Biochemistry and analytical biochemistry : current research最新文献

{"title":"Variation Effects of Mobile Phone Frequencies and Exposure Durations on Selected Oxidative Stress Biomarkers of Male Wistar Albino Rats","authors":"Iheme Ci, Omeh Yn, Nwuke Cp","doi":"10.4172/2161-1009.1000360","DOIUrl":"https://doi.org/10.4172/2161-1009.1000360","url":null,"abstract":"The effects of different frequencies and exposure duration on selected oxidative stress biomarkers of male albino wistar rats were studied. Sixty (60) rats were used for the study. The rats were divided into two major groups of thirty (30) rats each, and each major group were further divided into sub-groups, I-VI, of five (5) rats each according to their weights. The rats in major groups, A and B, were exposed for ninety (90) and one hundred and eighty (180) days respectively. The rats in sub-group I, II, and III were exposed to 900 MHz field from mobile phones for 5 h/day, 10 h/day, 15 h/day respectively, while the rats in subgroup IV were exposed to 2.4 GHz field for 24 h/day. Rats in subgroups V and VI were the normal and negative controls respectively. At the end of 90 and 180 days, the rats were anaesthetized; blood samples were collected for assessment of oxidative stress biomarkers. The activities of SOD, CAT and the concentration of MDA from subgroup III were 12.69 ± 0.60, 14.74 ± 0.07, and 2.03 ± 0.10 respectively. The activities of SOD, CAT and the concentration of MDA at these varying exposure time for 90 days duration were significantly (p<0.05) higher than those of positive and negative controls which were 8.64 ± 0.35, 9.60 ± 0.47, 1.03 ± 0.02 and 9.04 ± 0.02, 10.43 ± 0.51, 1.20 ± 0.20 respectively. At the exposure duration of 180 days, the activities of the SOD, CAT and the concentration of MDA were assessed. The activities of SOD, CAT and the concentration of MDA from subgroup III were 13.47 ± 0.51, 14.84 ± 0.07, and 2.11 ± 0.10 respectively. The activities of SOD, CAT and the concentration of MDA at these varying exposure time for 180 days were significantly (p<0.05) higher than those of positive and negative controls which were 8.91 ± 0.10, 9.94 ± 0.13, 1.18 ± 0.19; and 9.09 ± 0.03, 10.45 ± 0.49, 1.38 ± 0.20 respectively. At the different frequency of 2.4 GHz, the activities of the enzyme and the concentration of MDA were also assayed. At the exposure of 90 days duration, the activities of SOD, CAT and the concentration of MDA were 14.49 ± 0.29, 15.77 ± 0.10, and 2.67 ± 0.04 respectively. At the exposure of 180 days duration, the activities of SOD, CAT and MDA were 14.96 ± 0.10, 15.86 ± 0.11, and 3.00 ± 0.08 respectively. In general, the activities of SOD, CAT and MDA at the frequency of 2.4 GHz for the exposure duration of 90 and 180 days were significantly (p<0.05) higher than those positive and negative controls. From our findings, it can be concluded that different frequencies (900 MHz and 2.4 GHz) and exposure durations (90 and 180 days), can induce oxidative stress in living system. Variation Effects of Mobile Phone Frequencies and Exposure Durations on Selected Oxidative Stress Biomarkers of Male Wistar Albino Rats","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000360","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70555897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation and Destruction of Thermo-Lipofuscin in Mitochondria","authors":"Vekshin Nl, Frolova Ms","doi":"10.4172/2161-1009.1000357","DOIUrl":"https://doi.org/10.4172/2161-1009.1000357","url":null,"abstract":"","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70555890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Disorders Cardiac: Disturbances Triggers by Lack of ATP?","authors":"Pedro Ob","doi":"10.4172/2161-1009.1000358","DOIUrl":"https://doi.org/10.4172/2161-1009.1000358","url":null,"abstract":"Occidental lifestyle including lack of physical activity, are related to risk of developing heart diseases. ATP has a central role in understanding cardiac metabolism. Thus, this study was carried out to discuss aerobic cardiac metabolism, with most of the energy provided by oxidative phosphorylation. The ATP produced by this pathway is essentially used for contraction in non-pathological conditions. In addition, situations are discussed on the glycolytic pathway to produce ATP under pathological conditions. Thus, in some cardiomyopathies, the manipulation of the metabolic status of the heart represents an attractive alternative for the prevention of later complications. Metabolic Disorders Cardiac: Disturbances Triggers by Lack of ATP?","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-molecules for Kidney Stones Treatment and Management","authors":"G. S., Kanwar Ss","doi":"10.4172/2161-1009.1000362","DOIUrl":"https://doi.org/10.4172/2161-1009.1000362","url":null,"abstract":"It has been