Translational biomedicine最新文献_第7页

A New Strategy and System for the Ex-Vivo Testicle Perfusion and Cryopreservation 睾丸体外灌注冷冻保存新策略与新体系

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.1000108

Mohamed S. A. Mohamed

引用次数: 0

A Novel Natural Remedy for Mild and Chronic Constipation 一种治疗轻度和慢性便秘的新型自然疗法

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100124

R. Hamidpour, L. Rashan

引用次数: 0

The Novel Remedy Useful for Maintaining Male Fertility 有助于维持男性生育能力的新疗法

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100123

R. Hamidpour, L. Rashan

引用次数: 1

Evaluation of Alpha One Anti-Trypsin andHaptoglobin in Hypertensive Patients in Elele Elele高血压患者α - 1抗胰蛋白酶和粘连珠蛋白的评价

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100131

Nnatuanya In, Obeagu Ei, N. Cic, Ogarkova Oa, Stephen Ec, A. Onah

{"title":"Evaluation of Alpha One Anti-Trypsin andHaptoglobin in Hypertensive Patients in Elele","authors":"Nnatuanya In, Obeagu Ei, N. Cic, Ogarkova Oa, Stephen Ec, A. Onah","doi":"10.21767/2172-0479.100131","DOIUrl":"https://doi.org/10.21767/2172-0479.100131","url":null,"abstract":"Inflammation is associated with different cardiovascular diseases like in hypertension. This study to evaluate the level of alpha one anti-trypsin (AAT) and haptoglobin in hypertension was carried out on patients attending Madonna University Hospital (MUTH), Elele, Rivers State, Nigeria. The research was done on a total of one hundred (100) subjects. Fifty (50) of this population were hypertensive patients (Test) while fifty (50) were the control. Their age ranged from 45-65 years. Alpha one anti-trypsin and haptoglobin were measured by Mispa Auto-analyzer Nephelometric immunoassay method. The results were analyzed using statistical package for social science (SPSS) version 20. The results showed a significant increase (P<0.05) in haptoglobin in hypertensive subjects (120.9 ± 54.90 mg/dl) when compared with the control (47.7 ± 32.61 mg/dl). Haptoglobin was elevated in hypertension. Hence, level of haptoglobin could be used to predict cardiovascular risk in patients with certain disease conditions. The results also showed significant increase (P<0.05) in the AAT level of hypertensive subjects (293.7 ± 59.86 mg/dl) compared to the control (158.8 ± 52.14 mg/dl) respectively. Alpha one anti-trypsin plays a role as a biomarker in hypertension.","PeriodicalId":89642,"journal":{"name":"Translational biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21767/2172-0479.100131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68104476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Anti-Inflammatory Effects of The Chloroform Extract of Annona muricata Leaves on Phospholipase A2 andProstaglandin Synthase Activities 番荔枝叶氯仿提取物对磷脂酶A2和前列腺素合成酶活性的抗炎作用

