Biogerontology最新文献

{"title":"Investigating cognitive impairments and hippocampal proteome alterations in aged male rats with TAA-Induced minimal hepatic encephalopathy.","authors":"Vishal Vikram Singh, Shambhu Kumar Prasad, Arup Acharjee, Sanjeeva Srivastava, Papia Acharjee","doi":"10.1007/s10522-024-10158-y","DOIUrl":"https://doi.org/10.1007/s10522-024-10158-y","url":null,"abstract":"<p><p>The aging population faces a gradual decline in physical and mental capacities, with an increased risk of liver cirrhosis and chronic liver diseases leading to hepatic encephalopathy (HE). The intertwining of physiological manifestations of aging with the pathophysiology of HE significantly impairs cognitive ability, reduces quality of life, and increases mortality. Hence, effective therapeutic intervention is imperative. The present study investigated the impact of minimal HE (MHE) on cognitive impairment in an aged rat population by analyzing hippocampal proteome dynamics. For this purpose, an old MHE rat model was induced via thioacetamide. The label-free LC‒MS/MS method was employed to explore hippocampal proteomic changes and associated dysregulated biological pathways. A total of 1533 proteins were identified, and among these, 30 proteins were significantly differentially expressed (18 upregulated, and 12 downregulated). Three upregulated proteins, namely, fetuin-A, p23, and intersectin-1 were selected and validated for their increased expression via western blotting and immunofluorescence analysis, which confirmed the mass spectrometry results. These proteins have not been reported previously in MHE cases. We also identified the possible dysregulated biological pathways associated with the differentially expressed proteins via Metascape, a network analysis tool. We found that the differentially expressed proteins may be involved in the generation of precursor metabolites and energy, the neurotransmitter release cycle, positive regulation of dendritic spine development, chaperone-mediated protein folding and protein stabilization. This study highlights the potential mechanisms underlying neurological dysfunction in the aged population with MHE and identifies novel therapeutic targets for improved disease management.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"30"},"PeriodicalIF":4.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin preserves mitochondria-endoplasmic reticulum contact sites improving mitochondrial dynamics in aged myocardial cells.","authors":"Ray Jiménez, Alejandra Zúñiga-Muñoz, Edith Álvarez-León, Wylly Ramsés García-Niño, Gabriela Navarrete-Anastasio, Elizabeth Soria-Castro, Israel Pérez-Torres, Elizabeth Lira-Silva, Natalia Pavón, Alfredo Cruz-Gregorio, Rebeca López-Marure, Cecilia Zazueta, Alejandro Silva-Palacios","doi":"10.1007/s10522-024-10174-y","DOIUrl":"https://doi.org/10.1007/s10522-024-10174-y","url":null,"abstract":"<p><p>Cardiomyocyte senescence plays a crucial role in the pathophysiology of age-related cardiovascular disease. Senescent cells with impaired contractility, mitochondrial dysfunction, and hypertrophic growth accumulate in the heart during aging, contributing to cardiac dysfunction and remodeling. Mitochondrial dynamics is altered in aging cells, leading to changes in their function and morphology. Such rearrangements can affect the spatially restricted region of the mitochondrial membrane that interacts with reticulum membrane fragments, termed mitochondria-endoplasmic reticulum (ER) contact sites (MERCs). Besides, oxidative stress associated with inefficient organelle turnover can drive cellular senescence. Therefore, in this study, we evaluated the possible association between the senolytic effect of the antioxidant quercetin (Q) and MERCs preservation in a D-galactose-induced cellular senescence model. We found that Q ameliorates the senescent phenotype of H9c2 cells in association with increased mitochondria-ER colocalization, reduced distance between both organelles, and lower ROS production. Moreover, regulation of fusion and fission processes was related with increased mitochondrial ATP production and enhanced transmembrane potential. Overall, our data provide evidence that the inhibitory effect of Q on cellular senescence is associated with preserved MERCs and improved mitochondrial function and morphology, which might contribute to the attenuation of cardiac dysfunction.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"29"},"PeriodicalIF":4.