Biomarkers最新文献

{"title":"The role of exosomal hsa-miR-125b-5p and hsa-miR-320c as non-invasive biomarkers in high-radon areas of Kazakhstan.","authors":"Akmaral Aripova, Assiya Kussainova, Milana Ibragimova, Olga Bulgakova, Rakhmetkazhi Bersimbaev","doi":"10.1080/1354750X.2025.2456007","DOIUrl":"10.1080/1354750X.2025.2456007","url":null,"abstract":"<p><strong>Background: </strong>Radon, a radioactive gas, is a significant risk factor for lung cancer, especially in non-smokers. This study examines the expression of exosomal microRNAs (miRNAs) as potential biomarkers for radon-induced effects.</p><p><strong>Methods: </strong>A total of 109 participants from high- and low-radon areas in Kazakhstan were included. Exosomal hsa-miR-125b-5p and hsa-miR-320c levels were quantified using real-time PCR.</p><p><strong>Results: </strong>Results revealed a 25.4-fold increase in hsa-miR-125b-5p and a 12.5-fold decrease in hsa-miR-320c in participants exposed to high-radon levels compared to controls. Bioinformatic analysis identified key target genes, such as PRDM1 and IRF4, which are implicated in cancer development.</p><p><strong>Conclusion: </strong>These findings suggest that exosomal miRNAs could serve as non-invasive biomarkers for radon exposure, offering potential for early diagnosis and monitoring of radon-induced lung cancer. The study underscores the need for further research to validate these miRNAs as reliable diagnostic tools.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"123-130"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of LncRNAs G2E3-AS1 and BACE1-AS as prognostic biomarkers in metastatic colorectal cancer.","authors":"Shahrbanoo Nandoust Kenari, Parisa Mohamadynejad, Mehdi Moghanibashi, Abouzar Bagheri, Leila Rouhi","doi":"10.1080/1354750X.2024.2448508","DOIUrl":"10.1080/1354750X.2024.2448508","url":null,"abstract":"<p><strong>Background: </strong>Despite the current diagnostic and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer. Recently, numerous investigations have highlighted the role of lncRNAs in cancer development and progression. This study investigated less well-characterized genes in the colorectal cancer metastasis process.</p><p><strong>Materials and methods: </strong>Genes expression profiles from CRC patients were downloaded from the TCGA database by the TCGAbiolinks R package. Differential gene expression analysis of miRNA, lncRNAs, and mRNAs was conducted for the M1 and M0 compared to control samples. Then, the DIANA lncbase3 tool was used to find M1-specific miRNA-LncRNA interactions. In addition, the expression of selected genes was evaluated by Real-time RT-PCR in forty-one CRC tissues.</p><p><strong>Results: </strong>Our analysis showed that the expression levels of 77 lncRNAs, 12 miRNAs, and 627 mRNA were significantly changed only in metastatic tumors. In experimental study, significant overexpression of LncRNAs LINC00839, LINC01006, BACE1-AS and G2E3-AS1 was confirmed in metastatic tumors. Also, ROC analysis showed that these lncRNAs, especially lncRNAs G2E3-AS1 and BACE1-AS, are good prognostic biomarkers for metastatic colorectal tumors.</p><p><strong>Conclusion: </strong>We demonstrated that the lncRNAs G2E3-AS1 and BACE1-AS expression upregulated in CRC tissues can be good potential biomarkers for metastatic colorectal cancer.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"88-96"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutational and co-mutational landscape of early onset colorectal cancer.","authors":"Jumanah Yousef Alshenaifi, Guglielmo Vetere, Giulia Maddalena, Mahmoud Yousef, Michael G White, John Paul Shen, Eduardo Vilar, Christine Parseghian, Arvind Dasari, Van Karlyle Morris, Ryan Huey, Michael J Overman, Robert Wolff, Kanwal P Raghav, Jason Willis, Kristin Alfaro, Andy Futreal, Y Nancy You, Scott Kopetz","doi":"10.1080/1354750X.2024.2447089","DOIUrl":"10.1080/1354750X.2024.2447089","url":null,"abstract":"<p><strong>Introduction: </strong>Colorectal cancer (CRC) incidence and mortality before 50 have been rising alarmingly in the recent decades.</p><p><strong>Methods: </strong>Using a cohort of 10,000 patients, this study investigates the clinical, mutational, and co-mutational features of CRC in early-onset (EOCRC, < 50 years) compared to late-onset (LOCRC, ≥ 50 years).