ACS Biomaterials Science & Engineering最新文献_第8页

Natural Biogenic Templates for Nanomaterial Synthesis: Advances, Applications, and Environmental Perspectives.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-10 DOI: 10.1021/acsbiomaterials.4c02075

Srujana T L, K Jagajjanani Rao, Tarangini Korumilli

{"title":"Natural Biogenic Templates for Nanomaterial Synthesis: Advances, Applications, and Environmental Perspectives.","authors":"Srujana T L, K Jagajjanani Rao, Tarangini Korumilli","doi":"10.1021/acsbiomaterials.4c02075","DOIUrl":"10.1021/acsbiomaterials.4c02075","url":null,"abstract":"This review explores the use of biogenic templates in nanomaterial synthesis, emphasizing their role in promoting environmentally sustainable nanotechnology. It categorizes various biogenic templates, including agricultural byproducts and microorganisms, stating their suitability for forming nanostructures due to their distinct properties. A comparative analysis of monostep and multistep synthesis methods is provided, focusing on their efficiencies and outcomes when using biogenic templates. Further, this review also highlights how these templates can generate complex nanostructures and hybrid materials with enhanced functionalities. Applications of biogenic templates across biomedicine, biotechnology, environmental science, and energy are discussed along with their utilization scope in agriculture and electronics. Benefits from nanostructures from biotemplates include sustainability, low cost, and reduced toxicity, but challenges like scalability, reproducibility, and regulatory compliance persist. Future research focuses on improving synthesis techniques, discovering new templates, and evaluating environmental and cytotoxic impacts, especially for biomedical uses. In conclusion, the review reaffirms the potential of biogenic templates in sustainable nanomaterial synthesis while highlighting the ongoing challenges that need to be addressed for broader adoption.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1291-1316"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of the Loading-Unloading Cycles on the Tensile Behavior and Structures of Spider Tubular Gland Silk.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-17 DOI: 10.1021/acsbiomaterials.4c01608

Yi-Qin Hong, Xin-Ru Zhang, Li-Hua Wu, Tai-Yong Lv, Xin-Jun Liao, Gustavo V Guinea, José Pérez-Rigueiro, Ping Jiang

{"title":"The Effect of the Loading-Unloading Cycles on the Tensile Behavior and Structures of Spider Tubular Gland Silk.","authors":"Yi-Qin Hong, Xin-Ru Zhang, Li-Hua Wu, Tai-Yong Lv, Xin-Jun Liao, Gustavo V Guinea, José Pérez-Rigueiro, Ping Jiang","doi":"10.1021/acsbiomaterials.4c01608","DOIUrl":"10.1021/acsbiomaterials.4c01608","url":null,"abstract":"There exists a significant correlation between the microstructural evolution and the mechanical properties of fibers during repeated loading and unloading cycles. Nevertheless, the influence of deformation and the duration of intervals on the structural and tensile behavior of spider silk after repeated stretching at a given strain value has been rarely reported, with the exception of studies focusing on the major ampullate gland silk (Mas) of the spider. In order to investigate the effects of repeated stretching on the structural and mechanical behavior of spider tubular gland silk (Tus), the tensile properties and the changes in semiquantitative protein secondary structure of Argiope bruennichi Tus during loading-unloading cycles were characterized. The results indicate that the typical tensile behavior curves of Tus were irreversibly modified to resemble those of Mas, demonstrating a clear yield region accompanied by a necking phenomenon. The Tus displays remarkable characteristics of repeated stretching and mechanical memory, and it is capable of reproducing the tensile behavior of fibers subjected to one stretch, independent from its previous loading history. The above phenomenon may be caused by repeated stretching leading to the damage and reconstruction of protein structures, including an increase in α-helix content and the rearrangement of spider-silk proteins, enabling them to reproduce their mechanical behavior. These findings may provide valuable insights for the biomimetic design of novel fiber materials, such as the spider silk gut, through the artificial stretching of spider silk glands.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1379-1390"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bacteria-Targeting Supramolecular Nanophotosensitizer for Combating Multidrug Resistant Bacteria.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-17 DOI: 10.1021/acsbiomaterials.4c02047

Wenlong Huang, Anan Wang, Wenchen Wang, Lihong Lin, Jianyu Rong, Jia Tian, Weian Zhang

