ACS Biomaterials Science & Engineering最新文献

{"title":"Self-Assembly Oligomeric Anthocyanin-Based Core-Shell Structure of Nanoparticles Enhances the Delivery and Efficacy of Berberine in Osteoarthritis.","authors":"Yao Huang, Xie Wang, Huikun Chen, Yu Wu, Lei Lv, Feilong Chen, Hanqi Lei, Chengyuan Xing","doi":"10.1021/acsbiomaterials.5c00037","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00037","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a degenerative joint disease that significantly contributes to functional disability, primarily due to inflammation and cell apoptosis. Berberine (BBR) has demonstrated notable anti-inflammatory and antiapoptotic effects in the treatment of OA. However, despite its promising pharmacological properties, the naturally occurring hydrophobic properties and limited solubility in water restrict the efficacy of BBR. Therefore, excipients are required to modify BBR. Oligomeric proanthocyanidins (OPAs) are dimers, trimers, and tetramers of proanthocyanidins (PAs). The unique interface properties of the OPAs underscore their potential as drug carriers. OPAs as natural carriers enhance medication effectiveness and significantly reduce the incidence of side effects. Herein, we developed natural self-assembled nanoparticles between BBR and the OPAs (BBR-OPAs NPs). By adopting the unification of medicines and excipients, the OPAs-based drug delivery system serves as an effective carrier and exerts therapeutic effects in OA treatment. The formation of BBR-OPAs NPs has been core-shell structure, as confirmed by transmission electron microscopy (TEM), 2D NOESY spectroscopy, and molecular dynamics (MD) simulation. The BBR-OPAs NPs exhibited good long-acting release capability due to their strong noncovalent interactions, making them competitive candidates for treating OA. Microcomputed tomography (micro-CT) scanning and histological evaluation further confirmed the efficacy of BBR-OPAs NPs in treating OA. In vivo assessments demonstrated that BBR-OPAs NPs inhibited inflammation and apoptosis, thereby preventing the progression of OA. Furthermore, treatment with BBR-OPAs NPs can inhibit synovial inflammation and protect chondrocytes. OPAs show broad prospects as drug delivery carriers and exhibit great potential in the treatment of OA.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2739-2752"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric Heart Valve Leaflets Tested in an Accelerated Wear Tester Revealed a Stable Craze Microstructure.","authors":"Nipa Khair, Katie Vinterella, David Ethan Harrell, Julianne Kindsfater, Lakshmi Prasad Dasi, Susan P James","doi":"10.1021/acsbiomaterials.4c02412","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02412","url":null,"abstract":"<p><p>Rheumatic and calcified aortic heart valve disease is a global health concern, impacting millions of individuals across various age groups. The gold standard medical treatments recommend replacing the sick heart valves with bioprosthetic valves that are chemically fixed using glutaraldehyde, commonly sourced from bovines or porcines. Clinical investigations over more than two decades have revealed that fixed tissues are prone to premature calcification and tearing, thereby limiting their durability. An innovative alternative approach involves biomolecule-enhanced polyethylene-based linear low-density polyethylene. LLDPE thin films have high tear strength and excellent flexibility, making them an appealing choice for developing heart valves. Nonetheless, during durability testing according to the ISO 5840-2005 standards, these leaflets exhibited premature failure. The leaflets consistently wear and tear around highly stressed commissure posts. Nine of these worn leaflets were retrieved from the failed valves and chemically etched. The semicrystalline LLDPE polymer underwent chemical etching using a standard 2% w/v permanganate etching solution, followed by multistep washing. SEM analysis of virgin LLDPE unveiled distinctive spherulitic structures consisting of well-organized lamellae with diameters of approximately 3 μm and dimensions below 100 nm. The etching process effectively eliminated low-energy amorphous regions, revealing the spherulites. A similar study was carried out on the damaged leaflets. The SEM images