</p><p><strong>Results: </strong>EOCRC was associated with a higher prevalence of Asian and Hispanic patients, rectal or left-sided tumors (72% vs. 59%), and advanced-stage disease. Molecular analyses revealed differences in mutation patterns, with EOCRC having higher frequencies of <i>TP53</i> (74% vs. 68%, <i>p</i> < 0.01) and <i>SMAD4</i> (17% vs. 14%, <i>p</i> = 0.015), while <i>BRAF</i> (5% vs. 11%, <i>p</i> < 0.001) and <i>NOTCH1</i> (2.7% vs. 4.1%, <i>p</i> = 0.01) mutations were more prevalent in LOCRC. Stratification by tumor site and MSI status highlighted significant location- and age-specific molecular differences, such as increased <i>KRAS</i> and <i>CTNNB1</i> mutations in right-sided EOCRC and higher <i>BRAF</i> prevalence in MSI-H LOCRC (47% vs. 6.7%, <i>p</i> < 0.001). Additionally, co-occurrence analysis revealed unique mutational networks in EOCRC MSS, including significant co-occurrences of <i>FBXW7</i> with <i>NOTCH3</i>, <i>RB1</i>, and <i>PIK3R1</i>.</p><p><strong>Conclusion: </strong>This study highlights the significance of age-specific molecular profiling, offering insights into the unique biology of EOCRC and potential clinical applications.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"64-76"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and clinical significance of miR-421 in prostate cancer.","authors":"Xiaojuan Huang, Guifang He, Lulu Zheng, Yongping Cai, Yu Yin","doi":"10.1080/1354750X.2024.2445804","DOIUrl":"10.1080/1354750X.2024.2445804","url":null,"abstract":"<p><strong>Objective: </strong>To examine the role and diagnostic potential of miR-421 in prostate cancer (PCa).</p><p><strong>Methods: </strong>Expression data and clinical information for miR-421 were obtained from the TCGA and Genotype-Tissue Expression (GTEx) databases. Experimental validation was performed at the cellular, blood, and tissue levels to confirm miR-421 expression and its association with clinicopathological features. ROC curves were drawn on the bioinformatic study using TCGA data. The target genes of miR-421 were predicted via four online databases, and protein interaction associations were analyzed for intersecting targets. Gene Ontology (GO) analysis was subsequently conducted to assess functional relevance.</p><p><strong>Results: </strong>MiR-421 was significantly overexpressed in prostate cancer (PCa) patients, a finding validated in cell, blood, and tissue samples. ROC analysis on the bioinformatic study using TCGA data revealed that miR-421 reliably differentiated PCa tissues from normal tissues. Higher miR-421 expression was associated with an elevated Gleason score, advanced TNM stage, and metastasis. GO enrichment analysis indicated that the target genes of miR-421 were significantly related to diverse molecular functions.</p><p><strong>Conclusions: </strong>MiR-421 is a promising biomarker for diagnosing and predicting PCa.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"55-63"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of urine metabolomics in the diagnosis and management of adult and pediatric Crohn's disease and ulcerative colitis.","authors":"Kanish Baskaran, Michal Moshkovich, Lara Hart, Nyah Shah, Fariha Chowdhury, Meera Shanmuganathan, Philip Britz-McKibbin, Nikhil Pai","doi":"10.1080/1354750X.2024.2438734","DOIUrl":"10.1080/1354750X.2024.2438734","url":null,"abstract":"<p><strong>Introduction: </strong>Urine metabolomics offers a non-invasive approach to diagnose and manage inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), by identifying distinct metabolic signatures.</p><p><strong>Objectives: </strong>This narrative review summarizes current findings on urinary metabolites in IBD, evaluating their roles in disease differentiation, assessment of activity, and monitoring therapeutic response.</p><p><strong>Methods: </strong>A comprehensive literature search of PubMed and MEDLINE up to October 2023 was conducted using keywords, such as 'urine metabolomics', 'inflammatory bowel disease', 'Crohn's disease', 'ulcerative colitis', and 'urinary biomarkers'. Studies were included that described alterations to metabolic pathways, including those related to the urea cycle, central energy metabolism (Krebs cycle), amino acid metabolism, and neurotransmitters.