Bioengineered最新文献_第10页

Cutaneotrichosporon curvatum and Yarrowia lipolytica as key players for green chemistry: efficient oil producers from food waste via the carboxylate platform. 曲皮三磷酸体和脂性耶氏菌是绿色化学的关键参与者:通过羧酸盐平台从食物垃圾中高效地生产石油。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1080/21655979.2023.2286723

Elia Tomás-Pejó, Sergio Morales-Palomo, Cristina González-Fernández

{"title":"Cutaneotrichosporon curvatum and Yarrowia lipolytica as key players for green chemistry: efficient oil producers from food waste via the carboxylate platform.","authors":"Elia Tomás-Pejó, Sergio Morales-Palomo, Cristina González-Fernández","doi":"10.1080/21655979.2023.2286723","DOIUrl":"10.1080/21655979.2023.2286723","url":null,"abstract":"Cutaneotrichosporon curvatum and Yarrowia lipolytica can accumulate microbial oils using short-chain fatty acids (SCFA) as carbon sources. SCFAs-rich media often contain significant amounts of nitrogen that prevent high carbon:nitrogen (C:N) ratios necessary to boost lipid production. This work assessed the intrinsic ability of C. curvatum and Y. lipolytica to produce high amounts of microbial oils from these unusual carbon sources. Results demonstrated that minor differences in SCFA concentration (only 2 g/L) had a significant effect on yeast growth and lipid production. A C:N of 80 promoted yeast growth at all SCFA concentrations and favored SCFA consumption at 19 g/L SCFAs. The different SCFA uptake preferences in C. curvatum and Y. lipolytica highlighted the importance of considering the SCFA profile to select a suitable yeast strain for microbial oils production. At the most challenging SCFA concentration (19 g/L), 57.2% ±1.6% (w/w) and 78.4 ± 0.6% (w/w) lipid content were obtained in C. curvatum and Y. lipolytica, respectively. These values are among the highest reported for wild-type strains. To circumvent the challenges associated with media with high nitrogen content, this report also proved struvite precipitation as an effective method for increasing lipid production (from 17.9 ± 3.9% (w/w) to 41.9 ± 2.6% (w/w)) after nitrogen removal in food waste-derived media.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of Retraction: The long non-coding RNA ASMTL-AS1 promotes hepatocellular carcinoma progression by sponging miR-1343-3p that suppresses LAMC1 (laminin subunit gamma 1). 撤回声明：长非编码RNA ASMTL-AS1通过吸收抑制LAMC1（层粘连蛋白亚单位γ1）的miR-1343-3p来促进肝细胞癌的进展。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269349

引用次数: 0

Statement of Retraction: Circular RNA protein tyrosine kinase 2 (circPTK2) promotes colorectal cancer proliferation, migration, invasion and chemoresistance. 撤回声明：环状RNA蛋白酪氨酸激酶2（circPTK2）促进癌症增殖、迁移、侵袭和化疗耐药性。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269352

引用次数: 0

Modified polymer membranes for the removal of pharmaceutical active compounds in wastewater and its mechanism-A review. 改性聚合物膜去除废水中药物活性化合物及其机理研究进展。

IF 4.2 4区生物学

Bioengineered Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2252234

Anisa Ratnasari

{"title":"Modified polymer membranes for the removal of pharmaceutical active compounds in wastewater and its mechanism-A review.","authors":"Anisa Ratnasari","doi":"10.1080/21655979.2023.2252234","DOIUrl":"10.1080/21655979.2023.2252234","url":null,"abstract":"Membrane technology can play a suitable role in removing pharmaceutical active compounds since it requires low energy and simple operation. Even though membrane technology has progressed for wastewater applications nowadays, modifying membranes to achieve the strong desired membrane performance is still needed. Thus, this study overviews a comprehensive insight into the application of modified polymer membranes to remove pharmaceutical active compounds from wastewater. Biotoxicity of pharmaceutical active compounds is first prescribed to gain deep insight into how membranes can remove pharmaceutical active compounds from wastewater. Then, the behavior of the diffusion mechanism can be concisely determined using mass transfer factor model that represented by β and B with value up to 2.004 g h mg-1 and 1.833 mg g-1 for organic compounds including pharmaceutical active compounds. The model refers to the adsorption of solute to attach onto acceptor sites of the membrane surface, external mass transport of solute materials from the bulk liquid to the membrane surface, and internal mass transfer to diffuse a solute toward acceptor sites of the membrane surface with evidenced up to 0.999. Different pharmaceutical compounds have different solubility and relates to the membrane hydrophilicity properties and mechanisms. Ultimately, challenges and future recommendations have been presented to view the future need to enhance membrane performance regarding fouling mitigation and recovering compounds. Afterwards, the discussion of this study is projected to play a critical role in advance of better-quality membrane technologies for removing pharmaceutical active compounds from wastewater in an eco-friendly strategy and without damaging the ecosystem.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/f6/KBIE_14_2252234.PMC10506444.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10308170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in microbial engineering approaches for wastewater treatment: a review. 废水处理微生物工程方法的最新进展：综述。