displayed signs of surface wear and aligned areas of polymeric material oriented perpendicular to the principal stress direction. Following etching, some of the built-up remained partially intact, while other areas exposed the crystals beneath them. Remarkably, one of the worn samples unveiled the Kramer craze microstructure \"cross-tie\" composed of aligned fibrils and interlinked fibrils. The spacing between cross-tie lamella ranges between 100 and 200 nm and the thickness remains at 40-80 nm. To the best of the author's knowledge, a cross-tie structure has only been theorized with indirect evidence collected from laboratory-grown crazes. These findings are further confirmed with SAXS.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3058-3070"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.","authors":"Leonardo Boccoli, Elena Drago, Andrea Cafarelli, Lorenzo Vannozzi, Angelo Sciullo, Federica Iberite, Sajedeh Kerdegari, Toshinori Fujie, Emanuele Gruppioni, Claudio Canale, Leonardo Ricotti","doi":"10.1021/acsbiomaterials.4c02468","DOIUrl":"10.1021/acsbiomaterials.4c02468","url":null,"abstract":"<p><p>Biohybrid actuators exploit the contraction of biological components (muscle cells) to produce a force. In particular, bottom-up approaches use tissue engineering techniques, by coupling cells with a proper scaffold to obtain constructs undergoing contraction and guaranteeing actuation in biohybrid devices. However, the fabrication of actuators able to recapitulate the organization and maturity of native muscle is not trivial. In this field, quasi-two-dimensional (2D) substrates are raising interest due to their high surface/thickness ratio and the possibility of functionalizing their surface. In this work, we fabricated micropatterned thin films made of poly(styrene-butadiene-styrene) (SBS) doped with barium titanate nanoparticles (BTNPs) for fostering myogenic differentiation. We investigated material concentrations and fabrication process parameters to obtain thin microgrooved films with an average thickness below 1 μm, thus featured by a relatively low flexural rigidity and with an anisotropic topography to guide cell alignment and myotube formation. The embodiment of BTNPs did not significantly affect the film's mechanical properties. Interestingly, the presence of BTNPs enhanced the expression of myogenic differentiation markers (i.e., MYH1, MYH4, MYH8, and ACTA1). The results show the promising potential of SBS thin films doped with BTNPs, opening avenues in the fields of biohybrid actuation and skeletal muscle tissue engineering.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2910-2921"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erythrocyte Membrane-Camouflaged Xanthohumol Nanoparticles Mitigate Doxorubicin-Induced Cardiotoxicity by Inhibiting Ferroptosis.","authors":"Jingchao Li, Yinghua Zeng, Fengjiao Liu, Xu Liao, Chongbin Zhong, Shujuan Dong, Yanbin Cai, Pingzhen Yang","doi":"10.1021/acsbiomaterials.4c02467","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02467","url":null,"abstract":"<p><p>Doxorubicin (DOX) chemotherapy is a cornerstone of cancer treatment, but its clinical application and effectiveness are severely restricted due to its life-threatening cardiotoxicity. Xanthohumol (XH), a compound from traditional Chinese medicine, is noted for its antioxidant properties and the potential to mitigate DOX-induced cardiotoxicity (DIC). However, its poor water solubility results in low biocompatibility, making it susceptible to immune system clearance, which severely restricts its application in vivo. In this study, we first identified and demonstrated that XH can effectively mitigate DIC by inhibiting ferroptosis. We designed a biomimetic nanodelivery system encapsulating XH within porous poly(lactic-<i>co</i>-glycolic acid) (PLGA) nanoparticles, further coated with an erythrocyte membrane (XH-NPs@RBCm). This system offers several advantages, including evasion of macrophage phagocytosis and prolonged circulation time, thereby enhancing the stability and bioavailability of XH in vivo. Treatment with XH-NPs@RBCm significantly reduced reactive oxygen species-dependent ferroptosis, improving the DOX-induced myocardial atrophy and cardiac dysfunction. Our study underscores the therapeutic promise of XH-NPs@RBCm in treating DIC through ferroptosis inhibition, offering key insights into biomimetic nanodelivery system development for DIC management.