</p><p><strong>Results: </strong>Specific urinary metabolites differentiate IBD patients from healthy controls and between CD and UC. Decreased urinary levels of hippurate, acetate, methanol, formate, and methylamine are observed in IBD, indicating altered gut microbiota. In CD patients, urea cycle alterations include reduced urinary urea and ornithine with increased arginine. Changes in Krebs cycle intermediates show decreased citrate and succinate in adults, but increased fumarate and isocitrate in pediatric patients, reflecting energy metabolism differences. Amino acid metabolism differs by age: Adults exhibit decreased urinary asparagine, lysine, and histidine, while pediatric patients show increased methionine, proline, aspartic acid, and isoleucine. Elevated urinary neurotransmitters like dopamine are noted in pediatric IBD patients. Urine metabolomics also can monitor treatment efficacy by distinguishing responders from non-responders to therapies and differentiating active disease from remission.</p><p><strong>Conclusion: </strong>Urine metabolomics provides promising, non-invasive biomarkers to enhance IBD diagnostics by distinguishing CD from UC and offering insights into underlying metabolic disturbances, paving the way for more precise, accessible patient care.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"104-113"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of long-term all-cause mortality after carotid artery stenting: evaluation of the Naples prognostic score.","authors":"Cemalettin Yılmaz, Muhammet M Tiryaki, Ahmet Karaduman, Büşra Güvendi Şengör, Tuba Unkun, Enise N Özlem Tiryaki, Hüseyin Akçalı, Barkın Kültürsay, Lütfi Öcal, Regayip Zehir","doi":"10.1080/1354750X.2024.2445801","DOIUrl":"10.1080/1354750X.2024.2445801","url":null,"abstract":"<p><strong>Background: </strong>Mortality in patients after carotid artery stenting (CAS), a treatment approach for atherosclerotic carotid artery stenosis, is influenced by numerous factors. This study aimed to investigate the prognostic value of the Naples prognostic score (NPS), which reflects nutritional and inflammatory status, in CAS patients.</p><p><strong>Methods: </strong>We retrospectively included 697 patients who underwent CAS from January 2016 to December 2020 at our institute. The primary endpoint of the study was long-term all-cause mortality. The study population was divided into two groups based on the NPS value: Low NPS (NPS 0-2) and high NPS (NPS 3-4). Univariable and multivariable Cox regression analysis was used to identify independent predictors of death.</p><p><strong>Results: </strong>The median follow-up time was 60.8 (46.36-75.36) months. During the follow-up period, all-cause mortality was higher in the high-NPS group compared to the low-NPS group [54% (n = 88) vs. 24% (n = 128) p < 0.001]. Advanced age (p = 0.003), diabetes (p = 0.023), and NPS (hazard ratio: 1.83, confidence interval: 1.58-2.12, p < 0.001) were found to be independent predictors of all-cause mortality at long-term follow-up.</p><p><strong>Conclusion: </strong>Consequently, NPS as a marker of malnutrition and inflammation, was found to be associated with long-term mortality and serves as an independent predictor of long-term mortality in patients undergoing CAS.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"47-54"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic value of neurohormonal and inflammatory biomarkers in addition to the TIMI risk score in patients with ST-elevation myocardial infarction.","authors":"Sarah Louise Duus Holle, Helle Søholm, Jacob Eifer Møller, Matilde Winther-Jensen, Joakim Bo Kunkel, Lisette Okkels Jensen, Jesper Kjærgaard, Matias Greve Lindholm, Ole Kristian Lerche Helgestad, Sebastian Wiberg, Rikke Reinholdt Sousa, Lene Holmvang, Jakob Hartvig Thomsen, Jens Peter Goetze, Christian Hassager, Martin Frydland","doi":"10.1080/1354750X.2024.2435866","DOIUrl":"10.1080/1354750X.2024.2435866","url":null,"abstract":"<p><strong>Background: </strong>The Thrombolysis in Myocardial Infarction (TIMI) risk score estimates mortality for patients with ST-elevation myocardial infarction (STEMI). This study aimed to investigate whether biomarkers reflecting the neurohormonal response (pro-atrial natriuretic peptide (proANP), mid-regional pro-adrenomedullin (MR-proADM), and copeptin), inflammation (suppression of tumorigenicity 2 (ST2), C-reactive protein (CRP), and leukocytes), and troponin add prognostic value to the TIMI risk score.