Biological Chemistry最新文献_第5页

Apolipoprotein E-containing lipoproteins and their extracellular interactions with LRP1 affect LPS-induced inflammation. 含载脂蛋白 E 的脂蛋白及其与 LRP1 的细胞外相互作用会影响 LPS 诱导的炎症。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-03-18 Print Date: 2024-06-25 DOI: 10.1515/hsz-2024-0018

Shogo Akahane, Hiroto Matsuura, Takahiro Kaido, Yoko Usami, Nau Ishimine, Takeshi Uehara, Kazuyoshi Yamauchi

{"title":"Apolipoprotein E-containing lipoproteins and their extracellular interactions with LRP1 affect LPS-induced inflammation.","authors":"Shogo Akahane, Hiroto Matsuura, Takahiro Kaido, Yoko Usami, Nau Ishimine, Takeshi Uehara, Kazuyoshi Yamauchi","doi":"10.1515/hsz-2024-0018","DOIUrl":"10.1515/hsz-2024-0018","url":null,"abstract":"The linkage between low-density lipoprotein receptor-related protein (LRP)1-mediated metabolism of apolipoprotein (apo) E-containing lipoproteins (apoE-LP) and the lipopolysaccharide (LPS)-induced inflammatory response contributes to the pathogenesis of sepsis; however, the underlying mechanisms are unclear. Therefore, in this study, the effects of apoE-LP and their constituents on the mRNA expression of interleukin (IL)-6 and LRP1 were evaluated using a culture system of human fibroblasts supplemented with LPS and apoE-containing emulsion particles (apoE-EP). The affinity of apoE-LP for LPS was examined using the interaction between fluorescence-labeled LPS and serum lipoprotein fractions. LPS-induced inflammation significantly upregulated the mRNA expression of IL-6 and LRP1. This upregulation was markedly suppressed by pre-incubation of LPS with apoE-EP or its constituents (apoE or EP). The suppressive effect of apoE-EP on IL-6 upregulation was attenuated in the presence of lactoferrin, an inhibitor of LRP1. The prepared apoE-EP and serum triglyceride-rich lipoproteins showed significant affinity for LPS. However, these affinities appeared to be lower than expected based on the extent to which IL-6 upregulation was suppressed by pre-incubation of LPS with apoE-EP. Overall, these results indicate that LPS-induced inflammation may be regulated by 1) the LPS-neutralizing effect of apoE-LP, 2) anti-inflammatory effect of apoE, and 3) LRP1-mediated metabolic pathways.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"383-393"},"PeriodicalIF":2.4,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of two glycosyltransferases related to polysaccharide biosynthesis in Rhodococcus jostii RHA1. 研究 Rhodococcus jostii RHA1 中与多糖生物合成有关的两种糖基转移酶。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-03-15 Print Date: 2024-05-27 DOI: 10.1515/hsz-2023-0339

Antonela Estefania Cereijo, María Victoria Ferretti, Alberto Alvaro Iglesias, Héctor Manuel Álvarez, Matías Damian Asencion Diez

