Biomedicines最新文献

{"title":"<i>CLIC4</i> Is a New Biomarker for Glioma Prognosis.","authors":"Zhichun Liu, Junhui Liu, Zhibiao Chen, Xiaonan Zhu, Rui Ding, Shulan Huang, Haitao Xu","doi":"10.3390/biomedicines12112579","DOIUrl":"10.3390/biomedicines12112579","url":null,"abstract":"<p><strong>Background: </strong>Chloride Intracellular Channel 4 (<i>CLIC4</i>) plays a versatile role in cellular functions beyond its role in primary chloride ion transport. Notably, many studies found an association between <i>CLIC4</i> expression and cancers. However, the correlation between <i>CLIC4</i> and glioma remains to be uncovered.</p><p><strong>Methods: </strong>A total of 3162 samples from nine public datasets were analyzed to reveal the relationship between <i>CLIC4</i> expression and glioma malignancy or prognosis. Immunohistochemistry (IHC) staining was performed to examine the results in an in-house cohort. A nomogram model was constructed to predict the prognosis. Functional enrichment analysis was employed to find <i>CLIC4</i>-associated differentially expressed genes in glioma. Immune infiltration analysis, correlation analysis, and IHC staining were employed, aiming to examine the correlation between <i>CLIC4</i> expression, immune cell infiltration, and ECM (extracellular matrix)-related genes.</p><p><strong>Results: </strong>The expression level of <i>CLIC4</i> was correlated with the malignancy of glioma and the prognosis of patients. More aggressive gliomas and mesenchymal GBM are associated with a high expression of <i>CLIC4</i>. Gliomas with IDH mutation or 1p19q codeletion express a low level of <i>CLIC4</i>, and a high expression of <i>CLIC4</i> correlates with poor prognosis. The nomogram model shows a good predictive performance. The DEGs (differentially expressed genes) in gliomas with high and low <i>CLIC4</i> expression are enriched in extracellular matrix and immune functions. On the one hand, gliomas with high <i>CLIC4</i> expression have a greater presence of macrophages, neutrophils, and eosinophils; on the other hand, a high <i>CLIC4</i> expression in gliomas is positively associated with ECM-related genes.</p><p><strong>Conclusions: </strong>Compared to glioma cells with low <i>CLIC4</i> expression, gliomas with high <i>CLIC4</i> expression exhibit greater malignancy and poorer prognosis. Our findings indicate that a high level of <i>CLIC4</i> correlates with high expression of ECM-related genes and the infiltration of macrophages, neutrophils, and eosinophils within glioma tissues.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiogenesis of Avascular Necrosis of the Femoral Head: A Classic Treatment Strategy.","authors":"Ping Wang, Wenkai Shao, Yuxi Wang, Bo Wang, Xiao Lv, Yong Feng","doi":"10.3390/biomedicines12112577","DOIUrl":"10.3390/biomedicines12112577","url":null,"abstract":"<p><p>Avascular necrosis of the femoral head (ANFH) is a type of osteonecrosis due to the cessation of blood supply, characterized by persistent local pain and collapse of the joint. The etiology of ANFH is multifaceted, and while its precise pathogenesis remains elusive, it is currently widely believed that the femoral head is highly dependent on the vascular system. A large number of studies have shown that vascular injury is the initial factor in the onset of ANFH. In this review, we briefly introduced the process of angiogenesis and the blood supply to the femoral head, with a focus on summarizing the existing research on promoting angiogenesis for the treatment of ANFH. We conclude that providing alternative pathways through angiogenesis to resolve the problem of the obstructed free flow of the blood is an important means of treating ANFH. Moreover, we also looked forward to the mechanism of endothelial metabolism, which has not yet been studied in femoral head necrosis models, providing potential strategies for more effective use of angiogenesis for the treatment of femoral head necrosis.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Chloride Cotransporter in Hypertension.","authors":"Annalisa Castagna, Gabriele Mango, Nicola Martinelli, Luigi Marzano, Sara Moruzzi, Simonetta Friso, Francesca Pizzolo","doi":"10.3390/biomedicines12112580","DOIUrl":"10.3390/biomedicines12112580","url":null,"abstract":"<p><p>The sodium chloride cotransporter (NCC) is essential for electrolyte balance, blood pressure regulation, and pathophysiology of hypertension as it mediates the reabsorption of ultrafiltered sodium in the renal distal convoluted tubule. Given its pivotal role in the maintenance of extracellular fluid volume, the NCC is regulated by a complex network of cellular pathways, which eventually results in either its phosphorylation, enhancing sodium and chloride ion absorption from urines, or dephosphorylation and ubiquitination, which conversely decrease NCC activity. Several factors could influence NCC function, including genetic alterations, hormonal stimuli, and pharmacological treatments. The NCC's central role is also highlighted by several abnormalities resulting from genetic mutations in its gene and consequently in its structure, leading to dysregulation of blood pressure control. In the last decade, among other improvements, the acquisition of knowledge on the NCC and other renal ion channels has been favored by studies on extracellular vesicles (EVs). Dietary sodium and potassium intake are also implicated in the tuning of NCC activity. In this narrative review, we present the main cornerstones and recent evidence related to NCC control, focusing on the context of blood pressure pathophysiology, and promising new therapeutical approaches.