A New Strategy and System for the Ex-Vivo Testicle Perfusion and Cryopreservation

Childern, who suffer from cancer, receive gonadotoxic therapy, which destroys their future fertile abilities after survival. Testicular cryopreservation and transplantation provides the promising solution to this problem, where the testicle can be removed before the gonadotoxic therapy and reimplanted after patient's survival, where the testicle is to be cryopreserved during the period of the therapy. However, the effective cryopreservation of the whole testicle can be obstacle by the ischemic reperfusion injury and the defective cryopreservation related to the defective ability to universally deliver the cryopreservation/warming solutions through the organ vascular bed. To overcome those obstacles, an innovative system is suggested to insure immediate and universal delivery of the cryopreservation/warming solutions to the graft, in addition to keep the graft under continuous perfusion before and after cryopreservation, minimizing any chance for microthrombi formation or ischemia-reperfusion.