洞穴蛋白结构排列及其局部脂质环境的见解。

IF 2.9 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Esther Ocket, Claudia Matthaeus
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引用次数: 0

摘要

腔隙是脂肪细胞、内皮细胞或成纤维细胞中发现的 50-80 nm 大小的质膜内陷。它们参与内吞、脂质摄取和细胞脂质代谢的调节,以及感知和适应质膜张力的变化。洞穴小体的特点是其独特的脂质成分和由洞穴素和洞穴蛋白组成的特异性蛋白衣壳。最近,研究人员获得了洞穴内主要蛋白洞穴素1的详细结构信息,显示其形成了一个圆盘状的11聚合蛋白复合物。此外,还揭示了洞穴素无序区在质膜上生成洞穴素三聚体和洞穴小体的过程中的重要性。因此,最终可以从结构上阐明洞穴外皮的组装。在此,我们回顾了洞穴泡结构生物学在洞穴泡外膜形成和洞穴泡曲率生成方面的最新进展。其次,我们讨论了洞穴泡弯曲和形成所必需的特定脂质种类的重要性。过去几年的研究表明,特定的鞘脂类、胆固醇和脂肪酸可在洞穴内陷中积聚,并可能驱动洞穴内陷的内吞作用。我们总结了该领域的最新研究,并强调了未来的研究方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insights in caveolae protein structure arrangements and their local lipid environment.

Caveolae are 50-80 nm sized plasma membrane invaginations found in adipocytes, endothelial cells or fibroblasts. They are involved in endocytosis, lipid uptake and the regulation of the cellular lipid metabolism as well as sensing and adapting to changes in plasma membrane tension. Caveolae are characterized by their unique lipid composition and their specific protein coat consisting of caveolin and cavin proteins. Recently, detailed structural information was obtained for the major caveolae protein caveolin1 showing the formation of a disc-like 11-mer protein complex. Furthermore, the importance of the cavin disordered regions in the generation of cavin trimers and caveolae at the plasma membrane were revealed. Thus, finally, structural insights about the assembly of the caveolar coat can be elucidated. Here, we review recent developments in caveolae structural biology with regard to caveolae coat formation and caveolae curvature generation. Secondly, we discuss the importance of specific lipid species necessary for caveolae curvature and formation. In the last years, it was shown that specifically sphingolipids, cholesterol and fatty acids can accumulate in caveolae invaginations and may drive caveolae endocytosis. Throughout, we summarize recent studies in the field and highlight future research directions.

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来源期刊
Biological Chemistry
Biological Chemistry 生物-生化与分子生物学
CiteScore
7.20
自引率
0.00%
发文量
63
审稿时长
4-8 weeks
期刊介绍: Biological Chemistry keeps you up-to-date with all new developments in the molecular life sciences. In addition to original research reports, authoritative reviews written by leading researchers in the field keep you informed about the latest advances in the molecular life sciences. Rapid, yet rigorous reviewing ensures fast access to recent research results of exceptional significance in the biological sciences. Papers are published in a "Just Accepted" format within approx.72 hours of acceptance.
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