Biomarkers最新文献

{"title":"Neuroprotective effects of magnesium against stress induced by hydrogen peroxide in Wistar rat.","authors":"Latifa Hajri,&nbsp;Haifa Othman,&nbsp;Soumaya Ghodbane,&nbsp;Sakly Mohsen,&nbsp;Hafedh Abdelmelek,&nbsp;Khemais Ben Rhouma,&nbsp;Mohamed Ammari","doi":"10.1080/1354750X.2023.2246104","DOIUrl":"10.1080/1354750X.2023.2246104","url":null,"abstract":"<p><strong>Introduction: </strong>Oxidative stress has been implicated in the pathogenesis of diverse disease states. The present study was designed to examine the effects of magnesium sulphate (MgSO₄) against hydrogen peroxide (H₂O₂) induced behaviour impairment and oxidative damage in rats.</p><p><strong>Material and methods: </strong>Eighteen rats were equally divided into three groups. The first group was kept as a control. In the second group, H₂O₂ was given in drinking water at 3% during 5 days. In the third group, rats were subjected to daily administration of H₂O₂ and MgSO₄ (100 mg/kg; b.w) for 5 days. Animals were subjected to behavioural tests (elevated plus maze and open field). At the end of experiment, brains were extracted for oxidative stress biomarkers assessment including levels of malondialdéhyde and hydrogen peroxide and activities of superoxide dismutase and catalase.</p><p><strong>Results: </strong>Our findings showed that H₂O₂ treated rat exhibited anxiogenic behaviour and the genesis of free radicals in the brain. Magnesium showed amelioration against oxidative stress and significant decrease in anxiety levels.</p><p><strong>Discussion and conclusion: </strong>Stress is a powerful process that disrupts brain homeostasis by inducing oxidative stress and its appear that magnesium may have potential therapeutic benefits by reducing oxidative stress and inducing anxiolytic effect.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"538-543"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10069687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential biomarkers in endometrial cancer: a narrative review.","authors":"Kareena Moar,&nbsp;Anuja Pant,&nbsp;Vikas Saini,&nbsp;Pawan Kumar Maurya","doi":"10.1080/1354750X.2023.2179114","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2179114","url":null,"abstract":"<p><strong>Context: </strong>Every year, approximately 0.4 million women suffer from endometrial cancer (EC) worldwide and it has become the most common gynecological malignancy. Almost 66% of EC cases are diagnosed at an early stage and can be cured by performing surgery while those at an advanced stage turns out to be fatal. Biomarkers of endometrial cancer would be very valuable for screening of women who are at high risk and in detecting the chance of recurrence of disease.</p><p><strong>Objective: </strong>The current article has reviewed studies published on expression of biomarkers and susceptibility to EC.</p><p><strong>Methods: </strong>Google Scholar and PubMed were used as searching platforms and we have majorly considered the literature from last 10 years.</p><p><strong>Results: </strong>Potential biomarkers of EC identified from various studies were summarised.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"358-371"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9589542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a urinary microRNA biomarker panel as a tool for early detection of prostate cancer in a Chinese population.","authors":"Nan Zhan,&nbsp;Jianfeng Wang,&nbsp;Shigeng Zhang,&nbsp;Huifeng Wu,&nbsp;Zhongyi Li,&nbsp;Maolin Hu","doi":"10.1080/1354750X.2023.2166587","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2166587","url":null,"abstract":"<p><strong>Introduction: </strong>Urinary microRNAs (miRNAs) may serve as promising biomarkers for non-invasive early detection of prostate cancer (PCa). We aimed to identify multi-miRNA urinary biomarker panel for early detection of PCa.</p><p><strong>Methods: </strong>Urine samples from 83 PCa patients and 88 healthy control subjects in a Chinese population were collected for miRNA profiling. The absolute expression of 360 unique miRNAs were measured in each sample using a highly sensitive and robust RT-qPCR workflow. Candidate urinary miRNA biomarkers were identified based on differential expression between PCa patients and healthy controls. Multi-miRNA biomarker panels were optimised for detection of PCa using three regression algorithms (Lasso, Stepwise, Exhaustive) to identify an optimal biomarker panel with best detection performance and least number of miRNAs.