Biological Chemistry最新文献_第3页

Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features. 牛超长CDR-H3衍生的旋钮副基团可产生强效的TNF-α中和作用，并能生成具有增强功能的新型阿达木单抗抗体结构。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2024-02-20 Print Date: 2024-07-26 DOI: 10.1515/hsz-2023-0370

Paul Arras, Jasmin Zimmermann, Britta Lipinski, Bernhard Valldorf, Andreas Evers, Desislava Elter, Simon Krah, Achim Doerner, Enrico Guarnera, Vanessa Siegmund, Harald Kolmar, Lukas Pekar, Stefan Zielonka

引用次数: 0

Celastrol inhibits angiogenesis and the biological processes of MDA-MB-231 cells via the DEGS1/S1P signaling pathway Celastrol 通过 DEGS1/S1P 信号通路抑制血管生成和 MDA-MB-231 细胞的生物过程

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-12-11 DOI: 10.1515/hsz-2023-0324

Lulu Jia, Shengnan Zhu, Mingfei Zhu, Rongrong Nie, Lingyue Huang, Siyuan Xu, Yuqin Luo, Huazhen Su, Shaoyuan Huang, Qinyou Tan

{"title":"Celastrol inhibits angiogenesis and the biological processes of MDA-MB-231 cells via the DEGS1/S1P signaling pathway","authors":"Lulu Jia, Shengnan Zhu, Mingfei Zhu, Rongrong Nie, Lingyue Huang, Siyuan Xu, Yuqin Luo, Huazhen Su, Shaoyuan Huang, Qinyou Tan","doi":"10.1515/hsz-2023-0324","DOIUrl":"https://doi.org/10.1515/hsz-2023-0324","url":null,"abstract":"Celastrol (Cel) shows potent antitumor activity in various experimental models. This study examined the relationship between Cel’s antivascular and antitumor effects and sphingolipids. CCK-8 assay, transwell assay, Matrigel, PCR-array/RT-PCR/western blotting/immunohistochemistry assay, ELISA and HE staining were used to detect cell proliferation, migration and invasion, adhesion and angiogenesis, mRNA and protein expression, S1P production and tumor morphology. The results showed that Cel could inhibit proliferation, migration or invasion, adhesion and angiogenesis of human umbilical vein endothelial cells (HUVECs) and MDA-MB-231 cells by downregulating the expression of degenerative spermatocyte homolog 1 (DEGS1). Transfection experiments showed that downregulation of DEGS1 inhibited the above processes and sphingosine-1-phosphate (S1P) production of HUVECs and MDA-MB-231 cells, while upregulation of DEGS1 had the opposite effects. Coculture experiments showed that HUVECs could promote proliferation, migration and invasion of MDA-MB-231 cells through S1P/sphingosine-1-phosphate receptor (S1PR) signaling pathway, while Cel inhibited these processes in MDA-MB-231 cells induced by HUVECs. Animal experiments showed that Cel could inhibit tumor growth in nude mice. Western blotting, immunohistochemistry and ELISA assay showed that Cel downregulated the expression of DEGS1, CD146, S1PR1-3 and S1P production. These data confirm that DEGS1/S1P signaling pathway may be related to the antivascular and antitumor effects of cel.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138572049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control. 亮点:神经科学的视野-类器官，光遗传学和远程控制。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-11-27 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0343

Rolf Heumann

引用次数: 0

Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy. 利用扫描离子电导和四倍扩增显微镜观察少突胶质祖细胞生长锥的膜表面和细胞骨架。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-11-13 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0217

