ACS Biomaterials Science & Engineering最新文献

{"title":"Integration of 3% Silver-Doped Hydroxyapatite Coated on Alumina Using Radio Frequency Magnetron Sputtering for Superior Bioactivity, Mechanical and Electrical Properties, and Wear Resistance.","authors":"Ranbir Kumar, Deep Shikha, Smit Anand, Sanjay Kumar Sinha, Paresh Kumar Mohanty, Sanjay Mhaske, Abhinandan Kumar, Arkadeb Mukhopadhyay","doi":"10.1021/acsbiomaterials.4c02471","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02471","url":null,"abstract":"<p><p>The research investigates the integration of 3% silver-doped hydroxyapatite (Ag-HAP) onto a hexagonal alumina substrate with a matching structure to reduce interface strain utilizing radio frequency magnetron sputtering (RFMS). This method aims to improve film adhesion while enhancing the bioactivity, antimicrobial properties, and wear resistance of biomedical implants. Hydroxyapatite (HAP) has excellent biocompatibility and is widely used in bone implants due to its similarity to bone minerals, but it suffers from brittleness and limited mechanical strength. By doping Ag with HAP, mechanical and antimicrobial properties are enhanced, addressing infection and material longevity challenges. Alumina (Al₂O₃) is known for its mechanical strength and wear resistance, making it a suitable substrate for implants; however, its lack of bioactivity requires modification. The RFMS technique ensures a uniform and well-adhered nanocoating of Ag-HAP on alumina, creating a composite material that balances alumina's durability with silver-doped HAP bioactivity and antimicrobial benefits. The study reveals improved mechanical properties, such as increased hardness and wear resistance, along with enhanced antibacterial efficacy, making the composite material promising for orthopedic applications. The characterization of coatings using various analytical techniques such as EDS, FESEM, FTIR, and XRD confirms the formation and stability of Ag-HAP, while electrical properties are described by dielectric measurements. The changes in the lattice parameters, grain size, and pore size led to changes in hardness, coefficient of friction, and ultimately, the material's biocompatibility. Improvement in corrosion resistance after coating can be due to intermetallic compound formation at the interface. Biocompatibility was studied through assays that show favorable results, supporting the potential of Ag-HAP/Al₂O₃ in implantology. The mechanism of improvement in the antibacterial mechanism against <i>E. coli</i> and <i>S. aureus</i> is proposed. This research proposes a novel solution to implant-related challenges by combining silver-doped hydroxyapatite mechanical and biological advantages with alumina, thereby optimizing both biocompatibility and structural integrity for long-term use in biomedical implants.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2622-2638"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Theraoenergetics for Cartilage Regeneration: Development of a Therapeutic and Bioenergetic Loaded Janus Nanofiber Reinforced Hydrogel Composite for Cartilage Regeneration.","authors":"Shivani Chaudhary, Sakshi Jain, Doyel Ghosal, Sachin Kumar","doi":"10.1021/acsbiomaterials.4c01600","DOIUrl":"10.1021/acsbiomaterials.4c01600","url":null,"abstract":"<p><p>Advancements in tissue engineering and regenerative medicine have highlighted different strategies of engineering and designing hydrogels to replicate the intricate structure of cartilage extracellular matrix (ECM) for effective cartilage regeneration. However, despite efforts to meet the elevated structural and mechanical demands of cartilage repair, researchers often overlook the challenging environmental conditions at damaged cartilage sites such as inflammation, hypoxia, and the limited availability of nutrients and energy, which are critical for supporting tissue regeneration. The insufficient oxygen, nutrient availability, and oxidative stress in avascular cartilage limit the oxidative phosphorylation-mediated bioenergetics in cells needed for energy demands required for anabolic biosynthesis, cell division, and migration during tissue repair. Thus, there is a need to develop an advanced approach to engineer a unique hydrogel system that not only provides intricate structural properties but also integrates therapeutics (like anti-inflammatory, reactive oxygen species (ROS) scavenging) and bioenergetics (like oxygen, energy demand) into the hydrogel, which may