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100137

Oyekachukwu Ar, Elijah Jp, Eshu Ov, Nwodo Ofc

{"title":"Anti-Inflammatory Effects of The Chloroform Extract of Annona muricata Leaves on Phospholipase A2 andProstaglandin Synthase Activities","authors":"Oyekachukwu Ar, Elijah Jp, Eshu Ov, Nwodo Ofc","doi":"10.21767/2172-0479.100137","DOIUrl":"https://doi.org/10.21767/2172-0479.100137","url":null,"abstract":"This study ascertained the mechanisms of the anti-inflammatory activity of the total lipid (chloroform) extract of Annona muricata leaves. The plant material was extracted with a mixture of chloroform and methanol (2:1) and partitioned with 0.2 volume water. The chloroform extract was investigated for its effect on the in vitro activities of phospholipase A2, prostaglandin synthase and membrane stabilization. The extract significantly (p < 0.05) inhibited phospholipase A2 activity in a concentration-related manner compared to the control, with a range of 0.2 - 0.6 mg/ml inhibiting the enzyme activity by 23.91 - 43.48%. Effect of the extract on prostaglandin synthase activity showed a significant (p < 0.05) inhibition of enzyme activity at the doses 0.1, 0.5 and 1.0 mg/ml compared to the control. The highest percentage inhibition (87.46%) attained at 0.5 mg/ml was comparable to that of 1.0 mg/ml indomethacin. At various concentrations (0.1-0.8 mg/ml), the chloroform extract also significantly (p < 0.05) inhibited heat and hypotonicityinduced haemolysis of human red blood cells (HRBCs) compared to the control. The highest percentage inhibition of heat-induced haemolysis (53.03%) was obtained at 0.4 mg/ml of the extract while the highest percentage inhibition of hypotonicity-induced haemolysis (77.91%) was obtained at 0.8 mg/ml. This study thus confirmed that the mode of action of this extract of Annona muricata leaves on inflammation could be through the inhibition of phospholipase A2 and prostaglandin synthase activities and by membrane stabilization.","PeriodicalId":89642,"journal":{"name":"Translational biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21767/2172-0479.100137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68105165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Importance of Screening for Mild Depression in Adults with Diabetes 糖尿病成人轻度抑郁筛查的重要性

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.1000101

K. Wilhelm, J. Reddy, J. Crawford, L. Robins, L. Campbell, J. Proudfoot

{"title":"The Importance of Screening for Mild Depression in Adults with Diabetes","authors":"K. Wilhelm, J. Reddy, J. Crawford, L. Robins, L. Campbell, J. Proudfoot","doi":"10.21767/2172-0479.1000101","DOIUrl":"https://doi.org/10.21767/2172-0479.1000101","url":null,"abstract":"Aim: While the impact of major depression on diabetes has gained increasing attention, the role of minor depression is less well investigated. This observational study compared three groups of adults with Type 1 (T1DM) or Type 2 (T2DM) diabetes mellitus: with me) no depressive symptoms (NODEP), ii) mild depressive symptoms (MILD) and iii) moderate-to-severe symptoms (MOD/SEVERE)-on diabetes distress, psychological distress, anxiety and somatic symptoms, quality of life and HbA1c levels. Methods: 245 outpatients attending two hospital diabetes services (27% with T1DM; 73% with T2DM) completed self-report measures including patient health questionnaire (PHQ), problem areas in diabetes (PAID) scale and short form health survey (SF12). Participants were interviewed by a psychiatrist and HbA1c levels recorded. Results: Half the sample reported depressive symptoms on the PHQ-9 (29% in the MILD group, 21% in MOD/ SEVERE and the remaining 50% in the NODEP group). Compared to the NODEP group, MILD group participants had significantly higher levels of diabetes distress, psychological distress, anxiety and somatic symptoms and significantly lower mental-health related quality of life. Participants in the MOD/SEVERE group had significantly higher levels of diabetes distress, psychological distress, anxiety, and somatic symptoms than either of the other two groups, and significantly lower quality of life. The groups did not differ significantly on HbA1c. Conclusions: Mild symptoms of depression in patients with diabetes are common and are associated with higher levels of diabetes distress, psychological distress, anxiety and somatic symptoms and lower quality of life. Addressing mild depression provides a potential avenue for preventing clinical depression in patients with diabetes.","PeriodicalId":89642,"journal":{"name":"Translational biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21767/2172-0479.1000101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68088147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A Natural Gastroprotective Remedy that Lowers Hyperacidity 一种天然的胃保护疗法，降低胃酸过多

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100129

R. Hamidpour, L. Rashan

引用次数: 0

Correlation of Urinary Foam with Proteinuria in Patients with Chronic Kidney Disease 慢性肾病患者尿泡与蛋白尿的相关性

