Biomarkers最新文献

{"title":"Peripheral blood CD8 + CD28+ T cells as predictive biomarkers for treatment response in metastatic colorectal cancer.","authors":"Fei Fei, Peihua Lu, Jingyi Ni","doi":"10.1080/1354750X.2024.2435867","DOIUrl":"10.1080/1354750X.2024.2435867","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is a substantial global health burden, with treatment outcomes significantly influenced by the interaction between the immune system and the tumor microenvironment.</p><p><strong>Objective: </strong>This study aims to investigate the role of peripheral blood immune cell subpopulations, particularly CD8+ CD28+ T cells, in predicting treatment response in metastatic CRC patients receiving bevacizumab combined with chemotherapy.</p><p><strong>Methods: </strong>A cohort of 45 CRC patients was analyzed. Flow cytometry was utilized to assess immune cell subpopulations.</p><p><strong>Results: </strong>Higher CD8+ CD28+ T cell counts were associated with better treatment responses, including improved objective response rates. In a murine CRC model, the combination therapy significantly inhibited tumor growth and enhanced immune cell function.</p><p><strong>Conclusion: </strong>These findings highlight the importance of CD8+ CD28+ T cells as potential biomarkers for predicting treatment outcomes in CRC. They also suggest that bevacizumab, when combined with chemotherapy, can modulate immune function and improve clinical efficacy.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"30 1","pages":"10-22"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on identification of diagnostic biomarkers in serum for papillary thyroid cancer in different iodine nutrition regions.","authors":"Zhiyong Liu, Wei Zhang, Chenguang Wang, Xuebin Wang, Jie Luo, Yan He, Yashu Zhang, Shiqi Chen, Qi Zhou, Dianjun Sun, Lijun Fan","doi":"10.1080/1354750X.2024.2445258","DOIUrl":"10.1080/1354750X.2024.2445258","url":null,"abstract":"<p><strong>Background: </strong>At present, there is a lack of efficient biomarkers for the diagnosis of thyroid cancer, and the influence of natural factors such as high iodine exposure on the expression of biomarkers remains unclear.</p><p><strong>Methods: </strong>Serum samples from papillary thyroid cancer (PTC) and non-cancer controls matched 1:1 in different iodine nutritional regions were analyzed metabolomically using an ultra-high performance liquid chromatography-Orbitrap Exploris mass spectrometry (UHPLC-OE-MS) platform. Then the data were randomly divided into training and test sets in a 1:1 ratio according to the different iodine nutritional regions and different PTC status. In the training set, differential metabolites were selected by multivariate statistical analysis methods, and the prediction models were then built using Random forest (RF), Gradient boosting machine (GBM), and Support vector machine (SVM) models. At last, their diagnostic effects were examined in the test set.</p><p><strong>Results: </strong>PTCs were significantly separated from non-cancer samples, and a total of 37 differentially expressed metabolites were selected. The results of pathway analysis showed that the PTC-related differential metabolites were mainly involved in the sphingolipid metabolism and glycerophosphate metabolism. The prediction models constructed by the 6 screened potential biomarkers could all better identify PTCs in the test set. The metabolomic fingerprinting between PTC and non-cancer groups in different water iodine regions did not show significant disturbance. However, high iodine exposure would effect on the expression of six metabolites, reflecting in a significantly different diagnostic efficacy in different water iodine regions.</p><p><strong>Conclusion: </strong>Serum metabolites have potential value as biomarkers of PTC, and iodine status affects the expression and even diagnostic levels of certain serum metabolites.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"37-46"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of pentraxin 3 and cathepsin B levels in pregnancies complicated by preeclampsia.","authors":"Gülnur Tanrıverdi Kılıç, Nefise Nazlı Yenigül, Burcu Dinçgez, Elif Yüce Bilgin, Ünal Kaan Kılıç","doi":"10.1080/1354750X.2024.2421884","DOIUrl":"10.1080/1354750X.2024.2421884","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to compare the levels of cathepsin B and pentraxin 3 in maternal serum of pregnant women with preeclampsia in the second trimester, to ascertain the impact of these levels on maternal and fetal outcomes, and to present a comprehensive analysis of the combined effects of cathepsin B and pentraxin 3 levels.</p><p><strong>Methods: </strong>This prospective case-control study was conducted at Bursa Yuksek Ihtisas Training and Research Hospital between 1 January 2022 and 31 December 2022. The study included 78 pregnant women diagnosed with preeclampsia and 78 healthy pregnant women in the second trimester, between the ages of 18 and 45. Once a diagnosis of preeclampsia was established, maternal serum samples were obtained from the pregnant women prior to the initiation of any therapeutic intervention. Once all samples had been collected, the values for cathepsin B and pentraxin 3 were determined using the ELISA method.