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2727-2738"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles.","authors":"Andrew R Hudson, Daniel J Shiwarski, Alec J Kramer, Adam W Feinberg","doi":"10.1021/acsbiomaterials.4c02169","DOIUrl":"10.1021/acsbiomaterials.4c02169","url":null,"abstract":"<p><p>Current limitations in engineered tissues arise from the inability to provide sufficient nutrients to cells deep within constructs, restricting their viability. This study focuses on enhancing diffusion by creating a microporous microenvironment using gelatin microparticles within collagen scaffolds. By leveraging the FRESH (Freeform Reversible Embedding of Suspended Hydrogels) 3D bioprinting technique, gelatin microparticles are utilized both as a support material and as a thermoresponsive porogen to establish interconnected pores. The results indicate that scaffolds with 75% porosity significantly increase diffusion rates and cell viability, extending beyond the conventional ∼200 μm limit. Additionally, integrating vascular-like channels with porous scaffolds and applying perfusion improved nutrient transport, leading to enhanced cell survival in larger constructs. This combination of microporosity and perfusion represents a promising approach to create thicker tissues without necrotic regions, potentially paving the way for scalable tissue engineering applications. The findings suggest that optimizing pore sizes and scaffold perfusion can bridge the gap between rapid tissue formation and slower vascularization processes, enabling the future development of functional tissue constructs at clinically relevant scales.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2888-2897"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Si<i>x</i>-Axis, Physiological Activity Profiles Create a More Challenging Cellular Environment in the Intervertebral Disc Compared to Single-Axis Loading.","authors":"Daniela Lazaro-Pacheco, Isabelle Ebisch, Justin Cooper-White, Timothy P Holsgrove","doi":"10.1021/acsbiomaterials.4c01773","DOIUrl":"10.1021/acsbiomaterials.4c01773","url":null,"abstract":"<p><p>Bioreactors provide a valuable way to explore interactions between the mechanical and biological environments of the intervertebral disc (IVD), but the replication of ecologically valid loading protocols is a huge challenge. The aim of this study was to address this through the combination of time use survey data and six-axis load data from <i>in vivo</i> measurements during functional movements and activities of daily living to create population-based activity profiles, which were employed using a unique si<i>x</i>-axis bioreactor and a whole-organ bovine tail IVD model. The results of the study show that six-axis activity profiles create a more challenging environment compared to single-axis loading or unloaded controls, resulting in lower cell viability in both the nucleus pulposus and annulus fibrosus regions of the IVD. Additionally, the six-axis activity profile representing a more active lifestyle led to an even lower cell viability in the annulus fibrosus, which may be due to the increased strains in this region of the IVD during activities of daily living. These findings highlight the importance of considering a wide range of activities and lifestyles in the development and evaluation of regenerative therapies and preventative interventions for IVD, if they are to be successfully translated to the clinical setting.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3031-3042"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Powered Oxygen Microbubble Generator for Decontamination of Anaerobic Biofilm-Fouled Bioimplants.","authors":"Eun-Hyuk Lee, Hyunsub Kim, Joo Hun Lee, Youngjoon Kim, Ho-Beom Kwon, Young-Jun Lim, Hyunjoon Kong, Sang-Woo Lee, Myung-Joo Kim","doi":"10.1021/acsbiomaterials.5c00303","DOIUrl":"10.1021/acsbiomaterials.5c00303","url":null,"abstract":"<p><p>Biomedical devices often feature a microgap: confined, minuscule spaces that foster bacterial infiltration and biofilm formation. For instance, peri-implantitis with prevalence rates of 4.7-45% at the patient level is a major complication