Biogerontology最新文献_第7页

Combined dasatinib and quercetin treatment contributes to skin rejuvenation through selective elimination of senescent cells in vitro and in vivo 达沙替尼和槲皮素联合疗法通过在体外和体内选择性消除衰老细胞，促进皮肤年轻化

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-15 DOI: 10.1007/s10522-024-10103-z

Kento Takaya, Kazuo Kishi

{"title":"Combined dasatinib and quercetin treatment contributes to skin rejuvenation through selective elimination of senescent cells in vitro and in vivo","authors":"Kento Takaya, Kazuo Kishi","doi":"10.1007/s10522-024-10103-z","DOIUrl":"https://doi.org/10.1007/s10522-024-10103-z","url":null,"abstract":"The skin’s protective functions are compromised over time by both endogenous and exogenous aging. Senescence is well-documented in skin phenotypes, such as wrinkling and sagging, a consequence of the senescence-associated secretory phenotype (SASP) that involves the accumulation of senescent fibroblasts, chronic inflammation, and collagen remodeling. Although therapeutic approaches for eliminating senescent cells from the skin are available, their efficacy remains unclear. Accordingly, we aimed to examine the effects of dasatinib in combination with quercetin (D + Q) on senescent human skin fibroblasts and aging human skin. Senescence was induced in human dermal fibroblasts (HDFs) using approaches such as long-term passaging, ionizing radiation, and doxorubicin treatment. The generated senescent cells were treated with D + Q or vehicle. Additionally, a mouse-human chimera model was generated by subcutaneously transplanting whole-skin grafts of aged individuals onto nude mice. Mouse models were administered D + Q or vehicle by oral gavage for 30 days. Subsequently, skin samples were harvested and stained for senescence-associated beta-galactosidase. Senescence-associated markers were assessed by western blotting, reverse transcription-quantitative PCR and histological analyses. Herein, D + Q selectively eliminated senescent HDFs in all cellular models of induced senescence. Additionally, D + Q-treated aged human skin grafts exhibited increased collagen density and suppression of the SASP compared with control grafts. No adverse events were observed during the study period. Collectively, D + Q could ameliorate skin aging through selective elimination of senescent dermal fibroblasts and suppression of the SASP. Our findings suggest that D + Q could be developed as an effective therapeutic approach for combating skin aging.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"21 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140591061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-sequencing exploration on SIR2 and SOD genes in Polyalthia longifolia leaf methanolic extracts (PLME) mediated anti-aging effects in Saccharomyces cerevisiae BY611 yeast cells RNA 序列分析探究长叶聚伞花科植物叶甲醇提取物（PLME）介导的 BY611 酵母菌细胞抗衰老效应中的 SIR2 和 SOD 基因

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-15 DOI: 10.1007/s10522-024-10104-y

Manisekaran Hemagirri, Yeng Chen, Subash C. B. Gopinath, Mohd Adnan, Mitesh Patel, Sreenivasan Sasidharan

{"title":"RNA-sequencing exploration on SIR2 and SOD genes in Polyalthia longifolia leaf methanolic extracts (PLME) mediated anti-aging effects in Saccharomyces cerevisiae BY611 yeast cells","authors":"Manisekaran Hemagirri, Yeng Chen, Subash C. B. Gopinath, Mohd Adnan, Mitesh Patel, Sreenivasan Sasidharan","doi":"10.1007/s10522-024-10104-y","DOIUrl":"https://doi.org/10.1007/s10522-024-10104-y","url":null,"abstract":"Polyalthia longifolia is well-known for its abundance of polyphenol content and traditional medicinal uses. Previous research has demonstrated that the methanolic extract of P. longifolia leaves (PLME, 1 mg/mL) possesses anti-aging properties in Saccharomyces cerevisiae BY611 yeast cells. Building on these findings, this study delves deeper into the potential antiaging mechanism of PLME, by analyzing the transcriptional responses of BY611 cells treated with PLME using RNA-sequencing (RNA-seq) technology. The RNA-seq analysis results identified 1691 significantly (padj < 0.05) differentially expressed genes, with 947 upregulated and 744 downregulated genes. Notably, the expression of three important aging-related genes, SIR2, SOD1, and SOD2, showed a significant difference following PLME treatment. The subsequent integration of these targeted genes with GO and KEGG pathway analysis revealed the multifaceted nature of PLME’s anti-aging effects in BY611 yeast cells. Enriched GO and KEGG analysis showed that PLME treatment promotes the upregulation of SIR2, SOD1, and SOD2 genes, leading to a boosted cellular antioxidant defense system, reduced oxidative stress, regulated cell metabolism, and maintain genome stability. These collectively increased longevities in PLME-treated BY611 yeast cells and indicate the potential anti-aging action of PLME through the modulation of SIR2 and SOD genes. The present study provided novel insights into the roles of SIR2, SOD1, and SOD2 genes in the anti-aging effects of PLME treatment, offering promising interventions for promoting healthy aging.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"38 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140591069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resveratrol activates autophagy and protects from UVA-induced photoaging in human skin fibroblasts and the skin of male mice by regulating the AMPK pathway 白藜芦醇通过调节 AMPK 通路激活人类皮肤成纤维细胞和雄性小鼠皮肤的自噬功能，并防止 UVA 引起的光老化