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Off-Label Use of Monoclonal Antibodies for Eosinophilic Esophagitis in Humans: A Scoping Review.","authors":"Benyu Yang, Wenhan Li, Yiqiang Gao, Bo Zhang, Wei Zuo","doi":"10.3390/biomedicines12112576","DOIUrl":"10.3390/biomedicines12112576","url":null,"abstract":"<p><p><b>Background</b>: Eosinophilic esophagitis (EoE) is a rare, chronic immune-mediated disorder with limited treatment options. Despite the U.S. Food and Drug Administration (FDA) approval of dupilumab for EoE, other monoclonal antibodies remain unapproved and are used off-label with limited evidence on their efficacy and safety. This systematic review rigorously and comprehensively evaluates the evidence for monoclonal antibody therapies used off-label to treat EoE. <b>Methods</b>: We conducted a systematic review across PubMed, EMBASE, Cochrane Central, and ClinicalTrials.gov, assessing the efficacy and safety of off-label monoclonal antibodies in EoE through clinical outcomes and the FDA Adverse Event Reporting System (FAERS) data. <b>Results</b>: Among ten monoclonal antibodies reviewed, mepolizumab that targets IL-5 showed the most promise with a moderate recommendation based on Level 2 evidence. Others like omalizumab (anti-IgE), dectrekumab (anti-IL-13), and reslizumab (anti-IL-5) showed limited utility. Safety evaluations via the FAERS database revealed significant adverse drug reactions, including serious events like asthmatic crises, pneumonia, and adrenal insufficiency for mepolizumab and reslizumab, as well as chronic obstructive pulmonary disease and gastroenteritis for omalizumab. Dectrekumab's safety profile remains unclear due to a lack of data. <b>Conclusions</b>: While mepolizumab demonstrates potential as an off-label treatment, none of the antibodies reviewed have FDA approval for EoE. Clinicians should consider the balance between local and systemic effects and exercise caution, closely monitoring for adverse effects, particularly in patients with respiratory comorbidities. Continued research is crucial to establish a more robust evidence base for these therapies.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Tolerability of a Shorter Agalsidase Beta Infusion Time in Patients with Classic or Later-Onset Fabry Disease.","authors":"Dominique P Germain, Alice Porto Vasconcelos, Lien Tran Thi Phuong, Najya Bedreddine, Mihaela Turcan, Wenting Trang, Lynda Barache","doi":"10.3390/biomedicines12112578","DOIUrl":"10.3390/biomedicines12112578","url":null,"abstract":"<p><strong>Background: </strong>The multisystem manifestations of Fabry disease can create major challenges in patient care. Although enzyme replacement therapy with recombinant agalsidase beta has demonstrated clinical benefits, the standard fortnightly, multi-hour infusion regimen imposes a substantial burden on patients.</p><p><strong>Methods: </strong>We assessed the safety and feasibility of shortening the agalsidase beta infusion time to 90 min in adult patients with classic or later-onset Fabry disease in the absence of premedication. A total of 39 consecutive adult patients (agalsidase-naïve: n = 7; with significant comorbidities: n = 15) with no recent infusion-associated reactions underwent a total of 85 agalsidase beta infusions in our tertiary reference centre for lysosomal diseases. Each infusion was administered at a constant rate (between 0.78 and 1.17 mg/min, depending on the total dose administered).</p><p><strong>Results: </strong>No adverse events of any type (including discomfort and infusion-associated reactions) were reported during or after infusions. The patients' vital signs and physical examination remained stable, and patients' satisfaction was high.</p><p><strong>Conclusions: </strong>Our results suggest that shortening the agalsidase beta infusion time to 90 min is safe and feasible in stably treated adult patients with Fabry disease and no recent infusion-associated reactions.