Biomarkers最新文献

{"title":"CD105-microvessel density analysis and its clinical value in urothelial carcinoma of bladder patients.","authors":"Rohit Siddhartha, Atin Singhai, Apul Goel, Minal Garg","doi":"10.1080/1354750X.2024.2435876","DOIUrl":"10.1080/1354750X.2024.2435876","url":null,"abstract":"<p><strong>Background: </strong>Endoglin/CD105-microvessel density (CD105-MVD) is identified as one of the most potential methods for semi-quantification of angiogenesis in human cancer tissues. Present study aimed to examine the diagnosticand prognostic value of CD105-MVD in two clinically distinct subtypes of urothelial carcinoma of bladder (UCB) namely non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) patients.</p><p><strong>Methods: </strong>Message expression of endoglin was analysed by real-time quantitative polymerase chain reaction (RT-qPCR) and MVD measurement was done by immunohistochemical staining in 90 UCB [NMIBC: 60; MIBC: 30] patients. SEM studies were carried out to examine tumor vasculature and extent of neoangiogenesis in NMIBC and MIBC patients.</p><p><strong>Results: </strong>Elevated message expression of CD105 showed statistical significance with tumor stage, grade, smoking/tobacco chewing history in NMIBC andage in MIBC cohort. Higher values of CD105-MVD showed statistical relevance with tumor stage, grade, size, smoking/tobacco chewing history in NMIBC cohort. Kaplan Meier test identified high CD105-MVD as strong predictor of poor RFS in NMIBC patients.</p><p><strong>Conclusions: </strong>Association of CD105 expression and MVD with the clinicohistopathological features as well as poor survival outcomes potentially identify it as a preferred marker of clinical significance in a given cohort of UCB patients.Clinical significanceStrong association of CD105 at message level with the demographics of UCB patients identifies it as a marker of diagnosis in a given cohort of patients.Survival analysis examined CD105-MVD as an independent strong predictor of poor recurrence free survival in NMIBC patients.Present study provides clear evidence of increased vascular density, vascular sprouts proliferation and new blood vessel formation with disease aggressiveness indicating CD105 as a preferred marker of neoangiogenesis in the given cohort of patients.The study describes CD105-MVD as a biomarker of diagnosis and prognosis with the sensitivity of 91.67% and 93.33% in a given cohort of NMIBC and MIBC patients.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"23-36"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in exposure to tobacco-related harmful and potentially harmful constituents among adults who switched completely from smoking cigarettes to use of the JUUL2 system for six days.","authors":"Nicholas I Goldenson, Saul Shiffman, Douglas Oliveri, Qiwei Liang, Ryan A Black","doi":"10.1080/1354750X.2024.2448493","DOIUrl":"10.1080/1354750X.2024.2448493","url":null,"abstract":"<p><strong>Introduction: </strong>Adults who switch from smoking cigarettes to use of electronic nicotine delivery systems (ENDS) may reduce their exposure to harmful and potentially harmful constituents (HPHCs). This study assessed changes in exposure to HPHCs, assessed <i>via</i> biomarkers of exposure (BOEs), among adults who switched to a new ENDS product.</p><p><strong>Methods: </strong>Adults who smoke cigarettes (<i>N</i> = 89) were randomized to: (1) switch completely to using JUUL2 Virginia Tobacco (<i>N</i> = 24) or Polar Menthol (<i>N</i> = 24); (2) continue smoking usual brand (UB) cigarettes (<i>N</i> = 21); or (3) abstain from all tobacco/nicotine products (<i>N</i> = 20) for 6 d. Changes in exposure to nicotine and 11 other HPHCs from Baseline to Day 6 were compared among study groups.</p><p><strong>Results: </strong>Changes in nicotine exposure did not significantly differ between JUUL2 and UB Cigarette groups (<i>p</i>s > 0.37). Among participants who switched completely to JUUL2 products, median percent reductions (Day 6-Baseline) in non-nicotine BOEs ranged from 65% to 94%, significantly greater than changes in the UB Cigarette group (<i>p</i>s < 0.001). None of the non-nicotine BOEs significantly differed between the JUUL2 groups and the Abstinence group (<i>p</i>s > 0.025).