Biological Chemistry最新文献_第5页

Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation. 硝基油酸及其与伽马射线照射结合的细胞特异性抗癌作用。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-15 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0150

Tomas Perecko, Jana Pereckova, Zuzana Hoferova, Martin Falk

{"title":"Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation.","authors":"Tomas Perecko, Jana Pereckova, Zuzana Hoferova, Martin Falk","doi":"10.1515/hsz-2023-0150","DOIUrl":"10.1515/hsz-2023-0150","url":null,"abstract":"Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO2OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO2OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50 % survival fraction (DERIC50) was calculated for samples pre-treated with NO2OA followed by gamma irradiation. NO2OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO2OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO2OA prior irradiation showed a significantly increased DERIC50, demonstrating radiosensitizing effects. In conclusion, NO2OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"177-187"},"PeriodicalIF":2.9,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A structural biology view on the enzymes involved in eukaryotic mRNA turnover. 真核生物mRNA转换酶的结构生物学观点。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-15 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0182

Christina Krempl, Daniela Lazzaretti, Remco Sprangers

{"title":"A structural biology view on the enzymes involved in eukaryotic mRNA turnover.","authors":"Christina Krempl, Daniela Lazzaretti, Remco Sprangers","doi":"10.1515/hsz-2023-0182","DOIUrl":"10.1515/hsz-2023-0182","url":null,"abstract":"The cellular environment contains numerous ribonucleases that are dedicated to process mRNA transcripts that have been targeted for degradation. Here, we review the three dimensional structures of the ribonuclease complexes (Pan2-Pan3, Ccr4-Not, Xrn1, exosome) and the mRNA decapping enzymes (Dcp2, DcpS) that are involved in mRNA turnover. Structures of major parts of these proteins have been experimentally determined. These enzymes and factors do not act in isolation, but are embedded in interaction networks which regulate enzyme activity and ensure that the appropriate substrates are recruited. The structural details of the higher order complexes that form can, in part, be accurately deduced from known structural data of sub-complexes. Interestingly, many of the ribonuclease and decapping enzymes have been observed in structurally different conformations. Together with experimental data, this highlights that structural changes are often important for enzyme function. We conclude that the known structural data of mRNA decay factors provide important functional insights, but that static structural data needs to be complemented with information regarding protein motions to complete the picture of how transcripts are turned over. In addition, we highlight multiple aspects that influence mRNA turnover rates, but that have not been structurally characterized so far.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"1101-1121"},"PeriodicalIF":3.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10608946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The archaeal Lsm protein from Pyrococcus furiosus binds co-transcriptionally to poly(U)-rich target RNAs. 来自发热热球菌的古生菌Lsm蛋白通过共转录与多U富靶rna结合。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-15 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0215

Robert Reichelt, Tamara Rothmeier, Felix Grünberger, Sarah Willkomm, Astrid Bruckmann, Winfried Hausner, Dina Grohmann

{"title":"The archaeal Lsm protein from Pyrococcus furiosus binds co-transcriptionally to poly(U)-rich target RNAs.","authors":"Robert Reichelt, Tamara Rothmeier, Felix Grünberger, Sarah Willkomm, Astrid Bruckmann, Winfried Hausner, Dina Grohmann","doi":"10.1515/hsz-2023-0215","DOIUrl":"10.1515/hsz-2023-0215","url":null,"abstract":"Posttranscriptional processes in Bacteria include the association of small regulatory RNAs (sRNA) with a target mRNA. The sRNA/mRNA annealing process is often mediated by an RNA chaperone called Hfq. The functional role of bacterial and eukaryotic Lsm proteins is partially understood, whereas knowledge about archaeal Lsm proteins is scarce. Here, we used the genetically tractable archaeal hyperthermophile Pyrococcus furiosus to identify the protein interaction partners of the archaeal Sm-like proteins (PfuSmAP1) using mass spectrometry and performed a transcriptome-wide binding site analysis of PfuSmAP1. Most of the protein interaction partners we found are part of the RNA homoeostasis network in Archaea including ribosomal proteins, the exosome, RNA-modifying enzymes, but also RNA polymerase subunits, and transcription factors. We show that PfuSmAP1 preferentially binds messenger RNAs and antisense RNAs recognizing a gapped poly(U) sequence with high affinity. Furthermore, we found that SmAP1 co-transcriptionally associates with target RNAs. Our study reveals that in contrast to bacterial Hfq, PfuSmAP1 does not affect the transcriptional activity or the pausing behaviour of archaeal RNA polymerases. We propose that PfuSmAP1 recruits antisense RNAs to target mRNAs and thereby executes its putative regulatory function on the posttranscriptional level.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"1085-1100"},"PeriodicalIF":3.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10242690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The promise of genetic screens in human in vitro brain models. 体外人脑模型基因筛选的前景。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-12 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0174

