Bioengineered最新文献_第2页

Statement of Retraction: Ephedrine alleviates middle cerebral artery occlusion-induced neurological deficits and hippocampal neuronal damage in rats by activating PI3K/AKT signaling pathway. 撤回声明：麻黄碱通过激活PI3K/AKT信号通路减轻大鼠大脑中动脉闭塞引起的神经功能缺损和海马神经元损伤。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-06-25 DOI: 10.1080/21655979.2025.2515739

引用次数: 0

Statement of Retraction: The LOXL1 antisense RNA 1 (LOXL1-AS1)/microRNA-423-5p (miR-423-5p)/ectodermal-neural cortex 1 (ENC1) axis promotes cervical cancer through the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway. 结论：LOXL1反义RNA 1 (LOXL1- as1)/microRNA-423-5p (miR-423-5p)/外胚层-神经皮质1 （ENC1）轴通过丝裂原活化蛋白激酶(MEK)/细胞外信号调节激酶（ERK）通路促进宫颈癌。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-04-22 DOI: 10.1080/21655979.2025.2496006

引用次数: 0

A novel mannan-specific chimeric antigen receptor M-CAR redirects T cells to interact with Candida spp. hyphae and Rhizopus oryzae spores. 一种新的甘露聚糖特异性嵌合抗原受体M-CAR重定向T细胞与念珠菌菌丝和米根霉孢子相互作用。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-02-01 DOI: 10.1080/21655979.2025.2458786

Júlia Garcia Guimarães, Gabriela Yamazaki de Campos, Michele Procópio Machado, Patrícia Kellen Martins Oliveira Brito, Thaila Fernanda Dos Reis, Gustavo Henrique Goldman, Patricia Vianna Bonini Palma, Thais Fernanda de Campos Fraga-Silva, Daniela Cardoso Umbelino Cavallin, James Venturini, Thiago Aparecido da Silva

{"title":"A novel mannan-specific chimeric antigen receptor M-CAR redirects T cells to interact with Candida spp. hyphae and Rhizopus oryzae spores.","authors":"Júlia Garcia Guimarães, Gabriela Yamazaki de Campos, Michele Procópio Machado, Patrícia Kellen Martins Oliveira Brito, Thaila Fernanda Dos Reis, Gustavo Henrique Goldman, Patricia Vianna Bonini Palma, Thais Fernanda de Campos Fraga-Silva, Daniela Cardoso Umbelino Cavallin, James Venturini, Thiago Aparecido da Silva","doi":"10.1080/21655979.2025.2458786","DOIUrl":"10.1080/21655979.2025.2458786","url":null,"abstract":"Invasive fungal infections (IFIs) are responsible for elevated rates of morbidity and mortality, causing around of 1.5 million deaths annually worldwide. One of the main causative agents of IFIs is Candida albicans, and non-albicans Candida species have emerged as a spreading global public health concernment. Furthermore, COVID-19 has contributed to a boost in the incidence of IFIs, such as mucormycosis, in which Rhizopus oryzae is the most prevalent causative agent. The effector host immune response against IFIs depends on the activity of T cells, which are susceptible to the regulatory effects triggered by fungal virulence factors. The fungal cell wall plays a crucial role as a virulence factor, and its remodeling compromises the development of a specific T-cell response. The redirection of Jurkat T cells to target Candida spp. by recognizing targets expressed on the fungal cell wall can be facilitated using chimeric antigen receptor (CAR) technology. This study generated an M-CAR that contains an scFv with specificity to α-1,6 mannose backbone of fungal mannan, and the expression of M-CAR on the surface of modified Jurkat cells triggered a strong activation against Candida albicans (hyphae form), Candida tropicalis (hyphae form), Candida parapsilosis (pseudohyphal form), and Candida glabrata (yeast form). Moreover, M-CAR Jurkat cells recognized Rhizopus oryzae spores, which induced high expression of cell activation markers. Thus, a novel Mannan-specific CAR enabled strong signal transduction in modified Jurkat cells in the presence of Candida spp. or R. oryzae.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2458786"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular signaling pathways in osteoarthritis and biomaterials for cartilage regeneration: a review. 骨关节炎分子信号通路及软骨再生生物材料研究进展。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-05-07 DOI: 10.1080/21655979.2025.2501880