</p><p><strong>Conclusions: </strong>This randomized study demonstrates that adults who switch completely from smoking cigarettes to use of JUUL2 ENDS products substantially reduce their exposure to HPHCs associated with smoking-related diseases.</p><p><strong>International standard registered clinical trial number: </strong>ISRCTN27662176.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"77-87"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for <i>Staphylococcus aureus</i> colonization in a general emergency department patient cohort - results of an observational cohort study.","authors":"Dorothee Riedlinger, Fabian Holert, Petra Gastmeier, Axel Kola, Anna Slagman, Martin Möckel","doi":"10.1080/1354750X.2025.2451950","DOIUrl":"10.1080/1354750X.2025.2451950","url":null,"abstract":"<p><strong>Background: </strong>Testing for <i>Staphylococcus aureus</i> (SA) colonization in emergency department (ED) patients may guide prevention strategies against hospital acquired infections (HAIs). This study determined the prevalence of SA carriers in a general ED population, characterized the population, and identified predictors for SA colonization.</p><p><strong>Methods: </strong>A prospective monocentric observational cohort study in a tertiary care hospital collected nasopharyngeal swabs in 1000 adult patients. Polymerase chain reaction (PCR) testing for methicillin resistant and methicillin sensitive SA (MRSA/MSSA) was performed. Risk factor questionnaires and routine data from the clinical information system were captured. Descriptive statistics and binary logistic regression models were applied to report prevalence and outcomes and to identify predictors.</p><p><strong>Results: </strong>The prevalence for SA was 33.7% (<i>n</i> = 328; 95%-CI: 30.7-36.8): MSSA 30.9% (<i>n</i> = 301; 95%-CI: 28.0-34.0) and MRSA 2.8% (<i>n</i> = 27; 95%-CI: 1.8-4.0). Key predictors of SA colonization included having a catheter (OR 2.0, 95%-CI 1.0-4.0, <i>p</i> = 0.044) and requiring nursing care (OR 1.9, 95%-CI: 1.2-2.9, <i>p</i> = .007), even after adjusting for age and sex.</p><p><strong>Conclusion: </strong>Testing strategies for SA detection in ED need to focus on vulnerable populations with an elevated risk for HAIs and associated adverse outcomes. Individuals requiring nursing care could be a key target population for screening efforts.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"97-103"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral blood CD8 + CD28+ T cells as predictive biomarkers for treatment response in metastatic colorectal cancer.","authors":"Fei Fei, Peihua Lu, Jingyi Ni","doi":"10.1080/1354750X.2024.2435867","DOIUrl":"10.1080/1354750X.2024.2435867","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is a substantial global health burden, with treatment outcomes significantly influenced by the interaction between the immune system and the tumor microenvironment.</p><p><strong>Objective: </strong>This study aims to investigate the role of peripheral blood immune cell subpopulations, particularly CD8+ CD28+ T cells, in predicting treatment response in metastatic CRC patients receiving bevacizumab combined with chemotherapy.</p><p><strong>Methods: </strong>A cohort of 45 CRC patients was analyzed. Flow cytometry was utilized to assess immune cell subpopulations.</p><p><strong>Results: </strong>Higher CD8+ CD28+ T cell counts were associated with better treatment responses, including improved objective response rates. In a murine CRC model, the combination therapy significantly inhibited tumor growth and enhanced immune cell function.</p><p><strong>Conclusion: </strong>These findings highlight the importance of CD8+ CD28+ T cells as potential biomarkers for predicting treatment outcomes in CRC. They also suggest that bevacizumab, when combined with chemotherapy, can modulate immune function and improve clinical efficacy.