driven by biofilm infections, characterized by chronic inflammation and implant failure. Anaerobic biofilm residing within the microgap serves as a major source of the peri-implantitis, but tools that remove the biofilm are lacking. Therefore, this study presents a novel preventive strategy employing <b>s</b>elf-powered <b>m</b>icrobubbler (SM) for targeted decontamination of micrographs in dental implants. SMs are assembled by doping diatoms with MnO₂ nanosheets. These particles are activated to generate O₂ microbubbles in H₂O₂ solution via catalase-mimetic activity and can penetrate the biofilm structures. The resulting oxygen bubbles induce effective mechanical disruption and oxygenation within biofilm-mimicking gelatin hydrogels and <i>Porphyromonas gingivalis</i> biofilms found in the peri-implantitis-affected implants. Such biofilm removal from the microgap restored mechanical stability at implant abutment-fixture connections and reduced bacterial leakage. Multispecies biofilms from patient-derived implants were similarly decontaminated with the mixture of SM-H₂O₂ outperforming conventional antiseptics like 0.2% chlorhexidine and 3% H₂O₂ alone. This innovative approach extends beyond dental implants to address biofilm-associated challenges in various biomedical devices with microgap vulnerabilities. Overall, SM-based treatments will offer an efficient and nondamaging solution to enhance the sterility and longevity of various bioimplants with intricated and confined structure.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3019-3030"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Osteogenic Activity of a Titanium Mesh Modified with Magnesium-Doped Nanowires for Peri-Implant Guided Bone Regeneration: In Vitro and In Vivo.","authors":"Zhe Shen, Ya-Wen Zhu, Yu-Wen Wei, You Zhou, Yan Xu, Wei Chen, Jing Qiu","doi":"10.1021/acsbiomaterials.4c01854","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01854","url":null,"abstract":"<p><p>Titanium mesh is a promising barrier membrane for the reconstruction of alveolar bone defects, with the quality and volume of alveolar bone being critical factors impacting the initial stability and success rate of implants. The objective of this study is to integrate bioactive magnesium ions and nanowire structures into a titanium mesh surface (Mg-NW-Ti) and further investigate its surface characteristics and osteogenic bioactivity in vitro and in vivo. Mg-NW-Ti was effectively synthesized through a series of chemical reactivity tests, and its morphology, roughness, hydrophilicity, elemental composition, and ion release were characterized. The biological effects of Mg-NW-Ti on MC3T3-E1 cells were assessed and compared with commercially pure titanium (CP-Ti) and nanowire-modified titanium (NW-Ti). In addition, a peri-implant bone defect model of rabbit mandibular alveolar bone was constructed to evaluate the effects of Mg-NW-Ti mesh on bone regeneration and osseointegration of the implant. The resultant Ti surface appeared as a nanowire structure under scanning electron microscopy with higher surface roughness and hydrophilicity compared to the CP-Ti. The X-ray photoelectron spectroscopy and ion release analysis demonstrated successful loading of magnesium ions onto the titanium surface and effective release into the surroundings. In vitro Mg-NW-Ti exhibited good biocompatibility and significantly enhanced proliferation and differentiation of MC3T3-E1, while the results of the in vivo study demonstrated that the Mg-NW-Ti mesh exhibited a beneficial impact on bone regeneration and implant osseointegration. In conclusion, this novel surface modification of titanium mesh may serve as an effective strategy for optimizing the osteogenic functionality of titanium mesh and harnessing its potential for increased application value.