</p><p><strong>Results: </strong>The results demonstrated a statistically significant elevation in the levels of pentraxin 3 (<i>p</i> = 0.008) and cathepsin B (<i>p</i> = 0.005) in pregnancies affected by preeclampsia when compared to those deemed healthy. Moreover, pentraxin-3 (<i>p</i> = 0.007) and cathepsin B (<i>p</i> = 0.002) were found to be significantly elevated in severe preeclampsia compared to mild preeclampsia. A comparison of the groups with and without HELLP syndrome revealed no statistically significant difference between the two groups. The ROC analysis revealed that the Cathepsin B 7.04 cut-off value was statistically significantly associated with the prediction of preeclampsia in all cases, with a sensitivity of 78.2% and a specificity of 47.4% (<i>p</i> = 0.005, AUC = 0.631).</p><p><strong>Conclusion: </strong>The levels of CB and PTX3 may be employed as biomarkers to facilitate the early diagnosis of PE during the second trimester. Furthermore, these biomarkers may prove to be promising for the prediction of PE severity.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"518-527"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a cell cycle-specific lncRNA-miRNA-mRNA network reveals novel key lncRNAs in colorectal cancer.","authors":"Marzieh Naderi Boldaji, Shahrzad Shahbazi, Somayeh Reiisi, Kambiz Ahmadi, Mohammad Mahdevar","doi":"10.1080/1354750X.2024.2431015","DOIUrl":"10.1080/1354750X.2024.2431015","url":null,"abstract":"<p><strong>Objective: </strong>The current study aimed to determine the roles of pivotal and novel lncRNAs associated with the cell cycle in the occurrence and development of Colorectal cancer (CRC).</p><p><strong>Methods: </strong>The TCGA-COAD project related to CRC was downloaded, and differential expression analysis was performed to identify differentially expressed lncRNAs, miRNAs, and mRNAs. A cell cycle-associated lncRNA-miRNA-mRNA regulatory network was constructed, and two novel lncRNAs were selected. Two subnetworks were constructed for selected lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were illustrated for the genes in each sub-network. qPCR analysis was used to validate the expression levels of the selected lncRNAs in CRC tissues compared to those adjacent normal tissues.</p><p><strong>Results: </strong>The differential expression analysis identified 416 lncRNAs, 317 miRNAs, and 117 mRNAs. The ceRNA subnetwork genes were associated with different pathways, including cellular senescence, DNA replication, human T-cell leukemia virus 1 infection, and oocyte meiosis. The bioinformatic results based on the TCGA project indicated the dysregulation of two novel lncRNAs, MIR29B2CHG and HELLPAR, in CRC tissues compared to adjacent normal tissues. Moreover, qPCR confirmed the dysregulation of lncRNAs in the CRC tissues. ROC curves revealed that both selected lncRNAs had acceptable specificity and sensitivity as biomarkers.</p><p><strong>Conclusion: </strong>In conclusion, novel cell cycle-associated lncRNAs have the potential to be understood as the underlying molecular mechanisms that influence CRC. Therefore, these lncRNAs can be considered as promising biomarkers for the diagnosis and treatment of CRC.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"565-576"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A data mining approach to identify key radioresponsive genes in mouse model of radiation-induced intestinal injury.","authors":"Suchitra Sharma, Aliza Rehan, Ajaswrata Dutta","doi":"10.1080/1354750X.2024.2420196","DOIUrl":"10.1080/1354750X.2024.2420196","url":null,"abstract":"<p><strong>Background: </strong>Radiation-mediated GI injury (RIGI) is observed in humans either due to accidental or intentional exposures. This can only be managed with supporting care and no approved countermeasures are available till now. Early detection and monitoring of RIGI is important for effective medical management and improve survival chances of exposed individuals.</p><p><strong>Objective: </strong>The present study aims to identify new signatures of RIGI using data mining approach followed by validation of selected hub genes in mice.</p><p><strong>Methods: </strong>Data mining study was performed using microarray datasets from Gene Expression Omnibus database. The differentially expressed genes were identified and further validated in total-body irradiated mice.