Samson Prince Hiruthyaswamy, Arohi Bose, Ayushi Upadhyay, Tiasa Raha, Shangomitra Bhattacharjee, Isheeta Singha, Swati Ray, Nazarene Marylene Nicky Macarius, Pragasam Viswanathan, Kanagavel Deepankumar

{"title":"Molecular signaling pathways in osteoarthritis and biomaterials for cartilage regeneration: a review.","authors":"Samson Prince Hiruthyaswamy, Arohi Bose, Ayushi Upadhyay, Tiasa Raha, Shangomitra Bhattacharjee, Isheeta Singha, Swati Ray, Nazarene Marylene Nicky Macarius, Pragasam Viswanathan, Kanagavel Deepankumar","doi":"10.1080/21655979.2025.2501880","DOIUrl":"10.1080/21655979.2025.2501880","url":null,"abstract":"Osteoarthritis is a prevalent degenerative joint disease characterized by cartilage degradation, synovial inflammation, and subchondral bone alterations, leading to chronic pain and joint dysfunction. Conventional treatments provide symptomatic relief but fail to halt disease progression. Recent advancements in biomaterials, molecular signaling modulation, and gene-editing technologies offer promising therapeutic strategies. This review explores key molecular pathways implicated in osteoarthritis, including fibroblast growth factor, phosphoinositide 3-kinase/Akt, and bone morphogenetic protein signaling, highlighting their roles in chondrocyte survival, extracellular matrix remodeling, and inflammation. Biomaterial-based interventions such as hydrogels, nanoparticles, and chitosan-based scaffolds have demonstrated potential in enhancing cartilage regeneration and targeted drug delivery. Furthermore, CRISPR/Cas9 gene editing holds promise in modifying osteoarthritis-related genes to restore cartilage integrity. The integration of regenerative biomaterials with precision medicine and molecular therapies represents a novel approach for mitigating osteoarthritis progression. Future research should focus on optimizing biomaterial properties, refining gene-editing efficiency, and developing personalized therapeutic strategies. The convergence of bioengineering and molecular science offers new hope for improving joint function and patient quality of life in osteoarthritis management.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2501880"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring metal bioaccumulation ability of boreal white-rot fungi on fiberbank material. 探讨北方白腐菌在纤维库材料上的金属生物富集能力。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-05-26 DOI: 10.1080/21655979.2025.2507539

Burcu Hacıoğlu, Gabriela Paladino, Mattias Edman, Alireza Eivazi, Erik Hedenström

{"title":"Exploring metal bioaccumulation ability of boreal white-rot fungi on fiberbank material.","authors":"Burcu Hacıoğlu, Gabriela Paladino, Mattias Edman, Alireza Eivazi, Erik Hedenström","doi":"10.1080/21655979.2025.2507539","DOIUrl":"10.1080/21655979.2025.2507539","url":null,"abstract":"Fiberbanks are organic-rich sediment deposits in aquatic environments, primarily formed through historical pulp and paper mill activities. These deposits consist of wood-derived fibrous materials and are contaminated with potentially toxic elements (PTEs) such as vanadium, chromium, cobalt, nickel, copper, zinc, arsenic, cadmium, and lead. The leaching of these contaminants into surrounding waters poses significant environmental and health risks, impacting aquatic ecosystems and potentially entering the food chain. Effective remediation of fiberbanks is crucial, particularly in Sweden and other regions with extensive wood-pulping industries. This study aims to evaluate the bioaccumulation capacities of 26 native Swedish white-rot fungi (WRF) species for the remediation of PTEs in fiberbank material. Fiberbank samples were collected from Sundsvall's Bay in the Baltic Sea, while the fungal species were isolated from boreal forests in Västernorrland, Sweden. The fungi were cultured on Hagem agar medium with sterilized fiberbank material as the substrate. After two months, fungal biomass was analyzed for PTE uptake using inductively coupled plasma-mass spectrometry (ICP-MS). The results revealed significant variability (p < 0.001) in PTE uptake among fungal species. Phlebia tremellosa consistently demonstrated the highest bioconcentration factors for analyzed elements, with values for V (0.39), Cr (0.10), Co (1.81), Cu (1.54), Pb (1.65), Ni (1.28), As (0.83), Zn (3.61), and Cd (5.56). Other species, including Laetiporus sulphureus (0.09-4.78), Hymenochaete tabacina (0.08-4.52), and Diplomitoporus crustulinus (0.08-4.48), also exhibited significant bioremediation potential. These findings highlight the potential of native WRF species for PTEs remediation in fiberbanks and provide a foundation for mycoremediation strategies in contaminated environments.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2507539"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the mechanisms of supplemented CO₂ in enhancing methane production in anaerobic digestion process, a review. 探讨在厌氧消化过程中补充CO2提高甲烷产量的机制。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-07-22 DOI: 10.1080/21655979.2025.2531667