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2664-2676"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication of HepG2 Cells-Laden 3D Structures Using Nanocellulose-Reinforced Gelatin-Based Hydrogel Bioinks: Materials Characterization, Cell Viability Assessment, and Metabolomic Analysis.","authors":"Nicole S Lameirinhas, Maria C Teixeira, João P F Carvalho, Bruno F A Valente, Jorge L Luís, Iola F Duarte, Ricardo J B Pinto, Helena Oliveira, José M Oliveira, Armando J D Silvestre, Carla Vilela, Carmen S R Freire","doi":"10.1021/acsbiomaterials.4c02148","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02148","url":null,"abstract":"<p><p>The successful replication of the intricate architecture of human tissues remains a major challenge in the biomedical area. Three-dimensional (3D) bioprinting has emerged as a promising approach for the biofabrication of living tissue analogues, taking advantage of the use of adequate bioinks and printing methodologies. Here, a hydrogel bioink based on gelatin (Gel) and nanofibrillated cellulose (NFC), cross-linked with genipin, was developed for the 3D extrusion-based bioprinting of hepatocarcinoma cells (HepG2). This formulation combines the biological characteristics of Gel with the exceptional mechanical and rheological attributes of NFC. Gel/NFC ink formulations with different Gel/NFC mass compositions, viz., 90:10, 80:20, 70:30, and 60:40, were prepared and characterized. The corresponding cross-linked hydrogels were obtained using 1.5% (w/w) genipin as the cross-linking agent. The rheological and mechanical performances of the inks were enhanced by the addition of NFC, as evidenced by the rise in the yield stress from 70.9 ± 28.6 to 627.9 ± 74.8 Pa, compressive stress at 80% strain from 0.5 ± 0.1 to 1.5 ± 0.2 MPa, and Young's modulus from 4.7 ± 0.9 to 12.1 ± 1.1 MPa, for 90:10 and 60:40 inks, respectively. Moreover, higher NFC contents translated into 3D structures with better shape fidelity and the possibility of printing more intricate structures. These hydrogels were noncytotoxic toward HepG2 cells for up to 48 h, with cell viabilities consistently above 80%. The ink 70:30 was loaded with HepG2 cells (2 × 10⁶ cells mL^-1) and bioprinted. Cell viability remained elevated (90 ± 4%) until day 14 postbioprinting, with cells maintaining their metabolic activity shown by ¹H NMR metabolomics, proving the enormous potential of Gel/NFC-based bioinks for bioprinting HepG2 cells without jeopardizing their viability and metabolism.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"3043-3057"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Poly(ether sulfone)/Poly(vinyl alcohol)/Magnesium-Doped Carbon Quantum Dot Scaffolds for Bone Tissue Engineering.","authors":"Mehrab Pourmadadi, Hamidreza Abdouss, Salar Mohammadi Shabestari, Seyede Mahtab Hosseini, Narges Ajalli, Majid Abdouss, Rasoul Esmaeely Neisiany","doi":"10.1021/acsbiomaterials.4c02124","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02124","url":null,"abstract":"<p><p>Bone tissue engineering plays a critical role in overcoming the limitations of traditional bone grafts and implants by enhancing bone integration and regeneration. In this study, we developed a novel membrane scaffold comprising poly(ether sulfone) (PES), poly(vinyl alcohol) (PVA), and magnesium-doped carbon quantum dots (CQDs.Mg) for potential bone tissue engineering applications. Four distinct scaffold formulations (PE-CM0, PE-CM2, PE-CM3, and PE-CM4) were developed using a film applicator machine. The morphology and porosity of the scaffolds, characterized via scanning electron microscopy (SEM), revealed increased porosity with higher CQDs.Mg content. Fourier transform infrared spectroscopy (FTIR) confirmed the successful integration of functional groups from each component. Water contact angle (WCA) measurements indicated improved hydrophilicity with the addition of CQDs.Mg, which is beneficial for cell attachment and proliferation. Mechanical testing demonstrated that the scaffolds maintained adequate tensile strength and flexibility, with PE-CM3 and PE-CM4 exhibiting superior properties. Swelling assays indicated enhanced water absorption with increased CQDs.Mg content, while 14-day degradation studies showed excellent structural stability. Biocompatibility was also assessed using L929 and NIH3T3 cell lines, with cytotoxicity assays demonstrating nearly 100% cell viability across all samples. These findings suggest that the PES/PVA/CQDs.Mg scaffolds exhibit a promising combination of mechanical robustness, hydrophilicity, and biocompatibility, making them strong candidates for bone tissue engineering applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2846-2856"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}