Biochemistry and analytical biochemistry : current research最新文献

{"title":"Screening of Trachyspermum ammi Antibacterial Activity","authors":"Khan Nt, N. Jameel","doi":"10.4172/2161-1009.1000359","DOIUrl":"https://doi.org/10.4172/2161-1009.1000359","url":null,"abstract":"Trachyspermum ammi’s seeds/leaves aqueous, ethanolic and methanolic extracts were screened for its antibacterial activity against Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Lactobacillus acidophilus, Micrococcus luteus, Staphylococcus aureus, Streptococcus pneumoniae and Pseudomonas aeruginosa. Among all the extracts, ethanolic extract of Trachyspermum ammi’s seeds were found to be effective against all the test bacteria but its aqueous extract was effective only against Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa. While it’s methanolic extract showed inhibitory potential against a number of test bacteria. In case of Trachyspermum ammi’s leave extracts, alcoholic extracts exhibited low antibacterial activity and the aqueous extract possess no antibacterial property at all. Screening of Trachyspermum ammi Antibacterial Activity","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000359","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70555821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Related Biochemical Tests and PTH in End Stage Renal Disease Patients in Iran","authors":"S. AbdolrahimPoorHeravi, M. Zarebavani, N. Einollahi, F. Safari, N. Dashti","doi":"10.4172/2161-1009.1000364","DOIUrl":"https://doi.org/10.4172/2161-1009.1000364","url":null,"abstract":"Introduction: End-stage renal disease (ESRD) patients usually have abnormalities in various laboratory tests. The aim of this study is evaluating of the rate of various laboratory tests in ESRD patient to reduce the risk of mortality and prevent them of other diseases.Methods: In this cross-sectional study a total of 300 patients who had ESRD and had been on hemodialysis treatment for at least 6 months were selected. The laboratory tests consisted of measurement of high density lipoprotein, triglyceride, cholesterol, fasting blood sugar, parathyroid hormone, alanine transferase, aspartate transferase, alkaline phosphatase, creatinine, blood urea nitrogen, calcium, phosphorous, sodium, potassium, and albumin.Results: The study population included 52.3% males and 47.7% females with average age of 41.5 ± 14.3 years. The high serum level of Chol (˃200 mg/dL) and TG (˃160 mg/dL) are 48.7%, 32.3%, respectively. About 98% were in high risk of range HDL (˂45 mg/dL). All of participants had over normal range of BUN and about 96% of them were over normal range of Cr. Rate of high serum level of Na, K, P, and Ca were 3.7%, 52%, 54.3%, and 14.6%, respectively.Discussion: Depend the result of this study, can understand that great group of patients with ESRD have high risk serum level of lipid indexes. Recent studies showed that there is a clear correlation between HDL cholesterol and cardiovascular risk in ESRD patients and there is correlation between level of triglyceride level and CKD progression. The important risk factors for involving in ESRD are hyperphosphatemia, hypercalcemia, dyslipidemia, and hypoalbuminemia which impact on CVD morbidity directly.Conclusion: In this study observed that most of patient with ESRD have abnormality in their lipid profiles factors, mineral electrolytes factors, and PTH. Regular laboratory checkup can control renal failure (especially ESRD patients) patients. Proper care of body, balanced diet intake, plenty water intake during the day can help them to control these diseases.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70555858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Chelidonium majus Water Extract onto Cancer Cells and Platelets","authors":"Deulenko Vn, M. Ry, Chernyshenko Vo, A. Rebriev, Lugovskoy Ev, L. Garmanchuk, Pavliuk Ov","doi":"10.4172/2161-1009.1000370","DOIUrl":"https://doi.org/10.4172/2161-1009.1000370","url":null,"abstract":"The plant C. majus (Celandine) is a known source of biologically active compounds that are used in traditional medicine for preventing or curing cancer diseases. The growing interest in this plant is due to discovery of a number of plant-derived cytostatics and anti-blastic drugs. The goal of our study was to discover the influence of a water extract from these plants on cancer cells and to characterize the obtained extract using different analytical methods. Water extract of leaves of C. majus were obtained and lyophilized on TelStar LyoQuest (Spain). Composition of the extract was characterized using HPLC Agilent 1100, SDS-PAGE electrophoresis and MLADI-TOF Mass-spectrometry. ADP-induced platelet aggregation in the presence of obtained mixture was studied by aggregometry using Solar2110 (Belorussia). Effects of the extracts on proliferation of cancer cells was estimated using MTT-test. Investigation of C. majus extract demonstrated presence of chelerythrine and precursors of berberine alkaloids in it. Addition of C. majus extract to platelet rich plasma induced moderate platelet activation (up to 10%). This aggregation was reversible. Further ADP-induced activation of platelets pre-incubated with the extract was diminished on 30%. The extract of C. majus leaves also was shown to possess anti-proliferative agent that was demonstrated on MCF-7 cancer cells. The surviving of MCF-7 coulter cells was decreased to 40% in the presence of extract. In conclusion, direct action of C. majus extract components on platelet aggregation and cancer cell proliferation was observed. Further fractionation for the determination of functionally active components of C. majus extract is promising for biotechnology and medicine.