Biomarkers最新文献_第2页

Dielectric ionic conductivity as a biomarker for lead chelation using nano-ZnO/CMC in albino rats. 电介质离子电导率作为纳米zno /CMC在白化病大鼠中铅螯合的生物标志物。

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-10 DOI: 10.1080/1354750X.2026.2620721

Faith Geoffrey, Victor M Ahur, Solomon T Agu, Terver Sombo, Richard O Ocaya, Iorkyaa Ahemen

{"title":"Dielectric ionic conductivity as a biomarker for lead chelation using nano-ZnO/CMC in albino rats.","authors":"Faith Geoffrey, Victor M Ahur, Solomon T Agu, Terver Sombo, Richard O Ocaya, Iorkyaa Ahemen","doi":"10.1080/1354750X.2026.2620721","DOIUrl":"10.1080/1354750X.2026.2620721","url":null,"abstract":"Background: This study evaluates dielectric conductivity as a diagnostic tool for assessing lead (Pb) levels in albino rats. It also investigated the ameliorative potentials of nano-ZnO-capped sodium carboxymethyl cellulose (nZnO/CMC).Methods: The synthesis of nZnO/CMC was carried out using the co-precipitation technique. The average crystallite size obtained from X-ray diffraction measurements is 33 nm. The effect of Pb induced toxicity was evaluated using four groups of six albino rats each, administered with Pb for 61 days. Haematological results show decreasing trends in packed cell volume, red blood and white blood cell counts, and haemoglobin concentration with Pb administration, indicating anaemia.Results: Upon nZnO/CMC administration, both haematological and erythrocyte surface sialic acids parameters were restored, suggesting that nZnO/CMC has Pb chelating capabilities. Toxicity studies revealed nZnO/CMC to be non-toxic to tissues below 2000 mg/kg body weight per os. Dielectric conductivity of normal and exposed blood samples measured between 200 Hz and 4 MHz shows dominance of ionic conductivity; highest for Pb-exposed samples and lowest for the normal/control sample. It also showed a decreasing trend for samples treated with nZnO/CMC at 100 and 200 mg/kg.Conclusions: This work strongly correlates changes in dielectric ionic conductivity with haematological/Pb exposure concentration, suggesting that dielectric ionic conductivity of blood is a promising biomarker for Pb-exposed animals.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"1-13"},"PeriodicalIF":1.9,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering Clinically Relevant Breast Cancer Subtypes Biomarkers Using Integrative Bioinformatics and Machine Learning Approaches. 利用综合生物信息学和机器学习方法揭示临床相关的乳腺癌亚型生物标志物。

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-09 DOI: 10.1080/1354750X.2026.2633406

Prashansha Goel, Nilofer Shaikh

{"title":"Uncovering Clinically Relevant Breast Cancer Subtypes Biomarkers Using Integrative Bioinformatics and Machine Learning Approaches.","authors":"Prashansha Goel, Nilofer Shaikh","doi":"10.1080/1354750X.2026.2633406","DOIUrl":"https://doi.org/10.1080/1354750X.2026.2633406","url":null,"abstract":"A precise diagnosis and customized treatment become more difficult by the genomic heterogeneity of breast cancer (BRCA). In order to examine gene expression data from two separate Gene Expression Omnibus (GEO) microarray datasets, we used a integrative approach in this study that combined bioinformatics and machine learning. We were able to distinguish between universal and subtype-specific transcriptome patterns by identifying both common and subtype-specific differentially expressed genes (DEGs) using dual-level differential expression analysis. Functional enrichment analysis and the creation of protein-protein interaction networks identified important hub genes, including TPM3, MYLK, and COL17A1, which showed substantial dysregulation and were linked to high mutation rates and a bad prognosis. Survival analyses, which identified COL17A1 as a predictive predictor for the general population and MYLK for the Luminal B subtype, highlighted the clinical significance of these hub genes. We used both Random Forest and K-Nearest Neighbors classifiers to ensure robust biomarker identification. In the analysis, we prioritized 35 model-agnostic biomarkers that performed well in subtype categorization, such as PNMT and KRTAP10-8. This dual-model approach improved the reliability of biomarker identification while reducing model-specific biases. These results set the stage for early identification, more accurate subtype classification, and possible therapeutic targeting in breast cancer.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression levels of miR-146a-5p, miR-155-5p and the pro-inflammatory cytokine IL-8 in pregnant women with anti-phospholipid syndrome. miR-146a-5p、miR-155-5p及促炎细胞因子IL-8在抗磷脂综合征孕妇中的表达水平

