International journal of molecular imaging最新文献

{"title":"Functional Graphene-Based Nanobioimaging Platforms: New Powered Real-Time Interfaces","authors":"F. Menaa","doi":"10.4172/2155-9937.1000E103","DOIUrl":"https://doi.org/10.4172/2155-9937.1000E103","url":null,"abstract":"Copyright: © 2013 Menaa F. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In the recent years, graphene (G)-based nanomaterials (e.g. graphene oxide (GO), G hybrid nanocomposites) are increasingly explored for real-time imaging of biomolecules or cells [1,2].","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"196 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2013-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77412400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of the Detection of Amino Acids in Tumor Tissue with 1H Magnetic Resonance Spectroscopy in vivo","authors":"D. Bartusik, D. Aebisher, B. Tomanek","doi":"10.4172/2155-9937.1000112","DOIUrl":"https://doi.org/10.4172/2155-9937.1000112","url":null,"abstract":"The biochemical components of tumor tissue are generally different from normal tissue. A variety of techniques, including of magnetic resonance (MR) are used to study metabolic compounds and their associated biochemical pathways in tumor tissue in vivo. This review provides an overview of applications of MR spectroscopy (MRS) to the identification of biochemical characteristics, e.g. amino acids present during tumor growth. MRS is a technology that allows spatial localization and structural studies of amino acids and is gaining in use as a diagnostic tool. This review is intended to provide information about potential applications of MRS to non-invasive probing of the underlying amino acid biochemistry in tumors. Articles discussed in this review are classified into the following categories:hardware for 1H MRS, acquisition methods, software, and 1H MRS of amino acids in tumor tissue in vivo.","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"9 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88261944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Beacon-Based Microrna Biosensor for Imaging EPC-Treated Cellular Therapy of Ischemia","authors":"Chang Hyun Lee, J. Chae, H. Ko, Soonhag Kim","doi":"10.4172/2155-9937.1000113","DOIUrl":"https://doi.org/10.4172/2155-9937.1000113","url":null,"abstract":"Angiogenesis, the process of new blood vessel formation, is an important therapeutic target in cardiovascular and malignant diseases for diverse reasons. Molecular imaging for angiogenesis has been attracted due to the use of anti-angiogenic therapeutic drugs to treat tumours, and of therapeutic angiogenesis induction to treat vascular diseases. We developed a novel biosensor of imaging microRNA126 (mir126) expressed during angiogenesis using a miRNA Molecular Beacon (MB) composed of a stem loop-structured DNA complementary to mir126 and Cy5.5 (near infrared, NIR)-black hole quencher 2 (BHQ2) (mir126 NIR MB). Mir126 in cord blood-derived endothelial precursor cells (CB-EPCs) was highly expressed and more expressed after the wound healing. The quantitative and qualitative fluorescence intensity of the mir126 NIR MB was high in CB-EPC and significantly increased after the wound healing, showing a great specificity of sensing endogenous mir126. From the CB-EPC-treated hind limb ischemia, cellular morphology and immune histochemical analysis using antibodies of vWF and CD31 showed a successful induction of angiogenesis and vascularisation and fluorescence signals of the mir126 NIR MB was gradually increased during 6 days of the cellular therapy and much stronger than the signals of laser Doppler imaging. The mir126 NIR MB demonstrated that the mir126sensor will be useful for early diagnosis of cellular therapy of ischemia and non-invasively sensitive imaging for cellular developments, diagnosis of disease and cellular therapy related to the miRNA function.","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"67 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78157670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PET-CT Imaging in Breast Cancer Patients: New Tracers, Future Directions","authors":"Tímea TÅkés","doi":"10.4172/2155-9937.1000111","DOIUrl":"https://doi.org/10.4172/2155-9937.1000111","url":null,"abstract":"Positron Emission Tomography using 18F-fluoro-deoxi-glucose (FDG-PET) is giving a new perspective in disease staging and therapeutic response evaluation in daily oncological practice. To understand better the biological behavior and therapeutic response of breast cancer, new tracers and targets of molecular imaging are under investigation.These tracers may lead to non-invasive evaluation of the main, leading therapeutic decision-maker properties of metastatic breast cancer such as receptor status, proliferation activity and therapy resistance, and could also become predictive markers in the early measurement of therapeutic response in the neoadjuvant treatment of locally advanced breast cancer. However, these new agents are currently not available in the daily practice; the preliminary results are promising.","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"7 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84285845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Biological Evaluation of the 99mtcn-Gemifloxacin Dithiocarbamate Complex: A Novel Streptococcus Pneumoniae Infection Imaging Agent","authors":"S. Q. Shah, Muhammad Rafi Ullah Khan","doi":"10.4172/2155-9937.1000110","DOIUrl":"https://doi.org/10.4172/2155-9937.1000110","url":null,"abstract":"Synthesis and biological evaluation of the 99mTcN-Gemifloxacin dithiocarbamate (99mTcN-GIND) complex was investigated in terms of radiochemical stability (RCP) in saline, serum, in-vitro binding with Streptococcus pneumoniae (S. pneumoniae) and biodistribution in male