{"title":"A Bacteria-Targeting Supramolecular Nanophotosensitizer for Combating Multidrug Resistant Bacteria.","authors":"Wenlong Huang, Anan Wang, Wenchen Wang, Lihong Lin, Jianyu Rong, Jia Tian, Weian Zhang","doi":"10.1021/acsbiomaterials.4c02047","DOIUrl":"10.1021/acsbiomaterials.4c02047","url":null,"abstract":"The increasing prevalence of multidrug-resistant bacteria is a significant global health threat. In contrast to conventional antibiotic treatments, photodynamic therapy (PDT) offers a promising alternative by reducing the bacterial adaptability to antibiotics and bactericides. However, traditional photosensitizers encounter poor antimicrobial efficacy due to poor hydrophilicity of photosensitizers, short lifespan, narrow diffusion radius of reactive oxygen species (ROS), and the risk of exacerbating inflammation. In this study, we report a bacterial-targeting supramolecular nanophotosensitizer for combating multidrug resistant bacteria. The nanophotosensitizer, formed through host-guest interactions and self-assembly of tetra-cyclodextrin-modified silver porphyrin (AgTPP-CD4), adamantyl-modified phenylboronic acid (Ad-PBA), and curcumin (Cur), can effectively target and kill methicillin-resistant Staphylococcus aureus (MRSA). Moreover, it reduces inflammation and promotes wound healing in MRSA-infected wounds without inducing drug resistance. The combination of supramolecular chemistry and targeted PDT offers a promising strategy for combating multidrug-resistant bacterial infections.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1741-1750"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Step Fabrication of V-Shaped Polymeric Microwells to Enhance Cancer Spheroid Formation.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-18 DOI: 10.1021/acsbiomaterials.4c02359

Omar M Rahman, Roberto Tarantino, Stephen D Waldman, Dae Kun Hwang

{"title":"Single-Step Fabrication of V-Shaped Polymeric Microwells to Enhance Cancer Spheroid Formation.","authors":"Omar M Rahman, Roberto Tarantino, Stephen D Waldman, Dae Kun Hwang","doi":"10.1021/acsbiomaterials.4c02359","DOIUrl":"10.1021/acsbiomaterials.4c02359","url":null,"abstract":"Traditional cancer research has long relied on two-dimensional (2D) cell cultures, which inadequately mimic the complex three-dimensional (3D) microenvironments of in vivo tumors. Recent advancements in 3D cell cultures, particularly cancer spheroids, have highlighted their superior physiological relevance. However, existing methods for spheroid generation often require complex, multistep fabrication processes that limit scalability and reproducibility. In this study, we present a novel single-step photolithographic technique to fabricate high-aspect-ratio V-slanted hydrogel microwells. By employing polyethylene glycol (PEG)-based hydrogels, we create biocompatible, extracellular matrix (ECM)-like scaffolds that enhance gas and nutrient exchange while promoting uniform spheroid formation. The hydrogel microwells allow precise control of spheroid size, achieving a physiologically relevant diameter of 425 μm within 12-24 h, and the resulting spheroids exhibiting high viability over 3 weeks. Moreover, the method facilitates the creation of scalable multiwell arrays for high-throughput applications, making it suitable for both small-scale and large-scale experimental needs. This platform addresses the limitations of traditional microwell fabrication, offering a robust, efficient, and reproducible system for generating physiologically relevant 3D models with valuable applications in cancer research, drug testing, and tissue engineering.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1857-1868"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cu_2-xO@Ti₃C₂ Integrated Photothermal Nanofibers with Antibacterial, Anti-Inflammatory, and Hemostatic Properties for Promoting Infected Diabetic Wound Healing.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-22 DOI: 10.1021/acsbiomaterials.4c01936

Tao Liao, Jinxiu Liu, Jia Chen, Zhongjia Liu, Guolie Xie, Ning Guo, Ying Kuang, Linghui Dian, Cao Li, Yun Liu