IF 4.2 4区生物学

Bioengineered Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2184518

Monika Sharma, Sangita Agarwal, Richa Agarwal Malik, Gaurav Kumar, Dan Bahadur Pal, Mamun Mandal, Abhijit Sarkar, Farkad Bantun, Shafiul Haque, Pardeep Singh, Neha Srivastava, Vijai Kumar Gupta

{"title":"Recent advances in microbial engineering approaches for wastewater treatment: a review.","authors":"Monika Sharma, Sangita Agarwal, Richa Agarwal Malik, Gaurav Kumar, Dan Bahadur Pal, Mamun Mandal, Abhijit Sarkar, Farkad Bantun, Shafiul Haque, Pardeep Singh, Neha Srivastava, Vijai Kumar Gupta","doi":"10.1080/21655979.2023.2184518","DOIUrl":"10.1080/21655979.2023.2184518","url":null,"abstract":"In the present era of global climate change, the scarcity of potable water is increasing both due to natural and anthropogenic causes. Water is the elixir of life, and its usage has risen significantly due to escalating economic activities, widespread urbanization, and industrialization. The increasing water scarcity and rising contamination have compelled, scientists and researchers, to adopt feasible and sustainable wastewater treatment methods in meeting the growing demand for freshwater. Presently, various waste treatment technologies are adopted across the globe, such as physical, chemical, and biological treatment processes. There is a need to replace these technologies with sustainable and green technology that encourages the use of microorganisms since they have proven to be more effective in water treatment processes. The present review article is focused on demonstrating how effectively various microbes can be used in wastewater treatment to achieve environmental sustainability and economic feasibility. The microbial consortium used for water treatment offers many advantages over pure culture. There is an urgent need to develop hybrid treatment technology for the effective remediation of various organic and inorganic pollutants from wastewater.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9892894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia. 用于治疗单核细胞白血病的植物和植物细胞表达的重组免疫毒素的设计、优化、生产和活性测试。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2244235

Matthias Knödler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel

{"title":"Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia.","authors":"Matthias Knödler, Katharina Frank, Lucy Kerpen, Johannes Felix Buyel","doi":"10.1080/21655979.2023.2244235","DOIUrl":"10.1080/21655979.2023.2244235","url":null,"abstract":"Antibody-drug conjugates (ADCs) can improve therapeutic indices compared to plain monoclonal antibodies (mAbs). However, ADC synthesis is complex because the components are produced separately in CHO cells (mAb) and often by chemical synthesis (drug). They are individually purified, coupled, and then the ADC is purified, increasing production costs compared to regular mAbs. In contrast, it is easier to produce recombinant fusion proteins consisting of an antibody derivative, linker and proteinaceous toxin, i.e. a recombinant immunotoxin (RIT). Plants are capable of the post-translational modifications needed for functional antibodies and can also express active protein toxins such as the recombinant mistletoe lectin viscumin, which is not possible in prokaryotes and mammalian cells respectively. Here, we used Nicotiana benthamiana and N. tabacum plants as well as tobacco BY-2 cell-based plant cell packs (PCPs) to produce effective RITs targeting CD64 as required for the treatment of myelomonocytic leukemia. We compared RITs with different subcellular targeting signals, linkers, and proteinaceous toxins. The accumulation of selected candidates was improved to ~ 40 mg kg-1 wet biomass using a design of experiments approach, and corresponding proteins were isolated with a purity of ~ 80% using an optimized affinity chromatography method with an overall yield of ~ 84%. One anti-CD64 targeted viscumin-based drug candidate was characterized in terms of storage stability and cytotoxicity test in vitro using human myelomonocytic leukemia cell lines. We identified bottlenecks in the plant-based expression platform that require further improvement and assessed critical process parameters that should be considered during process development for plant-made RITs.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/75/KBIE_14_2244235.PMC10444015.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10413342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Databases for biomass and waste biorefinery - a mini-review and SWOT analysis. 生物质和废物生物炼制数据库-一个小型回顾和SWOT分析。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-11-29 DOI: 10.1080/21655979.2023.2286722

Morgen Mukamwi, Tosin Somorin, Raimonda Soloha, Elina Dace

{"title":"Databases for biomass and waste biorefinery - a mini-review and SWOT analysis.","authors":"Morgen Mukamwi, Tosin Somorin, Raimonda Soloha, Elina Dace","doi":"10.1080/21655979.2023.2286722","DOIUrl":"10.1080/21655979.2023.2286722","url":null,"abstract":"The world is facing problems of the increasing amount of resources wasted as the world population grows. Biowaste streams form a significant part of the overall waste generation, and a circular economy utilizing this biowaste will significantly reduce waste whilst lowering the anthropogenic carbon footprint. Due to their energy content and high concentration of hydrocarbon molecules, bio-based waste streams have the potential to be transformed into valorized products (energy, fuels, and chemicals) using biorefinery technologies. In this work, a mini-review has been conducted on available, mostly European databases on existing biomass types and biorefinery technologies to provide a framework for a desirable, comprehensive database connecting bio-based waste streams, biorefinery technologies and bioproducts, as well as the geographical distribution of feedstocks and biorefineries. The database assessment utilized the SWOT (strengths, weakness, opportunities, threats) methodology to support benchmark analysis and to identify critical gaps in underlying data structures that could be included in a single database. The results show that current databases are useful but insufficient for waste biorefineries due to limited quality and quantity as well as the usability of data. A comprehensive database or improved database cluster would be necessary, not only for technology development but for better investment and policy decisions. The development of the new database architecture would need to incorporate the aspects: expansion of database scope and content depth, improved usability, accessibility, applicability, update frequency, openness to new contributions, process descriptions and parameters, and technology readiness level.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of exogenous polyamines on the secondary somatic embryogenesis of cork oak (Quercus suber L.). 外源多胺对栓皮栎次生体细胞胚胎发生的影响。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-11-29 DOI: 10.1080/21655979.2023.2288354