Wistar rats artificially infected with living and heat killed S. pneumoniae. The maximum RCP was 98.25 ± 0.30% at 30 min and decreased to 91.25 ± 0.34% within 240 min. The complex showed stable behavior (in-vitro) in serum at 37°C with a 14.35% undesirable side products within 16 h. The complex showed 71.25% in-vitro binding S. pneumoniae. The uptake of the complex in the infected muscle was six times higher than the inflamed and normal muscles of the MWR infected with living S. pneumoniae. The promising (in-vitro and in-vivo) radiochemical and biological behavior posed the 99mTcN-GIND complex as a potential radiotracer for S. pneumoniae infection.","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"2 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88231621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility and merits of performing preclinical imaging on clinical radiology and nuclear medicine systems.","authors":"Mehmet Bilgen","doi":"10.1155/2013/923823","DOIUrl":"https://doi.org/10.1155/2013/923823","url":null,"abstract":"<p><p>Aim. Researchers have limited access to systems dedicated to imaging small laboratory animals. This paper aims to investigate the feasibility and merits of performing preclinical imaging on clinical systems. Materials and Methods. Scans were performed on rat and mouse models of diseases or injuries on four radiology systems, tomosynthesis, computed tomography (CT), positron emission tomography/computed tomography (PET-CT), and Magnetic Resonance Imaging (MRI), based on the availability at the author's institute. Results. Tomosysthesis delineated soft tissue anatomy and hard tissue structure with superb contrast and spatial resolution at minimal scan time and effort. CT allowed high resolution volumetric visualization of bones. Molecular imaging with PET was useful for detecting cancerous tissue in mouse but at the expense of poor resolution. MRI depicted abnormal or intervened tissue at quality and resolution sufficient for experimental studies. The paper discussed limitations of the clinical systems in preclinical imaging as well as challenges regarding the need of additional gadgets, modifications, or upgrades required for longitudinally scanning animals under anesthesia while monitoring their vital signs. Conclusion. Clinical imaging technologies can potentially make cost-effective and efficient contributions to preclinical efforts in obtaining anatomical, structural, and functional information from the underlying tissue while minimally compromising the data quality in certain situations. </p>","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"2013 ","pages":"923823"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/923823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32084967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ROI Placement on PET-Based Assessment of Tumor Response to Therapy.","authors":"Mike Sattarivand,&nbsp;Curtis Caldwell,&nbsp;Ian Poon,&nbsp;Hany Soliman,&nbsp;Katherine Mah","doi":"10.1155/2013/132804","DOIUrl":"https://doi.org/10.1155/2013/132804","url":null,"abstract":"<p><p>Purpose. Quantitative PET response assessment during therapy requires regions of interest (ROI). Commonly, a fixed-size ROI is placed at the maximum uptake point in the pretreatment study. For intratreatment, the ROI is placed either at the maximum uptake point (ROIpeak) or at the same location as the pretreatment ROI (ROIsame). We have evaluated the effects of the ROI placement on response assessment. Methods. PET scans of 15 head and neck cancer patients were used to evaluate the effects of the two ROI methods on response assessment. Results. The average intratreatment ROIpeak uptake was 13.4% higher than the ROIsame uptake (range -14% to 38%). The average relative change in ROIpeak uptake was 7.9% lower than ROIsame uptake (range -5% to 36%), resulting in ambiguous tumour classification in 19% of the tumours. Conclusion. Quantitative PET response assessment using a fixed-size ROI is sensitive the ROI placement. The difference between ROIpeak and ROIsame could be substantial resulting in ambiguous response assessment. Although the fixed-size ROI is simple to implement, it is also prone to the limitations and should be used with caution. Clinical trial data are necessary to establish reliable thresholds for fixed-size ROI techniques and to evaluate their efficacy for response assessment.</p>","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":" ","pages":"132804"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/132804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40230003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo tracking of murine adipose tissue-derived multipotent adult stem cells and ex vivo cross-validation.","authors":"Chiara Garrovo,&nbsp;Natascha Bergamin,&nbsp;Dave Bates,&nbsp;Daniela Cesselli,&nbsp;Antonio Paolo Beltrami,&nbsp;Andrea Lorenzon,&nbsp;Roberto Ferrari,&nbsp;Carlo Alberto Beltrami,&nbsp;Vito Lorusso,&nbsp;Stefania Biffi","doi":"10.1155/2013/426961","DOIUrl":"https://doi.org/10.1155/2013/426961","url":null,"abstract":"<p><p>Stem cells are characterized by the ability to renew themselves and to differentiate into specialized cell types, while stem cell therapy is believed to treat a number of different human diseases through either cell regeneration or paracrine effects. Herein, an in vivo and ex vivo near infrared time domain (NIR TD) optical imaging study was undertaken to evaluate the migratory ability of murine adipose tissue-derived multipotent adult stem cells [mAT-MASC] after intramuscular injection in mice. In vivo NIR TD optical imaging data analysis showed a migration of DiD-labelled mAT-MASC in the leg opposite the injection site, which was confirmed by a fibered confocal microendoscopy system. Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells. Considering a potential microenvironmental contamination, a cross-validation study by multimodality approaches was followed: mAT-MASC were isolated from male mice expressing constitutively eGFP, which was detectable using techniques of immunofluorescence and qPCR. Y-chromosome positive cells, injected into wild-type female recipients, were detected by FISH. Cross-validation confirmed the data obtained by in vivo/ex vivo TD optical imaging analysis. In summary, our data demonstrates the usefulness of NIR TD optical imaging in tracking delivered cells, giving insights into the migratory properties of the injected cells.</p>","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"2013 ","pages":"426961"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/426961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31232416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Clinical-Grade [(18)F]-Fluoroestradiol as a Surrogate PET Biomarker for the Evaluation of Estrogen Receptor-Targeting Therapeutic Drug.","authors":"Manish Dixit, Jianfeng Shi, Ling Wei, George Afari, Sibaprasad Bhattacharyya","doi":"10.1155/2013/278607","DOIUrl":"10.1155/2013/278607","url":null,"abstract":"<p><p>16 α -[(18)F]-fluoroestradiol ([(18)F]FES), a steroid-based positron emission tomography (PET) tracer, has emerged as a dependable tracer for the evaluation and management of estrogen receptor-positive (ER+) breast cancer patients. We have developed a fully automatic, one-pot procedure for the synthesis of [(18)F]FES using the Eckert & Ziegler (E & Z) radiomodular system. After [(18)F]fluorination, the intermediate was hydrolyzed with 2.0 M HCl twice and neutralized with sodium bicarbonate. After high-performance liquid chromatography (HPLC) purification, the decay-corrected radiochemical yield and purity of [(18)F]FES were 40 ± 5.0% (n = 12) and >97%, respectively. The product was stable up to 10 h. Total synthesis time including HPLC purification was 80 min. This new, fully automated rapid synthetic procedure provided high and reproducible yields of [(18)F]FES. Quality control (QC) tests showed that the [(18)F]FES produced by this method met all specifications for human injection.</p>","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"2013 ","pages":"278607"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Negative Matrix Factorization Based Input Function Extraction for Mouse Imaging in Small Animal PET - Comparison with Arterial Blood Sampling and Factor Analysis","authors":"Dominik Schulz, A. Tapfer, A. Buck, S. Reeder, M. Miederer, E. Weidl, S. Ziegler, M. Schwaiger, R. Bundschuh","doi":"10.4172/2155-9937.1000108","DOIUrl":"https://doi.org/10.4172/2155-9937.1000108","url":null,"abstract":"Objectives: Retrieving the accurate time-tracer activity concentration curve of the blood (arterial input function) is mandatory for performing bio kinetic model analysis of dynamic PET data. Especially in small rodents, gathering the input function remains an active area of research as no generally applicable solution was found so far. While surgically catheterizing blood vessels of rodents is possible, it is labour intensive and time resolution of blood sampling is restricted due to the limited amount of overall blood and the procedure of blood withdrawal itself. Obtaining the input function from the PET images themselves seems thus to be favourable, but suffers from several factors, one of them being spill-in of adjacent tissues. Particularly in mice and for [18F]FDG, the spill-in complicates using the time-activity curve (TAC) from a region of interest (ROI) over the left ventricle (LV) because the signal of the ROI contains contributions from both, myocardial uptake as well as arterial blood activity. We propose non-negative matrix factorization (NMF) as an image based algorithm for separating myocardial tracer concentration from the blood input function. The aim of this study was to evaluate the potential of NMF as an image based algorithm for retrieving the input function by comparison with blood sampling and Factor Analysis (FA). Method: The femoral arteries of eight mice were surgically catheterized. With the injection of [18F]FDG, a 60 minute PET scan was started during which arterial blood samples were manually drawn from the catheter. For analysis, NMF and FA were performed in a ROI placed over the LV. The NMF algorithm shares similarities with principal component analysis and FA, the advantage over the later two being its non-negativity constraint. For normalization of the NMF extracted curve, the peak value of tracer activity in an early image of the LV and a late blood sample was used. The normalized NMF curve was visually compared to the TAC retrieved from the blood samples and to the FA retrieved TAC. For a quantitative comparison of performance, Pearson correlation and square-root of sum of squares (RSS) between NMF/FA and blood sampling curves was calculated. Results: TAC based on NMF, FA and arterial blood samples were obtained and compared in all 8 mice. The NMF derived curves described the blood sampling based curves visually significantly better than FA. Pearson correlation between NMF and blood sampling curves ranged from 0.21 to 0.92 with an average of 0.69. Pearson correlation for FA ranged from 0.46 to 0.81 with an average of 0.65. Mean RSS was 2.70E + 006 for NMF and 3.40E + 006 for FA. Conclusion: In the examined parameters, visual accordance, Pearson correlation and RSS, NMF performs superior to FA and seems to be a promising method for the extraction of the input function from PET images of small rodents without the need for arterial blood sampling.","PeriodicalId":89157,"journal":{"name":"International journal of molecular imaging","volume":"46 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83243752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}