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-04 DOI: 10.1080/1354750X.2026.2636140

Mai M Shaker, Nesma M Elaraby, Taghreed A Shalabi

引用次数: 0

Cerebral ischemia-reperfusion induced cardiac injury in rats: involvement of oxidative stress, apoptosis, TLR4, nF-κB and HSP70: the rescue effect of losartan. 脑缺血再灌注大鼠心脏损伤：氧化应激、细胞凋亡、TLR4、NF-κB、HSP70的参与：氯沙坦的拯救作用

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-04 DOI: 10.1080/1354750X.2026.2634890

Yasaman Shakouri, Hamid Soraya, Roya Naderi

{"title":"Cerebral ischemia-reperfusion induced cardiac injury in rats: involvement of oxidative stress, apoptosis, TLR4, nF-κB and HSP70: the rescue effect of losartan.","authors":"Yasaman Shakouri, Hamid Soraya, Roya Naderi","doi":"10.1080/1354750X.2026.2634890","DOIUrl":"10.1080/1354750X.2026.2634890","url":null,"abstract":"Background: This study aimed to explore the effect of cerebral ischemia reperfusion and losartan on oxidative stress, inflammation, apoptosis and its molecular mediators in the rat heart.Methods: The groups were as follows (n = 6): 1) sham 2) ischemia-reperfusion (IR, 20 min ischemia, 24 hours reperfusion), 3) ischemia-reperfusion +losartan (IR+LOS, 3 mg/kg, ip, 1 hour before ischemia).Results: In IR animals' heart Malondialdehyde (MDA) and Total oxidative status (TOS) increased while Total antioxidant capacity (TAC) and Superoxide dismutase (SOD) concentration decreased compared to the sham rats. Cerebral IR increased apoptotic index evidenced by increased cytochrome c and c-caspase3/p-caspase3 ratio, as well as elevated Toll-like receptor4 (TLR4), Nuclear factor kappa B (NF-κB) and Heat Shock Protein70 (HSP70) in the heart tissue. These alterations could be mitigated by losartan. TLR4 is positively correlated with NF-κB, r = 0.61; MDA, r = 0.64; TOS, r = 0.59 (p < 0.01), cytochrome c, r = 0.83; c-caspase3/p-caspase3, r = 0.72 (p < 0.001) and negatively correlated with SOD, r = -0.73 and TAC, r = -0.83 (p < 0.001).Conclusion: In all, cerebral IR increased cardiac oxidative stress and apoptosis which positively correlates with TLR4/NF-κB pathway. Additionally, losartan can be considered as a potential target for preventing cardiac injury following stroke.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"1-8"},"PeriodicalIF":1.9,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early impact of lenvatinib on liver function and its prognostic significance: a single-center retrospective study. Lenvatinib对肝功能的早期影响及其预后意义：一项单中心回顾性研究。

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-11 DOI: 10.1080/1354750X.2026.2628045

