ACS Biomaterials Science & Engineering最新文献

{"title":"Characterization of Azido-Incorporated <i>Bombyx mori</i> Silk Fibroin as a Drug Carrier Material.","authors":"Yaxi Tian, Hidetoshi Teramoto","doi":"10.1021/acsbiomaterials.5c00232","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00232","url":null,"abstract":"<p><p>Silk fibroin, a natural polymer derived from the domesticated silkworm, <i>Bombyx mori</i>, exhibits remarkable tensile toughness, broad biocompatibility, and biodegradability. We previously developed azido-incorporated silk fibroin (<i>AzidoSilk</i>) using genetic code expansion. <i>AzidoSilk</i> contains synthetic azido groups that can be selectively attached to any functional molecule in a bioorthogonal manner through click chemistry. Click chemistry provides high yields and minimal byproducts. In this study, <i>AzidoSilk</i> was characterized as a drug carrier material for on-demand drug delivery systems (DDS) because effective drug loading and controllable release by external stimuli can be achieved with <i>AzidoSilk</i> via click chemistry modifications. Fluorescent drug models were immobilized on <i>AzidoSilk</i> film and woven fabric via a UV-sensitive bifunctional linker using click chemistry. Azido-selective immobilization of the drug models was confirmed, and upon irradiation with 365 nm UV light, the drug models were gradually released from the <i>AzidoSilk</i> materials in a time-dependent manner. In another model, kanamycin was immobilized on <i>AzidoSilk</i> fabric via the same UV-sensitive linker, and its antibacterial activity against <i>Staphylococcus aureus</i> was tested. PBS extracts from kanamycin-immobilized <i>AzidoSilk</i> fabrics after UV irradiation showed significant antibacterial activity against <i>S. aureus</i>. These results demonstrate that <i>AzidoSilk</i> can be used as a drug carrier material for on-demand DDS. In this system, changes in linker design can expand the range of external stimuli usable for drug release, depending on the application. <i>AzidoSilk</i> has broadened the scope of chemical modification of silk fibroin to achieve simpler and more reliable drug delivery.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2783-2791"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Strategies in Enhancing the Hepatoprotective Efficacy of Natural Products: Integrating Nanotechnology, Genomics, and Mechanistic Insights.","authors":"Jitendra Patel, Harekrishna Roy, Pavan Kuma Chintamaneni, Rukmani Patel, Raghvendra Bohara","doi":"10.1021/acsbiomaterials.5c00004","DOIUrl":"10.1021/acsbiomaterials.5c00004","url":null,"abstract":"<p><p>Liver disorders like hepatitis, cirrhosis, and hepatocellular carcinoma present a significant global health challenge, with high morbidity and mortality rates. Key factors contributing to liver disorders include inflammation, oxidative stress, and apoptosis. Due to their multifaceted action, natural compounds are promising candidates for mitigating liver-related disorders. Research studies revealed the antioxidant, anti-inflammatory, and detoxifying properties of natural compounds like curcumin, glycyrrhizin, and silymarin and their potential for liver detoxification and protection. With advancements in nanotechnology in drug delivery, natural compounds have improved stability and targetability, thereby enhancing their bioavailability and therapeutic efficiency. Further, recent advancements in genomics and an increased understanding of genetic factors influencing liver disorders and the hepatoprotective effects of natural agents made way for personalized medicine. Moreover, combinatorial therapy with natural products, synthetic drugs, or other natural agents has improved therapeutic outcomes. Even though clinical trials have confirmed the efficiency of natural compounds as hepatoprotective agents, several challenges remain unanswered in their translation to clinical practice. Therefore, it is logical to integrate natural compounds with nanotechnology and genomics to further advance hepatoprotection. This review gives an overview of the substantial progress made in the field of hepatoprotection, with specific emphasis on natural compounds and their integration with nanotechnology and genomics. This provides valuable insights for future research and innovations in developing therapeutic strategies for liver disorders.