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"30 1","pages":"10-22"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on identification of diagnostic biomarkers in serum for papillary thyroid cancer in different iodine nutrition regions.","authors":"Zhiyong Liu, Wei Zhang, Chenguang Wang, Xuebin Wang, Jie Luo, Yan He, Yashu Zhang, Shiqi Chen, Qi Zhou, Dianjun Sun, Lijun Fan","doi":"10.1080/1354750X.2024.2445258","DOIUrl":"10.1080/1354750X.2024.2445258","url":null,"abstract":"<p><strong>Background: </strong>At present, there is a lack of efficient biomarkers for the diagnosis of thyroid cancer, and the influence of natural factors such as high iodine exposure on the expression of biomarkers remains unclear.</p><p><strong>Methods: </strong>Serum samples from papillary thyroid cancer (PTC) and non-cancer controls matched 1:1 in different iodine nutritional regions were analyzed metabolomically using an ultra-high performance liquid chromatography-Orbitrap Exploris mass spectrometry (UHPLC-OE-MS) platform. Then the data were randomly divided into training and test sets in a 1:1 ratio according to the different iodine nutritional regions and different PTC status. In the training set, differential metabolites were selected by multivariate statistical analysis methods, and the prediction models were then built using Random forest (RF), Gradient boosting machine (GBM), and Support vector machine (SVM) models. At last, their diagnostic effects were examined in the test set.</p><p><strong>Results: </strong>PTCs were significantly separated from non-cancer samples, and a total of 37 differentially expressed metabolites were selected. The results of pathway analysis showed that the PTC-related differential metabolites were mainly involved in the sphingolipid metabolism and glycerophosphate metabolism. The prediction models constructed by the 6 screened potential biomarkers could all better identify PTCs in the test set. The metabolomic fingerprinting between PTC and non-cancer groups in different water iodine regions did not show significant disturbance. However, high iodine exposure would effect on the expression of six metabolites, reflecting in a significantly different diagnostic efficacy in different water iodine regions.</p><p><strong>Conclusion: </strong>Serum metabolites have potential value as biomarkers of PTC, and iodine status affects the expression and even diagnostic levels of certain serum metabolites.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"37-46"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of pentraxin 3 and cathepsin B levels in pregnancies complicated by preeclampsia.","authors":"Gülnur Tanrıverdi Kılıç, Nefise Nazlı Yenigül, Burcu Dinçgez, Elif Yüce Bilgin, Ünal Kaan Kılıç","doi":"10.1080/1354750X.2024.2421884","DOIUrl":"10.1080/1354750X.2024.2421884","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to compare the levels of cathepsin B and pentraxin 3 in maternal serum of pregnant women with preeclampsia in the second trimester, to ascertain the impact of these levels on maternal and fetal outcomes, and to present a comprehensive analysis of the combined effects of cathepsin B and pentraxin 3 levels.</p><p><strong>Methods: </strong>This prospective case-control study was conducted at Bursa Yuksek Ihtisas Training and Research Hospital between 1 January 2022 and 31 December 2022. The study included 78 pregnant women diagnosed with preeclampsia and 78 healthy pregnant women in the second trimester, between the ages of 18 and 45. Once a diagnosis of preeclampsia was established, maternal serum samples were obtained from the pregnant women prior to the initiation of any therapeutic intervention. Once all samples had been collected, the values for cathepsin B and pentraxin 3 were determined using the ELISA method.</p><p><strong>Results: </strong>The results demonstrated a statistically significant elevation in the levels of pentraxin 3 (<i>p</i> = 0.008) and cathepsin B (<i>p</i> = 0.005) in pregnancies affected by preeclampsia when compared to those deemed healthy. Moreover, pentraxin-3 (<i>p</i> = 0.007) and cathepsin B (<i>p</i> = 0.002) were found to be significantly elevated in severe preeclampsia compared to mild preeclampsia. A comparison of the groups with and without HELLP syndrome revealed no statistically significant difference between the two groups. The ROC analysis revealed that the Cathepsin B 7.04 cut-off value was statistically significantly associated with the prediction of preeclampsia in all cases, with a sensitivity of 78.2% and a specificity of 47.4% (<i>p</i> = 0.005, AUC = 0.631).