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ribose-induced advanced glycation end products reduce the lifespan in Drosophila melanogaster by changing the redox state and down-regulating the Sirtuin genes.","authors":"Lokanath Mishra, Monalisa Mishra","doi":"10.1007/s10522-024-10172-0","DOIUrl":"https://doi.org/10.1007/s10522-024-10172-0","url":null,"abstract":"<p><p>Advanced Glycation End (AGE) products are one such factor that accumulates during aging and age-related diseases. However, how exogenous AGE compounds cause aging is an area that needs to be explored. Specifically, how an organ undergoes aging and aging-related phenomena that need further investigation. The intestine is the most exposed area to food substances. How AGEs affect the intestine in terms of aging need to be explored. Drosophila melanogaster, a well-known model organism, is used to decode aging and age-associated phenomena. In this study, we fed Ribose induced Advanced Glycation End products (Rib-AGE) to D. melanogaster to study the aging mechanism. The Rib-AGE-induced aging was checked in Drosophila. We found a series of changes in Rib-AGE-fed flies. Reactive oxygen species (ROS) and nitric oxide species (NOs) were higher in the Rib-AGE-fed flies, and the antioxidant level was lower. The intestinal permeability was altered. The microorganism load was higher inside the gut. The structural arrangement of the gut's microfilament was found to be damaged, and the nuclear shape was found to be irregular. Cell death within the gut was elevated in comparison to control. The food intake was found to be reduced. The relative mRNA expression of the Sirtuin 2 and Sirtuin 6 gene of D. melanogaster was downregulated in Rib-AGE-fed flies compared to the control. All these findings strongly suggest that Rib-AGE accelerates aging and age-related disorders in D. melanogaster.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"28"},"PeriodicalIF":4.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2,4-Dinitrophenol is toxic on a low caloric diet but extends lifespan of Drosophila melanogaster on nutrient-rich diets without an impact on metabolism.","authors":"Olha M Strilbytska, Uliana Semaniuk, Ihor Yurkevych, Vladyslav Berezovskyi, Andriy Glovyak, Dmytro V Gospodaryov, Maria M Bayliak, Oleh Lushchak","doi":"10.1007/s10522-024-10169-9","DOIUrl":"https://doi.org/10.1007/s10522-024-10169-9","url":null,"abstract":"<p><p>Uncouplers of mitochondrial electron transport chain, such as 2,4-dinitrophehol (DNP), can mimic calorie restriction by decreasing efficiency of adenosine triphosphate (ATP) synthesis. However, DNP is also a toxic substance, whose overdosage can be lethal. In the fruit fly, Drosophila melanogaster model, we have found that DNP in concentrations of 0.05-0.2 g/L, led to a drastic decrease in fruit fly survival on a low caloric diet (1% sucrose and 1% yeast; 1S-1Y). On the 5S-5Y diet, DNP decreased lifespan of flies reared only in concentration 0.2 g/L, whilst on the diet 15S-15Y DNP either did not significantly shortened fruit fly lifespan or extended it. The lifespan extension on the high caloric 15S-15Y diet with DNP was accompanied by lower activity of lactate dehydrogenase and a decrease in activities of mitochondrial respiratory chain complexes I, II, and V, determined by blue native electrophoresis followed by in-gel activity assays. The exposure to DNP also did not affect key glycolytic enzymes, antioxidant and related enzymes, and markers of oxidative stress, such as aconitase activity and amount protein carbonyls. Consumption of DNP-supplemented diet did not affect flies' resistance to heat stress, though made male flies slightly more resistant to starvation compared with males reared on the control food. We also did not observe substantial changes in the contents of metabolic stores, triacylglycerols and glycogen, in the DNP-treated flies. All this suggest that a nutrient-rich diets provide effective protection against DNP, providing a mild uncoupling of the respiratory chain that allows lifespan extension without considerable changes in metabolism.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"27"},"PeriodicalIF":4.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise, hormesis and ageing: a new section in Biogerontology.","authors":"Zsolt Radak, Suresh I S Rattan","doi":"10.1007/s10522-024-10170-2","DOIUrl":"https://doi.org/10.1007/s10522-024-10170-2","url":null,"abstract":"","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"26"},"PeriodicalIF":4.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coniferaldehyde activates autophagy and enhances oxidative stress resistance and lifespan of Caenorhabditis elegans via par-4/aak-2/skn-1 pathway.","authors":"Mahesh Ramatchandirane, Ponsankaran Rajendran, M P Athira, Kitlangki Suchiang","doi":"10.1007/s10522-024-10163-1","DOIUrl":"https://doi.org/10.1007/s10522-024-10163-1","url":null,"abstract":"<p><p>Aging represents the gradual accumulation of alterations within an organism over time. The physical and chemical characteristics of our cells gradually change as we age, making it more difficult for our tissues and organs to self-regulate, regenerate, and maintain their structural and functional integrity. AMP- activated protein kinase (AMPK), a well-known sensor of cellular energy status acts as a central regulator of an integrated signalling network that control homeostasis, metabolism, stress