offer a holistic and effective solution for repairing cartilage defects under a harsh microenvironment. In this study, we engineered an innovative approach to develop a new class of theraoenergetic hydrogel system by reinforcing a Janus nanofiber (JNF) carrying therapeutic (MgO) and bioenergetic (polyglutamic acid), PGA) components into a dual network photo-crosslinkable hydrogel. Reinforcement of JNF microfragments and the photo-crosslinking dual network of synthesized gelatin methacryloyl (GelMA) and carboxymethyl chitosan (CMCh) not only enhances the hydrogel's mechanical properties by 800% to withstand mechanical load but also ensures a controlled release of magnesium, oxygen, and PGA over 30 days. Co-delivery of magnesium and bioenergetic PGA with oxygen helped synergistically to reduce intracellular ROS and inflammatory markers IL-6 and TNF-α, providing a supportive environment for enhancing cell mitochondrial oxidative metabolism leading to active proliferation and chondrogenic differentiation of stem cells to deposit glycosaminoglycan (GAG)-rich extracellular matrix to regenerate cartilage. The developed theraoenergetic hydrogel system represents a promising solution for regenerating cartilage under a harsh microenvironment to treat osteoarthritis, a rising global health burden.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2792-2809"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of Metal-Polyphenol Self-Assembled Nanoparticles: Synthesis, Properties, and Biological Applications in Inflammatory Diseases.","authors":"Li Wan, Shizhe Li, Jiawei Du, Anqi Li, Yujie Zhan, Wufu Zhu, Pengwu Zheng, Dan Qiao, Cunpeng Nie, Qingshan Pan","doi":"10.1021/acsbiomaterials.4c02366","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02366","url":null,"abstract":"<p><p>Polyphenols, which are compounds characterized by the presence of phenolic hydroxyl groups, are abundantly found in natural plants and exist in highly complex forms within living organisms. As some of the most prevalent compounds in nature, polyphenols possess significant medicinal value due to their unique structural features, particularly their therapeutic efficacy in antitumor, anti-inflammatory, and antibacterial applications. In the context of inflammation therapy, polyphenolic compounds can inhibit the excessive release of inflammatory mediators from inflammatory cells, thereby mitigating inflammation. Furthermore, these compounds exhibit strong antioxidant properties, enabling them to scavenge free radicals and reactive oxygen species (ROS), reduce oxidative stress-related damage, and exert anti-inflammatory effects. Due to their multiple phenolic hydroxyl groups and their ability to chelate various metals, polyphenols are extensively utilized in the synthesis of self-assembled nanoparticles for the treatment of various diseases. Numerous studies have demonstrated that the therapeutic profile of nanoparticles formed through self-assembly with metal ions surpasses that of polyphenolic compounds alone. This Review will focus on the self-assembly of different polyphenolic compounds with various metal ions to generate nanoparticles, their characterization, and their therapeutic applications in inflammation-related diseases, providing researchers with new insights into the synthetic study of metal-polyphenol nanocomposites and their biological applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2502-2527"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Targeted Drug Delivery System to Control Avidity and Drug Encapsulation Using E2 Nanocages and SpyTag/SpyCatcher.","authors":"Dohee Ahn, Sun Hee Park, Yeong Geun Lee, Myeong Seon Jeong, Geetanjali B Gone, Younghun Cho, Sang J Chung","doi":"10.1021/acsbiomaterials.5c00224","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00224","url":null,"abstract":"<p><p>Although antibody-drug conjugates offer advanced targeted anticancer therapy that overcomes the limitations of conventional chemotherapy and therapeutic antibodies, they are restricted in their capacity to carry multiple hydrophobic payloads. Protein nanocages have emerged as versatile therapeutic platforms for targeted drug delivery, offering advantages like precise molecular assembly, biocompatibility, and multivalent targeting. This study presents the development of engineered E2 nanocages functionalized with anti-HER2 single-chain variable fragments (scFv) using the SpyTag/SpyCatcher ligation system to achieve controlled scFv display