Bioscience of Microbiota, Food and Health最新文献_第4页

Dietary phytochemicals, gut microbiota composition, and health outcomes in human and animal models. 人类和动物模型中的膳食植物化学物质、肠道微生物群组成和健康结果。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2022-078

Seyedeh Nooshan Mirmohammadali, Sara K Rosenkranz

{"title":"Dietary phytochemicals, gut microbiota composition, and health outcomes in human and animal models.","authors":"Seyedeh Nooshan Mirmohammadali, Sara K Rosenkranz","doi":"10.12938/bmfh.2022-078","DOIUrl":"https://doi.org/10.12938/bmfh.2022-078","url":null,"abstract":"The role of the composition of the gut microbiota on human health is not well understood. However, during the past decade, an increased emphasis has been placed on the influence of the impact of nutrition on the composition of gut microbiota and how the gut microbiota affects human health. The current review focuses on the role of some of the most studied phytochemicals on the composition of the gut microbiota. First, the review highlights the state of the research evidence regarding dietary phytochemical consumption and gut microbiota composition, including the influence of phytochemicals such as polyphenols, glucosinolates, flavonoids, and sterols that are present in vegetables, nuts, beans, and other foods. Second, the review identifies changes in health outcomes with altered gut microbiota composition, in both animal and human model studies. Third, the review highlights research that includes both associations between dietary phytochemical consumption and gut microbiota composition, and associations between the gut microbiota composition and health outcomes, in order to elucidate the role of the gut microbiota in the relationship between dietary phytochemical consumption and health outcomes in humans and animals. The current review indicated that phytochemicals can beneficially alter gut microbiota composition and decrease the risk for some diseases, such as cancers, and improve some cardiovascular and metabolic risk biomarkers. There is an urgent demand for high-quality studies that determine the relationships between the consumption of phytochemicals and health outcomes, examining gut microbiota as a moderator or mediator.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/97/bmfh-42-152.PMC10315191.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

High productivity of immunostimulatory membrane vesicles of Limosilactobacillus antri using glycine 抗利乳酸菌免疫刺激膜泡高产率的研究用甘氨酸

4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2023-029

Shino YAMASAKI-YASHIKI, Yu SAKAMOTO, Keiko NISHIMURA, Azusa SAIKA, Takeshi ITO, Jun KUNISAWA, Yoshio KATAKURA

{"title":"High productivity of immunostimulatory membrane vesicles of Limosilactobacillus antri using glycine","authors":"Shino YAMASAKI-YASHIKI, Yu SAKAMOTO, Keiko NISHIMURA, Azusa SAIKA, Takeshi ITO, Jun KUNISAWA, Yoshio KATAKURA","doi":"10.12938/bmfh.2023-029","DOIUrl":"https://doi.org/10.12938/bmfh.2023-029","url":null,"abstract":"Nanosized membrane vesicles (MVs) released by bacteria play important roles in both bacteria–bacteria and bacteria–host interactions. Some gram-positive lactic acid bacteria produce MVs exhibiting immunoregulatory activity in the host. We found that both bacterial cells and MVs of Limosilactobacillus antri JCM 15950, isolated from the human stomach mucosa, enhance immunoglobulin A production by murine Peyer’s patch cells. However, the thick cell walls of gram-positive bacteria resulted in low MV production, limiting experiments and applications using MVs. In this study, we evaluated the effects of glycine, which inhibits cell wall synthesis, on the immunostimulatory MV productivity of L. antri. Glycine inhibited bacterial growth while increasing MV production, with 20 g/L glycine increasing MV production approximately 12-fold. Glycine was most effective at increasing MV production when added in the early exponential phase, which indicated that cell division in the presence of glycine increased MV production. Finally, glycine increased MV productivity approximately 16-fold. Furthermore, glycine-induced MVs promoted interleukin-6 production by macrophage-like J774.1 cells, and the immunostimulatory activity was comparable to that of spontaneously produced MVs. Our results indicate that glycine is an effective agent for improving the production of MVs with immunostimulatory activity in gram-positive lactic acid bacteria, which can be applied as mucosal adjuvants and functional foods.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135496985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering microbial community dynamics along the fermentation course of soy sauce under different temperatures using metagenomic analysis. 利用宏基因组分析分析不同温度下酱油发酵过程中微生物群落动态。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2022-012