</p><p><strong>Results: </strong>A total of 312 miRNAs were detected in urine samples, 10 candidate urinary miRNA biomarkers differentially expressed between PCa and healthy samples were identified. A panel comprising these 10 miRNAs detected PCa with an area under the curve (AUC) of 0.738. Optimization of multi-miRNA panels resulted in a 6-miRNA biomarker panel (hsa-miR-375, hsa-miR-520d-5p, hsa-miR-199b-5p, hsa-miR-518e-5p, hsa-miR-31-3p and hsa-miR-4306) that had an AUC of 0.750.</p><p><strong>Conclusion: </strong>We identified a urinary miRNA biomarker panel for early detection of PCa in a Chinese population.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"372-378"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9643557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of serum procalcitonin and point-of-care multiplex PCR gastro-intestinal panel in acute diarrhoea or colitis in the emergency department.","authors":"Marta Cancella de Abreu,&nbsp;Clementine Cassard,&nbsp;Ilaria Cherubini,&nbsp;Enfel Houas,&nbsp;Agnès Dechartres,&nbsp;Pierre Hausfater","doi":"10.1080/1354750X.2023.2193356","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2193356","url":null,"abstract":"<p><strong>Introduction: </strong>Acute infectious diarrhoea is one of the most common diseases worldwide. Procalcitonin (PCT) is useful for antibiotic stewardship in lower respiratory tract infections but has been poorly studied in infectious diarrhoea. Our objective is to describe the PCT concentrations according to diarrhoea aetiology.</p><p><strong>Methods: </strong>This is a single-center prospective cohort study involving adults consulting the emergency department (ED) for an acute diarrhoea or colitis. Serum PCT was measured and a stool sample was tested with FilmArray® Gastro-Intestinal Panel. The primary endpoint is the PCT concentration according to each type of pathogen identified using Gastro-Intestinal-panel and/or stool cultures at ED admission.</p><p><strong>Results: </strong>125 patients were included: 80 had an acute infectious diarrhoea, 21 an acute colitis and 24 another illness causing diarrhoea. The median (interquartile ranges) PCT values (ng/ml) were 0.13 (0.08-0.28), 0.07 (0.06-0.54), 0.13 (0.09-0.26) and 0.05 (0.03-0.17), respectively if there was a bacteria (n = 41), parasite (n = 3), virus (n = 10) or no pathogen identified and 0.34 (0.13-1.03) if the diarrhoea was due to another illness (n = 24).</p><p><strong>Conclusion: </strong>In patients admitted to the ED with an acute infectious diarrhoea or acute colitis, PCT remained low when a bacteria was identified. It may not be informative in current practice to guide antibiotic therapy.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"396-400"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9960915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Golgi signalling proteins GOLPH3, MYO18A, PITPNC1 and RAB1B: implications in prognosis and survival outcomes of AML patients.","authors":"Yomna Ali,&nbsp;Sara M Radwan,&nbsp;Alia Saeed,&nbsp;Hala El-Mesallamy","doi":"10.1080/1354750X.2023.2191166","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2191166","url":null,"abstract":"<p><strong>Background: </strong>The role of different Golgi signalling proteins remains unexplored in the progression and spread of acute myeloid leukaemia (AML), whom all interact together in a way that facilitates proliferation and differentiation of myeloid lineage cells.</p><p><strong>Objective: </strong>Since Golgi apparatus acts as master brain in membrane trafficking and signalling events that affect cell polarity necessary for migration, division, or differentiation; this study aims to explore the association between signalling proteins and the diagnosis, prognosis, and survival of AML patients.</p><p><strong>Material and methods: </strong>This study comprised 70 newly diagnosed AML patients and 20 healthy controls to investigate the serum levels of signalling proteins; Golgi Phosphoprotein 3 (GOLPH3), Myosin 18A (MYO18A), Cytoplasmic Phosphatidylinositol Transfer Protein 1 (PITPNC1) and Ras-Associated Binding Protein 1B (RAB1B).