</p><p><strong>Conclusion: </strong>The levels of CB and PTX3 may be employed as biomarkers to facilitate the early diagnosis of PE during the second trimester. Furthermore, these biomarkers may prove to be promising for the prediction of PE severity.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"518-527"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a cell cycle-specific lncRNA-miRNA-mRNA network reveals novel key lncRNAs in colorectal cancer.","authors":"Marzieh Naderi Boldaji, Shahrzad Shahbazi, Somayeh Reiisi, Kambiz Ahmadi, Mohammad Mahdevar","doi":"10.1080/1354750X.2024.2431015","DOIUrl":"10.1080/1354750X.2024.2431015","url":null,"abstract":"<p><strong>Objective: </strong>The current study aimed to determine the roles of pivotal and novel lncRNAs associated with the cell cycle in the occurrence and development of Colorectal cancer (CRC).</p><p><strong>Methods: </strong>The TCGA-COAD project related to CRC was downloaded, and differential expression analysis was performed to identify differentially expressed lncRNAs, miRNAs, and mRNAs. A cell cycle-associated lncRNA-miRNA-mRNA regulatory network was constructed, and two novel lncRNAs were selected. Two subnetworks were constructed for selected lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were illustrated for the genes in each sub-network. qPCR analysis was used to validate the expression levels of the selected lncRNAs in CRC tissues compared to those adjacent normal tissues.</p><p><strong>Results: </strong>The differential expression analysis identified 416 lncRNAs, 317 miRNAs, and 117 mRNAs. The ceRNA subnetwork genes were associated with different pathways, including cellular senescence, DNA replication, human T-cell leukemia virus 1 infection, and oocyte meiosis. The bioinformatic results based on the TCGA project indicated the dysregulation of two novel lncRNAs, MIR29B2CHG and HELLPAR, in CRC tissues compared to adjacent normal tissues. Moreover, qPCR confirmed the dysregulation of lncRNAs in the CRC tissues. ROC curves revealed that both selected lncRNAs had acceptable specificity and sensitivity as biomarkers.</p><p><strong>Conclusion: </strong>In conclusion, novel cell cycle-associated lncRNAs have the potential to be understood as the underlying molecular mechanisms that influence CRC. Therefore, these lncRNAs can be considered as promising biomarkers for the diagnosis and treatment of CRC.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"565-576"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A data mining approach to identify key radioresponsive genes in mouse model of radiation-induced intestinal injury.","authors":"Suchitra Sharma, Aliza Rehan, Ajaswrata Dutta","doi":"10.1080/1354750X.2024.2420196","DOIUrl":"10.1080/1354750X.2024.2420196","url":null,"abstract":"<p><strong>Background: </strong>Radiation-mediated GI injury (RIGI) is observed in humans either due to accidental or intentional exposures. This can only be managed with supporting care and no approved countermeasures are available till now. Early detection and monitoring of RIGI is important for effective medical management and improve survival chances of exposed individuals.</p><p><strong>Objective: </strong>The present study aims to identify new signatures of RIGI using data mining approach followed by validation of selected hub genes in mice.</p><p><strong>Methods: </strong>Data mining study was performed using microarray datasets from Gene Expression Omnibus database. The differentially expressed genes were identified and further validated in total-body irradiated mice.</p><p><strong>Results: </strong>Based on KEGG pathway analysis, lipid metabolism was found as one of the predominant pathways altered in irradiated intestine. Extensive alteration in lipid profile and lipid modification was observed in this tissue. A protein-protein interaction network revealed top 08 hub genes related to lipid metabolism, namely Fabp1, Fabp2, Fabp6, Npc1l1, Ppar-α, Abcg8, Hnf-4α, and Insig1. qRT-PCR analysis revealed significant up-regulation of Fabp6 and Hnf-4α and down-regulation of Fabp1, Fabp2 and Insig1 transcripts in irradiated intestine. Radiation dose and time kinetics study revealed that the selected 05 genes were altered differentially in response to radiation in intestine.