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2528-2549"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse Bone Matrix and Mineral Alterations in Osteoporosis with Different Causes: A Solid-State NMR Study.","authors":"Jing-Yu Lin, Xu Guo, Ming-Hui Sun, Yifeng Zhang, Jun-Xia Lu","doi":"10.1021/acsbiomaterials.4c01581","DOIUrl":"10.1021/acsbiomaterials.4c01581","url":null,"abstract":"<p><p>Osteoporosis (OP), which is a common skeletal disease with different causes, is prevalent in the aging population. Postmenopause women generally suffer from OP with bone loss due to estrogen deficiency. Diabetes is also associated with OP by complex metabolic mechanisms. Bone qualities of OP caused by aging were compared with those of the ovariectomy (OVX) model and the Type 2 diabetic model using Sprague-Dawley (SD) rats in our study. Combining with micro-computed tomography (μ-CT) and solid-state NMR (SSNMR) methods, this research studied bone changes in SD rats from tissue level to the molecular level. The studies revealed bone loss was most significant for cancellous bones but not for cortical bones in OP rats. However, at the molecular level, the content of HAP in cortical bone increased with aging, contributing to the brittleness of the bone. Triglyceride, as a senescence maker of osteocyte in cortical bone, was also identified to be closely associated with OP in aging and OVX rats but not in diabetic rats. This research suggests that changes of bone quality at the molecular level more objectively reflect the bone tissue reconstruction of OP with various causes rather than mere bone loss revealed by μ-CT analysis.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2974-2987"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Therapeutic Effects of Prussian Blue Erbium-Doped Hydroxyapatite Nanoparticles in Photothermal Photodynamic Cancer Therapy.","authors":"Thi Thuy Truong, Vu Hoang Minh Doan, Dinh Quan Nguyen, Quoc Dung Nguyen, Jaeyeop Choi, Bharathiraja Subramaniyan, Jaesung Ahn, Byeongil Lee, Junghwan Oh, Sudip Mondal","doi":"10.1021/acsbiomaterials.5c00027","DOIUrl":"10.1021/acsbiomaterials.5c00027","url":null,"abstract":"<p><p>This study explores the synergistic therapeutic potential of Prussian Blue Erbium-Doped Hydroxyapatite (PB-Er-HAp) bioceramics in the context of photothermal therapy (PTT) and photodynamic therapy (PDT) for cancer treatment, highlighting their role in multimodal therapeutic approaches and imaging. PB-Er-HAp nanoparticles (NPs) were synthesized using a facile coprecipitation method to incorporate erbium (Er) into nanostructured hydroxyapatite (HAp) at various concentrations. Prussian Blue (PB) was functionalized onto the surfaces of these NPs, resulting in a final particle size of less than 50 nm. The therapeutic efficacy of the synthesized 1.0 mol % PB-Er-HAp NPs was evaluated <i>in vitro</i>, using MDA-MB-231 breast cancer cells. <i>In vitro</i> studies demonstrated that the PB-Er-HAp NPs exhibited significant PTT and PDT effects under 808 nm laser irradiation, effectively inducing cancer cell death through heat generation and reactive oxygen species production, respectively. <i>In vitro</i> experiments validated the ability of NPs to inhibit tumor growth in the MDA-MB-231 breast cancer cell line. This study emphasizes the potential of PB-Er-HAp NPs as a versatile platform for synergistic cancer therapy, combining PTT and PDT effects, while offering capabilities for biomedical imaging. Future research aims to further optimize these NPs and explore their clinical application, aiming toward enhanced therapeutic outcomes in cancer treatment.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2639-2652"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multichannel Continuum Robot for <i>In Situ</i> Diagnosis and Treatment of Vascular Lesions.","authors":"Wei Liu, Qinzhou Luo, Xintao Zhu, Ming Liu, Ligang Yao, Fanan Wei","doi":"10.1021/acsbiomaterials.5c00113","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00113","url":null,"abstract":"<p><p>In recent years, continuum soft robots have emerged as a promising avenue for the advancement of <i>in vivo</i> therapeutic interventions. However, the current continuum robots are often limited to singular functionalities and exhibit a deficiency in diagnostic capabilities for vascular lesions. For example, vasculitis often leads to temperature abnormalities in local blood vessels, and the existing continuum robots are unable to accurately detect the lesion area based on this characteristic. To address this issue, this paper presents the design of a multifunctional integrated thermally drawn polymer multichannel continuum robot. First, the magnetic deformation of the continuum robot was theoretically analyzed, and the robot's locomotion within a flow field was experimentally verified. Moreover, different channels of the multichannel continuum robot were independently designed for specific functions, enabling multithreaded operations. It can perform real-time sensing and monitoring of external environmental temperatures with high resolution and carry out targeted drug delivery as well as neural electrical stimulation. We successfully conducted <i>in vitro</i> experiments on isolated frog sciatic nerves, confirming the effectiveness of the multichannel continuum robot for biological treatment. The multichannel continuum robot shows great potential in the diagnosis and treatment of vasculitis <i>in situ</i> and nerve system disorder.