{"title":"Study of two glycosyltransferases related to polysaccharide biosynthesis in Rhodococcus jostii RHA1.","authors":"Antonela Estefania Cereijo, María Victoria Ferretti, Alberto Alvaro Iglesias, Héctor Manuel Álvarez, Matías Damian Asencion Diez","doi":"10.1515/hsz-2023-0339","DOIUrl":"10.1515/hsz-2023-0339","url":null,"abstract":"The bacterial genus Rhodococcus comprises organisms performing oleaginous behaviors under certain growth conditions and ratios of carbon and nitrogen availability. Rhodococci are outstanding producers of biofuel precursors, where lipid and glycogen metabolisms are closely related. Thus, a better understanding of rhodococcal carbon partitioning requires identifying catalytic steps redirecting sugar moieties to storage molecules. Here, we analyzed two GT4 glycosyl-transferases from Rhodococcus jostii (RjoGlgAb and RjoGlgAc) annotated as α-glucan-α-1,4-glucosyl transferases, putatively involved in glycogen synthesis. Both enzymes were produced in Escherichia coli cells, purified to homogeneity, and kinetically characterized. RjoGlgAb and RjoGlgAc presented the \"canonical\" glycogen synthase activity and were actives as maltose-1P synthases, although to a different extent. Then, RjoGlgAc is a homologous enzyme to the mycobacterial GlgM, with similar kinetic behavior and glucosyl-donor preference. RjoGlgAc was two orders of magnitude more efficient to glucosylate glucose-1P than glycogen, also using glucosamine-1P as a catalytically efficient aglycon. Instead, RjoGlgAb exhibited both activities with similar kinetic efficiency and preference for short-branched α-1,4-glucans. Curiously, RjoGlgAb presented a super-oligomeric conformation (higher than 15 subunits), representing a novel enzyme with a unique structure-to-function relationship. Kinetic results presented herein constitute a hint to infer on polysaccharides biosynthesis in rhodococci from an enzymological point of view.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"325-340"},"PeriodicalIF":2.4,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yeast Bxi1/Ybh3 mediates conserved mitophagy and apoptosis in yeast and mammalian cells: convergence in Bcl-2 family. 酵母 Bxi1/Ybh3 在酵母和哺乳动物细胞中介导保守的有丝分裂和细胞凋亡：Bcl-2 家族的趋同。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-03-12 Print Date: 2024-06-25 DOI: 10.1515/hsz-2023-0359

Yuying Wang, Zhiyuan Hu, Maojun Jiang, Yanxin Zhang, Linjie Yuan, Ziqian Wang, Ting Song, Zhichao Zhang

{"title":"Yeast Bxi1/Ybh3 mediates conserved mitophagy and apoptosis in yeast and mammalian cells: convergence in Bcl-2 family.","authors":"Yuying Wang, Zhiyuan Hu, Maojun Jiang, Yanxin Zhang, Linjie Yuan, Ziqian Wang, Ting Song, Zhichao Zhang","doi":"10.1515/hsz-2023-0359","DOIUrl":"10.1515/hsz-2023-0359","url":null,"abstract":"The process of degrading unwanted or damaged mitochondria by autophagy, called mitophagy, is essential for mitochondrial quality control together with mitochondrial apoptosis. In mammalian cells, pan-Bcl-2 family members including conical Bcl-2 members and non-conical ones are involved in and govern the two processes. We have illustrated recently the BH3 receptor Hsp70 interacts with Bim to mediate both apoptosis and mitophagy. However, whether similar pathways exist in lower eukaryotes where conical Bcl-2 members are absent remained unclear. Here, a specific inhibitor of the Hsp70-Bim PPI, S1g-10 and its analogs were used as chemical tools to explore the role of yeast Bxi1/Ybh3 in regulating mitophagy and apoptosis. Using Om45-GFP processing assay, we illustrated that yeast Ybh3 mediates a ubiquitin-related mitophagy pathway in both yeast and mammalian cells through association with Hsp70, which is in the same manner with Bim. Moreover, by using Bax/Bak double knockout MEF cells, Ybh3 was identified to induce apoptosis through forming oligomerization to trigger mitochondrial outer membrane permeabilization (MOMP) like Bax. We not only illustrated a conserved ubiquitin-related mitophagy pathway in yeast but also revealed the multi-function of Ybh3 which combines the function of BH3-only protein and multi-domain Bax protein as one.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"417-426"},"PeriodicalIF":2.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Chk1 stimulates cytotoxic action of platinum-based drugs and TRAIL combination in human prostate cancer cells. 抑制 Chk1 可刺激铂类药物和 TRAIL 联合疗法对人类前列腺癌细胞的细胞毒性作用。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-03-08 Print Date: 2024-06-25 DOI: 10.1515/hsz-2023-0111