</p><p><strong>Results: </strong>Based on KEGG pathway analysis, lipid metabolism was found as one of the predominant pathways altered in irradiated intestine. Extensive alteration in lipid profile and lipid modification was observed in this tissue. A protein-protein interaction network revealed top 08 hub genes related to lipid metabolism, namely Fabp1, Fabp2, Fabp6, Npc1l1, Ppar-α, Abcg8, Hnf-4α, and Insig1. qRT-PCR analysis revealed significant up-regulation of Fabp6 and Hnf-4α and down-regulation of Fabp1, Fabp2 and Insig1 transcripts in irradiated intestine. Radiation dose and time kinetics study revealed that the selected 05 genes were altered differentially in response to radiation in intestine.</p><p><strong>Conclusion: </strong>Finding suggests that lipid metabolism is one of the key targets of radiation and its mediators may act as biomarkers in detection and progression of RIGI.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"505-517"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of Talin-1 and HER-2 status in different types of gastric carcinoma.","authors":"Farideh Hashemi, Fatemeh Tajik, Leili Saeednejad Zanjani, Masoumeh Dehghan Manshadi, Sadegh Safaei, Pegah Babaheidarian, Fahimeh Fattahi, Roya Ghods, Zahra Madjd","doi":"10.1080/1354750X.2024.2423270","DOIUrl":"10.1080/1354750X.2024.2423270","url":null,"abstract":"<p><strong>Background: </strong>Talin-1 (TLN1) is crucial in cell migration, metastasis, and cancer development. This study evaluated Talin-1 expression and its clinical significance in gastric cancer (GC), along with human epidermal growth factor receptor-2 (HER-2) expression and its correlation with Talin-1.</p><p><strong>Methods: </strong>Bioinformatics analysis assessed the potential prognostic value of Talin-1 and HER-2 in GC patients. The study included 223 GC patients (Signet Ring Cells and Intestinal subtypes) and 29 non-malignant tissue samples. Immunohistochemistry (IHC) on tissue microarray slides evaluated Talin-1 and HER-2 expression and clinical significance. Receiver operating characteristic (ROC) curves assessed their diagnostic value.</p><p><strong>Results: </strong>Bioinformatics identified Talin-1 as a potential prognostic factor and HER-2 as an oncogene in GC. Talin-1 and HER-2 expression increased in SRC-type GC samples compared to non-malignant tissues. High cytoplasmic Talin-1 expression inversely correlated with tumor expansion and invasion in SRC-type GC. Increased HER-2 expression positively correlated with metastasis. ROC curves showed significant diagnostic values for both proteins.</p><p><strong>Conclusions: </strong>Higher cytoplasmic Talin-1 expression is associated with less invasive tumor behavior, while increased membranous HER-2 expression is associated with metastasis in SRC-type GC. These findings suggest potential use in assessing diagnosis and screening high-risk cancer patients, particularly those with SRC-type GC.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"539-556"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severity prediction markers in dengue: a prospective cohort study using machine learning approach.","authors":"Aashika Raagavi Jean Pierre, Siva Ranganathan Green, Lokeshmaran Anandaraj, Manikandan Sivaprakasam, Anand Kasirajan, Panneer Devaraju, Srilekha Anumulapuri, Srinivasa Rao Mutheneni, Agieshkumar Balakrishna Pillai","doi":"10.1080/1354750X.2024.2430997","DOIUrl":"10.1080/1354750X.2024.2430997","url":null,"abstract":"<p><strong>Background: </strong>Dengue virus causes illnesses with or without warning indicators for severe complications. There are no clear prognostic signs linked to the disease outcomes.</p><p><strong>Methods: </strong>Clinical and laboratory parameters among 102 adult including 17 severe dengue (SD), 33 with warning and 52 without warning signs during early and critical phases were analysed by statistical and machine learning (ML) models.</p><p><strong>Results: </strong>In classical statistics, abnormal ultrasound findings, platelet count and low lymphocytes were significantly linked with SD during the febrile phase, while low creatinine, high sodium and elevated AST/ALT during the critical phase. ML models highlighted AST/ALT and lymphocytes as key markers for distinguishing SD from non-severe dengue, aiding clinical decisions.</p><p><strong>Conclusion: </strong>Parameters like liver enzymes, platelet counts and USG findings were linked with SD.USG testing at an earlier phase of dengue and a point-of-care system for the quantification of AST/ALT levels may lead to an early prediction of SD.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"557-564"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics as a targeted intervention in anti-ageing: a review.","authors":"Fengcui Shi, Jingwen Peng, Haojin Li, Denghai Liu, Li Han, Ying Wang, Qingli Liu, Qian Liu","doi":"10.1080/1354750X.2024.2424388","DOIUrl":"10.1080/1354750X.2024.2424388","url":null,"abstract":"<p><strong>Context: </strong>The age-induced disruption of gut flora, termed gut dysbiosis, is intimately tied to compromised immune function, augmented oxidative stress and a spectrum of age-linked disorders.</p><p><strong>Objective: </strong>This review examines the fundamental mechanisms employed by probiotic strains to modulate gut microbiota composition and metabolic profiles, mitigate cognitive decline <i>via</i> the gut-brain axis (GBA), modulate gene transcription and alleviate inflammatory responses and oxidative stress.