Biological Chemistry最新文献_第4页

Platelet-derived growth factor-stimulated pulmonary artery smooth muscle cells regulate pulmonary artery endothelial cell dysfunction through extracellular vesicle miR-409-5p. 血小板衍生生长因子刺激的肺动脉平滑肌细胞通过细胞外小泡miR-409-5p调节肺动脉内皮细胞功能障碍。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-10-31 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0222

Jeongyeon Heo, Hara Kang

{"title":"Platelet-derived growth factor-stimulated pulmonary artery smooth muscle cells regulate pulmonary artery endothelial cell dysfunction through extracellular vesicle miR-409-5p.","authors":"Jeongyeon Heo, Hara Kang","doi":"10.1515/hsz-2023-0222","DOIUrl":"10.1515/hsz-2023-0222","url":null,"abstract":"Platelet-derived growth factor (PDGF)-induced changes in vascular smooth muscle cells (VSMCs) stimulate vascular remodeling, resulting in vascular diseases such as pulmonary arterial hypertension. VSMCs communicate with endothelial cells through extracellular vesicles (EVs) carrying cargos, including microRNAs. To understand the molecular mechanisms through which PDGF-stimulated pulmonary artery smooth muscle cells (PASMCs) interact with pulmonary artery endothelial cells (PAECs) under pathological conditions, we investigated the crosstalk between PASMCs and PAECs via extracellular vesicle miR-409-5p under PDGF stimulation. miR-409-5p expression was upregulated in PASMCs upon PDGF signaling, and it was released into EVs. The elevated expression of miR-409-5p was transported to PAECs and led to their impaired function, including reduced NO release, which consequentially resulted in enhanced PASMC proliferation. We propose that the positive regulatory loop of PASMC-extracellular vesicle miR-409-5p-PAEC is a potential mechanism underlying the proliferation of PASMCs under PDGF stimulation. Therefore, miR-409-5p may be a novel therapeutic target for the treatment of vascular diseases, including pulmonary arterial hypertension.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"203-215"},"PeriodicalIF":2.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer. 组织蛋白酶L介导的EGFR切割影响癌症细胞内信号通路。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-10-30 Print Date: 2024-04-25 DOI: 10.1515/hsz-2023-0213

Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović

{"title":"Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer.","authors":"Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović","doi":"10.1515/hsz-2023-0213","DOIUrl":"10.1515/hsz-2023-0213","url":null,"abstract":"Proteolytic activity in the tumour microenvironment is an important factor in cancer development since it can also affect intracellular signalling pathways via positive feedback loops that result in either increased tumour growth or resistance to anticancer mechanisms. In this study, we demonstrated extracellular cathepsin L-mediated cleavage of epidermal growth factor receptor (EGFR) and identified the cleavage site in the extracellular domain after R224. To further evaluate the relevance of this cleavage, we cloned and expressed a truncated version of EGFR, starting at G225, in HeLa cells. We confirmed the constitutive activation of the truncated protein in the absence of ligand binding and determined possible changes in intracellular signalling. Furthermore, we determined the effect of truncated EGFR protein expression on HeLa cell viability and response to the EGFR inhibitors, tyrosine kinase inhibitor (TKI) erlotinib and monoclonal antibody (mAb) cetuximab. Our data reveal the nuclear localization and phosphorylation of EGFR and signal trancducer and activator of transcription 3 (STAT3) in cells that express the truncated EGFR protein and suggest that these phenomena cause resistance to EGFR inhibitors.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"283-296"},"PeriodicalIF":2.9,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61560302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of yeast RNA polymerase I with special consideration of the lobe binding subunits. 酵母核糖核酸聚合酶I的特征，特别考虑了叶结合亚基。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-13 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0184

Katrin Schwank, Catharina Schmid, Tobias Fremter, Christoph Engel, Philipp Milkereit, Joachim Griesenbeck, Herbert Tschochner

引用次数: 0

Towards correlative archaeology of the human mind. 走向人类心灵的相关考古学。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-12 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0199

Lukasz Piszczek, Joanna Kaczanowska, Wulf Haubensak

引用次数: 0

The good or the bad: an overview of autoantibodies in traumatic spinal cord injury. 好的或坏的：创伤脊髓损伤中自身抗体的概述。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-03 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0252

Annika Guntermann, Katrin Marcus, Caroline May

引用次数: 0

Frontmatter 头版头条

4区生物学

Biological Chemistry Pub Date : 2023-10-01 DOI: 10.1515/hsz-2023-frontmatter11-12

引用次数: 0

RBPome identification in egg-cell like callus of Arabidopsis. 拟南芥卵细胞样愈伤组织中RBPome的鉴定。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-29 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0195

