外源核核糖核蛋白D样控制内皮细胞功能。

IF 2.9 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biological Chemistry Pub Date : 2023-11-10 Print Date: 2024-04-25 DOI:10.1515/hsz-2023-0254
Sandra Fischer, Chiara Lichtenthaeler, Anastasiya Stepanenko, Florian Heyl, Daniel Maticzka, Katrin Kemmerer, Melina Klostermann, Rolf Backofen, Kathi Zarnack, Julia E Weigand
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引用次数: 0

摘要

HnRNP是一种普遍表达的RNA结合蛋白,严格控制转录后基因调控。因此,hnRNP网络对细胞稳态至关重要,其失调与癌症和其他疾病有关。然而,人们对hnRNPs在非癌细胞系统中的生理功能知之甚少。我们分析了HNRNPDL在内皮细胞功能中的重要性。HNRNPDL的敲除导致球体的增殖、迁移和发芽受损。转录组分析确定细胞周期蛋白D1(CCND1)和原肌球蛋白4(TPM4)是HNRNPDL的靶标,反映了敲除后的表型变化。我们的发现强调了HNRNPDL对内皮细胞生理过程稳态的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heterogenous nuclear ribonucleoprotein D-like controls endothelial cell functions.

HnRNPs are ubiquitously expressed RNA-binding proteins, tightly controlling posttranscriptional gene regulation. Consequently, hnRNP networks are essential for cellular homeostasis and their dysregulation is associated with cancer and other diseases. However, the physiological function of hnRNPs in non-cancerous cell systems are poorly understood. We analyzed the importance of HNRNPDL in endothelial cell functions. Knockdown of HNRNPDL led to impaired proliferation, migration and sprouting of spheroids. Transcriptome analysis identified cyclin D1 (CCND1) and tropomyosin 4 (TPM4) as targets of HNRNPDL, reflecting the phenotypic changes after knockdown. Our findings underline the importance of HNRNPDL for the homeostasis of physiological processes in endothelial cells.

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来源期刊
Biological Chemistry
Biological Chemistry 生物-生化与分子生物学
CiteScore
7.20
自引率
0.00%
发文量
63
审稿时长
4-8 weeks
期刊介绍: Biological Chemistry keeps you up-to-date with all new developments in the molecular life sciences. In addition to original research reports, authoritative reviews written by leading researchers in the field keep you informed about the latest advances in the molecular life sciences. Rapid, yet rigorous reviewing ensures fast access to recent research results of exceptional significance in the biological sciences. Papers are published in a "Just Accepted" format within approx.72 hours of acceptance.
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