Pei Wang, Shuheng Liu, Xiaoxia Lu

{"title":"Early impact of lenvatinib on liver function and its prognostic significance: a single-center retrospective study.","authors":"Pei Wang, Shuheng Liu, Xiaoxia Lu","doi":"10.1080/1354750X.2026.2628045","DOIUrl":"10.1080/1354750X.2026.2628045","url":null,"abstract":"Objective: Lenvatinib shows efficacy for tumor response and survival in patients with advanced hepatocellular carcinoma (HCC). We aim to assess the effect of lenvatinib on liver function.Methods: This single-center retrospective cohort study included 40 patients with advanced HCC who received ≥ 2 months of lenvatinib treatment from January 2020 to January 2024 and had at least one efficacy and safety assessment.Results: The ALBI score showed a slight but significant increase of 0.112 points, from -2.517 at baseline to -2.405 following 2-month of treatment (P < 0.05). With a median follow-up of 18.5 months, the median overall survival was 22.8 months (95% CI: 16.3-29.3), and the median progression-free survival was 12.7 months (95% CI: 9.1-16.3). Both COX regression and Kaplan-Meier analyses indicated that impaired baseline liver function was associated with adverse clinical outcomes. Additionally, treatment response emerged as an independent prognostic factor.Conclusions: The baseline ALBI score serves as a key prognostic marker, highlighting the necessity for meticulous monitoring of hepatic function, particularly in patients with compromised baseline liver reserve.Limitations: The limitations of this study include its retrospective design and the potential for selection bias arising from the requirement for at least 2 months of treatment.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"132-140"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Val66Met polymorphism in the brain-derived neurotrophic factor (BDNF) gene and its association with select anxiety-related disorders: an updated systematic review and meta-analysis. 脑源性神经营养因子（BDNF）基因Val66Met多态性及其与特定焦虑相关疾病的关联：一项最新的系统综述和荟萃分析

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-16 DOI: 10.1080/1354750X.2026.2628035

Raphael Enrique Tiongco, Ma Agatha Anne Guintu, Neil David Cayanan, Michael John Dominguez, Maria Ruth Pineda-Cortel

{"title":"Val66Met polymorphism in the brain-derived neurotrophic factor (BDNF) gene and its association with select anxiety-related disorders: an updated systematic review and meta-analysis.","authors":"Raphael Enrique Tiongco, Ma Agatha Anne Guintu, Neil David Cayanan, Michael John Dominguez, Maria Ruth Pineda-Cortel","doi":"10.1080/1354750X.2026.2628035","DOIUrl":"10.1080/1354750X.2026.2628035","url":null,"abstract":"Background: Conflicting results on the association of the Val66Met polymorphism in the BDNF gene with anxiety-related disorders are observed from previous studies and meta-analyses. We performed an updated meta-analysis to obtain more precise estimates and add additional analyses not performed by previous reviews.Methods: Using combinations of various key terms, articles in PubMed, Google Scholar, and Web of Science, written in English were collected until October 31, 2024. Data were extracted independently by two authors and analyzed using Review Manager 5.4.Results: Fifteen studies that are compliant with the HWE, providing a total of 14,184 participants were included in this meta-analysis after applying predefined inclusion/exclusion criteria based on study design, DSM-based diagnosis, and availability of genotype counts. Most pooled models demonstrated low to moderate heterogeneity with significant associations in the recessive model only. In the subgroup analysis, a significant effect was observed in the PD-uncategorized cohort. The Met/Met genotype demonstrated a suggestive association with increased susceptibility to panic disorder.Conclusion: Our updated meta-analysis suggests that the Met/Met genotype of the BDNF Val66Met polymorphism may increase susceptibility to PD under a recessive genetic model; however, this evidence remains preliminary.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"122-131"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a potential relationship between pigmented villonodular synovitis and atherosclerosis using comprehensive bioinformatics analyses. 利用综合生物信息学分析鉴定色素绒毛结节性滑膜炎与动脉粥样硬化之间的潜在关系。

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1080/1354750X.2026.2625237

Shuilin Chen, Honglei Jia, Guihao Zheng, Meifeng Lu, Yulong Ouyang, Xiangwei Fan, Guicai Sun