</p><p><strong>Conclusion: </strong>We elucidate the capacity of probiotics as a precision intervention to restore gut microbiome homeostasis and alleviate age-related conditions, thereby offering a theoretical framework for probiotics to decelerate ageing, manage age-related diseases, and elevate quality of life.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"577-585"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of CLDN18.2 in gastric cancer prognosis: a systematic review and meta-analysis.","authors":"Francisco Cezar Aquino de Moraes, Luis Eduardo Rodrigues Sobreira, Maria Eduarda Cavalcanti Souza, Rommel Mario Rodríguez Burbano","doi":"10.1080/1354750X.2024.2422965","DOIUrl":"10.1080/1354750X.2024.2422965","url":null,"abstract":"<p><strong>Background: </strong>Gastric cancer (GC) is a major global cause of cancer mortality, with a median overall survival of just 12 months. CLDN18.2, a specific isoform of Claudin18 normally expressed in the gastric mucosa, has emerged as a potential therapeutic target and prognostic biomarker due to its exposure on the surface of tumor cells following malignant transformation. This exposure allows CLDN18.2's extracellular loops to bind monoclonal antibodies, presenting new opportunities for targeted therapy and improved prognostic assessment.</p><p><strong>Methods: </strong>A comprehensive search of PubMed, EMBASE, Cochrane Library, and Web of Science databases was conducted for studies that addressed the correlation of CLDN18.2 with: (1) Progression-free survival (PFS) and (2) Overall Survival (OS). Hazard ratio (HR) and odds ratio (OR) with 95% confidence intervals (CIs) were calculated using a fixed-effects model. Heterogeneity was examined with <i>I</i>² statistics. <i>P</i> values of ≤ 0.05 were considered statistically significant. Statistical analyses were performed using RStudio, version 4.2.3.</p><p><strong>Results: </strong>A total of 15 studies encompassing a total of 4,085 patients were included. There were 2,691 (65.8%) male and 1,394 (34.2%) female patients. In the histologic GC analysis, there were 1,582 (38.7%) patients that had intestinal type and 1,280 (31.3%) with diffuse type. Patients with CLDN18.2 negative status exhibited a non-significant trend towards prolonged PFS (HR: 1.25; 95% CI: 0.98-1.61; <i>p</i> = 0.07; <i>I</i>² = 18%) and a significant prolonged OS (HR: 1.20; 95% CI: 1.07-1.34; <i>p</i> < 0.01; <i>I</i>² = 37%) when compared to CLDN18.2-positive patients.</p><p><strong>Conclusion: </strong>Our findings establish CLDN18.2 as a robust negative prognostic indicator for overall survival in GC patients. While its impact on PFS was not statistically significant, the association with OS suggests CLDN18.2 may serve as a marker for complex biological processes underlying tumor advancement.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"528-538"},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usability of presepsin, calprotectin, and interleukin 6 in the diagnosis of acute appendicitis.","authors":"Emine Ozdal, Fatma Tortum, Esra Laloglu, Esra Egilmez, Kamber Kasali","doi":"10.1080/1354750X.2024.2415077","DOIUrl":"10.1080/1354750X.2024.2415077","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate presepsin, calprotectin, and interleukin-6 levels together in terms of their utility in the diagnosis of acute appendicitis and distinguishing complicated acute appendicitis cases. In addition, it was attempted to identify a biomarker that would be most useful in diagnosing acute appendicitis.</p><p><strong>Methods: </strong>This study was conducted prospectively at the emergency department of a tertiary hospital. Patients diagnosed with AA from July 3, 2023, through January 1, 2024, were evaluated. 45 patients with acute appendicitis and 45 healthy volunteers were included in the study. Presepsin, calprotectin, and interleukin-6 levels were measured in both groups and subjected to statistical analyses.</p><p><strong>Results: </strong>To ensure equality between the two groups participating in the study, 17 female and 28 male patients were included in each group. The presepsin, calprotectin, and interleukin-6 levels of the patients with appendicitis were significantly higher than those of the healthy group (<i>p</i> < 0.001 for all). However, presepsin, calprotectin, and interleukin-6 were not significant parameters in differentiating between complicated and uncomplicated appendicitis (<i>p</i> = 0.493, <i>p</i> = 0.202, and <i>p</i> = 0.448, respectively).</p><p><strong>Conclusion: </strong>Presepsin, calprotectin, and interleukin-6 levels may be useful in diagnosing acute appendicitis but would be insufficient in identifying complicated cases.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"479-484"},"PeriodicalIF":4.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}