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100110

J. Pisharam, R. Daiwajna, V. Chong, K. Mam, A. Liew, Ching Fe, Jackson Tan

{"title":"Correlation of Urinary Foam with Proteinuria in Patients with Chronic Kidney Disease","authors":"J. Pisharam, R. Daiwajna, V. Chong, K. Mam, A. Liew, Ching Fe, Jackson Tan","doi":"10.21767/2172-0479.100110","DOIUrl":"https://doi.org/10.21767/2172-0479.100110","url":null,"abstract":"Background: Foamy urine is often reported to be associated with proteinuria and kidney diseases. There is no objective measure on the nature and correlation of this relationship. Literature reviews revealed very few studies that can confirm or refute this relationship. This study hypothesized that the severity and persistence of urinary foam were associated with the degree of proteinuria (as determined by urine protein-creatinine ratio and dipstick). Methods: We analyzed urine samples of patients from our chronic kidney disease (CKD) clinics for urine protein and foam. The urine samples were shaken in a standardized way and heights of resting foam (in millimeters) were measured after a pre-determined resting time. The foam heights were correlated with clinical variables including proteinuria, stages of CKD, gender, age, co-morbidities and urine specific gravity. Results: A total of 160 urine samples were analyzed. Greater foam height was significantly associated with advanced CKD stages (p=0.015), urine dipstick protein (p<0.001), urine PCR (p=0.005) and diabetes mellitus (p=0.013). Urine specific gravity (p=0.053) and hypertension (p=0.91) did not achieve statistically significant results with foam height. Further analyses were performed on 54 urine samples with no or low protein level (defined as urine PCR of less than <100 mg/mmol AND urine protein dipstick result of ≤ 1+). In these samples, there was no statistically significant relationship between foam height and CKD stages (p=0.403), specific gravity (p=0.564), diabetes mellitus (p=0.909) and hypertension (p=0.08). Conclusion: Our study showed that urinary foam can be used as a rudimentary surrogate marker for proteinuria in patients with CKD. It provides extra leverage to the age-long assumptions that urinary foam is associated with kidney disease and proteinuria. More research will be needed to ascertain and investigate the nature of this relationship.","PeriodicalId":89642,"journal":{"name":"Translational biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21767/2172-0479.100110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68100720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Hypothetical Identification of Contra Distinct Human Lung Cancer Cell Lines as Natural Targets in Translational Research 在转化研究中，不同的人肺癌细胞系作为自然靶点的假设鉴定

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100112

W. Onuigbo

引用次数: 0

Characterization of Chromosomal Aberrationsin Neuroblastoma Formalin-Fixed Paraffin-Embedded Specimens with StandardArrayCGH Procedure - Preliminary Experience 神经母细胞瘤中染色体畸变的鉴定-用标准射线cgh程序进行福尔马林固定石蜡包埋标本-初步经验

Translational biomedicine Pub Date : 2017-01-01 DOI: 10.21767/2172-0479.100117

K. Szewczyk

{"title":"Characterization of Chromosomal Aberrationsin Neuroblastoma Formalin-Fixed Paraffin-Embedded Specimens with StandardArrayCGH Procedure - Preliminary Experience","authors":"K. Szewczyk","doi":"10.21767/2172-0479.100117","DOIUrl":"https://doi.org/10.21767/2172-0479.100117","url":null,"abstract":"Background: The broad spectrum of neuroblastoma (NB) clinical behavior depends on a genomic landscape of tumor cells. The amplification of MYCN oncogene is the most powerful negative prognostic marker in NB. Moreover, segmental chromosomal alterations are also associated with a poor outcome. Therefore, the comprehensive characterization of tumor genetic features is obligatory for NB patients. These features determine the risk stratification and therapeutic decisions in treatment. Purpose: Our report focuses on a possibility to use standard microarray procedure to demonstrate critical structural chromosomal alteration in archival samples of NB tumors. Methods and Findings: Formalin-fixed paraffin-embedded tissue samples from 8 NB primary tumors have been analyzed by cytogenetic microarrays. It achieved a very good quality of genomic DNA from fixed samples. Chromosomal abnormalities were detected in 7 out of 8 cases. It was not an incidence of MYCN amplification. Conclusion: The results demonstrate that it is possible to obtain reliable and highquality microarray data from archival samples.","PeriodicalId":89642,"journal":{"name":"Translational biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21767/2172-0479.100117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68102317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2