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate-Poly[2-(methacryloyloxy)ethyl]trimethylammonium Chloride (PMETAC) Immunoisolating Capsules Prolong the Viability of Pancreatic Islets In Vivo.","authors":"Polina Ermakova, Ekaterina Vasilchikova, Arseniy Potapov, Maxim Baten'kin, Liya Lugovaya, Alexandra Bogomolova, Julia Tselousova, Alexey Konev, Natalia Anisimova, Alena Egoshina, Mariya Zakharina, Nasipbek Naraliev, Denis Kuchin, Vladimir Zagainov, Sergey Chesnokov, Aleksandra Kashina, Elena Zagaynova","doi":"10.3390/biomedicines12112573","DOIUrl":"10.3390/biomedicines12112573","url":null,"abstract":"<p><strong>Background/objectives: </strong>This study focuses on the development and evaluation of novel alginate-poly[2-(methacryloyloxy)ethyl]trimethylammonium chloride (PMETAC) microcapsules for encapsulating pancreatic islets to address insulin deficiency in diabetes.</p><p><strong>Methods: </strong>In previous research, we fabricated and characterized PMETAC microcapsules, evaluating their stability and permeability in vitro. This study further probes the capsules in vivo, focusing on the functional activity of the encapsulated islets post-transplantation, their viability extension, and the assessment of the immunoprotective, antifibrotic properties, and biostability of the capsules.</p><p><strong>Results: </strong>Rabbit-derived islets were encapsulated and transplanted into diabetic rats. The encapsulated islets maintained insulin secretion for up to 90 days, significantly longer than non-encapsulated ones, which ceased functioning after 7 days. Histological analysis demonstrated high biocompatibility of the PMETAC coating, resulting in minimal fibrotic overgrowth around the capsules.</p><p><strong>Conclusions: </strong>The study highlights the critical role of immunoprotection and the tendency to reduce fibrosis in prolonging islet function. These findings suggest that PMETAC-coated capsules offer a promising solution for cell-based therapies in diabetes by improving graft longevity and reducing fibrotic overgrowth.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurocognitive and Neuropsychiatric Implications of Fibrosing Interstitial Lung Diseases.","authors":"Zsolt Vastag, Emanuela Tudorache, Daniel Traila, Ovidiu Fira-Mladinescu, Monica Steluta Marc, Cristian Oancea, Elena Cecilia Rosca","doi":"10.3390/biomedicines12112572","DOIUrl":"10.3390/biomedicines12112572","url":null,"abstract":"<p><p>Patients with interstitial lung diseases (ILDs) associate a large variety of comorbidities that have a significant impact on their clinical outcomes and survival. Among these comorbidities is neurological impairment. This review highlights what is known about the cognitive function, central nervous system (CNS), depression, and anxiety in patients with specific forms of fibrosing ILDs, such as idiopathic pulmonary fibrosis, sarcoidosis, hypersensitivity pneumonitis, connective tissue diseases, etc. The most common pathogenic mechanisms for neurocognitive dysfunction as well as the screening methods and tools for their identification are also described in this review.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tbp</i> and <i>Hprt1</i> Are Appropriate Reference Genes for Splenic Neutrophils Isolated from Healthy or Tumor-Bearing Mice.","authors":"Khetam Sounbuli, Ludmila A Alekseeva, Aleksandra V Sen'kova, Innokenty A Savin, Marina A Zenkova, Nadezhda L Mironova","doi":"10.3390/biomedicines12112571","DOIUrl":"10.3390/biomedicines12112571","url":null,"abstract":"<p><p><b>Background</b>/<b>Objectives</b>: Neutrophils have recently gained significant attention due to their heterogeneity in tumor settings. The gene expression profiles of neutrophils from different tumor types are of great interest. Murine splenic neutrophils reflect the immune status of the organism and could be a source of tumor-associated neutrophils in tumor-bearing mice. However, information about appropriate reference genes for RT-qPCR analysis of murine neutrophils in the literature is lacking. The aim of this study was to identify stably expressed reference genes in murine splenic neutrophils. <b>Methods</b>: Bone marrow- and spleen-derived neutrophils were isolated from healthy C57Bl/6 and CBA/LacSto mice. Spleen-derived neutrophils were isolated from mice with Lewis lung carcinoma (LLC) and drug-resistant lymphosarcoma (RLS₄₀). RNA was isolated and used for RT-qPCR analysis of 10 selected reference genes. Analysis of reference gene stability was performed using four different algorithms (BestKeeper, NormFinder, geNorm, ΔCt method), and comprehensive ranking was constructed using RefFinder. <b>Results</b>: The Ct values for the reference genes were in the range of 16.73-30.83 with the highest expression levels observed for B2m and the lowest for Sdha. Differences in the stability ranking performed by different algorithms were observed; however, the overall ranking of the studied reference genes was as follows, from most to least stably expressed: <i>Tbp</i>, <i>Hprt1</i>, <i>Ywhaz</i>, <i>B2m</i>, <i>Gapdh</i>, <i>Actb</i>, <i>Sdha</i>, <i>Eef2</i>, <i>Rack1</i>, and <i>Rpl13a</i>. Using <i>Tbp</i> or <i>Rpl13a</i> for RT-qPCR data normalization significantly affected the interpretation of target gene expression. <b>Conclusions</b>: <i>Tbp</i> and <i>Hprt1</i> are recommended reference genes for murine splenic neutrophils regardless of their activation status.