ACS Biomaterials Science & Engineering最新文献

{"title":"Novel Biomimetic Collagen-Based Corneal Repair Material Achieved via a \"Killing Two Birds with One Stone\" Strategy Using Carboxymethyl-β-Cyclodextrin.","authors":"Kuan Cheng, Xiaohong Chen, Yifan Yi, Yue Wang, Mengdie Tian, Jingjing Yu, Yuxin Xia, Jingyi Li, Min Zhang, Cuicui Ding","doi":"10.1021/acsbiomaterials.4c02203","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02203","url":null,"abstract":"<p><p>Collagen, as the principal structural component of the cornea, has emerged as a promising biomaterial for artificial corneal owing to its excellent biocompatibility and degradability. However, the mechanical properties of current collagen membrane cannot match the requirements of artificial corneal materials. Inspired by the hierarchical lamellar organization of native corneal stromal collagen, a biomimetic collagen-based corneal repair material was designed via a \"killing two birds with one stone\" strategy. In this strategy, carboxymethyl-β-cyclodextrin (CM-β-CD) was incorporated into the collagen, serving dual functions: regulating the <i>in vitro</i> self-assembly process of collagen molecules and establishing multiple covalent cross-linking sites within the network. Concurrently, controlled external shear forces were applied to induce anisotropic alignment of collagen fibers, effectively replicating the highly organized structural hierarchy characteristic of native corneal stromal tissue. The resulting membrane exhibited a 67% enhancement in tensile strength (0.52 MPa) compared to pure collagen membranes. Notably, <i>in vivo</i> lamellar keratoplasty evaluations revealed accelerated tissue regeneration, achieving complete re-epithelialization within 14 days versus 28 days for controls. These findings establish the material's potential as an advanced artificial corneal for tissue engineering applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Noncanonical Disulfide Bond on Thermal Resistance and Binding Affinity of Shark-Derived Single-Domain Antibodies.","authors":"Shuo Qiu, Chang Liu, Guoqiang Li, Hong Lin, Limin Cao, Kaiqiang Wang, Xiudan Wang, Jianxin Sui","doi":"10.1021/acsbiomaterials.4c02215","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02215","url":null,"abstract":"<p><p>Single-domain antibodies (sdAbs) often exhibit superior thermal stability compared to traditional antibodies. Efforts are currently focused on enhancing their structural robustness and thermal refolding ability through protein engineering to achieve greater thermal properties and functionality in practical applications. Thermal aggregation is a key factor hindering the reversible thermal denaturation of sdAbs. While studies have explored the role of noncanonical disulfide bonds in camelid-derived VHH aggregation, research on thermal aggregation in shark-derived sdAbs (also known as VNARs) remains scarce, limiting their potential for further optimization. In this study, the role of noncanonical disulfide bonds in VNAR structural robustness, aggregation, and affinity has been simultaneously investigated. Enzyme-linked immunosorbent assay (ELISA), circular dichroism, and intrinsic fluorescence were carried out to compare thermal antigen-binding stability, refolding abilities, and melting temperatures of four wild VNARs B7, 1N9, 2E6, and 2E11 specific for different antigens. Meanwhile, nano differential scanning fluorimetry (nanoDSF) was applied, for the first time, to monitor the thermal aggregation of VNARs. Notably, 2E11, which lacked the noncanonical disulfide bond, demonstrated impressive performance in many aspects. When alanine mutation was engineered to remove the CDR1-CDR3 disulfide bond in 2E6, its refolding rate was increased, and thermal aggregation was prevented significantly. Furthermore, 2E6 exhibited enhanced thermal antigen-binding stability despite reduced structural robustness and affinity. This study provides deeper insights and theoretical support for improving VNAR biophysical properties, with potential applications in enhancing immunoassay performance.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Mirror-Image RNA Nanostructure for Enhanced Biostability and Drug Delivery Efficiency.","authors":"Ying Zhang, Yuliya Dantsu, Wen Zhang","doi":"10.1021/acsbiomaterials.4c02184","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02184","url":null,"abstract":"<p><p>The development of stable and efficient drug delivery systems is essential for advancing therapeutic applications. Here, we present an innovative approach using a mirror-image RNA (l-RNA) nanostructure to enhance the biostability and drug delivery efficiency. We engineered an l-RNA three-way junction structure conjugated with both small interfering RNA (siRNA) targeting MCL1 and the chemotherapeutic agent doxorubicin for targeted and synergistic drug delivery. This codelivery strategy leverages the combined effects of doxorubicin and MCL1 siRNA, achieving improved therapeutic outcomes. The l-RNA nanostructure demonstrates superior stability compared with natural d-RNA, resulting in reduced toxicity in healthy cells while maintaining therapeutic efficacy in cancer cells. This indicates that l-RNA nanostructures may offer enhanced biosafety when applied as therapeutic agents. The addition of folic acid (FA) to the nanostructure surface substantially increases both delivery specificity and endosomal escape efficiency, optimizing targeted delivery. Structural modeling also suggests a distinctive binding conformation of doxorubicin with l-DNA, setting it apart from native DNA interactions. This study highlights the potential of mirror-image nucleic acid nanostructures as robust and precise platforms for combinatorial drug delivery in cancer treatment.