Nguyen Thanh Hai Nguyen, Ming Ban Huang, Fa Yong Liu, Wei-Ling Huang, Huyen-Trang Tran, Tsai-Wen Hsu, Chao-Li Huang, Tzen-Yuh Chiang

{"title":"Deciphering microbial community dynamics along the fermentation course of soy sauce under different temperatures using metagenomic analysis.","authors":"Nguyen Thanh Hai Nguyen, Ming Ban Huang, Fa Yong Liu, Wei-Ling Huang, Huyen-Trang Tran, Tsai-Wen Hsu, Chao-Li Huang, Tzen-Yuh Chiang","doi":"10.12938/bmfh.2022-012","DOIUrl":"https://doi.org/10.12938/bmfh.2022-012","url":null,"abstract":"Fermented soy sauce consists of microorganisms that exert beneficial effects. However, the microbial community dynamics during the fermentation course is poorly characterized. Soy sauce production is classified into the stages of mash fermentation with koji (S0), brine addition (S1), microbial transformation (S2), flavor creation (S3), and fermentation completion (S4). In this study, microbial succession was investigated across stages at different temperatures using metagenomics analyses. During mash fermentation, Aspergillus dominated the fungal microbiota in all stages, while the bacterial composition was dominated by Bacillus at room temperature and by a diverse composition of enriched lactic acid bacteria (LAB) at a controlled temperature. Compared with a stable fungal composition, bacterial dynamics were mostly attributable to fluctuations of LAB, which break down carbohydrates into lactic acid. After adding brine, increased levels of Enterococcus and decreased levels of Lactococcus from S1 to S4 may reflect differences in salinity tolerance. Staphylococcus, as a fermentation starter at S0, stayed predominant throughout fermentation and hydrolyzed soybean proteins. Meanwhile, Rhizopus and Penicillium may improve the flavor. The acidification of soy sauce was likely attributable to production of organic acids by Bacillus and LAB under room temperature and controlled temperature conditions, respectively. Metagenomic analysis revealed that microbial succession was associated with the fermentation efficiency and flavor enhancement. Controlled temperature nurture more LAB than uncontrolled temperatures and may ensure the production of lactic acid for the development of soy sauce flavor.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/83/bmfh-42-104.PMC10067331.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9258288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Bifidobacterium longum CLA8013 on bowel movement improvement: a placebo-controlled, randomized, double-blind study. 长双歧杆菌CLA8013对肠道运动改善的影响:一项安慰剂对照、随机、双盲研究。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2022-066

Keisuke Okada, Daisuke Takami, Yutaka Makizaki, Yoshiki Tanaka, Shunji Nakajima, Hiroshi Ohno, Toru Sagami

{"title":"Effects of Bifidobacterium longum CLA8013 on bowel movement improvement: a placebo-controlled, randomized, double-blind study.","authors":"Keisuke Okada, Daisuke Takami, Yutaka Makizaki, Yoshiki Tanaka, Shunji Nakajima, Hiroshi Ohno, Toru Sagami","doi":"10.12938/bmfh.2022-066","DOIUrl":"https://doi.org/10.12938/bmfh.2022-066","url":null,"abstract":"A placebo-controlled, randomized, double-blind study was conducted to evaluate the effect of taking 25 billion colony-forming units of heat-killed Bifidobacterium longum CLA8013 over 2 weeks on bowel movements in constipation-prone healthy individuals. The primary endpoint was the change in defecation frequency between the baseline and 2 weeks after the intake of B. longum CLA8013. The secondary endpoints were the number of days of defecation, stool volume, stool consistency, straining during defecation, pain during defecation, feeling of incomplete evacuation after defecation, abdominal bloating, fecal water content, and the Japanese version of the Patient Assessment of Constipation Quality of Life. A total of 120 individuals were assigned to two groups, 104 (control group, n=51; treatment group, n=53) of whom were included in the analysis. After 2 weeks of consuming the heat-killed B. longum CLA8013, defecation frequency increased significantly in the treatment group compared with that in the control group. Furthermore, compared with the control group, the treatment group showed a significant increase in stool volume and significant improvement in stool consistency, straining during defecation, and pain during defecation. No adverse events attributable to the heat-killed B. longum CLA8013 were observed during the study period. This study revealed that heat-killed B. longum CLA8013 improved the bowel movements of constipation-prone healthy individuals and confirmed that there were no relevant safety issues.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/06/bmfh-42-213.PMC10315193.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9801498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice. 含有牛蒡素的牛蒡芽提取物通过调节小鼠肠道微生物群来预防肥胖。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2021-070