Julianne Beirute-Herrera, Beatriz López-Amo Calvo, Frank Edenhofer, Christopher Esk

引用次数: 0

N6-methyladenosine-induced METTL1 promotes tumor proliferation via CDK4. N6-甲基腺苷诱导的 METTL1 通过 CDK4 促进肿瘤增殖。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-12 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0260

Chunyan Zhang, Yuanbo Cui

{"title":"N6-methyladenosine-induced METTL1 promotes tumor proliferation via CDK4.","authors":"Chunyan Zhang, Yuanbo Cui","doi":"10.1515/hsz-2023-0260","DOIUrl":"10.1515/hsz-2023-0260","url":null,"abstract":"N6-methyladenosine (m6A) and N7-methylguanosine (m7G) modification of RNA represent two major intracellular post-transcriptional regulation modes of gene expression. However, the crosstalk of these two epigenetic modifications in tumorigenesis remain poorly understood. Here, we show that m6A methyltransferase METTL3-mediated METTL1 promotes cell proliferation of head and neck squamous cell carcinoma (HNSC) through m7G modification of the cell-cycle regulator CDK4. By mining the database GEPIA, METTL1 was shown to be up-regulated in a broad spectrum of human cancers and correlated with patient clinical outcomes, particularly in HNSC. Mechanistically, METTL3 methylates METTL1 mRNA and mediates its elevation in HNSC via m6A. Functionally, over-expression of METTL1 enhances HNSC cell growth and facilitates cell-cycle progress, while METTL1 knockdown represses these biological behaviors. Moreover, METTL1 physically binds to CDK4 transcript and regulates its m7G modification level to stabilize CDK4. Importantly, the inhibitory effects of METTL1 knockdown on the proliferation of HNSC, esophageal cancer (ESCA), stomach adenocarcinoma (STAD), and colon adenocarcinoma (COAD) were significantly mitigated by over-expression of CDK4. Taken together, this study expands the understanding of epigenetic mechanisms involved in tumorigenesis and identifies the METTL1/CDK4 axis as a potential therapeutic target for digestive system tumors.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"217-228"},"PeriodicalIF":2.9,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10202840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubules as a signal hub for axon growth in response to mechanical force. 微管作为轴突生长响应机械力的信号中枢。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-08 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0173

Alessandro Falconieri, Allegra Coppini, Vittoria Raffa

引用次数: 0

The Zika virus infection remodels the expression of the synaptotagmin-9 secretory protein. 寨卡病毒感染重塑了突触标记蛋白-9分泌蛋白的表达。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-08 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0165

Santiago Leiva, Alejo Cantoia, Cintia Fabbri, Marina Bugnon Valdano, Victoria Luppo, María Alejandra Morales, Germán Rosano, Daniela Gardiol