Wahyunanto Agung Nugroho, Ni'matul Izza, Ummul Hasanah Hj Hassan, Ahmad A Alsaigh, Yusuf Wibisono

{"title":"Exploring the mechanisms of supplemented CO2 in enhancing methane production in anaerobic digestion process, a review.","authors":"Wahyunanto Agung Nugroho, Ni'matul Izza, Ummul Hasanah Hj Hassan, Ahmad A Alsaigh, Yusuf Wibisono","doi":"10.1080/21655979.2025.2531667","DOIUrl":"10.1080/21655979.2025.2531667","url":null,"abstract":"Anaerobic digestion (AD) is a sustainable technology that converts organic waste into renewable energy while reducing greenhouse gas emissions. Recent studies suggest that adding CO₂ to the AD process can improve methane production through different mechanisms. This review examines four key ways CO₂ supplementation can enhance methane yield: (1) direct conversion of CO₂ into acetate by homoacetogens, (2) direct methanation of CO₂ by hydrogenotrophic methanogens, (3) improved breakdown of organic material due to higher enzyme activity, and (4) better digester conditions through pH regulation and reduced ammonia toxicity. By analyzing microbial interactions and process improvements, this paper highlights knowledge gaps and the need for further research to optimize CO₂ addition in different operational settings. These findings are expected to contribute to the development of cost-effective and efficient AD systems that support energy recovery and environmental sustainability.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2531667"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biorefining spent substrates of shiitake (Lentinula edodes) and oyster mushroom (Pleurotus ostreatus): enzymatic saccharification of cellulose and xylan, with lignin recovery from residues. 香菇（Lentinula edodes）和平菇（Pleurotus ostreatus）废底物的生物精制：纤维素和木聚糖的酶糖化，从残留物中回收木质素。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-07-30 DOI: 10.1080/21655979.2025.2536443

Sarah J Klausen, Luis A Romero-Soto, Ayesha Liaqat, Zahra Dehghanmanshadi, Knut O Strætkvern, Shaojun Xiong, Carlos Martín