Shimpei Watanabe, Akiko Ohno, Satoshi Yomoda, Satoshi Inamasu

{"title":"Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice.","authors":"Shimpei Watanabe, Akiko Ohno, Satoshi Yomoda, Satoshi Inamasu","doi":"10.12938/bmfh.2021-070","DOIUrl":"https://doi.org/10.12938/bmfh.2021-070","url":null,"abstract":"Several studies have suggested that the gut microbiota affect the health of the host. For example, the Firmicutes/Bacteroidetes (F/B) ratio and the proportion of Akkermansia muciniphila in the microbiota have been closely linked to obesity. In this study, we evaluated the effects of an anti-obesity lignan compound, arctigenin (AG), and burdock sprout extract (GSE), which contains AG, on the gut microbiota of an obese mouse model. C57BL/6J mice were fed high-fat, high-sucrose (HFHS) diets containing AG, GSE, or metformin (MF) for 8 weeks. The composition of the gut microbiota and the cecal content of short-chain fatty acids (SCFAs) were determined using 16S rRNA gene sequencing and high-performance liquid chromatography, respectively. Body weight gain was significantly suppressed in mice treated with AG, GSE, and MF. Analysis of the gut microbiota revealed that the F/B ratio was significantly reduced in the AG- and GSE-treated groups. Furthermore, the copy number of A. muciniphila in the feces was significantly increased in obese mice treated with AG and GSE. In addition, the amount of SCFAs (acetic, propionic, and butyric acids) in the cecal content and their fecal excretions were also significantly increased following AG and GSE treatment. Taken together, these results suggest that AG and GSE prevent obesity by improving the composition of the gut microbiota. Moreover, AG promoted the growth of A. muciniphila in vitro. Thus, AG and GSE may function as novel prebiotic supplements to ameliorate obesity, constipation, and intestinal disorders.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/d5/bmfh-42-049.PMC9816047.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9117425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Camellia oil (Camellia oleifera Abel.) treatment improves high-fat diet-induced atherosclerosis in apolipoprotein E (ApoE)^-/- mice. 山茶油(Camellia oleifera Abel.)治疗可改善载脂蛋白E (ApoE)-/-小鼠高脂肪饮食诱导的动脉粥样硬化。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2022-005

Tianyang Huang, Jianhui Jiang, YongJun Cao, Junze Huang, Fuan Zhang, Guozhen Cui

{"title":"Camellia oil (Camellia oleifera Abel.) treatment improves high-fat diet-induced atherosclerosis in apolipoprotein E (ApoE)-/- mice.","authors":"Tianyang Huang, Jianhui Jiang, YongJun Cao, Junze Huang, Fuan Zhang, Guozhen Cui","doi":"10.12938/bmfh.2022-005","DOIUrl":"https://doi.org/10.12938/bmfh.2022-005","url":null,"abstract":"Atherosclerosis is the main cause of cardiovascular diseases, and healthy dietary habits are a feasible strategy to prevent atherosclerosis development. Camellia oil, an edible plant oil, exhibits multiple beneficial cardiovascular effects. Our previous study showed that oral administration of camellia oil attenuated hyperglycemia, fat deposits in the liver, and the atherosclerosis index in high-fat diet (HFD)-induced obese mice. Here, an atherosclerosis model of apolipoprotein E (ApoE)-/- mice induced by HFD was used to study the effect of camellia oil on atherosclerosis, and 16S rRNA gene sequencing was used to analyze the changes in gut microbiota composition. The results showed that camellia oil significantly inhibited the formation of atherosclerotic plaques in ApoE-/- mice, which were characterized by significantly reduced levels of serum total cholesterol and enhanced levels of serum high-density lipoprotein cholesterol. The aortic levels of interleukin-6 and tumor necrosis factor were decreased. The results of the 16S rRNA analysis showed that after camellia oil interventions, the intestinal flora of ApoE-/- mice changed significantly, with the diversity of intestinal flora especially increasing, the relative abundances of Bacteroides, Faecalibaculum, Bilophila, and Leuconostoc increasing, and the Firmicutes/Bacteroidetes ratio and Firmicutes abundance decreasing. Collectively, our findings confirmed the promising value of camellia oil in preventing the development of atherosclerosis in ApoE-/- mice. Mechanistically, this preventive effect of camellia oil was probably due to its lipid-lowering activity, anti-inflammatory effects, and alteration of the gut microbiota composition in the mice.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/68/db/bmfh-42-056.PMC9816045.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10607726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Heterogeneity of gut microbiome compositions in the third decade of life in Japanese women: insights from a comparative analysis 日本女性30岁时肠道微生物组组成的异质性:来自比较分析的见解