valency. The results demonstrate that increasing anti-HER2 scFv valency enhances HER2 binding affinity via avidity effects, with the highest valency nanocages showing the highest binding avidity. Furthermore, cysteine residues were introduced into the E2 nanocages to enable conjugation with monomethyl auristatin E (MMAE) through maleimide chemistry, achieving efficient drug loading. The resulting MMAE-conjugated nanocages displayed potent, subnanomolar cytotoxicity in HER2-positive SKBR3 and BT-474 cell lines while sparing HER2-negative MDA-MB-231 cells at concentrations up to 1 nM. These results underscore the critical role of scFv valency in enhancing HER2 targeting and highlight the potential of E2 protein nanocages as specific, potent platforms for targeted cancer therapy. In this study, we developed an enhanced targeted drug delivery system using E2 nanocages and scFv with SpyCatcher/SpyTag ligation to regulate binding avidity and encapsulate hydrophobic drugs. The modular design and pH-sensitive dissociation of these nanocages establish a foundation for next-generation precision medicine strategies.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2768-2782"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Renal Protection in Acute Kidney Injury with ROS-Activated Nanoparticles Targeting Oxidative Stress and Inflammation.","authors":"Tianyu Lan, Mei Li, Xiuheng Luo, Haijun Du, Xin Lu, Huijuan Mao, Honglei Guo, Qianqian Guo","doi":"10.1021/acsbiomaterials.4c01917","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c01917","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is often associated with oxidative stress, which leads to a range of pathological changes, including inflammation and cell apoptosis. These mechanisms highlight the crucial role of eliminating ROS in the pathogenesis of AKI. This study presented a ROS-activated drug delivery system, NPS_PBA@Hib, designed for the targeted delivery of the anti-inflammatory and antioxidant drug hibifolin (Hib) to the kidneys, marking its inaugural application in AKI therapy. The drug loading of Hib was up to be 15% by conversely binding with the phenylboronic acid parts in the nanoparticles. NPS_PBA@Hib increased cellular uptake of drugs in HK-2 cells and reduced oxidative stress-induced damage by scavenging ROS. The nanoparticles notably extended the retention of Hib in AKI kidneys when compared to healthy kidneys, leading to heightened accumulation in the renal tubules. NPS_PBA@Hib demonstrated Hib's reno-protective effects by reducing oxidative stress and inflammation. In essence, this research serves as the primary confirmation of Hib's efficacy in inhibiting NLRP3 signaling pathway for the AKI treatment. The findings suggest that NPS_PBA@Hib nanoparticles are effective in treating AKI, highlighting the promising potential of utilizing Hib as a natural antioxidant nanoplatform for AKI, as well as other ROS-related diseases.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2713-2726"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Probiotics with Low Oxygen Targeting <i>Porphyromonas gingivalis</i> and Gingival Fibroblasts for the Treatment of Periodontitis.","authors":"Shenghong Li, Zhibo Fan, Kaijun Zheng, Yujie Wu, Guannan Zhong, Xiaomei Xu","doi":"10.1021/acsbiomaterials.5c00111","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.5c00111","url":null,"abstract":"<p><p>The overuse of antibiotics has increased the prevalence of drug-resistant bacteria in periodontitis. \"Sentinel\" gingival fibroblasts, stimulated by pathogenic bacteria, continue to release signaling factors that affect stem cell repair and recruit immune cells, resulting in persistent inflammation in periodontal tissues, eventually leading to the loosening and loss of teeth. Periodontal pathogenic bacteria cause surface hypoxia, and gingival fibroblasts in the inflammatory microenvironment express HIF-1α, promoting hypoxic areas in periodontal pockets. No drug delivery system is available for the hypoxic region of periodontal pockets. We synthesized BI NPs via berberine (BBR) and indocyanine green (ICG) and formed BIP NPs by wrapping BI NPs with polydopamine (PDA), and the BIP NPs were delivered to the hypoxic region of the periodontal pocket by hitchhiking with the anaerobic probiotic <i>Bifidobacterium bifidum</i> (Bif). The BIP NPs released berberin (BBR) under near-infrared (NIR) irradiation, which inhibited the sulfur metabolism of <i>Porphyromonas gingivalis</i> via