Martin Krkoška, Kamil Paruch, Tereza Šošolíková, Gerardo Vázquez-Gómez, Jarmila Herůdková, Jan Novotný, Petra Ovesná, Petr Sova, Alena Hyršlová Vaculová

{"title":"Inhibition of Chk1 stimulates cytotoxic action of platinum-based drugs and TRAIL combination in human prostate cancer cells.","authors":"Martin Krkoška, Kamil Paruch, Tereza Šošolíková, Gerardo Vázquez-Gómez, Jarmila Herůdková, Jan Novotný, Petra Ovesná, Petr Sova, Alena Hyršlová Vaculová","doi":"10.1515/hsz-2023-0111","DOIUrl":"10.1515/hsz-2023-0111","url":null,"abstract":"Checkpoint kinase 1 (Chk1) plays an important role in regulation of the cell cycle, DNA damage response and cell death, and represents an attractive target in anticancer therapy. Small-molecule inhibitors of Chk1 have been intensively investigated either as single agents or in combination with various chemotherapeutic drugs and they can enhance the chemosensitivity of numerous tumor types. Here we newly demonstrate that pharmacological inhibition of Chk1 using potent and selective inhibitor SCH900776, currently profiled in phase II clinical trials, significantly enhances cytotoxic effects of the combination of platinum-based drugs (cisplatin or LA-12) and TRAIL (tumor necrosis factor-related apoptosis inducing ligand) in human prostate cancer cells. The specific role of Chk1 in the drug combination-induced cytotoxicity was confirmed by siRNA-mediated silencing of this kinase. Using RNAi-based methods we also showed the importance of Bak-dependent mitochondrial apoptotic pathway in the combined anticancer action of SCH900776, cisplatin and TRAIL. The triple drug combination-induced cytotoxicity was partially enhanced by siRNA-mediated Mcl-1 silencing. Our findings suggest that targeting Chk1 may be used as an efficient strategy for sensitization of prostate cancer cells to killing action of platinum-based chemotherapeutic drugs and TRAIL.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"395-406"},"PeriodicalIF":2.4,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140058616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KCTD5 regulates Ikaros degradation induced by chemotherapeutic drug etoposide in hematological cells. KCTD5调节化疗药物依托泊苷在血液细胞中诱导的Ikaros降解。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-03-01 Print Date: 2024-05-27 DOI: 10.1515/hsz-2023-0333

Lan Ma, Changqing Yin, Yi Zhang, Jie Li, Liuzhi Shi, Tong Zhou, Xixi Huang, Yaqi Liu, Jiawei Cao, Guang Wu, Haihua Gu, Licai He

{"title":"KCTD5 regulates Ikaros degradation induced by chemotherapeutic drug etoposide in hematological cells.","authors":"Lan Ma, Changqing Yin, Yi Zhang, Jie Li, Liuzhi Shi, Tong Zhou, Xixi Huang, Yaqi Liu, Jiawei Cao, Guang Wu, Haihua Gu, Licai He","doi":"10.1515/hsz-2023-0333","DOIUrl":"10.1515/hsz-2023-0333","url":null,"abstract":"Therapy-related leukemia carries a poor prognosis, and leukemia after chemotherapy is a growing risk in clinic, whose mechanism is still not well understood. Ikaros transcription factor is an important regulator in hematopoietic cells development and differentiation. In the absence of Ikaros, lymphoid cell differentiation is blocked at an extremely early stage, and myeloid cell differentiation is also significantly affected. In this work, we showed that chemotherapeutic drug etoposide reduced the protein levels of several isoforms of Ikaros including IK1, IK2 and IK4, but not IK6 or IK7, by accelerating protein degradation, in leukemic cells. To investigate the molecular mechanism of Ikaros degradation induced by etoposide, immunoprecipitation coupled with LC-MS/MS analysis was conducted to identify changes in protein interaction with Ikaros before and after etoposide treatment, which uncovered KCTD5 protein. Our further study demonstrates that KCTD5 is the key stabilizing factor of Ikaros and chemotherapeutic drug etoposide induces Ikaros protein degradation through decreasing the interaction of Ikaros with KCTD5. These results suggest that etoposide may induce leukemic transformation by downregulating Ikaros via KCTD5, and our work may provide insights to attenuate the negative impact of chemotherapy on hematopoiesis.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"341-349"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cathepsin X deficiency alters the processing and localisation of cathepsin L and impairs cleavage of a nuclear cathepsin L substrate. 缺乏螯合蛋白酶 X 会改变螯合蛋白酶 L 的加工和定位，并影响螯合蛋白酶 L 核底物的裂解。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-02-28 Print Date: 2024-05-27 DOI: 10.1515/hsz-2023-0355