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3071-3081"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Locally Delivered Hydrogel with Sustained Release of Flavonol Compound Kaempferol Mitigates Inflammatory Progression of Periodontitis and Enhances the Gut Microflora Composition in Rats\".","authors":"Ningli Li, Mingzhen Yang, Miaomiao Feng, Xiaoran Xu, Yingying Li, Yonghong Zhang, Cory J Xian, Tiejun Li, Yuankun Zhai","doi":"10.1021/acsbiomaterials.5c00594","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00594","url":null,"abstract":"","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3082"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycated Cross-Linked Collagen Membranes with Tunable Permeability and Multifunctional Properties for Tissue Regeneration.","authors":"Mina Vaez, Marianne Odlyha, Sumaiya Farzana, Patrick C Lee, Boris Hinz, Laurent Bozec","doi":"10.1021/acsbiomaterials.5c00120","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00120","url":null,"abstract":"<p><p>Interface tissue engineering focuses on developing bioengineered constructs that integrate with the body's natural tissues. Collagen-based membranes, due to their inherent bioactivity and compatibility, are widely used in tissue engineering applications such as wound healing, guided tissue regeneration, and guided bone regeneration. This study investigates the <i>in vitro</i> development and characterization of methylglyoxal (MGO)-cross-linked collagen membranes, which exhibit enhanced mechanical strength, thermal stability, hydrophilicity, and tunable permeability. To evaluate the properties of these membranes, we employed several techniques, including scanning electron microscopy for morphological analysis, differential scanning calorimetry for thermal stability assessment, tensile strength tests for mechanical evaluation, water contact angle measurements for wettability, dielectric analysis for moisture absorption, and permeability assays using fluorescein diffusion. Additionally, the fibroblast barrier function was assessed using a red cell tracking dye with confocal microscopy. The ability to fine-tune the properties of collagen membranes through MGO cross-linking opens new possibilities for their use in tissue engineering. These membranes can serve as effective barriers in guided tissue regeneration and guided bone regeneration, promoting tissue regeneration and healing by preventing undesired cell migration and creating a conducive environment for bone and tissue growth. MGO-cross-linked collagen membranes offer a promising solution for enhancing the functionality and efficacy of bioengineered constructs in tissue engineering. Their improved mechanical and thermal properties, coupled with their biocompatibility, make them ideal candidates for various clinical applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2946-2957"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antithrombotic Effect of a Bivalent DNA Aptamer of Thrombin.","authors":"Yanxi Chen, Shoubo Xiang, Chunfa Chen, Qiuyu Su, Zhe Zhang, Yangyang Fan, Zhihong Cui, Lixue Yin, Hua Zuo, Mingliang Zuo","doi":"10.1021/acsbiomaterials.5c00222","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00222","url":null,"abstract":"<p><p>Thrombin plays a critical role in both coagulation and platelet activation, and its interaction with thrombin-protease-activated receptor 1 (PAR1) on platelets and vascular smooth muscle cells (VSMCs) leads to a series of pathological processes such as thrombosis, restenosis, and atherosclerosis. This study investigated the antithrombotic properties of a bivalent DNA aptamer (bApt) with phosphorothioate backbone modification designed to inhibit thrombin, with a specific focus on its ability to regulate the thrombin-PAR1 signaling pathway. The results showed that bApt modulated the thrombin-PAR1 pathway, effectively reduced thrombus formation, platelet aggregation, and VSMC proliferation. Key findings from the study highlight that bApt successfully prolonged coagulation reaction time (<i>R</i> value), coagulation time (<i>K</i> value), maximum amplitude (MA) and reduced coagulation angle (α value), and also prolonged thrombin time (TT) and activated partial thromboplastin time (APTT), in a dose-dependent manner. Moreover, in an arterial injury model, bApt reduced thrombus formation significantly, supporting its potential as a therapeutic agent for thrombotic diseases.