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-09 DOI: 10.1007/s10522-024-10099-6

Yangmin Xia, Hao Zhang, Xiangyi Wu, Ye Xu, Qian Tan

{"title":"Resveratrol activates autophagy and protects from UVA-induced photoaging in human skin fibroblasts and the skin of male mice by regulating the AMPK pathway","authors":"Yangmin Xia, Hao Zhang, Xiangyi Wu, Ye Xu, Qian Tan","doi":"10.1007/s10522-024-10099-6","DOIUrl":"https://doi.org/10.1007/s10522-024-10099-6","url":null,"abstract":"Skin photoaging is mostly caused by ultraviolet A (UVA), although active medications to effectively counteract UVA-induced photoaging have not yet been created. Resveratrol, a naturally occurring polyphenol found in the skin of grapes, has been shown to have various biological functions such as anti-inflammatory and antioxidant characteristics. However, the role of resveratrol in UVA-induced photoaging has not been clarified. We investigated the mechanism of action of resveratrol by UVA irradiation of human skin fibroblasts (HSF) and innovatively modified a mouse model of photoaging. The results demonstrated that resveratrol promoted AMP-activated protein kinase (AMPK) phosphorylation to activate autophagy, reduce reactive oxygen species (ROS) production, inhibit apoptosis, and restore normal cell cycle to alleviate UVA-induced photoaging. In addition, subcutaneous injection of resveratrol not only improved the symptoms of roughness, erythema, and increased wrinkles in the skin of UVA photodamaged mice, but also alleviated epidermal hyperkeratosis and hyperpigmentation, reduced inflammatory responses, and inhibited collagen fiber degradation. In conclusion, our studies proved that resveratrol can treat UVA-induced photoaging and elucidated the possible molecular mechanisms involved, providing a new therapeutic strategy for future anti-aging.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"215 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140591488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic trans fatty acid consumption shortens lifespan in male Drosophila melanogaster on a high-sugar and high-fat diet 长期摄入反式脂肪酸会缩短高糖高脂饮食雄性黑腹果蝇的寿命

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-06 DOI: 10.1007/s10522-024-10101-1

{"title":"Chronic trans fatty acid consumption shortens lifespan in male Drosophila melanogaster on a high-sugar and high-fat diet","authors":"","doi":"10.1007/s10522-024-10101-1","DOIUrl":"https://doi.org/10.1007/s10522-024-10101-1","url":null,"abstract":"<h3>Abstract</h3> Aging entails the progressive decline in the body’s self-regulation and functionality over time. Notably, obesity and aging exhibit parallel phenotypes, with obesity further accelerating the aging process across multiple dimensions and diminishing lifespan. In this study, we explored the impact of trans fatty acid (TFA) consumption on the overall health and lifespan of male Drosophila melanogaster under an isocaloric high-sugar and high-fat diet. Our results indicate that TFA intake results in a shortened lifespan, elevated body weight, and increased triglyceride levels in flies fed a high-sugar and high-fat diet with equivalent caloric intake. Additionally, TFA exposure induces oxidative stress, locomotor deficits, and damage to the intestinal barrier in flies. Collectively, chronic TFA consumption expedites the aging process and reduces the lifespan of male Drosophila melanogaster. These results contribute supplementary evidence regarding the adverse health effects associated with TFAs. <h3>Graphical Abstract</h3> Trans fatty acid intake results in a shortened lifespan, elevated body weight, and increased triglyceride levels in male Drosophila melanogaster fed a high-sugar and high-fat diet with equivalent caloric intake. Additionally, Trans fatty acid exposure induces oxidative stress, locomotor deficits, and damage to the intestinal barrier in flies. <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/10522_2024_10101_Figa_HTML.png\"/> ","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"1 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T-cell lymphocytes' aging clock: telomeres, telomerase and aging. T细胞淋巴细胞的衰老时钟：端粒、端粒酶和衰老。

