Biological Chemistry最新文献_第6页

Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer. 组织蛋白酶L介导的EGFR切割影响癌症细胞内信号通路。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-10-30 Print Date: 2024-04-25 DOI: 10.1515/hsz-2023-0213

Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović

{"title":"Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer.","authors":"Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović","doi":"10.1515/hsz-2023-0213","DOIUrl":"10.1515/hsz-2023-0213","url":null,"abstract":"Proteolytic activity in the tumour microenvironment is an important factor in cancer development since it can also affect intracellular signalling pathways via positive feedback loops that result in either increased tumour growth or resistance to anticancer mechanisms. In this study, we demonstrated extracellular cathepsin L-mediated cleavage of epidermal growth factor receptor (EGFR) and identified the cleavage site in the extracellular domain after R224. To further evaluate the relevance of this cleavage, we cloned and expressed a truncated version of EGFR, starting at G225, in HeLa cells. We confirmed the constitutive activation of the truncated protein in the absence of ligand binding and determined possible changes in intracellular signalling. Furthermore, we determined the effect of truncated EGFR protein expression on HeLa cell viability and response to the EGFR inhibitors, tyrosine kinase inhibitor (TKI) erlotinib and monoclonal antibody (mAb) cetuximab. Our data reveal the nuclear localization and phosphorylation of EGFR and signal trancducer and activator of transcription 3 (STAT3) in cells that express the truncated EGFR protein and suggest that these phenomena cause resistance to EGFR inhibitors.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"283-296"},"PeriodicalIF":2.9,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61560302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of yeast RNA polymerase I with special consideration of the lobe binding subunits. 酵母核糖核酸聚合酶I的特征，特别考虑了叶结合亚基。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-13 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0184

Katrin Schwank, Catharina Schmid, Tobias Fremter, Christoph Engel, Philipp Milkereit, Joachim Griesenbeck, Herbert Tschochner

引用次数: 0

Towards correlative archaeology of the human mind. 走向人类心灵的相关考古学。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-12 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0199

Lukasz Piszczek, Joanna Kaczanowska, Wulf Haubensak

引用次数: 0

The good or the bad: an overview of autoantibodies in traumatic spinal cord injury. 好的或坏的：创伤脊髓损伤中自身抗体的概述。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-03 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0252

Annika Guntermann, Katrin Marcus, Caroline May

引用次数: 0

Frontmatter 头版头条

4区生物学

Biological Chemistry Pub Date : 2023-10-01 DOI: 10.1515/hsz-2023-frontmatter11-12

引用次数: 0

RBPome identification in egg-cell like callus of Arabidopsis. 拟南芥卵细胞样愈伤组织中RBPome的鉴定。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-29 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0195

Liping Liu, Jakob Trendel, Guojing Jiang, Yanhui Liu, Astrid Bruckmann, Bernhard Küster, Stefanie Sprunck, Thomas Dresselhaus, Andrea Bleckmann

{"title":"RBPome identification in egg-cell like callus of Arabidopsis.","authors":"Liping Liu, Jakob Trendel, Guojing Jiang, Yanhui Liu, Astrid Bruckmann, Bernhard Küster, Stefanie Sprunck, Thomas Dresselhaus, Andrea Bleckmann","doi":"10.1515/hsz-2023-0195","DOIUrl":"10.1515/hsz-2023-0195","url":null,"abstract":"RNA binding proteins (RBPs) have multiple and essential roles in transcriptional and posttranscriptional regulation of gene expression in all living organisms. Their biochemical identification in the proteome of a given cell or tissue requires significant protein amounts, which limits studies in rare and highly specialized cells. As a consequence, we know almost nothing about the role(s) of RBPs in reproductive processes such as egg cell development, fertilization and early embryogenesis in flowering plants. To systematically identify the RBPome of egg cells in the model plant Arabidopsis, we performed RNA interactome capture (RIC) experiments using the egg cell-like RKD2-callus and were able to identify 728 proteins associated with poly(A+)-RNA. Transcripts for 97 % of identified proteins could be verified in the egg cell transcriptome. 46 % of identified proteins can be associated with the RNA life cycle. Proteins involved in mRNA binding, RNA processing and metabolism are highly enriched. Compared with the few available RBPome datasets of vegetative plant tissues, we identified 475 egg cell-enriched RBPs, which will now serve as a resource to study RBP function(s) during egg cell development, fertilization and early embryogenesis. First candidates were already identified showing an egg cell-specific expression pattern in ovules.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"1137-1149"},"PeriodicalIF":3.7,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1-RAGE axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte autophagy and apoptosis in diabetic mice. HMGB1-RAGE轴通过调节糖尿病小鼠的心肌细胞自噬和凋亡，促进心肌缺血/再灌注损伤。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-28 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0134