{"title":"Cu2-xO@Ti3C2 Integrated Photothermal Nanofibers with Antibacterial, Anti-Inflammatory, and Hemostatic Properties for Promoting Infected Diabetic Wound Healing.","authors":"Tao Liao, Jinxiu Liu, Jia Chen, Zhongjia Liu, Guolie Xie, Ning Guo, Ying Kuang, Linghui Dian, Cao Li, Yun Liu","doi":"10.1021/acsbiomaterials.4c01936","DOIUrl":"10.1021/acsbiomaterials.4c01936","url":null,"abstract":"Infected diabetic wounds represent a significant challenge in clinical care due to persistent inflammation and impaired healing. To address these issues, the development of novel wound dressings with both antibacterial and reactive oxygen species (ROS) scavenging properties is essential. Herein, we prepare a novel wound dressing composed of Cu2-xO nanoparticles decorated on Ti3C2 MXene (Cu2-xO@Ti3C2) and integrate it into a poly(vinyl alcohol) (PVA) matrix to form electrospun nanofibers (Cu2-xO@Ti3C2@PVA). Cu2-xO@Ti3C2 exhibits remarkable photothermal conversion efficiency and effective ROS scavenging properties. In vitro experiments demonstrated that Cu2-xO@Ti3C2 effectively kills bacteria upon near-infrared (NIR) irradiation, which can be attributed to the photothermal therapy (PTT) effect of Ti3C2. At the same time, the ROS scavenging abilities of both Ti3C2 and Cu2-xO endow Cu2-xO@Ti3C2 with significant in vitro anti-inflammatory effects. As a promising wound dressing, in vivo studies validated the high efficacy of Cu2-xO@Ti3C2@PVA in promoting hemostasis, exerting antibacterial activity, reducing inflammation, and accelerating the healing process of diabetic wounds. This innovative approach provides a comprehensive solution to the multifaceted challenges of diabetic wound healing and paves the way for improved clinical outcomes.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1675-1689"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microstructural and Micromechanical Properties of Decellularized Fibrocartilaginous Scaffold.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-23 DOI: 10.1021/acsbiomaterials.4c01195

Jie Zhang, Liang Xie, Yilin She, Han Luo, Songsong Zhu, Nan Jiang

{"title":"Microstructural and Micromechanical Properties of Decellularized Fibrocartilaginous Scaffold.","authors":"Jie Zhang, Liang Xie, Yilin She, Han Luo, Songsong Zhu, Nan Jiang","doi":"10.1021/acsbiomaterials.4c01195","DOIUrl":"10.1021/acsbiomaterials.4c01195","url":null,"abstract":"Fibrocartilage decellularized extracellular matrix (dECM) is a promising alternative material for damaged fibrocartilage repair and replacement due to its biomimetic gross morphology and internal microstructure. However, the alterations in the microstructure and micromechanical properties of fibrocartilage after decellularization interfere with the macroscopic functional application of the scaffold. Therefore, this study aims to present an analytical atlas of the microstructure and micromechanics of the fibrocartilaginous dECM scaffold to elucidate the effect of decellularization treatment on the macroscopic function of the scaffold. The fibrocartilage dECM was prepared using the temporomandibular joint (TMJ) disc as the model, and its durability was evaluated under three functional states (physiological, physiological limit, and beyond the limit). The macroscopic function of different fibrocartilage dECM exhibits notable differences, which are attributed to the destruction of the multilevel collagen structure. This process involves unwinding triple-helix tropocollagen molecules, destroying collagen fibril D-periodicity, expanding collagen fiber bundle curling, and loosening of the collagen fiber network. The impairment of multiscale collagen structures degrades the cross-scale mechanical modulus and energy dissipation of dECM from the triple helix molecules to the fibril level to the fiber bundle that extends to the fiber network. This study provides important data for further optimizing decellularized fibrocartilage scaffolds and evaluating their translational potential.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1562-1570"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic Silk Nanoparticle Manufacture: Calcium Ion-Mediated Assembly.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-01-30 DOI: 10.1021/acsbiomaterials.4c02175

Napaporn Roamcharern, Saphia A L Matthew, Daniel J Brady, John A Parkinson, Zahra Rattray, F Philipp Seib

{"title":"Biomimetic Silk Nanoparticle Manufacture: Calcium Ion-Mediated Assembly.","authors":"Napaporn Roamcharern, Saphia A L Matthew, Daniel J Brady, John A Parkinson, Zahra Rattray, F Philipp Seib","doi":"10.1021/acsbiomaterials.4c02175","DOIUrl":"10.1021/acsbiomaterials.4c02175","url":null,"abstract":"Silk has emerged as an interesting candidate among protein-based nanocarriers due to its favorable properties, including biocompatibility and a broad spectrum of processing options to tune particle critical quality attributes. The silk protein conformation during storage in the middle silk gland of the silkworm is modulated by various factors, including the most abundant metallic ion, calcium ion (Ca2+). Here, we report spiking of liquid silk with calcium ions to modulate the silk nanoparticle size. Conformational and structural analyses of silk demonstrated Ca2+-induced silk assemblies that resulted in a liquid crystalline-like state, with the subsequent generation of β-sheet-enriched silk nanoparticles. Thioflavin T studies demonstrated that Ca2+ effectively induces self-assembly and conformation changes that also increased model drug loading. Ca2+ incorporation in the biopolymer feed significantly increased the nanoparticle production yield from 16 to 89%, while simultaneously enabling Ca2+ concentration-dependent particle-size tuning with a narrow polydispersity index and altered zeta potential. The resulting silk nanoparticles displayed high biocompatibility in macrophages with baseline levels of cytotoxicity and cellular inflammation. Our strategy for manufacturing biomimetic silk nanoparticles enabled overall tuning of particle size and improved yields─features that are critical for particle-based nanomedicines.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"1847-1856"},"PeriodicalIF":5.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-10 Epub Date: 2025-02-12 DOI: 10.1021/acsbiomaterials.4c02005

