Biological Chemistry最新文献_第10页

Molecular insights into endolysosomal microcompartment formation and maintenance. 内溶酶体微室形成和维持的分子机制。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0294

Daniel Kümmel, Eric Herrmann, Lars Langemeyer, Christian Ungermann

{"title":"Molecular insights into endolysosomal microcompartment formation and maintenance.","authors":"Daniel Kümmel, Eric Herrmann, Lars Langemeyer, Christian Ungermann","doi":"10.1515/hsz-2022-0294","DOIUrl":"https://doi.org/10.1515/hsz-2022-0294","url":null,"abstract":"The endolysosomal system of eukaryotic cells has a key role in the homeostasis of the plasma membrane, in signaling and nutrient uptake, and is abused by viruses and pathogens for entry. Endocytosis of plasma membrane proteins results in vesicles, which fuse with the early endosome. If destined for lysosomal degradation, these proteins are packaged into intraluminal vesicles, converting an early endosome to a late endosome, which finally fuses with the lysosome. Each of these organelles has a unique membrane surface composition, which can form segmented membrane microcompartments by membrane contact sites or fission proteins. Furthermore, these organelles are in continuous exchange due to fission and fusion events. The underlying machinery, which maintains organelle identity along the pathway, is regulated by signaling processes. Here, we will focus on the Rab5 and Rab7 GTPases of early and late endosomes. As molecular switches, Rabs depend on activating guanine nucleotide exchange factors (GEFs). Over the last years, we characterized the Rab7 GEF, the Mon1-Ccz1 (MC1) complex, and key Rab7 effectors, the HOPS complex and retromer. Structural and functional analyses of these complexes lead to a molecular understanding of their function in the context of organelle biogenesis.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Membrane damage and repair: a thin line between life and death. 膜损伤与修复:生死一线。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0321

Caroline Barisch, Joost C M Holthuis, Katia Cosentino

{"title":"Membrane damage and repair: a thin line between life and death.","authors":"Caroline Barisch, Joost C M Holthuis, Katia Cosentino","doi":"10.1515/hsz-2022-0321","DOIUrl":"https://doi.org/10.1515/hsz-2022-0321","url":null,"abstract":"Bilayered membranes separate cells from their surroundings and form boundaries between intracellular organelles and the cytosol. Gated transport of solutes across membranes enables cells to establish vital ion gradients and a sophisticated metabolic network. However, an advanced compartmentalization of biochemical reactions makes cells also particularly vulnerable to membrane damage inflicted by pathogens, chemicals, inflammatory responses or mechanical stress. To avoid potentially lethal consequences of membrane injuries, cells continuously monitor the structural integrity of their membranes and readily activate appropriate pathways to plug, patch, engulf or shed the damaged membrane area. Here, we review recent insights into the cellular mechanisms that underly an effective maintenance of membrane integrity. We discuss how cells respond to membrane lesions caused by bacterial toxins and endogenous pore-forming proteins, with a primary focus on the intimate crosstalk between membrane proteins and lipids during wound formation, detection and elimination. We also discuss how a delicate balance between membrane damage and repair determines cell fate upon bacterial infection or activation of pro-inflammatory cell death pathways.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9317876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Determinants of synergistic cell-cell interactions in bacteria. 细菌中细胞-细胞协同作用的决定因素。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0303

Benedikt Pauli, Shiksha Ajmera, Christian Kost

{"title":"Determinants of synergistic cell-cell interactions in bacteria.","authors":"Benedikt Pauli, Shiksha Ajmera, Christian Kost","doi":"10.1515/hsz-2022-0303","DOIUrl":"https://doi.org/10.1515/hsz-2022-0303","url":null,"abstract":"Bacteria are ubiquitous and colonize virtually every conceivable habitat on earth. To achieve this, bacteria require different metabolites and biochemical capabilities. Rather than trying to produce all of the needed materials by themselves, bacteria have evolved a range of synergistic interactions, in which they exchange different commodities with other members of their local community. While it is widely acknowledged that synergistic interactions are key to the ecology of both individual bacteria and entire microbial communities, the factors determining their establishment remain poorly understood. Here we provide a comprehensive overview over our current knowledge on the determinants of positive cell-cell interactions among bacteria. Taking a holistic approach, we review the literature on the molecular mechanisms bacteria use to transfer commodities between bacterial cells and discuss to which extent these mechanisms favour or constrain the successful establishment of synergistic cell-cell interactions. In addition, we analyse how these different processes affect the specificity among interaction partners. By drawing together evidence from different disciplines that study the focal question on different levels of organisation, this work not only summarizes the state of the art in this exciting field of research, but also identifies new avenues for future research.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Neprilysin 4: an essential peptidase with multifaceted physiological relevance. Neprilysin 4:一种重要的多肽酶，具有多方面的生理相关性。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0286

Annika Buhr, Ronja Schiemann, Heiko Meyer

引用次数: 1

Highlight: on the past and the future of cellular microcompartments. 重点:关于细胞微室的过去和未来。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2023-0153

Milos Galic, Christian Ungermann, Katia Cosentino

引用次数: 0

Computational resolution in single molecule localization - impact of noise level and emitter density. 单分子定位的计算分辨率——噪声水平和发射器密度的影响。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0301

Mathias Hockmann, Stefan Kunis, Rainer Kurre

{"title":"Computational resolution in single molecule localization - impact of noise level and emitter density.","authors":"Mathias Hockmann, Stefan Kunis, Rainer Kurre","doi":"10.1515/hsz-2022-0301","DOIUrl":"https://doi.org/10.1515/hsz-2022-0301","url":null,"abstract":"Classical fluorescence microscopy is a powerful technique to image biological specimen under close-to-native conditions, but light diffraction limits its optical resolution to 200-300 nm-two orders of magnitude worse than the size of biomolecules. Assuming single fluorescent emitters, the final image of the optical system can be described by a convolution with the point spread function (PSF) smearing out details below the size of the PSF. In mathematical terms, fluorescence microscopy produces bandlimited space-continuous images that can be recovered from their spatial samples under the conditions of the classical Shannon-Nyquist theorem. During the past two decades, several single molecule localization techniques have been established and these allow for the determination of molecular positions with sub-pixel accuracy. Without noise, single emitter positions can be recovered precisely - no matter how close they are. We review recent work on the computational resolution limit with a sharp phase transition between two scenarios: 1) where emitters are well-separated with respect to the bandlimit and can be recovered up to the noise level and 2) closely distributed emitters which results in a strong noise amplification in the worst case. We close by discussing additional pitfalls using single molecule localization techniques based on structured illumination.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9367349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The readily retrievable pool of synaptic vesicles. 突触囊泡易于恢复的池。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0298

Sai Krishnan, Jürgen Klingauf

引用次数: 1

Nuclear redox processes in land plant development and stress adaptation. 陆地植物发育和逆境适应中的核氧化还原过程。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-04-25 DOI: 10.1515/hsz-2022-0288

Sabine Zachgo

引用次数: 3

The role of lysosomes in lipid homeostasis. 溶酶体在脂质稳态中的作用。