</p><p><strong>Methods: </strong>This sub-study of the prospective PREDICT cohort included 1700 non-comatose and non-cardiogenic shock STEMI patients upon admission. Blood samples were collected before coronary angiography. Biomarker quartiles (Q4vsQ1-3) association with 30-day mortality were examined using Cox proportional hazard models.</p><p><strong>Results: </strong>High levels of all biomarkers were associated with 30-day mortality independently of TIMI risk score, hazard ratio (HR)_Q4vsQ1-3 (95%CI), MR-proADM: 8.8 (3.9-20), proANP: 3.5 (1.8-6.7), copeptin: 1.9 (1.1-3.5), ST2: 4.5 (2.3-8.6), CRP: 2.6 (1.3-4.9), and leukocyte: 2.18 (1.2;4.0). TIMI risk score had a high prognostic value, AUC(95%CI): 0.76 (0.69-0.83). Only MR-proADM, proANP, CRP, ST2, and TnT added prognostic value to the risk score, 0.84 (0.77-0.91), 0.80 (0.74-0.87), 0.78 (0.71-0.86), 0.81 (0.73-0.88), and 0.79 (0.71-0.87), respectively. However, MR-proADM demonstrated a higher prognostic value on its own (0.86 (0.80-0.91)).</p><p><strong>Conclusion: </strong>TIMI risk score and all the biomarkers added prognostic values of 30-day mortality. The strongest predictor of 30-day mortality was observed for MR-proADM alone.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD105-microvessel density analysis and its clinical value in urothelial carcinoma of bladder patients.","authors":"Rohit Siddhartha, Atin Singhai, Apul Goel, Minal Garg","doi":"10.1080/1354750X.2024.2435876","DOIUrl":"10.1080/1354750X.2024.2435876","url":null,"abstract":"<p><strong>Background: </strong>Endoglin/CD105-microvessel density (CD105-MVD) is identified as one of the most potential methods for semi-quantification of angiogenesis in human cancer tissues. Present study aimed to examine the diagnosticand prognostic value of CD105-MVD in two clinically distinct subtypes of urothelial carcinoma of bladder (UCB) namely non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) patients.</p><p><strong>Methods: </strong>Message expression of endoglin was analysed by real-time quantitative polymerase chain reaction (RT-qPCR) and MVD measurement was done by immunohistochemical staining in 90 UCB [NMIBC: 60; MIBC: 30] patients. SEM studies were carried out to examine tumor vasculature and extent of neoangiogenesis in NMIBC and MIBC patients.</p><p><strong>Results: </strong>Elevated message expression of CD105 showed statistical significance with tumor stage, grade, smoking/tobacco chewing history in NMIBC andage in MIBC cohort. Higher values of CD105-MVD showed statistical relevance with tumor stage, grade, size, smoking/tobacco chewing history in NMIBC cohort. Kaplan Meier test identified high CD105-MVD as strong predictor of poor RFS in NMIBC patients.</p><p><strong>Conclusions: </strong>Association of CD105 expression and MVD with the clinicohistopathological features as well as poor survival outcomes potentially identify it as a preferred marker of clinical significance in a given cohort of UCB patients.Clinical significanceStrong association of CD105 at message level with the demographics of UCB patients identifies it as a marker of diagnosis in a given cohort of patients.Survival analysis examined CD105-MVD as an independent strong predictor of poor recurrence free survival in NMIBC patients.Present study provides clear evidence of increased vascular density, vascular sprouts proliferation and new blood vessel formation with disease aggressiveness indicating CD105 as a preferred marker of neoangiogenesis in the given cohort of patients.The study describes CD105-MVD as a biomarker of diagnosis and prognosis with the sensitivity of 91.67% and 93.33% in a given cohort of NMIBC and MIBC patients.