De-Wei He, De-Zhao Liu, Xiao-Zhi Luo, Chuan-Bin Chen, Chuang-Hong Lu, Na Na, Feng Huang

{"title":"HMGB1-RAGE axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte autophagy and apoptosis in diabetic mice.","authors":"De-Wei He, De-Zhao Liu, Xiao-Zhi Luo, Chuan-Bin Chen, Chuang-Hong Lu, Na Na, Feng Huang","doi":"10.1515/hsz-2023-0134","DOIUrl":"10.1515/hsz-2023-0134","url":null,"abstract":"Patients with acute myocardial infarction complicated with diabetes are more likely to develop myocardial ischemia/reperfusion (I/R) injury (MI/RI) during reperfusion therapy. Both HMGB1 and RAGE play important roles in MI/RI. However, the specific mechanisms of HMGB1 associated with RAGE are not fully clarified in diabetic MI/RI. This study aimed to investigate whether the HMGB1-RAGE axis induces diabetic MI/RI via regulating autophagy and apoptosis. A db/db mouse model of MI/RI was established, where anti-HMGB1 antibody and RAGE inhibitor (FPS-ZM1) were respectively injected after 10 min of reperfusion. The results showed that treatment with anti-HMGB1 significantly reduced the infarct size, serum LDH, and CK-MB level. Similar situations also occurred in mice administrated with FPS-ZM1, though the HMGB1 level was unchanged. Then, we found that treatment with anti-HMGB1 or FPS-ZM1 performed the same effects in suppressing the autophagy and apoptosis, as reflected by the results of lower LAMP2 and LC3B levels, increased Bcl-2 level, reduced BAX and caspase-3 levels. Moreover, the Pink1/Parkin levels were also inhibited at the same time. Collectively, this study indicates that the HMGB1-RAGE axis aggravated diabetic MI/RI via apoptosis and Pink1/Parkin mediated autophagy pathways, and inhibition of HMGB1 or RAGE contributes to alleviating those adverse situations.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"167-176"},"PeriodicalIF":2.9,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41109563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highlight: how to synthesize, assemble and regulate ribonucleoprotein-complexes. 推荐理由：如何合成、组装和调节核糖核蛋白复合物。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-27 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0301

Herbert Tschochner

引用次数: 0

Post-transcriptional gene silencing in a dynamic RNP world. 动态RNP世界中的转录后基因沉默。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-09-25 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0203

Simone Larivera, Julia Neumeier, Gunter Meister

引用次数: 0

Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation. 硝基油酸及其与伽马射线照射结合的细胞特异性抗癌作用。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-09-15 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0150

Tomas Perecko, Jana Pereckova, Zuzana Hoferova, Martin Falk

{"title":"Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation.","authors":"Tomas Perecko, Jana Pereckova, Zuzana Hoferova, Martin Falk","doi":"10.1515/hsz-2023-0150","DOIUrl":"10.1515/hsz-2023-0150","url":null,"abstract":"Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO2OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO2OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50 % survival fraction (DERIC50) was calculated for samples pre-treated with NO2OA followed by gamma irradiation. NO2OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO2OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO2OA prior irradiation showed a significantly increased DERIC50, demonstrating radiosensitizing effects. In conclusion, NO2OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"177-187"},"PeriodicalIF":2.9,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0