{"title":"Identification of a potential relationship between pigmented villonodular synovitis and atherosclerosis using comprehensive bioinformatics analyses.","authors":"Shuilin Chen, Honglei Jia, Guihao Zheng, Meifeng Lu, Yulong Ouyang, Xiangwei Fan, Guicai Sun","doi":"10.1080/1354750X.2026.2625237","DOIUrl":"10.1080/1354750X.2026.2625237","url":null,"abstract":"Background: This study investigates the pathogenesis of Pigmented Villonodular Synovitis (PVNS) using bioinformatics approaches shared with atherosclerosis (AS).Methods: Common genes from GSE3698 and GSE28829 were identified. A PPI network was constructed using the STRING database and analyzed with Cytoscape, MCODE, and cytoHubba to determine hub genes. These underwent GO and KEGG enrichment analysis. Candidate genes were identified by integrating cytoHubba and machine learning, and validated via immunohistochemistry and the GSE100927 dataset. Immune infiltration in PVNS and its association with candidate genes were examined, followed by scRNA-seq analysis using GSE155527.Results: Analysis of 43 shared genes implicated immune responses in both diseases. CSF2RB was identified as a key candidate gene through PPI, machine learning, and immunohistochemistry. Immune infiltration confirmed immune dysregulation and linked CSF2RB to multiple immune cells. scRNA-seq revealed increased macrophages, T cells, and endothelial cells in PVNS, with CSF2RB highly expressed in macrophages and mast cells.Conclusion: CSF2RB may serve as a diagnostic and therapeutic target for PVNS. scRNA-seq indicates disrupted immune cell composition in PVNS, characterized by significant increases in macrophages and T cells.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"112-121"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of inflammatory parameters and ferritin as prognostic factors in non-small cell lung cancer patients receiving nivolumab immunotherapy. 接受纳武单抗免疫治疗的非小细胞肺癌患者炎症参数和铁蛋白作为预后因素的评估

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-13 DOI: 10.1080/1354750X.2026.2621291

İsmail Beypınar, Onur Yazdan Balçık, Semiha Urvay, Müslih Ürün, Berrak Erçek, Hacer Demir, Canan Yıldız, Murat Araz, Ahmet Oruç, Yusuf İlhan, Utku Özilice, Aziz Kurtulus

{"title":"Evaluation of inflammatory parameters and ferritin as prognostic factors in non-small cell lung cancer patients receiving nivolumab immunotherapy.","authors":"İsmail Beypınar, Onur Yazdan Balçık, Semiha Urvay, Müslih Ürün, Berrak Erçek, Hacer Demir, Canan Yıldız, Murat Araz, Ahmet Oruç, Yusuf İlhan, Utku Özilice, Aziz Kurtulus","doi":"10.1080/1354750X.2026.2621291","DOIUrl":"10.1080/1354750X.2026.2621291","url":null,"abstract":"Aim: Inflammation and immune dysfunction significantly impact cancer progression and treatment responses. This retrospective study investigated inflammatory parameters and ferritin in predicting immunotherapy response in non-small cell lung cancer (NSCLC) patients.Methods: The study included 199 patients with NSCLC who received nivolumab between 2018 and 2024 at five medical centers. Various inflammatory markers were also evaluated. Ferritin levels at diagnosis and pretreatment were also evaluated.Results: ROC curve analyses showed ferritin delta had high prognostic performance for PFS and OS, with AUC values of 0.70 and 0.73. PIV and PNI were significantly associated with PFS and OS. In Kaplan-Meier analyses, PNI was the most consistent prognostic factor. Low PNI (≤43.5) significantly associated with shorter OS (5.0 vs. 15.0 months, p = 0.001) and shorter PFS (4.0 vs. 8.0 months, p = 0.002). High mGPS (score 2) and elevated PIV showed significant prognostic value. In multivariate Cox regression, PNI demonstrated independent prognostic significance. Objective response rate was the strongest prognostic factor for PFS (HR = 0.188, 95%CI: 0.114-0.309, p < 0.001).Conclusion: These findings highlight the prognostic value of inflammatory and nutritional markers in patients with NSCLC receiving immunotherapy. PNI demonstrated the most consistent prognostic value across multiple analytical approaches and maintained significance in the multivariate analysis.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"91-100"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146040317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile gas exposure correlates with self-reported liver condition: insights from NHANES 2009-2018 and Mendelian randomization studies. 挥发性气体暴露与自我报告的肝脏状况相关：来自NHANES 2009-2018和孟德尔随机化研究的见解