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Truth of Eating Different Fruits, Herbs, Honey etc. as Good Treatments for all Diabetes Types and for Hypertension in Different Societies","authors":"Al-Darraji Ahk, Abd-Al-Saheeb Ys","doi":"10.4172/2161-1009.1000369","DOIUrl":"https://doi.org/10.4172/2161-1009.1000369","url":null,"abstract":"","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Bio-Oil from Agriculture Waste","authors":"O. A. Mohamed","doi":"10.4172/2161-1009.1000348","DOIUrl":"https://doi.org/10.4172/2161-1009.1000348","url":null,"abstract":"Biomass energy which includes producing energy fuel from plant is considered a promising source of renewable energy. This research aims to develop a new method to liquefy two types of agriculture waste and compare between their produced bio-oils in terms of ultimate production conditions, compositions and applications.Firstly, One gram of two different types of agriculture wastes include Corn sticks (CS) and Palm leaves (PL) were liquefied by autoclaving at 2.5 atm pressure and 220°C temperature. The liquefaction process had been performed in the range of 10 to 80 ml ethanol/g treated solid, and retention time ranges between 10 to 120 min., the produced bio-oil were extracted and separated into three fractions: volatile, light and heavy.The results show that, the maximum amount (0.04 g/g) of volatile bio-oil can be produced from CS at 40 ml ethanol/g treated solid and 30 min retention time. However maximum amount of light bio-oil (g/g) was obtained from CS at 30 ml ethanol/1 g treated solid and 60 min retention time. For heavy bio-oil, maximum amount (0.25 g/g) was derived from CS when the ratio between ethanol (ml): treated solid (g) is 30:1 and the retention time is 60 min.Experimental data had been analysed using matlab software to get the modules which give the relations between bio-oil produced, ethanol to solid ratio and retention time in order to get the ultimate conditions of the process. GC-MS and FTIR analysis has been done to identify the bio-oils compositions. The results show that produced bio-oil from CS is highly contained carbon atom from C20-C38 in addition it contains high percentage of C6-C9 atoms. Therefore, the bio-oil from CS can be used as bio-fuel. However, the produced bio-oil from PL is mainly unsaturated acids which has carbon atom from C10-C18. Thus, it has pharmaceutical applications.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"7 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70555254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer Cell Growth - A Mini Review Part-2: Crabtree Effect, Pasteur Effect, Pyruvate Kinasetrient","authors":"C. Koyunoğlu","doi":"10.4172/2161-1009.1000372","DOIUrl":"https://doi.org/10.4172/2161-1009.1000372","url":null,"abstract":"The objective of this work is to address the mechanism how cancer cell growth. Current study has actually featured the metabolic versatility significance in both in vivo and cultured cells. As an example, development in the extreme subcutaneous area in mice environment or glucose deprivation, generates a careful impact for KRAS anomalies in cancer cells of colon. For circumstances, altered KRAS low glucose conditions rendered cells tolerant. Likewise, in culture, cancer cells can reorganize their metabolic process making up for the loss of either glutamine or glucose, commonly making use of a nutrient filling metabolite pools usually be provided by the other. In this part of mini review Crabtree Effect, Pasteur Effect, Pyruvate Kinasetrient terms summarized.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interdisciplinary Character of Biochemistry and Analytical Biochemistry","authors":"Butnariu M, Sarac I","doi":"10.4172/2161-1009.1000367","DOIUrl":"https://doi.org/10.4172/2161-1009.1000367","url":null,"abstract":"Biochemistry is a modern frontier science with an interdisciplinary character. Biochemistry has close links with organic chemistry, which provides support for knowing the structure and properties of biomolecules. Analytical chemistry, colloidal chemistry and chelates complexes provide biochemistry with methods of identifying, separating and characterizing biomolecules as well as kinetic and thermodynamic interpretation of biochemical reactions. Biochemical processes are carried out at cellular structures, so biochemistry interferes with molecular biology first and with morphology, which can also be approached from the aspect of structural element chemistry. Physiology raises a higher level of knowledge of the laws of life phenomena, and modern genetics has in fact become biochemical genetics, because it has created the basis for the codification of the hereditary message and has established relations between nucleic acids and protein biosynthesis as a premise of self-reproduction of living organisms.