Biological Chemistry最新文献_第5页

Sarcomere analysis in human cardiomyocytes by computing radial frequency spectra. 计算径向频谱的人心肌细胞肌瘤分析。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-12-29 DOI: 10.1515/hsz-2025-0173

Michael Habeck, Hafiza Nosheen Saleem, Daria Plota, Cleophas Cheruiyot, Tobias Kohl, Stephan E Lehnart, Stefan Jakobs, Antje Ebert

{"title":"Sarcomere analysis in human cardiomyocytes by computing radial frequency spectra.","authors":"Michael Habeck, Hafiza Nosheen Saleem, Daria Plota, Cleophas Cheruiyot, Tobias Kohl, Stephan E Lehnart, Stefan Jakobs, Antje Ebert","doi":"10.1515/hsz-2025-0173","DOIUrl":"https://doi.org/10.1515/hsz-2025-0173","url":null,"abstract":"In cardiomyocytes, the basic contractile unit are sarcomeres, which are organized in a regular manner facilitating their function. Here, we present a new computational approach to assess the functional properties of sarcomeres at the nanoscale level in human cardiac cells, induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). We combined our analysis to different types of high-resolution imaging data, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy-based imaging, as well as confocal microscopy data. We show that the radially averaged magnitude spectrum (RAMS) revealed sarcomere properties in a human cardiomyocyte model, iPSC-CMs, and compared our RAMS-based analysis to a real-space approach based on manually selected regions of interest. Moreover, we found the RAMS method suitable to quantify molecular differences of sarcomeres such as present in severe cardiac diseases, such as dilated cardiomyopathy (DCM). Defects in the sarcomere organization that occur in the presence of inherited DCM mutations in sarcomere proteins were efficiently recapitulated by our analysis. This new approach may facilitate streamlined analysis of molecular disease-specific phenotypic imaging data of cardiac cells, aiding our deeper understanding of the molecular basis of cardiac diseases.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights into Ras-catalyzed GTP hydrolysis: conformational dynamics, catalytic mechanisms, and emerging therapeutic strategies. ras催化GTP水解的机理：构象动力学、催化机制和新兴的治疗策略。

IF 2.5 4区生物学

Biological Chemistry Pub Date : 2025-12-22 Print Date: 2026-05-26 DOI: 10.1515/hsz-2025-0227

Carsten Kötting, Till Rudack, Klaus Gerwert

引用次数: 0

There and back again: a cell biologist's journey from organelles to molecules. 一个细胞生物学家从细胞器到分子的旅程。

IF 2.5 4区生物学

Biological Chemistry Pub Date : 2025-12-18 Print Date: 2025-12-17 DOI: 10.1515/hsz-2025-0185

Emma J Fenech, Yury S Bykov

{"title":"There and back again: a cell biologist's journey from organelles to molecules.","authors":"Emma J Fenech, Yury S Bykov","doi":"10.1515/hsz-2025-0185","DOIUrl":"10.1515/hsz-2025-0185","url":null,"abstract":"Eukaryotic life is defined by the presence of organelles. Organelles, in turn, were classically defined as specialized membrane-bound compartments composed of a unique set of macromolecules which support specific functions. Over the last few decades, a concerted effort into uncovering which components are present in each organelle has shaped our view of cell biology. However, despite some organelles already being visualized over 100 years ago, we are still discovering new organelle residents. Furthermore, our concept of both 'organelles' and 'compartmentalization' has evolved together with our deepening understanding in a number of fields. These include: organelle substructure and organization; the network of contact sites which interconnects all organelles; and membraneless organelles and phase-separated condensates. This review explores how image- and mass spectrometry-based methods can be used to understand the spectrum of where components are localized: from complexes, to subdomains, and whole organelles. The components we mainly focus on are proteins of the mitochondria and secretory pathway organelles.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"431-455"},"PeriodicalIF":2.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12755127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jack of all trades - the lipid droplet organization (LDO) proteins are multifunctional organelle surface receptors. 脂滴组织（LDO）蛋白是多功能细胞器表面受体。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-12-17 DOI: 10.1515/hsz-2025-0216

Beatriz Leite, Maria Bohnert

{"title":"Jack of all trades - the lipid droplet organization (LDO) proteins are multifunctional organelle surface receptors.","authors":"Beatriz Leite, Maria Bohnert","doi":"10.1515/hsz-2025-0216","DOIUrl":"10.1515/hsz-2025-0216","url":null,"abstract":"The literature on the lipid droplet organization (LDO) proteins Ldo16 and Ldo45 reads like a guided tour through the lipid droplet life cycle. Both yeast Ldo16/45 and their metazoan counterparts, the LDAF1/promethin proteins, were originally identified based on their connection to the lipodystrophy protein seipin, a key player in lipid droplet biogenesis. Mechanistic follow-up studies support a role of LDAF1/LDO as conserved integral component of the seipin lipid droplet biogenesis complex. However, at the same time, additional LDO functions beyond lipid droplet formation were identified in yeast. Together with Vac8, Ldo16/45 act as tethers for formation of vacuole lipid droplet (vCLIP) contact sites, structures that are crucial for lipid droplet breakdown via microautophagy during glucose starvation. Ldo45 additionally recruits the lipid transfer protein Pdr16 to vCLIP. Furthermore, Ldo16 was identified as a central player in the process of actomyosin-based lipid droplet motility, by acting as a receptor for the myosin adaptor protein Ldm1. Based on these findings, we suggest an overarching molecular role of the LDO proteins as multifunctional lipid droplet surface receptors that are optimized to coordinate the different aspects of the lipid droplet life cycle through an interplay with different effector proteins.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"477-485"},"PeriodicalIF":2.4,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145761931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging dimensions of mitochondrial specialization. 线粒体特化的新维度。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-12-10 Print Date: 2025-12-17 DOI: 10.1515/hsz-2025-0210