Bangyan Xu, Bethany M Anderson, Simon J Mountford, Philip E Thompson, Justine D Mintern, Laura E Edgington-Mitchell

{"title":"Cathepsin X deficiency alters the processing and localisation of cathepsin L and impairs cleavage of a nuclear cathepsin L substrate.","authors":"Bangyan Xu, Bethany M Anderson, Simon J Mountford, Philip E Thompson, Justine D Mintern, Laura E Edgington-Mitchell","doi":"10.1515/hsz-2023-0355","DOIUrl":"10.1515/hsz-2023-0355","url":null,"abstract":"Proteases function within sophisticated networks. Altering the activity of one protease can have sweeping effects on other proteases, leading to changes in their activity, structure, specificity, localisation, stability, and expression. Using a suite of chemical tools, we investigated the impact of cathepsin X, a lysosomal cysteine protease, on the activity and expression of other cysteine proteases and their inhibitors in dendritic cells. Among all proteases examined, cathepsin X gene deletion specifically altered cathepsin L levels; pro-cathepsin L and its single chain accumulated while the two-chain form was unchanged. This effect was recapitulated by chemical inhibition of cathepsin X, suggesting a dependence on its catalytic activity. We demonstrated that accumulation of pro- and single chain cathepsin L was not due to a lack of direct cleavage by cathepsin X or altered glycosylation, secretion, or mRNA expression but may result from changes in lysosomal oxidative stress or pH. In the absence of active cathepsin X, nuclear cathepsin L and cleavage of the known nuclear cathepsin L substrate, Lamin B1, were diminished. Thus, cathepsin X activity selectively regulates cathepsin L, which has the potential to impact the degree of cathepsin L proteolysis, the nature of substrates that it cleaves, and the location of cleavage.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"351-365"},"PeriodicalIF":2.4,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical evidence for conformational variants in the anti-viral and pro-metastatic protein IFITM1. 抗病毒和促转移蛋白 IFITM1 构象变异的生化证据。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-02-22 Print Date: 2024-05-27 DOI: 10.1515/hsz-2023-0327

Marta Nekulová, Marta Wyszkowska, Nela Friedlová, Lukáš Uhrík, Filip Zavadil Kokáš, Václav Hrabal, Lenka Hernychová, Bořivoj Vojtěšek, Ted R Hupp, Michał R Szymański

