A Study on the Binding of Loperamide to Human Serum Albumin Using Combination of Computational and Experimental Methods

Abstract

In the present study, an attempt has been made to study the interaction of Loperamide, an drug used for treating symptoms or diarrhoea, with the most important transport protein, human serum albumin (HSA) employing UV-Vis, spectrofluorometric, FT-IR, Cyclic voltammetry, Electrochemical Impedance Spectroscopy and molecular modelling techniques. The results showed that binding of LOP to HSA caused strong fluorescence quenching of HSA through static quenching mechanism. The results of experimental and theoretical methods indicated that the binding of this drug to HSA induced slight conformational changes in HSA. Furthermore, the study of molecular modelling also showed that LOP could strongly bind to the site I (subdomain IIA) of HSA and hydrophobic interactions are the major forces in the stability of LOP-HSA complex.