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2705-2712"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Breast Cancer Patient-Specific Metastasis Severity at Bone Site Using <i>In Vitro</i> Models.","authors":"Preetham Ravi, Shrinwanti Ghosh, Pooyan Vahidi Pashaki, Kalidas Shetty, Jiha Kim, Anu Gaba, Dinesh R Katti, Kalpana S Katti","doi":"10.1021/acsbiomaterials.4c01599","DOIUrl":"10.1021/acsbiomaterials.4c01599","url":null,"abstract":"<p><p>As breast cancer progresses to stage IV, it metastasizes to secondary organs, with a strong propensity for bone colonization. Bone metastasis results in dramatically decreased survival rates and currently lacks a definitive cure. To improve survival rates significantly, there is a need for complex and precise <i>in vitro</i> models that can accurately replicate advanced-stage breast cancer for drug screening purposes. Previously, we established a 3D nanoclay <i>in vitro</i> model of bone metastatic breast cancer using human mesenchymal stem cells in combination with either commercial breast cancer cells (MCF-7 and MDA-MB-231) or patient-derived cells (NT013 and NT023) from the primary breast cancer site. In the present study, the efficacy of the <i>in vitro</i> model to distinguish and differentiate between the severity of metastasis in a total of eight patient-derived cell lines representing various subtypes was evaluated. We also tested the effects of the phytochemically enriched plant extract, <i>Rhodiola crenulata</i><i>, on eight patient-derived cell lines (NT015, NT017, NT021, NT042, NT045, and NT046, in addition to NT013 and NT023)</i> in bone metastatic (BM) culture. Our results confirmed that the cell lines maintained their subtype-specific characteristics after isolation and formed tumors within the bone microenvironment. Additionally, we assessed the impact of these cell lines on Wnt signaling pathways, identifying which lines upregulate or downregulate Wnt signaling through ET-1 and DKK-1 cytokine levels. Within each subtype, we observed differences in the severity of metastasis between patients. <i>R. crenulata</i> induced cytotoxicity in most patient-derived BM cultures, though NT042 BM cultures showed minimal response. In summary, our study has established a patient-derived bone-metastatic breast cancer model that is well-suited for personalized drug screening aimed at treating late-stage breast cancer. This bone metastatic testbed has the capability to evaluate the severity of metastasis within breast cancer subtypes for individual patients.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2824-2833"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Innovations of Antimicrobial Polymers for Combating Antimicrobial Resistance.","authors":"Zhangyong Si, Mary B Chan-Park","doi":"10.1021/acsbiomaterials.4c02147","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02147","url":null,"abstract":"<p><p>The global rise of antimicrobial resistance (AMR) has rendered many traditional antibiotics ineffective, leading to an urgent need for alternative therapeutic strategies. Antimicrobial polymers, with their ability to rapidly kill bacteria by disrupting or crossing membranes and/or targeting multiple microbial functions without inducing resistance, offer a promising solution. This perspective explores recent innovations in the design and synthesis of antimicrobial polymers, focusing on their chemical motifs, structural derivatives, and their applications in combating systemic and topical infections. We also highlight key challenges in translating these materials from laboratory research to clinical practice, including issues related to the high dose required, bioavailability and stability in systemic infection treatment, and ability to disperse and kill biofilms in localized infection management. By addressing these challenges, antimicrobial polymers could play a crucial role in the development of next-generation therapeutics to combat multidrug-resistant pathogens. This perspective attempts to summarize significant insights for the design and development of advanced antimicrobial polymers to overcome AMR, offering potential pathways to improve clinical outcomes in treating systemic and local infections.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2470-2480"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}