Effects of food-grade iron(Ⅲ) oxide nanoparticles on cecal digesta- and mucosa-associated microbiota and short-chain fatty acids in rats

IF 3.1 4区医学 Q2 Agricultural and Biological Sciences

Bioscience of Microbiota, Food and Health Pub Date : 2023-01-01 DOI:10.12938/bmfh.2023-012

Jiangchun SHI, Yumeng XIE, Yulin LI, Dongxia REN, Yiqi ZHANG, Huangfang SHAO, Yang LIU, Xue WANG, Yun LI

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引用次数: 0

Abstract

Although iron(Ⅲ) oxide nanoparticles (IONPs) are widely used in diverse applications ranging from food to biomedicine, the effects of IONPs on different locations of gut microbiota and short-chain fatty acids (SCFAs) are unclear. So, a subacute repeated oral toxicity study on Sprague Dawley (SD) rats was performed, administering low (50 mg/kg·bw), medium (100 mg/kg·bw), and high (200 mg/kg·bw) doses of IONPs. In this study, we found that a high dose of IONPs increased animal weight, and 16S rRNA sequencing revealed that IONPs caused intestinal flora disorders in both the cecal digesta- and mucosa-associated microbiota. However, only high-dose IONP exposure changed the abundance and composition of the mucosa-associated microbiota. IONPs increased the relative abundances of Firmicutes, Ruminococcaceae_UCG-014, Ruminiclostridium_9, Romboutsia, and Bilophila and decreased the relative abundance of Bifidobacterium, and many of these microorganisms are associated with weight gain, obesity, inflammation, diabetes, and mucosal damage. Functional analysis showed that changes in the gut microbiota induced by a high dose of IONPs were mainly related to metabolism, infection, immune, and endocrine disease functions. IONPs significantly elevated the levels of valeric, isobutyric, and isovaleric acid, promoting the absorption of iron. This is the first description of intestinal microbiota dysbiosis in SD rats caused by IONPs, and the effects and mechanisms of action of IONPs on intestinal and host health need to be further studied and confirmed.

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食品级氧化铁(Ⅲ)纳米颗粒对大鼠盲肠食糜和粘膜相关微生物群和短链脂肪酸的影响

虽然氧化铁纳米颗粒(Ⅲ)广泛应用于从食品到生物医学的各种应用中，但其对肠道微生物群和短链脂肪酸(SCFAs)不同部位的影响尚不清楚。为此，我们对SD大鼠进行了亚急性反复口服IONPs的毒性研究，分别给予低剂量(50 mg/kg·bw)、中剂量(100 mg/kg·bw)和高剂量(200 mg/kg·bw) IONPs。在这项研究中，我们发现高剂量的IONPs会增加动物体重，16S rRNA测序显示IONPs会导致盲肠消化和粘膜相关微生物群的肠道菌群紊乱。然而，只有高剂量的IONP暴露改变了粘膜相关微生物群的丰度和组成。IONPs增加了厚壁菌门(Firmicutes)、Ruminococcaceae_UCG-014、Ruminiclostridium_9、Romboutsia和Bilophila的相对丰度，降低了双歧杆菌(Bifidobacterium)的相对丰度，其中许多微生物与体重增加、肥胖、炎症、糖尿病和粘膜损伤有关。功能分析显示，高剂量IONPs诱导的肠道菌群变化主要与代谢、感染、免疫和内分泌疾病功能有关。IONPs显著提高了戊酸、异丁酸和异戊酸的水平，促进了铁的吸收。这是首次对离子离子ps引起SD大鼠肠道菌群失调的描述，离子离子ps对肠道和宿主健康的影响和作用机制有待进一步研究和证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

5.50

自引率

3.20%

发文量

期刊介绍： Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.