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iota-Carrageenan from Marine Alga <i>Solieria filiformis</i> Prevents Naproxen-Induced Gastrointestinal Injury via Its Antioxidant and Anti-Inflammatory Activities.","authors":"João L S Pinheiro, Willer M Sousa, Lucas H M Rodrigues, Francisco F Bezerra, Cecília L O A Cunha, Victória M R Santos, Samara R B D Oliveira, Rudy D Bingana, André Luiz R Barbosa, Marcellus H L P Souza, Ana Lúcia P Freitas, Renan O S Damasceno","doi":"10.3390/biomedicines12112574","DOIUrl":"10.3390/biomedicines12112574","url":null,"abstract":"<p><p><b>Background:</b> Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in therapy due to their anti-inflammatory and analgesic properties. However, their clinical use is often associated with gastrointestinal complications. Thus, this study aimed to investigate the protective effect of a sulfated iota-carrageenan isolated from the marine alga <i>Solieria filiformis</i> (IC-Sf) against naproxen-induced gastrointestinal injury. <b>Methods:</b> Parameters of gastrointestinal injury, secretory and motor functions, and toxicity were evaluated. <b>Results:</b> The results demonstrated that IC-Sf significantly reduced naproxen-induced gastrointestinal macroscopic injury, with a maximum effect observed at 30 mg/kg. IC-Sf also preserved gastrointestinal antioxidant defense and prevented lipid peroxidation, with a reduction in the non-protein sulfhydryl group (NP-SH) and malondialdehyde (MDA) concentrations induced by naproxen. Additionally, IC-Sf mitigated naproxen-induced gastrointestinal inflammation, as evidenced by reduced myeloperoxidase (MPO) activity, tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β). IC-Sf did not alter gastric secretion or gastrointestinal motility. In addition, the animals treated with IC-Sf did not present toxic effects. <b>Conclusions:</b> In conclusion, IC-Sf protected the gastrointestinal tract against the harmful effects of naproxen by inhibiting the inflammatory response and lipid peroxidation, suggesting its potential as a new therapeutic agent or food additive.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled Coffee Intake Enhances Erythrocyte Deformability, Na,K-ATPase Activity, and GSH/GSSG Ratio in Healthy Young Adults.","authors":"Dominika Radosinska, Tomas Jasenovec, Alzbeta Golianova, Ivan Szadvari, Rastislav Vazan, Ivona Kovacicova, Denisa Snurikova, Norbert Vrbjar, Jana Radosinska","doi":"10.3390/biomedicines12112570","DOIUrl":"10.3390/biomedicines12112570","url":null,"abstract":"<p><strong>Background: </strong>Published studies suggest that regular coffee consumption may reduce the risk of various diseases. However, many of these studies relied on questionnaire-based data, limiting their ability to identify the specific biological mechanisms behind the observed effects. This study focuses on controlled coffee consumption among healthy young adults to clarify its effects on erythrocyte properties. The functional condition of erythrocytes is important as it affects both macro- and microcirculation. Additionally, since erythrocytes are not true cells, they are particularly sensitive to biochemical and biophysical changes when exposed to biologically active substances.</p><p><strong>Methods: </strong>After a washout period, 33 healthy young volunteers were asked to consume a standardized dose of a coffee beverage daily for 3 weeks. Basic hematological and body composition parameters were recorded before and after the intervention. Erythrocyte functional status was evaluated based on the following measurements: deformability, osmotic resistance, Na,K-ATPase activity, and nitric oxide production, along with monitoring oxidative stress markers.</p><p><strong>Results: </strong>After a coffee consumption period, both erythrocyte count and hematocrit value increased, while body composition remained unchanged. Erythrocyte deformability improved across a range of shear stress values typical of human circulation. This improvement was accompanied with enhanced Na,K-ATPase activity in erythrocyte membranes in the wide range of sodium ion concentrations, as well as increased nitric oxide production by erythrocytes. Additionally, a higher GSH/GSSG ratio, indicating a shift towards a more favorable antioxidant balance, was observed in erythrocytes following the coffee intake period.</p><p><strong>Conclusions: </strong>The results of this study suggest that controlled coffee intake in healthy young adults can positively influence various indices of erythrocyte functional status. Although the observed statistically significant changes were modest, the findings consistently indicate a positive modulation of erythrocyte properties-cell deformability, oxidative resilience, and active membrane transport of cations-following coffee consumption.</p>","PeriodicalId":8937,"journal":{"name":"Biomedicines","volume":"12 11","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142725531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}