitute a highly effective and clinically competitive platform for peripheral nerve repair.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5583–5596"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix Vesicle Nanostructures Guide Spatially Organized Bone-Like Mineralization in Biomimetic Collagen Scaffolds","authors":"Lucas Fabricio Bahia Nogueira,&nbsp;Yuri Ferreira da Silva,&nbsp;Maryanne Trafani de Melo,&nbsp;Juçara Gastaldi Cominal,&nbsp;Keteryne Rodrigues da Silva,&nbsp;Sandra Yasuyo Fukada,&nbsp;Fausto Almeida,&nbsp;Patrick Perré,&nbsp;Pedro E. D. Augusto,&nbsp;Pietro Ciancaglini,&nbsp;Ana Paula Ramos","doi":"10.1021/acsbiomaterials.6c00914","DOIUrl":"10.1021/acsbiomaterials.6c00914","url":null,"abstract":"<p>Bone formation is tightly regulated by osteoblasts, which secrete extracellular matrix (ECM) components and heterogeneous populations of extracellular vesicles (EVs). Among EVs, matrix vesicles (MVs) are uniquely associated with mineralization and act as bioactive extracellular nanostructures that initiate and spatially direct mineral growth within the ECM. However, how MVs interact with the ECM and how their functions differ from the functions of medium-derived EVs (mEVs) remain unclear. To address this question, we developed a bioinspired collagen-based scaffold designed to mimic the bone ECM organic phase and to recreate MV-mediated mineralization <em>in vitro</em>. Type I collagen scaffolds were slowly concentrated and exposed to NH₃_(g) to induce fibrillogenesis and stabilize supramolecular organization, while 5 wt % κ-carrageenan, a sulfated polysaccharide that functionally emulates glycosaminoglycans, was incorporated to support mineral nucleation. MC3T3-E1 pre-osteoblasts were cultured under osteogenic conditions to induce differentiation and vesicle secretion. Biochemical, microscopic, and spectroscopic analyses revealed that MVs and mEVs exhibit distinct nano-biointerface behaviors, particularly regarding ECM anchoring and the ability to initiate mineral deposition. Furthermore, Raman chemical imaging and X-ray nanotomography demonstrated that MVs not only trigger mineral formation but also direct the spatial organization of phosphate deposition within the ECM at the micro/nanoscale. By distinguishing MV- from mEV-mediated mineralization in a biomimetic ECM system, this study provides new insights into vesicle-guided biomineralization and spatial matrix organization, with implications for skeletal development, pathological calcification, and vesicle-based regenerative strategies.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5627–5645"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-Loaded Porous Titanium Antibacterial Particle Coatings by High-Velocity Embedment into Silicone","authors":"Sandy Liao,&nbsp;Peter C. King,&nbsp;Russell J. Crawford,&nbsp;Elena P. Ivanova,&nbsp;Helmut Thissen,&nbsp;Peter Kingshott","doi":"10.1021/acsbiomaterials.6c00072","DOIUrl":"10.1021/acsbiomaterials.6c00072","url":null,"abstract":"<p>Effective antimicrobial biomaterial surfaces are clinically beneficial as they can prevent infections caused by pathogens that are introduced during surgical procedures. Moreover, they can be advantageous for the long-term performance of medical devices. In the current study, we present an innovative procedure for the manufacture of biocompatible composite surfaces that provide controlled antibiotic release. We utilized titanium particles featuring a porous structure (pTi) as carriers and loaded these with crystalline ciprofloxacin (Cip). We then used these particles to modify the surface of polydimethylsiloxane (PDMS) substrates using cold spray deposition. The resulting surfaces (cipTi-PDMS) were characterized by scanning electron microscopy, energy dispersive X-ray analysis, 3D profilometry, Fourier transform infrared spectroscopy analysis, and water contact angle measurements. The cross-sections of the samples were also analyzed with optical microscopy to determine the embedding layer thickness. The areal density of drug-loaded Ti embedded on the substrate could be enhanced by increasing the cold spray gas temperature or decreasing the nozzle scanning speed. Our results show that the surface modification of PDMS using this method significantly increases the surface roughness and hydrophobicity of samples. The antibacterial efficacy of cipTi-PDMS surfaces against <em><em>Staphylococcus aureus</em></em> was demonstrated using a live/dead staining assay. An agar diffusion test also