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical, Phytochemical and Antioxidant Composition of Equisetum debile Roxb","authors":"T. Rehman, M. Shad, H. Nawaz, H. Andaleeb, M. Aslam","doi":"10.4172/2161-1009.1000368","DOIUrl":"https://doi.org/10.4172/2161-1009.1000368","url":null,"abstract":"Biochemical, phytochemical and antioxidant composition of Equistem debile R. stem was determined. The E. debile extract showed the presence of reducing and non reducing sugars, total sugars, free amino acids, water soluble proteins and salt soluble protein. Phytochemical screening confirmed the presence of tannins, saponins, flavonoids, terpenoids, cardiac glycosides and total phenolic acid contents. Trolox equivalent total antioxidant activity of methanolic extract determined by DPPH radical scavenging assay was found to be lower than that determined by Phosphomolybdenum assay while the ascorbic acid equivalent total antioxidant activity determined by DPPH radical scavenging assay was found to be higher than that determined by Phosphomolybdenum assay. High value of ABTS+ radical scavenging activity and low values of DPPH and hydroxyl radical scavenging activity and reducing power have been observed in E. debile extract as compared to Trolox and ascorbic acid. The data provides useful information regarding the nutritional and medicinal importance of E. debile and suggest that this plant possesses good antioxidant activity.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000368","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Serum Electrolytes among Psoriasis Patients: A Prospective Case-Control Study from Telangana, South India","authors":"T. Sudhakar, Sabitha, G. Rajkumar, C. JhansiRani, P. Pratyusha, J. Bheem, K. Venkataramana","doi":"10.4172/2161-1009.1000365","DOIUrl":"https://doi.org/10.4172/2161-1009.1000365","url":null,"abstract":"Background: Psoriasis is a non-contagious autoimmune skin disorder. Patients present with symptoms of abnormal patches on the skin, joints and nails, which are dry and itchy. There are many factors responsible for this disease which initiates an inflammatory response leading to abnormal keratinization of the skin. Electrolytes play a significant role in maintaining the cell function and fluid balance both within and surrounding the cell. Any disturbance in the electrolyte balance could cause fluid loss and cell death/damage.Objective: None of the previous research studies have evaluated the role of electrolytes in the management and progression of psoriasis. The present study is aimed to assess the levels of electrolytes (sodium and potassium) among patients diagnosed with psoriasis.Methods: A prospective case-control study was conducted, which included 25 patients diagnosed with psoriasis and equal number of age and sex matched control group without psoriasis. The study was carried out at the Chalmeda Anandrao institute of medical sciences, a tertiary care teaching hospital located at Karimnagar, Telangana, India. Blood samples were collected from all the subjects included in the study and serum electrolyte (sodium and potassium) levels were measured using an indicator electrode of standard potential.Results: The mean levels of serum electrolytes (sodium and potassium) and the ratio of sodium to potassium among the psoriasis patients and the control subjects. The results demonstrated an increase of electrolyte levels of sodium (151.04 ± 3.79; p<0.0001), sodium to potassium ratio (44.999 ± 5.37; p<0.0001) and a decrease in the levels of potassium (3.352 ± 0.28; p<0.0001) as compared to the controls. Conclusion: There is an evidence of electrolyte imbalance among psoriasis patients. The levels of sodium and sodium to potassium ratio increased and the potassium levels were reduced. Further studies including a larger group of psoriasis patients and a regular follow-up of serum electrolytes may improve the understanding of the role of electrolytes in psoriasis disease and its progression.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"07 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70556567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperosmotic versus Hypoosmotic Stress in Plants","authors":"V. Hýsková, H. Ryšlavá","doi":"10.4172/2161-1009.1000E170","DOIUrl":"https://doi.org/10.4172/2161-1009.1000E170","url":null,"abstract":"The balance of water between the cell and its environment is crucial to all organisms, but especially for root plant cells that are exposed to extrinsic condition directly. High salt concentration in soil leads to loose of water from the plant cell (hyper-osmotic stress). Sufficient supply with water causes that the plant cell is turgid (i.e. increases its volume, the cell membrane experiences turgor pressure from the cell interior against the resistance of the cell wall), which is healthy state for most plants. However, upon repeated flooding (e.g. in a tropical rain-forest climate), the plants may experience a prolonged hypo-osmotic stress. Whereas the hyper-osmotic stress is one of the most studied abiotic stress factor, hypo-osmotic is at the edge of scientists´ interest, still considered as “physiological conditions”. However, both types of osmotic stress are characterized by involvement of stress sensing, oxidative burst, and signal transduction. In this study, sensors of osmotic stress (mechano-sensitive ion channels), osmolytes, and processes followed hypo- and hyper-osmotic stress are emphasized, although hypo-osmotic stress in comparison to hyper- have to be largely studied.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"7 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1009.1000E170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70560500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}