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1080/1354750X.2026.2623470

Yuyun Jia, Yanping Cao, Qin Yin, Xueqian Li, Xiu Wen

{"title":"Volatile gas exposure correlates with self-reported liver condition: insights from NHANES 2009-2018 and Mendelian randomization studies.","authors":"Yuyun Jia, Yanping Cao, Qin Yin, Xueqian Li, Xiu Wen","doi":"10.1080/1354750X.2026.2623470","DOIUrl":"10.1080/1354750X.2026.2623470","url":null,"abstract":"Introduction: Volatile gas exposure and self-reported liver conditions share pathophysiologic risk factors, but their exact association remains unclear.Methods: We used a weighted multivariable-adjusted logistic regression model, using data from the National Health and Nutrition Examination Survey (NHANES) 2009-2018. Integrative analyses of Non-alcoholic fatty liver disease (NAFLD), liver fibrosis, liver cirrhosis GWAS summaries and blood expression quantitative trait loci (eQTLs) were conducted via summary data-based MR (SMR) and colocalization analysis to prioritize putative blood smoking-related genes and their associations with each liver condition risk. Finally, we further verified these gene-disease causal links through MR and colocalization analysis.Results: 12,099 NHANES participants were included. Male sex, 46-75 years, and smoke exposure correlated positively with LC; age and smoke exposure remained positively associated with LC incidence after adjustment, while non-Hispanic Black was a LC protective factor. SMR identified three blood-derived candidate genes: RGPD8 (Beta = -0.207), COX6B2 (Beta = -0.567), DNAJC27 (Beta = 0.859). MR and colocalization confirmed their associations with cirrhosis, fibrosis, and NAFLD, respectively.Conclusion: This study confirms a causal link between smoke exposure and increased LC risk, identifies novel gene-disease associations, and provides cross-disciplinary insights for targeted LC prevention and personalized research.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"101-111"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146050392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating a data-driven approach to biomarker discovery for tumor-targeted imaging in epithelial ovarian cancer. 评估一种数据驱动的方法来发现生物标志物，用于上皮性卵巢癌的肿瘤靶向成像。

IF 1.9 4区医学

Biomarkers Pub Date : 2026-03-01 Epub Date: 2026-02-16 DOI: 10.1080/1354750X.2026.2620723

Lysanne D A N de Muynck, Peter J K Kuppen, Eva M de Ronde, Tom B Kuipers, Hailiang Mei, Tjalling Bosse, Alexander L Vahrmeijer, Katja N Gaarenstroom, Inge T A Peters

{"title":"Evaluating a data-driven approach to biomarker discovery for tumor-targeted imaging in epithelial ovarian cancer.","authors":"Lysanne D A N de Muynck, Peter J K Kuppen, Eva M de Ronde, Tom B Kuipers, Hailiang Mei, Tjalling Bosse, Alexander L Vahrmeijer, Katja N Gaarenstroom, Inge T A Peters","doi":"10.1080/1354750X.2026.2620723","DOIUrl":"10.1080/1354750X.2026.2620723","url":null,"abstract":"Introduction: In epithelial ovarian cancer (EOC), surgical outcome is the strongest prognostic factor for survival. However, estimating intra-abdominal tumor burden to plan optimal treatment strategies remains challenging. Moreover, metastases can remain undetected during surgery via visual and tactile inspection. Tumor-targeted molecular imaging has the potential to improve tumor cell identification pre- and intraoperatively. While targeting folate receptor-alpha (FRα) shows promise, other specific biomarkers are needed.Methods: This study evaluates a novel, data-driven approach using RNA expression data to identify new target proteins for tumor-targeted imaging in EOC. A knowledge platform was utilized to search omics-databases for membrane proteins expressed in EOC but absent or minimally expressed in surrounding tumor-negative and inflammatory cells.Results: Differential gene expression analysis identified highly expressed genes, which were validated through immunohistochemistry. Two new genes were identified: VTCN1 and AQP5, encoding for proteins B7-H4 and AQP5, respectively. Immunohistochemical validation showed that B7-H4 expression aligned with RNA levels, indicating its potential as a new target. In contrast, there was a discrepancy in AQP5 expression at the protein level compared to its gene counterpart.Discussion: While this approach was valuable in identifying novel targets for tumor targeted imaging of EOC, immunohistochemistry or cell studies remain imperative for validation of RNA expression results.","PeriodicalId":8921,"journal":{"name":"Biomarkers","volume":" ","pages":"80-90"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146050318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0