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-Assisted DNA SELEX: Dual-Site Targeting of a Single Protein.","authors":"Achut Prasad Silwal, Siddhartha Kalpa Samadhi Thennakoon, Raunak Jahan, Satya Prakash Arya, Rick Mason Postema, Hari Prasad Timilsina, Andrew Michael Reynolds, Kaytelee Brooke Kokensparger, Xiaohong Tan","doi":"10.1021/acsbiomaterials.4c02053","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02053","url":null,"abstract":"<p><p>Heterobivalent fusion aptamers that target a single protein show significant promise for studying protein-protein interactions. However, a major challenge is finding two distinct aptamers that can simultaneously recognize the same protein. In this study, we used a novel technique called Aptamer-Assisted DNA SELEX (AADS) to isolate two distinct aptamers capable of recognizing different sites on the programmed death-ligand 1 (PD-L1) protein. Initially, Aptamer 1 (P1C2) was identified by using conventional DNA SELEX targeting the PD-L1 protein. Subsequently, Aptamer 2 (P1CSC) was obtained via AADS, which was designed to bind to the PD-L1/P1C2 complex. After confirming that both aptamers could simultaneously recognize the PD-L1 protein, we engineered fusion aptamers by optimizing their orientation and linker sequences, resulting in the creation of the optimized fusion aptamer, P1CSC-T7-P1C1. Our fusion aptamer targeting PD-L1 demonstrated remarkable specificity and affinity, effectively inhibiting PD-1/PD-L1 interactions at both the protein and cellular levels. These findings highlight the potential of fusion aptamers via AADS as powerful tools for targeting the PD-L1 protein and cancer cells (A549 cells). This represents a significant advancement in aptamer-based molecular recognition and has the potential to drive innovation as a versatile method for targeting a wide range of proteins.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabrication of Tunable 3D Hydrogel for Investigating the Matrix Stiffness Impact on Breast Cancer Chemotherapy Resistance","authors":"Yue Chen,&nbsp;Dan Xue,&nbsp;Di Huang,&nbsp;Xinying Li,&nbsp;Yuyou Duan* and Bin Chen*,&nbsp;","doi":"10.1021/acsbiomaterials.4c0163610.1021/acsbiomaterials.4c01636","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01636https://doi.org/10.1021/acsbiomaterials.4c01636","url":null,"abstract":"<p >Matrix stiffness is a key factor in breast cancer progression, but its impact on cell function and response to treatment is not fully understood. Here, we developed a stiffness-tunable hydrogel-based three-dimensional system that recapitulates the extracellular matrix and physiological properties of human breast cancer in vitro. Adjusting the ratio of GelMA to PEGDA in the hydrogel formulation enabled the fine-tuning of matrix stiffness across a range of 7 to 52 kPa. Utilizing this three-dimensional (3D) hydrogel platform for a breast cancer cell culture has enabled precise functional evaluations. Variations in matrix stiffness resulted in significant changes in the morphology of breast cancer cells after 2 weeks of incubation. The analysis of transcriptomic sequencing revealed that the 3D microenvironment significantly changed the expression of a wide panel of transcriptomic profiles of breast cancer cells in various matrix stiffness. Gene Ontology analysis further suggested that specific biological functions could potentially be linked to the magnitude of the matrix stiffness. According to our findings, extracellular matrix rigidity modulates the sensitivity of breast cancer cells to paclitaxel and adriamycin. Notably, the expression of ABCB1 and YAP1 genes may be upregulated in the 3D culture environment, potentially contributing to the increased drug resistance observed in breast cancer cells. This work aims to establish facile adjustable hydrogels to deepen insights into matrix rigidity effects on breast cancer cells within 3D microenvironments, highlighting the critical role of extracellular matrix stiffness in modulating cell-matrix interactions.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1417–1431 1417–1431"},"PeriodicalIF":5.