Annika Haak, Heiko M Lesslich, Irmgard D Dietzel

{"title":"Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy.","authors":"Annika Haak, Heiko M Lesslich, Irmgard D Dietzel","doi":"10.1515/hsz-2023-0217","DOIUrl":"10.1515/hsz-2023-0217","url":null,"abstract":"Growth cones of oligodendrocyte progenitor cells (OPCs) are challenging to investigate with conventional light microscopy due to their small size. Especially substructures such as filopodia, lamellipodia and their underlying cytoskeleton are difficult to resolve with diffraction limited microscopy. Light microscopy techniques, which surpass the diffraction limit such as stimulated emission depletion microscopy, often require expensive setups and specially trained personnel rendering them inaccessible to smaller research groups. Lately, the invention of expansion microscopy (ExM) has enabled super-resolution imaging with any light microscope without the need for additional equipment. Apart from the necessary resolution, investigating OPC growth cones comes with another challenge: Imaging the topography of membranes, especially label- and contact-free, is only possible with very few microscopy techniques one of them being scanning ion conductance microscopy (SICM). We here present a new imaging workflow combining SICM and ExM, which enables the visualization of OPC growth cone nanostructures. We correlated SICM recordings and ExM images of OPC growth cones captured with a conventional widefield microscope. This enabled the visualization of the growth cones' membrane topography as well as their underlying actin and tubulin cytoskeleton.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89716832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogenous nuclear ribonucleoprotein D-like controls endothelial cell functions. 外源核核糖核蛋白D样控制内皮细胞功能。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-11-10 Print Date: 2024-04-25 DOI: 10.1515/hsz-2023-0254

Sandra Fischer, Chiara Lichtenthaeler, Anastasiya Stepanenko, Florian Heyl, Daniel Maticzka, Katrin Kemmerer, Melina Klostermann, Rolf Backofen, Kathi Zarnack, Julia E Weigand

引用次数: 0

Methionine restriction attenuates the migration and invasion of gastric cancer cells by inhibiting nuclear p65 translocation through TRIM47. 甲硫氨酸限制通过TRIM47抑制核p65易位来减弱癌症细胞的迁移和侵袭。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-11-10 Print Date: 2024-04-25 DOI: 10.1515/hsz-2023-0292

Lin Xin, Yi-Wu Yuan, Chen-Xi Liu, Jie Sheng, Qi Zhou, Zhi-Yang Liu, Zhen-Qi Yue, Fei Zeng

{"title":"Methionine restriction attenuates the migration and invasion of gastric cancer cells by inhibiting nuclear p65 translocation through TRIM47.","authors":"Lin Xin, Yi-Wu Yuan, Chen-Xi Liu, Jie Sheng, Qi Zhou, Zhi-Yang Liu, Zhen-Qi Yue, Fei Zeng","doi":"10.1515/hsz-2023-0292","DOIUrl":"10.1515/hsz-2023-0292","url":null,"abstract":"The prevention and treatment of gastric cancer has been the focus and difficulty of medical research. We aimed to explore the mechanism of inhibiting migration and invasion of gastric cancer cells by methionine restriction (MR). The human gastric cancer cell lines AGS and MKN45 cultured with complete medium (CM) or medium without methionine were used for in vitro experiments. MKN45 cells were injected tail vein into BALB/c nude mice and then fed with normal diet or methionine diet for in vivo experiments. MR treatment decreased cell migration and invasion, increased E-cadherin expression, decreased N-cadherin and p-p65 expressions, and inhibited nuclear p65 translocation of AGS and MKN45 cells when compared with CM group. MR treatment increased IκBα protein expression and protein stability, and decreased IκBα protein ubiquitination level and TRIM47 expression. TRIM47 interacted with IκBα protein, and overexpression of TRIM47 reversed the regulatory effects of MR. TRIM47 promoted lung metastasis formation and partially attenuated the effect of MR on metastasis formation in vivo compared to normal diet group mice. MR reduces TRIM47 expression, leads to the degradation of IκBα, and then inhibits the translocation of nuclear p65 and the migration and invasion of gastric cancer cells.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72013332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-derived growth factor-stimulated pulmonary artery smooth muscle cells regulate pulmonary artery endothelial cell dysfunction through extracellular vesicle miR-409-5p. 血小板衍生生长因子刺激的肺动脉平滑肌细胞通过细胞外小泡miR-409-5p调节肺动脉内皮细胞功能障碍。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-10-31 Print Date: 2024-03-25 DOI: 10.1515/hsz-2023-0222

