外泌体hsa-miR-125b-5p和hsa-miR-320c作为哈萨克斯坦高氡地区非侵入性生物标志物的作用

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2025-01-17 DOI:10.1080/1354750X.2025.2456007

Akmaral Aripova, Assiya Kussainova, Milana Ibragimova, Olga Bulgakova, Rakhmetkazhi Bersimbaev

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引用次数: 0

摘要

背景：氡是一种放射性气体，是肺癌的重要危险因素，特别是在不吸烟者中。本研究检测了外泌体microRNAs （miRNAs）作为氡诱导效应的潜在生物标志物的表达。方法：对来自哈萨克斯坦氡高、低地区的109名参与者进行调查。采用real-time PCR定量外泌体hsa-miR-125b-5p和hsa-miR-320c水平。结果：结果显示，与对照组相比，暴露于高氡水平的参与者hsa-miR-125b-5p增加25.4倍，hsa-miR-320c减少12.5倍。生物信息学分析确定了与癌症发展有关的关键靶基因，如PRDM1和IRF4。结论：这些发现表明外泌体mirna可以作为氡暴露的非侵入性生物标志物，为氡诱发肺癌的早期诊断和监测提供了潜力。这项研究强调需要进一步的研究来验证这些mirna作为可靠的诊断工具。

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The role of exosomal hsa-miR-125b-5p and hsa-miR-320c as non-invasive biomarkers in high-radon areas of Kazakhstan.

Background: Radon, a radioactive gas, is a significant risk factor for lung cancer, especially in non-smokers. This study examines the expression of exosomal microRNAs (miRNAs) as potential biomarkers for radon-induced effects.

Methods: A total of 109 participants from high- and low-radon areas in Kazakhstan were included. Exosomal hsa-miR-125b-5p and hsa-miR-320c levels were quantified using real-time PCR.

Results: Results revealed a 25.4-fold increase in hsa-miR-125b-5p and a 12.5-fold decrease in hsa-miR-320c in participants exposed to high radon levels compared to controls. Bioinformatic analysis identified key target genes, such as PRDM1 and IRF4, which are implicated in cancer development.

Conclusion: These findings suggest that exosomal miRNAs could serve as non-invasive biomarkers for radon exposure, offering potential for early diagnosis and monitoring of radon-induced lung cancer. The study underscores the need for further research to validate these miRNAs as reliable diagnostic tools.

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.