4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2023-043

Tan Minh LE, Hong Duc Thi NGUYEN, Olive EM LEE, Donghyeon LEE, Yeseul CHOI, Gun Oh CHONG, Junghwan CHO, Nora Jee-Young PARK, Hyung Soo HAN, Incheol SEO

{"title":"Heterogeneity of gut microbiome compositions in the third decade of life in Japanese women: insights from a comparative analysis","authors":"Tan Minh LE, Hong Duc Thi NGUYEN, Olive EM LEE, Donghyeon LEE, Yeseul CHOI, Gun Oh CHONG, Junghwan CHO, Nora Jee-Young PARK, Hyung Soo HAN, Incheol SEO","doi":"10.12938/bmfh.2023-043","DOIUrl":"https://doi.org/10.12938/bmfh.2023-043","url":null,"abstract":"The reasons for sex-associated gut microbiota differences have not been determined, and although sex hormones, diet, and other factors are considered to contribute to them, many of these factors are age related. To shed light on this complex interplay, our study aimed to investigate and compare the gut microbial compositions of males and females across a broad range of ages, aiming to identify sex-associated disparities and potential causal factors. Our study encompassed a comprehensive analysis of gut microbiota data obtained from 444 Japanese individuals, ranging from newborns to centenarians, sourced from the DNA Data Bank of Japan. We categorized the subjects into 13 distinct age groups and examined their relative microbial abundances, as well as alpha and beta diversities, in relation to sex and age. No difference was observed between gut microbiota relative abundances or alpha diversities between men and women at any age. However, the study showed that the heterogeneity of gut microbiota among women in their 20s was greater than in men. To confirm the general occurrence of this difference, we conducted additional analyses using seven datasets: three from Japan and four from other countries. Interestingly, this variance was particularly noticeable within Japanese women. We also showed a potential link between the observed heterogeneity and dietary fiber intake. It is hoped this study will provide clues that aid in the identification of factors responsible for sex-associated differences in gut microbiota compositions.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135262048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of food-grade iron(Ⅲ) oxide nanoparticles on cecal digesta- and mucosa-associated microbiota and short-chain fatty acids in rats 食品级氧化铁(Ⅲ)纳米颗粒对大鼠盲肠食糜和粘膜相关微生物群和短链脂肪酸的影响

4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2023-012

Jiangchun SHI, Yumeng XIE, Yulin LI, Dongxia REN, Yiqi ZHANG, Huangfang SHAO, Yang LIU, Xue WANG, Yun LI

{"title":"Effects of food-grade iron(Ⅲ) oxide nanoparticles on cecal digesta- and mucosa-associated microbiota and short-chain fatty acids in rats","authors":"Jiangchun SHI, Yumeng XIE, Yulin LI, Dongxia REN, Yiqi ZHANG, Huangfang SHAO, Yang LIU, Xue WANG, Yun LI","doi":"10.12938/bmfh.2023-012","DOIUrl":"https://doi.org/10.12938/bmfh.2023-012","url":null,"abstract":"Although iron(Ⅲ) oxide nanoparticles (IONPs) are widely used in diverse applications ranging from food to biomedicine, the effects of IONPs on different locations of gut microbiota and short-chain fatty acids (SCFAs) are unclear. So, a subacute repeated oral toxicity study on Sprague Dawley (SD) rats was performed, administering low (50 mg/kg·bw), medium (100 mg/kg·bw), and high (200 mg/kg·bw) doses of IONPs. In this study, we found that a high dose of IONPs increased animal weight, and 16S rRNA sequencing revealed that IONPs caused intestinal flora disorders in both the cecal digesta- and mucosa-associated microbiota. However, only high-dose IONP exposure changed the abundance and composition of the mucosa-associated microbiota. IONPs increased the relative abundances of Firmicutes, Ruminococcaceae_UCG-014, Ruminiclostridium_9, Romboutsia, and Bilophila and decreased the relative abundance of Bifidobacterium, and many of these microorganisms are associated with weight gain, obesity, inflammation, diabetes, and mucosal damage. Functional analysis showed that changes in the gut microbiota induced by a high dose of IONPs were mainly related to metabolism, infection, immune, and endocrine disease functions. IONPs significantly elevated the levels of valeric, isobutyric, and isovaleric acid, promoting the absorption of iron. This is the first description of intestinal microbiota dysbiosis in SD rats caused by IONPs, and the effects and mechanisms of action of IONPs on intestinal and host health need to be further studied and confirmed.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135358925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin up-regulates the expression of tumor-suppressive microRNAs in human cervical cancer. 槲皮素上调人宫颈癌肿瘤抑制microrna的表达。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI: 10.12938/bmfh.2022-056