Jeongyeon Heo, Hara Kang

{"title":"Platelet-derived growth factor-stimulated pulmonary artery smooth muscle cells regulate pulmonary artery endothelial cell dysfunction through extracellular vesicle miR-409-5p.","authors":"Jeongyeon Heo, Hara Kang","doi":"10.1515/hsz-2023-0222","DOIUrl":"10.1515/hsz-2023-0222","url":null,"abstract":"Platelet-derived growth factor (PDGF)-induced changes in vascular smooth muscle cells (VSMCs) stimulate vascular remodeling, resulting in vascular diseases such as pulmonary arterial hypertension. VSMCs communicate with endothelial cells through extracellular vesicles (EVs) carrying cargos, including microRNAs. To understand the molecular mechanisms through which PDGF-stimulated pulmonary artery smooth muscle cells (PASMCs) interact with pulmonary artery endothelial cells (PAECs) under pathological conditions, we investigated the crosstalk between PASMCs and PAECs via extracellular vesicle miR-409-5p under PDGF stimulation. miR-409-5p expression was upregulated in PASMCs upon PDGF signaling, and it was released into EVs. The elevated expression of miR-409-5p was transported to PAECs and led to their impaired function, including reduced NO release, which consequentially resulted in enhanced PASMC proliferation. We propose that the positive regulatory loop of PASMC-extracellular vesicle miR-409-5p-PAEC is a potential mechanism underlying the proliferation of PASMCs under PDGF stimulation. Therefore, miR-409-5p may be a novel therapeutic target for the treatment of vascular diseases, including pulmonary arterial hypertension.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71410451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer. 组织蛋白酶L介导的EGFR切割影响癌症细胞内信号通路。

IF 2.9 4区生物学

Biological Chemistry Pub Date : 2023-10-30 Print Date: 2024-04-25 DOI: 10.1515/hsz-2023-0213

Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović

{"title":"Cathepsin L-mediated EGFR cleavage affects intracellular signalling pathways in cancer.","authors":"Marija Grozdanić, Barbara Sobotič, Monika Biasizzo, Tilen Sever, Robert Vidmar, Matej Vizovišek, Boris Turk, Marko Fonović","doi":"10.1515/hsz-2023-0213","DOIUrl":"10.1515/hsz-2023-0213","url":null,"abstract":"Proteolytic activity in the tumour microenvironment is an important factor in cancer development since it can also affect intracellular signalling pathways via positive feedback loops that result in either increased tumour growth or resistance to anticancer mechanisms. In this study, we demonstrated extracellular cathepsin L-mediated cleavage of epidermal growth factor receptor (EGFR) and identified the cleavage site in the extracellular domain after R224. To further evaluate the relevance of this cleavage, we cloned and expressed a truncated version of EGFR, starting at G225, in HeLa cells. We confirmed the constitutive activation of the truncated protein in the absence of ligand binding and determined possible changes in intracellular signalling. Furthermore, we determined the effect of truncated EGFR protein expression on HeLa cell viability and response to the EGFR inhibitors, tyrosine kinase inhibitor (TKI) erlotinib and monoclonal antibody (mAb) cetuximab. Our data reveal the nuclear localization and phosphorylation of EGFR and signal trancducer and activator of transcription 3 (STAT3) in cells that express the truncated EGFR protein and suggest that these phenomena cause resistance to EGFR inhibitors.","PeriodicalId":8885,"journal":{"name":"Biological Chemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61560302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of yeast RNA polymerase I with special consideration of the lobe binding subunits. 酵母核糖核酸聚合酶I的特征，特别考虑了叶结合亚基。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-13 Print Date: 2023-10-26 DOI: 10.1515/hsz-2023-0184

Katrin Schwank, Catharina Schmid, Tobias Fremter, Christoph Engel, Philipp Milkereit, Joachim Griesenbeck, Herbert Tschochner

引用次数: 0

Towards correlative archaeology of the human mind. 走向人类心灵的相关考古学。

IF 3.7 4区生物学

Biological Chemistry Pub Date : 2023-10-12 Print Date: 2024-01-29 DOI: 10.1515/hsz-2023-0199

Lukasz Piszczek, Joanna Kaczanowska, Wulf Haubensak

引用次数: 0