demonstrated that bacterial growth was effectively prevented for a period of 7 days. Moreover, no cytotoxicity was observed for cipTi-PDMS samples using MG-63 osteoblast-like cells after 7 days of incubation. Our results suggest that the composite surfaces can provide an effective defense against microbial colonization. We expect that this new approach will find applications in a range of polymer-based biomedical device applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5344–5362"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Bone Regeneration Properties of a Hydrogel Loaded with Gallic Acid-Functionalized MgO Nanoparticles to Treat Rheumatoid Arthritis","authors":"Yashika Thakur,&nbsp;Moyna Kalia,&nbsp;Durba Pal,&nbsp;Yashveer Singh","doi":"10.1021/acsbiomaterials.6c01320","DOIUrl":"10.1021/acsbiomaterials.6c01320","url":null,"abstract":"<p>Rheumatoid arthritis (RA), an autoimmune disorder, is characterized by prolonged joint inflammation that eventually damages it. The overexpression of matrix metalloproteinases along with an influx of inflammatory cytokines in the joint, hyperplasticity of fibroblast-like synoviocytes, and increased levels of reactive oxygen species (ROS) results in an acidic microenvironment that irreversibly destroys the cartilage and bone. Methotrexate is used to treat RA, but it is associated with side effects, like nephrotoxicity and immunosuppression, and there is a lack of drug-free strategies to target the progression of disease and tissue regeneration. To treat RA and promote the regeneration of bone tissue, we have developed a drug-free, oxidized chondroitin sulfate-dextran hydrogel loaded with gallic acid-functionalized MgO nanoparticles, which demonstrated promising ROS scavenging and anti-inflammatory activities, as assessed using NO scavenging and reverse-transcription polymerase chain reaction analysis. In addition, the hydrogels were found to promote bone tissue regeneration in cell culture, as determined using the alkaline phosphatase assay and Alizarin red staining along with exhibiting apoptotic potential against hyperplastic fibroblast-like synoviocytes. We report, for the first time, gallic acid-grafted MgO nanoparticles that harness intrinsic antioxidant and apoptotic activity for RA therapy. In summary, we have developed a drug-free, multipotent system for alleviating RA by suppressing inflammation and promoting tissue preservation.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5682–5696"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Functionalization of Thermoplastic Polyurethane Scaffolds to Enhance Microparticle Attachment.","authors":"Megan C Welch, Courtney K Rowe, Kelly A Burke","doi":"10.1021/acsbiomaterials.6c01093","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.6c01093","url":null,"abstract":"<p><p>While the use of polymer micro- and nanoparticles for drug delivery has been demonstrated for diverse biomedical applications, not all techniques have allowed for the precise localization of the drug that is needed to support surrounding tissues. Herein, we report a biocompatible thermoplastic polyurethane silk fibroin composite drug delivery platform for localized drug release. The work leverages two distinct materials, combining the biocompatibility of silk fibroin protein particles as a drug carrier with the modularity of a thermoplastic polyurethane scaffold, whose mechanical properties are tuned by composition. Two different particle attachment strategies were investigated; a Michael addition reaction was found to result in a high number of particles attached and a more complete surface coverage than carbodiimide coupling that is often employed with silk-based materials. The attachment of silk fibroin microparticles allows localized delivery of molecules, and the incorporation of poly(tetrahydrofuran) (pTHF) into polyurethane was found to tailor phase separation of the hard and soft segments that, in turn, affects the crystallinity and stiffness of the scaffold. The hard segment crystallinity of unprocessed powders (range: 16.26-35.51%) and slow-cooled films (range: 9.04-37.29%) increased as the pTHF content increased, indicating an increase in phase separation. The Young's modulus of thermoplastic polyurethanes (TPUs) also increased with increasing pTHF, from 9.02 to 14.49 MPa. In vitro cytocompatibility testing with human dermal fibroblasts showed >85% viability and no significant changes in cell growth with respect to composition. This platform demonstrates a promising strategy by combining two distinct materials to provide localized drug delivery with potential application for longer-term biocompatible medical implants.