Naouar Ben Ali, Rajae Benkaddour, Safaa Rahmouni, Ouafaa Hamdoun, Ibtissam Boussaoudi, Mustapha Hassoun, Latifa Azaroual, Alain Badoc, Patrick Martin, Ahmed Lamarti

{"title":"Influence of exogenous polyamines on the secondary somatic embryogenesis of cork oak (Quercus suber L.).","authors":"Naouar Ben Ali, Rajae Benkaddour, Safaa Rahmouni, Ouafaa Hamdoun, Ibtissam Boussaoudi, Mustapha Hassoun, Latifa Azaroual, Alain Badoc, Patrick Martin, Ahmed Lamarti","doi":"10.1080/21655979.2023.2288354","DOIUrl":"10.1080/21655979.2023.2288354","url":null,"abstract":"Quercus suber L. is the main woody tree species in the Mediterranean basin. The in vitro regeneration from adult material, through primary somatic embryogenesis, is a well-known process, but the use of secondary somatic embryos for plant regeneration remains a very sparsely studied process. The main objective of this work is to explore the cork oak regeneration potential by using the secondary somatic embryogenesis process. Mainly, in this work, we report the polyamine effect. Explants used consisted on primary mature embryos, derived from leaves rejuvenated by epicormic shoot of the Moroccan Quercus suber. Three different polyamines were added to the basal medium, which was composed by macronutrients of N30K, 30 g/l glucose, and 7 g/l agar. Three polyamines, Putrescine, Spermine, and Spermidine, were added to the basal medium at 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l. Explants were tested after 8 weeks. Morphological analysis showed that the medium with 0.4 mg/l Spermidine provided the best result for secondary embryos, which corresponds to a very significant (p < 0.05) increase of 375%. The number of secondary embryos directly formed was 2.70 ± 0.51. Similarly, the optimum concentrations for high number of clusters (0.50 ± 0.11) and embryo clusters (1.43 ± 0.35) were increased by 145% and 158%. The addition of the polyamine also acted on the quality of embryos formed. A very significant (p < 0.05) increase in the size of secondary embryos was observed compared to the medium without polyamines. Spermidine showed the greatest increase (about 38%).","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138457653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell. 利用微生物燃料电池从藻类中生产新的构建块和生物能源的可持续循环生物精炼方法。

IF 4.2 4区生物学

Bioengineered Pub Date : 2023-12-01 DOI: 10.1080/21655979.2023.2236842

Kevin Tian Xiang Tong, Inn Shi Tan, Henry Chee Yew Foo, Pau Loke Show, Man Kee Lam, Mee Kee Wong

{"title":"Sustainable circular biorefinery approach for novel building blocks and bioenergy production from algae using microbial fuel cell.","authors":"Kevin Tian Xiang Tong, Inn Shi Tan, Henry Chee Yew Foo, Pau Loke Show, Man Kee Lam, Mee Kee Wong","doi":"10.1080/21655979.2023.2236842","DOIUrl":"10.1080/21655979.2023.2236842","url":null,"abstract":"The imminent need for transition to a circular biorefinery using microbial fuel cells (MFC), based on the valorization of renewable resources, will ameliorate the carbon footprint induced by industrialization. MFC catalyzed by bioelectrochemical process drew significant attention initially for its exceptional potential for integrated production of biochemicals and bioenergy. Nonetheless, the associated costly bioproduct production and slow microbial kinetics have constrained its commercialization. This review encompasses the potential and development of macroalgal biomass as a substrate in the MFC system for L-lactic acid (L-LA) and bioelectricity generation. Besides, an insight into the state-of-the-art technological advancement in the MFC system is also deliberated in detail. Investigations in recent years have shown that MFC developed with different anolyte enhances power density from several µW/m2 up to 8160 mW/m2. Further, this review provides a plausible picture of macroalgal-based L-LA and bioelectricity circular biorefinery in the MFC system for future research directions.","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9871760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statement of Retraction: N(6)-adenosine-methyltransferase-14 promotes glioma tumorigenesis by repressing argininosuccinate synthase 1 expression in an m6A-dependent manner. 撤回声明：N（6）-腺苷-甲基转移酶-14通过以m6A依赖的方式抑制精氨酸琥珀酸合酶1的表达，促进神经胶质瘤的发生。

IF 4.9 4区生物学

Bioengineered Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1080/21655979.2023.2269351

引用次数: 0