ACS Biomaterials Science & Engineering最新文献_第8页

Optimizing Isolation Methods and Exploring the Therapeutic Potential of Lotus-Derived Extracellular Vesicles in Modulating Inflammation and Promoting Wound Healing. 优化分离方法，探索荷花源性细胞外囊泡调节炎症和促进伤口愈合的治疗潜力。

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-06-09 DOI: 10.1021/acsbiomaterials.5c00377

Kai-Jiun Lo, Mu-Hui Wang, Ching-Yao Kuo, Min-Hsiung Pan

{"title":"Optimizing Isolation Methods and Exploring the Therapeutic Potential of Lotus-Derived Extracellular Vesicles in Modulating Inflammation and Promoting Wound Healing.","authors":"Kai-Jiun Lo, Mu-Hui Wang, Ching-Yao Kuo, Min-Hsiung Pan","doi":"10.1021/acsbiomaterials.5c00377","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00377","url":null,"abstract":"In the past decade, with the rise of research on plant-derived extracellular vesicles (PDEVs), scientists have been continuously exploring the bioactivity of PDEVs. Many PDEVs have been shown to possess a variety of biological activities. Given that the specific characteristics of EVs are believed to be related to their source cells, PDEVs from traditional Chinese medicinal herbs hold significant potential for development. In this study, lotus (Nelumbo nucifera Gaertn.) leaves were selected as the source of PDEVs, and the impact of different isolation methods on their characteristics was evaluated, while their potential biological activities were also assessed. Lotus-derived EVs (LDEVs) were isolated by using tangential flow filtration (TFF), ultracentrifugation (UC), density gradient ultracentrifugation (DGU), and size-exclusion chromatography (SEC), respectively. The mean sizes of LDEVs isolated by various methods were in the range of 130-160 nm. Although the LDEVs isolated by the TFF method had a lower zeta potential, it exhibited the highest purity, with a yield of 3.69 ± 0.43 × 109 particles/g lotus leaves. Notably, LDEVs isolated by different methods all demonstrated the ability to attenuate LPS-induced inflammation in RAW264.7 cells, significantly decreasing the nitrite concentration in the culture medium. Furthermore, LDEVs also showed potential for wound healing, promoting the migration of HaCaT cells in vitro. LDEVs also demonstrated internalization by RAW264.7 and HaCaT cells. These results support the potential of LDEVs for biomedical applications while also suggesting that TFF is a promising and viable strategy for large-scale PDEV isolation.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SiRNA-Targeting TGF-β1 Based on Nanoparticle-Coated Ureteral Stents to Inhibit Ureteral Stricture. 基于sirna靶向TGF-β1的纳米输尿管支架抑制输尿管狭窄

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-06-09 Epub Date: 2025-05-13 DOI: 10.1021/acsbiomaterials.4c01925

Wei Meng, Ningning Li, Feng Lv, Bo Chen, Shuaijiang Lu, Jiayi Zhang, Tong Zhang, Qianyu Tao, Youlang Zhou, Limin Ma, Yangbo Guan

{"title":"SiRNA-Targeting TGF-β1 Based on Nanoparticle-Coated Ureteral Stents to Inhibit Ureteral Stricture.","authors":"Wei Meng, Ningning Li, Feng Lv, Bo Chen, Shuaijiang Lu, Jiayi Zhang, Tong Zhang, Qianyu Tao, Youlang Zhou, Limin Ma, Yangbo Guan","doi":"10.1021/acsbiomaterials.4c01925","DOIUrl":"10.1021/acsbiomaterials.4c01925","url":null,"abstract":"Ureteral stricture is a difficult urological problem with no optimal solution and is the result of scar hyperplasia and fibrosis caused by ureteral injury. Preventing the formation of ureteral strictures around drug-loaded ureteral stents is at the heart of the current research. TGF-β1 is a key factor affecting collagen deposition and fiber formation. Therefore, in this study, we established a rabbit ureteral stricture model, implanted a ureteral stent loaded with TGF-β1-siRNA for treatment, and compared the histopathology of ureteral stricture and the protein expression of genes related to the formation of stricture between different groups to test their therapeutic effects. We used sustained- and slow-release properties of the nanoparticles that were confirmed through in vitro experiments. The results of the fluorescence immunoassay showed that siRNA loaded by ureteral stents had high transfection efficiency on human ureter epithelial cells in vivo. In addition, the rabbit ureteral stricture model experiment verified that TGF-β1-siRNA could effectively transfect into ureteral tissues and inhibit the expression of TGF-β1, thereby inhibiting ureteral stricture. At the same time, the images of rabbit gross anatomy specimens showed that the hydronephrosis could also be effectively relieved. In summary, all the results mentioned above suggest that ureteral stents combined with RNA interference technology and a nanoparticle delivery system have broad prospects for clinical application in the suppression of ureteral stricture.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3477-3487"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electric Stimulation Combined with Biomaterials for Repairing Spinal Cord Injury. 电刺激联合生物材料修复脊髓损伤。

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-06-09 Epub Date: 2025-05-22 DOI: 10.1021/acsbiomaterials.5c00615

Lulu Du, Liya Zhang, Shengzhe Bao, Fangsu Yan, Wenwei Jiang, Hui Wang, Chuanming Dong

{"title":"Electric Stimulation Combined with Biomaterials for Repairing Spinal Cord Injury.","authors":"Lulu Du, Liya Zhang, Shengzhe Bao, Fangsu Yan, Wenwei Jiang, Hui Wang, Chuanming Dong","doi":"10.1021/acsbiomaterials.5c00615","DOIUrl":"10.1021/acsbiomaterials.5c00615","url":null,"abstract":"Spinal cord injury (SCI) is a central nervous system (CNS) disease with a high disability rate, and reconstructing motor function after SCI remains a global challenge. Recent advancements in rehabilitation and regenerative medicine offer new approaches to SCI repair. Electrical stimulation has been shown to alter cell membrane charge distribution, generating action potentials, and affecting cell behavior. This method aids axon regeneration and neurotrophic factor upregulation, crucial for nerve repair. Biomaterials, used as scaffolds or coatings in cell culture and tissue engineering, enhance cell proliferation, migration, differentiation, and tissue regeneration. Electroactive biomaterials combined with electrical stimulation show promise in regenerating nerve, heart, and bone tissues. In this paper, different types of electrical stimulation and biomaterials applied to SCI are described, and the current application and research progress of electrical stimulation combined with biomaterials in the treatment of SCI are described, as well as the future prospects and challenges.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"3276-3296"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 5.4 2区医学