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-01 Epub Date: 2023-11-02 DOI: 10.1007/s10522-023-10075-6

Alain Chebly, Charbel Khalil, Alexandra Kuzyk, Marie Beylot-Barry, Edith Chevret

引用次数: 0

Telomere shortening induces aging-associated phenotypes in hiPSC-derived neurons and astrocytes. 端粒缩短在hipsc来源的神经元和星形胶质细胞中诱导衰老相关表型。

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-01 Epub Date: 2023-11-21 DOI: 10.1007/s10522-023-10076-5

Jasmine Harley, Munirah Mohamad Santosa, Chong Yi Ng, Oleg V Grinchuk, Jin-Hui Hor, Yajing Liang, Valerie Jingwen Lim, Wee Wei Tee, Derrick Sek Tong Ong, Shi-Yan Ng

{"title":"Telomere shortening induces aging-associated phenotypes in hiPSC-derived neurons and astrocytes.","authors":"Jasmine Harley, Munirah Mohamad Santosa, Chong Yi Ng, Oleg V Grinchuk, Jin-Hui Hor, Yajing Liang, Valerie Jingwen Lim, Wee Wei Tee, Derrick Sek Tong Ong, Shi-Yan Ng","doi":"10.1007/s10522-023-10076-5","DOIUrl":"10.1007/s10522-023-10076-5","url":null,"abstract":"Telomere shortening is a well-established hallmark of cellular aging. Telomerase reverse transcriptase (TERT) plays a crucial role in maintaining the length of telomeres, which are specialised protective caps at the end of chromosomes. The lack of in vitro aging models, particularly for the central nervous system (CNS), has impeded progress in understanding aging and age-associated neurodegenerative diseases. In this study, we aimed to explore the possibility of inducing aging-associated features in cell types of the CNS using hiPSC (human induced pluripotent stem cell) technology. To achieve this, we utilised CRISPR/Cas9 to generate hiPSCs with a loss of telomerase function and shortened telomeres. Through directed differentiation, we generated motor neurons and astrocytes to investigate whether telomere shortening could lead to age-associated phenotypes. Our findings revealed that shortened telomeres induced age-associated characteristics in both motor neurons and astrocytes including increased cellular senescence, heightened inflammation, and elevated DNA damage. We also observed cell-type specific age-related morphology changes. Additionally, our study highlighted the fundamental role of TERT and telomere shortening in neural progenitor cell (NPC) proliferation and neuronal differentiation. This study serves as a proof of concept that telomere shortening can effectively induce aging-associated phenotypes, thereby providing a valuable tool to investigate age-related decline and neurodegenerative diseases.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"341-360"},"PeriodicalIF":4.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10998800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138175505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome ends and the theory of marginotomy: implications for reproduction. 染色体末端与边缘切除理论：对生殖的启示。

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-01 Epub Date: 2023-11-09 DOI: 10.1007/s10522-023-10071-w

Isabel Córdova-Oriz, Alba M Polonio, Isabel Cuadrado-Torroglosa, Lucía Chico-Sordo, Marta Medrano, Juan A García-Velasco, Elisa Varela