Tslil Ast

引用次数: 0

Highlight: organelles on and off the map: diversity, specialization and subdomains. 重点：细胞器在地图上和地图上：多样性，专门化和子域。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-12-01 Print Date: 2025-12-17 DOI: 10.1515/hsz-2025-0237

Yury S Bykov, Emma J Fenech, Blanche Schwappach

引用次数: 0

Recent advances in glycosome biogenesis and its implications for drug discovery. 糖体生物发生的最新进展及其对药物发现的意义。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-12-01 Print Date: 2025-12-17 DOI: 10.1515/hsz-2025-0183

Lisa Hohnen, Chethan K Krishna, Lewis Walker, Ralf Erdmann, Vishal C Kalel

引用次数: 0

Molecular mechanisms shaping the actin filament scaffold of dendritic filopodia. 树突丝状足肌动蛋白丝支架形成的分子机制。

IF 2.5 4区生物学

Biological Chemistry Pub Date : 2025-11-25 Print Date: 2026-07-24 DOI: 10.1515/hsz-2025-0181

Marco B Rust, Sharof Khudayberdiev

{"title":"Molecular mechanisms shaping the actin filament scaffold of dendritic filopodia.","authors":"Marco B Rust, Sharof Khudayberdiev","doi":"10.1515/hsz-2025-0181","DOIUrl":"10.1515/hsz-2025-0181","url":null,"abstract":"Dendritic spines are the postsynaptic compartment of most excitatory synapses in the vertebrate brain. Their morphology is defined by a complex actin scaffold consisting of branched and unbranched actin filaments (F-actin), which constitute the major structural component of dendritic spines. During brain development, dendritic spines arise from dendritic filopodia, motile finger-like dendritic protrusions, whose morphology is also defined by an actin scaffold. The organization of the actin scaffold as well as its dynamic behavior in both dendritic filopodia and dendritic spines requires the coordinated activity of actin binding proteins (ABP) that promote either assembly or disassembly of F-actin. Studies of the past two decades identified a number of ABP and upstream regulatory pathways that control the morphology of dendritic spines as well as their morphological changes associated with synaptic plasticity, the cellular basis for learning and memory. Instead, much less is known about actin regulatory mechanisms that control the formation and elongation of dendritic filopodia or the structural changes associated with their transition into dendritic spines. This review article highlights recent advances in the field by summarizing and discussing studies of the past few years that provided exciting novel insights into the molecular machinery that governs dendritic filopodia initiation and their maturation into dendritic spines.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"151-158"},"PeriodicalIF":2.5,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on VAP, a ubiquitous signpost for the ER. VAP的最新进展，急诊室无处不在的路标。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-11-21 Print Date: 2025-12-17 DOI: 10.1515/hsz-2025-0199

Tim P Levine

引用次数: 0

Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria. 基于抗菌肽和海藻酸钠的ph响应纳米复合物的配方用于靶向递送万古霉素抗耐药细菌。

IF 2.4 4区生物学

Biological Chemistry Pub Date : 2025-11-05 Print Date: 2025-10-27 DOI: 10.1515/hsz-2025-0142

Shourok Shahin, Calvin A Omolo, Eman Elhassan, Eman A Ismail, Sania Farukh, Jasoda Govender, Mbuso Faya, Thirumala Govender

{"title":"Formulation of pH-responsive nanoplexes based on an antimicrobial peptide and sodium alginate for targeted delivery of vancomycin against resistant bacteria.","authors":"Shourok Shahin, Calvin A Omolo, Eman Elhassan, Eman A Ismail, Sania Farukh, Jasoda Govender, Mbuso Faya, Thirumala Govender","doi":"10.1515/hsz-2025-0142","DOIUrl":"10.1515/hsz-2025-0142","url":null,"abstract":"The rapid spread of bacterial resistance to antibiotics necessitates the development of innovative strategies to enhance their efficacy. One promising approach is incorporating antimicrobial peptides (AMPs) to synergize antibiotics. Herein, we introduce pH-responsive nanoplexes of plant AMP and sodium alginate (Na-Alg) for the co-delivery of AMP and Vancomycin (VCM) against resistant bacteria. The optimal nanoplexes (VCM-Na-Alg/AMP) were characterized, revealing a particle size, polydispersity index, zeta potential, encapsulation efficiency, and loading capacity of 159.5 ± 1.150 nm, 0.149 ± 0.018, -23.1 ± 0.1 mV, 82.34 ± 0.07 %, and 24.03 ± 0.10 % w/w, respectively. The nanoplexes exhibited pH-dependent changes in size and accelerated VCM release at acidic pH. In vitro antibacterial studies demonstrated a 2-fold enhanced activity against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) and a 5-fold greater MRSA biofilm eradication, compared to bare VCM. Furthermore, the in vivo antibacterial activity evaluated on a mice model of MRSA systemic infection demonstrated that the nanoplexes reduced MRSA burden by 5-fold in kidneys and 4-fold in liver and blood. The nanoplexes also exhibited reduced inflammation and improved tissue integrity in the treated subjects. These findings present VCM-Na-Alg/AMP as a novel strategy to augment the efficacy of antibiotics against resistant bacteria.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"369-389"},"PeriodicalIF":2.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145443816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0