{"title":"Biochemical evidence for conformational variants in the anti-viral and pro-metastatic protein IFITM1.","authors":"Marta Nekulová, Marta Wyszkowska, Nela Friedlová, Lukáš Uhrík, Filip Zavadil Kokáš, Václav Hrabal, Lenka Hernychová, Bořivoj Vojtěšek, Ted R Hupp, Michał R Szymański","doi":"10.1515/hsz-2023-0327","DOIUrl":"10.1515/hsz-2023-0327","url":null,"abstract":"Interferon induced transmembrane proteins (IFITMs) play a dual role in the restriction of RNA viruses and in cancer progression, yet the mechanism of their action remains unknown. Currently, there is no data about the basic biochemical features or biophysical properties of the IFITM1 protein. In this work, we report on description and biochemical characterization of three conformational variants/oligomeric species of recombinant IFITM1 protein derived from an Escherichia coli expression system. The protein was extracted from the membrane fraction, affinity purified, and separated by size exclusion chromatography where two distinct oligomeric species were observed in addition to the expected monomer. These species remained stable upon re-chromatography and were designated as \"dimer\" and \"oligomer\" according to their estimated molecular weight. The dimer was found to be less stable compared to the oligomer using circular dichroism thermal denaturation and incubation with a reducing agent. A two-site ELISA and HDX mass spectrometry suggested the existence of structural motif within the N-terminal part of IFITM1 which might be significant in oligomer formation. Together, these data show the unusual propensity of recombinant IFITM1 to naturally assemble into very stable oligomeric species whose study might shed light on IFITM1 anti-viral and pro-oncogenic functions in cells.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"311-324"},"PeriodicalIF":2.4,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features. 牛超长CDR-H3衍生的旋钮副基团可产生强效的TNF-α中和作用，并能生成具有增强功能的新型阿达木单抗抗体结构。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-02-20 Print Date: 2024-07-26 DOI: 10.1515/hsz-2023-0370

Paul Arras, Jasmin Zimmermann, Britta Lipinski, Bernhard Valldorf, Andreas Evers, Desislava Elter, Simon Krah, Achim Doerner, Enrico Guarnera, Vanessa Siegmund, Harald Kolmar, Lukas Pekar, Stefan Zielonka

引用次数: 0

In-depth analysis of Gαs protein activity by probing different fluorescently labeled guanine nucleotides. 通过探测不同的荧光标记鸟嘌呤核苷酸，深入分析 Gαs 蛋白的活性。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-02-02 Print Date: 2024-05-27 DOI: 10.1515/hsz-2023-0321

Anna Pepanian, Paul Sommerfeld, Furkan Ayberk Binbay, Dietmar Fischer, Markus Pietsch, Diana Imhof

{"title":"In-depth analysis of Gαs protein activity by probing different fluorescently labeled guanine nucleotides.","authors":"Anna Pepanian, Paul Sommerfeld, Furkan Ayberk Binbay, Dietmar Fischer, Markus Pietsch, Diana Imhof","doi":"10.1515/hsz-2023-0321","DOIUrl":"10.1515/hsz-2023-0321","url":null,"abstract":"G proteins are interacting partners of G protein-coupled receptors (GPCRs) in eukaryotic cells. Upon G protein activation, the ability of the Gα subunit to exchange GDP for GTP determines the intracellular signal transduction. Although various studies have successfully shown that both Gαs and Gαi have an opposite effect on the intracellular cAMP production, with the latter being commonly described as \"more active\", the functional analysis of Gαs is a comparably more complicated matter. Additionally, the thorough investigation of the ubiquitously expressed variants of Gαs, Gαs(short) and Gαs(long), is still pending. Since the previous experimental evaluation of the activity and function of the Gαs isoforms is not consistent, the focus was laid on structural investigations to understand the GTPase activity. Herein, we examined recombinant human Gαs by applying an established methodological setup developed for Gαi characterization. The ability for GTP binding was evaluated with fluorescence and fluorescence anisotropy assays, whereas the intrinsic hydrolytic activity of the isoforms was determined by a GTPase assay. Among different nucleotide probes, BODIPY FL GTPγS exhibited the highest binding affinity towards the Gαs subunit. This work provides a deeper understanding of the Gαs subunit and provides novel information concerning the differences between the two protein variants.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"297-309"},"PeriodicalIF":2.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Better safe than sorry: dual targeting antibodies for cancer immunotherapy. 安全总比遗憾好：用于癌症免疫疗法的双靶向抗体。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2024-02-01 Print Date: 2024-07-26 DOI: 10.1515/hsz-2023-0329

Katrin Schoenfeld, Julia Harwardt, Harald Kolmar

引用次数: 0