resistance, cell survival and autophagy. Coniferaldehyde (CFA), a phenolic compound found in many edible plants, has multiple biological and pharmacological functions. Our findings demonstrated that 50 µM CFA could significantly activate autophagy and reduce oxidative stress, which enhanced the activity of antioxidant enzymes and increased resistance under oxidative stress. CFA treatment could efficiently decrease reactive oxygen species (ROS) levels and positively enhance the expression of antioxidant genes in Caenorhabditis elegans (C. elegans). On the other hand, CFA did not have any role in the lifespan extension of the several mutants linked to the AAK-2/AMPK pathway and it promotes SKN-1 (Skinhead-1) localization into the nucleus, which modulates downstream gene gst-4 (Glutathione S-transferase). In depth investigations revealed that CFA could lower oxidative stress and enhance the lifespan of C. elegans by activating the PAR-4/LKB-1-AAK-2/AMPK-SKN-1/NRF-2 pathway, with crucial involvement of bec-1 and lgg-1 genes for autophagy mediated lifespan extension. This study might contribute to understanding the interactions and mechanisms that allow natural compounds like CFA to treat age-related disorders among several species.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"25"},"PeriodicalIF":4.4,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between dietary carotenoid and biological age acceleration: insights from NHANES 2009-2018.","authors":"Xinyun Chen, Chunying He, Wenhui Yu, Liang Ma, Shenju Gou, Ping Fu","doi":"10.1007/s10522-024-10160-4","DOIUrl":"https://doi.org/10.1007/s10522-024-10160-4","url":null,"abstract":"<p><p>Carotenoids are naturally occurring pigments found in plants and certain microorganisms. Some carotenoids act as precursors to vitamin A, which is essential for various health aspects, including vision, immune function, and skin health. Carotenoids, including α-carotene, β-carotene, β-cryptoxanthin, lycopene, lutein and zeaxanthin, are known to reduce the risk of age-related diseases and promote healthy aging. This study examines the relationship between dietary carotenoid levels and biological age. This study utilized data from the National Health and Nutrition Examination Survey (NHANES) from 2009 to 2018, and 19,280 participants were included. The Phenotypic Age (PhenoAge) was used to measure biological age, and the Klemera-Doubal Method (KDM) was employed in sensitivity analyses. Biological age acceleration was determined by calculating the residuals of PhenoAge or KDM after regressing them against chronological age. Weighted multivariate linear and logistic regressions were conducted to examine the relationship between carotenoids and biological age acceleration. Additionally, restricted cubic spline regression, subgroup analysis, interaction analysis, and sensitivity analyses were employed for further examination. Both linear regression and logistic regression analyses indicated that participants with higher carotenoid intake exhibited lower rates of phenotypic age acceleration, with α-carotene, β-carotene, β-cryptoxanthin, lutein and zeaxanthin, and lycopene all demonstrating protective effects. Restricted cubic spline regression indicates non-linear associations between carotenoid levels and phenotypic age acceleration. Subgroup analyses revealed that younger participants, females, and individuals with hypertension or diabetes benefited more from higher carotenoid intake. Sensitivity analyses further confirmed the robustness of inverse relationship. The WQS analysis identifies β-carotene and β-cryptoxanthin as the most influential compounds. Higher dietary intake of carotenoids is associated with reduced biological age acceleration, underscoring their protective role against aging. Further longitudinal studies are needed to establish causal relationships and explore the underlying mechanisms of carotenoid benefits on aging.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"24"},"PeriodicalIF":4.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of differentially expressed miRNAs involved in vascular aging reveals pathways associated with the endocrine hormone regulation.","authors":"Jeongwon Jeon, Subin Jang, Ki-Soo Park, Han-Gyul Kim, Jongan Lee, Tae-Sung Hwang, Jin-Sin Koh, Jaemin Kim","doi":"10.1007/s10522-024-10167-x","DOIUrl":"10.1007/s10522-024-10167-x","url":null,"abstract":"<p><p>Vascular aging refers to a series of processes where the elasticity of blood vessels diminishes, leading to stiffening, and deposition of fat components on the vessel walls, causing inflammation. Cardiovascular diseases, such as stroke and hypertension, play significant roles in morbidity and mortality rates among the elderly population. In this study, the Reactive Hyperemia Index (RHI) was measured to assess vascular endothelial function and aging-induced pathogenesis of vascular diseases in Korean subjects. We aimed to identify extracellular vesicle microRNAs (EV-miRNAs) with differential abundance between groups of individuals at the ends of a continuum in vascular aging acceleration, revealing miRNAs regulating genes in endocrine hormone regulation and tumor-related pathways. We also discovered that the principal component characterizing the global miRNA expression profile is significantly associated with clinical traits including cholesterol levels. Together, these data provide a foundation for understanding the role of miRNAs as modulators of longevity and for developing age-specific epigenetic biomarkers.