mild photothermal action and BBR-targeted serine acetyltransferase, resulting in a decrease in resistance to oxidative stress, thus exerting a nonantibiotic bacteriostatic effect. This mild photothermal effect facilitated the uptake of BIP NPs bygingival fibroblasts. Moreover, BBR targeted nuclear factor-erythroid 2-related factor 2 (NRF2) to reduce ferroptosis, and the gingival fibroblast supernatant modulated macrophage polarization through the NF-κB pathway. In the periodontitis rat model, Bif@BIP+NIR treatment carried the drug to deep periodontal pockets, decreasing local gingival ferroptosis and alleviating periodontitis symptoms. To summarize, engineered probiotics target low-oxygen periodontal pockets for drug delivery, <i>P. gingivalis</i> for nonantibiotic bacterial inhibition, and gingival fibroblasts to mitigate ferroptosis, thus alleviating periodontitis to reduce periodontitis.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2753-2767"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zein/ZnO-Modified 3D-Printed PCL/Sphene Scaffolds with Improved Bacterial Inhibition and Osteoblast Activity for Bone Regeneration Applications.","authors":"Monireh Kouhi, Mohammad Khodaei, Bahareh Behrouznejad, Omid Savabi, Mahdi Bodaghi","doi":"10.1021/acsbiomaterials.4c02193","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.4c02193","url":null,"abstract":"<p><p>3D printing offers a significant advantage in creating bioengineering scaffolds for patient-specific treatments of bony defects. In this study, a 3D-printed polycaprolactone (PCL)/sphene (SP, CaTiSiO5) scaffold coated with zein/ZnO was fabricated to provide a suitable environment for bone regeneration. SP nanoparticles were synthesized using a mechanochemical method and characterized by SEM-EDS, FTIR, and XRD. 0-30 wt % of prepared SP nanoparticles was used to fabricate 3D-printed PCL-based scaffolds. Incorporation of SP into PCL scaffolds (up to 20 wt %) significantly increased compressive strength (from 37.5 to 65.2 MPa) and modulus (from 0.33 to 0.63 MPa). <i>In vitro</i> bioactivity evaluation in simulated body fluid demonstrated the apatite formation ability of PCL/SP scaffolds, as confirmed by SEM-EDS analysis. Compared to PCL/SP, the zein/ZnO-modified scaffold showed increased surface hydrophilicity and significantly higher values of bactericidal potency against <i>S. aureus</i> and <i>E. coli</i>. Additionally, MTT assay, cell attachment, and alkaline phosphatase activity revealed that zein and ZnO coexistence on PCL/SP scaffolds resulted in significantly higher cell proliferation, improved cell adhesion, and enhanced osteogenic differentiation of MG-63 cells compared to unmodified samples. Overall, zein/ZnO-modified 3D-printed PCL/SP nanocomposite scaffolds with desirable physicochemical, mechanical, and biological characteristics can serve as superior platforms for bone regeneration applications.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2898-2909"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-Enabled Effective Tuberculosis Treatments: A Concise Overview.","authors":"Jingjing Li, Huxiao Sun, Dzmitry Shcharbin, Serge Mignani, Jean-Pierre Majoral, Mingwu Shen, Xiangyang Shi","doi":"10.1021/acsbiomaterials.4c02109","DOIUrl":"10.1021/acsbiomaterials.4c02109","url":null,"abstract":"<p><p>Tuberculosis (TB) is a severe respiratory infectious disease caused by <i>Mycobacterium tuberculosis</i> (<i>M.tb</i>), which puts enormous pressure on public health and economic systems worldwide. Therefore, accurate diagnosis and timely intervention of TB are critical for interrupting disease transmission and reducing mortality among TB patients. However, the low bioavailability, inadequate targeting, and significant adverse side effects of conventional antibiotics and the emergence of the multidrug-resistant <i>M.tb</i> strain result in limited TB treatment efficacy or even the development of multidrug-resistant TB. The development of nanomaterials provides new perspectives to improve the drawbacks of antibiotics for improved TB treatment, while enabling the diagnosis of TB. Herein, we review the conventional and nanotechnology-based diagnosis and intervention strategy of TB and the currently developed novel methods to solve the TB dilemma.