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility","authors":"Ramesh Singh,&nbsp;Liszt Y. C. Madruga,&nbsp;Aniruddha Savargaonkar,&nbsp;Alessandro F. Martins,&nbsp;Matt J. Kipper and Ketul C. Popat*,&nbsp;","doi":"10.1021/acsbiomaterials.4c0210610.1021/acsbiomaterials.4c02106","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02106https://doi.org/10.1021/acsbiomaterials.4c02106","url":null,"abstract":"<p >This study explores the synergistic effects of combining titania nanotubes (TiNTs) with the biopolymer Tanfloc (TAN) to enhance the surface properties of TiNTs for biomedical applications. We investigated the interactions of blood components and human adipose-derived stem cells (ADSCs) with TiNT surfaces covalently functionalized with Tanfloc (TAN), an aminolyzed polyphenolic tannin derivative. The functionalized surfaces (TiNT-TAN) have great potential to control protein adsorption and platelet adhesion and activation. Fluorescence and scanning electron microscopy (SEM) were used to analyze platelet adherence and activation. The amphoteric nature and multiple functional groups on TAN can control blood protein adsorption, platelet adhesion, and activation. Further, the modified surface supports adipose-derived stem cell (ADSC) viability, attachment, and growth without any cytotoxic effect. The TAN conjugation significantly (****<i>p</i> &lt; 0.0001) increased the proliferation rate of ADSCs compared to the TiNT surfaces.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1445–1455 1445–1455"},"PeriodicalIF":5.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsbiomaterials.4c02106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soy Protein-Cultured Mesenchymal Stem Cell-Secreted Extracellular Vesicles Target the Neurovascular Unit: Insights from a Zebrafish Brain Injury Model","authors":"Tai-I Lin,&nbsp;Pei-Ying Hsieh,&nbsp;Hui-Jen Lin,&nbsp;Cheng-Kang Chiang,&nbsp;Jim Jinn-Chyuan Sheu,&nbsp;Wei-Tien Chang,&nbsp;Ian Liau* and Hsin-Yun Hsu*,&nbsp;","doi":"10.1021/acsbiomaterials.4c0230410.1021/acsbiomaterials.4c02304","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02304https://doi.org/10.1021/acsbiomaterials.4c02304","url":null,"abstract":"<p >Cerebral vascular disorders often accompany hypoxia-induced brain injury. In this study, we develop a zebrafish model of hypoxia-induced cerebral vascular injury to replicate the associated phenotypic changes, including cerebrovascular damage, neuronal apoptosis, and neurological dysfunction. We then explored the therapeutic potential of extracellular vesicles derived from Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) cultured on soy protein-coated surfaces. These vesicles demonstrated superior recovery efficacy, especially in restoring the blood–brain barrier integrity and improving neurological function. Our findings suggest that these potent therapeutic extracellular vesicles, easily produced from WJ-MSCs cultured in the presence of soy proteins, may mitigate hypoxia-induced brain injury by decreasing the severity of vascular disorder caused by oxidative stress. Protein–protein interactome analysis further suggests that multiple signaling pathways are likely involved in restoring normal neurovascular unit function.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1432–1444 1432–1444"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsbiomaterials.4c02304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimuli-Responsive Self-Healing Ionic Gels: A Promising Approach for Dermal and Tissue Engineering Applications","authors":"Deepanjan Datta*,&nbsp;Viola Colaco,&nbsp;Sony Priyanka Bandi*,&nbsp;Namdev Dhas,&nbsp;Leela Sai Lokesh Janardhanam,&nbsp;Sudarshan Singh and Lalitkumar K. Vora,&nbsp;","doi":"10.1021/acsbiomaterials.4c0226410.1021/acsbiomaterials.4c02264","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02264https://doi.org/10.1021/acsbiomaterials.4c02264","url":null,"abstract":"<p >The rapid increase in the number of stimuli-responsive polymers, also known as smart polymers, has significantly advanced their applications in various fields. These polymers can respond to multiple stimuli, such as temperature, pH, solvent, ionic strength, light, and electrical and magnetic fields, making them highly valuable in both the academic and industrial sectors. Recent studies have focused on developing hydrogels with self-healing properties that can autonomously recover their structural integrity and mechanical properties after damage. These hydrogels, formed through dynamic covalent reactions, exhibit superior biocompatibility, mechanical strength, and responsiveness to stimuli, particularly pH changes. However, conventional hydrogels are limited by their weak and brittle nature. To address this, ionizable moieties within polyelectrolytes can be tuned to create ionically cross-linked hydrogels, leveraging natural polymers such as alginate, chitosan, hyaluronic acid, and cellulose. The integration of ionic liquids into these hydrogels enhances their mechanical properties and conductivity, positioning them as significant self-healing agents. This review focuses on the emerging field of stimuli-responsive ionic-based hydrogels and explores their potential in dermal applications and tissue engineering.