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"23"},"PeriodicalIF":4.4,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDF-15 as a proxy for epigenetic aging: associations with biological age markers, and physical function.","authors":"Margalida Torrens-Mas, Cayetano Navas-Enamorado, Aina Galmes-Panades, Luis Masmiquel, Andrés Sanchez-Polo, Xavier Capo, Marta Gonzalez-Freire","doi":"10.1007/s10522-024-10165-z","DOIUrl":"10.1007/s10522-024-10165-z","url":null,"abstract":"<p><p>Growth differentiation factor 15 (GDF-15) has emerged as a significant biomarker of aging, linked to various physiological and pathological processes. This study investigates circulating GDF-15 levels in a cohort of healthy individuals from the Balearic Islands, exploring its associations with biological age markers, including multiple DNA methylation (DNAm) clocks, physical performance, and other age-related biomarkers. Seventy-two participants were assessed for general health, body composition, and physical function, with GDF-15 levels quantified using ELISA. Our results indicate that GDF-15 levels significantly increase with age, particularly in individuals over 60. Strong positive correlations were observed between GDF-15 levels and DNAm GrimAge, DNAm PhenoAge, Hannum, and Zhang clocks, suggesting that GDF-15 could serve as a proxy for epigenetic aging. Additionally, GDF-15 levels were linked to markers of impaired glycemic control, systemic inflammation, and physical decline, including decreased lung function and grip strength, especially in men. These findings highlight the use of GDF-15 as a biomarker for aging and age-related functional decline. Given that GDF-15 is easier to measure than DNA methylation, it has the potential to be more readily implemented in clinical settings for broader health assessment and management.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"22"},"PeriodicalIF":4.4,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of serum metabolites with frailty phenotype and its components: a cross-sectional case-control study.","authors":"Takashi Shida, Sho Hatanaka, Narumi Kojima, Takahisa Ohta, Yosuke Osuka, Kazushi Maruo, Hiroyuki Sasai","doi":"10.1007/s10522-024-10166-y","DOIUrl":"https://doi.org/10.1007/s10522-024-10166-y","url":null,"abstract":"<p><p>New insights into the metabolic mechanisms of frailty are needed. This study aimed to identify serum metabolites linked to frailty phenotype and its component through gas chromatography-mass spectrometry metabolomic analysis among community-dwelling older individuals. An exploratory, cross-sectional case-control study. Setting and participants: The participants were recruited from the ''Otassha Study,'' a cohort study conducted in Itabashi Ward, Tokyo, targeting women aged 65 years and older. The study population included 39 frail and 76 robust individuals. Metabolomic analysis was performed using the GCMS-TQ^TM8040 NX system and the Smart Metabolites Database Ver.2 to explore the primary metabolite characteristic of a frailty state. Conditional logistic regression analysis was conducted with frailty as the outcome and with metabolites as exposures. Concentrations of seven metabolites, including caffeine, catechol, paraxanthine, niacinamide, 5-hydroxymethyl-2-furoic acid, daidzein, and cytosine were lower in the frail than in the robust individuals. Odds ratios [95% confidence intervals] for frailty by halving the value were significant for catechol (1.26 [1.00, 1.59]), 5-hydroxymethyl-2-furoic acid (1.28 [1.04, 1.58]), caffeine (1.37 [1.07, 1.75]), paraxanthine (1.18 [1.00, 1.39]), and daidzein (1.29 [1.02, 1.62]). Furthermore, distinct patterns of metabolites associated with specific frailty symptoms, such as muscle weakness, fatigue, and reduced physical activity, were identified, especially with 5-hydroxymethyl-2-furoic acid and caffeine commonly associated with these components. Metabolomic analysis identified metabolites associated with frailty. In particular, low levels of caffeine, catechol, paraxanthine, niacinamide, 5-hydroxymethyl-2-furoate, daidzein, and cytosine contributed to frailty. These results provide new insights into the pathophysiology of frailty through metabolomic analysis.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 1","pages":"21"},"PeriodicalIF":4.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}