Liping Liu, Jakob Trendel, Guojing Jiang, Yanhui Liu, Astrid Bruckmann, Bernhard Küster, Stefanie Sprunck, Thomas Dresselhaus, Andrea Bleckmann

{"title":"RBPome identification in egg-cell like callus of Arabidopsis.","authors":"Liping Liu, Jakob Trendel, Guojing Jiang, Yanhui Liu, Astrid Bruckmann, Bernhard Küster, Stefanie Sprunck, Thomas Dresselhaus, Andrea Bleckmann","doi":"10.1515/hsz-2023-0195","DOIUrl":"10.1515/hsz-2023-0195","url":null,"abstract":"RNA binding proteins (RBPs) have multiple and essential roles in transcriptional and posttranscriptional regulation of gene expression in all living organisms. Their biochemical identification in the proteome of a given cell or tissue requires significant protein amounts, which limits studies in rare and highly specialized cells. As a consequence, we know almost nothing about the role(s) of RBPs in reproductive processes such as egg cell development, fertilization and early embryogenesis in flowering plants. To systematically identify the RBPome of egg cells in the model plant Arabidopsis, we performed RNA interactome capture (RIC) experiments using the egg cell-like RKD2-callus and were able to identify 728 proteins associated with poly(A+)-RNA. Transcripts for 97 % of identified proteins could be verified in the egg cell transcriptome. 46 % of identified proteins can be associated with the RNA life cycle. Proteins involved in mRNA binding, RNA processing and metabolism are highly enriched. Compared with the few available RBPome datasets of vegetative plant tissues, we identified 475 egg cell-enriched RBPs, which will now serve as a resource to study RBP function(s) during egg cell development, fertilization and early embryogenesis. First candidates were already identified showing an egg cell-specific expression pattern in ovules.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"1137-1149"},"PeriodicalIF":3.7,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1-RAGE axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte autophagy and apoptosis in diabetic mice. HMGB1-RAGE轴通过调节糖尿病小鼠的心肌细胞自噬和凋亡，促进心肌缺血/再灌注损伤。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-28 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0134

De-Wei He, De-Zhao Liu, Xiao-Zhi Luo, Chuan-Bin Chen, Chuang-Hong Lu, Na Na, Feng Huang

{"title":"HMGB1-RAGE axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte autophagy and apoptosis in diabetic mice.","authors":"De-Wei He, De-Zhao Liu, Xiao-Zhi Luo, Chuan-Bin Chen, Chuang-Hong Lu, Na Na, Feng Huang","doi":"10.1515/hsz-2023-0134","DOIUrl":"10.1515/hsz-2023-0134","url":null,"abstract":"Patients with acute myocardial infarction complicated with diabetes are more likely to develop myocardial ischemia/reperfusion (I/R) injury (MI/RI) during reperfusion therapy. Both HMGB1 and RAGE play important roles in MI/RI. However, the specific mechanisms of HMGB1 associated with RAGE are not fully clarified in diabetic MI/RI. This study aimed to investigate whether the HMGB1-RAGE axis induces diabetic MI/RI via regulating autophagy and apoptosis. A db/db mouse model of MI/RI was established, where anti-HMGB1 antibody and RAGE inhibitor (FPS-ZM1) were respectively injected after 10 min of reperfusion. The results showed that treatment with anti-HMGB1 significantly reduced the infarct size, serum LDH, and CK-MB level. Similar situations also occurred in mice administrated with FPS-ZM1, though the HMGB1 level was unchanged. Then, we found that treatment with anti-HMGB1 or FPS-ZM1 performed the same effects in suppressing the autophagy and apoptosis, as reflected by the results of lower LAMP2 and LC3B levels, increased Bcl-2 level, reduced BAX and caspase-3 levels. Moreover, the Pink1/Parkin levels were also inhibited at the same time. Collectively, this study indicates that the HMGB1-RAGE axis aggravated diabetic MI/RI via apoptosis and Pink1/Parkin mediated autophagy pathways, and inhibition of HMGB1 or RAGE contributes to alleviating those adverse situations.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"167-176"},"PeriodicalIF":2.9,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41109563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highlight: how to synthesize, assemble and regulate ribonucleoprotein-complexes. 推荐理由：如何合成、组装和调节核糖核蛋白复合物。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-27 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0301

Herbert Tschochner

引用次数: 0

Post-transcriptional gene silencing in a dynamic RNP world. 动态RNP世界中的转录后基因沉默。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-25 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0203

Simone Larivera, Julia Neumeier, Gunter Meister

引用次数: 0