centuries since humans are being affected by kidney stones or urolithiasis. In the past decade, in rural and the urban sectors, kidney stone cases are rising at an alarming rate having a high relapse rate. The rate and prevalence of urolithiasis has been attributed to a number of factors such as age, fluid intake, infections of the urinary tract, climatic conditions sex, genetic predisposition, ethnicity as well as diet. Kidney stones may cause extreme pain and blockage of urine flow, urinary tract infection, hydronephrosis and severe bleeding which necessitates the use of surgery in some cases to remove or break the stones. Although a number of treatments for kidney stones are available which include extracorporeal shock wave lithotripsy (ESWL) and drug therapy but the expensive nature of these therapies and the severe side effects caused by exposure to these shock waves such as acute renal injury, decreased renal function and increased stone recurrence limit their use. As proposed by several in vivo and in vitro studies and clinical trials, using phyto-molecules in the treatment and management of kidney stones has emerged as a novel option. The following study discuses about the various plants, their chemical constituents involved along with their mechanism of action that can be used for the treatment of kidney stones.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutagens and Carcinogens Called Aflatoxins and Their Hydroxylated Metabolites in Food for Domestic Cats","authors":"Martinez-Ruiz Nc, M. Carvajal-Moreno, F. Rojo-Callejas, S. Fuentes-Daza, A. Gomez-Carrion, S. Ruiz-Velasco","doi":"10.4172/2161-1009.1000366","DOIUrl":"https://doi.org/10.4172/2161-1009.1000366","url":null,"abstract":"Aflatoxins (AFs) in cat food are a serious threat to cat health. The exposure of cats to AFs can cause damage depending on the exposure time and dosage, as well as the diet, nutritional state, age and sex of the cat. The first acute effect of AFs is liver damage, including cellular necrosis, hemorrhage, fibrosis, cirrhosis, immunosuppression, respiratory infection, anorexia, and fever. Chronic exposure to AFs can lead to hepatitis, cirrhosis and cancer in the liver, kidneys, lungs, colon and nervous system. Domestic cats can tolerate up to 0.55 mg of AFB1 per kg of body weight, which is the Lethal Dose 50% (LD50) that causes acute toxicity. Subacute aflatoxicosis (0.5-1 mg of AF/kg of food for cats) produces anorexia, drowsiness, jaundice, intravascular coagulation, hematomas, hemorrhagic gut and death in 2 to 3 weeks. For cats, hepatotoxic effects occur with chronic exposure to AFs at 0.05-0.3 mg of AF/kg of food over 6 to 8 weeks.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"29 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Ethanol Stem Bark Extract of Treculia africana on the Haematological Parameters of Albino Wistar Rats","authors":"Promise N, Godfrey Cd, Nwabu Ec","doi":"10.4172/2161-1009.1000347","DOIUrl":"https://doi.org/10.4172/2161-1009.1000347","url":null,"abstract":"This study evaluated the effect of ethanol stem bark extract of Treculia africana (African bread fruit) on haematological status in wistar rats. Twenty four wistar rats were divided into four (4) groups. Group I received daily oral dose of 5 mg/kg of the extract, Group II received daily oral dose of 15 mg/kg of the extract, Group Ill received daily oral dose of 25 mg/kg of the extract while Group IV received normal saline and served as the control. After 21 days of treatment, the rats were sacrificed and plasma obtained for haematological assay. Result showed that there was a significant (P 0.05) increase in test groups compared to control. There were significant (P<0.05) increases in mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) in group Ill (110.53 ± 0.15 and 50.83 ± 0.80) compared to control (100.76 ± 1.67 and 45.76 ± 0.82) respectively. Furthermore, the white blood cells (WBC) showed significant (P<0.05) increase in test groups (6.93 ± 0.50, 9.50 ± 0.57, 9.13 ± 0.42) compared to the control (5.23 ± 0.52). Lymphocytes (%) revealed a significant (P<0.05) decrease in group 1(36.56 ± 3.60) compared to control (4.66 ± 0.57) while neutrophils (%) showed a significant (P<0.05) increase in test groups (62.23 ± 2.51, 64.36 ± 3.78, 59.66 ± 4.50) compared to the control (50.20 ± 6.08). The results of this study revealed that the extract is capable of stimulating red blood cell formation (erythropoiesis) and an active phagocytic agent against foreign compounds and may be useful for haemopoietic conditions.