Arginase is upregulated in healthy women infected by oncogenic HPV types.

IF 2 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biomarkers Pub Date : 2023-12-01 Epub Date: 2023-12-11 DOI:10.1080/1354750X.2023.2273226
Moufida Souid, Jaweher Bday, Sameh Souissi, Randa Ghedira, Sallouha Gabbouj, Salwa Shini-Hadhri, Dhekra Toumi, Haifa Bergaoui, Ines Zouari, Raja Faleh, Abdelfatteh Zakhama, Elham Hassen
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引用次数: 0

Abstract

Introduction: The implication of arginase enzyme in Human Papillomavirus (HPV) infections has not been clearly elucidated. The present study investigates whether HPV infection is correlated with changes in plasmatic arginase activity and cervical ARG1 and ARG2 mRNA expression among infected women negative for intraepithelial lesions (NIL).

Materiel and methods: The present study included 300 women. The plasmatic arginase activity was evaluated by a colorimetric assay. Cervical HPV was detected by real-time PCR. The circulating viral load and ARG1 and ARG2 mRNA expression quantification were performed by quantitative real-time PCR.

Results: A significant increase in plasma arginase activity and ARG1 and ARG2 mRNA expression levels in cervical cells was observed among HPV-positive women compared to the HPV-negative group. The highest levels were significantly associated with oncogenic HPV, and increased arginase activity was associated with a high HPV circulating viral load. Moreover, the highest levels of arginase activity were observed in oncogenic HPV-positive inflammatory smears.

Discussion: These data suggest that HPV could modulate arginase activity and expression, which may restrict arginine bioavailability and inhibit this amino acid's antiviral properties.

Conclusion: Our findings revealed that arginase activity and isoform gene expression were upregulated in women with HPV infection, particularly the oncogenic HPV types.

Arginase在感染致癌HPV类型的健康女性中上调。
引言:精氨酸酶在人乳头瘤病毒(HPV)感染中的作用尚未明确阐明。本研究调查了在上皮内病变(NIL)阴性的感染女性中,HPV感染是否与血浆精氨酸酶活性和宫颈ARG1和ARG2 mRNA表达的变化相关。材料和方法:本研究包括300名女性。用比色法测定血浆精氨酸酶活性。实时聚合酶链式反应检测宫颈HPV。通过定量实时PCR对循环病毒载量和ARG1和ARG2 mRNA表达进行定量。结果:与HPV阴性组相比,HPV阳性女性的血浆精氨酸酶活性以及宫颈细胞中ARG1和ARG2 mRNA表达水平显著增加。最高水平与致癌HPV显著相关,精氨酸酶活性增加与高HPV循环病毒载量相关。此外,在致癌的HPV阳性炎症涂片中观察到最高水平的精氨酸酶活性。讨论:这些数据表明,HPV可以调节精氨酸酶的活性和表达,这可能限制精氨酸的生物利用度并抑制这种氨基酸的抗病毒特性。结论:我们的研究结果表明,精氨酸酶活性和亚型基因表达在HPV感染女性中上调,尤其是致癌HPV类型。
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来源期刊
Biomarkers
Biomarkers 医学-毒理学
CiteScore
5.00
自引率
3.80%
发文量
140
审稿时长
3 months
期刊介绍: The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.
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