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149163593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper Peroxide Nanodots Encapsulated in a Metal–Organic Framework as H2O2 Self-Supplying Agents for Enhanced Antibacterial Activity and Wound Healing","authors":"Yulan Zhao,&nbsp;Quan Xu,&nbsp;Shijie Zheng,&nbsp;Yi Liu,&nbsp;Zhiyong Song,&nbsp;Heyou Han","doi":"10.1021/acsbiomaterials.6c00345","DOIUrl":"10.1021/acsbiomaterials.6c00345","url":null,"abstract":"<p>Drug-resistant bacterial infections rank among the most critical public health threats across the globe. The development of novel antibiotics falls considerably behind the rise of bacterial resistance. The advancement of nanotechnology offers novel opportunities to address the challenges posed by drug-resistant bacterial infections. In recent years, copper-based nanomaterials have gained widespread application in chemodynamic therapy (CDT) due to their favorable nanoenzymatic activities. However, their therapeutic efficacy is seriously constrained by the insufficient H₂O₂ supply and overexpressed glutathione (GSH) within the infected wound microenvironment. Therefore, a H₂O₂ self-supplying and GSH-depleting nanosystem (CuO₂@ZIF-8) was rationally constructed by simply encapsulating copper peroxide (CuO₂) within a zeolitic imidazolate framework-8 (ZIF-8). Under the acidic microenvironment of the infection sites, ZIF-8 underwent degradation to liberate Zn²⁺ ions, which exhibited potent antibacterial activity. Meanwhile, after being degraded, ZIF-8 released CuO₂, which in the slightly acidic infected microenvironment decomposed to generate H₂O₂ and Cu²⁺. The released Cu²⁺ then catalyzed the H₂O₂ into highly toxic hydroxyl radicals (·OH), thus achieving a great antibacterial effect between CDT and Zn²⁺. In addition, Cu²⁺ could be reduced to Cu⁺ by the overexpressed GSH, further enhancing the efficacy of CDT. In vitro antibacterial assay showed that due to the antibacterial effect of CDT and Zn²⁺, the antibacterial rate of CuO₂ @ ZIF-8 reached as high as 98.8%, which was significantly superior to that of ZIF-8 or CuO₂ used alone. In vivo assay showed that CuO₂ @ ZIF-8 had good antibacterial effects and excellent wound healing with great in vivo biosafety. In conclusion, the CuO₂ @ ZIF-8 nanoplatform developed in this work has considerable promise for future biomedical applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5515–5526"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148954549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Mineralized Capsules for Haematococcus pluvialis Clustering and Functional Regulation","authors":"Peihan Kang,&nbsp;Yunyang Zhang,&nbsp;Xiangdong Kong,&nbsp;Ruibo Zhao","doi":"10.1021/acsbiomaterials.6c00751","DOIUrl":"10.1021/acsbiomaterials.6c00751","url":null,"abstract":"<p>Astaxanthin is a high-value ketocarotenoid with potent antioxidant activity and wide applications in nutraceutical, cosmetic, and aquaculture industries. The green microalga <em>Haematococcus pluvialis</em> represents the most promising natural source; however, its efficient production requires precise control of growth and stress conditions, while free cells remain susceptible to environmental and mechanical stress. Hydrogel encapsulation offers a protective microenvironment, yet conventional alginate systems are limited by poor mechanical stability and uncontrolled swelling. Here, we report calcium alginate-based biomimetic mineralized core-shell capsules for the encapsulation, cultivation, and high-light-induced astaxanthin production of <em>H. pluvialis</em>. Capsules were fabricated by ionic crosslinking of alginate containing KH₂PO₄ with a CMC/CaCl₂ precursor solution, enabling in situ calcium phosphate formation within the capsule shell. Comprehensive characterization confirmed successful mineralization, reduced swelling, enhanced inorganic content, and improved mechanical strength. Encapsulated cells exhibited sustained growth and metabolic activity under moderate light without cytotoxic effects. Upon high-light exposure, both free and encapsulated cells showed a characteristic transition from green to red, accompanied by a significant increase in astaxanthin content. Notably, astaxanthin accumulation was comparable to that of controls. This study establishes a scalable encapsulation strategy that enhances structural stability without compromising biological function, offering potential for industrial microalgal cultivation and astaxanthin production.