</p><p><strong>Results: </strong>AML patients showed higher serum levels of GOLPH3, MYO18A, PITPNC1 and RAB1B when compared to control (<i>p</i> < 0.001). A significant negative correlation was found between the patients' overall survival and GOLPH3 (<i>p</i> = 0.001), MYO18A (<i>p</i> = 0.011), PITPNC1 (<i>p</i> = 0.001) and RAB1B (<i>p</i> = 0.042). Results were confirmed by Kaplen-Meier survival analysis showing lower survival estimates in patients with higher GOLPH3 (<i>p</i> = 0.014), MYO18A (<i>p</i> = 0.047), PITPNC1 (<i>p</i> = 0.008) and RAB1B (p = 0.033) serum levels.</p><p><strong>Conclusion: </strong>GOLPH3, MYO18A, PITPNC1 and RAB1B maybe promising diagnostic and prognostic biomarkers in AML patients.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"387-395"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives on cardiovascular biomarkers: one fits all biomarkers are out, personalization is in.","authors":"Martin Möckel","doi":"10.1080/1354750X.2023.2212913","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2212913","url":null,"abstract":"in this issue of Biomarkers, 8 exceptional abstracts of the “17th annual Biomarkers and Personalised Medicine in Cardiovascular disease Symposium” are published [https:// doi.org/10.1080/1354750X.2023.2205221]. these abstracts reflect current trends in cardiovascular biomarkers clinical needs and topics including markers of cardiac injury and infarction (abstracts no. 1, 2 and 6), of contrast nephropathy (abstract no. 3), of arrhythmias (abstract no. 4 and 8), acute heart failure (abstract no. 5) and infection (abstract no 7). it is noteworthy that despite the fact that the actual guidelines of the European Society of Cardiology (ESC) do not support other biomarkers than cardiac troponin for the assessment of patients with suspected acute coronary syndrome (aCS) (Collet et al., 2020) Cepin et al. (abstract no. 6) highlight with two instructive case reports that CK-MB may close unmet needs of high-sensitive troponin (hstn) although CK-MB is thought to be of no benefit any more (Jaffe et al., 2021). the authors highlight that in patients with concomitant disease like renal failure and in late presenters, CK-MB dynamics help to be more precise in individual decision making than cardiac troponin alone. Wong et al. (abstract 1) found that there are relevant “Ethnic differences in High-Sensitivity Cardiac troponin t among Patients with Chest Pain in the Emergency department” leading to under-diagnosis of acute myocardial infarction among asians. in summary, both abstracts challenge the “one fits all strategy” using hstn of the current ESC guideline, which has been criticized by others regarding different biomarkers before (Möckel et al, 2020). titus et al. (abstract no. 2) provide proof of concept using a transdermal wearable device to detect cardiac injury. the device was used in conjunction with a neural network. the system was able to differentiate patients with cardiac injury from normal subjects with a supreme C-statistic of 0.96 (Ci: 0.95 − 0.99). it is obvious that this approach has the potential to change the current standards of the evaluation of suspected aCS specifically in a community and emergency medical services setting. Potentially, unnecessary transportation can be avoided and a combination with telemedical services may form a “mobile emergency department”. the figure summarizes the potential future clinical impact of these results. Nevertheless, future research on these options should include the assessment of possible impact on actual standard pathways and on patient groups with no or limited access to digital devices as the elderly. these and the remaining abstracts provide unique and thought provoking insights in new biomarkers which support personalization of acute cardiovascular medicine. We are looking forward to publishing full length articles on these topics in the near future.