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1338–1372 1338–1372"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsbiomaterials.4c02264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrier-Free Cisplatin–Dactolisib Nanoparticles for Enhanced Synergistic Antitumor Efficacy","authors":"Mei Zhang,&nbsp;Qiuxia Tan,&nbsp;Sevil Gonca,&nbsp;Minhuan Lan*,&nbsp;Bin-Zhi Qian,&nbsp;Xianfeng Chen* and Norbert Radacsi,&nbsp;","doi":"10.1021/acsbiomaterials.4c0067210.1021/acsbiomaterials.4c00672","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c00672https://doi.org/10.1021/acsbiomaterials.4c00672","url":null,"abstract":"<p >Cisplatin (CDDP) is one of the most commonly used chemotherapeutic agents for solid tumors and hematologic malignancy. However, its therapeutic outcomes have remained unsatisfactory due to severe side effects, a short elimination half-life, the emergence of drug resistance, and the induction of metastasis. Combination with other chemotherapeutic agents has been proposed as one strategy to address the drawbacks of CDDP-based therapy. Therefore, this study aimed to boost the antitumor efficacy of cisplatin (CDDP) with a PI3K/mTOR dual inhibitor, dactolisib (BEZ), via a carrier-free codelivery system based on the self-assembly of the coordinated CDDP–BEZ. The synthesized CDDP–BEZ nanoparticles (NPs) possess sensitive pH-responsiveness, facilitating the delivery of both drugs to cancer cells. CDDP–BEZ NPs specifically enhanced cytotoxicity in cancer cells due to the synergy between cisplatin and dactolisib, resulting in augmented DNA damage, activation of mitochondria-dependent apoptosis, and increased inhibition on the PI3K/mTOR signaling axis. The inhibition of tumor migration and metastasis by CDDP–BEZ NPs was observed both in vitro and in vivo. Our data suggest that CDDP–BEZ NPs could serve as a safe and effective platform to maximize the synergy between both drugs in combating cancer, presenting a strategy to promote the therapeutic efficacy of platinum-based chemotherapeutic agents by combining them with PI3K inhibitors.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1456–1471 1456–1471"},"PeriodicalIF":5.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsbiomaterials.4c00672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing Positionally Stable Smooth Breast Implants","authors":"Tim Y. Li,&nbsp;Sophia Salingaros,&nbsp;Hector F. Salazar,&nbsp;Riley D. Mayne,&nbsp;Jini Jeon,&nbsp;Carlos Urrea de la Puerta,&nbsp;Matthew W. Liao,&nbsp;Samuel J. Medina,&nbsp;Xue Dong,&nbsp;Lawrence J. Bonassar and Jason A. Spector*,&nbsp;","doi":"10.1021/acsbiomaterials.4c0222210.1021/acsbiomaterials.4c02222","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02222https://doi.org/10.1021/acsbiomaterials.4c02222","url":null,"abstract":"<p ><i>Background</i>: The voluntary recall and ban of several textured breast implant models worldwide, secondary to their association with Breast Implant-Associated Anaplastic Large Cell Lymphoma, has limited the key benefit of a textured surface─positional stability. We have engineered a Positionally Stable Smooth Implant (PSSI) containing millimeter-scaled cylindrical wells on the implant surface for capsule ingrowth and device stabilization. <i>Objectives</i>: To evaluate the long-term positional stability of PSSI designs <i>in vivo</i> and characterize capsule formation. <i>Methods</i>: Miniature breast implants were manufactured using poly(dimethylsiloxane). PSSI were designed with various dimensions of well width, depth, and number. Comparison groups consisted of smooth and textured implants. Six sterilized implants per group were implanted subcutaneously into the bilateral dorsa of Sprague–Dawley rats. Implant rotation was measured with MicroCT every 2 weeks. Implant-capsule units were explanted at 3 months for histological analysis. <i>Results</i>: All PSSI groups exhibited significantly less cumulative positional rotation than smooth implants (<i>p</i> &lt; 0.05), with stability comparable to that of textured implants. Upon explantation, microCT and gross examination revealed notable capsule ingrowth within the PSSI wells. Histological evaluation of foreign body response showed significantly fewer pro-inflammatory M1 macrophages in the PSSI capsules compared to the textured control. Additionally, myofibroblast expression, which is implicated in capsular contracture, was significantly lower in both the PSSI and textured groups compared to smooth implants. <i>Conclusions</i>: This novel smooth-surface breast implant design provided equivalent positional stability and reduced pro-inflammatory M1 macrophage expression compared to textured implants. These results suggest a promising, safer alternative to textured implants for inducing positional stability.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 3","pages":"1765–1775 1765–1775"},"PeriodicalIF":5.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}