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":" ","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47706913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on the Binding of Loperamide to Human Serum Albumin Using Combination of Computational and Experimental Methods","authors":"K. Sadrjavadi, F. Rahmati, Fataneh Jafari, S. Moradi, A. Nowroozi, M. Shahlaei","doi":"10.4172/2161-1009.1000346","DOIUrl":"https://doi.org/10.4172/2161-1009.1000346","url":null,"abstract":"In the present study, an attempt has been made to study the interaction of Loperamide, an drug used for treating symptoms or diarrhoea, with the most important transport protein, human serum albumin (HSA) employing UV-Vis, spectrofluorometric, FT-IR, Cyclic voltammetry, Electrochemical Impedance Spectroscopy and molecular modelling techniques. The results showed that binding of LOP to HSA caused strong fluorescence quenching of HSA through static quenching mechanism. The results of experimental and theoretical methods indicated that the binding of this drug to HSA induced slight conformational changes in HSA. Furthermore, the study of molecular modelling also showed that LOP could strongly bind to the site I (subdomain IIA) of HSA and hydrophobic interactions are the major forces in the stability of LOP-HSA complex.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"6 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43887041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Myeloma with Biclonal Gammopathy of IgA Kappa Variant: A Case Report","authors":"Anurag Yadav, R. Reddy, M. Malathi","doi":"10.4172/2161-1009.1000345","DOIUrl":"https://doi.org/10.4172/2161-1009.1000345","url":null,"abstract":"Introduction: Multiple myeloma is a plasma cell cancer causing infiltration of bone marrow and presence of monoclonal proteins in plasma and/or the Urine causing mortality and morbidity. Multiple myeloma with Biclonal Gammopathy is rare and accounting about 1-2%.Clinical case description: In this report, we encountered a patient presented with backache and weakness since a month, the lab investigation revealed reversal A/G ratio suspected towards para-protein and electrophoresis showed 2 distinct band in the gamma region, peripheral smear, bone marrow and X-ray reports supported findings, urine positive for Bence Jones Protein, Immunofixation showed 2 distinct bands in IgA and Kappa region. It is a rare Immunofixation pattern which is seen in any multiple myeloma cases. Conclusion: Hence with the clinical feature and the investigation the multiple myeloma was found to be positive, and 2 bands in IgA and kappa region pointing it to be a Biclonal Gammopathy.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"6 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000345","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49010524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidemic Properties of Sorghum vulgare Leaf Sheath on Oxidative Markers and Heart Function Enzymes of Dyslipidemic Wistar-Albino Rats","authors":"B. AkuruUdiomine, Okoko Tebekeme","doi":"10.4172/2161-1009.1000343","DOIUrl":"https://doi.org/10.4172/2161-1009.1000343","url":null,"abstract":"Dyslipidemia is an abnormal amount of lipids such as triglycerides, cholesterol and/or fat phospholipids in the blood. The aim of this study was to investigate the lipidemic properties of Sorghum vulgare leaf sheath on oxidative markers and heart function enzymes on high-fat-diet induced dyslipidemic wistar-albino rats. Thirty-six (36) wistar-albino rats weighing 110-130 g were used for the study. The animals were distributed randomly into six groups of six animals each. Group 1 (control), group II to group VI were fed with high fat diet; group II (untreated), groups III to V received 400 mg/kg, 800 mg/kg and 1200 mg/kg aqueous extract of Sorghum vulgare leaf sheath respectively while group VI was treated with atorvastatin (0.2 mg/kg) which is a standard drug. The results of the study showed that aqueous extract of Sorghum vulgare leaf sheath (especially at 800 mg/kg) significantly reduced (p ≤ 0.05) body-weight, triglyceride concentration, very low density lipoprotein concentration, creatinine concentration and Lactate dehydrogenase activity. Malondialdehyde concentration, cholesterol concentration, glutathione peroxidase activity, creatine kinase activity and superoxide dismutase activity do not differ significantly (p ≤ 0.05). The results suggest that Sorghum vulgare leaf sheath has myocardial protective properties.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"6 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000343","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47556460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ProTSAV+: A meta-server for identification and scoring of protein tertiary structures","authors":"A. Kuntal, B. Jayaram","doi":"10.4172/2161-1009-C1-014","DOIUrl":"https://doi.org/10.4172/2161-1009-C1-014","url":null,"abstract":"","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70562956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}