Biological Chemistry最新文献_第10页

Mechanism of hypoxia-induced exosome circ_0051799 regulating the progression of lung adenocarcinoma. 缺氧诱导外泌体circ_0051799调控肺腺癌进展的机制

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-05-11 Print Date: 2024-02-26 DOI: 10.1515/hsz-2023-0108

Shunping Zhu, Bihong Liao

{"title":"Mechanism of hypoxia-induced exosome circ_0051799 regulating the progression of lung adenocarcinoma.","authors":"Shunping Zhu, Bihong Liao","doi":"10.1515/hsz-2023-0108","DOIUrl":"10.1515/hsz-2023-0108","url":null,"abstract":"This study attempted to investigate the effect of circ_0051799 on the immune microenvironment of lung adenocarcinoma (LUAD) and the relationship between circ_0051799 and exosomes. The number and morphology of exosomes were verified by nanoparticle tracking, transmission electron microscopy and western blotting. CCK8, EdU, Transwell and flow cytometry were used to verify the regulatory role of exosomes and circ_0051799 on tumor progression. Dual luciferase reporting and RNA immunoprecipitation were used to verify the targeted regulatory relationship between circ_0051799, miR-214-3p and IGF2BP3. WB was used to verify the role of the JAK/STAT pathway in circ_0051799 regulation. Ectopic tumor grafts and in situ models were used to validate in vivo their role in regulating LUAD progression. Hypoxic environment could alter but does not alter its shape. Exosomes can participate in the regulation of macrophage polarization by circ_0051799. In vitro and in vivo assays had shown that circ_0051799 could affect the proliferation and metastasis of LUAD through targeting miR-214-3p mediated IGF2BP3 regulated JAK/STAT pathway. This study found that hypoxia can affect LUAD process by promoting the regulation of macrophage polarization by exosome circ_0051799.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":" ","pages":"143-160"},"PeriodicalIF":2.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9444203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein transport along the presequence pathway. 蛋白质沿序列前途径的转运。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-05-09 Print Date: 2023-07-26 DOI: 10.1515/hsz-2023-0133

Abhijith Makki, Peter Rehling

引用次数: 0

Autophagic and non-autophagic functions of the Saccharomyces cerevisiae PROPPINs Atg18, Atg21 and Hsv2. 酿酒酵母PROPPIN Atg18、Atg21和Hsv2的自噬和非自噬功能。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-05-05 Print Date: 2023-07-26 DOI: 10.1515/hsz-2023-0126

Lisa Marquardt, Michael Thumm

引用次数: 1

Frontmatter 头版头条

4区生物学

Biological Chemistry Pub Date : 2023-05-01 DOI: 10.1515/hsz-2023-frontmatter6

引用次数: 0

Determinants of synergistic cell-cell interactions in bacteria. 细菌中细胞-细胞协同作用的决定因素。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0303

Benedikt Pauli, Shiksha Ajmera, Christian Kost

{"title":"Determinants of synergistic cell-cell interactions in bacteria.","authors":"Benedikt Pauli, Shiksha Ajmera, Christian Kost","doi":"10.1515/hsz-2022-0303","DOIUrl":"https://doi.org/10.1515/hsz-2022-0303","url":null,"abstract":"Bacteria are ubiquitous and colonize virtually every conceivable habitat on earth. To achieve this, bacteria require different metabolites and biochemical capabilities. Rather than trying to produce all of the needed materials by themselves, bacteria have evolved a range of synergistic interactions, in which they exchange different commodities with other members of their local community. While it is widely acknowledged that synergistic interactions are key to the ecology of both individual bacteria and entire microbial communities, the factors determining their establishment remain poorly understood. Here we provide a comprehensive overview over our current knowledge on the determinants of positive cell-cell interactions among bacteria. Taking a holistic approach, we review the literature on the molecular mechanisms bacteria use to transfer commodities between bacterial cells and discuss to which extent these mechanisms favour or constrain the successful establishment of synergistic cell-cell interactions. In addition, we analyse how these different processes affect the specificity among interaction partners. By drawing together evidence from different disciplines that study the focal question on different levels of organisation, this work not only summarizes the state of the art in this exciting field of research, but also identifies new avenues for future research.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":"404 5","pages":"521-534"},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Drosophila collagens in specialised extracellular matrices. 果蝇胶原蛋白在专门的细胞外基质。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0297