{"title":"The Zika virus infection remodels the expression of the synaptotagmin-9 secretory protein.","authors":"Santiago Leiva, Alejo Cantoia, Cintia Fabbri, Marina Bugnon Valdano, Victoria Luppo, María Alejandra Morales, Germán Rosano, Daniela Gardiol","doi":"10.1515/hsz-2023-0165","DOIUrl":"10.1515/hsz-2023-0165","url":null,"abstract":"The exact mechanisms involved in flaviviruses virions' release and the specific secretion of viral proteins, such as the Non Structural protein-1 (NS1), are still unclear. While these processes might involve vesicular transport to the cell membrane, NS1 from some flaviviruses was shown to participate in viral assembly and release. Here, we assessed the effect of the Zika virus (ZIKV) NS1 expression on the cellular proteome to identify trafficking-related targets that may be altered in the presence of the viral protein. We detected an increase in the synaptotagmin-9 (SYT9) secretory protein, which participates in the intracellular transport of protein-laden vesicles. We confirmed the effect of NS1 on SYT9 levels by transfection models while also detecting a significant subcellular redistribution of SYT9. We found that ZIKV prM-Env proteins, required for the viral particle release, also increased SYT9 levels and changed its localization. Finally, we demonstrated that ZIKV cellular infection raises SYT9 levels and promotes changes in its subcellular localization, together with a co-distribution with both Env and NS1. Altogether, the data suggest SYT9's implication in the vesicular transport of viral proteins or virions during ZIKV infection, showing for the first time the association of synaptotagmins with the flavivirus' life cycle.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"189-201"},"PeriodicalIF":2.9,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10180542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in Arabidopsis. 拟南芥细胞质RGG rna结合蛋白是温度敏感的开花时间调节因子。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-08 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0171

Andrea Bleckmann, Nicole Spitzlberger, Philipp Denninger, Hans F Ehrnsberger, Lele Wang, Astrid Bruckmann, Stefan Reich, Philipp Holzinger, Jan Medenbach, Klaus D Grasser, Thomas Dresselhaus

{"title":"Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in Arabidopsis.","authors":"Andrea Bleckmann, Nicole Spitzlberger, Philipp Denninger, Hans F Ehrnsberger, Lele Wang, Astrid Bruckmann, Stefan Reich, Philipp Holzinger, Jan Medenbach, Klaus D Grasser, Thomas Dresselhaus","doi":"10.1515/hsz-2023-0171","DOIUrl":"10.1515/hsz-2023-0171","url":null,"abstract":"mRNA translation is tightly regulated by various classes of RNA-binding proteins (RBPs) during development and in response to changing environmental conditions. In this study, we characterize the arginine-glycine-glycine (RGG) motif containing RBP family of Arabidopsis thaliana representing homologues of the multifunctional translation regulators and ribosomal preservation factors Stm1 from yeast (ScStm1) and human SERBP1 (HsSERBP1). The Arabidopsis genome encodes three RGG proteins named AtRGGA, AtRGGB and AtRGGC. While AtRGGA is ubiquitously expressed, AtRGGB and AtRGGC are enriched in dividing cells. All AtRGGs localize almost exclusively to the cytoplasm and bind with high affinity to ssRNA, while being capable to interact with most nucleic acids, except dsRNA. A protein-interactome study shows that AtRGGs interact with ribosomal proteins and proteins involved in RNA processing and transport. In contrast to ScStm1, AtRGGs are enriched in ribosome-free fractions in polysome profiles, suggesting additional plant-specific functions. Mutant studies show that AtRGG proteins differentially regulate flowering time, with a distinct and complex temperature dependency for each AtRGG protein. In conclusion, we suggest that AtRGGs function in fine-tuning translation efficiency to control flowering time and potentially other developmental processes in response to environmental changes.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"1069-1084"},"PeriodicalIF":3.7,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10542281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoscale organization of Ca_V2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation. 突触前末端CaV2.1剪接异构体的纳米级组织：对突触小泡释放和突触促进的意义。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-04 Print Date: 2023-09-26 DOI: 10.1515/hsz-2023-0235

Lorenzo A Cingolani, Agnes Thalhammer, Fanny Jaudon, Jessica Muià, Gabriele Baj

引用次数: 0

The emerging role of ATP as a cosolute for biomolecular processes. ATP作为生物分子过程的共溶物的新兴作用。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-04 Print Date: 2023-09-26 DOI: 10.1515/hsz-2023-0202

Alexander Hautke, Simon Ebbinghaus

引用次数: 0