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"353"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9997625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of MET gene somatic mutations in hepatocellular carcinoma of Egyptian patients using next-generation sequencing.","authors":"Ehab Ahmed,&nbsp;Nour El-Dien A,&nbsp;Salwa Sabet,&nbsp;Mohamed Khalifa,&nbsp;Manal El Hamshary","doi":"10.1080/1354750X.2023.2184497","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2184497","url":null,"abstract":"<p><strong>Introduction: </strong>Hepatocellular carcinoma (HCC) is the sixth most common type of cancer worldwide and fourth in Egypt. Liquid biopsy is important to get cell-tumour DNA (ctDNA), for subsequent utilisation as a biomarker for cancer diagnosis, prognosis, and treatment. In clinical oncology, ctDNA analysis is utilised in cancer screening.</p><p><strong>Methods: </strong>The collected 48 blood samples from HCC patients were classified according to Barcelona Clinic Liver Cancer (BCLC) staging, in addition to Hepatitis C Virus (HCV) group and normal group. After the liquid biopsy, ctDNA and genomic DNA (gDNA) of the same individual were extracted. Next-generation sequencing (NGS) was conducted using a Hot spot panel, and data analysis via different cancer databases was performed.</p><p><strong>Results: </strong>There were no significant differences in the detected mutation frequency between groups. The frequency of mutations was higher in ctDNA than in the gDNA samples from the same patients. Hence, it can be concluded that these mutations are somatic mutations, rather than germline mutations.</p><p><strong>Conclusion: </strong>Screening of the targeted genes such as c-MET for potential mutations is very important in the determination of the appropriate therapy. Therefore, it can be used as a biomarker in the prognosis of HCC. Such screenings are also of paramount importance in the development of personalised medicine.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"379-386"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9586854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vivo</i> assessment of inflammatory cytokines induced oxidative stress signalling, and troponin I gene dysregulation in cardiac tissue associated with chronic administration of boldenone and tramadol, alone or in combination.","authors":"Marwa E A El-Shamarka,&nbsp;Gihan F Asaad,&nbsp;Noha A Mowaad,&nbsp;Magy R Kozman","doi":"10.1080/1354750X.2023.2193357","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2193357","url":null,"abstract":"<p><strong>Introduction: </strong>The risk of cardiotoxicity is associated with the use of anabolic-androgenic steroids and analgesics, several deaths were attributed to such medications.</p><p><strong>Objectives: </strong>This study investigates the effects of boldenone (BOLD) and tramadol (TRAM) alone or in combination on the heart.</p><p><strong>Material and methods: </strong>Forty adult male rats were divided into four groups. Normal control group, BOLD (5 mg/kg, i.m.) per week, tramadol Hcl (TRAM) (20 mg/kg, i.p.) daily and a combination of BOLD (5 mg/kg) and TRAM (20 mg/kg), respectively for two months. Serum and cardiac tissue were extracted for determination of serum, aspartate aminotransferase (AST), creatine phosphokinase (CPK) and lipid profiles, tissue malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), tumour necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and histopathological examination. Troponin I gene expression was quantified in cardiac tissue using real-time polymerase chain reaction technique.</p><p><strong>Results: </strong>Groups received BOLD and TRAM alone and in combination showed elevated serum biochemical parameters (AST, CPK) and deviations in lipid profiles, elevation in oxidative and inflammatory parameters (MDA, NO, TNF-α and IL-6), and decrease in GSH and SOD, up-regulated cardiac troponin I as well as distorted cardiac histopathological pictures.</p><p><strong>Conclusion: </strong>The current study elucidated the risk of administration of these drugs for sustained periods as well as the marked detrimental effects of using these drugs in combination.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 4","pages":"401-408"},"PeriodicalIF":2.