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"23-36"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in exposure to tobacco-related harmful and potentially harmful constituents among adults who switched completely from smoking cigarettes to use of the JUUL2 system for six days.","authors":"Nicholas I Goldenson, Saul Shiffman, Douglas Oliveri, Qiwei Liang, Ryan A Black","doi":"10.1080/1354750X.2024.2448493","DOIUrl":"10.1080/1354750X.2024.2448493","url":null,"abstract":"<p><strong>Introduction: </strong>Adults who switch from smoking cigarettes to use of electronic nicotine delivery systems (ENDS) may reduce their exposure to harmful and potentially harmful constituents (HPHCs). This study assessed changes in exposure to HPHCs, assessed <i>via</i> biomarkers of exposure (BOEs), among adults who switched to a new ENDS product.</p><p><strong>Methods: </strong>Adults who smoke cigarettes (<i>N</i> = 89) were randomized to: (1) switch completely to using JUUL2 Virginia Tobacco (<i>N</i> = 24) or Polar Menthol (<i>N</i> = 24); (2) continue smoking usual brand (UB) cigarettes (<i>N</i> = 21); or (3) abstain from all tobacco/nicotine products (<i>N</i> = 20) for 6 d. Changes in exposure to nicotine and 11 other HPHCs from Baseline to Day 6 were compared among study groups.</p><p><strong>Results: </strong>Changes in nicotine exposure did not significantly differ between JUUL2 and UB Cigarette groups (<i>p</i>s > 0.37). Among participants who switched completely to JUUL2 products, median percent reductions (Day 6-Baseline) in non-nicotine BOEs ranged from 65% to 94%, significantly greater than changes in the UB Cigarette group (<i>p</i>s < 0.001). None of the non-nicotine BOEs significantly differed between the JUUL2 groups and the Abstinence group (<i>p</i>s > 0.025).</p><p><strong>Conclusions: </strong>This randomized study demonstrates that adults who switch completely from smoking cigarettes to use of JUUL2 ENDS products substantially reduce their exposure to HPHCs associated with smoking-related diseases.</p><p><strong>International standard registered clinical trial number: </strong>ISRCTN27662176.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"77-87"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for <i>Staphylococcus aureus</i> colonization in a general emergency department patient cohort - results of an observational cohort study.","authors":"Dorothee Riedlinger, Fabian Holert, Petra Gastmeier, Axel Kola, Anna Slagman, Martin Möckel","doi":"10.1080/1354750X.2025.2451950","DOIUrl":"10.1080/1354750X.2025.2451950","url":null,"abstract":"<p><strong>Background: </strong>Testing for <i>Staphylococcus aureus</i> (SA) colonization in emergency department (ED) patients may guide prevention strategies against hospital acquired infections (HAIs). This study determined the prevalence of SA carriers in a general ED population, characterized the population, and identified predictors for SA colonization.</p><p><strong>Methods: </strong>A prospective monocentric observational cohort study in a tertiary care hospital collected nasopharyngeal swabs in 1000 adult patients. Polymerase chain reaction (PCR) testing for methicillin resistant and methicillin sensitive SA (MRSA/MSSA) was performed. Risk factor questionnaires and routine data from the clinical information system were captured. Descriptive statistics and binary logistic regression models were applied to report prevalence and outcomes and to identify predictors.</p><p><strong>Results: </strong>The prevalence for SA was 33.7% (<i>n</i> = 328; 95%-CI: 30.7-36.8): MSSA 30.9% (<i>n</i> = 301; 95%-CI: 28.0-34.0) and MRSA 2.8% (<i>n</i> = 27; 95%-CI: 1.8-4.0). Key predictors of SA colonization included having a catheter (OR 2.0, 95%-CI 1.0-4.0, <i>p</i> = 0.044) and requiring nursing care (OR 1.9, 95%-CI: 1.2-2.9, <i>p</i> = .007), even after adjusting for age and sex.</p><p><strong>Conclusion: </strong>Testing strategies for SA detection in ED need to focus on vulnerable populations with an elevated risk for HAIs and associated adverse outcomes. Individuals requiring nursing care could be a key target population for screening efforts.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"97-103"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}