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"12 10","pages":"5380–5389"},"PeriodicalIF":6.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructural Anisotropy in Bone-Mimetic Silk Constructs Promotes Self-Mineralization.","authors":"Anyelo R Diaz, Yunteng Cao, Liliana Liverani, Aldo R Boccaccini, Benedetto Marelli, Chiara E Ghezzi","doi":"10.1021/acsbiomaterials.6c00931","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.6c00931","url":null,"abstract":"<p><p>Understanding how organic matrix architecture regulates biomineralization remains a central challenge in mineralized tissue regeneration. Emerging evidence highlights the importance of coupled phenomena, including nanoconfinement, ionic and pH regulation, and hierarchical structural remodeling, in directing mineral formation. Silk fibroin (SF) is a versatile structural protein widely used in biomaterials due to its tunable mechanics, scalable processing into micro- and nanoscale constructs, controllable biodegradation, and ability to support cell infiltration and tissue integration, including in bone. However, despite its favorable biological properties, SF exhibits limited intrinsic self-mineralization in physiological fluids, restricting its mechanical reinforcement and osteogenic potential. In this study, we engineered SF scaffolds with controlled microstructure and anisotropy and incorporated bioactive inorganic phases, nano-hydroxyapatite (HA) or 45S5 bioactive glass (BG) to promote self-directed mineralization. Scaffolds were evaluated in simulated body fluid under static and dynamic conditions to assess mineral formation and distribution. Isotropic architectures supported more uniform, volumetric mineral integration, whereas anisotropic scaffolds promoted preferential surface mineral deposition. Incorporation of HA or BG significantly enhanced mineralization, leading to the formation of carbonate-substituted hydroxyapatite (CHA). These compositional and structural effects translated into marked improvements in compressive mechanical properties. Collectively, these findings demonstrate that synergistic control over scaffold architecture and bioactive cargo can direct in situ mineralization, enabling the design of silk-based constructs with enhanced mechanical performance and potential for bone regeneration.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149163303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioenergetic and Meniscus-Inspired Anisotropic Hydrogel Scaffolds Loaded with Synovial Mesenchymal Stem Cells for Enhanced Meniscus Tear Repair.","authors":"Yixi Li, Lei Liu, Dan Zhou, Saijiao Lan, Lingtian Lu, Zijie Zhang, Lei Zhao, Jian Zhu, Xiang Zhang, Wenping Pan, Fang Wang, Hongmei Liu, Decheng Wu","doi":"10.1021/acsbiomaterials.6c00999","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.6c00999","url":null,"abstract":"<p><p>Meniscal tears have limited spontaneous healing due to poor vascularization and intrinsic repair capacity, often leading to post-traumatic osteoarthritis. Current clinical treatments fail to restore native meniscal structure and function, necessitating advanced regenerative strategies. Tissue-engineered scaffolds offer promise, yet conventional designs lack the anisotropic architecture and cannot mitigate the oxidative injury microenvironment. Herein, we developed a meniscus-inspired anisotropic hydrogel scaffold incorporating succinate for bioenergetic regulation and loaded with synovial mesenchymal stem cells (SMSCs) for repair of red-red and red-white transitional zones. The anisotropic structure was fabricated via freeze-casting to mimic native meniscal alignment, while succinate modification enabled bioenergetic regulation by reducing intracellular ROS levels, preserving mitochondrial homeostasis, and maintaining cellular energy status, thereby enhancing SMSC proliferation and migration. The scaffold improved SMSC behavior in vitro and promoted fibrocartilage-like meniscal repair while attenuating cartilage degeneration in a rabbit tear model. This bioenergetic, biomimetic scaffold integrates structural anisotropy, stem cell therapy, and metabolic regulation, representing a promising strategy for fibrocartilage-like meniscal repair and attenuation of secondary articular cartilage degeneration.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}