{"title":"Biorefining spent substrates of shiitake (Lentinula edodes) and oyster mushroom (Pleurotus ostreatus): enzymatic saccharification of cellulose and xylan, with lignin recovery from residues.","authors":"Sarah J Klausen, Luis A Romero-Soto, Ayesha Liaqat, Zahra Dehghanmanshadi, Knut O Strætkvern, Shaojun Xiong, Carlos Martín","doi":"10.1080/21655979.2025.2536443","DOIUrl":"https://doi.org/10.1080/21655979.2025.2536443","url":null,"abstract":"Spent mushroom substrate (SMS), the main by-product of mushroom cultivation, is a source of sugars that can be released by saccharification. This work aimed at investigating the enzymatic saccharification of the polysaccharides of the SMS of shiitake (Lentinula edodes) and oyster mushroom (Pleurotus ostreatus) and exploring the lignin extraction from the saccharification residues. First, analytical enzymatic saccharification (AES) with a cellulase cocktail and an experimental hemicellulase-rich preparation was applied. AES revealed higher digestibility of both polysaccharides for shiitake SMS than for oyster mushroom SMS. Using the cellulase cocktail, shiitake SMS resulted in a digestibility above 80% and 70% (w/w) for cellulose and xylan, respectively, while the maximum values for oyster mushroom SMS were 52% and 32% (w/w). The experimental enzyme preparation resulted in lower cellulose digestibility and higher xylan digestibility. Still, the saccharification trend between the two SMS types remained unchanged. To enhance the enzymatic saccharification of oyster mushroom SMS, hydrothermal treatment was applied. The treatment improved the enzymatic digestibility of cellulose by up to 84%. A validation experiment at larger scale showed that hydrothermally treated oyster mushroom SMS had a comparable overall conversion with non-treated shiitake SMS. Following a biorefinery strategy, lignin was extracted from the residues of the preparative enzymatic saccharification using the green solvent γ-valerolactone under different temperatures and holding times. The extracted product contained 98.8% lignin and did not contain cellulose or xylan. The results of this study provide the grounds for biorefinery processes enabling recovery of bioactive compounds, fermentable sugars, and high-quality lignin from SMS.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2536443"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the effect of temperature drop on a stable anaerobic fermentation for volatile fatty acids production. 评估温度下降对稳定的厌氧发酵挥发性脂肪酸生产的影响。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-02-03 DOI: 10.1080/21655979.2025.2458369

Manuel Joao Afecto Gonçalves, Cristina González-Fernández, Silvia Greses

{"title":"Assessing the effect of temperature drop on a stable anaerobic fermentation for volatile fatty acids production.","authors":"Manuel Joao Afecto Gonçalves, Cristina González-Fernández, Silvia Greses","doi":"10.1080/21655979.2025.2458369","DOIUrl":"10.1080/21655979.2025.2458369","url":null,"abstract":"Anaerobic fermentation (AF) processes are sensitive to temperature fluctuations, which can influence the microbial activity and overall metabolic performances. Anaerobic reactors can face unforeseen temperature control failures, leading to instabilities in the process. The present study investigated the effect of two short-term temperature perturbations (down to 20°C and 15°C) on AF of food wastes (FWs). While 20°C did not exhibit a negative impact on AF performance maintaining the bioconversion yields over 40%, the reactor subjected to 15°C presented an acidogenic limitation, which decreased the bioconversion yields (36.4 ± 1.8%). As a result, 2.2 ± 0.5 g/L of succinic acid was accumulated in the reactor, being identified as a temperature failure indicator. Once the conditions were reestablished (operation temperature of 25ºC), the metabolic redundancies identified in the reactors allowed the AFs recovery to initial fermentation yields. 20°C was further tested as operational temperature resulting in stable bioconversion yield similar to the Control Reactor (43.2 ± 0.3%). These results showed the feasibility of conducting AF under low temperatures, indicating the potential of this technology to increase the cost-effectiveness of AF at psychrophilic conditions.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2458369"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of Retraction: LncRNA SNHG12 in extracellular vesicles derived from carcinoma-associated fibroblasts promotes cisplatin resistance in non-small cell lung cancer cells. 撤回声明：来自癌相关成纤维细胞的细胞外囊泡中的LncRNA SNHG12促进非小细胞肺癌细胞的顺铂耐药。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-04-23 DOI: 10.1080/21655979.2025.2491957

引用次数: 0

Statement of Retraction: CircSLC7A6 promotes the progression of Wilms' tumor via microRNA-107/ ABL proto-oncogene 2 axis. 撤回声明：CircSLC7A6通过microRNA-107/ ABL原癌基因2轴促进Wilms肿瘤的进展。

IF 4.2 4区生物学

Bioengineered Pub Date : 2025-12-01 Epub Date: 2025-04-23 DOI: 10.1080/21655979.2025.2491941

引用次数: 0