Motoki Murata, Satomi Komatsu, Emi Miyamoto, Chihiro Oka, Ichian Lin, Motofumi Kumazoe, Shuya Yamashita, Yoshinori Fujimura, Hirofumi Tachibana

{"title":"Quercetin up-regulates the expression of tumor-suppressive microRNAs in human cervical cancer.","authors":"Motoki Murata, Satomi Komatsu, Emi Miyamoto, Chihiro Oka, Ichian Lin, Motofumi Kumazoe, Shuya Yamashita, Yoshinori Fujimura, Hirofumi Tachibana","doi":"10.12938/bmfh.2022-056","DOIUrl":"https://doi.org/10.12938/bmfh.2022-056","url":null,"abstract":"Quercetin, a flavonol present in many vegetables and fruits, has been identified as a chemoprevention agent in several cancer models. However, the molecular mechanism of quercetin's anticancer activity is not entirely understood. MicroRNAs (miRNAs), small noncoding RNAs, have been reported to play key roles in various biological processes by regulating their target genes. We hypothesized that quercetin can exert an anticancer effect through the regulation of miRNAs. To test this hypothesis, we investigated the effects of quercetin on the expression of tumor-suppressive miRNAs in cervical cancer. Quercetin up-regulated the in vivo and in vitro expression of tumor-suppressive miRNAs miR-26b, miR-126, and miR-320a. Quercetin suppressed the level of β-catenin, encoded by catenin beta 1 (CTNNB1), by up-regulating miR-320a in HeLa cells. Moreover, quercetin increased the expression of mir-26b, mir-126, and mir-320a precursors in HeLa cells. The results from this study show that quercetin has the potential to prevent cervical cancer by regulating the expression of tumor-suppressive miRNAs.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bc/0f/bmfh-42-087.PMC9816044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10607725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue 局部游离脂肪酸触发小鼠白色脂肪组织中脂多糖结合蛋白的表达

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2022-01-28 DOI: 10.12938/bmfh.2021-061

Manami Seki, Akiho Miwa, Fumina Ohsaka, Yugo Karatsu, Takeshi Tsuruta, S. Hino, T. Morita, K. Sonoyama

{"title":"Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue","authors":"Manami Seki, Akiho Miwa, Fumina Ohsaka, Yugo Karatsu, Takeshi Tsuruta, S. Hino, T. Morita, K. Sonoyama","doi":"10.12938/bmfh.2021-061","DOIUrl":"https://doi.org/10.12938/bmfh.2021-061","url":null,"abstract":"Although lipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase protein mainly produced by hepatocytes, it has also been proposed to be a pro-inflammatory adipokine. Obesity and the consumption of a high-fat diet (HFD) are reportedly associated with elevated levels of LPS in plasma and free fatty acids (FFAs) in white adipose tissue (WAT). We examined whether circulating LPS or local FFAs are responsible for the HFD-induced increase of LBP in WAT. Male C57BL/6J mice were fed either a normal-fat diet (NFD) or an HFD. The mRNA levels in the liver and mesenteric WAT (mWAT), total FFA content in mWAT, and LBP and LPS concentrations in plasma were determined. The Lbp mRNA level in mWAT was higher in mice fed the HFD than in those fed the NFD for 3, 7, or 28 days or 14 weeks, whereas the hepatic Lbp mRNA level did not differ between the groups. The Lbp mRNA level in mWAT was also increased by the HFD in germ-free mice, which do not have gut microbiota, the source of LPS. The plasma LPS level did not show a significant correlation with the mWAT Lbp mRNA level. The total FFA content in mWAT was higher in mice fed the HFD than in those fed the NFD and positively correlated with the Lbp mRNA level. Supplementation with palmitic acid increased the Lbp mRNA level in 3T3-L1 adipocytes. We propose that local FFAs, but not circulating LPS, are the trigger for increased Lbp expression in mWAT of mice fed the HFD.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89873434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2