Marcel Reinhardt, Maik Drechsler, Achim Paululat

{"title":"Drosophila collagens in specialised extracellular matrices.","authors":"Marcel Reinhardt, Maik Drechsler, Achim Paululat","doi":"10.1515/hsz-2022-0297","DOIUrl":"https://doi.org/10.1515/hsz-2022-0297","url":null,"abstract":"The basement membrane (BM) constitutes a specialised form of the extracellular matrix (ECM) and plays important roles in many biological processes, such as cell migration, organ and tissue integrity, cell polarity, and the formation of metastases. In metazoans, a canonical BM is formed by only a few conserved structural core proteins: Laminin, Collagen IV, Nidogen and Perlecan. Depending on the tissue's function and mechanical load, additional matrix proteins interact with, or are incorporated into the BM, resulting in tissue-specific mechanical properties, such as higher stiffness or elasticity, or special resistance to mechanical stress or harmful environmental conditions. In flies, the collagen IV-like protein Pericardin forms an integral constituent of matrices around the heart and tension sensors (chordotonal organs) of the peripheral nervous system. The function and integrity of both organ systems strongly relies on the appropriate establishment of a Pericardin (Prc) matrix and the function of its adapter protein-Lonely heart (Loh). In this review, we provide an overview of the four collagens present in flies, and will discuss our recent work on the formation and function of Pericardin-containing matrices, the role of the adapter protein Lonely heart and the necessity of specialised ECM molecules in tissue architecture and function.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":"404 5","pages":"535-550"},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9312886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular insights into endolysosomal microcompartment formation and maintenance. 内溶酶体微室形成和维持的分子机制。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0294

Daniel Kümmel, Eric Herrmann, Lars Langemeyer, Christian Ungermann

{"title":"Molecular insights into endolysosomal microcompartment formation and maintenance.","authors":"Daniel Kümmel, Eric Herrmann, Lars Langemeyer, Christian Ungermann","doi":"10.1515/hsz-2022-0294","DOIUrl":"https://doi.org/10.1515/hsz-2022-0294","url":null,"abstract":"The endolysosomal system of eukaryotic cells has a key role in the homeostasis of the plasma membrane, in signaling and nutrient uptake, and is abused by viruses and pathogens for entry. Endocytosis of plasma membrane proteins results in vesicles, which fuse with the early endosome. If destined for lysosomal degradation, these proteins are packaged into intraluminal vesicles, converting an early endosome to a late endosome, which finally fuses with the lysosome. Each of these organelles has a unique membrane surface composition, which can form segmented membrane microcompartments by membrane contact sites or fission proteins. Furthermore, these organelles are in continuous exchange due to fission and fusion events. The underlying machinery, which maintains organelle identity along the pathway, is regulated by signaling processes. Here, we will focus on the Rab5 and Rab7 GTPases of early and late endosomes. As molecular switches, Rabs depend on activating guanine nucleotide exchange factors (GEFs). Over the last years, we characterized the Rab7 GEF, the Mon1-Ccz1 (MC1) complex, and key Rab7 effectors, the HOPS complex and retromer. Structural and functional analyses of these complexes lead to a molecular understanding of their function in the context of organelle biogenesis.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":"404 5","pages":"441-454"},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Membrane damage and repair: a thin line between life and death. 膜损伤与修复:生死一线。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0321

Caroline Barisch, Joost C M Holthuis, Katia Cosentino

{"title":"Membrane damage and repair: a thin line between life and death.","authors":"Caroline Barisch, Joost C M Holthuis, Katia Cosentino","doi":"10.1515/hsz-2022-0321","DOIUrl":"https://doi.org/10.1515/hsz-2022-0321","url":null,"abstract":"Bilayered membranes separate cells from their surroundings and form boundaries between intracellular organelles and the cytosol. Gated transport of solutes across membranes enables cells to establish vital ion gradients and a sophisticated metabolic network. However, an advanced compartmentalization of biochemical reactions makes cells also particularly vulnerable to membrane damage inflicted by pathogens, chemicals, inflammatory responses or mechanical stress. To avoid potentially lethal consequences of membrane injuries, cells continuously monitor the structural integrity of their membranes and readily activate appropriate pathways to plug, patch, engulf or shed the damaged membrane area. Here, we review recent insights into the cellular mechanisms that underly an effective maintenance of membrane integrity. We discuss how cells respond to membrane lesions caused by bacterial toxins and endogenous pore-forming proteins, with a primary focus on the intimate crosstalk between membrane proteins and lipids during wound formation, detection and elimination. We also discuss how a delicate balance between membrane damage and repair determines cell fate upon bacterial infection or activation of pro-inflammatory cell death pathways.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":"404 5","pages":"467-490"},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Neprilysin 4: an essential peptidase with multifaceted physiological relevance. Neprilysin 4:一种重要的多肽酶，具有多方面的生理相关性。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0286

Annika Buhr, Ronja Schiemann, Heiko Meyer

引用次数: 1

Highlight: on the past and the future of cellular microcompartments. 重点:关于细胞微室的过去和未来。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2023-0153

Milos Galic, Christian Ungermann, Katia Cosentino

引用次数: 0