</p><p><strong>Conclusion: </strong>Finding suggests that lipid metabolism is one of the key targets of radiation and its mediators may act as biomarkers in detection and progression of RIGI.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"505-517"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of Talin-1 and HER-2 status in different types of gastric carcinoma.","authors":"Farideh Hashemi, Fatemeh Tajik, Leili Saeednejad Zanjani, Masoumeh Dehghan Manshadi, Sadegh Safaei, Pegah Babaheidarian, Fahimeh Fattahi, Roya Ghods, Zahra Madjd","doi":"10.1080/1354750X.2024.2423270","DOIUrl":"10.1080/1354750X.2024.2423270","url":null,"abstract":"<p><strong>Background: </strong>Talin-1 (TLN1) is crucial in cell migration, metastasis, and cancer development. This study evaluated Talin-1 expression and its clinical significance in gastric cancer (GC), along with human epidermal growth factor receptor-2 (HER-2) expression and its correlation with Talin-1.</p><p><strong>Methods: </strong>Bioinformatics analysis assessed the potential prognostic value of Talin-1 and HER-2 in GC patients. The study included 223 GC patients (Signet Ring Cells and Intestinal subtypes) and 29 non-malignant tissue samples. Immunohistochemistry (IHC) on tissue microarray slides evaluated Talin-1 and HER-2 expression and clinical significance. Receiver operating characteristic (ROC) curves assessed their diagnostic value.</p><p><strong>Results: </strong>Bioinformatics identified Talin-1 as a potential prognostic factor and HER-2 as an oncogene in GC. Talin-1 and HER-2 expression increased in SRC-type GC samples compared to non-malignant tissues. High cytoplasmic Talin-1 expression inversely correlated with tumor expansion and invasion in SRC-type GC. Increased HER-2 expression positively correlated with metastasis. ROC curves showed significant diagnostic values for both proteins.</p><p><strong>Conclusions: </strong>Higher cytoplasmic Talin-1 expression is associated with less invasive tumor behavior, while increased membranous HER-2 expression is associated with metastasis in SRC-type GC. These findings suggest potential use in assessing diagnosis and screening high-risk cancer patients, particularly those with SRC-type GC.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"539-556"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severity prediction markers in dengue: a prospective cohort study using machine learning approach.","authors":"Aashika Raagavi Jean Pierre, Siva Ranganathan Green, Lokeshmaran Anandaraj, Manikandan Sivaprakasam, Anand Kasirajan, Panneer Devaraju, Srilekha Anumulapuri, Srinivasa Rao Mutheneni, Agieshkumar Balakrishna Pillai","doi":"10.1080/1354750X.2024.2430997","DOIUrl":"10.1080/1354750X.2024.2430997","url":null,"abstract":"<p><strong>Background: </strong>Dengue virus causes illnesses with or without warning indicators for severe complications. There are no clear prognostic signs linked to the disease outcomes.</p><p><strong>Methods: </strong>Clinical and laboratory parameters among 102 adult including 17 severe dengue (SD), 33 with warning and 52 without warning signs during early and critical phases were analysed by statistical and machine learning (ML) models.</p><p><strong>Results: </strong>In classical statistics, abnormal ultrasound findings, platelet count and low lymphocytes were significantly linked with SD during the febrile phase, while low creatinine, high sodium and elevated AST/ALT during the critical phase. ML models highlighted AST/ALT and lymphocytes as key markers for distinguishing SD from non-severe dengue, aiding clinical decisions.</p><p><strong>Conclusion: </strong>Parameters like liver enzymes, platelet counts and USG findings were linked with SD.USG testing at an earlier phase of dengue and a point-of-care system for the quantification of AST/ALT levels may lead to an early prediction of SD.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"557-564"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}