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":" ","pages":"2492-2501"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprinted Hormone-Responsive Bilayer Model of Human Endometrium for Embryo Implantation Studies.","authors":"Liora Jacobs Catane, Erez Asher, Reuven Reich, Tali Tavor Re'em","doi":"10.1021/acsbiomaterials.4c02473","DOIUrl":"10.1021/acsbiomaterials.4c02473","url":null,"abstract":"<p><p>Implantation failure is a major challenge in reproductive medicine, with two-thirds of cases attributed to poor uterine receptivity. Current models have limited utility in capturing the complexities of the endometrium. This study introduces a novel bioprinted endometrial model with epithelial and stromal cells in a bilayer structure, designed to replicate the hormone-regulated endometrial environment and support embryo implantation studies. Alginate-based bioink formulations, cross-linked with calcium chloride or calcium gluconate, were optimized for 3D cell bioprinting, based on key parameters: reduced spreading ratio, lower printed line width standard deviation (SD), slower degradation rates, and enhanced cell viability. Human endometrial epithelial (RL95-2) and stromal (T HESCs) cell lines were encapsulated in the bioink and bioprinted in a bilayer structure: Clear stratification mimicking the layered architecture of native endometrium was confirmed using fluorescent microscopy. Sequential hormonal treatments with estradiol (proliferative phase), followed by estradiol and progesterone (secretory phase) highlighted the model's hormone-responsiveness. Estradiol significantly enhanced cell viability by day 2, while progesterone reduced cell viability by day 5, consistent with adaptation to the proliferative and secretory phases. Hormone-treated constructs displayed significantly lower E-cadherin expression, higher mRNA expression of various integrins and of vascular endothelial growth factor (VEGF), and reduced metalloproteinase (MMP)-2 secretion after 5 days, mirroring in vivo endometrial remodeling under progesterone influence. JAR spheroids, representing human blastocyst cells, adhered to and infiltrated the epithelial layer of the hormone-treated, bilayered model, effectively simulating embryo implantation. This bioprinted bilayer endometrial model represents a significant advancement in reproductive biology. It offers a platform for studying in vitro endometrial receptivity and implantation and paves the way for personalized treatment approaches in recurrent implantation failure.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2922-2934"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Quantitative Evaluation of the Efficacy of Endochondral Ossification-Based Grafts in Bone Defect Regeneration: An Analysis of Animal Studies.","authors":"Yihua Shi, Ranning Zhuo, Weichun Guo, Yubo Shi","doi":"10.1021/acsbiomaterials.4c01895","DOIUrl":"10.1021/acsbiomaterials.4c01895","url":null,"abstract":"<p><p>The regeneration of bone defects through bone grafts primarily depends on two strategies: intramembrane ossification (IO) and endochondral ossification (EO). Traditional bone tissue engineering has focused on mimicking the IO process to stimulate the formation of a bone-like matrix. However, repair strategies based on IO often result in excessive deposition of the matrix on the graft surface, hindering bone tissue regeneration. In recent years, researchers have increasingly focused on investigating the reparative potential of EO-based grafts for bone defects, such as microspheres, pellets, and hydrogel. However, the effectiveness of EO-based grafts on bone defects has not yet been quantitatively evaluated. Therefore, this study conducted a systematic review and meta-analysis of previous studies to quantitatively assess the bone regenerative potential of EO-based grafts. The results revealed that EO-based grafts showed favorable ability for bone regeneration. However, there was no significant difference in bone regeneration between EO-based grafts that utilized chondrogenic differentiation or hypertrophic differentiation. Additionally, the results demonstrated low quality in the experimental methods and the reporting of animal studies as well as a low quality of evidence provided by the included studies. Based on this, we propose three suggestions to enhance the quality of experimental methods and reporting in animal experiments. Furthermore, it is essential to conduct more evidence-based research to establish reliable evidence for the clinical application of EO-based grafts.</p>","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":"11 5","pages":"2481-2491"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}