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-treatment systemic immune-inflammation index as a non-invasive biomarker for predicting clinical outcomes in patients with renal cell carcinoma: a meta-analysis of 20 studies.","authors":"Jun Xu,&nbsp;Junying Song,&nbsp;Zhenhua Yang,&nbsp;Jianguo Zhao,&nbsp;Jianfang Wang,&nbsp;Caiping Sun,&nbsp;Xiaoling Zhu","doi":"10.1080/1354750X.2023.2164906","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2164906","url":null,"abstract":"<p><strong>Introduction: </strong>To systematically assess the predictive significance of systemic immune-inflammation index (SII) in renal cell carcinoma (RCC).</p><p><strong>Methods: </strong>Relevant studies published before November 2022 were retrieved from public databases. Hazard ratio (HR), standardised mean difference (SMD) and relative risk (RR) were calculated to estimate associations of SII with prognosis, treatment responses and clinicopathological features.</p><p><strong>Results: </strong>Twenty studies involving 6887 patients were eligible. The meta-analysis results revealed a high SII level was associated with worse overall survival (HR: 1.45, p < 0.001), progression-free survival (HR: 1.63, p = 0.001), cancer-specific survival (HR: 1.86, p < 0.001), lower overall response rate (RR: 0.62, p = 0.003), disease control rate (RR: 0.69, p = 0.002), larger tumour size (SMD: 0.39, p = 0.001), poorer IMDC risk (RR: 7.09, p < 0.001), higher Fuhrman grade (RR: 1.54, p = 0.004), tumour stage (RR: 1.67, p = 0.045), the presence of distant metastasis (brain: RR, 2.04, p = 0.001; bone: RR, 1.33, p = 0.024) and tumour necrosis (RR: 1.57, p = 0.031). Subgroup analysis showed SII predicted OS and PFS for non-Asian, but CSS for both Asian and non-Asian populations.</p><p><strong>Conclusion: </strong>Pre-treatment SII may be a promising predictor of clinical outcomes for RCC patients.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 3","pages":"249-262"},"PeriodicalIF":2.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9765812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxic effect of carpet dust on the biochemical indices and histological structure of the lung in rats: the potential role of cytochrome P450 2E1 and extracellular signal-regulated kinase/mitogen-activated protein kinase pathways.","authors":"Abeer Salama,&nbsp;Rania Elgohary,&nbsp;Noha Mowaad,&nbsp;Doaa Sadek,&nbsp;Walaa Abdelhamid","doi":"10.1080/1354750X.2023.2164905","DOIUrl":"https://doi.org/10.1080/1354750X.2023.2164905","url":null,"abstract":"<p><p><b>Background:</b> Carpet dust exposure in the carpet industry causes various respiratory hazards that lead to permanent loss of lung function. This study investigated the potentially toxic effects of knotted and tufted carpet dust on rat lungs and the possible involvement of cytochrome P450 2E1 (CYP2E1) and extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathways in the induced toxicity, as well as histological changes in the lung induced by carpet dust.<b>Methods:</b> This study divided 48 adult rats into six groups: group I was the control group, group II (vehicle group) received phosphate buffer saline (50 µL/rat), groups III and IV received knotted dust (2.5 and 5 mg/kg, respectively), and groups V and VI received tufted dust (2.5 and 5 mg/kg, respectively). All treatments were intranasally administered once a day for 7 days.<b>Results:</b> Both dust types significantly decreased the lung content of GSH compared with the control. Significantly elevated malondialdehyde (MDA) and nitric oxide (NO) lung contents were observed with an increased CYP2E1, interleukin (IL)-6, nuclear factor kappa B (NF-κβ), and ERK/MAPK. The histological lung structure was moderately affected with a moderately increased number of CD68-positive macrophages in the lung parenchyma of knotted dust-exposed rats, whereas tufted dust exposure severely affected the lung tissue with significantly increased CD68-positive macrophages.<b>Conclusions:</b> Carpet dust exposure could induce oxidative stress and inflammatory response in the lung tissue <i>via</i> induction of CYP2E1 that stimulates ERK/MAPK signalling pathway proteins, resulting in elevated MDA, NO and IL-6 levels in the lung tissue with suppressed GSH content. Tufted dust could possess a more toxic response than knotted ones.</p>","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":"28 3","pages":"289-301"},"PeriodicalIF":2.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9389820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}