Tristan M Nicholson, Jodie C Tokihiro, Wan-Chen Tu, Jian Wei Khor, Ulri N Lee, Erwin Berthier, John K Amory, Thomas J Walsh, Charles H Muller, Ashleigh B Theberge

引用次数: 0

Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-07 DOI: 10.1021/acsbiomaterials.4c02399

Benedikt Gantert, Emine Karakaya, Florian Hofmann, Tomasz Jungst, Lorenz Meinel, Anja K Bosserhoff, Rainer Detsch, Tessa Lühmann

{"title":"Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells.","authors":"Benedikt Gantert, Emine Karakaya, Florian Hofmann, Tomasz Jungst, Lorenz Meinel, Anja K Bosserhoff, Rainer Detsch, Tessa Lühmann","doi":"10.1021/acsbiomaterials.4c02399","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02399","url":null,"abstract":"Biofabrication and three-dimensional (3D) bioprinting enable precise spatial arrangement of cells within biomaterial scaffolds. We developed an alginate-based and Förster resonance energy transfer (FRET)-responsive \"turn-on\" reporter ink platform to enable real-time monitoring of matrix metalloproteinase (MMP) activity. Three distinct MMP-cleavable turn-on peptide reporters were synthesized and characterized for their cell-specific cleavage profiles using recombinant MMPs, cell-derived media, and different cell cultures (NIH3T3, HEK293, and MelHo). All turn-on reporters were covalently and site-specifically incorporated into alginate dialdehyde (ADA) to yield an MMP reporter ink. The ADA reporter ink with an MMP 13 turn-on reporter was responsive to all tested cell types over time within the cast bulk constructs. The ADA reporter ink material blended with gelatin had comparable print resolution and structural fidelity as observed for ADA. The extrusion-based bioprinted MelHo cell grids, measuring 2 × 2 cm2 and containing 1 × 106 cells/mL, exhibited MMP activity responses comparable to those of the casted reporter ink system, with a 3-fold increase observed at 24 h. This study introduces a versatile, FRET-based alginate bioink platform for the real-time monitoring of MMP activities, expanding the toolkit to understand cellular performance in bioprinted 3D constructs.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalized Poly(ethylene Glycol) Diacrylate Scaffolds for In Situ Immunomodulation of Dendritic Cells Targeting Melanoma Tumor.

IF 5.4 2区医学

ACS Biomaterials Science & Engineering Pub Date : 2025-03-06 DOI: 10.1021/acsbiomaterials.4c02036

Neha Dalal, Hemavathi Dhandapani, Arvind Ingle, Deepak Sharma, Prakriti Tayalia

{"title":"Functionalized Poly(ethylene Glycol) Diacrylate Scaffolds for In Situ Immunomodulation of Dendritic Cells Targeting Melanoma Tumor.","authors":"Neha Dalal, Hemavathi Dhandapani, Arvind Ingle, Deepak Sharma, Prakriti Tayalia","doi":"10.1021/acsbiomaterials.4c02036","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02036","url":null,"abstract":"Various immunotherapeutic strategies are being developed to fight cancer, which is one of the leading causes of mortality. Dendritic cells (DCs), being professional antigen-presenting cells, after efficient manipulation with tumor-associated antigens, can lead to effective T-cell recruitment and activation at the tumor site, resulting in cytotoxic T-cell-mediated cancer cell killing. To circumvent the inefficiencies of ex vivo DC modification and patient infusion, an alternative strategy involving in situ DC activation has been explored here. Here, the vaccine components are tumor lysates, as antigens, and polyinosinic:polycytidylic acid (poly(I:C)), a toll-like receptor-3 (TLR3) agonist, as an adjuvant. Our in vitro studies demonstrate that complexing poly(I:C) with a carrier molecule, chitosan, enhances its stability and accessibility to TLR3 in the DC endosomal membrane. Material-based localized delivery of immunomodulatory factors is known to improve their stability and reduce their off-target side effects. Here, PEGDA-PLL-based macroporous scaffolds allow easy recruitment of host cells, thereby enabling effective interaction between the vaccine components loaded on them and the infiltrating immune cells. The vaccine components present in the scaffold facilitate efficient DC activation and migration, leading to subsequent T-cell activation and antitumor response, as shown by our in vivo studies.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0