{"title":"Chromosome ends and the theory of marginotomy: implications for reproduction.","authors":"Isabel Córdova-Oriz, Alba M Polonio, Isabel Cuadrado-Torroglosa, Lucía Chico-Sordo, Marta Medrano, Juan A García-Velasco, Elisa Varela","doi":"10.1007/s10522-023-10071-w","DOIUrl":"10.1007/s10522-023-10071-w","url":null,"abstract":"Telomeres are the protective structures located at the ends of linear chromosomes. They were first described in the 1930s, but their biology remained unexplored until the early 70s, when Alexey M. Olovnikov, a theoretical biologist, suggested that telomeres cannot be fully copied during DNA replication. He proposed a theory that linked this phenomenon with the limit of cell proliferation capacity and the \"duration of life\" (theory of marginotomy), and suggested a potential of telomere lenghthening for the prevention of aging (anti-marginotomy). The impact of proliferative telomere shortening on life expectancy was later confirmed. In humans, telomere shortening is counteracted by telomerase, an enzyme that is undetectable in most adult somatic cells, but present in cancer cells and adult and embryonic stem and germ cells. Although telomere length dynamics are different in male and female gametes during gametogenesis, telomere lengths are reset at the blastocyst stage, setting the initial length of the species. The role of the telomere pathway in reproduction has been explored for years, mainly because of increased infertility resulting from delayed childbearing. Short telomere length in ovarian somatic cells is associated to decreased fertility and higher aneuploidy rates in embryos. Consequently, there is a growing interest in telomere lengthening strategies, aimed at improving fertility. It has also been observed that lifestyle factors can affect telomere length and improve fertility outcomes. In this review, we discuss the implications of telomere theory in fertility, especially in oocytes, spermatozoa, and embryos, as well as therapies to enhance reproductive success.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"227-248"},"PeriodicalIF":4.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71520370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telomeres and aging: on and off the planet! 端粒与衰老：地球上和地球外！

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-01 DOI: 10.1007/s10522-024-10098-7

{"title":"Telomeres and aging: on and off the planet!","authors":"","doi":"10.1007/s10522-024-10098-7","DOIUrl":"https://doi.org/10.1007/s10522-024-10098-7","url":null,"abstract":"<h3>Abstract</h3> Improving human healthspan in our rapidly aging population has never been more imperative. Telomeres, protective “caps” at the ends of linear chromosomes, are essential for maintaining genome stability of eukaryotic genomes. Due to their physical location and the “end-replication problem” first envisioned by Dr. Alexey Olovnikov, telomeres shorten with cell division, the implications of which are remarkably profound. Telomeres are hallmarks and molecular drivers of aging, as well as fundamental integrating components of the cumulative effects of genetic, lifestyle, and environmental factors that erode telomere length over time. Ongoing telomere attrition and the resulting limit to replicative potential imposed by cellular senescence serves a powerful tumor suppressor function, and also underlies aging and a spectrum of age-related degenerative pathologies, including reduced fertility, dementias, cardiovascular disease and cancer. However, very little data exists regarding the extraordinary stressors and exposures associated with long-duration space exploration and eventual habitation of other planets, nor how such missions will influence telomeres, reproduction, health, disease risk, and aging. Here, we briefly review our current understanding, which has advanced significantly in recent years as a result of the NASA Twins Study, the most comprehensive evaluation of human health effects associated with spaceflight ever conducted. Thus, the Twins Study is at the forefront of personalized space medicine approaches for astronauts and sets the stage for subsequent missions. We also extrapolate from current understanding to future missions, highlighting potential biological and biochemical strategies that may enable human survival, and consider the prospect of longevity in the extreme environment of space.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"62 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140590947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telomeres, cellular senescence, and aging: past and future. 端粒、细胞衰老与衰老：过去与未来。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-04-01 Epub Date: 2023-12-27 DOI: 10.1007/s10522-023-10085-4

Madeline Eppard, João F Passos, Stella Victorelli

引用次数: 0

Telomeres in health and longevity: special issue in memory of Alexey Olovnikov. 健康与长寿中的端粒：纪念阿列克谢-奥洛夫尼科夫特刊。

IF 4.5 4区医学

Biogerontology Pub Date : 2024-04-01 DOI: 10.1007/s10522-023-10090-7

Ivan A Olovnikov

引用次数: 0